                         SEQUENCE LISTING

<110>  CANNON, Paula
 
<120>  METHODS TO IDENTIFY MODULATORS OF ANTI-TETHERIN ACTIVITY TO 
       INHIBIT PROPAGATION OF VIRUSES

<130>  CHLA-106-PCT

<150>  61/191,151
<151>  2008-09-04

<150>  61/196,291
<151>  2008-10-16

<160>  13    

<170>  PatentIn version 3.4

<210>  1
<211>  180
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Ser Thr Ser Tyr Asp Tyr Cys Arg Val Pro Met Glu Asp Gly 
1               5                   10                  15      


Asp Lys Arg Cys Lys Leu Leu Leu Gly Ile Gly Ile Leu Val Leu Leu 
            20                  25                  30          


Ile Ile Val Ile Leu Gly Val Pro Leu Ile Ile Phe Thr Ile Lys Ala 
        35                  40                  45              


Asn Ser Glu Ala Cys Arg Asp Gly Leu Arg Ala Val Met Glu Cys Arg 
    50                  55                  60                  


Asn Val Thr His Leu Leu Gln Gln Glu Leu Thr Glu Ala Gln Lys Gly 
65                  70                  75                  80  


Phe Gln Asp Val Glu Ala Gln Ala Ala Thr Cys Asn His Thr Val Met 
                85                  90                  95      


Ala Leu Met Ala Ser Leu Asp Ala Glu Lys Ala Gln Gly Gln Lys Lys 
            100                 105                 110         


Val Glu Glu Leu Glu Gly Glu Ile Thr Thr Leu Asn His Lys Leu Gln 
        115                 120                 125             


Asp Ala Ser Ala Glu Val Glu Arg Leu Arg Arg Glu Asn Gln Val Leu 
    130                 135                 140                 


Ser Val Arg Ile Ala Asp Lys Lys Tyr Tyr Pro Ser Ser Gln Asp Ser 
145                 150                 155                 160 


Ser Ser Ala Ala Ala Pro Gln Leu Leu Ile Val Leu Leu Gly Leu Ser 
                165                 170                 175     


Ala Leu Leu Gln 
            180 


<210>  2
<211>  180
<212>  PRT
<213>  Chlorocebus aethiops

<400>  2

Met Ala Ser Thr Ser Tyr Asp Tyr Cys Arg Val Pro Met Glu Asp Gly 
1               5                   10                  15      


Asp Lys Arg Cys Lys Leu Leu Leu Gly Ile Gly Ile Leu Val Leu Leu 
            20                  25                  30          


Ile Ile Val Ile Leu Gly Val Pro Leu Ile Ile Phe Thr Ile Lys Ala 
        35                  40                  45              


Asn Ser Glu Ala Cys Arg Asp Gly Leu Arg Ala Val Met Glu Cys Arg 
    50                  55                  60                  


Asn Val Thr His Leu Leu Gln Gln Glu Leu Thr Glu Ala Gln Lys Gly 
65                  70                  75                  80  


Phe Gln Asp Val Glu Ala Gln Ala Ala Thr Cys Asn His Thr Val Met 
                85                  90                  95      


Ala Leu Met Ala Ser Leu Asp Ala Glu Lys Ala Gln Gly Gln Lys Lys 
            100                 105                 110         


Val Glu Glu Leu Glu Gly Glu Ile Thr Thr Leu Asn His Lys Leu Gln 
        115                 120                 125             


Asp Ala Ser Ala Glu Val Glu Arg Leu Arg Arg Glu Asn Gln Val Leu 
    130                 135                 140                 


Ser Val Arg Ile Ala Asp Lys Lys Tyr Tyr Pro Ser Ser Gln Asp Ser 
145                 150                 155                 160 


Ser Ser Ala Ala Ala Pro Gln Leu Leu Ile Val Leu Leu Gly Leu Ser 
                165                 170                 175     


Ala Leu Leu Gln 
            180 


<210>  3
<211>  182
<212>  PRT
<213>  Macaque fasicularis

<400>  3

Met Ala Pro Ile Leu Tyr Asp Tyr Cys Lys Met Pro Met Asp Asp Ile 
1               5                   10                  15      


Trp Lys Glu Asp Gly Asp Lys Arg Cys Lys Pro Val Ile Gly Ile Leu 
            20                  25                  30          


Gly Leu Leu Val Ile Val Leu Leu Gly Val Leu Leu Ile Phe Phe Thr 
        35                  40                  45              


Ile Lys Ala Asn Ser Glu Ala Cys Gln Asp Gly Leu Arg Ala Val Met 
    50                  55                  60                  


Glu Cys Arg Asn Val Thr Tyr Leu Leu Gln Gln Glu Leu Ala Glu Ala 
65                  70                  75                  80  


Gln Arg Gly Phe Arg Asp Ala Glu Ala Gln Ala Val Thr Cys Asn Gln 
                85                  90                  95      


Thr Val Met Ala Leu Met Ala Ser Leu Asp Ala Glu Lys Ala Gln Gly 
            100                 105                 110         


Arg Lys Lys Val Glu Glu Leu Glu Gly Glu Ile Thr Thr Leu Asn His 
        115                 120                 125             


Lys Leu Gln Asp Ala Ser Ala Glu Val Glu Arg Leu Arg Arg Glu Asn 
    130                 135                 140                 


His Val Leu Asn Ala Arg Ile Ala Asp Thr Asp Ser Ala Ser Ser Gln 
145                 150                 155                 160 


Asp Ser Ser Cys Ala Ala Glu Pro Pro Leu Leu Ile Leu Leu Leu Gly 
                165                 170                 175     


Leu Ser Ala Leu Leu Leu 
            180         


<210>  4
<211>  181
<212>  PRT
<213>  Macaque mulatta

<400>  4

Met Ala Pro Ile Leu Tyr Asp Tyr Cys Lys Met Pro Met Asp Asp Ile 
1               5                   10                  15      


Trp Lys Glu Asp Gly Asp Lys Arg Cys Lys Leu Val Ile Gly Ile Leu 
            20                  25                  30          


Gly Leu Leu Val Ile Val Leu Leu Gly Val Leu Leu Ile Phe Phe Thr 
        35                  40                  45              


Ile Lys Ala Asn Ser Glu Ala Cys Gln Asp Gly Leu Arg Ala Val Met 
    50                  55                  60                  


Glu Cys Arg Asn Val Thr Tyr Leu Leu Gln Gln Glu Leu Ala Glu Ala 
65                  70                  75                  80  


Gln Arg Gly Phe Arg Asp Ala Glu Ala Gln Ala Val Thr Cys Asn Gln 
                85                  90                  95      


Thr Val Met Ala Leu Met Ala Ser Leu Asp Ala Glu Lys Ala Gln Gly 
            100                 105                 110         


Arg Lys Lys Val Glu Glu Leu Glu Gly Glu Ile Thr Thr Leu Asn His 
        115                 120                 125             


Lys Leu Gln Asp Ser Ala Glu Val Glu Arg Leu Arg Arg Glu Asn His 
    130                 135                 140                 


Val Leu Asn Ala Arg Ile Ala Asp Thr Asp Ser Gly Ser Ser Gln Asp 
145                 150                 155                 160 


Ser Ser Cys Ala Ala Glu Pro Pro Val Leu Ile Leu Leu Leu Gly Leu 
                165                 170                 175     


Ser Ala Leu Leu Leu 
            180     


<210>  5
<211>  181
<212>  PRT
<213>  Macaque nemistrina

<400>  5

Met Ala Pro Ile Leu Tyr Asp Tyr Cys Lys Met Pro Met Asp Asp Ile 
1               5                   10                  15      


Trp Lys Glu Asp Gly Asp Lys Arg Cys Lys Leu Val Val Gly Ile Leu 
            20                  25                  30          


Gly Leu Leu Val Ile Val Leu Leu Gly Val Leu Leu Ile Phe Phe Thr 
        35                  40                  45              


Ile Lys Ala Asn Ser Glu Ala Cys Gln Asp Gly Leu Arg Ala Val Met 
    50                  55                  60                  


Glu Cys Arg Asn Val Thr Tyr Leu Leu Gln Gln Glu Leu Ala Glu Ala 
65                  70                  75                  80  


Gln Arg Gly Phe Arg Asp Ala Glu Ala Gln Ala Val Thr Cys Asn Gln 
                85                  90                  95      


Thr Val Met Ala Leu Met Ala Ser Leu Asp Ala Glu Lys Ala Gln Gly 
            100                 105                 110         


Arg Lys Lys Val Glu Glu Leu Glu Gly Glu Ile Thr Thr Leu Asn Asp 
        115                 120                 125             


Lys Leu Gln Asp Ser Ala Glu Val Glu Arg Leu Arg Arg Glu Asn His 
    130                 135                 140                 


Val Leu Asn Ala Arg Ile Ala Asp Thr Asp Ser Ala Ser Ser Gln Asp 
145                 150                 155                 160 


Ser Ser Cys Ala Ala Glu Pro Pro Leu Leu Ile Leu Leu Leu Gly Leu 
                165                 170                 175     


Ser Ala Leu Leu Leu 
            180     


<210>  6
<211>  184
<212>  PRT
<213>  Pan troglodytes

<400>  6

Met Ala Ser Thr Leu Tyr Asp Tyr Cys Arg Val Pro Met Asp Asp Ile 
1               5                   10                  15      


Trp Lys Lys Asp Gly Asp Lys Arg Cys Lys Leu Leu Leu Gly Ile Gly 
            20                  25                  30          


Ile Leu Met Leu Leu Ile Ile Val Ile Leu Gly Val Pro Leu Ile Ile 
        35                  40                  45              


Phe Thr Ile Lys Ala Asn Ser Glu Ala Cys Arg Asp Gly Leu Arg Ala 
    50                  55                  60                  


Val Met Glu Cys Arg Asn Val Thr His Leu Leu Gln Gln Glu Leu Thr 
65                  70                  75                  80  


Glu Ala Gln Lys Gly Phe Gln Asp Val Glu Ala Gln Ala Ala Thr Cys 
                85                  90                  95      


Asn His Thr Val Met Ala Leu Met Ala Ser Leu Asp Ala Glu Lys Ala 
            100                 105                 110         


Gln Gly Gln Lys Lys Val Glu Glu Leu Glu Glu Glu Ile Thr Thr Leu 
        115                 120                 125             


Asn His Lys Leu Gln Asp Ser Ala Glu Val Glu Arg Leu Arg Arg Glu 
    130                 135                 140                 


Asn Gln Val Leu Ser Val Arg Ile Ala Asp Lys Lys Tyr Tyr Ser Ser 
145                 150                 155                 160 


Ser Gln Asp Ser Ser Ser Ala Ala Ala Pro Gln Leu Leu Ile Val Leu 
                165                 170                 175     


Leu Gly Leu Ser Ala Leu Leu Gln 
            180                 


<210>  7
<211>  983
<212>  DNA
<213>  Homo sapiens

<400>  7
gtggaattca tggcatctac ttcgtatgac tattgcagag tgcccatgga agacggggat     60

aagcgctgta agcttctgct ggggatagga attctggtgc tcctgatcat cgtgattctg    120

ggggtgccct tgattatctt caccatcaag gccaacagcg aggcctgccg ggacggcctt    180

cgggcagtga tggagtgtcg caatgtcacc catctcctgc aacaagagct gaccgaggcc    240

cagaagggct ttcaggatgt ggaggcccag gccgccacct gcaaccacac tgtgatggcc    300

ctaatggctt ccctggatgc agagaaggcc caaggacaaa agaaagtgga ggagcttgag    360

ggagagatca ctacattaaa ccataagctt caggacgcgt ctgcagaggt ggagcgactg    420

agaagagaaa accaggtctt aagcgtgaga atcgcggaca agaagtacta ccccagctcc    480

caggactcca gctccgctgc ggcgccccag ctgctgattg tgctgctggg cctcagcgct    540

ctgctgcagt gagatcccag gaagctggca catcttggaa ggtccgtcct gctcggcttt    600

tcgcttgaac attcccttga tctcatcagt tctgagcggg tcatggggca acacggttag    660

cggggagagc acggggtagc cggagaaggg cctctggagc aggtctggag gggccatggg    720

gcagtcctgg gtgtggggac acagtcgggt tgacccaggg ctgtctccct ccagagcctc    780

cctccggaca atgagtcccc cctcttgtct cccaccctga gattgggcat ggggtgcggt    840

gtggggggca tgtgctgcct gttgttatgg gttttttttg cggggggggt tgcttttttc    900

tggggtcttt gagctccaaa aaataaacac ttcctttgag ggagagcaaa aaaaaaaaaa    960

aaaaaaaaaa aaaaaaaaaa aaa                                            983


<210>  8
<211>  66
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Consensus nucleic acid sequence for transmembrane domain of 
       Tetherins.


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nnn at nucleotides 1-3 can encode a leucine or no amino acid

<220>
<221>  misc_feature
<222>  (4)..(6)
<223>  nnn at nucleotides 4-6 can encode a leucine or no amino acid

<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  nnn at nucleotides 7-9 can encode a leucine or proline

<220>
<221>  misc_feature
<222>  (10)..(12)
<223>  nnn at nucleotides 10-12 can encode a hydrophobic amino acid

<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  nnn at nucleotides 13-15 can encode isoleucine or valine

<220>
<221>  misc_feature
<222>  (25)..(27)
<223>  nnn at nucleotides 25-27 can enode a hydrophobic amino acid

<220>
<221>  misc_feature
<222>  (34)..(36)
<223>  nnn at nucleotides 34-36 can encode an isoleucine or valine

<220>
<221>  misc_feature
<222>  (43)..(45)
<223>  nnn at nucleotides 43-45 can encode an isoleucine or leucine

<220>
<221>  misc_feature
<222>  (55)..(57)
<223>  nnn at nucleotides 55-57 can encode a proline or leucine

<220>
<221>  misc_feature
<222>  (64)..(66)
<223>  nnn at nucleotides 64-66 can encode an isoleucine or 
       phenylalanine

<400>  8
nnnnnnnnnn nnnnnggaat tctgnnnctc ctgnnnatcg tgnnnctggg ggtgnnnttg     60

attnnn                                                                66


<210>  9
<211>  144
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Consensus nucleic acid sequence for Tetherin transmembrane domain
       and cytoplasmic tail


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  nnn at positions 7-9 can encode proline or serine

<220>
<221>  misc_feature
<222>  (10)..(12)
<223>  nnn at positions 10-12 can encode threonine or isoleucine

<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  nnn at positions 13-15 can encode serine or leucine

<220>
<221>  misc_feature
<222>  (25)..(27)
<223>  nnn at positions 25-27 can encode cysteine or arginine

<220>
<221>  misc_feature
<222>  (28)..(30)
<223>  nnn at positions 28-30 can encode a positively charged residue

<220>
<221>  misc_feature
<222>  (31)..(33)
<223>  nnn at positions 31-33 can encode a hydrophobic residue

<220>
<221>  misc_feature
<222>  (40)..(42)
<223>  nnn at positions 40-42 can encode an aspartic acid or no residue

<220>
<221>  misc_feature
<222>  (43)..(45)
<223>  nnn at positions 43-45 can encode an aspartic acid or no residue

<220>
<221>  misc_feature
<222>  (46)..(48)
<223>  nnn at positions 46-48 can encode an isoleucine or no residue

<220>
<221>  misc_feature
<222>  (49)..(51)
<223>  nnn at positions 49-51 can encode a cysteine, a tryptophan, or no
       residue

<220>
<221>  misc_feature
<222>  (52)..(54)
<223>  nnn at positions 52-54 can encode a lysine or no residue

<220>
<221>  misc_feature
<222>  (55)..(57)
<223>  nnn at positions 55-57 can encode a charged residue

<220>
<221>  misc_feature
<222>  (61)..(63)
<223>  nnn at positions 61-63 can encode a glycine or an arginine

<220>
<221>  misc_feature
<222>  (70)..(72)
<223>  nnn at positions 70-72 can encode an arginine or cysteine

<220>
<221>  misc_feature
<222>  (79)..(81)
<223>  nnn at positions 79-81 can encode a leucine or no amino acid

<220>
<221>  misc_feature
<222>  (82)..(84)
<223>  nnn at positions 82-84 can encode a leucine or no amino acid

<220>
<221>  misc_feature
<222>  (85)..(87)
<223>  nnn at positions 85-87 can encode a leucine or proline

<220>
<221>  misc_feature
<222>  (88)..(90)
<223>  nnn at positions 88-90 can encode a hydrophobic amino acid

<220>
<221>  misc_feature
<222>  (91)..(93)
<223>  nnn at positions 91-93 can encode an isoleucine or valine

<220>
<221>  misc_feature
<222>  (103)..(105)
<223>  nnn at positions 103-105 can encode a hydrophobic amino acid

<220>
<221>  misc_feature
<222>  (112)..(114)
<223>  nnn at positions 112-114 can encode an isoleucine or valine

<220>
<221>  misc_feature
<222>  (121)..(123)
<223>  nnn at positions 121-123 can encode an isoleucine or leucine

<220>
<221>  misc_feature
<222>  (133)..(135)
<223>  nnn at positions 133-135 can encode a proline or leucine

<220>
<221>  misc_feature
<222>  (142)..(144)
<223>  nnn at positions 142-144 can encode an isoleucine or 
       phenylalanine

<400>  9
atggcannnn nnnnntatga ctatnnnnnn nnncccatgn nnnnnnnnnn nnnnnnngac     60

nnngataagn nntgtaagnn nnnnnnnnnn nnnggaattc tgnnnctcct gnnnatcgtg    120

nnnctggggg tgnnnttgat tnnn                                           144


<210>  10
<211>  290
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of mTG reporter construct

<400>  10

Met Ala Ser Thr Ser Tyr Asp Tyr Cys Arg Val Pro Met Glu Asp Gly 
1               5                   10                  15      


Asp Lys Arg Cys Lys Leu Leu Leu Gly Ile Gly Ile Leu Val Leu Leu 
            20                  25                  30          


Ile Ile Val Ile Leu Gly Val Pro Leu Ile Ile Phe Thr Ile Lys Ala 
        35                  40                  45              


Asn Ser Gly Gly Val Ser Lys Gly Ala Glu Leu Phe Thr Gly Ile Val 
    50                  55                  60                  


Pro Ile Leu Ile Glu Leu Asn Gly Asp Val Asn Gly His Lys Phe Ser 
65                  70                  75                  80  


Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
                85                  90                  95      


Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu 
            100                 105                 110         


Val Thr Thr Leu Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 
        115                 120                 125             


His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 
    130                 135                 140                 


Ile Gln Glu Arg Thr Ile Phe Phe Glu Asp Asp Gly Asn Tyr Lys Ser 
145                 150                 155                 160 


Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 
                165                 170                 175     


Leu Thr Gly Thr Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly Asn Lys 
            180                 185                 190         


Met Glu Tyr Asn Tyr Asn Ala His Asn Val Tyr Ile Met Thr Asp Lys 
        195                 200                 205             


Ala Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu 
    210                 215                 220                 


Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 
225                 230                 235                 240 


Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 
                245                 250                 255     


Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Ile Tyr 
            260                 265                 270         


Phe Gly Phe Val Thr Ala Ala Ala Ile Thr His Gly Met Asp Glu Leu 
        275                 280                 285             


Tyr Lys 
    290 


<210>  11
<211>  50
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of human Tetherin "stalk" peptide

<400>  11

Met Ala Ser Thr Ser Tyr Asp Tyr Cys Arg Val Pro Met Glu Asp Gly 
1               5                   10                  15      


Asp Lys Arg Cys Lys Leu Leu Leu Gly Ile Gly Ile Leu Val Leu Leu 
            20                  25                  30          


Ile Ile Val Ile Leu Gly Val Pro Leu Ile Ile Phe Thr Ile Lys Ala 
        35                  40                  45              


Asn Ser 
    50  


<210>  12
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Consensus Tetherin transmembrane sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  X at position 1 can be leucine or no amino acid

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  X at position 2 can be leucine or no amino acid

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  X at position 3 can be leucine or proline

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  X at position 4 can be any hydrophobic residue

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  X at position 5 can be a isoleucine or valine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  X at position 9 can be any hydrophobic residue

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  X at position 12 can be an isoleucine or valine

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  X at position 15 can be an isoleucine or leucine

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  X at position 19 can be a proline or leucine

<400>  12

Xaa Xaa Xaa Xaa Xaa Gly Ile Leu Xaa Leu Leu Xaa Ile Val Xaa Leu 
1               5                   10                  15      


Gly Val Xaa Leu Ile 
            20      


<210>  13
<211>  55
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Consensus amino acid sequence of Tetherin "stalk" peptide


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  X at position 3 is serine or proline

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  X at position 4 is threonine or isoleucine

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  X at position 5 is threonine or leucine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  X at position 9 is cysteine or arginine

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  X at position 10 is a postively charged residue

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  X at position 11 is valine or methionine

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  X at position 14 is aspartic acid or no residue

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  X at position 15 is aspartic acid or no residue

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  X at position 16 is isoleucine or no residue

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  X at position 17 is cysteine, tryptophan, or no residue

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  X at position 18 is lysine or no residue

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  X at position 19 is a charged residue

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  X at position 21 is a glycine or arginine

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  X at position 24 is an arginine or cysteine

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  X at position 27 is a leucine or no residue

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  X at position 28 is a leucine or no residue

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  X at position 29 is a leucine or proline

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  X at position 30 is a hydrophobic residue

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  X at position 31 is an isoleucine or valine

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  X at position 35 is a hydrophic residue

<220>
<221>  misc_feature
<222>  (38)..(38)
<223>  X at position 38 is an isoleucine or valine

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  X at position 41 is an isoleucine or leucine

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  X at position 45 is a proline or leucine

<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  X at position 48 is an isoleucine or phenylalanine

<220>
<221>  misc_feature
<222>  (50)..(50)
<223>  X at position 50 is an isoleucine or threonine

<400>  13

Met Ala Xaa Xaa Xaa Tyr Asp Tyr Xaa Xaa Xaa Pro Met Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Asp Xaa Asp Lys Xaa Cys Lys Xaa Xaa Xaa Xaa Xaa Gly 
            20                  25                  30          


Ile Leu Xaa Leu Leu Xaa Ile Val Xaa Leu Gly Val Xaa Leu Ile Xaa 
        35                  40                  45              


Phe Xaa Ile Lys Ala Asn Ser 
    50                  55  


