
                                SEQUENCE LISTING

<110> Calmune Corporation
      Williamson, Robert Anthony
      Wadia, Jehangir
      Maruyama, Toshiaki
      Chen, Zhifeng
      Nelson, Joshua

<120> METHODS FOR CREATING DIVERSITY IN
  LIBRARIES AND LIBRARIES, DISPLAY VECTORS AND METHODS, AND
  DISPLAYED MOLECULES

<130> 3800013-00032/1106PC

<140> Not yet assigned
<141> Herewith

<150> 61/192,916
<151> 2008-09-22

<160> 299

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 35
<212> PRT
<213> Artificial Sequence

<220> 
<223> GCN4 zipper

<400> 1
Gly Arg Met Lys Gln Leu Glu Asp Lys Val Glu Glu Leu Leu Ser Lys
 1               5                  10                  15      
Asn Tyr His Leu Glu Asn Glu Val Ala Arg Leu Lys Lys Leu Val Gly
            20                  25                  30          
Glu Arg Gly
        35  


<210> 2
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> SAP-1 cleavage site

<400> 2
gctcttc                                                           7

<210> 3
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> CALX24 Primer

<400> 3
gccgctgtgc catcgctcag taac                                        24

<210> 4
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCALVH-F Primer

<400> 4
gcccaggcgg ccgcagaagt tcagctggtt gaatctggtg                       40

<210> 5
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer E / oligonucleotide pool E

<400> 5
cctttggtcg acgccggaga aacggtaaca acggtacccg gaccccaagc gtcgaacg   58

<210> 6
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> CALX24H1S-F primer F1

<400> 6
gccgctgtgc catcgctcag taacgcggcc gcagaagttc agctg                 45

<210> 7
<211> 3513
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL G13 vector

<400> 7
gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 60
caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 120
ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 180
gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 240
tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 300
ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 360
tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 420
atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 480
gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 540
caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 600
ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 660
ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 720
ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 780
ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 840
gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 900
ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 960
taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1020
agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1080
atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1140
aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200
caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1260
ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1320
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1380
tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1440
gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1500
ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1560
gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1620
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1680
ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1740
tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1800
ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1860
agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1920
aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1980
gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 2040
tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2100
tgtgtggaat tgtgagcgga taacaattga attaaggagg atataattat gaaatacctg 2160
ctgccgaccg cagccgctgg tctgctgctg ctcgcggccc agccggccat ggccgccggt 2220
gcctaactct ggctggtttc gctaccgtaa ccggtttaat taataaggag gatataatta 2280
tgaaaaagac agctatcgcg attgcagtgg cactggctgg tttcgctacc gtagcccagg 2340
cggccgcacg cgtctggttg aatctggtgg ggtctggaat tctgcgatcg cggccaggcc 2400
ggccgcacca tcaccatcac catggcgcat acccgtacga cgttccggac tacgcttcta 2460
ctagttagga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag 2520
gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta 2580
ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca 2640
aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt 2700
ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg 2760
ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt 2820
ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cgctggtaaa ccatatgaat 2880
tttctattga ttgtgacaaa ataaacttat tccgtggtgt ctttgcgttt cttttatatg 2940
ttgccacctt tatgtatgta ttttctacgt ttgctaacat actgcgtaat aaggagtctt 3000
aagctagcta acgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg 3060
ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 3120
cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 3180
gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 3240
ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 3300
ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 3360
ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 3420
attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 3480
aattttaaca aaatattaac gcttacaatt tag                              3513

<210> 8
<211> 3513
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL A1 vector

<400> 8
gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 60
caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 120
ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 180
gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 240
tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 300
ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 360
tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 420
atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 480
gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 540
caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 600
ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 660
ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 720
ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 780
ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 840
gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 900
ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 960
taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1020
agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1080
atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1140
aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200
caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1260
ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1320
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1380
tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1440
gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1500
ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1560
gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1620
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1680
ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1740
tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1800
ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1860
agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1920
aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1980
gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 2040
tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2100
tgtgtggaat tgtgagcgga taacaattga attaaggagg atataattat gaaatacctg 2160
ctgccgaccg cagccgctgg tctgctgctg ctcgcggccc agccggccat ggccgccggt 2220
gcctaactct ggctggtttc gctaccgtaa ccggtttaat taataaggag gatataatta 2280
tgaaaaagac agctatcgcg attgcagtgg cactggctgg tttcgctacc gtagcccagg 2340
cggccgcacg cgtctggttg aatctggtgg ggtctggaat tctgcgatcg cggccaggcc 2400
ggccgcacca tcaccatcac catggcgcat acccgtacga cgttccggac tacgcttcta 2460
ctagttagaa gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag 2520
gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta 2580
ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca 2640
aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt 2700
ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg 2760
ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt 2820
ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cgctggtaaa ccatatgaat 2880
tttctattga ttgtgacaaa ataaacttat tccgtggtgt ctttgcgttt cttttatatg 2940
ttgccacctt tatgtatgta ttttctacgt ttgctaacat actgcgtaat aaggagtctt 3000
aagctagcta acgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg 3060
ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 3120
cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 3180
gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 3240
ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 3300
ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 3360
ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 3420
attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 3480
aattttaaca aaatattaac gcttacaatt tag                              3513

<210> 9
<211> 3
<212> DNA
<213> Artificial Sequence

<220> 
<223> amber stop DNA

<400> 9
tag                                                               3

<210> 10
<211> 3
<212> RNA
<213> Artificial Sequence

<220> 
<223> amber stop RNA

<400> 10
uag                                                               3

<210> 11
<211> 4765
<212> DNA
<213> Artificial Sequence

<220> 
<223> 2G12 pCAL G13 vector

<400> 11
gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 60
caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 120
ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 180
gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 240
tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 300
ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 360
tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 420
atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 480
gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 540
caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 600
ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 660
ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 720
ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 780
ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 840
gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 900
ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 960
taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1020
agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1080
atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1140
aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200
caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1260
ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1320
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1380
tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1440
gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1500
ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1560
gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1620
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1680
ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1740
tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1800
ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1860
agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1920
aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1980
gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 2040
tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2100
tgtgtggaat tgtgagcgga taacaattga attaaggagg atataattat gaaatacctg 2160
ctgccgaccg cagccgctgg tctgctgctg ctcgcggccc agccggccat ggccgccggt 2220
gttgttatga cccagtctcc gtctaccctg tctgcttctg ttggtgacac catcaccatc 2280
acctgccgtg cttctcagtc tatcgaaacc tggctggctt ggtaccagca gaaaccgggt 2340
aaagctccga aactgctgat ctacaaggct tctaccctga aaaccggtgt tccgtctcgt 2400
ttctctggtt ctggttctgg taccgagttc accctgacca tctctggtct gcagttcgac 2460
gacttcgcta cctaccactg ccagcactac gctggttact ctgctacctt cggtcagggt 2520
acccgtgttg aaatcaaacg taccgttgct gctccgtctg ttttcatctt cccgccgtct 2580
gacgaacagc tgaaatctgg taccgcttct gttgtttgcc tgctgaacaa cttctacccg 2640
cgtgaagcta aagttcagtg gaaagttgac aacgctctgc agtctggtaa ctctcaggaa 2700
tctgttaccg aacaggactc taaagactct acctactctc tgtcttctac cctgaccctg 2760
tctaaagctg actacgaaaa gcacaaagtt tacgcttgcg aagttaccca ccagggtctg 2820
tcttctccgg ttaccaaatc tttcaaccgt ggtgaatgct aattaattaa taaggaggat 2880
ataattatga aaaagacagc tatcgcgatt gcagtggcac tggctggttt cgctaccgta 2940
gcccaggcgg ccgcagaagt tcagctggtt gaatctggtg gtggtctggt taaagctggt 3000
ggttctctga tcctgtcttg cggtgtttct aacttccgta tctctgctca caccatgaac 3060
tgggttcgtc gtgttccggg tggtggtctg gaatgggttg cttctatctc tacctcttct 3120
acctaccgtg actacgctga cgctgttaaa ggtcgtttca ccgtttctcg tgacgacctg 3180
gaagacttcg tttacctgca gatgcataaa atgcgtgttg aagacaccgc tatctactac 3240
tgcgctcgta aaggttctga ccgtctgtct gacaacgacc cgttcgacgc ttggggtccg 3300
ggtaccgttg ttaccgtttc tccggcgtcg accaaaggtc cgtctgtttt cccgctggct 3360
ccgtcttcta aatctacctc tggtggtacc gctgctctgg gttgcctggt taaagactac 3420
ttcccggaac cggttaccgt ttcttggaac tctggtgctc tgacctctgg tgttcacacc 3480
ttcccggctg ttctgcagtc ttctggtctg tactctctgt cttctgttgt taccgttccg 3540
tcttcttctc tgggtaccca gacctacatc tgcaacgtta accacaaacc gtctaacacc 3600
aaagttgaca agaaagttga accgaaatct tgcctgcgat cgcggccagg ccggccgcac 3660
catcaccatc accatggcgc atacccgtac gacgttccgg actacgcttc tactagttag 3720
gagggtggtg gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc 3780
ggtggtggct ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg 3840
gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat 3900
tctgtcgcta ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt 3960
gctaatggta atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc 4020
ggtgacggtg ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct 4080
caatcggttg aatgtcgccc ttttgtcttt ggcgctggta aaccatatga attttctatt 4140
gattgtgaca aaataaactt attccgtggt gtctttgcgt ttcttttata tgttgccacc 4200
tttatgtatg tattttctac gtttgctaac atactgcgta ataaggagtc ttaagctagc 4260
taacgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag 4320
cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 4380
cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 4440
tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 4500
cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 4560
gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 4620
aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 4680
gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 4740
caaaatatta acgcttacaa tttag                                       4765

<210> 12
<211> 424
<212> PRT
<213> Artificial Sequence

<220> 
<223> M13 gene III protein (cp3, g3p, minor coat
      protein, g.i. 59799327)

<400> 12
Met Lys Lys Leu Leu Phe Ala Ile Pro Leu Val Val Pro Phe Tyr Ser
 1               5                  10                  15      
His Ser Ala Glu Thr Val Glu Ser Cys Leu Ala Lys Pro His Thr Glu
            20                  25                  30          
Asn Ser Phe Thr Asn Val Trp Lys Asp Asp Lys Thr Leu Asp Arg Tyr
        35                  40                  45              
Ala Asn Tyr Glu Gly Cys Leu Trp Asn Ala Thr Gly Val Val Val Cys
    50                  55                  60                  
Thr Gly Asp Glu Thr Gln Cys Tyr Gly Thr Trp Val Pro Ile Gly Leu
65                  70                  75                  80  
Ala Ile Pro Glu Asn Glu Gly Gly Gly Ser Glu Gly Gly Gly Ser Glu
                85                  90                  95      
Gly Gly Gly Ser Glu Gly Gly Gly Thr Lys Pro Pro Glu Tyr Gly Asp
            100                 105                 110         
Thr Pro Ile Pro Gly Tyr Thr Tyr Ile Asn Pro Leu Asp Gly Thr Tyr
        115                 120                 125             
Pro Pro Gly Thr Glu Gln Asn Pro Ala Asn Pro Asn Pro Ser Leu Glu
    130                 135                 140                 
Glu Ser Gln Pro Leu Asn Thr Phe Met Phe Gln Asn Asn Arg Phe Arg
145                 150                 155                 160 
Asn Arg Gln Gly Ala Leu Thr Val Tyr Thr Gly Thr Val Thr Gln Gly
                165                 170                 175     
Thr Asp Pro Val Lys Thr Tyr Tyr Gln Tyr Thr Pro Val Ser Ser Lys
            180                 185                 190         
Ala Met Tyr Asp Ala Tyr Trp Asn Gly Lys Phe Arg Asp Cys Ala Phe
        195                 200                 205             
His Ser Gly Phe Asn Glu Asp Pro Phe Val Cys Glu Tyr Gln Gly Gln
    210                 215                 220                 
Ser Ser Asp Leu Pro Gln Pro Pro Val Asn Ala Gly Gly Gly Ser Gly
225                 230                 235                 240 
Gly Gly Ser Gly Gly Gly Ser Glu Gly Gly Gly Ser Glu Gly Gly Gly
                245                 250                 255     
Ser Glu Gly Gly Gly Ser Glu Gly Gly Gly Ser Gly Gly Gly Ser Gly
            260                 265                 270         
Ser Gly Asp Phe Asp Tyr Glu Lys Met Ala Asn Ala Asn Lys Gly Ala
        275                 280                 285             
Met Thr Glu Asn Ala Asp Glu Asn Ala Leu Gln Ser Asp Ala Lys Gly
    290                 295                 300                 
Lys Leu Asp Ser Val Ala Thr Asp Tyr Gly Ala Ala Ile Asp Gly Phe
305                 310                 315                 320 
Ile Gly Asp Val Ser Gly Leu Ala Asn Gly Asn Gly Ala Thr Gly Asp
                325                 330                 335     
Phe Ala Gly Ser Asn Ser Gln Met Ala Gln Val Gly Asp Gly Asp Asn
            340                 345                 350         
Ser Pro Leu Met Asn Asn Phe Arg Gln Tyr Leu Pro Ser Leu Pro Gln
        355                 360                 365             
Ser Val Glu Cys Arg Pro Phe Val Phe Ser Ala Gly Lys Pro Tyr Glu
    370                 375                 380                 
Phe Ser Ile Asp Cys Asp Lys Ile Asn Leu Phe Arg Gly Val Phe Ala
385                 390                 395                 400 
Phe Leu Leu Tyr Val Ala Thr Phe Met Tyr Val Phe Ser Thr Phe Ala
                405                 410                 415     
Asn Ile Leu Arg Asn Lys Glu Ser
            420                 


<210> 13
<211> 123
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2G12 VH domain

<400> 13
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Ala Gly Gly
 1               5                  10                  15      
Ser Leu Ile Leu Ser Cys Gly Val Ser Asn Phe Arg Ile Ser Ala His
            20                  25                  30          
Thr Met Asn Trp Val Arg Arg Val Pro Gly Gly Gly Leu Glu Trp Val
        35                  40                  45              
Ala Ser Ile Ser Thr Ser Ser Thr Tyr Arg Asp Tyr Ala Asp Ala Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Val Ser Arg Asp Asp Leu Glu Asp Phe Val Tyr
65                  70                  75                  80  
Leu Gln Met His Lys Met Arg Val Glu Asp Thr Ala Ile Tyr Tyr Cys
                85                  90                  95      
Ala Arg Lys Gly Ser Asp Arg Leu Ser Asp Asn Asp Pro Phe Asp Ala
            100                 105                 110         
Trp Gly Pro Gly Thr Val Val Thr Val Ser Pro
        115                 120             


<210> 14
<211> 107
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2G12 VL domain (also 3-ALA VL) 1

<400> 14
Asp Val Val Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Thr Ile Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Glu Thr Trp
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45              
Tyr Lys Ala Ser Thr Leu Lys Thr Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Gly Leu Gln Phe
65                  70                  75                  80  
Asp Asp Phe Ala Thr Tyr His Cys Gln His Tyr Ala Gly Tyr Ser Ala
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Arg Val Glu Ile Lys
            100                 105         


<210> 15
<211> 123
<212> PRT
<213> Artificial Sequence

<220> 
<223> 3-ALA 2G12 VH domain

<400> 15
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Ala Gly Gly
 1               5                  10                  15      
Ser Leu Ile Leu Ser Cys Gly Val Ser Asn Phe Arg Ile Ser Ala His
            20                  25                  30          
Thr Met Asn Trp Val Arg Arg Val Pro Gly Gly Gly Leu Glu Trp Val
        35                  40                  45              
Ala Ser Ile Ser Thr Ser Ser Thr Tyr Arg Asp Tyr Ala Asp Ala Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Val Ser Arg Asp Asp Leu Glu Asp Phe Val Tyr
65                  70                  75                  80  
Leu Gln Met His Lys Met Arg Val Glu Asp Thr Ala Ile Tyr Tyr Cys
                85                  90                  95      
Ala Arg Lys Gly Ser Asp Arg Ala Ala Asp Ala Asp Pro Phe Asp Ala
            100                 105                 110         
Trp Gly Pro Gly Thr Val Val Thr Val Ser Pro
        115                 120             


<210> 16
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> Linker 1

<400> 16
ggtggttcgt ctggatcttc ctcctctggt ggcggtggct cgggcggtgg tggc       54

<210> 17
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> Linker 1

<400> 17
Gly Gly Ser Ser Gly Ser Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly
 1               5                  10                  15      
Gly Gly
        


<210> 18
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> Linker 2

<400> 18
ggaggatccg gcagcagcag cagcggcggc ggcggcggga gctccggcgg cgga       54

<210> 19
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> Linker 2

<400> 19
Gly Gly Ser Gly Ser Ser Ser Ser Gly Gly Gly Gly Gly Ser Ser Gly
 1               5                  10                  15      
Gly Gly
        


<210> 20
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> L216

<400> 20
ggaggatccg gcagcagcag cagcggcggc gggagctccg gcggcgga              48

<210> 21
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> L216

<400> 21
Gly Gly Ser Gly Ser Ser Ser Ser Gly Gly Gly Ser Ser Gly Gly Gly
 1               5                  10                  15      


<210> 22
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> L217

<400> 22
ggaggatccg gcagcagcag cagcggcggc ggcgggagct ccggcggcgg a          51

<210> 23
<211> 17
<212> PRT
<213> Artificial Sequence

<220> 
<223> L217

<400> 23
Gly Gly Ser Gly Ser Ser Ser Ser Gly Gly Gly Gly Ser Ser Gly Gly
 1               5                  10                  15      
Gly
    


<210> 24
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> L219

<400> 24
ggaggatcca gcggcagcag cagcagcggc ggcggcggcg ggagctccgg cggcgga    57

<210> 25
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> L219

<400> 25
Gly Gly Ser Ser Gly Ser Ser Ser Ser Gly Gly Gly Gly Gly Ser Ser
 1               5                  10                  15      
Gly Gly Gly
            


<210> 26
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> L220

<400> 26
ggaggatcca gcggcggcag cagcagcagc ggcggcggcg gcgggagctc cggcggcgga 60


<210> 27
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> L220

<400> 27
Gly Gly Ser Ser Gly Gly Ser Ser Ser Ser Gly Gly Gly Gly Gly Ser
 1               5                  10                  15      
Ser Gly Gly Gly
            20  


<210> 28
<211> 87
<212> DNA
<213> Artificial Sequence

<220> 
<223> BamHISacI linker / BamHISacI(+)

<400> 28
gatccggtgg cggcagcgaa ggtggtggca gcgaaggtgg cggtagcgaa ggtggcggca 60
gcgaaggcgg cggtagcggt gggagct                                     87

<210> 29
<211> 29
<212> PRT
<213> Artificial Sequence

<220> 
<223> BamHISacI linker

<400> 29
Asp Pro Val Ala Ala Ala Lys Val Val Ala Ala Lys Val Ala Val Ala
 1               5                  10                  15      
Lys Val Ala Ala Ala Lys Ala Ala Val Ala Val Gly Ala
            20                  25                  


<210> 30
<211> 6819
<212> DNA
<213> Artificial Sequence

<220> 
<223> vector with 2G12 Fab Cys19 construct

<400> 30
ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag 60
gagatatacc atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac 120
cgtggcccag gcggccgttg ttatgaccca gtctccgtct accctgtctg cttctgttgg 180
tgacaccatc accatcacct gccgtgcttc tcagtctatc gaaacctggc tggcttggta 240
ccagcagaaa ccgggtaaag ctccgaaact gctgatctac aaggcttcta ccctgaaaac 300
cggtgttccg tctcgtttct ctggttctgg ttctggtacc gagttcaccc tgaccatctc 360
tggtctgcag ttcgacgact tcgctaccta ccactgccag cactacgctg gttactctgc 420
taccttcggt cagggtaccc gtgttgaaat caaacgtacc gttgctgctc cgtctgtttt 480
catcttcccg ccgtctgacg aacagctgaa atctggtacc gcttctgttg tttgcctgct 540
gaacaacttc tacccgcgtg aagctaaagt tcagtggaaa gttgacaacg ctctgcagtc 600
tggtaactct caggaatctg ttaccgaaca ggactctaaa gactctacct actctctgtc 660
ttctaccctg accctgtcta aagctgacta cgaaaagcac aaagtttacg cttgcgaagt 720
tacccaccag ggtctgtctt ctccggttac caaatctttc aaccgtggtg aatgctaggg 780
ccaggccggc cgcggccgca taatgcttaa gtcgaacaga aagtaatcgt attgtacacg 840
gccgcataat cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc 900
ccatcttagt atattagtta agtataagaa ggagatatac atatgaaata cctattgcct 960
acggcagccg ctggattgtt attactcgct gcccaaccag ccatggccga agttcagctg 1020
gttgaatctg gtggtggtct ggttaaagct ggtggttctc tgtgcctgtc ttgcggtgtt 1080
tctaacttcc gtatctctgc tcacaccatg aactgggttc gtcgtgttcc gggtggtggt 1140
ctggaatggg ttgcttctat ctctacctct tctacctacc gtgactacgc tgacgctgtt 1200
aaaggtcgtt tcaccgtttc tcgtgacgac ctggaagact tcgtttacct gcagatgcat 1260
aaaatgcgtg ttgaagacac cgctatctac tactgcgctc gtaaaggttc tgaccgtctg 1320
tctgacaacg acccgttcga cgcttggggt ccgggtaccg ttgttaccgt ttctccggcg 1380
tcgaccaaag gtccgtctgt tttcccgctg gctccgtctt ctaaatctac ctctggtggt 1440
accgctgctc tgggttgcct ggttaaagac tacttcccgg aaccggttac cgtttcttgg 1500
aactctggtg ctctgacctc tggtgttcac accttcccgg ctgttctgca gtcttctggt 1560
ctgtactctc tgtcttctgt tgttaccgtt ccgtcttctt ctctgggtac ccagacctac 1620
atctgcaacg ttaaccacaa accgtctaac accaaagttg acaagaaagt tgaaccgaaa 1680
tcttgcggca gcagccacca tcaccatcac catggcgcat acccgtacga cgttccggac 1740
tacgcttctt agctcgagtc tggtaaagaa accgctgctg cgaaatttga acgccagcac 1800
atggactcgt ctactagcgc agcttaatta acctaggctg ctgccaccgc tgagcaataa 1860
ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga 1920
actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg 1980
tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 2040
ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 2100
ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 2160
agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 2220
tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 2280
tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 2340
atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt 2400
ctggcggcac gatggcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 2460
aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 2520
atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 2580
ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 2640
tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 2700
agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 2760
taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 2820
tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 2880
cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 2940
ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 3000
tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 3060
tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 3120
cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 3180
tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 3240
gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 3300
tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 3360
atgttgaata ctcatactct tcctttttca atcatgattg aagcatttat cagggttatt 3420
gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggtcatgacc 3480
aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 3540
ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 3600
ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 3660
actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 3720
caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 3780
gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 3840
ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 3900
cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 3960
cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 4020
acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 4080
ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 4140
gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 4200
tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 4260
accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 4320
cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt 4380
gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc 4440
gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg 4500
acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 4560
catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc 4620
atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt 4680
gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt 4740
tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat 4800
gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg 4860
gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa 4920
aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag 4980
ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt 5040
ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga 5100
cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc 5160
agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgctagt 5220
catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg 5280
agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc 5340
tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 5400
aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca 5460
gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt 5520
gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt 5580
cttcggtatc gtcgtatccc actaccgaga tgtccgcacc aacgcgcagc ccggactcgg 5640
taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa 5700
cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc 5760
cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca 5820
gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac 5880
ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac 5940
tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag 6000
cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc 6060
gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca 6120
tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt 6180
gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc 6240
ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca 6300
ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct 6360
gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca 6420
ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt 6480
cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc 6540
agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg 6600
gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc 6660
atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca 6720
gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag 6780
atcgatctcg atcccgcgaa attaatacga ctcactata                        6819


<210> 31
<211> 6802
<212> DNA
<213> Artificial Sequence

<220> 
<223> vector with 2G12 scFab deltaC2Cys19 construct

<400> 31
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 60
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 120
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 180
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 240
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 300
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 360
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 420
acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 480
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 540
tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 600
tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 660
actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 720
gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 780
aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 840
agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 900
cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 960
aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1020
tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1080
tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1140
taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1200
ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1260
tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1320
tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1380
cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1440
cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1500
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1560
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1620
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1680
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1740
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1800
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1860
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1920
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1980
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2040
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2100
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2160
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2220
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2280
tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2340
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2400
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2460
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2520
gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2580
gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2640
aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2700
ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2760
acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2820
ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2880
tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2940
tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3000
cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3060
gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3120
ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3180
catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3240
ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3300
gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3360
gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3420
ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3480
atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3540
cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3600
tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3660
ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3720
aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3780
atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3840
cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3900
gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3960
tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4020
agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4080
gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4140
ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4200
catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4260
tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4320
tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4380
gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4440
ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4500
tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4560
catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4620
cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4680
tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4740
ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4800
ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4860
cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4920
gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4980
aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5040
ttttgtttaa ctttaagaag gagatatacc atgaaaaaga cagctatcgc gattgcagtg 5100
gcactggctg gtttcgctac cgtggcccag gcggccgttg ttatgaccca gtctccgtct 5160
accctgtctg cttctgttgg tgacaccatc accatcacct gccgtgcttc tcagtctatc 5220
gaaacctggc tggcttggta ccagcagaaa ccgggtaaag ctccgaaact gctgatctac 5280
aaggcttcta ccctgaaaac cggtgttccg tctcgtttct ctggttctgg ttctggtacc 5340
gagttcaccc tgaccatctc tggtctgcag ttcgacgact tcgctaccta ccactgccag 5400
cactacgctg gttactctgc taccttcggt cagggtaccc gtgttgaaat caaacgtacc 5460
gttgctgctc cgtctgtttt catcttcccg ccgtctgacg aacagctgaa atctggtacc 5520
gcttctgttg tttgcctgct gaacaacttc tacccgcgtg aagctaaagt tcagtggaaa 5580
gttgacaacg ctctgcagtc tggtaactct caggaatctg ttaccgaaca ggactctaaa 5640
gactctacct actctctgtc ttctaccctg accctgtcta aagctgacta cgaaaagcac 5700
aaagtttacg cttgcgaagt tacccaccag ggtctgtctt ctccggttac caaatctttc 5760
aaccgtggtg aatctggtgg tggatccggt ggcggcagcg aaggtggtgg cagcgaaggt 5820
ggcggtagcg aaggtggcgg cagcgaaggc ggcggtagcg gtgggagctc cggtgaagtt 5880
cagctggttg aatctggtgg tggtctggtt aaagctggtg gttctctgtg cctgtcttgc 5940
ggtgtttcta acttccgtat ctctgctcac accatgaact gggttcgtcg tgttccgggt 6000
ggtggtctgg aatgggttgc ttctatctct acctcttcta cctaccgtga ctacgctgac 6060
gctgttaaag gtcgtttcac cgtttctcgt gacgacctgg aagacttcgt ttacctgcag 6120
atgcacaaaa tgcgtgttga agacaccgct atctactact gcgctcgtaa aggttctgac 6180
cgtctgtctg acaacgaccc gttcgacgct tggggtccgg gtaccgttgt taccgtttct 6240
ccggcgtcga ccaaaggtcc gtctgttttc ccgctggctc cgtcttctaa atctacctct 6300
ggtggtaccg ctgctctggg ttgcctggtt aaagactact tcccggaacc ggttaccgtt 6360
tcttggaact ctggtgctct gacctctggt gttcacacct tcccggctgt tctgcagtct 6420
tctggtctgt actctctgtc ttctgttgtt accgttccgt cttcttctct gggtacccag 6480
acctacatct gcaacgttaa ccacaaaccg tctaacacca aagttgacaa gaaagttgaa 6540
ccgaaatctg gcagcagcgg ccaggccggc cagcaccatc accatcacca tggcgcatac 6600
ccgtacgacg ttccggacta cgcttcttag gcggccgcac tcgagcacca ccaccaccac 6660
cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct 6720
gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg 6780
aaaggaggaa ctatatccgg at                                          6802


<210> 32
<211> 6121
<212> DNA
<213> Artificial Sequence

<220> 
<223> vector with 2G12 scFv Cys19 construct

<400> 32
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 60
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 120
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 180
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 240
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 300
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 360
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 420
acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 480
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 540
tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 600
tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 660
actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 720
gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 780
aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 840
agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 900
cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 960
aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1020
tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1080
tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1140
taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1200
ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1260
tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1320
tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1380
cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1440
cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1500
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1560
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1620
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1680
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1740
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1800
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1860
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1920
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1980
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2040
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2100
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2160
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2220
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2280
tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2340
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2400
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2460
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2520
gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2580
gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2640
aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2700
ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2760
acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2820
ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2880
tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2940
tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3000
cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3060
gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3120
ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3180
catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3240
ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3300
gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3360
gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3420
ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3480
atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3540
cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3600
tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3660
ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3720
aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3780
atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3840
cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3900
gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3960
tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4020
agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4080
gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4140
ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4200
catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4260
tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4320
tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4380
gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4440
ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4500
tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4560
catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4620
cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4680
tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4740
ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4800
ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4860
cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4920
gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4980
aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5040
ttttgtttaa ctttaagaag gagatatacc atgaaaaaga cagctatcgc gattgcagtg 5100
gcactggctg gtttcgctac cgtggcccag gcggccgttg ttatgaccca gtctccgtct 5160
accctgtctg cttctgttgg tgacaccatc accatcacct gccgtgcttc tcagtctatc 5220
gaaacctggc tggcttggta ccagcagaaa ccgggtaaag ctccgaaact gctgatctac 5280
aaggcttcta ccctgaaaac cggtgttccg tctcgtttct ctggttctgg ttctggtacc 5340
gagttcaccc tgaccatctc tggtctgcag ttcgacgact tcgctaccta ccactgccag 5400
cactacgctg gttactctgc taccttcggt cagggtaccc gtgttgaaat caaaggtggt 5460
tcgtctggat cttcctcctc tggtggcggt ggctcgggcg gtggtggcga agttcagctg 5520
gttgaatctg gtggtggtct ggttaaagct ggtggttctc tgtgcctgtc ttgcggtgtt 5580
tctaacttcc gtatctctgc tcacaccatg aactgggttc gtcgtgttcc gggtggtggt 5640
ctggaatggg ttgcttctat ctctacctct tctacctacc gtgactacgc tgacgctgtt 5700
aaaggtcgtt tcaccgtttc tcgtgacgac ctggaagact tcgtttacct gcagatgcac 5760
aaaatgcgtg ttgaagacac cgctatctac tactgcgctc gtaaaggttc tgaccgtctg 5820
tctgacaacg acccgttcga cgcttggggt ccgggtaccg ttgttaccgt ttctccgggc 5880
caggccggcc agcaccatca ccatcaccat ggcgcatacc cgtacgacgt tccggactac 5940
gcttcttagg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac 6000
aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc 6060
cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga 6120
t                                                                 6121


<210> 33
<211> 4765
<212> DNA
<213> Artificial Sequence

<220> 
<223> 3-ALA 2G12 pCAL G13 vector

<400> 33
gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 60
caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 120
ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 180
gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 240
tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 300
ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 360
tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 420
atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 480
gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 540
caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 600
ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 660
ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 720
ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 780
ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 840
gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 900
ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 960
taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1020
agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1080
atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1140
aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200
caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1260
ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1320
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1380
tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1440
gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1500
ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1560
gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1620
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1680
ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1740
tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1800
ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1860
agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1920
aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1980
gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 2040
tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2100
tgtgtggaat tgtgagcgga taacaattga attaaggagg atataattat gaaatacctg 2160
ctgccgaccg cagccgctgg tctgctgctg ctcgcggccc agccggccat ggccgccggt 2220
gttgttatga cccagtctcc gtctaccctg tctgcttctg ttggtgacac catcaccatc 2280
acctgccgtg cttctcagtc tatcgaaacc tggctggctt ggtaccagca gaaaccgggt 2340
aaagctccga aactgctgat ctacaaggct tctaccctga aaaccggtgt tccgtctcgt 2400
ttctctggtt ctggttctgg taccgagttc accctgacca tctctggtct gcagttcgac 2460
gacttcgcta cctaccactg ccagcactac gctggttact ctgctacctt cggtcagggt 2520
acccgtgttg aaatcaaacg taccgttgct gctccgtctg ttttcatctt cccgccgtct 2580
gacgaacagc tgaaatctgg taccgcttct gttgtttgcc tgctgaacaa cttctacccg 2640
cgtgaagcta aagttcagtg gaaagttgac aacgctctgc agtctggtaa ctctcaggaa 2700
tctgttaccg aacaggactc taaagactct acctactctc tgtcttctac cctgaccctg 2760
tctaaagctg actacgaaaa gcacaaagtt tacgcttgcg aagttaccca ccagggtctg 2820
tcttctccgg ttaccaaatc tttcaaccgt ggtgaatgct aattaattaa taaggaggat 2880
ataattatga aaaagacagc tatcgcgatt gcagtggcac tggctggttt cgctaccgta 2940
gcccaggcgg ccgcagaagt tcagctggtt gaatctggtg gtggtctggt taaagctggt 3000
ggttctctga tcctgtcttg cggtgtttct aacttccgta tctctgctca caccatgaac 3060
tgggttcgtc gtgttccggg tggtggtctg gaatgggttg cttctatctc tacctcttct 3120
acctaccgtg actacgctga cgctgttaaa ggtcgtttca ccgtttctcg tgacgacctg 3180
gaagacttcg tttacctgca gatgcataaa atgcgtgttg aagacaccgc tatctactac 3240
tgcgctcgta aaggttctga ccgtgcggcg gacgcggacc cgttcgacgc ttggggtccg 3300
ggtaccgttg ttaccgtttc tccggcgtcg accaaaggtc cgtctgtttt cccgctggct 3360
ccgtcttcta aatctacctc tggtggtacc gctgctctgg gttgcctggt taaagactac 3420
ttcccggaac cggttaccgt ttcttggaac tctggtgctc tgacctctgg tgttcacacc 3480
ttcccggctg ttctgcagtc ttctggtctg tactctctgt cttctgttgt taccgttccg 3540
tcttcttctc tgggtaccca gacctacatc tgcaacgtta accacaaacc gtctaacacc 3600
aaagttgaca agaaagttga accgaaatct tgcctgcgat cgcggccagg ccggccgcac 3660
catcaccatc accatggcgc atacccgtac gacgttccgg actacgcttc tactagttag 3720
gagggtggtg gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc 3780
ggtggtggct ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg 3840
gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat 3900
tctgtcgcta ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt 3960
gctaatggta atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc 4020
ggtgacggtg ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct 4080
caatcggttg aatgtcgccc ttttgtcttt ggcgctggta aaccatatga attttctatt 4140
gattgtgaca aaataaactt attccgtggt gtctttgcgt ttcttttata tgttgccacc 4200
tttatgtatg tattttctac gtttgctaac atactgcgta ataaggagtc ttaagctagc 4260
taacgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag 4320
cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 4380
cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 4440
tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 4500
cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 4560
gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 4620
aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 4680
gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 4740
caaaatatta acgcttacaa tttag                                       4765

<210> 34
<211> 6840
<212> DNA
<213> Artificial Sequence

<220> 
<223> 2G12 Fab hinge in petDuet

<400> 34
ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag 60
gagatatacc atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac 120
cgtggcccag gcggccgttg ttatgaccca gtctccgtct accctgtctg cttctgttgg 180
tgacaccatc accatcacct gccgtgcttc tcagtctatc gaaacctggc tggcttggta 240
ccagcagaaa ccgggtaaag ctccgaaact gctgatctac aaggcttcta ccctgaaaac 300
cggtgttccg tctcgtttct ctggttctgg ttctggtacc gagttcaccc tgaccatctc 360
tggtctgcag ttcgacgact tcgctaccta ccactgccag cactacgctg gttactctgc 420
taccttcggt cagggtaccc gtgttgaaat caaacgtacc gttgctgctc cgtctgtttt 480
catcttcccg ccgtctgacg aacagctgaa atctggtacc gcttctgttg tttgcctgct 540
gaacaacttc tacccgcgtg aagctaaagt tcagtggaaa gttgacaacg ctctgcagtc 600
tggtaactct caggaatctg ttaccgaaca ggactctaaa gactctacct actctctgtc 660
ttctaccctg accctgtcta aagctgacta cgaaaagcac aaagtttacg cttgcgaagt 720
tacccaccag ggtctgtctt ctccggttac caaatctttc aaccgtggtg aatgctaggg 780
ccaggccggc cgcggccgca taatgcttaa gtcgaacaga aagtaatcgt attgtacacg 840
gccgcataat cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc 900
ccatcttagt atattagtta agtataagaa ggagatatac atatgaaata cctattgcct 960
acggcagccg ctggattgtt attactcgct gcccaaccag ccatggccga agttcagctg 1020
gttgaatctg gtggtggtct ggttaaagct ggtggttctc tgatcctgtc ttgcggtgtt 1080
tctaacttcc gtatctctgc tcacaccatg aactgggttc gtcgtgttcc gggtggtggt 1140
ctggaatggg ttgcttctat ctctacctct tctacctacc gtgactacgc tgacgctgtt 1200
aaaggtcgtt tcaccgtttc tcgtgacgac ctggaagact tcgtttacct gcagatgcat 1260
aaaatgcgtg ttgaagacac cgctatctac tactgcgctc gtaaaggttc tgaccgtctg 1320
tctgacaacg acccgttcga cgcttggggt ccgggtaccg ttgttaccgt ttctccggcg 1380
tcgaccaaag gtccgtctgt tttcccgctg gctccgtctt ctaaatctac ctctggtggt 1440
accgctgctc tgggttgcct ggttaaagac tacttcccgg aaccggttac cgtttcttgg 1500
aactctggtg ctctgacctc tggtgttcac accttcccgg ctgttctgca gtcttctggt 1560
ctgtactctc tgtcttctgt tgttaccgtt ccgtcttctt ctctgggtac ccagacctac 1620
atctgcaacg ttaaccacaa accgtctaac accaaagttg acaagaaagt tgaaccgaaa 1680
agctgcgata aaacccatac ctgcccgccg tgcccgcacc atcaccatca ccatggcgca 1740
tacccgtacg acgttccgga ctacgcttct tagctcgagt ctggtaaaga aaccgctgct 1800
gcgaaatttg aacgccagca catggactcg tctactagcg cagcttaatt aacctaggct 1860
gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg 1920
ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg 1980
gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 2040
ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 2100
cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 2160
tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 2220
cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 2280
ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 2340
tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 2400
aaatattaac gtttacaatt tctggcggca cgatggcatg agattatcaa aaaggatctt 2460
cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 2520
aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 2580
atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 2640
cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 2700
tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 2760
atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 2820
taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 2880
tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 2940
gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 3000
cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 3060
cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 3120
gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 3180
aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 3240
accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 3300
ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 3360
gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatcatgatt 3420
gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 3480
ataaacaaat aggtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3540
gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3600
tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 3660
ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 3720
ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 3780
gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 3840
ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 3900
tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 3960
ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 4020
agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 4080
agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 4140
gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 4200
tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 4260
accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 4320
gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt 4380
atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 4440
gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 4500
aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 4560
tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 4620
gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg 4680
ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa 4740
gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg 4800
atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt 4860
tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg 4920
gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga 4980
tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt 5040
gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca 5100
tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat 5160
cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga 5220
caggagcacg atcatgctag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa 5280
ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat 5340
tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 5400
aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt 5460
tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca 5520
gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg 5580
gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac 5640
caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg 5700
caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac 5760
cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga 5820
gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta 5880
acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt 5940
cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg 6000
ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt 6060
taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt 6120
cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag 6180
atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc 6240
caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca 6300
gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt 6360
tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg 6420
ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac 6480
cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac 6540
tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg 6600
aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata 6660
cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg 6720
atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg 6780
cgtccggcgt agaggatcga gatcgatctc gatcccgcga aattaatacg actcactata 6840



<210> 35
<211> 6121
<212> DNA
<213> Artificial Sequence

<220> 
<223> vector with 2G12 domain exchanged scFv

<400> 35
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 60
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 120
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 180
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 240
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 300
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 360
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 420
acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 480
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 540
tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 600
tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 660
actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 720
gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 780
aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 840
agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 900
cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 960
aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1020
tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1080
tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1140
taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1200
ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1260
tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1320
tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1380
cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1440
cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1500
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1560
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1620
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1680
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1740
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1800
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1860
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1920
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1980
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2040
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2100
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2160
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2220
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2280
tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2340
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2400
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2460
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2520
gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2580
gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2640
aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2700
ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2760
acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2820
ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2880
tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2940
tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3000
cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3060
gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3120
ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3180
catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3240
ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3300
gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3360
gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3420
ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3480
atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3540
cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3600
tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3660
ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3720
aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3780
atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3840
cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3900
gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3960
tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4020
agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4080
gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4140
ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4200
catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4260
tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4320
tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4380
gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4440
ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4500
tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4560
catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4620
cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4680
tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4740
ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4800
ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4860
cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4920
gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4980
aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5040
ttttgtttaa ctttaagaag gagatatacc atgaaaaaga cagctatcgc gattgcagtg 5100
gcactggctg gtttcgctac cgtggcccag gcggccgttg ttatgaccca gtctccgtct 5160
accctgtctg cttctgttgg tgacaccatc accatcacct gccgtgcttc tcagtctatc 5220
gaaacctggc tggcttggta ccagcagaaa ccgggtaaag ctccgaaact gctgatctac 5280
aaggcttcta ccctgaaaac cggtgttccg tctcgtttct ctggttctgg ttctggtacc 5340
gagttcaccc tgaccatctc tggtctgcag ttcgacgact tcgctaccta ccactgccag 5400
cactacgctg gttactctgc taccttcggt cagggtaccc gtgttgaaat caaaggtggt 5460
tcgtctggat cttcctcctc tggtggcggt ggctcgggcg gtggtggcga agttcagctg 5520
gttgaatctg gtggtggtct ggttaaagct ggtggttctc tgatcctgtc ttgcggtgtt 5580
tctaacttcc gtatctctgc tcacaccatg aactgggttc gtcgtgttcc gggtggtggt 5640
ctggaatggg ttgcttctat ctctacctct tctacctacc gtgactacgc tgacgctgtt 5700
aaaggtcgtt tcaccgtttc tcgtgacgac ctggaagact tcgtttacct gcagatgcac 5760
aaaatgcgtg ttgaagacac cgctatctac tactgcgctc gtaaaggttc tgaccgtctg 5820
tctgacaacg acccgttcga cgcttggggt ccgggtaccg ttgttaccgt ttctccgggc 5880
caggccggcc agcaccatca ccatcaccat ggcgcatacc cgtacgacgt tccggactac 5940
gcttcttagg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac 6000
aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc 6060
cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga 6120
t                                                                 6121


<210> 36
<211> 6916
<212> DNA
<213> Artificial Sequence

<220> 
<223> vector w/ 2G12 domain exchanged scFv tandem

<400> 36
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 60
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 120
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 180
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 240
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 300
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 360
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 420
acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 480
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 540
tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 600
tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 660
actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 720
gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 780
aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 840
agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 900
cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 960
aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1020
tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1080
tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1140
taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1200
ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1260
tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1320
tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1380
cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1440
cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1500
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1560
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1620
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1680
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1740
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1800
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1860
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1920
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1980
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2040
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2100
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2160
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2220
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2280
tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2340
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2400
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2460
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2520
gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2580
gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2640
aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2700
ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2760
acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2820
ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2880
tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2940
tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3000
cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3060
gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3120
ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3180
catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3240
ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3300
gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3360
gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3420
ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3480
atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3540
cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3600
tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3660
ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3720
aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3780
atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3840
cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3900
gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3960
tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4020
agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4080
gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4140
ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4200
catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4260
tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4320
tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4380
gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4440
ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4500
tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4560
catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4620
cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4680
tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4740
ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4800
ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4860
cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4920
gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4980
aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5040
ttttgtttaa ctttaagaag gagatatacc atgaaaaaga cagctatcgc gattgcagtg 5100
gcactggctg gtttcgctac cgtggcccag gcggccgttg ttatgaccca gtctccgtct 5160
accctgtctg cttctgttgg tgacaccatc accatcacct gccgtgcttc tcagtctatc 5220
gaaacctggc tggcttggta ccagcagaaa ccgggtaaag ctccgaaact gctgatctac 5280
aaggcttcta ccctgaaaac cggtgttccg tctcgtttct ctggttctgg ttctggtacc 5340
gagttcaccc tgaccatctc tggtctgcag ttcgacgact tcgctaccta ccactgccag 5400
cactacgctg gttactctgc taccttcggt cagggtaccc gtgttgaaat caaaggtggt 5460
tcgtctggat cttcctcctc tggtggcggt ggctcgggcg gtggtggcga agttcagctg 5520
gttgaatctg gtggtggtct ggttaaagct ggtggttctc tgatcctgtc ttgcggtgtt 5580
tctaacttcc gtatctctgc tcacaccatg aactgggttc gtcgtgttcc gggtggtggt 5640
ctggaatggg ttgcttctat ctctacctct tctacctacc gtgactacgc tgacgctgtt 5700
aaaggtcgtt tcaccgtttc tcgtgacgac ctggaagact tcgtttacct gcagatgcac 5760
aaaatgcgtg ttgaagacac cgctatctac tactgcgctc gtaaaggttc tgaccgtctg 5820
tctgacaacg acccgttcga cgcttggggt ccgggtaccg ttgttaccgt ttctccggga 5880
ggatccggca gcagcagcag cggcggcggc ggcgggagct ccggcggcgg agaagttcag 5940
ctggttgaat ctggtggtgg tctggttaaa gctggtggtt ctctgatcct gtcttgcggt 6000
gtttctaact tccgtatctc tgctcacacc atgaactggg ttcgtcgtgt tccgggtggt 6060
ggtctggaat gggttgcttc tatctctacc tcttctacct accgtgacta cgctgacgct 6120
gttaaaggtc gtttcaccgt ttctcgtgac gacctggaag acttcgttta cctgcagatg 6180
cacaaaatgc gtgttgaaga caccgctatc tactactgcg ctcgtaaagg ttctgaccgt 6240
ctgtctgaca acgacccgtt cgacgcttgg ggtccgggta ccgttgttac cgtttctccg 6300
ggtggttcgt ctggatcttc ctcctctggt ggcggtggct cgggcggtgg tggcgttgtt 6360
atgacccagt ctccgtctac cctgtctgct tctgttggtg acaccatcac catcacctgc 6420
cgtgcttctc agtctatcga aacctggctg gcttggtacc agcagaaacc gggtaaagct 6480
ccgaaactgc tgatctacaa ggcttctacc ctgaaaaccg gtgttccgtc tcgtttctct 6540
ggttctggtt ctggtaccga gttcaccctg accatctctg gtctgcagtt cgacgacttc 6600
gctacctacc actgccagca ctacgctggt tactctgcta ccttcggtca gggtacccgt 6660
gttgaaatca aaggccaggc cggccagcac catcaccatc accatggcgc atacccgtac 6720
gacgttccgg actacgcttc ttaggcggcc gcactcgagc accaccacca ccaccactga 6780
gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa 6840
taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga 6900
ggaactatat ccggat                                                 6916


<210> 37
<211> 6166
<212> DNA
<213> Artificial Sequence

<220> 
<223> vector w/ 2G12 domain exchanged scFv hinge(E)

<400> 37
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 60
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 120
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 180
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 240
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 300
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 360
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 420
acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 480
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 540
tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 600
tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 660
actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 720
gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 780
aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 840
agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 900
cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 960
aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1020
tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1080
tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1140
taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1200
ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1260
tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1320
tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1380
cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1440
cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1500
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1560
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1620
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1680
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1740
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1800
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1860
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1920
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1980
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2040
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2100
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2160
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2220
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2280
tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2340
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2400
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2460
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2520
gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2580
gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2640
aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2700
ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2760
acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2820
ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2880
tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2940
tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3000
cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3060
gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3120
ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3180
catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3240
ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3300
gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3360
gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3420
ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3480
atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3540
cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3600
tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3660
ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3720
aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3780
atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3840
cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3900
gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3960
tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4020
agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4080
gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4140
ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4200
catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4260
tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4320
tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4380
gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4440
ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4500
tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4560
catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4620
cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4680
tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4740
ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4800
ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4860
cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4920
gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4980
aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5040
ttttgtttaa ctttaagaag gagatatacc atgaaaaaga cagctatcgc gattgcagtg 5100
gcactggctg gtttcgctac cgtggcccag gcggccgttg ttatgaccca gtctccgtct 5160
accctgtctg cttctgttgg tgacaccatc accatcacct gccgtgcttc tcagtctatc 5220
gaaacctggc tggcttggta ccagcagaaa ccgggtaaag ctccgaaact gctgatctac 5280
aaggcttcta ccctgaaaac cggtgttccg tctcgtttct ctggttctgg ttctggtacc 5340
gagttcaccc tgaccatctc tggtctgcag ttcgacgact tcgctaccta ccactgccag 5400
cactacgctg gttactctgc taccttcggt cagggtaccc gtgttgaaat caaaggtggt 5460
tcgtctggat cttcctcctc tggtggcggt ggctcgggcg gtggtggcga agttcagctg 5520
gttgaatctg gtggtggtct ggttaaagct ggtggttctc tgatcctgtc ttgcggtgtt 5580
tctaacttcc gtatctctgc tcacaccatg aactgggttc gtcgtgttcc gggtggtggt 5640
ctggaatggg ttgcttctat ctctacctct tctacctacc gtgactacgc tgacgctgtt 5700
aaaggtcgtt tcaccgtttc tcgtgacgac ctggaagact tcgtttacct gcagatgcac 5760
aaaatgcgtg ttgaagacac cgctatctac tactgcgctc gtaaaggttc tgaccgtctg 5820
tctgacaacg acccgttcga cgcttggggt ccgggtaccg ttgttaccgt ttctccggaa 5880
ccgaaaagct gcgataaaac ccatacctgc ccgccgtgcc cgggccaggc cggccagcac 5940
catcaccatc accatggcgc atacccgtac gacgttccgg actacgcttc ttaggcggcc 6000
gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa 6060
gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa 6120
cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggat                6166


<210> 38
<211> 6163
<212> DNA
<213> Artificial Sequence

<220> 
<223> vector w/ 2G12 domain exchanged scFv hinge deltaE

<400> 38
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 60
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 120
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 180
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 240
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 300
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 360
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 420
acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 480
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 540
tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 600
tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 660
actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 720
gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 780
aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 840
agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 900
cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 960
aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1020
tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1080
tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1140
taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1200
ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1260
tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1320
tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1380
cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1440
cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1500
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1560
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1620
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1680
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1740
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1800
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1860
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1920
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1980
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2040
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2100
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2160
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2220
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2280
tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2340
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2400
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2460
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2520
gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2580
gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2640
aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2700
ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2760
acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2820
ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2880
tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2940
tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3000
cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3060
gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3120
ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3180
catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3240
ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3300
gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3360
gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3420
ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3480
atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3540
cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3600
tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3660
ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3720
aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3780
atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3840
cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3900
gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3960
tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4020
agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4080
gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4140
ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4200
catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4260
tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4320
tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4380
gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4440
ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4500
tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4560
catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4620
cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4680
tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4740
ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4800
ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4860
cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4920
gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4980
aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5040
ttttgtttaa ctttaagaag gagatatacc atgaaaaaga cagctatcgc gattgcagtg 5100
gcactggctg gtttcgctac cgtggcccag gcggccgttg ttatgaccca gtctccgtct 5160
accctgtctg cttctgttgg tgacaccatc accatcacct gccgtgcttc tcagtctatc 5220
gaaacctggc tggcttggta ccagcagaaa ccgggtaaag ctccgaaact gctgatctac 5280
aaggcttcta ccctgaaaac cggtgttccg tctcgtttct ctggttctgg ttctggtacc 5340
gagttcaccc tgaccatctc tggtctgcag ttcgacgact tcgctaccta ccactgccag 5400
cactacgctg gttactctgc taccttcggt cagggtaccc gtgttgaaat caaaggtggt 5460
tcgtctggat cttcctcctc tggtggcggt ggctcgggcg gtggtggcga agttcagctg 5520
gttgaatctg gtggtggtct ggttaaagct ggtggttctc tgatcctgtc ttgcggtgtt 5580
tctaacttcc gtatctctgc tcacaccatg aactgggttc gtcgtgttcc gggtggtggt 5640
ctggaatggg ttgcttctat ctctacctct tctacctacc gtgactacgc tgacgctgtt 5700
aaaggtcgtt tcaccgtttc tcgtgacgac ctggaagact tcgtttacct gcagatgcac 5760
aaaatgcgtg ttgaagacac cgctatctac tactgcgctc gtaaaggttc tgaccgtctg 5820
tctgacaacg acccgttcga cgcttggggt ccgggtaccg ttgttaccgt ttctccgccg 5880
aaaagctgcg ataaaaccca tacctgcccg ccgtgcccgg gccaggccgg ccagcaccat 5940
caccatcacc atggcgcata cccgtacgac gttccggact acgcttctta ggcggccgca 6000
ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct 6060
gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg 6120
gtcttgaggg gttttttgct gaaaggagga actatatccg gat                   6163


<210> 39
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> 6-amino acid target portion of AC8 heavy chain
      target polypeptide

<400> 39
Val Ala Tyr Met Leu Glu
 1               5      


<210> 40
<211> 122
<212> PRT
<213> Artificial Sequence

<220> 
<223> AC8 heavy chain target polypeptide

<400> 40
Leu Glu Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser Ser Val Lys
 1               5                  10                  15      
Val Ser Cys Lys Ala Ser Gly Gly Ser Phe Ser Ser Tyr Ala Ile Asn
            20                  25                  30          
Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly Gly Leu
        35                  40                  45              
Met Pro Ile Phe Gly Thr Thr Asn Tyr Ala Gln Lys Phe Gln Asp Arg
    50                  55                  60                  
Leu Thr Ile Thr Ala Asp Val Ser Thr Ser Thr Ala Tyr Met Gln Leu
65                  70                  75                  80  
Ser Gly Leu Thr Tyr Glu Asp Thr Ala Met Tyr Tyr Cys Ala Arg Val
                85                  90                  95      
Ala Tyr Met Leu Glu Pro Thr Val Thr Ala Gly Gly Leu Asp Val Trp
            100                 105                 110         
Gly Gln Gly Thr Thr Val Thr Val Ala Ser
        115                 120         


<210> 41
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> AC8 heavy chain CDR3

<400> 41
gttgcctata tgttggaacc taccgtcact gcagggggtt tggacgtc              48

<210> 42
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> 18-nucleotide target portion of AC8 target
      polynucleotide

<400> 42
gttgcctata tgttggaa                                               18

<210> 43
<211> 4
<212> DNA
<213> Artificial Sequence

<220> 
<223> overhang

<400> 43
agct                                                              4

<210> 44
<211> 112
<212> DNA
<213> Artificial Sequence

<220> 
<223> AC8HCDR3org (+)

<400> 44
tatgaagaca cggccatgta ttactgtgcg agagttgcct atatgttgga acctaccgtc 60
actgcagggg gtttggacgt ctggggccaa gggaccacgg tcaccgtgag ct         112

<210> 45
<211> 106
<212> DNA
<213> Artificial Sequence

<220> 
<223> AC8HCDR3org (-)

<400> 45
cacggtgacc gtggtccctt ggccccagac gtccaaaccc cctgcagtga cggtaggttc 60
caacatatag gcaactctcg cacagtaata catggccgtg tcttca                106

<210> 46
<211> 112
<212> DNA
<213> Artificial Sequence

<220> 
<223> AC8HCDR3 (+)

<220> 
<221> misc_feature   
<222> 34-35, 37-38, 40-41, 43-44, 46-47, 49-50
<223> n = a or g or c or t

<220> 
<221> misc_feature   
<222> 36, 39, 42, 45, 48, 51
<223> k = g or t

<400> 46
tatgaagaca cggccatgta ttactgtgcg agannknnkn nknnknnknn kcctaccgtc 60
actgcagggg gtttggacgt ctggggccaa gggaccacgg tcaccgtgag ct         112

<210> 47
<211> 106
<212> DNA
<213> Artificial Sequence

<220> 
<223> AC8HCDR3 (-)

<220> 
<221> misc_feature   
<222> 58, 61, 64, 67, 70, 73
<223> m = a or c

<220> 
<221> misc_feature   
<222> 59-60, 62-63, 65-66, 68-69, 71-72, 74-75
<223> n = a or g or c or t

<400> 47
cacggtgacc gtggtccctt ggccccagac gtccaaaccc cctgcagtga cggtaggmnn 60
mnnmnnmnnm nnmnntctcg cacagtaata catggccgtg tcttca                106

<210> 48
<211> 5371
<212> DNA
<213> Artificial Sequence

<220> 
<223> pET28(a)+ vector

<400> 48
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60
ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120
tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180
cgacggagct cgaattcgga tccgatatca gccatggaac cgcgtggcac cagggtaccc 240
agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatatgt 300
atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca 360
attcccctat agtgagtcgt attaatttcg cgggatcgag atcgatctcg atcctctacg 420
ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg 480
ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg 540
gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg 600
caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa 660
tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc 720
tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa 780
ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc 840
gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg 900
gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg 960
ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg 1020
attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc 1080
ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg 1140
atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat 1200
gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc 1260
catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc 1320
gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat 1380
aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc 1440
atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg 1500
ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg 1560
cgcgttggtg cggacatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat 1620
atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac 1680
cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca 1740
ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 1800
gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 1860
caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt 1920
gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc 1980
acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt 2040
cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt 2100
attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt 2160
cggcgagaag caggccatta tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct 2220
cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc 2280
accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac 2340
aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc 2400
ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc 2460
tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg 2520
catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc 2580
agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag 2640
aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc 2700
ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga 2760
tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg 2820
cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 2880
cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 2940
tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac 3000
tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat atgcggtgtg 3060
aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc 3120
tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 3180
cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 3240
gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 3300
gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 3360
gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 3420
ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 3480
atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 3540
tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 3600
ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 3660
gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 3720
ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 3780
ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 3840
agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 3900
ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aacaataaaa 3960
ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg 4020
tcttgctcta ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg 4080
gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat 4140
gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag 4200
atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc 4260
cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag 4320
gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg 4380
cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt 4440
ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac 4500
gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc 4560
tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag 4620
gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat 4680
cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt 4740
caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat 4800
gagtttttct aagaattaat tcatgagcgg atacatattt gaatgtattt agaaaaataa 4860
acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa 4920
tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 4980
cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 5040
tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 5100
aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 5160
gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 5220
acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 5280
tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 5340
tgcgccgcta cagggcgcgt cccattcgcc a                                5371


<210> 49
<211> 6145
<212> DNA
<213> Artificial Sequence

<220> 
<223> pAC8 sequence

<400> 49
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 60
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 120
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 180
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 240
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 300
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 360
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 420
acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 480
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 540
tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 600
tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 660
actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 720
gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 780
aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 840
agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 900
cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 960
aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1020
tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1080
tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1140
taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1200
ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1260
tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1320
tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1380
cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1440
cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1500
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1560
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1620
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1680
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1740
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1800
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1860
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1920
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1980
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2040
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2100
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2160
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2220
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2280
tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2340
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2400
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2460
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2520
gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2580
gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2640
aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2700
ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2760
acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2820
ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2880
tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2940
tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3000
cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3060
gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3120
ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3180
catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3240
ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3300
gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3360
gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3420
ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3480
atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3540
cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3600
tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3660
ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3720
aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3780
atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3840
cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3900
gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3960
tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4020
agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4080
gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4140
ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4200
catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4260
tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4320
tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4380
gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4440
ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4500
tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4560
catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4620
cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4680
tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4740
ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4800
ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4860
cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4920
gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4980
aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5040
ttttgtttaa ctttaagaag gagatatacc atgaaaaaga cagctatcgc gattgcagtg 5100
gcactggctg gtttcgctac cgtggcccag gcggccgaga tagtcctcac gcagtctcca 5160
ggcaccctgt ctttgtctcc aggggaaaga gccaccctct cctgcagggc cagtcagagt 5220
gttagtagcg cctacttagc ctggtaccag cagaaacctg gccaggctcc caggctcctc 5280
atctatggtg catccagcag ggccactggc atcccagaca ggttcagtgg cagtgggtct 5340
gggacagact tcactctcac catcagcaga ctggaacctg aagattttgc agtgtattac 5400
tgtcagcagt atggtaggtc acccactttc ggcggaggga ccaaggtgga gatcaaaggt 5460
ggttcgtcta gatcttcctc ctctggtggc ggtggctcgg gcggtggtgg ccaggtccag 5520
ctcgtccagt caggggctga ggtgaagaag cctgggtcct cggtgaaggt ctcctgcaag 5580
gcttctggag gttccttcag cagctatgct atcaactggg tgcgacaggc ccctggacaa 5640
gggcttgagt ggatgggagg gctcatgcct atctttggga caacaaacta cgcacagaag 5700
ttccaggaca gactcacgat taccgcggac gtatccacga gtacagccta catgcagctg 5760
agcggcctga catatgaaga cacggccatg tattactgtg cgagagttgc ctatatgttg 5820
gaacctaccg tcactgcagg gggtttggac gtctggggcc aagggaccac ggtcaccgtg 5880
agctcagctt ccaccaaggg cggccaggcc ggccagcacc atcaccatca ccatggcgca 5940
tacccgtacg acgttccgga ctacgcttct taggcggccg cactcgagca ccaccaccac 6000
caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc 6060
gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg 6120
ctgaaaggag gaactatatc cggat                                       6145


<210> 50
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> AC8 (reference sequence) clone

<400> 50
tattactgtg cgagagttgc ctatatgttg gaacctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 51
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> AC8 (reference sequence) clone

<400> 51
Tyr Tyr Cys Ala Arg Val Ala Tyr Met Leu Glu Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 52
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 1 clone

<400> 52
tattactgtg cgagaagtgg ggatgagggg aagcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 53
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 1 clone

<400> 53
Tyr Tyr Cys Ala Arg Ser Gly Asp Glu Gly Lys Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 54
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 3

<400> 54
tattactgtg cgagatttcc gccttttacg cgtcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 55
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 3

<400> 55
Tyr Tyr Cys Ala Arg Phe Pro Pro Phe Thr Arg Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 56
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 4

<400> 56
tattactgtg cgagacgtca gctttttcaa ccgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 57
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 4

<400> 57
Tyr Tyr Cys Ala Arg Arg Gln Leu Phe Gln Pro Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 58
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 5

<400> 58
tattactgtg cgagacatag tccgcagttt tggcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 59
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 5

<400> 59
Tyr Tyr Cys Ala Arg His Ser Pro Gln Phe Trp Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 60
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 6

<400> 60
tattactgtg cgagacctag tccgcagttt tggcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 61
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 6

<400> 61
Tyr Tyr Cys Ala Arg Pro Ser Pro Gln Phe Trp Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 62
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 8

<400> 62
tattactgtg cgagaattgc ggctagtctt ttgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 63
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 8

<400> 63
Tyr Tyr Cys Ala Arg Ile Ala Ala Ser Leu Leu Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 64
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 9

<400> 64
tattactgtg cgagaacttt tacttgggag gctcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 65
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 9

<400> 65
Tyr Tyr Cys Ala Arg Thr Phe Thr Trp Glu Ala Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 66
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 13

<400> 66
tattactgtg cgagagcggt ggtgtaggtt gggcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 67
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 13

<220> 
<221> SITE           
<222> 9
<223> Xaa = stop codon

<400> 67
Tyr Tyr Cys Ala Arg Ala Val Val Xaa Val Gly Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 68
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 15

<400> 68
tattactgtg cgagacggag tgctcttgct catcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 69
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 15

<400> 69
Tyr Tyr Cys Ala Arg Arg Ser Ala Leu Ala His Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 70
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 16

<400> 70
tattactgtg cgagactgcg gccgattccg tttcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 71
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 16

<400> 71
Tyr Tyr Cys Ala Arg Leu Arg Pro Ile Pro Phe Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 72
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 17

<400> 72
tattactgtg cgagaagttg ggtgagtgtg ccgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 73
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 17

<400> 73
Tyr Tyr Cys Ala Arg Ser Trp Val Ser Val Pro Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 74
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 18

<400> 74
tattactgtg cgagatagat ggagttgaat ttgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 75
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 18

<220> 
<221> SITE           
<222> 6
<223> Xaa = stop codon

<400> 75
Tyr Tyr Cys Ala Arg Xaa Met Glu Leu Asn Leu Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 76
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 19

<400> 76
tattactgtg cgagagatct gctttatctt aggcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 77
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 19

<400> 77
Tyr Tyr Cys Ala Arg Asp Leu Leu Tyr Leu Arg Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 78
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 20

<400> 78
tattactgtg cgagagattt ggataatcgt aggcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 79
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 20

<400> 79
Tyr Tyr Cys Ala Arg Asp Leu Asp Asn Arg Arg Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 80
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 22

<400> 80
tattactgtg cgagacggat gatgatgggg gtgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 81
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 22

<400> 81
Tyr Tyr Cys Ala Arg Arg Met Met Met Gly Val Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 82
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 23

<400> 82
tattactgtg cgagagggat gggttagtcg aggcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 83
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 23

<220> 
<221> SITE           
<222> 9
<223> Xaa = stop codon

<400> 83
Tyr Tyr Cys Ala Arg Gly Met Gly Xaa Ser Arg Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 84
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MXD 24

<400> 84
tattactgtg cgagaactcc gacgaatcgg actcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 85
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MXD 24

<400> 85
Tyr Tyr Cys Ala Arg Thr Pro Thr Asn Arg Thr Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 86
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> NdeI restriction site

<400> 86
catatg                                                            6

<210> 87
<211> 5
<212> DNA
<213> Artificial Sequence

<220> 
<223> Sac1 restriction site overhang

<400> 87
gagct                                                             5

<210> 88
<211> 124
<212> DNA
<213> Artificial Sequence

<220> 
<223> AC8HC3 mixed template(+)

<220> 
<221> misc_feature   
<222> 46-47, 49-50, 52-53, 55-56, 58-59, 61-62
<223> n = a or g or c or t

<220> 
<221> misc_feature   
<222> 48, 51, 54, 57, 60, 63
<223> k = g or t

<400> 88
agcggcctga catatgaaga cacggccatg tattactgtg cgagannknn knnknnknnk 60
nnkcctaccg tcactgcagg gggtttggac gtctggggcc aagggaccac ggtcaccgtg 120
agct                                                              124

<210> 89
<211> 124
<212> DNA
<213> Artificial Sequence

<220> 
<223> AC8HC3 native template(+)

<400> 89
agcggcctga catatgaaga cacggccatg tattactgtg cgagagttgc ctatatgttg 60
gaacctaccg tcactgcagg gggtttggac gtctggggcc aagggaccac ggtcaccgtg 120
agct                                                              124

<210> 90
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> AC8H3 fill-in-R

<400> 90
cacggtgacc gtggtccctt gg                                          22

<210> 91
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 1

<400> 91
tattactgtg cgagacgtga ggcggggttt tggcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 92
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 1

<400> 92
Tyr Tyr Cys Ala Arg Arg Glu Ala Gly Phe Trp Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 93
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 2

<400> 93
tattactgtg cgagaaggct gacggtggtg gggcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 94
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 2

<400> 94
Tyr Tyr Cys Ala Arg Arg Leu Thr Val Val Gly Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 95
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 3

<400> 95
tattactgtg cgagaattat gagtacgcat ttgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 96
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 3

<400> 96
Tyr Tyr Cys Ala Arg Ile Met Ser Thr His Leu Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 97
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 4

<400> 97
tattactgtg cgagagagac tgttgcgcag tcgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 98
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 4

<400> 98
Tyr Tyr Cys Ala Arg Glu Thr Val Ala Gln Ser Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 99
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 5

<400> 99
tattactgtg cgagatttgg ttgggttgat tgtcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 100
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 5

<400> 100
Tyr Tyr Cys Ala Arg Phe Gly Trp Val Asp Cys Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 101
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 6

<400> 101
tattactgtg cgagatttgt gcagatgtag tggcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 102
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 6

<220> 
<221> SITE           
<222> 10
<223> Xaa = stop codon

<400> 102
Tyr Tyr Cys Ala Arg Phe Val Gln Met Xaa Trp Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 103
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 8

<400> 103
tattactgtg cgagacgtaa tcttctggtt aagcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 104
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 8

<400> 104
Tyr Tyr Cys Ala Arg Arg Asn Leu Leu Val Lys Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 105
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 11

<400> 105
tattactgtg cgagaagttc tctgtggagg gttcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 106
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 11

<400> 106
Tyr Tyr Cys Ala Arg Ser Ser Leu Trp Arg Val Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 107
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 12

<400> 107
tattactgtg cgagactggc ggatatgttt aagcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 108
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 12

<400> 108
Tyr Tyr Cys Ala Arg Leu Ala Asp Met Phe Lys Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 109
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 13

<400> 109
tattactgtg cgagatttcg ttgttatgct actcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 110
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 13

<400> 110
Tyr Tyr Cys Ala Arg Phe Arg Cys Tyr Ala Thr Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 111
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 15

<400> 111
tattactgtg cgagagggac ggggacgcgg tcgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 112
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 15

<400> 112
Tyr Tyr Cys Ala Arg Gly Thr Gly Thr Arg Ser Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 113
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 16

<400> 113
tattactgtg cgagacagct gagggagagt gttcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 114
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 16

<400> 114
Tyr Tyr Cys Ala Arg Gln Leu Arg Glu Ser Val Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 115
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 17

<400> 115
tattactgtg cgagagctaa gcggggttgg actcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 116
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 17

<400> 116
Tyr Tyr Cys Ala Arg Ala Lys Arg Gly Trp Thr Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 117
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 20

<400> 117
tattactgtg cgagactgca tgggcggcct atgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 118
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 20

<400> 118
Tyr Tyr Cys Ala Arg Leu His Gly Arg Pro Met Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 119
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 21

<400> 119
tattactgtg cgagaagggt tgagagtagg ctgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 120
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 21

<400> 120
Tyr Tyr Cys Ala Arg Arg Val Glu Ser Arg Leu Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 121
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 22

<400> 121
tattactgtg cgagaacggg tggtgagggt tcgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 122
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 22

<400> 122
Tyr Tyr Cys Ala Arg Thr Gly Gly Glu Gly Ser Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 123
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 23

<400> 123
tattactgtg cgagactgtt taagattggg gtgcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 124
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 23

<400> 124
Tyr Tyr Cys Ala Arg Leu Phe Lys Ile Gly Val Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 125
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> MFILL_ 24

<400> 125
tattactgtg cgagacggga taggaagcgt tatcctaccg tcactgcagg gggtttggac 60
gtctggggcc aa                                                     72

<210> 126
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> MFILL_ 24

<400> 126
Tyr Tyr Cys Ala Arg Arg Asp Arg Lys Arg Tyr Pro Thr Val Thr Ala
 1               5                  10                  15      
Gly Gly Leu Asp Val Trp Gly Gln
            20                  


<210> 127
<211> 228
<212> PRT
<213> Artificial Sequence

<220> 
<223> 3-ALA 2G12 domain exchanged Fab heavy chain
      (VH-CH1)

<400> 127
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Ala Gly Gly
 1               5                  10                  15      
Ser Leu Ile Leu Ser Cys Gly Val Ser Asn Phe Arg Ile Ser Ala His
            20                  25                  30          
Thr Met Asn Trp Val Arg Arg Val Pro Gly Gly Gly Leu Glu Trp Val
        35                  40                  45              
Ala Ser Ile Ser Thr Ser Ser Thr Tyr Arg Asp Tyr Ala Asp Ala Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Val Ser Arg Asp Asp Leu Glu Asp Phe Val Tyr
65                  70                  75                  80  
Leu Gln Met His Lys Met Arg Val Glu Asp Thr Ala Ile Tyr Tyr Cys
                85                  90                  95      
Ala Arg Lys Gly Ser Asp Arg Ala Ala Asp Ala Asp Pro Phe Asp Ala
            100                 105                 110         
Trp Gly Pro Gly Thr Val Val Thr Val Ser Pro Ala Ser Thr Lys Gly
        115                 120                 125             
Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly
    130                 135                 140                 
Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val
145                 150                 155                 160 
Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe
                165                 170                 175     
Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val
            180                 185                 190         
Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val
        195                 200                 205             
Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys
    210                 215                 220                 
Ser Cys Leu Arg
225             


<210> 128
<211> 228
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2G12 domain exchanged Fab heavy chain (VH-CH1)

<400> 128
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Ala Gly Gly
 1               5                  10                  15      
Ser Leu Ile Leu Ser Cys Gly Val Ser Asn Phe Arg Ile Ser Ala His
            20                  25                  30          
Thr Met Asn Trp Val Arg Arg Val Pro Gly Gly Gly Leu Glu Trp Val
        35                  40                  45              
Ala Ser Ile Ser Thr Ser Ser Thr Tyr Arg Asp Tyr Ala Asp Ala Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Val Ser Arg Asp Asp Leu Glu Asp Phe Val Tyr
65                  70                  75                  80  
Leu Gln Met His Lys Met Arg Val Glu Asp Thr Ala Ile Tyr Tyr Cys
                85                  90                  95      
Ala Arg Lys Gly Ser Asp Arg Leu Ser Asp Asn Asp Pro Phe Asp Ala
            100                 105                 110         
Trp Gly Pro Gly Thr Val Val Thr Val Ser Pro Ala Ser Thr Lys Gly
        115                 120                 125             
Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly
    130                 135                 140                 
Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val
145                 150                 155                 160 
Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe
                165                 170                 175     
Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val
            180                 185                 190         
Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val
        195                 200                 205             
Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys
    210                 215                 220                 
Ser Cys Leu Arg
225             


<210> 129
<211> 215
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2G12 and 3-ALA light chain (domain exchanged Fab)

<400> 129
Ala Gly Val Val Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val
 1               5                  10                  15      
Gly Asp Thr Ile Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Glu Thr
            20                  25                  30          
Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
        35                  40                  45              
Ile Tyr Lys Ala Ser Thr Leu Lys Thr Gly Val Pro Ser Arg Phe Ser
    50                  55                  60                  
Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Gly Leu Gln
65                  70                  75                  80  
Phe Asp Asp Phe Ala Thr Tyr His Cys Gln His Tyr Ala Gly Tyr Ser
                85                  90                  95      
Ala Thr Phe Gly Gln Gly Thr Arg Val Glu Ile Lys Arg Thr Val Ala
            100                 105                 110         
Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser
        115                 120                 125             
Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu
    130                 135                 140                 
Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser
145                 150                 155                 160 
Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu
                165                 170                 175     
Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val
            180                 185                 190         
Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys
        195                 200                 205             
Ser Phe Asn Arg Gly Glu Cys
    210                 215 


<210> 130
<211> 684
<212> DNA
<213> Artificial Sequence

<220> 
<223> NT encoding 3-ALA 2G12 Fab  heavy chain

<400> 130
gaagttcagc tggttgaatc tggtggtggt ctggttaaag ctggtggttc tctgatcctg 60
tcttgcggtg tttctaactt ccgtatctct gctcacacca tgaactgggt tcgtcgtgtt 120
ccgggtggtg gtctggaatg ggttgcttct atctctacct cttctaccta ccgtgactac 180
gctgacgctg ttaaaggtcg tttcaccgtt tctcgtgacg acctggaaga cttcgtttac 240
ctgcagatgc ataaaatgcg tgttgaagac accgctatct actactgcgc tcgtaaaggt 300
tctgaccgtg cggcggacgc ggacccgttc gacgcttggg gtccgggtac cgttgttacc 360
gtttctccgg cgtcgaccaa aggtccgtct gttttcccgc tggctccgtc ttctaaatct 420
acctctggtg gtaccgctgc tctgggttgc ctggttaaag actacttccc ggaaccggtt 480
accgtttctt ggaactctgg tgctctgacc tctggtgttc acaccttccc ggctgttctg 540
cagtcttctg gtctgtactc tctgtcttct gttgttaccg ttccgtcttc ttctctgggt 600
acccagacct acatctgcaa cgttaaccac aaaccgtcta acaccaaagt tgacaagaaa 660
gttgaaccga aatcttgcct gcga                                        684

<210> 131
<211> 645
<212> DNA
<213> Artificial Sequence

<220> 
<223> NT encoding 3-ALA 2G12 and 2G12 Fab light chain

<400> 131
gccggtgttg ttatgaccca gtctccgtct accctgtctg cttctgttgg tgacaccatc 60
accatcacct gccgtgcttc tcagtctatc gaaacctggc tggcttggta ccagcagaaa 120
ccgggtaaag ctccgaaact gctgatctac aaggcttcta ccctgaaaac cggtgttccg 180
tctcgtttct ctggttctgg ttctggtacc gagttcaccc tgaccatctc tggtctgcag 240
ttcgacgact tcgctaccta ccactgccag cactacgctg gttactctgc taccttcggt 300
cagggtaccc gtgttgaaat caaacgtacc gttgctgctc cgtctgtttt catcttcccg 360
ccgtctgacg aacagctgaa atctggtacc gcttctgttg tttgcctgct gaacaacttc 420
tacccgcgtg aagctaaagt tcagtggaaa gttgacaacg ctctgcagtc tggtaactct 480
caggaatctg ttaccgaaca ggactctaaa gactctacct actctctgtc ttctaccctg 540
accctgtcta aagctgacta cgaaaagcac aaagtttacg cttgcgaagt tacccaccag 600
ggtctgtctt ctccggttac caaatctttc aaccgtggtg aatgc                 645

<210> 132
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide pool A / primer A

<400> 132
gcccaggcgg ccgcagaagt tcag                                        24

<210> 133
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide pool  B

<220> 
<221> misc_feature   
<222> 23
<223> m = a or c

<220> 
<221> misc_feature   
<222> 24-25, 27-28
<223> n = a or g or c or t

<400> 133
gaacacgacg aacccagttc atmnnannag cagagatacg gaagttag              48

<210> 134
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide pool  C

<220> 
<221> misc_feature   
<222> 21-22, 24-25
<223> n = a or g or c or t

<220> 
<221> misc_feature   
<222> 26
<223> k = g or t

<400> 134
ctaacttccg tatctctgct nntnnkatga actgggttcg tcgtgttc              48

<210> 135
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> oligonucleotide pool  D

<220> 
<221> misc_feature   
<222> 23, 26, 32, 47
<223> m = a or c

<220> 
<221> misc_feature   
<222> 24-25, 27-28, 33-34, 36-37, 48-49
<223> n = a or g or c or t

<400> 135
ccggacccca agcgtcgaac ggmnnmnngt cmnnannacg gtcagamnnt ttacgagcgc 60
agtagtagat ag                                                     72

<210> 136
<211> 398
<212> DNA
<213> Artificial Sequence

<220> 
<223> 3-ALA 2G12 OFIM reference sequence polynucleotide

<400> 136
gcccaggcgg ccgcagaagt tcagctggtt gaatctggtg gtggtctggt taaagctggt 60
ggttctctga tcctgtcttg cggtgtttct aacttccgta tctctgctca caccatgaac 120
tgggttcgtc gtgttccggg tggtggtctg gaatgggttg cttctatctc tacctcttct 180
acctaccgtg actacgctga cgctgttaaa ggtcgtttca ccgtttctcg tgacgacctg 240
gaagacttcg tttacctgca gatgcataaa atgcgtgttg aagacaccgc tatctactac 300
tgcgctcgta aaggttctga ccgtgcggcg gacgcggacc cgttcgacgc ttggggtccg 360
ggtaccgttg ttaccgtttc tccggcgtcg accaaagg                         398

<210> 137
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<223> H1

<400> 137
ggccgcagaa gttcagctgg ttgaatctgg tggtggtctg gttaaagctg gtggttctct 60
gatcctgtct tgcggt                                                 76

<210> 138
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<223> H2

<400> 138
gaagttagaa acaccgcaag acaggatcag agaaccacca gctttaacca gaccaccacc 60
agattcaacc agctgaactt ctgc                                        84

<210> 139
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> H3

<220> 
<221> misc_feature   
<222> 25-26, 28-29
<223> n = a or g or c or t

<220> 
<221> misc_feature   
<222> 30
<223> k = g or t

<400> 139
gtttctaact tccgtatctc tgctnntnnk atgaactggg                       40

<210> 140
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reference sequence used to design H3

<400> 140
gtttctaact tccgtatctc tgctcacacc atgaactggg                       40

<210> 141
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> H4

<220> 
<221> misc_feature   
<222> 23
<223> m = a or c

<220> 
<221> misc_feature   
<222> 24-25, 27-28
<223> n = a or g or c or t

<400> 141
gaacacgacg aacccagttc atmnnannag cagagatacg                       40

<210> 142
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reference Sequence used to design H4

<400> 142
gaacacgacg aacccagttc atggtgtgag cagagatacg                       40

<210> 143
<211> 82
<212> DNA
<213> Artificial Sequence

<220> 
<223> H5

<400> 143
ttcgtcgtgt tccgggtggt ggtctggaat gggttgcttc tatctctacc tcttctacct 60
accgtgacta cgctgacgct gt                                          82

<210> 144
<211> 82
<212> DNA
<213> Artificial Sequence

<220> 
<223> H6

<400> 144
aaacgacctt taacagcgtc agcgtagtca cggtaggtag aagaggtaga gatagaagca 60
acccattcca gaccaccacc cg                                          82

<210> 145
<211> 82
<212> DNA
<213> Artificial Sequence

<220> 
<223> H7

<400> 145
taaaggtcgt ttcaccgttt ctcgtgacga cctggaagac ttcgtttacc tgcagatgca 60
taaaatgcgt gttgaagaca cc                                          82

<210> 146
<211> 82
<212> DNA
<213> Artificial Sequence

<220> 
<223> H8

<400> 146
gtagtagata gcggtgtctt caacacgcat tttatgcatc tgcaggtaaa cgaagtcttc 60
caggtcgtca cgagaaacgg tg                                          82

<210> 147
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> H9

<220> 
<221> misc_feature   
<222> 25-26, 37-38, 40-41, 46-47, 49-50
<223> n = a or g or c or t

<220> 
<221> misc_feature   
<222> 27, 42, 48, 51
<223> k = g or t

<400> 147
gctatctact actgcgctcg taaannktct gaccgtnntn nkgacnnknn kccgttcgac 60
gcttggggt                                                         69

<210> 148
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reference Sequence Used to Design H9

<400> 148
gctatctact actgcgctcg taaaggttct gaccgtctgt ctgacaacga cccgttcgac 60
gcttggggt                                                         69

<210> 149
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> H10

<220> 
<221> misc_feature   
<222> 31, 34, 40, 54
<223> m = a or c

<220> 
<221> misc_feature   
<222> 32-33, 35-36, 41-42, 44-45, 55-56
<223> n = a or g or c or t

<400> 149
aacggtaccc ggaccccaag cgtcgaacgg mnnmnngtcm nnannacggt cagamnnttt 60
acgagcgca                                                         69

<210> 150
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reference Sequence Used to Design H10

<400> 150
aacggtaccc ggaccccaag cgtcgaacgg gtcgttgtca gacagacggt cagaaccttt 60
acgagcgca                                                         69

<210> 151
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> H11

<400> 151
ccgggtaccg ttgttaccgt ttctccggcg                                  30

<210> 152
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> H12

<400> 152
tcgacgccgg agaaacggta ac                                          22

<210> 153
<211> 107
<212> DNA
<213> Artificial Sequence

<220> 
<223> F1b

<400> 153
gcccaggcgg ccgcagaagt tcagctggtt gaatctggtg gtggtctggt taaagctggt 60
ggttctctga tcctgtcttg tggtgtgagc aacttccgca tcagcgc               107

<210> 154
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> F2b

<400> 154
tgatgcggaa gttgctcaca ccac                                        24

<210> 155
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> F3b

<220> 
<221> misc_feature   
<222> 13-14, 16-17
<223> n = a or g or c or t

<220> 
<221> misc_feature   
<222> 18
<223> k = g or t

<400> 155
cgtatcagcg ctnntnnkat gaactgggtg cgccgtgtgc                       40

<210> 156
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reference Sequence used to design F3b

<400> 156
cgtatcagcg ctcacaccat gaactgggtg cgccgtgtgc                       40

<210> 157
<211> 124
<212> DNA
<213> Artificial Sequence

<220> 
<223> F4b

<400> 157
ggtcgtcccg ggaaacggtg aaacgacctt taacagcgtc agcgtagtca cggtaggtag 60
aagaggtaga gatagaagca acccattcca gaccaccacc cggcacacgg cgcacccagt 120
tcat                                                              124

<210> 158
<211> 92
<212> DNA
<213> Artificial Sequence

<220> 
<223> F5b

<400> 158
ccgtttctcg tgacgacctg gaagacttcg tttacctgca gatgcataaa atgcgtgttg 60
aagacaccgc tatctactac tgcgcgcgca ac                               92

<210> 159
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> F6b

<220> 
<221> misc_feature   
<222> 15
<223> m = a or c

<220> 
<221> misc_feature   
<222> 16-17
<223> n = a or g or c or t

<400> 159
gacagacggt cagamnngtt gcgcgcgcag tagtagatag                       40

<210> 160
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reference Sequence used to design F6b

<400> 160
gacagacggt cagaaccgtt gcgcgcgcag tagtagatag                       40

<210> 161
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> F7b

<220> 
<221> misc_feature   
<222> 14-15, 17-18, 23-24, 26-27
<223> n = a or g or c or t

<220> 
<221> misc_feature   
<222> 19, 25, 28
<223> k = g or t

<400> 161
aggtagcgat cgtnntnnkg acnnknnkcc gtttgacgcg tggggtccgg            50

<210> 162
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reference Sequence used to design F7b

<400> 162
aggtagcgat cgtctgtctg acaacgaccc gtttgacgcg tggggtccgg            50

<210> 163
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> F8b

<400> 163
cctttggtcg acgccggaga aacggtaaca acggtacccg gaccccacgc gtcaaacg   58

<210> 164
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> H0m Primer

<400> 164
cctttggtcg acgccggaga aacggtaaca acggtacccg gaccccacgc gtcaaacg   58

<210> 165
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> H0 Primer

<400> 165
gaagttagaa acaccgcaag acaggatcag agaaccacca gctttaac              48

<210> 166
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> H1m Primer

<400> 166
cagaccacca ccagattcaa ccagctgaac ttctgcggcc gcgttactga gcgatggcac 60
agcggc                                                            66

<210> 167
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> H11m Primer

<400> 167
gccgctgtgc catcgctcag taacgc                                      26

<210> 168
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> H12m / CALX24H5-R Primer

<400> 168
gccgctgtgc catcgctcag taacgtcgac gccggagaaa cggtaac               47

<210> 169
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> H1s-R Primer

<400> 169
agacaggatc agagaaccac cag                                         23

<210> 170
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> H1L-F Primer

<400> 170
gcggccgcag aagttcagct g                                           21

<210> 171
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> H1L-R Primer

<400> 171
agcagagata cggaagttag aaac                                        24

<210> 172
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> H2-F Primer

<400> 172
tgcggtgttt ctaacttccg tatctctgct                                  30

<210> 173
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> H2-R Primer

<400> 173
accacccgga acacgacgaa cccagttcat                                  30

<210> 174
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> H3L-F Primer

<400> 174
atgaactggg ttcgtcgtgt tccg                                        24

<210> 175
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> H3L-R Primer

<400> 175
tttacgagcg cagtagtaga tagc                                        24

<210> 176
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> H3S-F Primer

<400> 176
ggtctggaat gggttgcttc tatc                                        24

<210> 177
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> H3S-R Primer

<400> 177
ttcaacacgc attttatgca tctg                                        24

<210> 178
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> H4-F Primer

<400> 178
gacaccgcta tctactactg cgctcgtaaa                                  30

<210> 179
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> H4-R Primer

<400> 179
aacggtaccc ggaccccaag cgtcgaacgg                                  30

<210> 180
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> H5-F Primer

<400> 180
ccgttcgacg cttggggtcc g                                           21

<210> 181
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> CALX24H5-F Primer

<400> 181
gttaccgttt ctccggcgtc gacgttactg agcgatggca cagcggc               47

<210> 182
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> R1 Primer

<400> 182
ggcggcgctc ttcagttaga aacaccgcaa gacaggatc                        39

<210> 183
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> F2 Primer

<400> 183
ggcggcgctc ttctcgtgtt ccgggtggtg gtctg                            35

<210> 184
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> R2 Primer

<400> 184
ggcggcgctc ttcagtagat agcggtgtct tcaacac                          37

<210> 185
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> F3 Primer

<400> 185
ggcggcgctc ttcgggtccg ggtaccgttg ttac                             34

<210> 186
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> R3 Primer

<400> 186
gccgctgtgc catcgctcag taacgtcgac gccggagaaa cggt                  44

<210> 187
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> H1F Primer

<220> 
<221> misc_feature   
<222> 19-20, 22-23
<223> n = a or g or c or t

<220> 
<221> misc_feature   
<222> 24
<223> k = g or t

<400> 187
aacttccgta tctctgctnn tnnkatgaac tgggttcgt                        39

<210> 188
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> H1R Primer

<220> 
<221> misc_feature   
<222> 19
<223> m = a or c

<220> 
<221> misc_feature   
<222> 20-21, 23-24
<223> n = a or g or c or t

<400> 188
acgacgaacc cagttcatmn nannagcaga gatacggaa                        39

<210> 189
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> H3F Primer

<220> 
<221> misc_feature   
<222> 19-20, 32-32, 34-35, 40-41, 43-44
<223> n = a or g or c or t

<220> 
<221> misc_feature   
<222> 21, 36, 42, 45
<223> k = g or t

<400> 189
tactactgcg ctcgtaaann ktctgaccgt nntnnkgacn nknnkccgtt cgacgcttgg 60


<210> 190
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> H3R Primer

<220> 
<221> misc_feature   
<222> 19, 22, 28, 43
<223> m = a or c

<220> 
<221> misc_feature   
<222> 20-21, 23-24, 29-30, 32-33, 44-45
<223> n = a or g or c or t

<400> 190
accccaagcg tcgaacggmn nmnngtcmnn annacggtca gamnntttac gagcgcagta 60


<210> 191
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_0

<400> 191
agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 60
cagctggcac                                                        70

<210> 192
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_1

<400> 192
gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 60
actcattagg caccccaggc tttac                                       85

<210> 193
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_2

<400> 193
actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa ttgaattaag 60
gaggatataa ttatgaaata cctgc                                       85

<210> 194
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_3

<400> 194
tgccgaccgc agccgctggt ctgctgctgc tcgcggccca gccggccatg gccgccggtg 60
cctaactctg gctggtttcg ctacc                                       85

<210> 195
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_4

<400> 195
gtaaccggtt taattaataa ggaggatata attatgaaaa agacagctat cgcgattgca 60
gtggcactgg ctggtttcgc taccg                                       85

<210> 196
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_5

<400> 196
tagcccaggc ggccgcacgc gtctggttga atctggtggg gtctggaatt ctgcgatcgc 60
ggccaggccg gccgcaccat cacca                                       85

<210> 197
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_6

<400> 197
tcaccatggc gcatacccgt acgacgttcc ggactacgct tcta                  44

<210> 198
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_7

<400> 198
ctagtagaag cgtagtccgg aacgtcgtac gggtatgcgc catggtgatg gtgatggtgc 60
ggccggcctg                                                        70

<210> 199
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_8

<400> 199
gccgcgatcg cagaattcca gaccccacca gattcaacca gacgcgtgcg gccgcctggg 60
ctacggtagc gaaaccagcc agtgc                                       85

<210> 200
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_9

<400> 200
cactgcaatc gcgatagctg tctttttcat aattatatcc tccttattaa ttaaaccggt 60
tacggtagcg aaaccagcca gagtt                                       85

<210> 201
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_10

<400> 201
aggcaccggc ggccatggcc ggctgggccg cgagcagcag cagaccagcg gctgcggtcg 60
gcagcaggta tttcataatt atatc                                       85

<210> 202
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_11

<400> 202
ctccttaatt caattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 60
agtgtaaagc ctggggtgcc taatg                                       85

<210> 203
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_12

<400> 203
agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 60
gtcgtgccag ctgcattaat gaatc                                       85

<210> 204
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCAL_13

<400> 204
ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttcc                 45

<210> 205
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> SpeIG3-F Primer

<400> 205
ggtggtggtt ctggtactag ttaggagggt ggtg                             34

<210> 206
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> PvuINheIG3-R Primer

<400> 206
gggaagggcg atcgttagct agcttaagac tccttattac gcagtatgtt ag         52

<210> 207
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> SpeG3A-F Primer

<400> 207
ggtggtggtt ctggtactag ttagaagggt ggtg                             34

<210> 208
<211> 923
<212> DNA
<213> Artificial Sequence

<220> 
<223> partial pAC8 sequence in vector

<400> 208
atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtggcccag 60
gcggccgaga tagtcctcac gcagtctcca ggcaccctgt ctttgtctcc aggggaaaga 120
gccaccctct cctgcagggc cagtcagagt gttagtagcg cctacttagc ctggtaccag 180
cagaaacctg gccaggctcc caggctcctc atctatggtg catccagcag ggccactggc 240
atcccagaca ggttcagtgg cagtgggtct gggacagact tcactctcac catcagcaga 300
ctggaacctg aagattttgc agtgtattac tgtcagcagt atggtaggtc acccactttc 360
ggcggaggga ccaaggtgga gatcaaaggt ggttcgtcta gatcttcctc ctctggtggc 420
ggtggctcgg gcggtggtgg ccaggtccag ctcgtccagt caggggctga ggtgaagaag 480
cctgggtcct cggtgaaggt ctcctgcaag gcttctggag gttccttcag cagctatgct 540
atcaactggg tgcgacaggc ccctggacaa gggcttgagt ggatgggagg gctcatgcct 600
atctttggga caacaaacta cgcacagaag ttccaggaca gactcacgat taccgcggac 660
gtatccacga gtacagccta catgcagctg agcggcctga catatgaaga cacggccatg 720
tattactgtg cgagagttgc ctatatgttg gaacctaccg tcactgcagg gggtttggac 780
gtctggggcc aagggaccac ggtcaccgtg agctcagctt ccaccaaggg cggccaggcc 840
ggccagcacc atcaccatca ccatggcgca tacccgtacg acgttccgga ctacgcttct 900
taggagggtg gtggctctga ggg                                         923

<210> 209
<211> 108
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2G12 VL domain (also 3-ALA VL) 2

<400> 209
Ala Gly Val Val Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val
 1               5                  10                  15      
Gly Asp Thr Ile Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Glu Thr
            20                  25                  30          
Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
        35                  40                  45              
Ile Tyr Lys Ala Ser Thr Leu Lys Thr Gly Val Pro Ser Arg Phe Ser
    50                  55                  60                  
Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Gly Leu Gln
65                  70                  75                  80  
Phe Asp Asp Phe Ala Thr Tyr His Cys Gln His Tyr Ala Gly Tyr Ser
                85                  90                  95      
Ala Thr Phe Gly Gln Gly Thr Arg Val Glu Ile Lys
            100                 105             


<210> 210
<211> 684
<212> DNA
<213> Artificial Sequence

<220> 
<223> NT encoding 2G12 Fab  heavy chain

<400> 210
gaagttcagc tggttgaatc tggtggtggt ctggttaaag ctggtggttc tctgatcctg 60
tcttgcggtg tttctaactt ccgtatctct gctcacacca tgaactgggt tcgtcgtgtt 120
ccgggtggtg gtctggaatg ggttgcttct atctctacct cttctaccta ccgtgactac 180
gctgacgctg ttaaaggtcg tttcaccgtt tctcgtgacg acctggaaga cttcgtttac 240
ctgcagatgc ataaaatgcg tgttgaagac accgctatct actactgcgc tcgtaaaggt 300
tctgaccgtc tgtctgacaa cgacccgttc gacgcttggg gtccgggtac cgttgttacc 360
gtttctccgg cgtcgaccaa aggtccgtct gttttcccgc tggctccgtc ttctaaatct 420
acctctggtg gtaccgctgc tctgggttgc ctggttaaag actacttccc ggaaccggtt 480
accgtttctt ggaactctgg tgctctgacc tctggtgttc acaccttccc ggctgttctg 540
cagtcttctg gtctgtactc tctgtcttct gttgttaccg ttccgtcttc ttctctgggt 600
acccagacct acatctgcaa cgttaaccac aaaccgtcta acaccaaagt tgacaagaaa 660
gttgaaccga aatcttgcct gcga                                        684

<210> 211
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> nt sequence encoding HA tag

<400> 211
tacccgtacg acgttccgga ctacgct                                     27

<210> 212
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> AA sequence - HA tag

<400> 212
Tyr Pro Tyr Asp Val Pro Asp Tyr Ala
 1               5                  


<210> 213
<211> 6840
<212> DNA
<213> Artificial Sequence

<220> 
<223> 2G12 heavy and light chain in pET Duet vector
      (Fab)

<400> 213
ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag 60
gagatatacc atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac 120
cgtggcccag gcggccgttg ttatgaccca gtctccgtct accctgtctg cttctgttgg 180
tgacaccatc accatcacct gccgtgcttc tcagtctatc gaaacctggc tggcttggta 240
ccagcagaaa ccgggtaaag ctccgaaact gctgatctac aaggcttcta ccctgaaaac 300
cggtgttccg tctcgtttct ctggttctgg ttctggtacc gagttcaccc tgaccatctc 360
tggtctgcag ttcgacgact tcgctaccta ccactgccag cactacgctg gttactctgc 420
taccttcggt cagggtaccc gtgttgaaat caaacgtacc gttgctgctc cgtctgtttt 480
catcttcccg ccgtctgacg aacagctgaa atctggtacc gcttctgttg tttgcctgct 540
gaacaacttc tacccgcgtg aagctaaagt tcagtggaaa gttgacaacg ctctgcagtc 600
tggtaactct caggaatctg ttaccgaaca ggactctaaa gactctacct actctctgtc 660
ttctaccctg accctgtcta aagctgacta cgaaaagcac aaagtttacg cttgcgaagt 720
tacccaccag ggtctgtctt ctccggttac caaatctttc aaccgtggtg aatgctaggg 780
ccaggccggc cgcggccgca taatgcttaa gtcgaacaga aagtaatcgt attgtacacg 840
gccgcataat cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc 900
ccatcttagt atattagtta agtataagaa ggagatatac atatgaaata cctattgcct 960
acggcagccg ctggattgtt attactcgct gcccaaccag ccatggccga agttcagctg 1020
gttgaatctg gtggtggtct ggttaaagct ggtggttctc tgatcctgtc ttgcggtgtt 1080
tctaacttcc gtatctctgc tcacaccatg aactgggttc gtcgtgttcc gggtggtggt 1140
ctggaatggg ttgcttctat ctctacctct tctacctacc gtgactacgc tgacgctgtt 1200
aaaggtcgtt tcaccgtttc tcgtgacgac ctggaagact tcgtttacct gcagatgcat 1260
aaaatgcgtg ttgaagacac cgctatctac tactgcgctc gtaaaggttc tgaccgtctg 1320
tctgacaacg acccgttcga cgcttggggt ccgggtaccg ttgttaccgt ttctccggcg 1380
tcgaccaaag gtccgtctgt tttcccgctg gctccgtctt ctaaatctac ctctggtggt 1440
accgctgctc tgggttgcct ggttaaagac tacttcccgg aaccggttac cgtttcttgg 1500
aactctggtg ctctgacctc tggtgttcac accttcccgg ctgttctgca gtcttctggt 1560
ctgtactctc tgtcttctgt tgttaccgtt ccgtcttctt ctctgggtac ccagacctac 1620
atctgcaacg ttaaccacaa accgtctaac accaaagttg acaagaaagt tgaaccgaaa 1680
agctgcgata aaacccatac ctgcccgccg tgcccgcacc atcaccatca ccatggcgca 1740
tacccgtacg acgttccgga ctacgcttct tagctcgagt ctggtaaaga aaccgctgct 1800
gcgaaatttg aacgccagca catggactcg tctactagcg cagcttaatt aacctaggct 1860
gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg 1920
ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg 1980
gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 2040
ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 2100
cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 2160
tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 2220
cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 2280
ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 2340
tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 2400
aaatattaac gtttacaatt tctggcggca cgatggcatg agattatcaa aaaggatctt 2460
cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 2520
aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 2580
atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 2640
cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 2700
tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 2760
atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 2820
taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 2880
tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 2940
gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 3000
cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 3060
cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 3120
gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 3180
aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 3240
accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 3300
ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 3360
gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatcatgatt 3420
gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 3480
ataaacaaat aggtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3540
gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3600
tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 3660
ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 3720
ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 3780
gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 3840
ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 3900
tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 3960
ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 4020
agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 4080
agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 4140
gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 4200
tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 4260
accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 4320
gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt 4380
atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 4440
gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 4500
aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 4560
tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 4620
gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg 4680
ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa 4740
gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg 4800
atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt 4860
tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg 4920
gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga 4980
tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt 5040
gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca 5100
tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat 5160
cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga 5220
caggagcacg atcatgctag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa 5280
ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat 5340
tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 5400
aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt 5460
tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca 5520
gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg 5580
gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac 5640
caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg 5700
caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac 5760
cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga 5820
gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta 5880
acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt 5940
cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg 6000
ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt 6060
taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt 6120
cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag 6180
atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc 6240
caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca 6300
gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt 6360
tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg 6420
ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac 6480
cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac 6540
tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg 6600
aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata 6660
cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg 6720
atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg 6780
cgtccggcgt agaggatcga gatcgatctc gatcccgcga aattaatacg actcactata 6840


<210> 214
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCALVL-F Primer

<400> 214
ccatggccgc cggtgttgtt atgacccagt ctccgtc                          37

<210> 215
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCALCK-R Primer

<400> 215
ctccttatta attaattagc attcaccacg gttgaaag                         38

<210> 216
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> pCALCH-R Primer

<400> 216
ctggccgcga tcgcaggcaa gatttcggtt caactttctt g                     41

<210> 217
<211> 4765
<212> DNA
<213> Artificial Sequence

<220> 
<223> 2G12 pCAL A1 vector

<400> 217
gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 60
caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 120
ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 180
gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 240
tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 300
ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 360
tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 420
atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 480
gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 540
caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 600
ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 660
ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 720
ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 780
ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 840
gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 900
ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 960
taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1020
agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1080
atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1140
aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200
caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1260
ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1320
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1380
tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1440
gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1500
ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1560
gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1620
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1680
ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1740
tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1800
ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1860
agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1920
aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1980
gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 2040
tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2100
tgtgtggaat tgtgagcgga taacaattga attaaggagg atataattat gaaatacctg 2160
ctgccgaccg cagccgctgg tctgctgctg ctcgcggccc agccggccat ggccgccggt 2220
gttgttatga cccagtctcc gtctaccctg tctgcttctg ttggtgacac catcaccatc 2280
acctgccgtg cttctcagtc tatcgaaacc tggctggctt ggtaccagca gaaaccgggt 2340
aaagctccga aactgctgat ctacaaggct tctaccctga aaaccggtgt tccgtctcgt 2400
ttctctggtt ctggttctgg taccgagttc accctgacca tctctggtct gcagttcgac 2460
gacttcgcta cctaccactg ccagcactac gctggttact ctgctacctt cggtcagggt 2520
acccgtgttg aaatcaaacg taccgttgct gctccgtctg ttttcatctt cccgccgtct 2580
gacgaacagc tgaaatctgg taccgcttct gttgtttgcc tgctgaacaa cttctacccg 2640
cgtgaagcta aagttcagtg gaaagttgac aacgctctgc agtctggtaa ctctcaggaa 2700
tctgttaccg aacaggactc taaagactct acctactctc tgtcttctac cctgaccctg 2760
tctaaagctg actacgaaaa gcacaaagtt tacgcttgcg aagttaccca ccagggtctg 2820
tcttctccgg ttaccaaatc tttcaaccgt ggtgaatgct aattaattaa taaggaggat 2880
ataattatga aaaagacagc tatcgcgatt gcagtggcac tggctggttt cgctaccgta 2940
gcccaggcgg ccgcagaagt tcagctggtt gaatctggtg gtggtctggt taaagctggt 3000
ggttctctga tcctgtcttg cggtgtttct aacttccgta tctctgctca caccatgaac 3060
tgggttcgtc gtgttccggg tggtggtctg gaatgggttg cttctatctc tacctcttct 3120
acctaccgtg actacgctga cgctgttaaa ggtcgtttca ccgtttctcg tgacgacctg 3180
gaagacttcg tttacctgca gatgcataaa atgcgtgttg aagacaccgc tatctactac 3240
tgcgctcgta aaggttctga ccgtctgtct gacaacgacc cgttcgacgc ttggggtccg 3300
ggtaccgttg ttaccgtttc tccggcgtcg accaaaggtc cgtctgtttt cccgctggct 3360
ccgtcttcta aatctacctc tggtggtacc gctgctctgg gttgcctggt taaagactac 3420
ttcccggaac cggttaccgt ttcttggaac tctggtgctc tgacctctgg tgttcacacc 3480
ttcccggctg ttctgcagtc ttctggtctg tactctctgt cttctgttgt taccgttccg 3540
tcttcttctc tgggtaccca gacctacatc tgcaacgtta accacaaacc gtctaacacc 3600
aaagttgaca agaaagttga accgaaatct tgcctgcgat cgcggccagg ccggccgcac 3660
catcaccatc accatggcgc atacccgtac gacgttccgg actacgcttc tactagttag 3720
aagggtggtg gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc 3780
ggtggtggct ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg 3840
gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat 3900
tctgtcgcta ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt 3960
gctaatggta atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc 4020
ggtgacggtg ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct 4080
caatcggttg aatgtcgccc ttttgtcttt ggcgctggta aaccatatga attttctatt 4140
gattgtgaca aaataaactt attccgtggt gtctttgcgt ttcttttata tgttgccacc 4200
tttatgtatg tattttctac gtttgctaac atactgcgta ataaggagtc ttaagctagc 4260
taacgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag 4320
cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 4380
cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 4440
tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 4500
cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 4560
gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 4620
aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 4680
gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 4740
caaaatatta acgcttacaa tttag                                       4765

<210> 218
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> 3Ala-R Primer

<400> 218
tcgaacgggt ccgcgtccgc cgcacggtca gaacctttac                       40

<210> 219
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> 3Ala-F Primer

<400> 219
gttctgaccg tgcggcggac gcggacccgt tcgacgcttg                       40

<210> 220
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> OmpA-F Primer

<400> 220
gtggcactgg ctggtttcgc tac                                         23

<210> 221
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> VLL1-R Primer

<400> 221
ggaggaagat ccagacgaac cacctttgat ttcaacacgg gtaccctg              48

<210> 222
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> L1VH-F Primer

<400> 222
ggtggctcgg gcggtggtgg cgaagttcag ctggttgaat ctggtg                46

<210> 223
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> VHL2-R Primer

<400> 223
ctgctgctgc tgccggatcc tcccggagaa acggtaacaa cggtac                46

<210> 224
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> L2VH-F Primer

<400> 224
ggcgggagct ccggcggcgg agaagttcag ctggttgaat ctggtg                46

<210> 225
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> VHL1-R Primer

<400> 225
ggaggaagat ccagacgaac cacccggaga aacggtaaca acggtac               47

<210> 226
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> L1VL-F Primer

<400> 226
ggtggctcgg gcggtggtgg cgttgttatg acccagtctc cgtc                  44

<210> 227
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> VLSfi-R Primer

<400> 227
gtgctggccg gcctggcctt tgatttcaac acgggtaccc tg                    42

<210> 228
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> Sfi6His-R Primer

<400> 228
gtgatggtgc tggccggcct ggcctttg                                    28

<210> 229
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> Linker 1(-): (L1 prime)

<400> 229
gccaccaccg cccgagccac cgccaccaga ggcggcagat ccagacgaac cacc       54

<210> 230
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> Linker 2(-): (L2 prime)

<400> 230
tccgccgccg gagctcccgc cgccgccgcc gctgctgctg ctgccggatc ctcc       54

<210> 231
<211> 6819
<212> DNA
<213> Artificial Sequence

<220> 
<223> 2G12 Fab in pET Duet vector

<400> 231
ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag 60
gagatatacc atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac 120
cgtggcccag gcggccgttg ttatgaccca gtctccgtct accctgtctg cttctgttgg 180
tgacaccatc accatcacct gccgtgcttc tcagtctatc gaaacctggc tggcttggta 240
ccagcagaaa ccgggtaaag ctccgaaact gctgatctac aaggcttcta ccctgaaaac 300
cggtgttccg tctcgtttct ctggttctgg ttctggtacc gagttcaccc tgaccatctc 360
tggtctgcag ttcgacgact tcgctaccta ccactgccag cactacgctg gttactctgc 420
taccttcggt cagggtaccc gtgttgaaat caaacgtacc gttgctgctc cgtctgtttt 480
catcttcccg ccgtctgacg aacagctgaa atctggtacc gcttctgttg tttgcctgct 540
gaacaacttc tacccgcgtg aagctaaagt tcagtggaaa gttgacaacg ctctgcagtc 600
tggtaactct caggaatctg ttaccgaaca ggactctaaa gactctacct actctctgtc 660
ttctaccctg accctgtcta aagctgacta cgaaaagcac aaagtttacg cttgcgaagt 720
tacccaccag ggtctgtctt ctccggttac caaatctttc aaccgtggtg aatgctaggg 780
ccaggccggc cgcggccgca taatgcttaa gtcgaacaga aagtaatcgt attgtacacg 840
gccgcataat cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc 900
ccatcttagt atattagtta agtataagaa ggagatatac atatgaaata cctattgcct 960
acggcagccg ctggattgtt attactcgct gcccaaccag ccatggccga agttcagctg 1020
gttgaatctg gtggtggtct ggttaaagct ggtggttctc tgatcctgtc ttgcggtgtt 1080
tctaacttcc gtatctctgc tcacaccatg aactgggttc gtcgtgttcc gggtggtggt 1140
ctggaatggg ttgcttctat ctctacctct tctacctacc gtgactacgc tgacgctgtt 1200
aaaggtcgtt tcaccgtttc tcgtgacgac ctggaagact tcgtttacct gcagatgcat 1260
aaaatgcgtg ttgaagacac cgctatctac tactgcgctc gtaaaggttc tgaccgtctg 1320
tctgacaacg acccgttcga cgcttggggt ccgggtaccg ttgttaccgt ttctccggcg 1380
tcgaccaaag gtccgtctgt tttcccgctg gctccgtctt ctaaatctac ctctggtggt 1440
accgctgctc tgggttgcct ggttaaagac tacttcccgg aaccggttac cgtttcttgg 1500
aactctggtg ctctgacctc tggtgttcac accttcccgg ctgttctgca gtcttctggt 1560
ctgtactctc tgtcttctgt tgttaccgtt ccgtcttctt ctctgggtac ccagacctac 1620
atctgcaacg ttaaccacaa accgtctaac accaaagttg acaagaaagt tgaaccgaaa 1680
tcttgcggca gcagccacca tcaccatcac catggcgcat acccgtacga cgttccggac 1740
tacgcttctt agctcgagtc tggtaaagaa accgctgctg cgaaatttga acgccagcac 1800
atggactcgt ctactagcgc agcttaatta acctaggctg ctgccaccgc tgagcaataa 1860
ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga 1920
actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg 1980
tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 2040
ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 2100
ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 2160
agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 2220
tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 2280
tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 2340
atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt 2400
ctggcggcac gatggcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 2460
aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 2520
atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 2580
ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 2640
tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 2700
agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 2760
taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 2820
tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 2880
cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 2940
ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 3000
tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 3060
tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 3120
cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 3180
tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 3240
gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 3300
tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 3360
atgttgaata ctcatactct tcctttttca atcatgattg aagcatttat cagggttatt 3420
gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggtcatgacc 3480
aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 3540
ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 3600
ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 3660
actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 3720
caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 3780
gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 3840
ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 3900
cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 3960
cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 4020
acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 4080
ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 4140
gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 4200
tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 4260
accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 4320
cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt 4380
gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc 4440
gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg 4500
acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 4560
catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc 4620
atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt 4680
gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt 4740
tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat 4800
gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg 4860
gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa 4920
aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag 4980
ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt 5040
ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga 5100
cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc 5160
agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgctagt 5220
catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg 5280
agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc 5340
tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 5400
aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca 5460
gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt 5520
gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt 5580
cttcggtatc gtcgtatccc actaccgaga tgtccgcacc aacgcgcagc ccggactcgg 5640
taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa 5700
cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc 5760
cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca 5820
gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac 5880
ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac 5940
tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag 6000
cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc 6060
gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca 6120
tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt 6180
gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc 6240
ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca 6300
ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct 6360
gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca 6420
ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt 6480
cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc 6540
agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg 6600
gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc 6660
atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca 6720
gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag 6780
atcgatctcg atcccgcgaa attaatacga ctcactata                        6819


<210> 232
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> VHSfi-R  Primer

<400> 232
ccatggtgat ggtgatggtg ctggccggcc tggcccggag aaacggtaac aacggtac   58

<210> 233
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> AgeI-F Primer

<400> 233
ccctgaaaac cggtgttccg tctc                                        24

<210> 234
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Cys19-R Primer

<400> 234
caccgcaaga caggcacaga gaaccaccag                                  30

<210> 235
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Cys19-F Primer

<400> 235
ctggtggttc tctgtgcctg tcttgcggtg                                  30

<210> 236
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> NcoI25- R Primer

<400> 236
ggtatgcgcc atggtgatgg tgatg                                       25

<210> 237
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> VHhinge- F Primer
      

<400> 237
ccgtttctcc gccgaaaagc tgcgataaaa cccatacctg cc                    42

<210> 238
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> HingeTemplate- F Primer
      

<400> 238
gctgcgataa aacccatacc tgcccgccgt gcccgggcca g                     41

<210> 239
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> HingeTemplate- R Primer

<400> 239
gatggtgatg gtgctggccg gcctggcccg ggcacggcgg gcag                  44

<210> 240
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> NcoI38- R Primer

<400> 240
gcggcgccat ggtgatggtg atggtgctgg ccggcctg                         38

<210> 241
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> HingeVH(E)- R Primer

<400> 241
cgcagctttt cggttccgga gaaacggtaa caacggtacc cggac                 45

<210> 242
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> VHhinge(E)- F Primer

<400> 242
ccgtttctcc ggaaccgaaa agctgcgata aaacccatac ctgcc                 45

<210> 243
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> NdeIVH- F Primer

<400> 243
ggagatatac atatgaaata cctattgcct ac                               32

<210> 244
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> XhoIHA26- R Primer

<400> 244
taccagactc gagctaagaa gcgtag                                      26

<210> 245
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> HingeCH1- R Primer

<400> 245
caggtatggg ttttatcgca gcttttcggt tcaactttct tgtc                  44

<210> 246
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> HingeCH1- R Primer

<400> 246
caggtatggg ttttatcgca gcttttcggt tcaactttct tgtc                  44

<210> 247
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> CH1Hinge- F Primer

<400> 247
ccgaaaagct gcgataaaac ccatacctgc ccgccgtgc                        39

<210> 248
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> HingeHisTemplate- F Primer

<400> 248
cccatacctg cccgccgtgc ccgcaccatc accatcacca tggcg                 45

<210> 249
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> HingeHisTemplate- R Primer

<400> 249
gtccggaacg tcgtacgggt atgcgccatg gtgatggtga tggtgcg               47

<210> 250
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> XhoIHA- R Primer

<400> 250
accagactcg agctaagaag cgtagtccgg aacgtcgtac gggtatg               47

<210> 251
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> XbaIVL-F Primer

<400> 251
ggggaattgt gagcggataa caattc                                      26

<210> 252
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> BamHICK-R Primer

<400> 252
ccgccaccgg atccaccacc agattcacca cggttgaaag atttggtaac c          51

<210> 253
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> SacIVH-F Primer

<400> 253
gcggtgggag ctccggtgaa gttcagctgg ttgaatctgg tg                    42

<210> 254
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> HingeCH1deltaC-R Primer

<400> 254
ctggccggcc tggccgctgc tgccagattt cggttcaact ttcttgtcaa c          51

<210> 255
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> NcoIHinge-R Primer

<400> 255
gtatgcgcca tggtgatggt gatggtgctg gccggcctgg ccgctg                46

<210> 256
<211> 79
<212> DNA
<213> Artificial Sequence

<220> 
<223> SacIBamHI(-) Primer

<400> 256
cccaccgcta ccgccgcctt cgctgccgcc accttcgcta ccgccacctt cgctgccacc 60
accttcgctg ccgccaccg                                              79

<210> 257
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> L216F Primer

<400> 257
gatccggcag cagcagcagc ggcggcggga gct                              33

<210> 258
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> L216R Primer

<400> 258
cccgccgccg ctgctgctgc tgccg                                       25

<210> 259
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> L217F Primer

<400> 259
gatccggcag cagcagcagc ggcggcggcg ggagct                           36

<210> 260
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> L217R Primer

<400> 260
cccgccgccg ccgctgctgc tgctgccg                                    28

<210> 261
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> L219F Primer

<400> 261
gatccagcgg cagcagcagc agcggcggcg gcggcgggag ct                    42

<210> 262
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> L219R Primer

<400> 262
cccgccgccg ccgccgctgc tgctgctgcc gctg                             34

<210> 263
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> L220F Primer

<400> 263
gatccagcgg cggcagcagc agcagcggcg gcggcggcgg gagct                 45

<210> 264
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> L220R Primer

<400> 264
cccgccgccg ccgccgctgc tgctgctgcc gccgctg                          37

<210> 265
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reference sequence used to design H1F

<400> 265
aacttccgta tctctgctca caccatgaac tgggttcgt                        39

<210> 266
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reference sequence used to design H1R

<400> 266
acgacgaacc cagttcatgg tgtgagcaga gatacggaa                        39

<210> 267
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reference sequence used to design H3F

<400> 267
tactactgcg ctcgtaaagg ttctgaccgt ctgtctgaca acgacccgtt cgacgcttgg 60


<210> 268
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reference sequence used to design H3R

<400> 268
accccaagcg tcgaacgggt cgttgtcaga cagacggtca gaacctttac gagcgcagta 60


<210> 269
<211> 225
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2G12 Fab VH-CH1

<400> 269
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Ala Gly Gly
 1               5                  10                  15      
Ser Leu Ile Leu Ser Cys Gly Val Ser Asn Phe Arg Ile Ser Ala His
            20                  25                  30          
Thr Met Asn Trp Val Arg Arg Val Pro Gly Gly Gly Leu Glu Trp Val
        35                  40                  45              
Ala Ser Ile Ser Thr Ser Ser Thr Tyr Arg Asp Tyr Ala Asp Ala Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Val Ser Arg Asp Asp Leu Glu Asp Phe Val Tyr
65                  70                  75                  80  
Leu Gln Met His Lys Met Arg Val Glu Asp Thr Ala Ile Tyr Tyr Cys
                85                  90                  95      
Ala Arg Lys Gly Ser Asp Arg Leu Ser Asp Asn Asp Pro Phe Asp Ala
            100                 105                 110         
Trp Gly Pro Gly Thr Val Val Thr Val Ser Pro Ala Ser Thr Lys Gly
        115                 120                 125             
Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly
    130                 135                 140                 
Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val
145                 150                 155                 160 
Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe
                165                 170                 175     
Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val
            180                 185                 190         
Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val
        195                 200                 205             
Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys
    210                 215                 220                 
Ser
225 


<210> 270
<211> 212
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2G12 Fab VL

<400> 270
Val Val Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly Asp
 1               5                  10                  15      
Thr Ile Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Glu Thr Trp Leu
            20                  25                  30          
Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr
        35                  40                  45              
Lys Ala Ser Thr Leu Lys Thr Gly Val Pro Ser Arg Phe Ser Gly Ser
    50                  55                  60                  
Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Gly Leu Gln Phe Asp
65                  70                  75                  80  
Asp Phe Ala Thr Tyr His Cys Gln His Tyr Ala Gly Tyr Ser Ala Thr
                85                  90                  95      
Phe Gly Gln Gly Thr Arg Val Glu Ile Lys Arg Thr Val Ala Ala Pro
            100                 105                 110         
Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr
        115                 120                 125             
Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys
    130                 135                 140                 
Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu
145                 150                 155                 160 
Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser
                165                 170                 175     
Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala
            180                 185                 190         
Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe
        195                 200                 205             
Asn Arg Gly Glu
    210         


<210> 271
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> reverse complement Sap-I cleavage site

<400> 271
gaagagc                                                           7

<210> 272
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> Pel B leader

<400> 272
atgaaatacc tgctgccgac cgcagccgct ggtctgctgc tgctcgcggc ccagccggcc 60
atggccgccg gt                                                     72

<210> 273
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> Pel B leader

<400> 273
Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala
 1               5                  10                  15      
Ala Gln Pro Ala Met Ala Ala Gly
            20                  


<210> 274
<211> 72
<212> DNA
<213> Artificial Sequence

<220> 
<223> Pel B leader amber stop

<400> 274
atgaaatacc tgctgccgac cgcagccgct ggtctgctgc tgctcgcggc ctagccggcc 60
atggccgccg gt                                                     72

<210> 275
<211> 17
<212> PRT
<213> Artificial Sequence

<220> 
<223> Pel B leader amber stop

<400> 275
Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala
 1               5                  10                  15      
Ala
    


<210> 276
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> OmpA leader

<400> 276
atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcccag 60
gcggccgca                                                         69

<210> 277
<211> 23
<212> PRT
<213> Artificial Sequence

<220> 
<223> OmpA leader

<400> 277
Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala
 1               5                  10                  15      
Thr Val Ala Gln Ala Ala Ala
            20              


<210> 278
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> OmpA leader amber stop

<400> 278
atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcctag 60
gcggccgca                                                         69

<210> 279
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> OmpA leader amber stop

<400> 279
Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala
 1               5                  10                  15      
Thr Val Ala
            


<210> 280
<211> 5882
<212> DNA
<213> Artificial Sequence

<220> 
<223> 2G12 pCAL IT*

<400> 280
gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 60
caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 120
ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 180
gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 240
tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 300
ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 360
tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 420
atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 480
gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 540
caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 600
ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 660
ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 720
ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 780
ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 840
gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 900
ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 960
taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1020
agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1080
atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1140
aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200
caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1260
ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1320
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1380
tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1440
gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1500
ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1560
gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1620
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1680
ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1740
tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1800
ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1860
agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1920
aagcgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga 1980
gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc 2040
cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 2100
aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 2160
acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 2220
gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 2280
cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 2340
tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 2400
cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 2460
gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 2520
tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 2580
ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 2640
agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct 2700
gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc 2760
aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata 2820
cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt 2880
tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt 2940
gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa 3000
tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 3060
ttcccgactg gaaagcgggc agtgagcggt acccgataaa agcggcttcc tgacaggagg 3120
ccgttttgtt ttgcagccca cctcaacgca attaatgtga gttagctcac tcattaggca 3180
ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 3240
caattgaatt aaggaggata taattatgaa atacctgctg ccgaccgcag ccgctggtct 3300
gctgctgctc gcggcctagc cggccatggc cgccggtgtt gttatgaccc agtctccgtc 3360
taccctgtct gcttctgttg gtgacaccat caccatcacc tgccgtgctt ctcagtctat 3420
cgaaacctgg ctggcttggt accagcagaa accgggtaaa gctccgaaac tgctgatcta 3480
caaggcttct accctgaaaa ccggtgttcc gtctcgtttc tctggttctg gttctggtac 3540
cgagttcacc ctgaccatct ctggtctgca gttcgacgac ttcgctacct accactgcca 3600
gcactacgct ggttactctg ctaccttcgg tcagggtacc cgtgttgaaa tcaaacgtac 3660
cgttgctgct ccgtctgttt tcatcttccc gccgtctgac gaacagctga aatctggtac 3720
cgcttctgtt gtttgcctgc tgaacaactt ctacccgcgt gaagctaaag ttcagtggaa 3780
agttgacaac gctctgcagt ctggtaactc tcaggaatct gttaccgaac aggactctaa 3840
agactctacc tactctctgt cttctaccct gaccctgtct aaagctgact acgaaaagca 3900
caaagtttac gcttgcgaag ttacccacca gggtctgtct tctccggtta ccaaatcttt 3960
caaccgtggt gaatgctaat taattaataa ggaggatata attatgaaaa agacagctat 4020
cgcgattgca gtggcactgg ctggtttcgc taccgtagcc taggcggccg cagaagttca 4080
gctggttgaa tctggtggtg gtctggttaa agctggtggt tctctgatcc tgtcttgcgg 4140
tgtttctaac ttccgtatct ctgctcacac catgaactgg gttcgtcgtg ttccgggtgg 4200
tggtctggaa tgggttgctt ctatctctac ctcttctacc taccgtgact acgctgacgc 4260
tgttaaaggt cgtttcaccg tttctcgtga cgacctggaa gacttcgttt acctgcagat 4320
gcataaaatg cgtgttgaag acaccgctat ctactactgc gctcgtaaag gttctgaccg 4380
tctgtctgac aacgacccgt tcgacgcttg gggtccgggt accgttgtta ccgtttctcc 4440
ggcgtcgacc aaaggtccgt ctgttttccc gctggctccg tcttctaaat ctacctctgg 4500
tggtaccgct gctctgggtt gcctggttaa agactacttc ccggaaccgg ttaccgtttc 4560
ttggaactct ggtgctctga cctctggtgt tcacaccttc ccggctgttc tgcagtcttc 4620
tggtctgtac tctctgtctt ctgttgttac cgttccgtct tcttctctgg gtacccagac 4680
ctacatctgc aacgttaacc acaaaccgtc taacaccaaa gttgacaaga aagttgaacc 4740
gaaatcttgc ctgcgatcgc ggccaggccg gccgcaccat caccatcacc atggcgcata 4800
cccgtacgac gttccggact acgcttctac tagttaggag ggtggtggct ctgagggtgg 4860
cggttctgag ggtggcggct ctgagggagg cggttccggt ggtggctctg gttccggtga 4920
ttttgattat gaaaagatgg caaacgctaa taagggggct atgaccgaaa atgccgatga 4980
aaacgcgcta cagtctgacg ctaaaggcaa acttgattct gtcgctactg attacggtgc 5040
tgctatcgat ggtttcattg gtgacgtttc cggccttgct aatggtaatg gtgctactgg 5100
tgattttgct ggctctaatt cccaaatggc tcaagtcggt gacggtgata attcaccttt 5160
aatgaataat ttccgtcaat atttaccttc cctccctcaa tcggttgaat gtcgcccttt 5220
tgtctttggc gctggtaaac catatgaatt ttctattgat tgtgacaaaa taaacttatt 5280
ccgtggtgtc tttgcgtttc ttttatatgt tgccaccttt atgtatgtat tttctacgtt 5340
tgctaacata ctgcgtaata aggagtctta agctagctaa cgatcgccct tcccaacagt 5400
tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg 5460
tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 5520
ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 5580
ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 5640
agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 5700
tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 5760
tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 5820
atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt 5880
ag                                                                5882


<210> 281
<211> 5882
<212> DNA
<213> Artificial Sequence

<220> 
<223> 2G12 pCAL ITPO vector

<400> 281
gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 60
caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 120
ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 180
gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 240
tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 300
ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 360
tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 420
atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 480
gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 540
caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 600
ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 660
ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 720
ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 780
ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 840
gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 900
ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 960
taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1020
agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1080
atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1140
aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200
caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1260
ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1320
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1380
tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1440
gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1500
ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1560
gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1620
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1680
ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1740
tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1800
ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1860
agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1920
aagcgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga 1980
gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc 2040
cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 2100
aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 2160
acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 2220
gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 2280
cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 2340
tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 2400
cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 2460
gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 2520
tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 2580
ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 2640
agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct 2700
gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc 2760
aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata 2820
cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt 2880
tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt 2940
gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa 3000
tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 3060
ttcccgactg gaaagcgggc agtgagcggt acccgataaa agcggcttcc tgacaggagg 3120
ccgttttgtt ttgcagccca cctcaacgca attaatgtga gttagctcac tcattaggca 3180
ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 3240
caattgaatt aaggaggata taattatgaa atacctgctg ccgaccgcag ccgctggtct 3300
gctgctgctc gcggcccagc cggccatggc cgccggtgtt gttatgaccc agtctccgtc 3360
taccctgtct gcttctgttg gtgacaccat caccatcacc tgccgtgctt ctcagtctat 3420
cgaaacctgg ctggcttggt accagcagaa accgggtaaa gctccgaaac tgctgatcta 3480
caaggcttct accctgaaaa ccggtgttcc gtctcgtttc tctggttctg gttctggtac 3540
cgagttcacc ctgaccatct ctggtctgca gttcgacgac ttcgctacct accactgcca 3600
gcactacgct ggttactctg ctaccttcgg tcagggtacc cgtgttgaaa tcaaacgtac 3660
cgttgctgct ccgtctgttt tcatcttccc gccgtctgac gaacagctga aatctggtac 3720
cgcttctgtt gtttgcctgc tgaacaactt ctacccgcgt gaagctaaag ttcagtggaa 3780
agttgacaac gctctgcagt ctggtaactc tcaggaatct gttaccgaac aggactctaa 3840
agactctacc tactctctgt cttctaccct gaccctgtct aaagctgact acgaaaagca 3900
caaagtttac gcttgcgaag ttacccacca gggtctgtct tctccggtta ccaaatcttt 3960
caaccgtggt gaatgctaat taattaataa ggaggatata attatgaaaa agacagctat 4020
cgcgattgca gtggcactgg ctggtttcgc taccgtagcc caggcggccg cagaagttca 4080
gctggttgaa tctggtggtg gtctggttaa agctggtggt tctctgatcc tgtcttgcgg 4140
tgtttctaac ttccgtatct ctgctcacac catgaactgg gttcgtcgtg ttccgggtgg 4200
tggtctggaa tgggttgctt ctatctctac ctcttctacc taccgtgact acgctgacgc 4260
tgttaaaggt cgtttcaccg tttctcgtga cgacctggaa gacttcgttt acctgcagat 4320
gcataaaatg cgtgttgaag acaccgctat ctactactgc gctcgtaaag gttctgaccg 4380
tctgtctgac aacgacccgt tcgacgcttg gggtccgggt accgttgtta ccgtttctcc 4440
ggcgtcgacc aaaggtccgt ctgttttccc gctggctccg tcttctaaat ctacctctgg 4500
tggtaccgct gctctgggtt gcctggttaa agactacttc ccggaaccgg ttaccgtttc 4560
ttggaactct ggtgctctga cctctggtgt tcacaccttc ccggctgttc tgcagtcttc 4620
tggtctgtac tctctgtctt ctgttgttac cgttccgtct tcttctctgg gtacccagac 4680
ctacatctgc aacgttaacc acaaaccgtc taacaccaaa gttgacaaga aagttgaacc 4740
gaaatcttgc ctgcgatcgc ggccaggccg gccgcaccat caccatcacc atggcgcata 4800
cccgtacgac gttccggact acgcttctac tagttaggag ggtggtggct ctgagggtgg 4860
cggttctgag ggtggcggct ctgagggagg cggttccggt ggtggctctg gttccggtga 4920
ttttgattat gaaaagatgg caaacgctaa taagggggct atgaccgaaa atgccgatga 4980
aaacgcgcta cagtctgacg ctaaaggcaa acttgattct gtcgctactg attacggtgc 5040
tgctatcgat ggtttcattg gtgacgtttc cggccttgct aatggtaatg gtgctactgg 5100
tgattttgct ggctctaatt cccaaatggc tcaagtcggt gacggtgata attcaccttt 5160
aatgaataat ttccgtcaat atttaccttc cctccctcaa tcggttgaat gtcgcccttt 5220
tgtctttggc gctggtaaac catatgaatt ttctattgat tgtgacaaaa taaacttatt 5280
ccgtggtgtc tttgcgtttc ttttatatgt tgccaccttt atgtatgtat tttctacgtt 5340
tgctaacata ctgcgtaata aggagtctta agctagctaa cgatcgccct tcccaacagt 5400
tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg 5460
tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 5520
ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 5580
ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 5640
agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 5700
tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 5760
tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 5820
atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt 5880
ag                                                                5882


<210> 282
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> LacITerm-F1 Primer

<400> 282
ggcgccgctc ttcgagcgac accatcgaat ggcgcaaaac c                     41

<210> 283
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> LacITerm-R1 Primer

<400> 283
cttttatcgg gtaccgctca ctgcccgctt tccagtcggg a                     41

<210> 284
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> Term-R Primer

<400> 284
gctaggtggg ctgcaaaaca aaacggcctc ctgtcaggaa gccgctttta tcgggtac   58

<210> 285
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> LacITerm-F2 Primer

<400> 285
aaagcgggca gtgagcggta cccgataaaa gcggcttcct g                     41

<210> 286
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> TermPO-R Primer

<400> 286
cacattaatt gcgttgaggt gggctgcaaa acaaaacggc c                     41

<210> 287
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> TermPO-F Primer

<400> 287
gttttgcagc ccacctcaac gcaattaatg tgagttagct c                     41

<210> 288
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> SgrAIPelB-R Primer

<400> 288
cataacaaca ccggcggcca tggccggctg                                  30

<210> 289
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> SeqpCALTerm-F Primer

<400> 289
taaccgtatt accgcctttg                                             20

<210> 290
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> SeqpCALTerm-R Primer

<400> 290
tgccagctgc attaatgaat                                             20

<210> 291
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> SeqpCALIT-R Primer

<400> 291
cataactcac attaattgcg t                                           21

<210> 292
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> SeqITPO-F2 Primer

<400> 292
gttgcccgtc tcactggtga                                             20

<210> 293
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> KasI-F Primer

<400> 293
ccaccctggc gcccaatacg caaac                                       25

<210> 294
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> AmbPelB-R Primer

<400> 294
gcggccatgg ccggctaggc cgcgagcagc agcagac                          37

<210> 295
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> AmbPelB-F Primer

<400> 295
tgctgctcgc ggcctagccg gccatggccg ccggtgt                          37

<210> 296
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> AmbOmpA-R Primer

<400> 296
gaacttctgc ggccgcctag gctacggtag cgaaac                           36

<210> 297
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> SeqHCFR1-R Primer

<400> 297
ttagaaacac cgcaagacag                                             20

<210> 298
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> SeqpCAL-F Primer

<400> 298
atataattat gaaatacctg                                             20

<210> 299
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> SeqITPO-F4 Primer

<400> 299
gcgtggaccg cttgctgcaa                                             20




2

