                         SEQUENCE LISTING

<110>  Henkel AG & Co. KGaA
 
<120>  Wasch- und Reinigungsmittel enthaltend Proteasen aus Bacillus 
       pumilus

<130>  H 08234 PCT

<150>  DE 102008059447.4
<151>  2008-11-27

<160>  4     

<170>  PatentIn version 3.3

<210>  1
<211>  1152
<212>  DNA
<213>  Bacillus pumilus


<220>
<221>  CDS
<222>  (1)..(1149)

<220>
<221>  mat_peptide
<222>  (325)..()

<400>  1
atg tgc gtg aaa  aag aaa aat gtg atg  aca agt gtt tta ttg gct gtc     48
Met Cys Val Lys  Lys Lys Asn Val Met  Thr Ser Val Leu Leu Ala Val       
            -105                 -100                 -95               

cct ctt ctg ttt tca gca ggg ttt gga gga tcc atg gca aat gcc gaa       96
Pro Leu Leu Phe Ser Ala Gly Phe Gly Gly Ser Met Ala Asn Ala Glu         
        -90                 -85                 -80                     

acg gtc tcc aaa aca gat agt gaa aaa agc tat att gtt ggt ttt aaa      144
Thr Val Ser Lys Thr Asp Ser Glu Lys Ser Tyr Ile Val Gly Phe Lys         
    -75                 -70                 -65                         

gcc tct gcc acc aca aac agc tct aag aaa caa gct gtc att caa aat      192
Ala Ser Ala Thr Thr Asn Ser Ser Lys Lys Gln Ala Val Ile Gln Asn         
-60                 -55                 -50                 -45         

ggt gga aaa cta gaa aaa caa tac cgc ctc att aat gct gca caa gtg      240
Gly Gly Lys Leu Glu Lys Gln Tyr Arg Leu Ile Asn Ala Ala Gln Val         
                -40                 -35                 -30             

aaa atg tcc gaa caa gcc gcc aag aaa ctt gaa cat gac cct agc att      288
Lys Met Ser Glu Gln Ala Ala Lys Lys Leu Glu His Asp Pro Ser Ile         
            -25                 -20                 -15                 

gct tac gta gaa gaa gac cat aaa gca gaa gca tat gca caa acc gtc      336
Ala Tyr Val Glu Glu Asp His Lys Ala Glu Ala Tyr Ala Gln Thr Val         
        -10                 -5              -1  1                       

cct tat gga atc cct caa atc aaa gct cca gct gta cac gct caa ggt      384
Pro Tyr Gly Ile Pro Gln Ile Lys Ala Pro Ala Val His Ala Gln Gly         
5                   10                  15                  20          

tat aaa ggt gct aat gtc aaa gta gct gtc ctt gat act gga atc cac      432
Tyr Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp Thr Gly Ile His         
                25                  30                  35              

gct gca cac cct gac tta aat gtt gca ggc ggt gcg agc ttc gtc cct      480
Ala Ala His Pro Asp Leu Asn Val Ala Gly Gly Ala Ser Phe Val Pro         
            40                  45                  50                  

tca gag cca aat gcc acc caa gac ttt caa tca cat gga act cac gta      528
Ser Glu Pro Asn Ala Thr Gln Asp Phe Gln Ser His Gly Thr His Val         
        55                  60                  65                      

gca gga acc att gct gcc ctt gat aac aca att ggt gtt ctt ggg gtc      576
Ala Gly Thr Ile Ala Ala Leu Asp Asn Thr Ile Gly Val Leu Gly Val         
    70                  75                  80                          

gct cca agc gct tcc cta tat gct gtg aaa gta tta gac cgt aat ggc      624
Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu Asp Arg Asn Gly         
85                  90                  95                  100         

gac gga caa tac agc tgg att att agc ggt att gaa tgg gct gta gcg      672
Asp Gly Gln Tyr Ser Trp Ile Ile Ser Gly Ile Glu Trp Ala Val Ala         
                105                 110                 115             

aat aac atg gat gtc atc aat atg agc tta ggc gga cca aac ggc tcc      720
Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro Asn Gly Ser         
            120                 125                 130                 

aca gcg ctt aaa aat gct gtt gac aca gcg aat aac cgc gga gta gtt      768
Thr Ala Leu Lys Asn Ala Val Asp Thr Ala Asn Asn Arg Gly Val Val         
        135                 140                 145                     

gtc gtt gcc gca gca gga aat tca ggt tca ttt ggc tct act agc acc      816
Val Val Ala Ala Ala Gly Asn Ser Gly Ser Phe Gly Ser Thr Ser Thr         
    150                 155                 160                         

gtt ggc tat cca gca aaa tac gat tct aca att gct gtt gcc aat gta      864
Val Gly Tyr Pro Ala Lys Tyr Asp Ser Thr Ile Ala Val Ala Asn Val         
165                 170                 175                 180         

aac agt aac aat gtc aga aac tca tct tct agc gca ggt cct gaa tta      912
Asn Ser Asn Asn Val Arg Asn Ser Ser Ser Ser Ala Gly Pro Glu Leu         
                185                 190                 195             

gat gtt tct gca cct ggt act tct att tta agt acg gtg cca agc agt      960
Asp Val Ser Ala Pro Gly Thr Ser Ile Leu Ser Thr Val Pro Ser Ser         
            200                 205                 210                 

gga tac act tct tat act gga aca tct atg gcg tct cct cat gta gca     1008
Gly Tyr Thr Ser Tyr Thr Gly Thr Ser Met Ala Ser Pro His Val Ala         
        215                 220                 225                     

gga gca gca gcg ctt atc ctt tct aag tat ccg aat cta tca act tct     1056
Gly Ala Ala Ala Leu Ile Leu Ser Lys Tyr Pro Asn Leu Ser Thr Ser         
    230                 235                 240                         

cag gtt cgc cag cgc tta gaa aac acg gca aca ccg ctt ggt gac tca     1104
Gln Val Arg Gln Arg Leu Glu Asn Thr Ala Thr Pro Leu Gly Asp Ser         
245                 250                 255                 260         

ttc tat tac gga aaa ggg tta atc aac gtt caa gcg gct tct aac taa     1152
Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala Ala Ser Asn             
                265                 270                 275             


<210>  2
<211>  383
<212>  PRT
<213>  Bacillus pumilus

<400>  2

Met Cys Val Lys  Lys Lys Asn Val Met  Thr Ser Val Leu Leu Ala Val 
            -105                 -100                 -95         


Pro Leu Leu Phe Ser Ala Gly Phe Gly Gly Ser Met Ala Asn Ala Glu 
        -90                 -85                 -80             


Thr Val Ser Lys Thr Asp Ser Glu Lys Ser Tyr Ile Val Gly Phe Lys 
    -75                 -70                 -65                 


Ala Ser Ala Thr Thr Asn Ser Ser Lys Lys Gln Ala Val Ile Gln Asn 
-60                 -55                 -50                 -45 


Gly Gly Lys Leu Glu Lys Gln Tyr Arg Leu Ile Asn Ala Ala Gln Val 
                -40                 -35                 -30     


Lys Met Ser Glu Gln Ala Ala Lys Lys Leu Glu His Asp Pro Ser Ile 
            -25                 -20                 -15         


Ala Tyr Val Glu Glu Asp His Lys Ala Glu Ala Tyr Ala Gln Thr Val 
        -10                 -5              -1  1               


Pro Tyr Gly Ile Pro Gln Ile Lys Ala Pro Ala Val His Ala Gln Gly 
5                   10                  15                  20  


Tyr Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp Thr Gly Ile His 
                25                  30                  35      


Ala Ala His Pro Asp Leu Asn Val Ala Gly Gly Ala Ser Phe Val Pro 
            40                  45                  50          


Ser Glu Pro Asn Ala Thr Gln Asp Phe Gln Ser His Gly Thr His Val 
        55                  60                  65              


Ala Gly Thr Ile Ala Ala Leu Asp Asn Thr Ile Gly Val Leu Gly Val 
    70                  75                  80                  


Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu Asp Arg Asn Gly 
85                  90                  95                  100 


Asp Gly Gln Tyr Ser Trp Ile Ile Ser Gly Ile Glu Trp Ala Val Ala 
                105                 110                 115     


Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro Asn Gly Ser 
            120                 125                 130         


Thr Ala Leu Lys Asn Ala Val Asp Thr Ala Asn Asn Arg Gly Val Val 
        135                 140                 145             


Val Val Ala Ala Ala Gly Asn Ser Gly Ser Phe Gly Ser Thr Ser Thr 
    150                 155                 160                 


Val Gly Tyr Pro Ala Lys Tyr Asp Ser Thr Ile Ala Val Ala Asn Val 
165                 170                 175                 180 


Asn Ser Asn Asn Val Arg Asn Ser Ser Ser Ser Ala Gly Pro Glu Leu 
                185                 190                 195     


Asp Val Ser Ala Pro Gly Thr Ser Ile Leu Ser Thr Val Pro Ser Ser 
            200                 205                 210         


Gly Tyr Thr Ser Tyr Thr Gly Thr Ser Met Ala Ser Pro His Val Ala 
        215                 220                 225             


Gly Ala Ala Ala Leu Ile Leu Ser Lys Tyr Pro Asn Leu Ser Thr Ser 
    230                 235                 240                 


Gln Val Arg Gln Arg Leu Glu Asn Thr Ala Thr Pro Leu Gly Asp Ser 
245                 250                 255                 260 


Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala Ala Ser Asn 
                265                 270                 275 


<210>  3
<211>  275
<212>  PRT
<213>  Bacillus pumilus

<400>  3

Ala Gln Thr Val Pro Tyr Gly Ile Pro Gln Ile Lys Ala Pro Ala Val 
1               5                   10                  15      


His Ala Gln Gly Tyr Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile His Ala Ala His Pro Asp Leu Asn Val Ala Gly Gly Ala 
        35                  40                  45              


Ser Phe Val Pro Ser Glu Pro Asn Ala Thr Gln Asp Phe Gln Ser His 
    50                  55                  60                  


Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asp Asn Thr Ile Gly 
65                  70                  75                  80  


Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      


Asp Arg Asn Gly Asp Gly Gln Tyr Ser Trp Ile Ile Ser Gly Ile Glu 
            100                 105                 110         


Trp Ala Val Ala Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             


Pro Asn Gly Ser Thr Ala Leu Lys Asn Ala Val Asp Thr Ala Asn Asn 
    130                 135                 140                 


Arg Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Phe Gly 
145                 150                 155                 160 


Ser Thr Ser Thr Val Gly Tyr Pro Ala Lys Tyr Asp Ser Thr Ile Ala 
                165                 170                 175     


Val Ala Asn Val Asn Ser Asn Asn Val Arg Asn Ser Ser Ser Ser Ala 
            180                 185                 190         


Gly Pro Glu Leu Asp Val Ser Ala Pro Gly Thr Ser Ile Leu Ser Thr 
        195                 200                 205             


Val Pro Ser Ser Gly Tyr Thr Ser Tyr Thr Gly Thr Ser Met Ala Ser 
    210                 215                 220                 


Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys Tyr Pro Asn 
225                 230                 235                 240 


Leu Ser Thr Ser Gln Val Arg Gln Arg Leu Glu Asn Thr Ala Thr Pro 
                245                 250                 255     


Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         


Ala Ser Asn 
        275 


<210>  4
<211>  269
<212>  PRT
<213>  Bacillus lentus

<400>  4

Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala 
1               5                   10                  15      


His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Asp Gly Arg Gly Ala Ile Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 
        115                 120                 125             


Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Ser Ser Ile Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 
        195                 200                 205             


Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 
                245                 250                 255     


Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


