                         SEQUENCE LISTING

<110>  Abbott Laboratories
       Ali, Salman
       Bonn, Ryan M.
       Grenier, Frank C.
       Rae, Tracey D.
       Rupprecht, Kevin R.
       Syed, Hina N.
       Tu, Bailin
       Tyner, Joan D. 
       Workman, Ryan F.

 
<120>  NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN (NGAL) PROTEIN ISOFORMS ENRICHED FROM URINE AND RECOMBINANT CHINESE HAMSTER OVARY (CHO) CELLS AND RELATED COMPOSITIONS, ANTIBODIES, AND METHODS OF ENRICHMENT, ANALYSIS AND USE

<130>  9600WOO1

<150>  61/111,551
<151>  2008-11-05

<160>  6    

<170>  PatentIn version 3.4

<210>  1
<211>  198
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  The first 20 amino acids are the signal peptide which would be 
       labeled 1 to 20, with Gln (at position 21) being considered the 
       first amino acid of the mature NGAL peptide

<400>  1

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 
            20                  25                  30          


Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln 
        35                  40                  45              


Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu 
    50                  55                  60                  


Asp Lys Asp Pro Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu 
65                  70                  75                  80  


Asp Lys Ser Tyr Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys 
                85                  90                  95      


Asp Tyr Trp Ile Arg Thr Phe Val Pro Gly Cys Gln Pro Gly Glu Phe 
            100                 105                 110         


Thr Leu Gly Asn Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val 
        115                 120                 125             


Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 
    130                 135                 140                 


Lys Val Ser Gln Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg 
145                 150                 155                 160 


Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 
                165                 170                 175     


Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 
            180                 185                 190         


Asp Gln Cys Ile Asp Gly 
        195             



<210>  2
<211>  178
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Chemically synthesized


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Gln can be preceded by a Met residue (e.g., when synthetic and/or
       produced in prokaryotes.

<220>
<221>  MISC_FEATURE
<222>  (178)..(178)
<223>  Can, optionally, be succeeded at the C-terminus by one or more 
       His residues, and especially, 6 His residues (HHHHHH).

<400>  2

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 
65                  70                  75                  80  


Arg Thr Phe Val Pro Gly Cys Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      


Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Ser Gln 
        115                 120                 125             


Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  3
<211>  612
<212>  DNA
<213>  Homo sapiens

<400>  3
atgcccctag gtctcctgtg gctgggccta gccctgttgg gggctctgca tgcccaggcc     60

caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag    120

aacttccagg acaaccaatt ccaggggaag tggtatgtgg taggcctggc agggaatgca    180

attctcagag aagacaaaga cccgcaaaag atgtatgcca ccatctatga gctgaaagaa    240

gacaagagct acaatgtcac ctccgtcctg tttaggaaaa agaagtgtga ctactggatc    300

aggacttttg ttccaggttg ccagcccggc gagttcacgc tgggcaacat taagagttac    360

cctggattaa cgagttacct cgtccgagtg gtgagcacca actacaacca gcatgctatg    420

gtgttcttca agaaagtttc tcaaaacagg gagtacttca agatcaccct ctacgggaga    480

accaaggagc tgacttcgga actaaaggag aacttcatcc gcttctccaa atctctgggc    540

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggccatcat    600

caccatcacc at                                                        612

<210>  4
<211>  198
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Chemically synthesized


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  The first 20 amino acids are the signal peptide which would be 
       labeled -1 to -20, with Gln (at position 21) being considered 
       amino acid 1 of the NGAL peptide

<400>  4

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 
            20                  25                  30          


Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln 
        35                  40                  45              


Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu 
    50                  55                  60                  


Asp Lys Asp Pro Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu 
65                  70                  75                  80  


Asp Lys Ser Tyr Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys 
                85                  90                  95      


Asp Tyr Trp Ile Arg Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 
            100                 105                 110         


Thr Leu Gly Asn Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val 
        115                 120                 125             


Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 
    130                 135                 140                 


Lys Val Ser Gln Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg 
145                 150                 155                 160 


Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 
                165                 170                 175     


Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 
            180                 185                 190         


Asp Gln Cys Ile Asp Gly 
        195             





<210>  5
<211>  178
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Chemically synthesized


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Gln can be preceded by a Met residue (e.g., when synthetic and/or
       produced in prokaryotes)

<220>
<221>  MISC_FEATURE
<222>  (178)..(178)
<223>  Can, optionally, be succeeded at the C-terminus by one or more 
       His residues, and especially, 6 His residues (HHHHHH).

<400>  5

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      


Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              


Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  


Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 
65                  70                  75                  80  


Arg Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      


Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Ser Gln 
        115                 120                 125             


Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 


Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 


Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     


Asp Gly 
        


<210>  6
<211>  552
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Chemically synthesized


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Optionally, can be preceded at the N-terminus by an initiation 
       codon encoding Met, namely, an ATG.

<400>  6
caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag     60

aacttccagg acaaccaatt ccaggggaag tggtatgtgg taggcctggc agggaatgca    120

attctcagag aagacaaaga cccgcaaaag atgtatgcca ccatctatga gctgaaagaa    180

gacaagagct acaatgtcac ctccgtcctg tttaggaaaa agaagtgtga ctactggatc    240

aggacttttg ttccaggttc gcagcccggc gagttcacgc tgggcaacat taagagttac    300

cctggattaa cgagttacct cgtccgagtg gtgagcacca actacaacca gcatgctatg    360

gtgttcttca agaaagtttc tcaaaacagg gagtacttca agatcaccct ctacgggaga    420

accaaggagc tgacttcgga actaaaggag aacttcatcc gcttctccaa atctctgggc    480

ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggccatcat    540

caccatcacc at                                                        552






