                               SEQUENCE LISTING

<110> ALBITAR, MAHER
      MA, WANLONG
 
<120> COMPOSITIONS AND METHODS FOR DETECTING MUTATIONS IN JAK2 
      NUCLEIC ACID

<130> 034827-0651

<140> 12/503,318
<141> 2009-07-15

<150> 61/110,501
<151> 2008-10-31

<160> 11    

<170> PatentIn version 3.5

<210> 1
<211> 5097
<212> DNA
<213> Homo sapiens

<400> 1
ctgcaggaag gagagaggaa gaggagcaga agggggcagc agcggacgcc gctaacggcc       60

tccctcggcg ctgacaggct gggccggcgc ccggctcgct tgggtgttcg cgtcgccact      120

tcggcttctc ggccggtcgg gcccctcggc ccgggcttgc ggcgcgcgtc ggggctgagg      180

gctgctgcgg cgcagggaga ggcctggtcc tcgctgccga gggatgtgag tgggagctga      240

gcccacactg gagggccccc gagggcccag cctggaggtc gttcagagcc gtgcccgccc      300

cggggcttcg cagaccttga cccgccgggt aggagccgcc cctgcgggct cgagggcgcg      360

ctctggtcgc ccgatctgtg tagccggttt cagaagcagg caacaggaac aagatgtgaa      420

ctgtttctct tctgcagaaa aagaggctct tcctcctcct cccgcgacgg caaatgttct      480

gaaaaagact ctgcatggga atggcctgcc ttacgatgac agaaatggag ggaacatcca      540

cctcttctat atatcagaat ggtgatattt ctggaaatgc caattctatg aagcaaatag      600

atccagttct tcaggtgtat ctttaccatt cccttgggaa atctgaggca gattatctga      660

cctttccatc tggggagtat gttgcagaag aaatctgtat tgctgcttct aaagcttgtg      720

gtatcacacc tgtgtatcat aatatgtttg ctttaatgag tgaaacagaa aggatctggt      780

atccacccaa ccatgtcttc catatagatg agtcaaccag gcataatgta ctctacagaa      840

taagatttta ctttcctcgt tggtattgca gtggcagcaa cagagcctat cggcatggaa      900

tatctcgagg tgctgaagct cctcttcttg atgactttgt catgtcttac ctctttgctc      960

agtggcggca tgattttgtg cacggatgga taaaagtacc tgtgactcat gaaacacagg     1020

aagaatgtct tgggatggca gtgttagata tgatgagaat agccaaagaa aacgatcaaa     1080

ccccactggc catctataac tctatcagct acaagacatt cttaccaaaa tgtattcgag     1140

caaagatcca agactatcat attttgacaa ggaagcgaat aaggtacaga tttcgcagat     1200

ttattcagca attcagccaa tgcaaagcca ctgccagaaa cttgaaactt aagtatctta     1260

taaatctgga aactctgcag tctgccttct acacagagaa atttgaagta aaagaacctg     1320

gaagtggtcc ttcaggtgag gagatttttg caaccattat aataactgga aacggtggaa     1380

ttcagtggtc aagagggaaa cataaagaaa gtgagacact gacagaacag gatttacagt     1440

tatattgcga ttttcctaat attattgatg tcagtattaa gcaagcaaac caagagggtt     1500

caaatgaaag ccgagttgta actatccata agcaagatgg taaaaatctg gaaattgaac     1560

ttagctcatt aagggaagct ttgtctttcg tgtcattaat tgatggatat tatagattaa     1620

ctgcagatgc acatcattac ctctgtaaag aagtagcacc tccagccgtg cttgaaaata     1680

tacaaagcaa ctgtcatggc ccaatttcga tggattttgc cattagtaaa ctgaagaaag     1740

caggtaatca gactggactg tatgtacttc gatgcagtcc taaggacttt aataaatatt     1800

ttttgacttt tgctgtcgag cgagaaaatg tcattgaata taaacactgt ttgattacaa     1860

aaaatgagaa tgaagagtac aacctcagtg ggacaaagaa gaacttcagc agtcttaaag     1920

atcttttgaa ttgttaccag atggaaactg ttcgctcaga caatataatt ttccagttta     1980

ctaaatgctg tcccccaaag ccaaaagata aatcaaacct tctagtcttc agaacgaatg     2040

gtgtttctga tgtaccaacc tcaccaacat tacagaggcc tactcatatg aaccaaatgg     2100

tgtttcacaa aatcagaaat gaagatttga tatttaatga aagccttggc caaggcactt     2160

ttacaaagat ttttaaaggc gtacgaagag aagtaggaga ctacggtcaa ctgcatgaaa     2220

cagaagttct tttaaaagtt ctggataaag cacacagaaa ctattcagag tctttctttg     2280

aagcagcaag tatgatgagc aagctttctc acaagcattt ggttttaaat tatggagtat     2340

gtgtctgtgg agacgagaat attctggttc aggagtttgt aaaatttgga tcactagata     2400

catatctgaa aaagaataaa aattgtataa atatattatg gaaacttgaa gttgctaaac     2460

agttggcatg ggccatgcat tttctagaag aaaacaccct tattcatggg aatgtatgtg     2520

ccaaaaatat tctgcttatc agagaagaag acaggaagac aggaaatcct cctttcatca     2580

aacttagtga tcctggcatt agtattacag ttttgccaaa ggacattctt caggagagaa     2640

taccatgggt accacctgaa tgcattgaaa atcctaaaaa tttaaatttg gcaacagaca     2700

aatggagttt tggtaccact ttgtgggaaa tctgcagtgg aggagataaa cctctaagtg     2760

ctctggattc tcaaagaaag ctacaatttt atgaagatag gcatcagctt cctgcaccaa     2820

agtgggcaga attagcaaac cttataaata attgtatgga ttatgaacca gatttcaggc     2880

cttctttcag agccatcata cgagatctta acagtttgtt tactccagat tatgaactat     2940

taacagaaaa tgacatgtta ccaaatatga ggataggtgc cctagggttt tctggtgcct     3000

ttgaagaccg ggatcctaca cagtttgaag agagacattt gaaatttcta cagcaacttg     3060

gcaagggtaa ttttgggagt gtggagatgt gccggtatga ccctctacag gacaacactg     3120

gggaggtggt cgctgtaaaa aagcttcagc atagtactga agagcaccta agagactttg     3180

aaagggaaat tgaaatcctg aaatccctac agcatgacaa cattgtaaag tacaagggag     3240

tgtgctacag tgctggtcgg cgtaatctaa aattaattat ggaatattta ccatatggaa     3300

gtttacgaga ctatcttcaa aaacataaag aacggataga tcacataaaa cttctgcagt     3360

acacatctca gatatgcaag ggtatggagt atcttggtac aaaaaggtat atccacaggg     3420

atctggcaac gagaaatata ttggtggaga acgagaacag agttaaaatt ggagattttg     3480

ggttaaccaa agtcttgcca caagacaaag aatactataa agtaaaagaa cctggtgaaa     3540

gtcccatatt ctggtatgct ccagaatcac tgacagagag caagttttct gtggcctcag     3600

atgtttggag ctttggagtg gttctgtatg aacttttcac atacattgag aagagtaaaa     3660

gtccaccagc ggaatttatg cgtatgattg gcaatgacaa acaaggacag atgatcgtgt     3720

tccatttgat agaacttttg aagaataatg gaagattacc aagaccagat ggatgcccag     3780

atgagatcta tatgatcatg acagaatgct ggaacaataa tgtaaatcaa cgcccctcct     3840

ttagggatct agctcttcga gtggatcaaa taagggataa catggctgga tgaaagaaat     3900

gaccttcatt ctgagaccaa agtagattta cagaacaaag ttttatattt cacattgctg     3960

tggactatta ttacatatat cattattata taaatcatga tgctagccag caaagatgtg     4020

aaaatatctg ctcaaaactt tcaaagttta gtaagttttt cttcatgagg ccaccagtaa     4080

aagacattaa tgagaattcc ttagcaagga ttttgtaaga agtttcttaa acattgtctg     4140

ttaacatcac tcttgtctgg caaaagaaaa aaaatagact ttttcaactc agctttttga     4200

gacctgaaaa aattattatg taaattttgc aatgttaaag atgcacagaa tatgtatgta     4260

tagtttttac cacagtggat gtataatacc ttggcatctt gtgtgatgtt ttacacacat     4320

gagggctggt gttcattaat actgttttct aatttttcca tagttaatct ataattaatt     4380

acttcactat acaaacaaat taagatgttc agataattga ataagtacct ttgtgtcctt     4440

gttcatttat atcgctggcc agcattataa gcaggtgtat acttttagct tgtagttcca     4500

tgtactgtaa atatttttca cataaaggga acaaatgtct agttttattt gtataggaaa     4560

tttccctgac cctaaataat acattttgaa atgaaacaag cttacaaaga tataatctat     4620

tttattatgg tttcccttgt atctatttgt ggtgaatgtg ttttttaaat ggaactatct     4680

ccaaattttt ctaagactac tatgaacagt tttcttttaa aattttgaga ttaagaatgc     4740

caggaatatt gtcatccttt gagctgctga ctgccaataa cattcttcga tctctgggat     4800

ttatgctcat gaactaaatt taagcttaag ccataaaata gattagattg ttttttaaaa     4860

atggatagct cattaagaag tgcagcaggt taagaatttt ttcctaaaga ctgtatattt     4920

gaggggtttc agaattttgc attgcagtca tagaagagat ttatttcctt tttagagggg     4980

aaatgaggta aataagtaaa aaagtatgct tgttaatttt attcaagaat gccagtagaa     5040

aattcataac gtgtatcttt aagaaaaatg agcatacatc ttaaatcttt tcaatta        5097


<210> 2
<211> 1132
<212> PRT
<213> Homo sapiens

<400> 2
Met Gly Met Ala Cys Leu Thr Met Thr Glu Met Glu Gly Thr Ser Thr 
1               5                   10                  15      


Ser Ser Ile Tyr Gln Asn Gly Asp Ile Ser Gly Asn Ala Asn Ser Met 
            20                  25                  30          


Lys Gln Ile Asp Pro Val Leu Gln Val Tyr Leu Tyr His Ser Leu Gly 
        35                  40                  45              


Lys Ser Glu Ala Asp Tyr Leu Thr Phe Pro Ser Gly Glu Tyr Val Ala 
    50                  55                  60                  


Glu Glu Ile Cys Ile Ala Ala Ser Lys Ala Cys Gly Ile Thr Pro Val 
65                  70                  75                  80  


Tyr His Asn Met Phe Ala Leu Met Ser Glu Thr Glu Arg Ile Trp Tyr 
                85                  90                  95      


Pro Pro Asn His Val Phe His Ile Asp Glu Ser Thr Arg His Asn Val 
            100                 105                 110         


Leu Tyr Arg Ile Arg Phe Tyr Phe Pro Arg Trp Tyr Cys Ser Gly Ser 
        115                 120                 125             


Asn Arg Ala Tyr Arg His Gly Ile Ser Arg Gly Ala Glu Ala Pro Leu 
    130                 135                 140                 


Leu Asp Asp Phe Val Met Ser Tyr Leu Phe Ala Gln Trp Arg His Asp 
145                 150                 155                 160 


Phe Val His Gly Trp Ile Lys Val Pro Val Thr His Glu Thr Gln Glu 
                165                 170                 175     


Glu Cys Leu Gly Met Ala Val Leu Asp Met Met Arg Ile Ala Lys Glu 
            180                 185                 190         


Asn Asp Gln Thr Pro Leu Ala Ile Tyr Asn Ser Ile Ser Tyr Lys Thr 
        195                 200                 205             


Phe Leu Pro Lys Cys Ile Arg Ala Lys Ile Gln Asp Tyr His Ile Leu 
    210                 215                 220                 


Thr Arg Lys Arg Ile Arg Tyr Arg Phe Arg Arg Phe Ile Gln Gln Phe 
225                 230                 235                 240 


Ser Gln Cys Lys Ala Thr Ala Arg Asn Leu Lys Leu Lys Tyr Leu Ile 
                245                 250                 255     


Asn Leu Glu Thr Leu Gln Ser Ala Phe Tyr Thr Glu Lys Phe Glu Val 
            260                 265                 270         


Lys Glu Pro Gly Ser Gly Pro Ser Gly Glu Glu Ile Phe Ala Thr Ile 
        275                 280                 285             


Ile Ile Thr Gly Asn Gly Gly Ile Gln Trp Ser Arg Gly Lys His Lys 
    290                 295                 300                 


Glu Ser Glu Thr Leu Thr Glu Gln Asp Leu Gln Leu Tyr Cys Asp Phe 
305                 310                 315                 320 


Pro Asn Ile Ile Asp Val Ser Ile Lys Gln Ala Asn Gln Glu Gly Ser 
                325                 330                 335     


Asn Glu Ser Arg Val Val Thr Ile His Lys Gln Asp Gly Lys Asn Leu 
            340                 345                 350         


Glu Ile Glu Leu Ser Ser Leu Arg Glu Ala Leu Ser Phe Val Ser Leu 
        355                 360                 365             


Ile Asp Gly Tyr Tyr Arg Leu Thr Ala Asp Ala His His Tyr Leu Cys 
    370                 375                 380                 


Lys Glu Val Ala Pro Pro Ala Val Leu Glu Asn Ile Gln Ser Asn Cys 
385                 390                 395                 400 


His Gly Pro Ile Ser Met Asp Phe Ala Ile Ser Lys Leu Lys Lys Ala 
                405                 410                 415     


Gly Asn Gln Thr Gly Leu Tyr Val Leu Arg Cys Ser Pro Lys Asp Phe 
            420                 425                 430         


Asn Lys Tyr Phe Leu Thr Phe Ala Val Glu Arg Glu Asn Val Ile Glu 
        435                 440                 445             


Tyr Lys His Cys Leu Ile Thr Lys Asn Glu Asn Glu Glu Tyr Asn Leu 
    450                 455                 460                 


Ser Gly Thr Lys Lys Asn Phe Ser Ser Leu Lys Asp Leu Leu Asn Cys 
465                 470                 475                 480 


Tyr Gln Met Glu Thr Val Arg Ser Asp Asn Ile Ile Phe Gln Phe Thr 
                485                 490                 495     


Lys Cys Cys Pro Pro Lys Pro Lys Asp Lys Ser Asn Leu Leu Val Phe 
            500                 505                 510         


Arg Thr Asn Gly Val Ser Asp Val Pro Thr Ser Pro Thr Leu Gln Arg 
        515                 520                 525             


Pro Thr His Met Asn Gln Met Val Phe His Lys Ile Arg Asn Glu Asp 
    530                 535                 540                 


Leu Ile Phe Asn Glu Ser Leu Gly Gln Gly Thr Phe Thr Lys Ile Phe 
545                 550                 555                 560 


Lys Gly Val Arg Arg Glu Val Gly Asp Tyr Gly Gln Leu His Glu Thr 
                565                 570                 575     


Glu Val Leu Leu Lys Val Leu Asp Lys Ala His Arg Asn Tyr Ser Glu 
            580                 585                 590         


Ser Phe Phe Glu Ala Ala Ser Met Met Ser Lys Leu Ser His Lys His 
        595                 600                 605             


Leu Val Leu Asn Tyr Gly Val Cys Val Cys Gly Asp Glu Asn Ile Leu 
    610                 615                 620                 


Val Gln Glu Phe Val Lys Phe Gly Ser Leu Asp Thr Tyr Leu Lys Lys 
625                 630                 635                 640 


Asn Lys Asn Cys Ile Asn Ile Leu Trp Lys Leu Glu Val Ala Lys Gln 
                645                 650                 655     


Leu Ala Trp Ala Met His Phe Leu Glu Glu Asn Thr Leu Ile His Gly 
            660                 665                 670         


Asn Val Cys Ala Lys Asn Ile Leu Leu Ile Arg Glu Glu Asp Arg Lys 
        675                 680                 685             


Thr Gly Asn Pro Pro Phe Ile Lys Leu Ser Asp Pro Gly Ile Ser Ile 
    690                 695                 700                 


Thr Val Leu Pro Lys Asp Ile Leu Gln Glu Arg Ile Pro Trp Val Pro 
705                 710                 715                 720 


Pro Glu Cys Ile Glu Asn Pro Lys Asn Leu Asn Leu Ala Thr Asp Lys 
                725                 730                 735     


Trp Ser Phe Gly Thr Thr Leu Trp Glu Ile Cys Ser Gly Gly Asp Lys 
            740                 745                 750         


Pro Leu Ser Ala Leu Asp Ser Gln Arg Lys Leu Gln Phe Tyr Glu Asp 
        755                 760                 765             


Arg His Gln Leu Pro Ala Pro Lys Trp Ala Glu Leu Ala Asn Leu Ile 
    770                 775                 780                 


Asn Asn Cys Met Asp Tyr Glu Pro Asp Phe Arg Pro Ser Phe Arg Ala 
785                 790                 795                 800 


Ile Ile Arg Asp Leu Asn Ser Leu Phe Thr Pro Asp Tyr Glu Leu Leu 
                805                 810                 815     


Thr Glu Asn Asp Met Leu Pro Asn Met Arg Ile Gly Ala Leu Gly Phe 
            820                 825                 830         


Ser Gly Ala Phe Glu Asp Arg Asp Pro Thr Gln Phe Glu Glu Arg His 
        835                 840                 845             


Leu Lys Phe Leu Gln Gln Leu Gly Lys Gly Asn Phe Gly Ser Val Glu 
    850                 855                 860                 


Met Cys Arg Tyr Asp Pro Leu Gln Asp Asn Thr Gly Glu Val Val Ala 
865                 870                 875                 880 


Val Lys Lys Leu Gln His Ser Thr Glu Glu His Leu Arg Asp Phe Glu 
                885                 890                 895     


Arg Glu Ile Glu Ile Leu Lys Ser Leu Gln His Asp Asn Ile Val Lys 
            900                 905                 910         


Tyr Lys Gly Val Cys Tyr Ser Ala Gly Arg Arg Asn Leu Lys Leu Ile 
        915                 920                 925             


Met Glu Tyr Leu Pro Tyr Gly Ser Leu Arg Asp Tyr Leu Gln Lys His 
    930                 935                 940                 


Lys Glu Arg Ile Asp His Ile Lys Leu Leu Gln Tyr Thr Ser Gln Ile 
945                 950                 955                 960 


Cys Lys Gly Met Glu Tyr Leu Gly Thr Lys Arg Tyr Ile His Arg Asp 
                965                 970                 975     


Leu Ala Thr Arg Asn Ile Leu Val Glu Asn Glu Asn Arg Val Lys Ile 
            980                 985                 990         


Gly Asp Phe Gly Leu Thr Lys Val  Leu Pro Gln Asp Lys  Glu Tyr Tyr 
        995                 1000                 1005             


Lys Val  Lys Glu Pro Gly Glu  Ser Pro Ile Phe Trp  Tyr Ala Pro 
    1010                 1015                 1020             


Glu Ser  Leu Thr Glu Ser Lys  Phe Ser Val Ala Ser  Asp Val Trp 
    1025                 1030                 1035             


Ser Phe  Gly Val Val Leu Tyr  Glu Leu Phe Thr Tyr  Ile Glu Lys 
    1040                 1045                 1050             


Ser Lys  Ser Pro Pro Ala Glu  Phe Met Arg Met Ile  Gly Asn Asp 
    1055                 1060                 1065             


Lys Gln  Gly Gln Met Ile Val  Phe His Leu Ile Glu  Leu Leu Lys 
    1070                 1075                 1080             


Asn Asn  Gly Arg Leu Pro Arg  Pro Asp Gly Cys Pro  Asp Glu Ile 
    1085                 1090                 1095             


Tyr Met  Ile Met Thr Glu Cys  Trp Asn Asn Asn Val  Asn Gln Arg 
    1100                 1105                 1110             


Pro Ser  Phe Arg Asp Leu Ala  Leu Arg Val Asp Gln  Ile Arg Asp 
    1115                 1120                 1125             


Asn Met  Ala Gly 
    1130         


<210> 3
<211> 128
<212> DNA
<213> Homo sapiens

<400> 3
ataaatcaaa ccttctagtc ttcagaacga atggtgtttc tgatgtacca acctcaccaa       60

cattacagag gcctactcat atgaaccaaa tggtgtttca caaaatcaga aatgaagatt      120

tgatattt                                                               128


<210> 4
<211> 135
<212> DNA
<213> Homo sapiens

<400> 4
aatgaaagcc ttggccaagg cacttttaca aagattttta aaggcgtacg aagagaagta       60

ggagactacg gtcaactgca tgaaacagaa gttcttttaa aagttctgga taaagcacac      120

agaaactatt cagag                                                       135


<210> 5
<211> 88
<212> DNA
<213> Homo sapiens

<400> 5
tctttctttg aagcagcaag tatgatgagc aagctttctc acaagcattt ggttttaaat       60

tatggagtat gtgtctgtgg agacgaga                                          88


<210> 6
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 6
tgtaaaacga cggccagtct aaatgctgtc ccccaaag                               38


<210> 7
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic primer

<400> 7
caggaaacag ctatgacccc atgccaactg tttagcaa                               38


<210> 8
<211> 11
<212> DNA
<213> Homo sapiens

<400> 8
cgtackaaga g                                                            11


<210> 9
<211> 11
<212> DNA
<213> Homo sapiens

<400> 9
actacrgtca a                                                            11


<210> 10
<211> 39
<212> DNA
<213> Homo sapiens

<400> 10
aaactattca gagwywttct kkkwmrcrrs wwkkwwraw                              39


<210> 11
<211> 14
<212> DNA
<213> Homo sapiens

<400> 11
atattcyggt tcag                                                         14


