                         SEQUENCE LISTING

<110>  University of Washington
       University of Alabama Research Foundation
       Gundlach, Jens H.
       Niederweis, Michael
       Butler, Thomas Z.
       Pavlenok, Mikhail
       Troll, Mark
 
<120>  MSP Nanopores and Related Methods

<130>  UWOTL-1-33161

<150>  US 61/098,938
<151>  2008-09-22

<160>  52    

<170>  PatentIn version 3.5

<210> 1
<211> 184
<212> PRT
<213> Mycobacteria Smegmatis

<400> 1
Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu
 1               5                  10                  15      
Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp
            20                  25                  30          
Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr
        35                  40                  45              
Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu
    50                  55                  60                  
Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe
65                  70                  75                  80  
Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Ala
                85                  90                  95      
Pro Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly
            100                 105                 110         
Val Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val
        115                 120                 125             
Ala Thr Phe Ser Val Asp Val Ser Gly Ala Glu Gly Gly Val Ala Val
    130                 135                 140                 
Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu
145                 150                 155                 160 
Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr
                165                 170                 175     
Tyr Gly Glu Pro Trp Asn Met Asn
            180                 


<210> 2
<211> 184
<212> PRT
<213> Mycobacteria Smegmatis

<400> 2
Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu
 1               5                  10                  15      
Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp
            20                  25                  30          
Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr
        35                  40                  45              
Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu
    50                  55                  60                  
Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe
65                  70                  75                  80  
Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Ala
                85                  90                  95      
Pro Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly
            100                 105                 110         
Val Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val
        115                 120                 125             
Ala Thr Phe Ser Val Asp Val Ser Gly Pro Ala Gly Gly Val Ala Val
    130                 135                 140                 
Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu
145                 150                 155                 160 
Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr
                165                 170                 175     
Tyr Gly Glu Pro Trp Asn Met Asn
            180                 


<210> 3
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> Linker Peptide between MspA Peptides

<400> 3
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
 1               5                  10                  15  


<210> 4
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 4
gctgttgctc tctcgcaaca gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 60
aaaaaaaaaa aa                                                     72


<210> 5
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 5
gctctgttgc tctctcgcaa cagagcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 60
aaaaaaaaaa aaaaaa                                                 76


<210> 6
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 6
gctgtctgtt gctctctcgc aacagacagc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 60
aaaaaaaaaa aaaaaaaaaa                                             80


<210> 7
<211> 75
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligonucleotides

<400> 7
ccccccaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 60
ctctattctt atctc                                                  75


<210> 8
<211> 75
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 8
ccccccaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 60
cacacacaca cacac                                                  75


<210> 9
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 9
gagataagaa tagag                                                  15


<210> 10
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 10
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa            50


<210> 11
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 11
aaaaaacccc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa            50


<210> 12
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 12
aaaccccaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa            50


<210> 13
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 13
aacaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa            50


<210> 14
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 14
caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa            50


<210> 15
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 15
ccccaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa            50


<210> 16
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 16
cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc            50


<210> 17
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 17
tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt            50


<210> 18
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 18
gggaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa            50


<210> 19
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> Oligonucleotides

<400> 19
ggcggtggcg gtagcggcgg tggcggtagc ggcggtggcg gtagc                 45


<210> 20
<211> 549
<212> DNA
<213> Mycobacteria Smegmatis

<400> 20
ggcctggaca acgagctgag cctcgttgat ggccaggacc gcaccctcac cgtgcagcag 60
tgggacacct tcctcaatgg tgtgttcccc ctggaccgca accgtcttac ccgtgagtgg 120
ttccactccg gtcgcgccaa gtacatcgtg gccggccccg gtgccgacga gttcgagggc 180
acgctggaac tcggctacca gatcggcttc ccgtggtcgc tgggtgtggg catcaacttc 240
agctacacca ccccgaacat cctgatcgac gacggtgaca tcaccgctcc gccgttcggc 300
ctgaactcgg tcatcacccc gaacctgttc cccggtgtgt cgatctcggc agatctgggc 360
aacggccccg gcatccagga agtcgcaacg ttctcggtcg acgtctccgg cgccgagggt 420
ggcgtggccg tgtcgaacgc ccacggcacc gtgaccggtg cggccggcgg tgtgctgctg 480
cgtccgttcg cccgcctgat cgcctcgacc ggtgactcgg tcaccaccta cggcgaaccc 540
tggaacatg                                                         549


<210> 21
<211> 549
<212> DNA
<213> Mycobacteria Smegmatis

<220> 
<223> Mutant MspA Gene

<400> 21
ggcctagaca atgagcttag ccttgttgac ggccaagacc gaaccctgac cctccagcaa 60
tgggatacct ttctcaacgg tgtattcccg ctggatcgca atcgtctaac ccgcgagtgg 120
ttccattccg gccgcgctaa gtatatcgta gccggacccg gggccgatga gtttgaggga 180
acgctcgaac ttggctatca gataggcttt ccgtggtcgc taggtgtcgg cattaacttt 240
agctatacca caccgaatat cctcatcgat gacggcgaca taaccgcgcc gcctttcggt 300
ctgaattcgg taatcacgcc gaatctgttt cccggggtgt ctatctcagc agacctgggg 360
aacggtcccg gaatccaaga agtggcaact ttctccgtcg atgtctcggg cgcggagggc 420
ggcgtagccg tctcgaatgc ccatggcacg gtgacaggtg cagccggggg tgtactgcta 480
cgtccattcg cgcgcctaat cgcgtcgaca ggtgattcgg ttaccacata cggtgaacca 540
tggaatatg                                                         549


<210> 22
<211> 549
<212> DNA
<213> Mycobacteria Smegmatis

<220> 
<223> Mutant MspA Gene

<400> 22
ggactggata acgaactgag tctggtagat gttcaggatc gcaatctcac agtgcaacag 60
tgggacactt tcctgaatgg cgtgtttccc ctcgaccgga accgacttac gcgtgaatgg 120
tttcactcag gtcgtgccaa atacattgtg gcgggcccgg gtgcggacga attcgaaggc 180
accctggagc tcgggtacca aatcggtttc ccttggtccc tgggcgtggg tatcaatttc 240
agttacacga cccccaacat tctgatagac gatggtgata tcacggctcc cccgtttggc 300
ctcaactccg tcataacccc caacctcttc cctggtgtct cgatttcggc cgatctcggc 360
aatggccctg gcattcagga ggtcgctacg ttttcggtgg acgtgtccgg tgccgaaggt 420
ggtgtggcgg tgtccaacgc gcacggtacc gtcaccggcg cggcgggcgg cgtgctcctg 480
cggccgtttg cccggctgat tgcctccacc ggggactccg tcacgaccta tggcgagccc 540
tggaacatg                                                         549


<210> 23
<211> 549
<212> DNA
<213> Mycobacteria Smegmatis

<220> 
<223> Mutant MspA Gene

<400> 23
ggccttgaca acgagctaag cctagttgac ggccaggacc gtaccctaac cctacagcag 60
tgggatacct ttctcaatag cgtcttccca ctggaccgca atcgtctgac ccgagagtgg 120
ttccactccg ggcgcgcaaa gtatatcgtc gccggtcccg gtgccgacga gttcgagttc 180
acgcttgaac taggctacca gattggcttc ccgtggtcgc ttggtgttgg cataaacttc 240
agctacacca ctccgaacat cctaatcgac gacggggaca ttaccgcacc gccattcggg 300
ctgaactcgg tgatcacacc gaacctgttt cccggagtgt caatctccgc agatcagggt 360
aacggacccg ggatccagga agttgcaacc ttctcagtcg acgtctcagg cgcagagggg 420
ggcgtcgccg tatcgaacgc ccacggcact gtgacgggtg ccgccggtgg tgtcctgctc 480
cgtcccttcg cacgcctcat cgcatcgacg ggtgactcgg taaccacgta cggggaaccg 540
tggaacatg                                                         549


<210> 24
<211> 549
<212> DNA
<213> Mycobacteria Smegmatis

<220> 
<223> Mutant MspA Gene

<400> 24
gggctggata acgaactgag tctcctggat ggacaggatc gcaatctcac ggtgcagcag 60
tgggacacat tccttaatgg ggtgttcccc cttgaccgta accggcttac acgtgaatgg 120
ttccactcgg gtcgggccaa gtacatagtg gctggccctg gtgctgacga gttcgaaggc 180
acactggaac tcggttacca aatcgggttc ccctggtcac tgggggtggg aatcaacttc 240
agctacacaa cccctaacat actgatagac gacggtgaca tcactgctcc tccgtttggc 300
cttaactcag tcattacccc taacctattc cccggtgttt cgatatcggc ggatcttggc 360
aacggcccgg gcatacagga agtcgcgacg ttctcggttg acgtttccgg ggccgagggt 420
ggggtggcag tgtcaaacgc tcacgggacc gttaccgggg cggcaggcgg ggtgcttctg 480
cgcccgttcg cccgtctgat agcctcaacc ggcgactcag tcacaaccta cggcgagccc 540
tggaacatg                                                         549


<210> 25
<211> 549
<212> DNA
<213> Mycobacteria Smegmatis

<220> 
<223> Mutant MspA Gene

<400> 25
ggactcgata atgaactcag tctagtagac ggtcaagatc ggaatctgac agtccaacaa 60
tgggatactt ttctgaacgg cgtctttccg ctcgatcgga atcgactcac gcgcgaatgg 120
tttcattcag ggcgtgcgaa atatattgtc gcggggccgg gcgcggatga atttgaaggt 180
accctcgagc tggggtatca aattggtttt ccttggtccc ttggcgtcgg tattaatttt 240
agttatacga cgcccaatat tctcatagat gatggcgata taacggcgcc cccatttggg 300

ctcaattccg tgataacgcc caatctcttt cctggcgtct ccatttccgc cgacctcggt 360
aatggtcctg gtattcaaga ggtggctacc ttttccgtgg atgtgtcggg tgcggaaggc 420
ggtgtagcgg tatccaatgc gcatggtaca gtcacaggcg ccgcgggtgg cgtcctcctc 480
cggccatttg cacggctaat tgcgtccacg ggggattccg tgacgacgta tggtcagccg 540
tggaacatg                                                         549


<210> 26
<211> 549
<212> DNA
<213> Mycobacteria Smegmatis

<220> 
<223> Mutant MspA Gene

<400> 26
gggctagata atcagcttag tcttgtggac gggcaagatc gaacgctgac gctccaacaa 60
tgggatacgt ttctaaacgg cgtatttccg ctcgatcgga atcgactaac gcgcgaatgg 120
tttcattcgg gccgagctaa atatatagta gcaggaccgg gggcagatga atttgaagga 180
acactcgagc ttgggtatca aataggtttt ccttggtccc taggagtcgg gattaatttt 240
agttatacaa cacccaatat actcattgat gatggcgata taacggcgcc tccttttggt 300
ctcaattccg taattacgcc taatctcttt ccgggggtgt ctatatcagc cgacctcggg 360
aacggtccag gaattcaaga ggtggcgact ttttccgtgg atgtttcggg agcggaaggc 420
ggtgtagcag cgtccaatgc tcatggaacg gtcacaggcg cagcaacagg agtactccca 480
cggccatttg cgcggctaat tgcgtctaca ggcgattccg ttacgacata tggtgagcca 540
tggaatatg                                                         549


<210> 27
<211> 549
<212> DNA
<213> Mycobacteria Smegmatis

<220> 
<223> Mutant MspA Gene

<400> 27
ggacttgata acgaactaag tctggtagat ggtcaggatc gtaatcttac agttcaacag 60
tgggacactt tcctgaatgg cgtttttcct ctcgaccgga accgactgac gcgggaatgg 120
tttcactcag gccgtgcaaa atacaatgta gcgggtccgg gagcggacga attcgaaggg 180
accctagagc ttgggtacca aatgggtttc ccttggtccc tcggcgtagg tataaatttc 240
agttacacga cacccaacat tcttatagac gatggggata ttacggcacc cccctttggt 300
ctcaactccg taataacacc caacctcttc cctggagtct ctatttcagc cgatctcgga 360
aatggacctg gaattcagga ggttgctaca ttttctgtgg acgtgtcagg tgcagaagga 420
ggtgtcgcgg tctccaacgc gcacggtacg gtcacgggcg ctgcgggggg cgttctcctt 480
cggccctttg cgcggctcat tgcttccaca ggggactccg taacgactta tggacagcct 540
tggaatatg                                                         549


<210> 28
<211> 211
<212> PRT
<213> Mycobacteria Smegmatis

<220> 
<223> MspA Protein 

<400> 28
Met Lys Ala Ile Ser Arg Val Leu Ile Ala Met Val Ala Ala Ile Ala
 1               5                  10                  15      
Ala Leu Phe Thr Ser Thr Gly Thr Ser His Ala Gly Leu Asp Asn Glu
            20                  25                  30          
Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu Thr Val Gln Gln Trp
        35                  40                  45              
Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp Arg Asn Arg Leu Thr
    50                  55                  60                  
Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr Ile Val Ala Gly Pro
65                  70                  75                  80  
Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu Gly Tyr Gln Ile Gly
                85                  90                  95      
Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe Ser Tyr Thr Thr Pro
            100                 105                 110         
Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Ala Pro Pro Phe Gly Leu
        115                 120                 125             
Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly Val Ser Ile Ser Ala
    130                 135                 140                 
Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val Ala Thr Phe Ser Val
145                 150                 155                 160 
Asp Val Ser Gly Ala Glu Gly Gly Val Ala Val Ser Asn Ala His Gly
                165                 170                 175     
Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu Arg Pro Phe Ala Arg
            180                 185                 190         
Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr Tyr Gly Glu Pro Trp
        195                 200                 205             
Asn Met Asn
    210     


<210> 29
<211> 215
<212> PRT
<213> Mycobacteria Smegmatis

<220> 
<223> MspB Protein 

<400> 29
Met Thr Ala Phe Lys Arg Val Leu Ile Ala Met Ile Ser Ala Leu Leu
 1               5                  10                  15      
Ala Gly Thr Thr Gly Met Phe Val Ser Ala Gly Ala Ala His Ala Gly
            20                  25                  30          
Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu Thr
        35                  40                  45              
Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp Arg
    50                  55                  60                  
Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr Ile
65                  70                  75                  80  
Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu Gly
                85                  90                  95      
Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe Ser
            100                 105                 110         
Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Ala Pro
        115                 120                 125             
Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly Val
    130                 135                 140                 
Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val Ala
145                 150                 155                 160 
Thr Phe Ser Val Asp Val Ser Gly Pro Ala Gly Gly Val Ala Val Ser
                165                 170                 175     
Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu Arg
            180                 185                 190         
Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr Tyr
        195                 200                 205             
Gly Glu Pro Trp Asn Met Asn
    210                 215 


<210> 30
<211> 215
<212> PRT
<213> Mycobacteria Smegmatis

<220> 
<223> MspC Protein 

<400> 30
Met Lys Ala Ile Ser Arg Val Leu Ile Ala Met Ile Ser Ala Leu Ala
 1               5                  10                  15      
Ala Ala Val Ala Gly Leu Phe Val Ser Ala Gly Thr Ser His Ala Gly
            20                  25                  30          
Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu Thr
        35                  40                  45              
Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp Arg
    50                  55                  60                  
Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr Ile
65                  70                  75                  80  
Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu Gly
                85                  90                  95      
Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe Ser
            100                 105                 110         
Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Gly Pro
        115                 120                 125             
Pro Phe Gly Leu Glu Ser Val Ile Thr Pro Asn Leu Phe Pro Gly Val
    130                 135                 140                 
Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val Ala
145                 150                 155                 160 
Thr Phe Ser Val Asp Val Ser Gly Pro Ala Gly Gly Val Ala Val Ser
                165                 170                 175     
Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu Arg
            180                 185                 190         
Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr Tyr
        195                 200                 205             
Gly Glu Pro Trp Asn Met Asn
    210                 215 


<210> 31
<211> 207
<212> PRT
<213> Mycobacteria Smegmatis

<220> 
<223> MspD Protein 

<400> 31
Met Arg Tyr Leu Val Met Met Phe Ala Leu Leu Val Ser Val Thr Leu
 1               5                  10                  15      
Val Ser Pro Arg Pro Ala Asn Ala Val Asp Asn Gln Leu Ser Val Val
            20                  25                  30          
Asp Gly Gln Gly Arg Thr Leu Thr Val Gln Gln Ala Glu Thr Phe Leu
        35                  40                  45              
Asn Gly Val Phe Pro Leu Asp Arg Asn Arg Leu Thr Arg Glu Trp Phe
    50                  55                  60                  
His Ser Gly Arg Ala Thr Tyr His Val Ala Gly Pro Gly Ala Asp Glu
65                  70                  75                  80  
Phe Glu Gly Thr Leu Glu Leu Gly Tyr Gln Val Gly Phe Pro Trp Ser
                85                  90                  95      
Leu Gly Val Gly Ile Asn Phe Ser Tyr Thr Thr Pro Asn Ile Leu Ile
            100                 105                 110         
Asp Gly Gly Asp Ile Thr Gln Pro Pro Phe Gly Leu Asp Thr Ile Ile
        115                 120                 125             
Thr Pro Asn Leu Phe Pro Gly Val Ser Ile Ser Ala Asp Leu Gly Asn
    130                 135                 140                 
Gly Pro Gly Ile Gln Glu Val Ala Thr Phe Ser Val Asp Val Lys Gly
145                 150                 155                 160 
Ala Lys Gly Ala Val Ala Val Ser Asn Ala His Gly Thr Val Thr Gly
                165                 170                 175     
Ala Ala Gly Gly Val Leu Leu Arg Pro Phe Ala Arg Leu Ile Ala Ser
            180                 185                 190         
Thr Gly Asp Ser Val Thr Thr Tyr Gly Glu Pro Trp Asn Met Asn
        195                 200                 205         


<210>  32
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides


<400>  32
tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt                  50


<210>  33
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  33
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaca                  50


<210>  34
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  34
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaat                  50


<210>  35
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  35
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaata                  50


<210>  36
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  36
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaataa                  50


<210>  37
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  37
cccccccccc cccccccccc cccccccccc cccccccccc ccccccccca                  50


<210>  38
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  38
cccccccccc cccccccccc cccccccccc cccccccccc ccccccccac                  50


<210>  39
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  39
cccccccccc cccccccccc cccccccccc cccccccccc cccccccacc                  50


<210>  40
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  40
cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc cccccccc         58


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  41
cgaccagcac ggcatacatc                                                   20



<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  42
cgttctcggc tcgatgatcc                                                   20


<210>  43
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  43
cctgatcaac aacggtaaca tcaccgc                                           27


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  44
ctgggcacgc ctgggcaacg g                                                 21


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  45
tccggcgccc gcggtggcgt g                                                 21


<210>  46
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  46
ggcgccaagg gtggcgtgg                                                    19


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  47
cgttctcggt ccgcgtctcc                                                   20


<210>  48
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  48
cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc                  50


<210>  49
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  49
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa                  50


<210>  50
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  50
aacccaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa               52


<210>  51
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  51
tacgcataca tcctaagaac tcagactacc tcccaataaa tccacac                     47


<210>  52
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotides

<400>  52
tcagactacc tcccaataaa tccgcagcaa tcctcacacc taataat                     47
