                         SEQUENCE LISTING

<110>  Parco Tecnologico Padano
 
<120>  PRODUCTION OF NGF IN PLANT

<130>  Bw535R/RVP

<160>  14    

<170>  PatentIn version 3.3

<210>  1
<211>  726
<212>  DNA
<213>  Homo sapiens


<220>  human pre-pro-NGF
<221>  CDS
<222>  (1)..(726)

<400>  1
atg tcc atg ttg ttc tac act ctg atc aca gct ttt ctg atc ggc ata       48
          

cag gcg gaa cca cac tca gag agc aat gtc cct gca gga cac acc atc       96
            

ccc caa gtc cac tgg act aaa ctt cag cat tcc ctt gac act gcc ctt      144
                 

cgc aga gcc cgc agc gcc ccg gca gcg gcg ata gct gca cgc gtg gcg      192
                      

ggg cag acc cgc aac att act gtg gac ccc agg ctg ttt aaa aag cgg      240
        

cga ctc cgt tca ccc cgt gtg ctg ttt agc acc cag cct ccc cgt gaa      288
          

gct gca gac act cag gat ctg gac ttc gag gtc ggt ggt gct gcc ccc      336
             

ttc aac agg act cac agg agc aag cgg tca tca tcc cat ccc atc ttc      384
               

cac agg ggc gaa ttc tcg gtg tgt gac agt gtc agc gtg tgg gtt ggg      432
                

gat aag acc acc gcc aca gac atc aag ggc aag gag gtg atg gtg ttg      480
        

gga gag gtg aac att aac aac agt gta ttc aaa cag tac ttt ttt gag      528
            

acc aag tgc cgg gac cca aat ccc gtt gac agc ggg tgc cgg ggc att      576
              

gac tca aag cac tgg aac tca tat tgt acc acg act cac acc ttt gtc      624
                 

aag gcg ctg acc atg gat ggc aag cag gct gcc tgg cgg ttt atc cgg      672
                      

ata gat acg gcc tgt gtg tgt gtg ctc agc agg aag gct gtg aga aga      720
           

gcc tga                                                              726
                                                                    
                                                                        


<210>  2
<211>  241
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ser Met Leu Phe Tyr Thr Leu Ile Thr Ala Phe Leu Ile Gly Ile 
1               5                   10                  15      


Gln Ala Glu Pro His Ser Glu Ser Asn Val Pro Ala Gly His Thr Ile 
            20                  25                  30          


Pro Gln Val His Trp Thr Lys Leu Gln His Ser Leu Asp Thr Ala Leu 
        35                  40                  45              


Arg Arg Ala Arg Ser Ala Pro Ala Ala Ala Ile Ala Ala Arg Val Ala 
    50                  55                  60                  


Gly Gln Thr Arg Asn Ile Thr Val Asp Pro Arg Leu Phe Lys Lys Arg 
65                  70                  75                  80  


Arg Leu Arg Ser Pro Arg Val Leu Phe Ser Thr Gln Pro Pro Arg Glu 
                85                  90                  95      


Ala Ala Asp Thr Gln Asp Leu Asp Phe Glu Val Gly Gly Ala Ala Pro 
            100                 105                 110         


Phe Asn Arg Thr His Arg Ser Lys Arg Ser Ser Ser His Pro Ile Phe 
        115                 120                 125             


His Arg Gly Glu Phe Ser Val Cys Asp Ser Val Ser Val Trp Val Gly 
    130                 135                 140                 


Asp Lys Thr Thr Ala Thr Asp Ile Lys Gly Lys Glu Val Met Val Leu 
145                 150                 155                 160 


Gly Glu Val Asn Ile Asn Asn Ser Val Phe Lys Gln Tyr Phe Phe Glu 
                165                 170                 175     


Thr Lys Cys Arg Asp Pro Asn Pro Val Asp Ser Gly Cys Arg Gly Ile 
            180                 185                 190         


Asp Ser Lys His Trp Asn Ser Tyr Cys Thr Thr Thr His Thr Phe Val 
        195                 200                 205             


Lys Ala Leu Thr Met Asp Gly Lys Gln Ala Ala Trp Arg Phe Ile Arg 
    210                 215                 220                 


Ile Asp Thr Ala Cys Val Cys Val Leu Ser Arg Lys Ala Val Arg Arg 
225                 230                 235                 240 


Ala 
    


<210>  3
<211>  18
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ser Met Leu Phe Tyr Thr Leu Ile Thr Ala Phe Leu Ile Gly Ile 
1               5                   10                  15      


Gln Ala 
        


<210>  4
<211>  103
<212>  PRT
<213>  Homo sapiens

<400>  4

Glu Pro His Ser Glu Ser Asn Val Pro Ala Gly His Thr Ile Pro Gln 
1               5                   10                  15      


Ala His Trp Thr Lys Leu Gln His Ser Leu Asp Thr Ala Leu Arg Arg 
            20                  25                  30          


Ala Arg Ser Ala Pro Ala Ala Ala Ile Ala Ala Arg Val Ala Gly Gln 
        35                  40                  45              


Thr Arg Asn Ile Thr Val Asp Pro Arg Leu Phe Lys Lys Arg Arg Leu 
    50                  55                  60                  


Arg Ser Pro Arg Val Leu Phe Ser Thr Gln Pro Pro Arg Glu Ala Ala 
65                  70                  75                  80  


Asp Thr Gln Asp Leu Asp Phe Glu Val Gly Gly Ala Ala Pro Phe Asn 
                85                  90                  95      


Arg Thr His Arg Ser Lys Arg 
            100             


<210>  5
<211>  2095
<212>  DNA
<213>  Artificial

<220>  expression cassette
<223>  expression cassette for the expression in plant of
       rh pre-pro-NGF

<400>  5
aagcttgcat gcctgcaggt ctcagaagac cagagggcta ttgagacttt tcaacaaagg     60

gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg    120

acagtagaaa aggaagatgg cttctacaaa tgccatcatt gcgataaagg aaaggctatc    180

gttcaagaat gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat    240

cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgataacat    300

ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca    360

gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg    420

cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct tctacaaatg    480

ccatcattgc gataaaggaa aggctatcgt tcaagaatgc ctctaccgac agtggtccca    540

aagatggacc cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt    600

caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact    660

atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga    720

gaattcacaa cacaaatcag atttatagag agatttataa aaaaaaaaaa acatatggag    780

tggagctgga tctttctctt tctcctctca ggaactgcag gtgttcactc catgaccatg    840

ttgttctaca ctctgatcac agcttttctg atcggcatac aggcggaacc acactcagag    900

agcaatgtcc ctgcaggaca caccatcccc caagtccact ggactaaact tcagcattcc    960

cttgacactg cccttcgcag agcccgcagc gccccggcag cggcgatagc tgcacgcgtg   1020

gcggggcaga cccgcaacat tactgtggac cccaggctgt ttaaaaagcg gcgactccgt   1080

tcaccccgtg tgctgtttag cacccagcct ccccgtgaag ctgcagacac tcaggatctg   1140

gacttcgagg tcggtggtgc tgcccccttc aacaggactc acaggagcaa gcggtcatca   1200

tcccatccca tcttccacag gggcgaattc tcggtgtgtg acagtgtcag cgtgtgggtt   1260

ggggataaga ccaccgccac agacatcaag ggcaaggagg tgatggtgtt gggagaggtg   1320

aacattaaca acagtgtatt caaacagtac ttttttgaga ccaagtgccg ggacccaaat   1380

cccgttgaca gcgggtgccg gggcattgac tcaaagcact ggaactcata ttgtaccacg   1440

actcacacct ttgtcaaggc gctgaccatg gatggcaagc aggctgcctg gcggtttatc   1500

cggatagata cggcctgtgt gtgtgtgctc agcaggaagg ctgtgagaag agccgtcgac   1560

gaaaatcttt attttcaagg agtcgacaag gatgaacttc atcaccatca tcaccattaa   1620

ctcgaggggt agtcaagatg cataataaat aacggattgt gtccgtaatc acacgtggtg   1680

cgtacgataa cgcatagtgt ttttccctcc acttaaatcg aagggttgtg tcttggatcg   1740

cgcgggtcaa atgtatatgg ttcatataca tccgcaggca cgtaataaag cgaggggttc   1800

gaatcccccc gttacccccg gtaggggccc aggtaccggc gcgcctctag agtccgcaaa   1860

aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga   1920

gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat   1980

aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt   2040

cctaaaacca aaatccagtg acctgcaggc atgcaagctc ctagctagca tcgac        2095


<210>  6
<211>  1920
<212>  DNA
<213>  Artificial

<220>  expression cassette
<223>  expression cassette for the expression in plant of
       hr pre-pro-NGF

<400>  6
aagcttgcat gcctgcaggt ctcagaagac cagagggcta ttgagacttt tcaacaaagg     60

gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg    120

acagtagaaa aggaagatgg cttctacaaa tgccatcatt gcgataaagg aaaggctatc    180

gttcaagaat gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat    240

cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgataacat    300

ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca    360

gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg    420

cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct tctacaaatg    480

ccatcattgc gataaaggaa aggctatcgt tcaagaatgc ctctaccgac agtggtccca    540

aagatggacc cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt    600

caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact    660

atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga    720

gaattcacaa cacaaatcag atttatagag agatttataa aaaaaaaaaa acatatggag    780

tggagctgga tctttctctt tctcctctca ggaactgcag gtgttcactc catgaccatg    840

ttgttctaca ctctgatcac agcttttctg atcggcatac aggcggaacc acactcagag    900

agcaatgtcc ctgcaggaca caccatcccc caagtccact ggactaaact tcagcattcc    960

cttgacactg cccttcgcag agcccgcagc gccccggcag cggcgatagc tgcacgcgtg   1020

gcggggcaga cccgcaacat tactgtggac cccaggctgt ttaaaaagcg gcgactccgt   1080

tcaccccgtg tgctgtttag cacccagcct ccccgtgaag ctgcagacac tcaggatctg   1140

gacttcgagg tcggtggtgc tgcccccttc aacaggactc acaggagcaa gcggtcatca   1200

tcccatccca tcttccacag gggcgaattc tcggtgtgtg acagtgtcag cgtgtgggtt   1260

ggggataaga ccaccgccac agacatcaag ggcaaggagg tgatggtgtt gggagaggtg   1320

aacattaaca acagtgtatt caaacagtac ttttttgaga ccaagtgccg ggacccaaat   1380

cccgttgaca gcgggtgccg gggcattgac tcaaagcact ggaactcata ttgtaccacg   1440

actcacacct ttgtcaaggc gctgaccatg gatggcaagc aggctgcctg gcggtttatc   1500

cggatagata cggcctgtgt gtgtgtgctc agcaggaagg ctgtgagaag agccgtcgac   1560

ggaggtggag gttctgcggc cgctcgtgga tctgagaaag atgagctcta aactcgaggg   1620

gtagtcaaga tgcataataa ataacggatt gtgtccgtaa tcacacgtgg tgcgtacgat   1680

aacgcatagt gtttttccct ccacttaaat cgaagggttg tgtcttggat cgcgcgggtc   1740

aaatgtatat ggttcatata catccgcagg cacgtaataa agcgaggggt tcgaatcccc   1800

ccgttacccc cggtaggggc ccaggtaccg gcgcgcctct agagtccgca aaaatcacca   1860

gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc   1920


<210>  7
<211>  2068
<212>  DNA
<213>  Artificial

<220>  expression cassette
<223>  expression cassette for the expression in plant of 
       hr pre-pro-NGF

<400>  7
aagcttgcat gcctgcaggt ctcagaagac cagagggcta ttgagacttt tcaacaaagg     60

gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg    120

acagtagaaa aggaagatgg cttctacaaa tgccatcatt gcgataaagg aaaggctatc    180

gttcaagaat gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat    240

cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgataacat    300

ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca    360

gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg    420

cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct tctacaaatg    480

ccatcattgc gataaaggaa aggctatcgt tcaagaatgc ctctaccgac agtggtccca    540

aagatggacc cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt    600

caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact    660

atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga    720

gaattcacaa cacaaatcag atttatagag agatttataa aaaaaaaaaa acatatggag    780

tggagctgga tctttctctt tctcctctca ggaactgcag gtgttcactc catgaccatg    840

ttgttctaca ctctgatcac agcttttctg atcggcatac aggcggaacc acactcagag    900

agcaatgtcc ctgcaggaca caccatcccc caagtccact ggactaaact tcagcattcc    960

cttgacactg cccttcgcag agcccgcagc gccccggcag cggcgatagc tgcacgcgtg   1020

gcggggcaga cccgcaacat tactgtggac cccaggctgt ttaaaaagcg gcgactccgt   1080

tcaccccgtg tgctgtttag cacccagcct ccccgtgaag ctgcagacac tcaggatctg   1140

gacttcgagg tcggtggtgc tgcccccttc aacaggactc acaggagcaa gcggtcatca   1200

tcccatccca tcttccacag gggcgaattc tcggtgtgtg acagtgtcag cgtgtgggtt   1260

ggggataaga ccaccgccac agacatcaag ggcaaggagg tgatggtgtt gggagaggtg   1320

aacattaaca acagtgtatt caaacagtac ttttttgaga ccaagtgccg ggacccaaat   1380

cccgttgaca gcgggtgccg gggcattgac tcaaagcact ggaactcata ttgtaccacg   1440

actcacacct ttgtcaaggc gctgaccatg gatggcaagc aggctgcctg gcggtttatc   1500

cggatagata cggcctgtgt gtgtgtgctc agcaggaagg ctgtgagaag agccgtcgac   1560

aaggatgaac ttcatcacca tcatcaccat taactcgagg ggtagtcaag atgcataata   1620

aataacggat tgtgtccgta atcacacgtg gtgcgtacga taacgcatag tgtttttccc   1680

tccacttaaa tcgaagggtt gtgtcttgga tcgcgcgggt caaatgtata tggttcatat   1740

acatccgcag gcacgtaata aagcgagggg ttcgaatccc cccgttaccc ccggtagggg   1800

cccaggtacc ggcgcgcctc tagagtccgc aaaaatcacc agtctctctc tacaaatcta   1860

tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt   1920

tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt   1980

tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacctgca   2040

ggcatgcaag ctcctagcta gcatcgac                                      2068


<210>  8
<211>  76
<212>  DNA
<213>  Artificial

<220>  misc_feature
<223>  oligo for the insertion of TEV-KDEL-6XHis-Tag

<400>  8
tcgacgaaaa tctttatttt caaggagtcg acaaggatga acttcatcac catcatcacc     60

attaactcga ggagct                                                     76


<210>  9
<211>  23
<212>  DNA
<213>  Artificial

<220>  misc_feature
<223>  pNGF sense primer 

<400>  9
cccaataaca gttttaccaa ggg                                             23


<210>  10
<211>  21
<212>  DNA
<213>  Artificial

<220>  misc_feature
<223>  pNGF antisense primer 

<400>  10
caggtcaggc tcttctcaca g                                               21


<210>  11
<211>  28
<212>  DNA
<213>  Artificial

<220>  misc_feature
<223>  pNGF sense Bsph primer 

<400>  11
cctcatgacc atgttgttct acactctg                                        28


<210>  12
<211>  29
<212>  DNA
<213>  Artificial

<220>  misc_feature
<223>  pNGF antisense Sal primer 

<400>  12
atagtcgacg gctcttctca cagccttcc                                       29


<210>  13
<211>  21
<212>  DNA
<213>  Artificial

<220>  misc_feature
<223>  p35S sense

<400>  13
cactgacgta agggatgacg c                                               21


<210>  14
<211>  7
<212>  PRT
<213>  Artificial

<220>  SITE
<223>  KDEL recognition site

<400>  14

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


