                                SEQUENCE LISTING

<110> Liu, Ning
      Academia, Katrina
      Paulus, Aran
      Wehr, Tim
      Walker, John A., II
      Freeby, Steve
      Bio-Rad Laboratories, Inc.

<120> Phosphopeptide Enrichment of
  Compositions by Fractionation on Ceramic Hydroxyapatite
  

<130> 002558-084010PC

<140> WO PCT/US09/64116   
<141> 2009-11-12  

<150> US 61/114,801       
<151> 2008-11-14  

<160> 10

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> alpha-casein trypsin digest flow-through fraction
      peptide

<400> 1
Tyr Leu Gly Tyr Leu Glu Gln Leu Leu Arg
 1               5                  10  


<210> 2
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> alpha-casein trypsin digest flow-through fraction
      peptide

<400> 2
Phe Phe Val Ala Pro Phe Pro Glu Val Phe Gly Lys
 1               5                  10          


<210> 3
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> alpha-casein trypsin digest flow-through fraction
      peptide

<400> 3
His Gln Gly Leu Pro Gln Glu Val Leu Asn Glu Asn Leu Leu Arg
 1               5                  10                  15  


<210> 4
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> alpha-casein trypsin digest flow-through fraction
      peptide

<400> 4
Glu Pro Met Ile Glu Val Asn Gln Glu Leu Ala Tyr Phe Tyr Pro Glu
 1               5                  10                  15      
Leu Phe Arg
            


<210> 5
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> alpha-casein trypsin digest ceramic
      hydroxyapatitie (CHT) low-stringency eluate
      fraction singly phosphorylated peptide

<221> MOD_RES        
<222> (6)...(6)
<223> Ser = phosphoserine

<400> 5
Thr Val Asp Met Glu Ser Thr Glu Val Phe Thr Lys
 1               5                  10          


<210> 6
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> alpha-casein trypsin digest ceramic
      hydroxyapatitie (CHT) low-stringency eluate
      fraction singly phosphorylated peptide

<221> MOD_RES        
<222> (10)...(10)
<223> Ser = phosphoserine

<400> 6
Val Pro Gln Leu Glu Ile Val Pro Asn Ser Ala Glu Glu Arg
 1               5                  10                  


<210> 7
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> alpha-casein trypsin digest ceramic
      hydroxyapatitie (CHT) low-stringency eluate
      fraction singly phosphorylated peptide

<221> MOD_RES        
<222> (12)...(12)
<223> Ser = phosphoserine

<400> 7
Tyr Lys Val Pro Gln Leu Glu Ile Val Pro Asn Ser Ala Glu Glu Arg
 1               5                  10                  15      


<210> 8
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> alpha-casein trypsin digest ceramic
      hydroxyapatitie (CHT) high-stringency eluate
      fraction multiply phosphorylated peptide

<221> MOD_RES        
<222> (4)...(6)
<223> Ser = phosphoserine

<400> 8
Asp Ile Gly Ser Glu Ser Thr Glu Asp Gln Ala Met Glu Asp Ile Lys
 1               5                  10                  15      


<210> 9
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> alpha-casein trypsin digest ceramic
      hydroxyapatitie (CHT) high-stringency eluate
      fraction multiply phosphorylated peptide

<221> MOD_RES        
<222> (6)...(17)
<223> Ser = phosphoserine

<400> 9
Gln Met Glu Ala Glu Ser Ile Ser Ser Ser Glu Glu Ile Val Pro Asn
 1               5                  10                  15      
Ser Val Glu Gln Lys
            20      


<210> 10
<211> 25
<212> PRT
<213> Artificial Sequence

<220> 
<223> alpha-casein trypsin digest ceramic
      hydroxyapatitie (CHT) high-stringency eluate
      fraction multiply phosphorylated peptide

<221> MOD_RES        
<222> (11)...(16)
<223> Ser = phosphoserine

<400> 10
Asn Ala Asn Glu Glu Glu Tyr Ser Ile Gly Ser Ser Ser Glu Glu Ser
 1               5                  10                  15      
Ala Glu Val Ala Thr Glu Glu Val Lys
            20                  25  

