SEQUNECE LISTING

<110> The Regents of The University of California

<120> Selection of HIV vaccine antigens by use of intrapatient sequence variation to identify mutations in the HIV envelope glycoprotein that affect the binding of broadly neutralizing antibodies

<130> UCSC2008-776 PCT

<150> US 61/195,112
<151> 2008-10-04

<160> 5

<210> 1
<211> 854
<212> PRT
<213> Homo sapiens

<400> 1
Met Lys Val Lys Gly Ile Lys Lys Ser Cys Gln His Leu Trp Lys 
  1               5                  10                  15

Trp Gly Ile Leu Leu Leu Gly Met Leu Met Ile Cys Ser Ala Ala 
                 20                  25                  30

Glu Lys Met Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
                 35                  40                  45

Glu Ala Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ser Tyr 
                 50                  55                  60

Asp Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro 
                 65                  70                  75

Thr Asp Pro Asn Pro Gln Glu Val Val Leu Gly Asn Val Thr Glu 
                 80                  85                  90

Asn Phe Asn Met Trp Lys Asn Asn Met Val Glu Gln Met His Glu 
                 95                 100                 105

Asp Val Ile Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys 
                110                 115                 120

Leu Thr Pro Leu Cys Val Thr Leu Asn Cys Thr Asp Lys Leu Arg 
                125                 130                 135

Asn Asp Ala Phe Gly Val Asn Asn Thr Met Glu Gly Glu Met Lys 
                140                 145                 150

Asn Cys Ser Phe Asn Thr Thr Thr Ser Leu Arg Asp Lys Ile Gln 
                155                 160                 165

Lys Glu Tyr Ala Leu Phe Tyr Lys Leu Asp Val Val Gln Ile Lys 
                170                 175                 180

Asn Asn Asn Asn Ser Asn Tyr Thr Ser Tyr Arg Leu Ile Asn Cys 
                185                 190                 195

Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Val Thr Phe Glu 
                200                 205                 210

Pro Ile Pro Ile His Tyr Cys Thr Pro Ala Gly Phe Ala Ile Leu 
                215                 220                 225

Lys Cys Asn Asn Lys Thr Phe Ser Gly Lys Gly Thr Cys Thr Asn 
                230                 235                 240

Val Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val Ser 
                245                 250                 255

Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Asp Val Val 
                260                 265                 270

Ile Arg Ser Asp Asn Phe Ser Gln Asn Ala Lys Ile Ile Ile Val 
                275                 280                 285

Gln Leu Asn Glu Ala Val Val Ile Asn Cys Thr Arg Pro Gly Asn 
                290                 295                 300

Asn Thr Arg Lys Ser Ile Pro Ile Gly Pro Gly Arg Ala Phe Tyr 
                305                 310                 315

Ala Thr Gly Asp Ile Ile Gly Asn Ile Arg Gln Ala His Cys Asn 
                320                 325                 330

Val Ser Ser Thr Lys Trp Asn Asn Thr Leu Gln Lys Ile Val Glu 
                335                 340                 345

Lys Leu Arg Glu Gln Phe Gly Asn Lys Thr Ile Lys Phe Thr Ser 
                350                 355                 360

Pro Ser Pro Gly Gly Asp Pro Glu Ile Val Met His Ser Phe Asn 
                365                 370                 375

Cys Gly Gly Glu Phe Phe Tyr Cys Asp Thr Thr Gln Leu Phe Asn 
                380                 385                 390

Ser Thr Trp Asp Asn Thr Ser Thr Trp Asn Asn Ser Asn Thr Gln 
                395                 400                 405

Asn Lys Asn Asp Arg Asn Ile Thr Leu Gln Cys Arg Ile Lys Gln 
                410                 415                 420

Ile Ile Asn Met Trp Gln Glu Val Gly Lys Ala Met Tyr Ala Pro 
                425                 430                 435

Pro Ile Met Gly Gln Ile Arg Cys Val Ser Asn Ile Thr Gly Leu 
                440                 445                 450

Leu Leu Thr Arg Asp Gly Gly Asn Gly Ser Glu Ala Lys Asn Asp 
                455                 460                 465

Thr Glu Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp 
                470                 475                 480

Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu 
                485                 490                 495

Gly Val Ala Pro Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu 
                500                 505                 510

Lys Arg Ala Val Gly Thr Ile Gly Ala Met Phe Leu Gly Phe Leu 
                515                 520                 525

Gly Ala Ala Gly Asn Thr Met Gly Ala Ala Ser Leu Thr Leu Thr 
                530                 535                 540

Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn 
                545                 550                 555

Asn Leu Leu Arg Ala Ile Gln Ala Gln Gln His Leu Leu Gln Leu 
                560                 565                 570

Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val 
                575                 580                 585

Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys 
                590                 595                 600

Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp Asn Ala Ser 
                605                 610                 615

Trp Ser Asn Lys Ser Tyr Thr Asp Ile Trp Asp Asn Met Thr Trp 
                620                 625                 630

Met Gln Trp Glu Lys Glu Ile Glu Asn Tyr Thr Ser Leu Ile Tyr 
                635                 640                 645

Thr Leu Ile Glu Asp Ser Gln Asn Gln Arg Glu Lys Asn Glu Gln 
                650                 655                 660

Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp Phe 
                665                 670                 675

Asp Ile Thr Ser Trp Leu Trp Tyr Ile Lys Ile Phe Ile Met Ile 
                680                 685                 690

Val Gly Gly Leu Ile Gly Leu Arg Ile Val Phe Ala Val Leu Ser 
                695                 700                 705

Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser Leu Gln 
                710                 715                 720

Thr Arg Leu Pro Ala Pro Gly Gly Pro Asp Arg Pro Gly Gly Ile 
                725                 730                 735

Glu Glu Glu Gly Gly Glu Gln Gly Arg Gly Arg Ser Val Arg Leu 
                740                 745                 750

Val Asp Gly Phe Leu Ala Leu Ile Trp Asp Asp Leu Arg Asn Leu 
                755                 760                 765

Cys Leu Phe Ile Tyr His Arg Leu Arg Asp Leu Leu Trp Ile Val 
                770                 775                 780

Gly Leu Leu Gly Arg Arg Gly Trp Glu Ile Leu Lys Tyr Trp Trp 
                785                 790                 795

Asn Ile Leu Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val 
                800                 805                 810

Ser Leu Leu Asn Thr Ile Ala Ile Ala Val Ala Glu Gly Thr Asp 
                815                 820                 825

Arg Ile Ile Glu Leu Ala Gln Gly Ile Cys Arg Ala Ile Leu His 
                830                 835                 840

Ile Pro Arg Arg Ile Arg Gln Gly Phe Glu Arg Ala Leu Leu
                845                 850                  


<210> 2
<211> 685
<212> PRT
<213> Homo sapiens
<400> 2
Met Lys Val Lys Gly Ile Lys Lys Ser Cys Gln His Leu Trp Lys 
  1               5                  10                  15

Trp Gly Ile Leu Leu Leu Gly Met Leu Met Ile Cys Ser Ala Ala 
                 20                  25                  30

Glu Lys Met Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
                 35                  40                  45

Glu Ala Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ser Tyr 
                 50                  55                  60

Asp Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro 
                 65                  70                  75

Thr Asp Pro Asn Pro Gln Glu Val Val Leu Gly Asn Val Thr Glu 
                 80                  85                  90

Asn Phe Asn Met Trp Lys Asn Asn Met Val Glu Gln Met His Glu 
                 95                 100                 105

Asp Val Ile Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys 
                110                 115                 120

Leu Thr Pro Leu Cys Val Thr Leu Asn Cys Thr Asp Lys Leu Arg 
                125                 130                 135

Asn Asp Ala Phe Gly Val Asn Asn Thr Met Glu Gly Glu Met Lys 
                140                 145                 150

Asn Cys Ser Phe Asn Thr Thr Thr Ser Leu Arg Asp Lys Ile Gln 
                155                 160                 165

Lys Glu Tyr Ala Leu Phe Tyr Lys Leu Asp Val Val Gln Ile Lys 
                170                 175                 180

Asn Asn Asn Asn Ser Asn Tyr Thr Ser Tyr Arg Leu Ile Asn Cys 
                185                 190                 195

Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Val Thr Phe Glu 
                200                 205                 210

Pro Ile Pro Ile His Tyr Cys Thr Pro Ala Gly Phe Ala Ile Leu 
                215                 220                 225

Lys Cys Asn Asn Lys Thr Phe Ser Gly Lys Gly Thr Cys Thr Asn 
                230                 235                 240

Val Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val Ser 
                245                 250                 255

Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Asp Val Val 
                260                 265                 270

Ile Arg Ser Asp Asn Phe Ser Gln Asn Ala Lys Ile Ile Ile Val 
                275                 280                 285

Gln Leu Asn Glu Ala Val Val Ile Asn Cys Thr Arg Pro Gly Asn 
                290                 295                 300

Asn Thr Arg Lys Ser Ile Pro Ile Gly Pro Gly Arg Ala Phe Tyr 
                305                 310                 315

Ala Thr Gly Asp Ile Ile Gly Asn Ile Arg Gln Ala His Cys Asn 
                320                 325                 330

Val Ser Ser Thr Lys Trp Asn Asn Thr Leu Gln Lys Ile Val Glu 
                335                 340                 345

Lys Leu Arg Glu Gln Phe Gly Asn Lys Thr Ile Lys Phe Thr Ser 
                350                 355                 360

Pro Ser Pro Gly Gly Asp Pro Glu Ile Val Met His Ser Phe Asn 
                365                 370                 375

Cys Gly Gly Glu Phe Phe Tyr Cys Asp Thr Thr Gln Leu Phe Asn 
                380                 385                 390

Ser Thr Trp Asp Asn Thr Ser Thr Trp Asn Asn Ser Asn Thr Gln 
                395                 400                 405

Asn Lys Asn Asp Arg Asn Ile Thr Leu Gln Cys Arg Ile Lys Gln 
                410                 415                 420

Ile Ile Asn Met Trp Gln Glu Val Gly Lys Ala Met Tyr Ala Pro 
                425                 430                 435

Pro Ile Met Gly Gln Ile Arg Cys Val Ser Asn Ile Thr Gly Leu 
                440                 445                 450

Leu Leu Thr Arg Asp Gly Gly Asn Gly Ser Glu Ala Lys Asn Asp 
                455                 460                 465

Thr Glu Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp 
                470                 475                 480

Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu 
                485                 490                 495

Gly Val Ala Pro Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu 
                500                 505                 510

Lys Arg Ala Val Gly Thr Ile Gly Ala Met Phe Leu Gly Phe Leu 
                515                 520                 525

Gly Ala Ala Gly Asn Thr Met Gly Ala Ala Ser Leu Thr Leu Thr 
                530                 535                 540

Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Asn 
                545                 550                 555

Asn Leu Leu Arg Ala Ile Gln Ala Gln Gln His Leu Leu Gln Leu 
                560                 565                 570

Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val 
                575                 580                 585

Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys 
                590                 595                 600

Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp Asn Ala Ser 
                605                 610                 615

Trp Ser Asn Lys Ser Tyr Thr Asp Ile Trp Asp Asn Met Thr Trp 
                620                 625                 630

Met Gln Trp Glu Lys Glu Ile Glu Asn Tyr Thr Ser Leu Ile Tyr 
                635                 640                 645

Thr Leu Ile Glu Asp Ser Gln Asn Gln Arg Glu Lys Asn Glu Gln 
                650                 655                 660

Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp Phe 
                665                 670                 675

Asp Ile Thr Ser Trp Leu Trp Tyr Ile Lys 
                680                 685


<210> 3
<211> 645
<212> PRT
<213> Artificial Sequence

<400> 3
Val Pro Val Trp Lys Glu Ala Thr Thr Thr Leu Phe Cys Ala Ser 
  1               5                  10                  15

Asp Ala Lys Ser Tyr Asp Thr Glu Val His Asn Val Trp Ala Thr 
                 20                  25                  30

His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Val Val Leu 
                 35                  40                  45

Gly Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn Asn Met Val 
                 50                  55                  60

Glu Gln Met His Glu Asp Val Ile Ser Leu Trp Asp Gln Ser Leu 
                 65                  70                  75

Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn Cys 
                 80                  85                  90

Thr Asp Lys Leu Arg Asn Asp Ala Phe Gly Val Asn Asn Thr Met 
                 95                 100                 105

Glu Gly Glu Met Lys Asn Cys Ser Phe Asn Thr Thr Thr Ser Leu 
                110                 115                 120

Arg Asp Lys Ile Gln Lys Glu Tyr Ala Leu Phe Tyr Lys Leu Asp 
                125                 130                 135

Val Val Gln Ile Lys Asn Asn Asn Asn Ser Asn Tyr Thr Ser Tyr 
                140                 145                 150

Arg Leu Ile Asn Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro 
                155                 160                 165

Lys Val Thr Phe Glu Pro Ile Pro Ile His Tyr Cys Thr Pro Ala 
                170                 175                 180

Gly Phe Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Ser Gly Lys 
                185                 190                 195

Gly Thr Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile 
                200                 205                 210

Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala 
                215                 220                 225

Glu Glu Asp Val Val Ile Arg Ser Asp Asn Phe Ser Gln Asn Ala 
                230                 235                 240

Lys Ile Ile Ile Val Gln Leu Asn Glu Ala Val Val Ile Asn Cys 
                245                 250                 255

Thr Arg Pro Gly Asn Asn Thr Arg Lys Ser Ile Pro Ile Gly Pro 
                260                 265                 270

Gly Arg Ala Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asn Ile Arg 
                275                 280                 285

Gln Ala His Cys Asn Val Ser Ser Thr Lys Trp Asn Asn Thr Leu 
                290                 295                 300

Gln Lys Ile Val Glu Lys Leu Arg Glu Gln Phe Gly Asn Lys Thr 
                305                 310                 315

Ile Lys Phe Thr Ser Pro Ser Pro Gly Gly Asp Pro Glu Ile Val 
                320                 325                 330

Met His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asp Thr 
                335                 340                 345

Thr Gln Leu Phe Asn Ser Thr Trp Asp Asn Thr Ser Thr Trp Asn 
                350                 355                 360

Asn Ser Asn Thr Gln Asn Lys Asn Asp Arg Asn Ile Thr Leu Gln 
                365                 370                 375

Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Lys 
                380                 385                 390

Ala Met Tyr Ala Pro Pro Ile Met Gly Gln Ile Arg Cys Val Ser 
                395                 400                 405

Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Gly Ser 
                410                 415                 420

Glu Ala Lys Asn Asp Thr Glu Ile Phe Arg Pro Gly Gly Gly Asp 
                425                 430                 435

Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val 
                440                 445                 450

Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg Arg 
                455                 460                 465

Val Val Gln Arg Glu Lys Arg Ala Val Gly Thr Ile Gly Ala Met 
                470                 475                 480

Phe Leu Gly Phe Leu Gly Ala Ala Gly Asn Thr Met Gly Ala Ala 
                485                 490                 495

Ser Leu Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile 
                500                 505                 510

Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Gln Ala Gln Gln 
                515                 520                 525

His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala 
                530                 535                 540

Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu 
                545                 550                 555

Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val 
                560                 565                 570

Pro Trp Asn Ala Ser Trp Ser Asn Lys Ser Tyr Thr Asp Ile Trp 
                575                 580                 585

Asp Asn Met Thr Trp Met Gln Trp Glu Lys Glu Ile Glu Asn Tyr 
                590                 595                 600

Thr Ser Leu Ile Tyr Thr Leu Ile Glu Asp Ser Gln Asn Gln Arg 
                605                 610                 615

Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser 
                620                 625                 630

Leu Trp Asn Trp Phe Asp Ile Thr Ser Trp Leu Trp Tyr Ile Lys
                635                 640                 645


<210> 4
<211> 645
<212> PRT
<213> Artificial Sequence

<400> 4
Val Pro Val Trp Lys Glu Ala Thr Thr Thr Leu Phe Cys Ala Ser 
  1               5                  10                  15

Asp Ala Lys Ser Tyr Asp Thr Glu Val His Asn Val Trp Ala Thr 
                 20                  25                  30

His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Val Val Leu 
                 35                  40                  45

Gly Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn Asn Met Val 
                 50                  55                  60

Glu Gln Met His Glu Asp Val Ile Ser Leu Trp Asp Gln Ser Leu 
                 65                  70                  75

Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn Cys 
                 80                  85                  90

Thr Asp Lys Leu Arg Asn Asp Ala Phe Gly Val Asn Asn Thr Met 
                 95                 100                 105

Glu Gly Glu Met Lys Asn Cys Ser Phe Asn Thr Thr Thr Ser Leu 
                110                 115                 120

Arg Asp Lys Ile Gln Lys Glu Tyr Ala Leu Phe Tyr Lys Leu Asp 
                125                 130                 135

Val Val Gln Ile Lys Asn Asn Asn Asn Ser Asn Tyr Thr Ser Tyr 
                140                 145                 150

Arg Leu Ile Asn Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro 
                155                 160                 165

Lys Val Thr Phe Glu Pro Ile Pro Ile His Tyr Cys Thr Pro Ala 
                170                 175                 180

Gly Phe Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Ser Gly Lys 
                185                 190                 195

Gly Thr Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile 
                200                 205                 210

Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala 
                215                 220                 225

Glu Glu Asp Val Val Ile Arg Ser Asp Asn Phe Ser Gln Asn Ala 
                230                 235                 240

Lys Ile Ile Ile Val Gln Leu Asn Glu Ala Val Val Ile Asn Cys 
                245                 250                 255

Thr Arg Pro Gly Asn Asn Thr Arg Lys Ser Ile Pro Ile Gly Pro 
                260                 265                 270

Gly Arg Ala Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asn Ile Arg 
                275                 280                 285

Gln Ala His Cys Asn Val Ser Ser Thr Lys Trp Asn Asn Thr Leu 
                290                 295                 300

Gln Lys Ile Val Glu Lys Leu Arg Glu Gln Phe Gly Asn Lys Thr 
                305                 310                 315

Ile Lys Phe Thr Ser Pro Ser Pro Gly Gly Asp Pro Glu Ile Val 
                320                 325                 330

Met His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asp Thr 
                335                 340                 345

Thr Gln Leu Phe Asn Ser Thr Trp Asp Asn Thr Ser Thr Trp Asn 
                350                 355                 360

Asn Ser Asn Thr Gln Asn Lys Asn Asp Arg Asn Ile Thr Leu Gln 
                365                 370                 375

Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Lys 
                380                 385                 390

Ala Met Tyr Ala Pro Pro Ile Met Gly Gln Ile Arg Cys Val Ser 
                395                 400                 405

Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Gly Ser 
                410                 415                 420

Glu Ala Lys Asn Asp Thr Glu Ile Phe Arg Pro Gly Gly Gly Asp 
                425                 430                 435

Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val 
                440                 445                 450

Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg Arg 
                455                 460                 465

Val Val Gln Gly Glu Gly Gly Ala Val Gly Thr Ile Gly Ala Met 
                470                 475                 480

Phe Leu Gly Phe Leu Gly Ala Ala Gly Asn Thr Met Gly Ala Ala 
                485                 490                 495

Ser Leu Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile 
                500                 505                 510

Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Gln Ala Gln Gln 
                515                 520                 525

His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala 
                530                 535                 540

Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu 
                545                 550                 555

Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val 
                560                 565                 570

Pro Trp Asn Ala Ser Trp Ser Asn Lys Ser Tyr Thr Asp Ile Trp 
                575                 580                 585

Asp Asn Met Thr Trp Met Gln Trp Glu Lys Glu Ile Glu Asn Tyr 
                590                 595                 600

Thr Ser Leu Ile Tyr Thr Leu Ile Glu Asp Ser Gln Asn Gln Arg 
                605                 610                 615

Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser 
                620                 625                 630

Leu Trp Asn Trp Phe Asp Ile Thr Ser Trp Leu Trp Tyr Ile Lys
                635                 640                 645


<210> 5
<211> 645
<212> PRT
<213> Artificial Sequence
<400> 5
Val Pro Val Trp Lys Glu Ala Thr Thr Thr Leu Phe Cys Ala Ser 
  1               5                  10                  15

Asp Ala Lys Ser Tyr Asp Thr Glu Val His Asn Val Trp Ala Thr 
                 20                  25                  30

His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Val Val Leu 
                 35                  40                  45

Gly Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn Asn Met Val 
                 50                  55                  60

Glu Gln Met His Glu Asp Val Ile Ser Leu Trp Asp Gln Ser Leu 
                 65                  70                  75

Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn Cys 
                 80                  85                  90

Thr Asp Lys Leu Arg Asn Asp Ala Phe Gly Val Asn Asn Thr Met 
                 95                 100                 105

Glu Gly Glu Met Lys Asn Cys Ser Phe Asn Thr Thr Thr Ser Leu 
                110                 115                 120

Arg Asp Lys Ile Gln Lys Glu Tyr Ala Leu Phe Tyr Lys Leu Asp 
                125                 130                 135

Val Val Gln Ile Lys Asn Asn Asn Asn Ser Asn Tyr Thr Ser Tyr 
                140                 145                 150

Arg Leu Ile Asn Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro 
                155                 160                 165

Lys Val Thr Phe Glu Pro Ile Pro Ile His Tyr Cys Thr Pro Ala 
                170                 175                 180

Gly Phe Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Ser Gly Lys 
                185                 190                 195

Gly Thr Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile 
                200                 205                 210

Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala 
                215                 220                 225

Glu Glu Asp Val Val Ile Arg Ser Asp Asn Phe Ser Gln Asn Ala 
                230                 235                 240

Lys Ile Ile Ile Val Gln Leu Asn Glu Ala Val Val Ile Asn Cys 
                245                 250                 255

Thr Arg Pro Gly Asn Asn Thr Arg Lys Ser Ile Pro Ile Gly Pro 
                260                 265                 270

Gly Arg Ala Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asn Ile Arg 
                275                 280                 285

Gln Ala His Cys Asn Val Ser Ser Thr Lys Trp Asn Asn Thr Leu 
                290                 295                 300

Gln Lys Ile Val Glu Lys Leu Arg Glu Gln Phe Gly Asn Lys Thr 
                305                 310                 315

Ile Lys Phe Thr Ser Pro Ser Pro Gly Gly Asp Pro Glu Ile Val 
                320                 325                 330

Met His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asp Thr 
                335                 340                 345

Thr Gln Leu Phe Asn Ser Thr Trp Asp Asn Thr Ser Thr Trp Asn 
                350                 355                 360

Asn Ser Asn Thr Gln Asn Lys Asn Asp Arg Asn Ile Thr Leu Gln 
                365                 370                 375

Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Lys 
                380                 385                 390

Ala Met Tyr Ala Pro Pro Ile Met Gly Gln Ile Arg Cys Val Ser 
                395                 400                 405

Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Gly Ser 
                410                 415                 420

Glu Ala Lys Asn Asp Thr Glu Ile Phe Arg Pro Gly Gly Gly Asp 
                425                 430                 435

Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val 
                440                 445                 450

Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg Arg 
                455                 460                 465

Val Val Gln Arg Glu Lys Arg Ala Val Gly Thr Ile Gly Ala Met 
                470                 475                 480

Phe Leu Gly Phe Leu Gly Ala Ala Gly Asn Thr Met Gly Ala Ala 
                485                 490                 495

Ser Leu Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile 
                500                 505                 510

Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Gln Ala Gln Gln 
                515                 520                 525

His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala 
                530                 535                 540

Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu 
                545                 550                 555

Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val 
                560                 565                 570

Pro Trp Asn Ala Ser Trp Ser Asn Lys Ser Tyr Thr Asp Ile Trp 
                575                 580                 585

Asp Asn Met Thr Trp Met Gln Trp Glu Lys Glu Ile Glu Asn Tyr 
                590                 595                 600

Thr Ser Leu Ile Tyr Thr Leu Ile Glu Asp Ser Gln Asn Gln Arg 
                605                 610                 615

Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser 
                620                 625                 630

Leu Trp Asn Trp Phe Asp Ile Thr Ser Trp Leu Trp Tyr Ile Lys
                635                 640                 645


