                                SEQUENCE LISTING

<110> Ho, Mitchell
      Pastan, Ira
      Kaneko, Osamu
      Lee, Byungkook
      Gong, Lucy
      The Government of the United States of America
         as represented by The Secretary of the
         Department of Health and Human Services

<120> Blocking Mesothelin Peptide Fragments
  

<130> 015280-563000PC

<140> WO PCT/US08/000000  
<141> 2008-12-04  

<160> 42

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 630
<212> PRT
<213> Homo sapiens

<220> 
<223> human mesothelin (MSLN, hnMSLN)

<400> 1
Met Ala Leu Pro Thr Ala Arg Pro Leu Leu Gly Ser Cys Gly Thr Pro
 1               5                  10                  15      
Ala Leu Gly Ser Leu Leu Phe Leu Leu Phe Ser Leu Gly Trp Val Gln
            20                  25                  30          
Pro Ser Arg Thr Leu Ala Gly Glu Thr Gly Gln Glu Ala Ala Pro Leu
        35                  40                  45              
Asp Gly Val Leu Ala Asn Pro Pro Asn Ile Ser Ser Leu Ser Pro Arg
    50                  55                  60                  
Gln Leu Leu Gly Phe Pro Cys Ala Glu Val Ser Gly Leu Ser Thr Glu
65                  70                  75                  80  
Arg Val Arg Glu Leu Ala Val Ala Leu Ala Gln Lys Asn Val Lys Leu
                85                  90                  95      
Ser Thr Glu Gln Leu Arg Cys Leu Ala His Arg Leu Ser Glu Pro Pro
            100                 105                 110         
Glu Asp Leu Asp Ala Leu Pro Leu Asp Leu Leu Leu Phe Leu Asn Pro
        115                 120                 125             
Asp Ala Phe Ser Gly Pro Gln Ala Cys Thr Arg Phe Phe Ser Arg Ile
    130                 135                 140                 
Thr Lys Ala Asn Val Asp Leu Leu Pro Arg Gly Ala Pro Glu Arg Gln
145                 150                 155                 160 
Arg Leu Leu Pro Ala Ala Leu Ala Cys Trp Gly Val Arg Gly Ser Leu
                165                 170                 175     
Leu Ser Glu Ala Asp Val Arg Ala Leu Gly Gly Leu Ala Cys Asp Leu
            180                 185                 190         
Pro Gly Arg Phe Val Ala Glu Ser Ala Glu Val Leu Leu Pro Arg Leu
        195                 200                 205             
Val Ser Cys Pro Gly Pro Leu Asp Gln Asp Gln Gln Glu Ala Ala Arg
    210                 215                 220                 
Ala Ala Leu Gln Gly Gly Gly Pro Pro Tyr Gly Pro Pro Ser Thr Trp
225                 230                 235                 240 
Ser Val Ser Thr Met Asp Ala Leu Arg Gly Leu Leu Pro Val Leu Gly
                245                 250                 255     
Gln Pro Ile Ile Arg Ser Ile Pro Gln Gly Ile Val Ala Ala Trp Arg
            260                 265                 270         
Gln Arg Ser Ser Arg Asp Pro Ser Trp Arg Gln Pro Glu Arg Thr Ile
        275                 280                 285             
Leu Arg Pro Arg Phe Arg Arg Glu Val Glu Lys Thr Ala Cys Pro Ser
    290                 295                 300                 
Gly Lys Lys Ala Arg Glu Ile Asp Glu Ser Leu Ile Phe Tyr Lys Lys
305                 310                 315                 320 
Trp Glu Leu Glu Ala Cys Val Asp Ala Ala Leu Leu Ala Thr Gln Met
                325                 330                 335     
Asp Arg Val Asn Ala Ile Pro Phe Thr Tyr Glu Gln Leu Asp Val Leu
            340                 345                 350         
Lys His Lys Leu Asp Glu Leu Tyr Pro Gln Gly Tyr Pro Glu Ser Val
        355                 360                 365             
Ile Gln His Leu Gly Tyr Leu Phe Leu Lys Met Ser Pro Glu Asp Ile
    370                 375                 380                 
Arg Lys Trp Asn Val Thr Ser Leu Glu Thr Leu Lys Ala Leu Leu Glu
385                 390                 395                 400 
Val Asn Lys Gly His Glu Met Ser Pro Gln Ala Pro Arg Arg Pro Leu
                405                 410                 415     
Pro Gln Val Ala Thr Leu Ile Asp Arg Phe Val Lys Gly Arg Gly Gln
            420                 425                 430         
Leu Asp Lys Asp Thr Leu Asp Thr Leu Thr Ala Phe Tyr Pro Gly Tyr
        435                 440                 445             
Leu Cys Ser Leu Ser Pro Glu Glu Leu Ser Ser Val Pro Pro Ser Ser
    450                 455                 460                 
Ile Trp Ala Val Arg Pro Gln Asp Leu Asp Thr Cys Asp Pro Arg Gln
465                 470                 475                 480 
Leu Asp Val Leu Tyr Pro Lys Ala Arg Leu Ala Phe Gln Asn Met Asn
                485                 490                 495     
Gly Ser Glu Tyr Phe Val Lys Ile Gln Ser Phe Leu Gly Gly Ala Pro
            500                 505                 510         
Thr Glu Asp Leu Lys Ala Leu Ser Gln Gln Asn Val Ser Met Asp Leu
        515                 520                 525             
Ala Thr Phe Met Lys Leu Arg Thr Asp Ala Val Leu Pro Leu Thr Val
    530                 535                 540                 
Ala Glu Val Gln Lys Leu Leu Gly Pro His Val Glu Gly Leu Lys Ala
545                 550                 555                 560 
Glu Glu Arg His Arg Pro Val Arg Asp Trp Ile Leu Arg Gln Arg Gln
                565                 570                 575     
Asp Asp Leu Asp Thr Leu Gly Leu Gly Leu Gln Gly Gly Ile Pro Asn
            580                 585                 590         
Gly Tyr Leu Val Leu Asp Leu Ser Met Gln Glu Ala Leu Ser Gly Thr
        595                 600                 605             
Pro Cys Leu Leu Gly Pro Gly Pro Val Leu Thr Val Leu Ala Leu Leu
    610                 615                 620                 
Leu Ala Ser Thr Leu Ala
625                 630 


<210> 2
<211> 64
<212> PRT
<213> Artificial Sequence

<220> 
<223> human mesothelin fragment residues 296-359, Region
      IAB

<220> 
<221> VARIANT        
<222> (26)...(59)
<223> Xaa = any amino acid

<400> 2
Glu Val Glu Lys Thr Ala Cys Pro Ser Gly Lys Lys Ala Arg Glu Ile
 1               5                  10                  15      
Asp Glu Ser Leu Ile Phe Tyr Lys Lys Xaa Glu Leu Xaa Ala Cys Val
            20                  25                  30          
Asp Ala Ala Leu Leu Ala Thr Gln Met Asp Arg Val Asn Ala Ile Pro
        35                  40                  45              
Phe Thr Tyr Glu Gln Leu Asp Val Leu Lys Xaa Lys Leu Asp Glu Leu
    50                  55                  60                  


<210> 3
<211> 64
<212> PRT
<213> Artificial Sequence

<220> 
<223> human mesothelin fragment residues 296-359, Region IAB

<400> 3
Glu Val Glu Lys Thr Ala Cys Pro Ser Gly Lys Lys Ala Arg Glu Ile
 1               5                  10                  15      
Asp Glu Ser Leu Ile Phe Tyr Lys Lys Trp Glu Leu Glu Ala Cys Val
            20                  25                  30          
Asp Ala Ala Leu Leu Ala Thr Gln Met Asp Arg Val Asn Ala Ile Pro
        35                  40                  45              
Phe Thr Tyr Glu Gln Leu Asp Val Leu Lys His Lys Leu Asp Glu Leu
    50                  55                  60                  


<210> 4
<211> 95
<212> PRT
<213> Artificial Sequence

<220> 
<223> human mesothelin fragment residues 296-390, Region I

<220> 
<221> VARIANT        
<222> (26)...(59)
<223> Xaa = any amino acid

<400> 4
Glu Val Glu Lys Thr Ala Cys Pro Ser Gly Lys Lys Ala Arg Glu Ile
 1               5                  10                  15      
Asp Glu Ser Leu Ile Phe Tyr Lys Lys Xaa Glu Leu Xaa Ala Cys Val
            20                  25                  30          
Asp Ala Ala Leu Leu Ala Thr Gln Met Asp Arg Val Asn Ala Ile Pro
        35                  40                  45              
Phe Thr Tyr Glu Gln Leu Asp Val Leu Lys Xaa Lys Leu Asp Glu Leu
    50                  55                  60                  
Tyr Pro Gln Gly Tyr Pro Glu Ser Val Ile Gln His Leu Gly Tyr Leu
65                  70                  75                  80  
Phe Leu Lys Met Ser Pro Glu Asp Ile Arg Lys Trp Asn Val Thr
                85                  90                  95  


<210> 5
<211> 95
<212> PRT
<213> Artificial Sequence

<220> 
<223> human mesothelin fragment residues 296-390, Region I

<400> 5
Glu Val Glu Lys Thr Ala Cys Pro Ser Gly Lys Lys Ala Arg Glu Ile
 1               5                  10                  15      
Asp Glu Ser Leu Ile Phe Tyr Lys Lys Trp Glu Leu Glu Ala Cys Val
            20                  25                  30          
Asp Ala Ala Leu Leu Ala Thr Gln Met Asp Arg Val Asn Ala Ile Pro
        35                  40                  45              
Phe Thr Tyr Glu Gln Leu Asp Val Leu Lys His Lys Leu Asp Glu Leu
    50                  55                  60                  
Tyr Pro Gln Gly Tyr Pro Glu Ser Val Ile Gln His Leu Gly Tyr Leu
65                  70                  75                  80  
Phe Leu Lys Met Ser Pro Glu Asp Ile Arg Lys Trp Asn Val Thr
                85                  90                  95  


<210> 6
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> 6xHis tag, His tag

<400> 6
His His His His His His
 1               5      


<210> 7
<211> 327
<212> PRT
<213> Artificial Sequence

<220> 
<223> human mesothelin fragment starting at Region I

<400> 7
Glu Val Glu Lys Thr Ala Cys Pro Ser Gly Lys Lys Ala Arg Glu Ile
 1               5                  10                  15      
Asp Glu Ser Leu Ile Phe Tyr Lys Lys Trp Glu Leu Glu Ala Cys Val
            20                  25                  30          
Asp Ala Ala Leu Leu Ala Thr Gln Met Asp Arg Val Asn Ala Ile Pro
        35                  40                  45              
Phe Thr Tyr Glu Gln Leu Asp Val Leu Lys His Lys Leu Asp Glu Leu
    50                  55                  60                  
Tyr Pro Gln Gly Tyr Pro Glu Ser Val Ile Gln His Leu Gly Tyr Leu
65                  70                  75                  80  
Phe Leu Lys Met Ser Pro Glu Asp Ile Arg Lys Trp Asn Val Thr Ser
                85                  90                  95      
Leu Glu Thr Leu Lys Ala Leu Leu Glu Val Asn Lys Gly His Glu Met
            100                 105                 110         
Ser Pro Gln Val Ala Thr Leu Ile Asp Arg Phe Val Lys Gly Arg Gly
        115                 120                 125             
Gln Leu Asp Lys Asp Thr Leu Asp Thr Leu Thr Ala Phe Tyr Pro Gly
    130                 135                 140                 
Tyr Leu Cys Ser Leu Ser Pro Glu Glu Leu Ser Ser Val Pro Pro Ser
145                 150                 155                 160 
Ser Ile Trp Ala Val Arg Pro Gln Asp Leu Asp Thr Cys Asp Pro Arg
                165                 170                 175     
Gln Leu Asp Val Leu Tyr Pro Lys Ala Arg Leu Ala Phe Gln Asn Met
            180                 185                 190         
Asn Gly Ser Glu Tyr Phe Val Lys Ile Gln Ser Phe Leu Gly Gly Ala
        195                 200                 205             
Pro Thr Glu Asp Leu Lys Ala Leu Ser Gln Gln Asn Val Ser Met Asp
    210                 215                 220                 
Leu Ala Thr Phe Met Lys Leu Arg Thr Asp Ala Val Leu Pro Leu Thr
225                 230                 235                 240 
Val Ala Glu Val Gln Lys Leu Leu Gly Pro His Val Glu Gly Leu Lys
                245                 250                 255     
Ala Glu Glu Arg His Arg Pro Val Arg Asp Trp Ile Leu Arg Gln Arg
            260                 265                 270         
Gln Asp Asp Leu Asp Thr Leu Gly Leu Gly Leu Gln Gly Gly Ile Pro
        275                 280                 285             
Asn Gly Tyr Leu Val Leu Asp Leu Ser Val Gln Glu Ala Leu Ser Gly
    290                 295                 300                 
Thr Pro Cys Leu Leu Gly Pro Gly Pro Val Leu Thr Val Leu Ala Leu
305                 310                 315                 320 
Leu Leu Ala Ser Thr Leu Ala
                325         


<210> 8
<211> 328
<212> PRT
<213> Artificial Sequence

<220> 
<223> mouse mesothelin fragment starting at Region I

<400> 8
Asp Ala Glu Gln Lys Ala Cys Pro Pro Gly Lys Glu Pro Tyr Lys Val
 1               5                  10                  15      
Asp Glu Asp Leu Ile Phe Tyr Gln Asn Trp Glu Leu Glu Ala Cys Val
            20                  25                  30          
Asp Gly Thr Met Leu Ala Arg Gln Met Asp Leu Val Asn Glu Ile Pro
        35                  40                  45              
Phe Thr Tyr Glu Gln Leu Ser Ile Phe Lys His Lys Leu Asp Lys Thr
    50                  55                  60                  
Tyr Pro Gln Gly Tyr Pro Glu Ser Leu Ile Gln Gln Leu Gly His Phe
65                  70                  75                  80  
Phe Arg Tyr Val Ser Pro Glu Asp Ile His Gln Trp Asn Val Thr Ser
                85                  90                  95      
Pro Asp Thr Val Lys Thr Leu Leu Lys Val Ser Lys Gly Gln Lys Met
            100                 105                 110         
Asn Ala Gln Ala Ile Ala Leu Val Ala Cys Tyr Leu Arg Gly Gly Gly
        115                 120                 125             
Gln Leu Asp Glu Asp Met Val Lys Ala Leu Gly Asp Ile Pro Leu Ser
    130                 135                 140                 
Tyr Leu Cys Asp Phe Ser Pro Gln Asp Leu His Ser Val Pro Ser Ser
145                 150                 155                 160 
Val Met Trp Leu Val Gly Pro Gln Asp Leu Asp Lys Cys Ser Gln Arg
                165                 170                 175     
His Leu Gly Leu Leu Tyr Gln Lys Ala Cys Ser Ala Phe Gln Asn Val
            180                 185                 190         
Ser Gly Leu Glu Tyr Phe Glu Lys Ile Lys Thr Phe Leu Gly Gly Ala
        195                 200                 205             
Ser Val Lys Asp Leu Arg Ala Leu Ser Gln His Asn Val Ser Met Asp
    210                 215                 220                 
Ile Ala Thr Phe Lys Arg Leu Gln Val Asp Ser Leu Val Gly Leu Ser
225                 230                 235                 240 
Val Ala Glu Val Gln Lys Leu Leu Gly Pro Asn Ile Val Asp Leu Lys
                245                 250                 255     
Thr Glu Glu Asp Lys Ser Pro Val Arg Asp Trp Leu Phe Arg Gln His
            260                 265                 270         
Gln Lys Asp Leu Asp Arg Leu Gly Leu Gly Leu Gln Gly Gly Ile Pro
        275                 280                 285             
Asn Gly Tyr Leu Val Leu Asp Phe Asn Val Arg Glu Ala Phe Ser Ser
    290                 295                 300                 
Arg Ala Ser Leu Leu Gly Pro Gly Phe Val Leu Ile Trp Ile Pro Ala
305                 310                 315                 320 
Leu Leu Pro Ala Leu Arg Leu Ser
                325             


<210> 9
<211> 328
<212> PRT
<213> Artificial Sequence

<220> 
<223> consensus sequence of human and mouse mesothelin
      fragment starting at Region I

<220> 
<221> VARIANT        
<222> (2)...(2)
<223> Xaa = Val or Ala

<220> 
<221> VARIANT        
<222> (4)...(4)
<223> Xaa = Lys or Gln

<220> 
<221> VARIANT        
<222> (5)...(5)
<223> Xaa = Thr or Lys

<220> 
<221> VARIANT        
<222> (9)...(9)
<223> Xaa = Ser or Pro

<220> 
<221> VARIANT        
<222> (12)...(12)
<223> Xaa = Lys or Gln

<220> 
<221> VARIANT        
<222> (13)...(13)
<223> Xaa = Ala or Pro

<220> 
<221> VARIANT        
<222> (14)...(14)
<223> Xaa = Arg or Tyr

<220> 
<221> VARIANT        
<222> (15)...(15)
<223> Xaa = Glu or Lys

<220> 
<221> VARIANT        
<222> (19)...(19)
<223> Xaa = Ser or Asp

<220> 
<221> VARIANT        
<222> (24)...(24)
<223> Xaa = Lys or Gln

<220> 
<221> VARIANT        
<222> (25)...(25)
<223> Xaa = Lys or Asn

<220> 
<221> VARIANT        
<222> (35)...(35)
<223> Xaa = Ala or Thr

<220> 
<221> VARIANT        
<222> (39)...(39)
<223> Xaa = Thr or Arg

<220> 
<221> VARIANT        
<222> (43)...(43)
<223> Xaa = Arg or Leu

<220> 
<221> VARIANT        
<222> (46)...(46)
<223> Xaa = Ala or Glu

<220> 
<221> VARIANT        
<222> (55)...(55)
<223> Xaa = Asp or Ser

<220> 
<221> VARIANT        
<222> (57)...(57)
<223> Xaa = Leu or Phe

<220> 
<221> VARIANT        
<222> (57)...(57)
<223> Xaa = Leu or Phe

<220> 
<221> VARIANT        
<222> (63)...(63)
<223> Xaa = Glu or Lys

<220> 
<221> VARIANT        
<222> (64)...(64)
<223> Xaa = Leu or Thr

<220> 
<221> VARIANT        
<222> (76)...(76)
<223> Xaa = His or Gln

<220> 
<221> VARIANT        
<222> (79)...(79)
<223> Xaa = Tyr or His

<220> 
<221> VARIANT        
<222> (80)...(80)
<223> Xaa = Leu or Phe

<220> 
<221> VARIANT        
<222> (82)...(82)
<223> Xaa = Leu or Arg

<220> 
<221> VARIANT        
<222> (83)...(83)
<223> Xaa = Lys or Tyr

<220> 
<221> VARIANT        
<222> (90)...(90)
<223> Xaa = Arg or His

<220> 
<221> VARIANT        
<222> (91)...(91)
<223> Xaa = Lys or Gln

<220> 
<221> VARIANT        
<222> (97)...(97)
<223> Xaa = Leu or Pro

<220> 
<221> VARIANT        
<222> (102)...(102)
<223> Xaa = Ala or Thr

<220> 
<221> VARIANT        
<222> (105)...(105)
<223> Xaa = Glu or Lys

<220> 
<221> VARIANT        
<222> (107)...(107)
<223> Xaa = Asn or Ser

<220> 
<221> VARIANT        
<222> (110)...(110)
<223> Xaa = His or Gln

<220> 
<221> VARIANT        
<222> (111)...(111)
<223> Xaa = Glu or Lys

<220> 
<221> VARIANT        
<222> (113)...(113)
<223> Xaa = Ser or Asn

<220> 
<221> VARIANT        
<222> (114)...(114)
<223> Xaa = Pro or Ala

<220> 
<221> VARIANT        
<222> (116)...(116)
<223> Xaa = Val or Ala

<220> 
<221> VARIANT        
<222> (117)...(117)
<223> Xaa = Ala or Ile

<220> 
<221> VARIANT        
<222> (118)...(118)
<223> Xaa = Thr or Ala

<220> 
<221> VARIANT        
<222> (121)...(121)
<223> Xaa = Asp or Ala

<220> 
<221> VARIANT        
<222> (122)...(122)
<223> Xaa = Arg or Cys

<220> 
<221> VARIANT        
<222> (127)...(127)
<223> Xaa = Arg or Gly

<220> 
<221> VARIANT        
<222> (132)...(132)
<223> Xaa = Lys or Glu

<220> 
<221> VARIANT        
<222> (134)...(134)
<223> Xaa = Thr or Met

<220> 
<221> VARIANT        
<222> (136)...(136)
<223> Xaa = Asp or Lys

<220> 
<221> VARIANT        
<222> (137)...(137)
<223> Xaa = Thr or Ala

<220> 
<221> VARIANT        
<222> (139)...(139)
<223> Xaa = Thr or Gly

<220> 
<221> VARIANT        
<222> (140)...(140)
<223> Xaa = Ala or Asp

<220> 
<221> VARIANT        
<222> (140)...(140)
<223> Xaa = Ala or Asp

<220> 
<221> VARIANT        
<222> (141)...(141)
<223> Xaa = Phe or Ile

<220> 
<221> VARIANT        
<222> (142)...(142)
<223> Xaa = Tyr or Pro

<220> 
<221> VARIANT        
<222> (143)...(143)
<223> Xaa = Pro or Leu

<220> 
<221> VARIANT        
<222> (144)...(144)
<223> Xaa = Gly or Ser

<220> 
<221> VARIANT        
<222> (148)...(148)
<223> Xaa = Ser or Asp

<220> 
<221> VARIANT        
<222> (149)...(149)
<223> Xaa = Leu or Phe

<220> 
<221> VARIANT        
<222> (155)...(155)
<223> Xaa = Ser or His

<220> 
<221> VARIANT        
<222> (159)...(159)
<223> Xaa = Pro or Ser

<220> 
<221> VARIANT        
<222> (161)...(161)
<223> Xaa = Ser or Val

<220> 
<221> VARIANT        
<222> (164)...(164)
<223> Xaa = Ala or Leu

<220> 
<221> VARIANT        
<222> (166)...(166)
<223> Xaa = Arg or Gly

<220> 
<221> VARIANT        
<222> (172)...(172)
<223> Xaa = Thr or Lys

<220> 
<221> VARIANT        
<222> (174)...(174)
<223> Xaa = Asp or Ser

<220> 
<221> VARIANT        
<222> (175)...(175)
<223> Xaa = Pro or Gln

<220> 
<221> VARIANT        
<222> (177)...(177)
<223> Xaa = Gln or His

<220> 
<221> VARIANT        
<222> (179)...(179)
<223> Xaa = Asp or Gly

<220> 
<221> VARIANT        
<222> (183)...(183)
<223> Xaa = Pro or Gln

<220> 
<221> VARIANT        
<222> (186)...(186)
<223> Xaa = Arg or Cys

<220> 
<221> VARIANT        
<222> (187)...(187)
<223> Xaa = Leu or Ser

<220> 
<221> VARIANT        
<222> (193)...(193)
<223> Xaa = Asn or Ser

<220> 
<221> VARIANT        
<222> (195)...(195)
<223> Xaa = Ser or Leu

<220> 
<221> VARIANT        
<222> (199)...(199)
<223> Xaa = Val or Glu

<220> 
<221> VARIANT        
<222> (202)...(202)
<223> Xaa = Gln or Lys

<220> 
<221> VARIANT        
<222> (209)...(209)
<223> Xaa = Pro or Ser

<220> 
<221> VARIANT        
<222> (210)...(210)
<223> Xaa = Thr or Val

<220> 
<221> VARIANT        
<222> (211)...(211)
<223> Xaa = Glu or Lys

<220> 
<221> VARIANT        
<222> (219)...(219)
<223> Xaa = Gln or His

<220>
<221> VARIANT        
<222> (229)...(229)
<223> Xaa = Met or Lys

<220> 
<221> VARIANT        
<222> (232)...(232)
<223> Xaa = Arg or Gln

<220> 
<221> VARIANT        
<222> (233)...(233)
<223> Xaa = Thr or Val

<220> 
<221> VARIANT        
<222> (235)...(235)
<223> Xaa = Ala or Ser

<220> 
<221> VARIANT        
<222> (238)...(238)
<223> Xaa = Pro or Gly

<220> 
<221> VARIANT        
<222> (251)...(251)
<223> Xaa = His or Asn

<220> 
<221> VARIANT        
<222> (253)...(253)
<223> Xaa = Gln or Val

<220> 
<221> VARIANT        
<222> (254)...(254)
<223> Xaa = Gly or Asp

<220> 
<221> VARIANT        
<222> (257)...(257)
<223> Xaa = Ala or Thr

<220> 
<221> VARIANT        
<222> (260)...(260)
<223> Xaa = Arg or Asp

<220> 
<221> VARIANT        
<222> (261)...(261)
<223> Xaa = His or Lys

<220> 
<221> VARIANT        
<222> (262)...(262)
<223> Xaa = Arg or Ser

<220> 
<221> VARIANT        
<222> (269)...(269)
<223> Xaa = Leu or Phe

<220> 
<221> VARIANT        
<222> (272)...(272)
<223> Xaa = Arg or His

<220> 
<221> VARIANT        
<222> (274)...(274)
<223> Xaa = Asp or Lys

<220> 
<221> VARIANT        
<222> (278)...(278)
<223> Xaa = Thr or Arg

<220> 
<221> VARIANT        
<222> (296)...(296)
<223> Xaa = Leu or Phe

<220> 
<221> VARIANT        
<222> (297)...(297)
<223> Xaa = Ser or Asn

<220> 
<221> VARIANT        
<222> (299)...(299)
<223> Xaa = Gln or Arg

<220> 
<221> VARIANT        
<222> (302)...(302)
<223> Xaa = Leu or Phe

<220> 
<221> VARIANT        
<222> (304)...(304)
<223> Xaa = Gly or Ser

<220> 
<221> VARIANT        
<222> (305)...(305)
<223> Xaa = Thr or Arg

<220> 
<221> VARIANT        
<222> (306)...(306)
<223> Xaa = Pro or Ala

<220> 
<221> VARIANT        
<222> (307)...(307)
<223> Xaa = Cys or Ser

<220> 
<221> VARIANT        
<222> (313)...(313)
<223> Xaa = Pro or Phe

<220> 
<221> VARIANT        
<222> (316)...(316)
<223> Xaa = Thr or Ile

<220> 
<221> VARIANT        
<222> (319)...(319)
<223> Xaa = Leu or Pro

<220> 
<221> VARIANT        
<222> (323)...(323)
<223> Xaa = Leu or Pro

<220> 
<221> VARIANT        
<222> (325)...(325)
<223> Xaa = Ser or Leu

<220> 
<221> VARIANT        
<222> (326)...(326)
<223> Xaa = Thr or Arg

<220> 
<221> VARIANT        
<222> (328)...(328)
<223> Xaa = Ala or Ser

<400> 9
Glu Xaa Glu Xaa Xaa Ala Cys Pro Xaa Gly Lys Xaa Xaa Xaa Xaa Ile
 1               5                  10                  15      
Asp Glu Xaa Leu Ile Phe Tyr Xaa Xaa Trp Glu Leu Glu Ala Cys Val
            20                  25                  30          
Asp Ala Xaa Leu Leu Ala Xaa Gln Met Asp Xaa Val Asn Xaa Ile Pro
        35                  40                  45              
Phe Thr Tyr Glu Gln Leu Xaa Val Xaa Lys His Lys Leu Asp Xaa Xaa
    50                  55                  60                  
Tyr Pro Gln Gly Tyr Pro Glu Ser Val Ile Gln Xaa Leu Gly Xaa Xaa
65                  70                  75                  80  
Phe Xaa Xaa Met Ser Pro Glu Asp Ile Xaa Xaa Trp Asn Val Thr Ser
                85                  90                  95      
Xaa Glu Thr Leu Lys Xaa Leu Leu Xaa Val Xaa Lys Gly Xaa Xaa Met
            100                 105                 110         
Xaa Xaa Gln Xaa Xaa Xaa Leu Ile Xaa Xaa Phe Val Lys Gly Xaa Gly
        115                 120                 125             
Gln Leu Asp Xaa Asp Xaa Leu Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa
    130                 135                 140                 
Tyr Leu Cys Xaa Xaa Ser Pro Glx Glu Leu Xaa Ser Val Pro Xaa Ser
145                 150                 155                 160 
Xaa Ile Trp Xaa Val Xaa Pro Gln Asp Leu Asp Xaa Cys Xaa Xaa Arg
                165                 170                 175     
Xaa Leu Xaa Val Leu Tyr Xaa Lys Ala Xaa Xaa Ala Phe Gln Asn Met
            180                 185                 190         
Xaa Gly Xaa Glu Tyr Phe Xaa Lys Ile Xaa Ser Phe Leu Gly Gly Ala
        195                 200                 205             
Xaa Xaa Xaa Asp Leu Lys Ala Leu Ser Gln Xaa Asn Val Ser Met Asp
    210                 215                 220                 
Leu Ala Thr Phe Xaa Lys Leu Xaa Xaa Asp Xaa Val Leu Xaa Leu Thr
225                 230                 235                 240 
Val Ala Glu Val Gln Lys Leu Leu Gly Pro Xaa Val Xaa Xaa Leu Lys
                245                 250                 255     
Xaa Glu Glu Xaa Xaa Xaa Pro Val Arg Asp Trp Ile Xaa Arg Gln Xaa
            260                 265                 270         
Gln Xaa Asp Leu Asp Xaa Leu Gly Leu Gly Leu Gln Gly Gly Ile Pro
        275                 280                 285             
Asn Gly Tyr Leu Val Leu Asp Xaa Xaa Val Xaa Glu Ala Xaa Ser Xaa
    290                 295                 300                 
Xaa Xaa Xaa Leu Leu Gly Pro Gly Xaa Val Leu Xaa Trp Val Xaa Ala
305                 310                 315                 320 
Leu Leu Xaa Ala Xaa Xaa Leu Xaa
                325             


<210> 10
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> artificial T-cell epitope, MHC Class II pan DR
      epitope, PADRE peptide

<220> 
<221> VARIANT        
<222> (3)...(3)
<223> Xaa = cyclohexylalanine, Tyr or Phe

<400> 10
Ala Lys Xaa Val Ala Ala Trp Thr Leu Lys Ala Ala Ala
 1               5                  10              


<210> 11
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region I (296-390) fragment forward primer used to
      construct truncated mesothelin mutant

<400> 11
agaagaagag aattcgaagt ggagaagaca gcctgt                           36

<210> 12
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region I (296-390) fragment reverse primer used to
      construct truncated mesothelin mutant

<400> 12
ctcttcttct gcggccgccg tcacattcca cttgcgaat                        39

<210> 13
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region II (391-486) fragment forward primer used
      to construct truncated mesothelin mutant

<400> 13
agaagaagag aattctccct ggagaccctg aaggct                           36

<210> 14
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region II (391-486) fragment reverse primer used
      to construct truncated mesothelin mutant

<400> 14
ctcttcttct gcggccgcct ggaaagcaag gcgggcctt                        39

<210> 15
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region III (487-581) fragment forward primer used
      to construct truncated mesothelin mutant

<400> 15
agaagaagag aattcaacat gaacgggtcc gaatac                           36

<210> 16
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region III (487-581) fragment reverse primer used
      to construct truncated mesothelin mutant

<400> 16
ctcttcttct gcggccgcgc cctgtagccc cagccccag                        39

<210> 17
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region IAB (296-359) fragment forward primer used
      to construct truncated mesothelin mutant

<400> 17
agaagaagag aattcgaagt ggagaagaca gcctgt                           36

<210> 18
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region IAB (296-359) fragment reverse primer used
      to construct truncated mesothelin mutant

<400> 18
ctcttcttct gcggccgcga gctcatccag tttatgctt                        39

<210> 19
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region IBC (328-405) fragment forward primer used
      to construct truncated mesothelin mutant

<400> 19
agaagaagag aattcgatgc ggccctgctg gccacc                           36

<210> 20
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region IBC (328-405) fragment reverse primer used
      to construct truncated mesothelin mutant

<400> 20
ctcttcttct gcggccgcgt gccctttgtt gacttcaag                        39

<210> 21
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region IA (296-337) fragment forward primer used
      to construct truncated mesothelin mutant

<400> 21
agaagaagaa agcttgaagt ggagaagaca gcctgt                           36

<210> 22
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region IA (296-337) fragment reverse primer used
      to construct truncated mesothelin mutant

<400> 22
tcttcttctg gatccgtcca tctgggtggc cagcag                           36

<210> 23
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region IB (328-369) fragment forward primer used
      to construct truncated mesothelin mutant

<400> 23
agaagaagag aattcgatgc ggccctgctg gccacc                           36

<210> 24
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region IB (328-369) fragment reverse primer used
      to construct truncated mesothelin mutant

<400> 24
ctcttcttct gcggccgcga tcacagactc ggggtaacc                        39

<210> 25
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region IC (360-405) fragment forward primer used
      to construct truncated mesothelin mutant

<400> 25
agaagaagag aattctaccc acaaggttac cccgag                           36

<210> 26
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Region IC (360-405) fragment reverse primer used
      to construct truncated mesothelin mutant

<400> 26
ctcttcttct gcggccgcgt gccctttgtt gacttcaag                        39

<210> 27
<211> 93
<212> DNA
<213> Artificial Sequence

<220> 
<223> Y318A forward PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 27
agaagagaat tcgaagtgga gaagacagcc tgtccttcag gcaagaaggc ccgcgagata 60
gacgagagcc tcatcttcgc caagaagtgg gag                              93

<210> 28
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Y318A reverse PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 28
ctcttcttct gcggccgcga gctcatccag tttatgctt                        39

<210> 29
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> W321A forward PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 29
tcatcttcta caagaaggcg gagctggaag cctgcgtgg                        39

<210> 30
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> W321A reverse PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 30
ccacgcaggc ttccagctcc gccttcttgt agaagatga                        39

<210> 31
<211> 108
<212> DNA
<213> Artificial Sequence

<220> 
<223> E324A forward PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 31
agaagagaat tcgaagtgga gaagacagcc tgtccttcag gcaagaaggc ccgcgagata 60
gacgagagcc tcatcttcta caagaagtgg gagctggcag cctgcgtg              108

<210> 32
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> E324A reverse PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 32
ctcttcttct gcggccgcga gctcatccag tttatgctt                        39

<210> 33
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> F344A forward PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 33
agaagaagag aattcgaagt ggagaagaca gcctgt                           36

<210> 34
<211> 75
<212> DNA
<213> Artificial Sequence

<220> 
<223> F344A reverse PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 34
ttcttctgcg gccgcgagct catccagttt atgctttagg acgtccagct gctcgtaggt 60
ggcggggatg gcgtt                                                  75

<210> 35
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> E347A forward PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 35
agaagaagag aattcgaagt ggagaagaca gcctgt                           36

<210> 36
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> E347A reverse PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 36
ttcttctgcg gccgcgagct catccagttt atgctttagg acgtccagct gcgcgtaggt 60
gaaggg                                                            66

<210> 37
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> K353A forward PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 37
agaagaagag aattcgaagt ggagaagaca gcctgt                           36

<210> 38
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> K353A reverse PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 38
ttcttctgcg gccgcgagct catccagttt atgcgctagg acgtccag              48

<210> 39
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> H354A forward PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 39
agaagaagag aattcgaagt ggagaagaca gcctgt                           36

<210> 40
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> H354A reverse PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 40
ctcttcttct gcggccgcga gctcatccag tttagccttt aggacgtc              48

<210> 41
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> K355A forward PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 41
agaagaagag aattcgaagt ggagaagaca gcctgt                           36

<210> 42
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> K355A reverse PCR mutagenesis primer used to
      generate alanine replacement mesothelin mutant

<400> 42
ctcttcttct gcggccgcga gctcatccag tgcatgcttt aggacgtc              48


