                         SEQUENCE LISTING

<110>  New York Blood Center
       Jiang, Shibo
       Qi, Zhi
       Pan, Chungen
 
<120>  IMMUNOENHANCER-LINKED OLIGOMERIC HIV VACCINES

<130>  1958427-00142

<150>  US 61/106,101
<151>  2008-10-16

<160>  36    

<170>  PatentIn version 3.3

<210>  1
<211>  856
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  1

Met Arg Val Lys Glu Lys Tyr Gln His Leu Trp Arg Trp Gly Trp Arg 
1               5                   10                  15      


Trp Gly Thr Met Leu Leu Gly Met Leu Met Ile Cys Ser Ala Thr Glu 
            20                  25                  30          


Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Val Leu Val Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asp Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Ser 
        115                 120                 125             


Leu Lys Cys Thr Asp Leu Lys Asn Asp Thr Asn Thr Asn Ser Ser Ser 
    130                 135                 140                 


Gly Arg Met Ile Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Asn 
145                 150                 155                 160 


Ile Ser Thr Ser Ile Arg Gly Lys Val Gln Lys Glu Tyr Ala Phe Phe 
                165                 170                 175     


Tyr Lys Leu Asp Ile Ile Pro Ile Asp Asn Asp Thr Thr Ser Tyr Lys 
            180                 185                 190         


Leu Thr Ser Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Val 
        195                 200                 205             


Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe Ala 
    210                 215                 220                 


Ile Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Thr 
225                 230                 235                 240 


Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val Ser 
                245                 250                 255     


Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val Val Ile 
            260                 265                 270         


Arg Ser Val Asn Phe Thr Asp Asn Ala Lys Thr Ile Ile Val Gln Leu 
        275                 280                 285             


Asn Thr Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg 
    290                 295                 300                 


Lys Arg Ile Arg Ile Gln Arg Gly Pro Gly Arg Ala Phe Val Thr Ile 
305                 310                 315                 320 


Gly Lys Ile Gly Asn Met Arg Gln Ala His Cys Asn Ile Ser Arg Ala 
                325                 330                 335     


Lys Trp Asn Asn Thr Leu Lys Gln Ile Ala Ser Lys Leu Arg Glu Gln 
            340                 345                 350         


Phe Gly Asn Asn Lys Thr Ile Ile Phe Lys Gln Ser Ser Gly Gly Asp 
        355                 360                 365             


Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr 
    370                 375                 380                 


Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp 
385                 390                 395                 400 


Ser Thr Glu Gly Ser Asn Asn Thr Glu Gly Ser Asp Thr Ile Thr Leu 
                405                 410                 415     


Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Lys Val Gly Lys 
            420                 425                 430         


Ala Met Tyr Ala Pro Pro Ile Ser Gly Gln Ile Arg Cys Ser Ser Asn 
        435                 440                 445             


Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Ser Asn Asn Glu 
    450                 455                 460                 


Ser Glu Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg 
465                 470                 475                 480 


Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val 
                485                 490                 495     


Ala Pro Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Arg Ala 
            500                 505                 510         


Val Gly Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser 
        515                 520                 525             


Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Gln Leu 
    530                 535                 540                 


Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu 
545                 550                 555                 560 


Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu 
                565                 570                 575     


Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu 
            580                 585                 590         


Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val 
        595                 600                 605             


Pro Trp Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln Ile Trp Asn 
    610                 615                 620                 


His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser 
625                 630                 635                 640 


Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn 
                645                 650                 655     


Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp 
            660                 665                 670         


Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met Ile 
        675                 680                 685             


Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser Ile 
    690                 695                 700                 


Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser Phe Gln Thr His 
705                 710                 715                 720 


Leu Pro Thr Pro Arg Gly Pro Asp Arg Pro Glu Gly Ile Glu Glu Glu 
                725                 730                 735     


Gly Gly Glu Arg Asp Arg Asp Arg Ser Ile Arg Leu Val Asn Gly Ser 
            740                 745                 750         


Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys Leu Phe Ser Tyr 
        755                 760                 765             


His Arg Leu Arg Asp Leu Leu Leu Ile Val Thr Arg Ile Val Glu Leu 
    770                 775                 780                 


Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp Trp Asn Leu Leu 
785                 790                 795                 800 


Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val Ser Leu Leu Asn 
                805                 810                 815     


Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu Val 
            820                 825                 830         


Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro Arg Arg Ile Arg 
        835                 840                 845             


Gln Gly Leu Glu Arg Ile Leu Leu 
    850                 855     


<210>  2
<211>  345
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  2

Ala Val Gly Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly Ala Ala Gly 
1               5                   10                  15      


Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Gln 
            20                  25                  30          


Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile 
        35                  40                  45              


Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln 
    50                  55                  60                  


Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln 
65                  70                  75                  80  


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala 
                85                  90                  95      


Val Pro Trp Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln Ile Trp 
            100                 105                 110         


Asn His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr 
        115                 120                 125             


Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys 
    130                 135                 140                 


Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn 
145                 150                 155                 160 


Trp Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met 
                165                 170                 175     


Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser 
            180                 185                 190         


Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser Phe Gln Thr 
        195                 200                 205             


His Leu Pro Thr Pro Arg Gly Pro Asp Arg Pro Glu Gly Ile Glu Glu 
    210                 215                 220                 


Glu Gly Gly Glu Arg Asp Arg Asp Arg Ser Ile Arg Leu Val Asn Gly 
225                 230                 235                 240 


Ser Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys Leu Phe Ser 
                245                 250                 255     


Tyr His Arg Leu Arg Asp Leu Leu Leu Ile Val Thr Arg Ile Val Glu 
            260                 265                 270         


Leu Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp Trp Asn Leu 
        275                 280                 285             


Leu Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val Ser Leu Leu 
    290                 295                 300                 


Asn Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu 
305                 310                 315                 320 


Val Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro Arg Arg Ile 
                325                 330                 335     


Arg Gln Gly Leu Glu Arg Ile Leu Leu 
            340                 345 


<210>  3
<211>  45
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  3

Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile 
        35                  40                  45  


<210>  4
<211>  46
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  4

Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Lys 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu 
        35                  40                  45      


<210>  5
<211>  46
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  5

Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu 
        35                  40                  45      


<210>  6
<211>  46
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  6

Thr Leu Thr Ala Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val Leu 
        35                  40                  45      


<210>  7
<211>  46
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  7

Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln His 
1               5                   10                  15      


Gln Asn Asn Leu Leu Met Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu 
        35                  40                  45      


<210>  8
<211>  45
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  8

Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Gly Ile Val Gln Gln Gln 
1               5                   10                  15      


Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu 
            20                  25                  30          


Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu 
        35                  40                  45  


<210>  9
<211>  46
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  9

Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu 
        35                  40                  45      


<210>  10
<211>  46
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  10

Thr Leu Thr Val Gln Val Lys Lys Leu Leu Phe Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu 
        35                  40                  45      


<210>  11
<211>  46
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  11

Ala Leu Thr Val Arg Thr His Thr Leu Ile Lys Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Asp Asn Leu Leu Arg Ala Ile Gln Ala Gln Gln Gln Leu Leu Arg 
            20                  25                  30          


Leu Ser Val Trp Gly Ile Arg Gln Leu Arg Ala Arg Leu Leu 
        35                  40                  45      


<210>  12
<211>  46
<212>  PRT
<213>  Human immunodeficiency virus type 2

<400>  12

Thr Leu Ser Ala Gln Ser Arg Thr Leu Leu Ala Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Gln Gln Leu Leu Asp Val Val Lys Arg Gln Gln Glu Met Leu Arg 
            20                  25                  30          


Leu Thr Val Trp Gly Thr Lys Asn Leu Gln Ala Arg Val Thr 
        35                  40                  45      


<210>  13
<211>  17
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  13

Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile 
1               5                   10                  15      


Leu 
    


<210>  14
<211>  27
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  14

Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln 
1               5                   10                  15      


Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr 
            20                  25          


<210>  15
<211>  36
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  15

Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala 
1               5                   10                  15      


Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln 
            20                  25                  30          


Ala Arg Ile Leu 
        35      


<210>  16
<211>  51
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  16

Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu 
1               5                   10                  15      


Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp 
            20                  25                  30          


Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr Leu 
        35                  40                  45              


Lys Gln Gln 
    50      


<210>  17
<211>  63
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  17

Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Gln 
1               5                   10                  15      


Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile 
            20                  25                  30          


Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln 
        35                  40                  45              


Leu Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln 
    50                  55                  60              


<210>  18
<211>  27
<212>  PRT
<213>  Bacteriophage T4

<400>  18

Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
1               5                   10                  15      


Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
            20                  25          


<210>  19
<211>  28
<212>  PRT
<213>  Human immunodeficiency virus

<400>  19

Arg Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Glu Ser Lys Gln 
1               5                   10                  15      


Lys Lys Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys 
            20                  25              


<210>  20
<211>  26
<212>  PRT
<213>  Human immunodeficiency virus

<400>  20

Ile Lys Lys Glu Ile Glu Ala Ile Lys Lys Glu Gln Glu Ala Ile Lys 
1               5                   10                  15      


Lys Lys Ile Glu Ala Ile Glu Lys Glu Ile 
            20                  25      


<210>  21
<211>  229
<212>  PRT
<213>  Homo sapiens

<400>  21

Arg Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            100                 105                 110         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Gly Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Pro Gly Lys 
225                 


<210>  22
<211>  234
<212>  PRT
<213>  Mus sp.

<400>  22

Arg Ser Pro Arg Gly Pro Thr Ile Lys Pro Cys Pro Pro Cys Lys Cys 
1               5                   10                  15      


Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro 
            20                  25                  30          


Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser Pro Ile Val Thr Cys 
        35                  40                  45              


Val Val Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln Ile Ser Trp 
    50                  55                  60                  


Phe Val Asn Asn Val Glu Val His Thr Ala Gln Thr Gln Thr His Arg 
65                  70                  75                  80  


Glu Asp Tyr Asn Ser Thr Leu Arg Val Val Ser Ala Leu Pro Ile Gln 
                85                  90                  95      


His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn 
            100                 105                 110         


Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile Ser Lys Pro Lys Gly 
        115                 120                 125             


Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro Pro Pro Glu Glu Glu 
    130                 135                 140                 


Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met Val Thr Asp Phe Met 
145                 150                 155                 160 


Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn Gly Lys Thr Glu Leu 
                165                 170                 175     


Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe 
            180                 185                 190         


Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn Trp Val Glu Arg Asn 
        195                 200                 205             


Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu His Asn His His Thr 
    210                 215                 220                 


Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys 
225                 230                 


<210>  23
<211>  225
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  23

Arg Ser Ser Lys Pro Thr Cys Pro Pro Pro Glu Leu Leu Gly Gly Pro 
1               5                   10                  15      


Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
            20                  25                  30          


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Asp Asp 
        35                  40                  45              


Pro Glu Val Gln Phe Thr Trp Tyr Ile Asn Asn Glu Gln Val Arg Thr 
    50                  55                  60                  


Ala Arg Pro Pro Leu Arg Glu Gln Gln Phe Asn Ser Thr Ile Arg Val 
65                  70                  75                  80  


Val Ser Thr Leu Pro Ile Ala His Gln Asp Trp Leu Arg Gly Lys Glu 
                85                  90                  95      


Phe Lys Cys Lys Val His Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
            100                 105                 110         


Thr Ile Ser Lys Ala Arg Gly Gln Pro Leu Glu Pro Lys Val Tyr Thr 
        115                 120                 125             


Met Gly Pro Pro Arg Glu Glu Leu Ser Ser Arg Ser Val Ser Leu Thr 
    130                 135                 140                 


Cys Met Ile Asn Gly Phe Tyr Pro Ser Asp Ile Ser Val Glu Trp Glu 
145                 150                 155                 160 


Lys Asn Gly Lys Ala Glu Asp Asn Tyr Lys Thr Thr Pro Ala Val Leu 
                165                 170                 175     


Asp Ser Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val Pro Thr 
            180                 185                 190         


Ser Glu Trp Gln Arg Gly Asp Val Phe Thr Cys Ser Val Met His Glu 
        195                 200                 205             


Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser Arg Ser Pro Gly 
    210                 215                 220                 


Lys 
225 


<210>  24
<211>  73
<212>  PRT
<213>  Artificial

<220>
<223>  N46Fd fusion protein comprising N46 fragment of gp41 and foldon 
       sequnce

<400>  24

Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Gly Tyr 
        35                  40                  45              


Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly 
    50                  55                  60                  


Glu Trp Val Leu Leu Ser Thr Phe Leu 
65                  70              


<210>  25
<211>  275
<212>  PRT
<213>  Artificial

<220>
<223>  N46hFc fusion protein comprising the N46 fragment of gp41 and 
       human Fc

<400>  25

Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Arg Ser 
        35                  40                  45              


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
    50                  55                  60                  


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
65                  70                  75                  80  


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
                85                  90                  95      


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
            100                 105                 110         


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
        115                 120                 125             


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
    130                 135                 140                 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
145                 150                 155                 160 


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
                165                 170                 175     


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
            180                 185                 190         


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
        195                 200                 205             


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
    210                 215                 220                 


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
225                 230                 235                 240 


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
                245                 250                 255     


His Glu Gly Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
            260                 265                 270         


Pro Gly Lys 
        275 


<210>  26
<211>  302
<212>  PRT
<213>  Artificial

<220>
<223>  N46FdhFc fusion protein comprising the N46 fragment of gp41, 
       foldon sequence and human Fc

<400>  26

Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Gly Tyr 
        35                  40                  45              


Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly 
    50                  55                  60                  


Glu Trp Val Leu Leu Ser Thr Phe Leu Arg Ser Asp Lys Thr His Thr 
65                  70                  75                  80  


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
                85                  90                  95      


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            100                 105                 110         


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
        115                 120                 125             


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    130                 135                 140                 


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
145                 150                 155                 160 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                165                 170                 175     


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            180                 185                 190         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
        195                 200                 205             


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
    210                 215                 220                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
225                 230                 235                 240 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                245                 250                 255     


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
            260                 265                 270         


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Gly Leu His 
        275                 280                 285             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    290                 295                 300         


<210>  27
<211>  302
<212>  PRT
<213>  Artificial

<220>
<223>  N46FdmFc comprising the N46 fragment of gp41, foldon sequence and
       murine Fc

<400>  27

Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Gly Tyr 
        35                  40                  45              


Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly 
    50                  55                  60                  


Glu Trp Val Leu Leu Ser Thr Phe Leu Arg Ser Asp Lys Thr His Thr 
65                  70                  75                  80  


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
                85                  90                  95      


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            100                 105                 110         


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
        115                 120                 125             


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    130                 135                 140                 


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
145                 150                 155                 160 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                165                 170                 175     


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            180                 185                 190         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
        195                 200                 205             


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
    210                 215                 220                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
225                 230                 235                 240 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                245                 250                 255     


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
            260                 265                 270         


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Gly Leu His 
        275                 280                 285             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    290                 295                 300         


<210>  28
<211>  302
<212>  PRT
<213>  Artificial

<220>
<223>  N46FdrFc comprising N46 sequence of gp41, foldon sequence and 
       rabbit Fc

<400>  28

Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
1               5                   10                  15      


Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
            20                  25                  30          


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Gly Tyr 
        35                  40                  45              


Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly 
    50                  55                  60                  


Glu Trp Val Leu Leu Ser Thr Phe Leu Arg Ser Asp Lys Thr His Thr 
65                  70                  75                  80  


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
                85                  90                  95      


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            100                 105                 110         


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
        115                 120                 125             


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    130                 135                 140                 


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
145                 150                 155                 160 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                165                 170                 175     


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            180                 185                 190         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
        195                 200                 205             


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
    210                 215                 220                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
225                 230                 235                 240 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                245                 250                 255     


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
            260                 265                 270         


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Gly Leu His 
        275                 280                 285             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    290                 295                 300         


<210>  29
<211>  74
<212>  PRT
<213>  Artificial

<220>
<223>  IQN46 fusion protein comprising N46 sequence of gp41 and IQ 
       sequence

<400>  29

Arg Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Glu Ser Lys Gln 
1               5                   10                  15      


Lys Lys Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys Thr Leu Thr Val 
            20                  25                  30          


Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu 
        35                  40                  45              


Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp 
    50                  55                  60                  


Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu 
65                  70                  


<210>  30
<211>  303
<212>  PRT
<213>  Artificial

<220>
<223>  IQN46hFc fusion protein comprising N46 sequence of gp41, IQ 
       sequence and human Fc

<400>  30

Arg Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Glu Ser Lys Gln 
1               5                   10                  15      


Lys Lys Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys Thr Leu Thr Val 
            20                  25                  30          


Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu 
        35                  40                  45              


Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp 
    50                  55                  60                  


Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Arg Ser Asp Lys Thr His 
65                  70                  75                  80  


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
                85                  90                  95      


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
            100                 105                 110         


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
        115                 120                 125             


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
    130                 135                 140                 


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
145                 150                 155                 160 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
                165                 170                 175     


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
            180                 185                 190         


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
        195                 200                 205             


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
    210                 215                 220                 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
225                 230                 235                 240 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
                245                 250                 255     


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
            260                 265                 270         


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Gly Leu 
        275                 280                 285             


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    290                 295                 300             


<210>  31
<211>  72
<212>  PRT
<213>  Artificial

<220>
<223>  IZN46 fusion protein comprising the N46 sequence of gp41 and IZ 
       sequence

<400>  31

Ile Lys Lys Glu Ile Glu Ala Ile Lys Lys Glu Gln Glu Ala Ile Lys 
1               5                   10                  15      


Lys Lys Ile Glu Ala Ile Glu Lys Glu Ile Thr Leu Thr Val Gln Ala 
            20                  25                  30          


Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg 
        35                  40                  45              


Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile 
    50                  55                  60                  


Lys Gln Leu Gln Ala Arg Ile Leu 
65                  70          


<210>  32
<211>  301
<212>  PRT
<213>  Artificial

<220>
<223>  IZN46hFc fusion protein comprising the N46 sequence of gp41, IZ 
       sequence and human Fc

<400>  32

Ile Lys Lys Glu Ile Glu Ala Ile Lys Lys Glu Gln Glu Ala Ile Lys 
1               5                   10                  15      


Lys Lys Ile Glu Ala Ile Glu Lys Glu Ile Thr Leu Thr Val Gln Ala 
            20                  25                  30          


Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg 
        35                  40                  45              


Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile 
    50                  55                  60                  


Lys Gln Leu Gln Ala Arg Ile Leu Arg Ser Asp Lys Thr His Thr Cys 
65                  70                  75                  80  


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
                85                  90                  95      


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
            100                 105                 110         


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
        115                 120                 125             


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
    130                 135                 140                 


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
145                 150                 155                 160 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
                165                 170                 175     


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
            180                 185                 190         


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
        195                 200                 205             


Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
    210                 215                 220                 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
225                 230                 235                 240 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
                245                 250                 255     


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
            260                 265                 270         


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Gly Leu His Asn 
        275                 280                 285             


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    290                 295                 300     


<210>  33
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  N46Fd forward primer

<400>  33
ccggaattcg acgctgacgg tacagg                                          26


<210>  34
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  N46Fd reverse primer

<400>  34
ggaagatctt cagtggtggt ggtggtggtg cagaaaggta ga                        42


<210>  35
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  N46FdhFc reverse primer

<400>  35
ggaagatctc agaaaggtag acag                                            24


<210>  36
<211>  81
<212>  DNA
<213>  Bacteriophage T4

<400>  36
ggctatattc cggaagcgcc gcgtgatggc caggcgtatg tgcgtaaaga tggcgaatgg     60

gtgctgctgt ctacctttct g                                               81


