                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Hafnia phytase

<130>  11422.204-WO

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  1338
<212>  DNA
<213>  Hafnia alvei


<220>
<221>  CDS
<222>  (1)..(1338)

<220>
<221>  sig_peptide
<222>  (1)..(99)

<220>
<221>  mat_peptide
<222>  (100)..(1338)

<400>  1
atg aca atc tct ctg ttt aac cgt aat aaa ccc gct att gca cag cgt         48
Met Thr Ile Ser Leu Phe Asn Arg Asn Lys Pro Ala Ile Ala Gln Arg           
            -30                 -25                 -20                   

att tta tgt cct ctg atc gtg gct tta ttc tca ggt tta ccg gca tac         96
Ile Leu Cys Pro Leu Ile Val Ala Leu Phe Ser Gly Leu Pro Ala Tyr           
        -15                 -10                 -5                        

gcc agt gat acc gcc cct gct ggg ttc cag ttg gaa aag gtt gtt atc        144
Ala Ser Asp Thr Ala Pro Ala Gly Phe Gln Leu Glu Lys Val Val Ile           
-1  1               5                   10                  15            

cta agc aga cat ggt gta cgc gcg cca acc aaa atg aca caa acg atg        192
Leu Ser Arg His Gly Val Arg Ala Pro Thr Lys Met Thr Gln Thr Met           
                20                  25                  30                

cgc gac gtc aca cct cac cag tgg cct gaa tgg ccg gta aaa ctc ggc        240
Arg Asp Val Thr Pro His Gln Trp Pro Glu Trp Pro Val Lys Leu Gly           
            35                  40                  45                    

tat atc acg cca cgc ggc gaa cat ctg att agc ctg atg ggc ggt ttt        288
Tyr Ile Thr Pro Arg Gly Glu His Leu Ile Ser Leu Met Gly Gly Phe           
        50                  55                  60                        

tat cga gag cgc ttt cag caa caa ggt tta tta cct aag gat aac tgt        336
Tyr Arg Glu Arg Phe Gln Gln Gln Gly Leu Leu Pro Lys Asp Asn Cys           
    65                  70                  75                            

cct aca cca gat gcc gtg tat gtt tgg gca gac gtc gat caa cgc aca        384
Pro Thr Pro Asp Ala Val Tyr Val Trp Ala Asp Val Asp Gln Arg Thr           
80                  85                  90                  95            

cgt aaa acc ggc gag gct ttc tta gca ggt ctt gct ccc cag tgt gat        432
Arg Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Gln Cys Asp           
                100                 105                 110               

tta gcg atc cac cat cag caa aac act cag cag gcc gat ccg ctg ttc        480
Leu Ala Ile His His Gln Gln Asn Thr Gln Gln Ala Asp Pro Leu Phe           
            115                 120                 125                   

cac cct gtg aaa gcc ggt att tgt tcg atg gat aaa tca cag gta cac        528
His Pro Val Lys Ala Gly Ile Cys Ser Met Asp Lys Ser Gln Val His           
        130                 135                 140                       

gcc gcg gtt gaa aag cag gca ggc aca ccg att gag acg ctc aat caa        576
Ala Ala Val Glu Lys Gln Ala Gly Thr Pro Ile Glu Thr Leu Asn Gln           
    145                 150                 155                           

cgc tat caa gcc tct tta gcg ctg atg agt tcg gta ctc gat ttt cca        624
Arg Tyr Gln Ala Ser Leu Ala Leu Met Ser Ser Val Leu Asp Phe Pro           
160                 165                 170                 175           

aaa tcc ccc tat tgt cag cag cac aac att ggc aaa ctc tgc gat ttt        672
Lys Ser Pro Tyr Cys Gln Gln His Asn Ile Gly Lys Leu Cys Asp Phe           
                180                 185                 190               

tca cag gcg atg cct agc aga ctg gcg ata aat gac gac ggt aat aaa        720
Ser Gln Ala Met Pro Ser Arg Leu Ala Ile Asn Asp Asp Gly Asn Lys           
            195                 200                 205                   

gtg gct ctc gaa ggt gcc gtg gga ctt gca tcg acg ttg gct gaa att        768
Val Ala Leu Glu Gly Ala Val Gly Leu Ala Ser Thr Leu Ala Glu Ile           
        210                 215                 220                       

ttc ctg ctg gaa cac gct cag gga atg cct aaa gtg gct tgg ggg aat        816
Phe Leu Leu Glu His Ala Gln Gly Met Pro Lys Val Ala Trp Gly Asn           
    225                 230                 235                           

att cac act gag cag caa tgg aac tct ctg ttg aaa ttg cat aat gcg        864
Ile His Thr Glu Gln Gln Trp Asn Ser Leu Leu Lys Leu His Asn Ala           
240                 245                 250                 255           

cag ttt gac ttg atg tcg cgc acg ccc tat atc gcc aag cat aac ggt        912
Gln Phe Asp Leu Met Ser Arg Thr Pro Tyr Ile Ala Lys His Asn Gly           
                260                 265                 270               

act cca ctg ctg caa acc atc gcc cac gca ctg ggt tcc aat atc acg        960
Thr Pro Leu Leu Gln Thr Ile Ala His Ala Leu Gly Ser Asn Ile Thr           
            275                 280                 285                   

agt cgc cca ctg ccg gat att tcg cca gac aat aag atc ctg ttt att       1008
Ser Arg Pro Leu Pro Asp Ile Ser Pro Asp Asn Lys Ile Leu Phe Ile           
        290                 295                 300                       

gcc ggt cac gac acc aat att gcc aat att tct ggc atg tta ggg atg       1056
Ala Gly His Asp Thr Asn Ile Ala Asn Ile Ser Gly Met Leu Gly Met           
    305                 310                 315                           

aca tgg aca ctt ccg gga caa cca gat aac acg cct ccg ggt ggc gct       1104
Thr Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Ala           
320                 325                 330                 335           

ttg gtg ttt gaa cgc tgg gta gat aac gcg ggg aaa ccg tat gtt agc       1152
Leu Val Phe Glu Arg Trp Val Asp Asn Ala Gly Lys Pro Tyr Val Ser           
                340                 345                 350               

gtg aat atg gtg tat caa aca ctg gca cag ttg cac gac cag gcg ccg       1200
Val Asn Met Val Tyr Gln Thr Leu Ala Gln Leu His Asp Gln Ala Pro           
            355                 360                 365                   

cta acg ttg cag cat cct gcg ggc agc gta cga cta aac ata ccg ggt       1248
Leu Thr Leu Gln His Pro Ala Gly Ser Val Arg Leu Asn Ile Pro Gly           
        370                 375                 380                       

tgc agc gat caa acg ccc gat ggc tat tgc ccg ctc tcc acc ttc agc       1296
Cys Ser Asp Gln Thr Pro Asp Gly Tyr Cys Pro Leu Ser Thr Phe Ser           
    385                 390                 395                           

cgc tta gtc agc cac agc gtt gag cct gcg tgc cag ctt cct               1338
Arg Leu Val Ser His Ser Val Glu Pro Ala Cys Gln Leu Pro                   
400                 405                 410                               


<210>  2
<211>  446
<212>  PRT
<213>  Hafnia alvei

<400>  2

Met Thr Ile Ser Leu Phe Asn Arg Asn Lys Pro Ala Ile Ala Gln Arg 
            -30                 -25                 -20         


Ile Leu Cys Pro Leu Ile Val Ala Leu Phe Ser Gly Leu Pro Ala Tyr 
        -15                 -10                 -5              


Ala Ser Asp Thr Ala Pro Ala Gly Phe Gln Leu Glu Lys Val Val Ile 
-1  1               5                   10                  15  


Leu Ser Arg His Gly Val Arg Ala Pro Thr Lys Met Thr Gln Thr Met 
                20                  25                  30      


Arg Asp Val Thr Pro His Gln Trp Pro Glu Trp Pro Val Lys Leu Gly 
            35                  40                  45          


Tyr Ile Thr Pro Arg Gly Glu His Leu Ile Ser Leu Met Gly Gly Phe 
        50                  55                  60              


Tyr Arg Glu Arg Phe Gln Gln Gln Gly Leu Leu Pro Lys Asp Asn Cys 
    65                  70                  75                  


Pro Thr Pro Asp Ala Val Tyr Val Trp Ala Asp Val Asp Gln Arg Thr 
80                  85                  90                  95  


Arg Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Gln Cys Asp 
                100                 105                 110     


Leu Ala Ile His His Gln Gln Asn Thr Gln Gln Ala Asp Pro Leu Phe 
            115                 120                 125         


His Pro Val Lys Ala Gly Ile Cys Ser Met Asp Lys Ser Gln Val His 
        130                 135                 140             


Ala Ala Val Glu Lys Gln Ala Gly Thr Pro Ile Glu Thr Leu Asn Gln 
    145                 150                 155                 


Arg Tyr Gln Ala Ser Leu Ala Leu Met Ser Ser Val Leu Asp Phe Pro 
160                 165                 170                 175 


Lys Ser Pro Tyr Cys Gln Gln His Asn Ile Gly Lys Leu Cys Asp Phe 
                180                 185                 190     


Ser Gln Ala Met Pro Ser Arg Leu Ala Ile Asn Asp Asp Gly Asn Lys 
            195                 200                 205         


Val Ala Leu Glu Gly Ala Val Gly Leu Ala Ser Thr Leu Ala Glu Ile 
        210                 215                 220             


Phe Leu Leu Glu His Ala Gln Gly Met Pro Lys Val Ala Trp Gly Asn 
    225                 230                 235                 


Ile His Thr Glu Gln Gln Trp Asn Ser Leu Leu Lys Leu His Asn Ala 
240                 245                 250                 255 


Gln Phe Asp Leu Met Ser Arg Thr Pro Tyr Ile Ala Lys His Asn Gly 
                260                 265                 270     


Thr Pro Leu Leu Gln Thr Ile Ala His Ala Leu Gly Ser Asn Ile Thr 
            275                 280                 285         


Ser Arg Pro Leu Pro Asp Ile Ser Pro Asp Asn Lys Ile Leu Phe Ile 
        290                 295                 300             


Ala Gly His Asp Thr Asn Ile Ala Asn Ile Ser Gly Met Leu Gly Met 
    305                 310                 315                 


Thr Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Ala 
320                 325                 330                 335 


Leu Val Phe Glu Arg Trp Val Asp Asn Ala Gly Lys Pro Tyr Val Ser 
                340                 345                 350     


Val Asn Met Val Tyr Gln Thr Leu Ala Gln Leu His Asp Gln Ala Pro 
            355                 360                 365         


Leu Thr Leu Gln His Pro Ala Gly Ser Val Arg Leu Asn Ile Pro Gly 
        370                 375                 380             


Cys Ser Asp Gln Thr Pro Asp Gly Tyr Cys Pro Leu Ser Thr Phe Ser 
    385                 390                 395                 


Arg Leu Val Ser His Ser Val Glu Pro Ala Cys Gln Leu Pro 
400                 405                 410             


