
                                SEQUENCE LISTING

<110> THE J. DAVID GLADSTONE INSTITUTES
      Ott, Melanie
      Herker, Eva
      Farese, Robert V.
      Harris, Charles

<120> Methods of Treating Hepatitis C Virus
  Infection

<130> GLAD-050WO

<150> US 61/102,250       
<151> 2008-10-02  

<160> 19

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 488
<212> PRT
<213> Homo sapiens

<400> 1
Met Gly Asp Arg Gly Ser Ser Arg Arg Arg Arg Thr Gly Ser Arg Pro
 1               5                  10                  15      
Ser Ser His Gly Gly Gly Gly Pro Ala Ala Ala Glu Glu Glu Val Arg
            20                  25                  30          
Asp Ala Ala Ala Gly Pro Asp Val Gly Ala Ala Gly Asp Ala Pro Ala
        35                  40                  45              
Pro Ala Pro Asn Lys Asp Gly Asp Ala Gly Val Gly Ser Gly His Trp
    50                  55                  60                  
Glu Leu Arg Cys His Arg Leu Gln Asp Ser Leu Phe Ser Ser Asp Ser
65                  70                  75                  80  
Gly Phe Ser Asn Tyr Arg Gly Ile Leu Asn Trp Cys Val Val Met Leu
                85                  90                  95      
Ile Leu Ser Asn Ala Arg Leu Phe Leu Glu Asn Leu Ile Lys Tyr Gly
            100                 105                 110         
Ile Leu Val Asp Pro Ile Gln Val Val Ser Leu Phe Leu Lys Asp Pro
        115                 120                 125             
Tyr Ser Trp Pro Ala Pro Cys Leu Val Ile Ala Ala Asn Val Phe Ala
    130                 135                 140                 
Val Ala Ala Phe Gln Val Glu Lys Arg Leu Ala Val Gly Ala Leu Thr
145                 150                 155                 160 
Glu Gln Ala Gly Leu Leu Leu His Val Ala Asn Leu Ala Thr Ile Leu
                165                 170                 175     
Cys Phe Pro Ala Ala Val Val Leu Leu Val Glu Ser Ile Thr Pro Val
            180                 185                 190         
Gly Ser Leu Leu Ala Leu Met Ala His Thr Ile Leu Phe Leu Lys Leu
        195                 200                 205             
Phe Ser Tyr Arg Asp Val Asn Ser Trp Cys Arg Arg Ala Arg Ala Lys
    210                 215                 220                 
Ala Ala Ser Ala Gly Lys Lys Ala Ser Ser Ala Ala Ala Pro His Thr
225                 230                 235                 240 
Val Ser Tyr Pro Asp Asn Leu Thr Tyr Arg Asp Leu Tyr Tyr Phe Leu
                245                 250                 255     
Phe Ala Pro Thr Leu Cys Tyr Glu Leu Asn Phe Pro Arg Ser Pro Arg
            260                 265                 270         
Ile Arg Lys Arg Phe Leu Leu Arg Arg Ile Leu Glu Met Leu Phe Phe
        275                 280                 285             
Thr Gln Leu Gln Val Gly Leu Ile Gln Gln Trp Met Val Pro Thr Ile
    290                 295                 300                 
Gln Asn Ser Met Lys Pro Phe Lys Asp Met Asp Tyr Ser Arg Ile Ile
305                 310                 315                 320 
Glu Arg Leu Leu Lys Leu Ala Val Pro Asn His Leu Ile Trp Leu Ile
                325                 330                 335     
Phe Phe Tyr Trp Leu Phe His Ser Cys Leu Asn Ala Val Ala Glu Leu
            340                 345                 350         
Met Gln Phe Gly Asp Arg Glu Phe Tyr Arg Asp Trp Trp Asn Ser Glu
        355                 360                 365             
Ser Val Thr Tyr Phe Trp Gln Asn Trp Asn Ile Pro Val His Lys Trp
    370                 375                 380                 
Cys Ile Arg His Phe Tyr Lys Pro Met Leu Arg Arg Gly Ser Ser Lys
385                 390                 395                 400 
Trp Met Ala Arg Thr Gly Val Phe Leu Ala Ser Ala Phe Phe His Glu
                405                 410                 415     
Tyr Leu Val Ser Val Pro Leu Arg Met Phe Arg Leu Trp Ala Phe Thr
            420                 425                 430         
Gly Met Met Ala Gln Ile Pro Leu Ala Trp Phe Val Gly Arg Phe Phe
        435                 440                 445             
Gln Gly Asn Tyr Gly Asn Ala Ala Val Trp Leu Ser Leu Ile Ile Gly
    450                 455                 460                 
Gln Pro Ile Ala Val Leu Met Tyr Val His Asp Tyr Tyr Val Leu Asn
465                 470                 475                 480 
Tyr Glu Ala Pro Ala Ala Glu Ala
                485             


<210> 2
<211> 388
<212> PRT
<213> Homo sapiens

<400> 2
Met Lys Thr Leu Ile Ala Ala Tyr Ser Gly Val Leu Arg Gly Glu Arg
 1               5                  10                  15      
Gln Ala Glu Ala Asp Arg Ser Gln Arg Ser His Gly Gly Pro Ala Leu
            20                  25                  30          
Ser Arg Glu Gly Ser Gly Arg Trp Gly Thr Gly Ser Ser Ile Leu Ser
        35                  40                  45              
Ala Leu Gln Asp Leu Phe Ser Val Thr Trp Leu Asn Arg Ser Lys Val
    50                  55                  60                  
Glu Lys Gln Leu Gln Val Ile Ser Val Leu Gln Trp Val Leu Ser Phe
65                  70                  75                  80  
Leu Val Leu Gly Val Ala Cys Ser Ala Ile Leu Met Tyr Ile Phe Cys
                85                  90                  95      
Thr Asp Cys Trp Leu Ile Ala Val Leu Tyr Phe Thr Trp Leu Val Phe
            100                 105                 110         
Asp Trp Asn Thr Pro Lys Lys Gly Gly Arg Arg Ser Gln Trp Val Arg
        115                 120                 125             
Asn Trp Ala Val Trp Arg Tyr Phe Arg Asp Tyr Phe Pro Ile Gln Leu
    130                 135                 140                 
Val Lys Thr His Asn Leu Leu Thr Thr Arg Asn Tyr Ile Phe Gly Tyr
145                 150                 155                 160 
His Pro His Gly Ile Met Gly Leu Gly Ala Phe Cys Asn Phe Ser Thr
                165                 170                 175     
Glu Ala Thr Glu Val Ser Lys Lys Phe Pro Gly Ile Arg Pro Tyr Leu
            180                 185                 190         
Ala Thr Leu Ala Gly Asn Phe Arg Met Pro Val Leu Arg Glu Tyr Leu
        195                 200                 205             
Met Ser Gly Gly Ile Cys Pro Val Ser Arg Asp Thr Ile Asp Tyr Leu
    210                 215                 220                 
Leu Ser Lys Asn Gly Ser Gly Asn Ala Ile Ile Ile Val Val Gly Gly
225                 230                 235                 240 
Ala Ala Glu Ser Leu Ser Ser Met Pro Gly Lys Asn Ala Val Thr Leu
                245                 250                 255     
Arg Asn Arg Lys Gly Phe Val Lys Leu Ala Leu Arg His Gly Ala Asp
            260                 265                 270         
Leu Val Pro Ile Tyr Ser Phe Gly Glu Asn Glu Val Tyr Lys Gln Val
        275                 280                 285             
Ile Phe Glu Glu Gly Ser Trp Gly Arg Trp Val Gln Lys Lys Phe Gln
    290                 295                 300                 
Lys Tyr Ile Gly Phe Ala Pro Cys Ile Phe His Gly Arg Gly Leu Phe
305                 310                 315                 320 
Ser Ser Asp Thr Trp Gly Leu Val Pro Tyr Ser Lys Pro Ile Thr Thr
                325                 330                 335     
Val Val Gly Glu Pro Ile Thr Ile Pro Lys Leu Glu His Pro Thr Gln
            340                 345                 350         
Gln Asp Ile Asp Leu Tyr His Thr Met Tyr Met Glu Ala Leu Val Lys
        355                 360                 365             
Leu Phe Asp Lys His Lys Thr Lys Phe Gly Leu Pro Glu Thr Glu Val
    370                 375                 380                 
Leu Glu Val Asn
385             


<210> 3
<211> 550
<212> PRT
<213> Homo sapiens

<400> 3
Met Val Gly Glu Glu Lys Met Ser Leu Arg Asn Arg Leu Ser Lys Ser
 1               5                  10                  15      
Arg Glu Asn Pro Glu Glu Asp Glu Asp Gln Arg Asn Pro Ala Lys Glu
            20                  25                  30          
Ser Leu Glu Thr Pro Ser Asn Gly Arg Ile Asp Ile Lys Gln Leu Ile
        35                  40                  45              
Ala Lys Lys Ile Lys Leu Thr Ala Glu Ala Glu Glu Leu Lys Pro Phe
    50                  55                  60                  
Phe Met Lys Glu Val Gly Ser His Phe Asp Asp Phe Val Thr Asn Leu
65                  70                  75                  80  
Ile Glu Lys Ser Ala Ser Leu Asp Asn Gly Gly Cys Ala Leu Thr Thr
                85                  90                  95      
Phe Ser Val Leu Glu Gly Glu Lys Asn Asn His Arg Ala Lys Asp Leu
            100                 105                 110         
Arg Ala Pro Pro Glu Gln Gly Lys Ile Phe Ile Ala Arg Arg Ser Leu
        115                 120                 125             
Leu Asp Glu Leu Leu Glu Val Asp His Ile Arg Thr Ile Tyr His Met
    130                 135                 140                 
Phe Ile Ala Leu Leu Ile Leu Phe Ile Leu Ser Thr Leu Val Val Asp
145                 150                 155                 160 
Tyr Ile Asp Glu Gly Arg Leu Val Leu Glu Phe Ser Leu Leu Ser Tyr
                165                 170                 175     
Ala Phe Gly Lys Phe Pro Thr Val Val Trp Thr Trp Trp Ile Met Phe
            180                 185                 190         
Leu Ser Thr Phe Ser Val Pro Tyr Phe Leu Phe Gln His Trp Ala Thr
        195                 200                 205             
Gly Tyr Ser Lys Ser Ser His Pro Leu Ile Arg Ser Leu Phe His Gly
    210                 215                 220                 
Phe Leu Phe Met Ile Phe Gln Ile Gly Val Leu Gly Phe Gly Pro Thr
225                 230                 235                 240 
Tyr Val Val Leu Ala Tyr Thr Leu Pro Pro Ala Ser Arg Phe Ile Ile
                245                 250                 255     
Ile Phe Glu Gln Ile Arg Phe Val Met Lys Ala His Ser Phe Val Arg
            260                 265                 270         
Glu Asn Val Pro Arg Val Leu Asn Ser Ala Lys Glu Lys Ser Ser Thr
        275                 280                 285             
Val Pro Ile Pro Thr Val Asn Gln Tyr Leu Tyr Phe Leu Phe Ala Pro
    290                 295                 300                 
Thr Leu Ile Tyr Arg Asp Ser Tyr Pro Arg Asn Pro Thr Val Arg Trp
305                 310                 315                 320 
Gly Tyr Val Ala Met Lys Phe Ala Gln Val Phe Gly Cys Phe Phe Tyr
                325                 330                 335     
Val Tyr Tyr Ile Phe Glu Arg Leu Cys Ala Pro Leu Phe Arg Asn Ile
            340                 345                 350         
Lys Gln Glu Pro Phe Ser Ala Arg Val Leu Val Leu Cys Val Phe Asn
        355                 360                 365             
Ser Ile Leu Pro Gly Val Leu Ile Leu Phe Leu Thr Phe Phe Ala Phe
    370                 375                 380                 
Leu His Cys Trp Leu Asn Ala Phe Ala Glu Met Leu Arg Phe Gly Asp
385                 390                 395                 400 
Arg Met Phe Tyr Lys Asp Trp Trp Asn Ser Thr Ser Tyr Ser Asn Tyr
                405                 410                 415     
Tyr Arg Thr Trp Asn Val Val Val His Asp Trp Leu Tyr Tyr Tyr Ala
            420                 425                 430         
Tyr Lys Asp Phe Leu Trp Phe Phe Ser Lys Arg Phe Lys Ser Ala Ala
        435                 440                 445             
Met Leu Ala Val Phe Ala Val Ser Ala Val Val His Glu Tyr Ala Leu
    450                 455                 460                 
Ala Val Cys Leu Ser Phe Phe Tyr Pro Val Leu Phe Val Leu Phe Met
465                 470                 475                 480 
Phe Phe Gly Met Ala Phe Asn Phe Ile Val Asn Asp Ser Arg Lys Lys
                485                 490                 495     
Pro Ile Trp Asn Val Leu Met Trp Thr Ser Leu Phe Leu Gly Asn Gly
            500                 505                 510         
Val Leu Leu Cys Phe Tyr Ser Gln Glu Trp Tyr Ala Arg Gln His Cys
        515                 520                 525             
Pro Leu Lys Asn Pro Thr Phe Leu Asp Tyr Val Arg Pro Arg Ser Trp
    530                 535                 540                 
Thr Cys Arg Tyr Val Phe
545                 550 


<210> 4
<211> 522
<212> PRT
<213> Homo sapiens

<400> 4
Met Glu Pro Gly Gly Ala Arg Leu Arg Leu Gln Arg Thr Glu Gly Leu
 1               5                  10                  15      
Gly Gly Glu Arg Glu Arg Gln Pro Cys Gly Asp Gly Asn Thr Glu Thr
            20                  25                  30          
His Arg Ala Pro Asp Leu Val Gln Trp Thr Arg His Met Glu Ala Val
        35                  40                  45              
Lys Ala Gln Leu Leu Glu Gln Ala Gln Gly Gln Leu Arg Glu Leu Leu
    50                  55                  60                  
Asp Arg Ala Met Arg Glu Ala Ile Gln Ser Tyr Pro Ser Gln Asp Lys
65                  70                  75                  80  
Pro Leu Pro Pro Pro Pro Pro Gly Ser Leu Ser Arg Thr Gln Glu Pro
                85                  90                  95      
Ser Leu Gly Lys Gln Lys Val Phe Ile Ile Arg Lys Ser Leu Leu Asp
            100                 105                 110         
Glu Leu Met Glu Val Gln His Phe Arg Thr Ile Tyr His Met Phe Ile
        115                 120                 125             
Ala Gly Leu Cys Val Phe Ile Ile Ser Thr Leu Ala Ile Asp Phe Ile
    130                 135                 140                 
Asp Glu Gly Arg Leu Leu Leu Glu Phe Asp Leu Leu Ile Phe Ser Phe
145                 150                 155                 160 
Gly Gln Leu Pro Leu Ala Leu Val Thr Trp Val Pro Met Phe Leu Ser
                165                 170                 175     
Thr Leu Leu Ala Pro Tyr Gln Ala Leu Arg Leu Trp Ala Arg Gly Thr
            180                 185                 190         
Trp Thr Gln Ala Thr Gly Leu Gly Cys Ala Leu Leu Ala Ala His Ala
        195                 200                 205             
Val Val Leu Cys Ala Leu Pro Val His Val Ala Val Glu His Gln Leu
    210                 215                 220                 
Pro Pro Ala Ser Arg Cys Val Leu Val Phe Glu Gln Val Arg Phe Leu
225                 230                 235                 240 
Met Lys Ser Tyr Ser Phe Leu Arg Glu Ala Val Pro Gly Thr Leu Arg
                245                 250                 255     
Ala Arg Arg Gly Glu Gly Ile Gln Ala Pro Ser Phe Ser Ser Tyr Leu
            260                 265                 270         
Tyr Phe Leu Phe Cys Pro Thr Leu Ile Tyr Arg Glu Thr Tyr Pro Arg
        275                 280                 285             
Thr Pro Tyr Val Arg Trp Asn Tyr Val Ala Lys Asn Phe Ala Gln Ala
    290                 295                 300                 
Leu Gly Cys Val Leu Tyr Ala Cys Phe Ile Leu Gly Arg Leu Cys Val
305                 310                 315                 320 
Pro Val Phe Ala Asn Met Ser Arg Glu Pro Phe Ser Thr Arg Ala Leu
                325                 330                 335     
Val Leu Ser Ile Leu His Ala Thr Leu Pro Gly Ile Phe Met Leu Leu
            340                 345                 350         
Leu Ile Phe Phe Ala Phe Leu His Cys Trp Leu Asn Ala Phe Ala Glu
        355                 360                 365             
Met Leu Arg Phe Gly Asp Arg Met Phe Tyr Arg Asp Trp Trp Asn Ser
    370                 375                 380                 
Thr Ser Phe Ser Asn Tyr Tyr Arg Thr Trp Asn Val Val Val His Asp
385                 390                 395                 400 
Trp Leu Tyr Ser Tyr Val Tyr Gln Asp Gly Leu Arg Leu Leu Gly Ala
                405                 410                 415     
Arg Ala Arg Gly Val Ala Met Leu Gly Val Phe Leu Val Ser Ala Val
            420                 425                 430         
Ala His Glu Tyr Ile Phe Cys Phe Val Leu Gly Phe Phe Tyr Pro Val
        435                 440                 445             
Met Leu Ile Leu Phe Leu Val Ile Gly Gly Met Leu Asn Phe Met Met
    450                 455                 460                 
His Asp Gln Arg Thr Gly Pro Ala Trp Asn Val Leu Met Trp Thr Met
465                 470                 475                 480 
Leu Phe Leu Gly Gln Gly Ile Gln Val Ser Leu Tyr Cys Gln Glu Trp
                485                 490                 495     
Tyr Ala Arg Arg His Cys Pro Leu Pro Gln Ala Thr Phe Trp Gly Leu
            500                 505                 510         
Val Thr Pro Arg Ser Trp Ser Cys His Thr
        515                 520         


<210> 5
<211> 191
<212> PRT
<213> Hepatitis C Virus

<400> 5
Met Ser Thr Asn Pro Lys Pro Gln Arg Lys Thr Lys Arg Ser Thr Asn
 1               5                  10                  15      
Arg Arg Pro Gln Asp Val Lys Phe Pro Gly Gly Gly Gln Ile Val Gly
            20                  25                  30          
Gly Val Tyr Leu Leu Pro Arg Arg Gly Pro Arg Leu Gly Val Arg Ala
        35                  40                  45              
Thr Arg Lys Thr Ser Glu Arg Ser Gln Pro Arg Gly Arg Arg Gln Pro
    50                  55                  60                  
Ile Pro Lys Ala Arg Gln Pro Glu Gly Arg Ala Trp Ala Gln Pro Gly
65                  70                  75                  80  
Tyr Pro Trp Pro Leu Tyr Gly Asn Glu Gly Met Gly Trp Ala Gly Trp
                85                  90                  95      
Leu Leu Ser Pro Arg Gly Ser Arg Pro Ser Trp Gly Pro Thr Asp Pro
            100                 105                 110         
Arg Arg Arg Ser Arg Asn Leu Gly Lys Val Ile Asp Thr Leu Thr Cys
        115                 120                 125             
Gly Phe Ala Asp Leu Met Gly Tyr Ile Pro Leu Val Gly Ala Pro Leu
    130                 135                 140                 
Gly Gly Ala Ala Arg Ala Leu Ala His Gly Val Arg Val Leu Glu Asp
145                 150                 155                 160 
Gly Val Asn Tyr Ala Thr Gly Asn Leu Pro Gly Cys Ser Phe Ser Ile
                165                 170                 175     
Phe Leu Leu Ala Leu Leu Ser Cys Leu Thr Ile Pro Ala Ser Ala
            180                 185                 190     


<210> 6
<211> 1467
<212> DNA
<213> Homo sapiens

<400> 6
atgggcgacc gcggcagctc ccggcgccgg aggacagggt cgcggccctc gagccacggc 60
ggcggcgggc ctgcggcggc ggaagaggag gtgcgggacg ccgctgcggg ccccgacgtg 120
ggagccgcgg gggacgcgcc agccccggcc cccaacaagg acggagacgc cggcgtgggc 180
agcggccact gggagctgag gtgccatcgc ctgcaggatt ctttattcag ctctgacagt 240
ggcttcagca actaccgtgg catcctgaac tggtgtgtgg tgatgctgat cttgagcaat 300
gcccggttat ttctggagaa cctcatcaag tatggcatcc tggtggaccc catccaggtg 360
gtttctctgt tcctgaagga tccctatagc tggcccgccc catgcctggt tattgcggcc 420
aatgtctttg ctgtggctgc attccaggtt gagaagcgcc tggcggtggg tgccctgacg 480
gagcaggcgg gactgctgct gcacgtggcc aacctggcca ccattctgtg tttcccagcg 540
gctgtggtct tactggttga gtctatcact ccagtgggct ccctgctggc gctgatggcg 600
cacaccatcc tcttcctcaa gctcttctcc taccgcgacg tcaactcatg gtgccgcagg 660
gccagggcca aggctgcctc tgcagggaag aaggccagca gtgctgctgc cccgcacacc 720
gtgagctacc cggacaatct gacctaccgc gatctctact acttcctctt cgcccccacc 780
ttgtgctacg agctcaactt tccccgctct ccccgcatcc ggaagcgctt tctgctgcga 840
cggatccttg agatgctgtt cttcacccag ctccaggtgg ggctgatcca gcagtggatg 900
gtccccacca tccagaactc catgaagccc ttcaaggaca tggactactc acgcatcatc 960
gagcgcctcc tgaagctggc ggtccccaat cacctcatct ggctcatctt cttctactgg 1020
ctcttccact cctgcctgaa tgccgtggct gagctcatgc agtttggaga ccgggagttc 1080
taccgggact ggtggaactc cgagtctgtc acctacttct ggcagaactg gaacatccct 1140
gtgcacaagt ggtgcatcag acacttctac aagcccatgc ttcgacgggg cagcagcaag 1200
tggatggcca ggacaggggt gttcctggcc tcggccttct tccacgagta cctggtgagc 1260
gtccctctgc gaatgttccg cctctgggcg ttcacgggca tgatggctca gatcccactg 1320
gcctggttcg tgggccgctt tttccagggc aactatggca acgcagctgt gtggctgtcg 1380
ctcatcatcg gacagccaat agccgtcctc atgtacgtcc acgactacta cgtgctcaac 1440
tatgaggccc cagcggcaga ggcctga                                     1467

<210> 7
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 7
gcccauggcc ucagcccgca                                             20

<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 8
acgccggcgu cuccguccuu                                             20

<210> 9
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 9
cugcaggcga uggcaccuca                                             20

<210> 10
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 10
cucccagcug gcaucagacu                                             20

<210> 11
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 11
cuugagcaau gcccgguua                                              19

<210> 12
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 12
caauagccgu ccucaugua                                              19

<210> 13
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 13
ucaaggacau ggacuacuc                                              19

<210> 14
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 14
gcuguggucu uacugguug                                              19

<210> 15
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 15
gaacacaccc aagaaaggu                                              19

<210> 16
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 16
ggaacatccc tgtgcacaa                                              19

<210> 17
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 17
gcatcagaca cttctacaa                                              19

<210> 18
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 18
gcgaaagcca cttctcata                                              19

<210> 19
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Oligonucleotide

<400> 19
cttacgctga gtacttcga                                              19
