                         SEQUENCE LISTING

<110>  Codexis, Inc.
       VOLADRI, Rama
       GOODING, Owen
       JENNE, Stephan
       MUNDORFF, Emily
 
<120>  KETOREDUCTASES AND USES THEREOF

<130>  CX2-023 (376247-019USP1)

<150>  61/092,238
<151>  2008-08-27

<160>  57    

<170>  PatentIn version 3.5

<210>  1
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon Optimized L. brevis Sequence

<400>  1
atgtctaacc gtctggatgg caaagtagcc atcattaccg gcgggactct gggtatcggt       60

ttggcaatcg ccacgaaatt tgtagaggag ggtgcgaaag taatgattac tggtcgtcac      120

tccgatgtag gtgaaaaggc cgccaaatca gtaggcactc cggatcagat tcagtttttt      180

cagcacgatt catccgatga agatggctgg acgaaactgt tcgacgccac cgagaaagca      240

ttcggcccgg ttagcacctt agtgaacaat gcagggattg cagttaacaa aagcgttgaa      300

gaaactacca cggccgaatg gcgtaaactg ctggccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttgaggggtt cgtaggcgat ccgagcctgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctgccg      600

ggtgctgagg aagcgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catatatctg tgtgtacctg gcatctaatg aatcgaaatt tgcgacgggt      720

tccgaatttg tggtcgacgg cgggtatacc gcacagtaat ga                         762


<210>  2
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Translation of Codon Optimized L. brevis Sequence

<400>  2

Met Ser Asn Arg Leu Asp Gly Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Thr Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Met Ile Thr Gly Arg His Ser Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Val Gly Thr Pro Asp Gln Ile Gln Phe Phe Gln His Asp Ser 
    50                  55                  60                  


Ser Asp Glu Asp Gly Trp Thr Lys Leu Phe Asp Ala Thr Glu Lys Ala 
65                  70                  75                  80  


Phe Gly Pro Val Ser Thr Leu Val Asn Asn Ala Gly Ile Ala Val Asn 
                85                  90                  95      


Lys Ser Val Glu Glu Thr Thr Thr Ala Glu Trp Arg Lys Leu Leu Ala 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Glu Gly Phe Val Gly Asp Pro Ser Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Pro Gly Ala Glu Glu Ala Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Tyr Ile Cys Val Tyr Leu Ala Ser Asn Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ser Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  3
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon Optimized L. kefir Sequence

<400>  3
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttgaggggtt cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  4
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Translation of Codon Optimized L. kefir Sequence

<400>  4

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Glu Gly Phe Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  5
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  5
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgccac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagttgccaa aagcgttgaa      300

gacactacca tggaggaatg gcgtaaactg ctgtccgtta atctggatag cgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tcagtgggtt cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgttgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  6
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  6

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ala 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Met Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Ser Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Phe Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Val Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  7
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  7
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgccac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatag tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tcagtgggtt cgtaggcgat ccgacactgg gggcatacac tgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacgtgg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  8
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  8

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Ser Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Phe Val Gly Asp Pro Thr Leu Gly Ala Tyr Thr Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Val Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  9
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  9
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgccac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacca tggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tcagtgggtt cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtattgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  10
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  10

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Met Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Phe Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ile Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  11
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  11
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgccac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacca tggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tcagtgggtt cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgttgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  12
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  12

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Met Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Phe Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Val Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  13
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  13
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgccac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acggaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagttgccaa aagcgttgaa      300

gacactacca tggaggaatg gcgtaaactg ctgtccgtta atctggatag cgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tcagtgggtt cgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgccgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  14
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  14

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Glu Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ala 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Met Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Ser Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Phe Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  15
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  15
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgcttcgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagttaagaa aagcgtcgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttgaggggtt cgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgagatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  16
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  16

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Lys 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Glu Gly Phe Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Arg Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  17
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  17
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgcttcgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagttcagaa aagcgtcgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttgaggggtt cgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgagatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  18
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  18

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Gln 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Glu Gly Phe Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Arg Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  19
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  19
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgcttcgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagttcgtaa aagcgtcgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttgaggggtt cgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgagatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  20
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  20

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Arg 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Glu Gly Phe Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Arg Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  21
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  21
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgcttcgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgtcgaa      300

gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttgaggggtt cgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgagatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  22
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  22

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Glu Gly Phe Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Arg Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  23
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  23
atgaccgatc gtctgaaggg caaagtagct atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac gggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcagcccgg ttacgaccgt cgtgaacaat gcagggattg cagttgcgaa aagcgttgaa      300

gacactacta cggaggaatg gcgtaagctg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tttccgggtt cgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggttc gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  24
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  24

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Ser Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ala 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Phe Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  25
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  25
atgaccgatc gtctgaaggg caaagtagct atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac gggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcagcccgg ttacgaccgt cgtgaacaat gcagggattg cagttgcgaa aagcgttgaa      300

gacactacta cggaggaatg gcgtaagctg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tttccgggtt cgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggttc gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacgtcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  26
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  26

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Ser Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ala 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Phe Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Val Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  27
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  27
atgaccgatc gtctgaaggg caaagtagct atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac gggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcagcccgg ttacgaccgt cgtgaacaat gcagggattg cagttgcgaa aagcgttgaa      300

gacactacta cggaggaatg gcgtaagctg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tttccgggtt cgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggttc gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacgtcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  28
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  28

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Ser Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ala 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Phe Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Val Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  29
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  29
atgaccgatc gtctgaaggg caaagtagct atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac gggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcagcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacta cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta ttgaggggtt cgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggttc gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacgtcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  30
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  30

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Ser Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Glu Gly Phe Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Val Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  31
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  31
atgaccgatc gtctgaaggg caaagtagct atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac gggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcagcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacta cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tttccgggat ggtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggttc gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacgtcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  32
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  32

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Ser Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Met Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Val Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  33
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  33
atgaccgatc gtctgaaggg caaagtagct atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac gggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcagcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacta cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tttccgggct ggtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggttc gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacgtcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  34
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  34

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Ser Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Leu Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Val Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  35
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  35
atgaccgatc gtctgaaggg caaagtagct atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac gggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcagcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacta cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tttccggggt ggtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggttc gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacgtcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  36
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  36

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Ser Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Val Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Val Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  37
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  37
atgaccgatc gtctgaaggg caaagtagct atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac gggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcagcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacta cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tttccggggt tgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggttc gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa gggctacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacgtcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  38
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  38

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Ser Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Val Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Gly Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Val Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  39
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  39
atgaccgatc gtctgaaggg caaagtagct atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac gggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcagcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacta cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tttccgggtg tgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggttc gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacgtcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  40
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  40

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Ser Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Cys Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Val Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  41
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  41
atgaccgatc gtctgaaggg caaagtagct atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac gggtcgtcac      120

gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcagcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacta cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tttccgggtt cgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggttc gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacgtcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  42
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  42

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Ser Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Phe Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Val Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  43
<211>  759
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  43
atgaccgatc gtctgaaggg caaagtagct atcgtaaccg gcgggactct gggtatcggt       60

ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac gggtcgtcac      120

gcggatgtag gtgaaagggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc      180

cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca      240

ttcagcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa      300

gacactacta cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc      360

ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat      420

atgagcagta tttccgggtt cgtaggcgat ccgacgcagg gggcatacaa cgcttccaag      480

ggggcggttc gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat      540

gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa      600

ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg      660

aatgacgtcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt      720

gcagaatttg tggtcgacgg cgggtatacc gcacagtga                             759


<210>  44
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of L. kefir

<400>  44

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Arg Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Ser Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Ser Gly Phe Val Gly Asp Pro Thr Gln Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Val Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  45
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered Ketoreductase Sequence Formula with L. kefir backbone 
       Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (46)..(46)
<223>  Xaa is a basic residue

<220>
<221>  MISC_FEATURE
<222>  (72)..(72)
<223>  Xaa is an acidic residue

<220>
<221>  MISC_FEATURE
<222>  (82)..(82)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is an aliphatic, basic, non-polar, or polar residue

<220>
<221>  MISC_FEATURE
<222>  (104)..(104)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (117)..(117)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is an aliphatic, cysteine, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (177)..(177)
<223>  Xaa is an acidic or basic residue

<220>
<221>  MISC_FEATURE
<222>  (178)..(178)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (194)..(194)
<223>  Xaa is an aliphatic, constrained or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  Xaa is an aliphatic, basic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is an aliphatic, basic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (223)..(223)
<223>  Xaa is an aliphatic or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (236)..(236)
<223>  Xaa is basic residue

<400>  45

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Xaa Ala Ala 
        35                  40                  45              


Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Ala Gly Trp Thr Xaa Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Xaa Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Xaa 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Xaa Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Xaa Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Xaa Gly Xaa Val Gly Asp Pro Thr Xaa Gly Ala Tyr Xaa Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Xaa Xaa Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Xaa Xaa Val Asp Asp Leu Glu Gly Xaa Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Xaa Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Xaa Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  46
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered Ketoreductase Sequence Formula with L. brevis backbone
       Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (46)..(46)
<223>  Xaa is a basic residue

<220>
<221>  MISC_FEATURE
<222>  (72)..(72)
<223>  Xaa is an acidic residue

<220>
<221>  MISC_FEATURE
<222>  (82)..(82)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is an aliphatic, basic, non-polar, or polar residue

<220>
<221>  MISC_FEATURE
<222>  (104)..(104)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (117)..(117)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is an aliphatic, cysteine, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (177)..(177)
<223>  Xaa is an acidic or basic residue

<220>
<221>  MISC_FEATURE
<222>  (178)..(178)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (194)..(194)
<223>  Xaa is an aliphatic, constrained or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  Xaa is an aliphatic, basic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is an aliphatic, basic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (223)..(223)
<223>  Xaa is an aliphatic or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (236)..(236)
<223>  Xaa is basic residue

<400>  46

Met Ser Asn Arg Leu Asp Gly Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Thr Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Met Ile Thr Gly Arg His Ser Asp Val Gly Glu Xaa Ala Ala 
        35                  40                  45              


Lys Ser Val Gly Thr Pro Asp Gln Ile Gln Phe Phe Gln His Asp Ser 
    50                  55                  60                  


Ser Asp Glu Asp Gly Trp Thr Xaa Leu Phe Asp Ala Thr Glu Lys Ala 
65                  70                  75                  80  


Phe Xaa Pro Val Ser Thr Leu Val Asn Asn Ala Gly Ile Ala Val Xaa 
                85                  90                  95      


Lys Ser Val Glu Glu Thr Thr Xaa Ala Glu Trp Arg Lys Leu Leu Ala 
            100                 105                 110         


Val Asn Leu Asp Xaa Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Xaa Gly Xaa Val Gly Asp Pro Ser Xaa Gly Ala Tyr Xaa Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Xaa Xaa Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Xaa Xaa Val Asp Asp Leu Pro Gly Xaa Glu Glu Ala Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Xaa Ala 
    210                 215                 220                 


Tyr Ile Cys Val Tyr Leu Ala Ser Asn Glu Ser Xaa Phe Ala Thr Gly 
225                 230                 235                 240 


Ser Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  47
<211>  759
<212>  DNA
<213>  Lactobacillus minor

<400>  47
atgaccgatc ggttgaaggg gaaagtagca attgtaactg gcggtacctt gggaattggc       60

ttggcaatcg ctgataagtt tgttgaagaa ggcgcaaagg ttgttattac cggccgtcac      120

gctgatgtag gtgaaaaagc tgccagatca atcggcggca cagacgttat ccgttttgtc      180

caacacgatg cttctgatga aaccggctgg actaagttgt ttgatacgac tgaagaagca      240

tttggcccag ttaccacggt tgtcaacaat gccggaattg cggtcagcaa gagtgttgaa      300

gataccacaa ctgaagaatg gcgcaagctg ctctcagtta acttggatgg tgtcttcttc      360

ggtacccgtc ttggaatcca acgtatgaag aataaaggac tcggagcatc aatcatcaat      420

atgtcatcta tcgaaggttt tgttggtgat ccagctctgg gtgcatacaa cgcttcaaaa      480

ggtgctgtca gaattatgtc taaatcagct gccttggatt gcgctttgaa ggactacgat      540

gttcgggtta acactgttca tccaggttat atcaagacac cattggttga cgatcttgaa      600

ggggcagaag aaatgatgtc acagcggacc aagacaccaa tgggtcatat cggtgaacct      660

aacgatatcg cttggatctg tgtttacctg gcatctgacg aatctaaatt tgccactggt      720

gcagaattcg ttgtcgacgg agggtacacc gcccaatag                             759


<210>  48
<211>  252
<212>  PRT
<213>  Lactobacillus minor

<400>  48

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              


Arg Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Thr Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Glu Gly Phe Val Gly Asp Pro Ala Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


<210>  49
<211>  1056
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized ADH from T. brockii

<400>  49
atgaaaggct tcgccatgct gagcatcggc aaagtgggtt ggattgaaaa agaaaaaccg       60

gcgccaggcc cgttcgatgc aattgtgcgc cctctggcag tagcgccgtg taccagcgat      120

attcatactg tgtttgaagg tgccattggc gagcgtcaca atatgattct gggccatgaa      180

gccgttggtg aagttgttga ggttggcagc gaagtgaagg atttcaaacc gggcgatcgc      240

gttgtcgttc cagcgattac cccggattgg cgcaccagcg aagtccagcg cggctaccat      300

cagcactctg gcggcatgct ggccggctgg aaattcagca atgtaaagga tggtgtgttc      360

ggtgaatttt ttcacgttaa cgacgcagac atgaatctgg cgcacctgcc gaaagaaatc      420

ccgctggaag cagcggttat gattccggat atgatgacca cgggttttca cggcgcagag      480

ctggcggaca ttgaactggg cgctacggta gccgtactgg gcatcggtcc ggtgggcctg      540

atggcagttg caggcgctaa gctgcgcggc gcaggtcgta ttattgccgt tggttctcgc      600

ccggtgtgtg tggacgccgc taagtattat ggtgcaacgg acattgtcaa ttacaaggac      660

ggcccaattg aatctcagat catgaacctg acggaaggta aaggcgttga cgccgcgatt      720

atcgctggcg gcaacgccga catcatggcg accgcagtta aaatcgtcaa gccaggtggt      780

actattgcta acgtcaacta cttcggcgaa ggtgaggtcc tgcctgtccc acgtctggaa      840

tggggttgcg gtatggcaca taaaaccatt aaaggtggcc tgtgcccagg cggccgtctg      900

cgtatggaac gcctgatcga tctggtcttc tacaaacgcg tggatcctag caaactggtg      960

actcacgttt tccgcggctt tgataacatc gaaaaagctt ttatgctgat gaaagataaa     1020

ccgaaagatc tgattaaacc ggttgtcatc ctggct                               1056


<210>  50
<211>  352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADH from T. brockii

<400>  50

Met Lys Gly Phe Ala Met Leu Ser Ile Gly Lys Val Gly Trp Ile Glu 
1               5                   10                  15      


Lys Glu Lys Pro Ala Pro Gly Pro Phe Asp Ala Ile Val Arg Pro Leu 
            20                  25                  30          


Ala Val Ala Pro Cys Thr Ser Asp Ile His Thr Val Phe Glu Gly Ala 
        35                  40                  45              


Ile Gly Glu Arg His Asn Met Ile Leu Gly His Glu Ala Val Gly Glu 
    50                  55                  60                  


Val Val Glu Val Gly Ser Glu Val Lys Asp Phe Lys Pro Gly Asp Arg 
65                  70                  75                  80  


Val Val Val Pro Ala Ile Thr Pro Asp Trp Arg Thr Ser Glu Val Gln 
                85                  90                  95      


Arg Gly Tyr His Gln His Ser Gly Gly Met Leu Ala Gly Trp Lys Phe 
            100                 105                 110         


Ser Asn Val Lys Asp Gly Val Phe Gly Glu Phe Phe His Val Asn Asp 
        115                 120                 125             


Ala Asp Met Asn Leu Ala His Leu Pro Lys Glu Ile Pro Leu Glu Ala 
    130                 135                 140                 


Ala Val Met Ile Pro Asp Met Met Thr Thr Gly Phe His Gly Ala Glu 
145                 150                 155                 160 


Leu Ala Asp Ile Glu Leu Gly Ala Thr Val Ala Val Leu Gly Ile Gly 
                165                 170                 175     


Pro Val Gly Leu Met Ala Val Ala Gly Ala Lys Leu Arg Gly Ala Gly 
            180                 185                 190         


Arg Ile Ile Ala Val Gly Ser Arg Pro Val Cys Val Asp Ala Ala Lys 
        195                 200                 205             


Tyr Tyr Gly Ala Thr Asp Ile Val Asn Tyr Lys Asp Gly Pro Ile Glu 
    210                 215                 220                 


Ser Gln Ile Met Asn Leu Thr Glu Gly Lys Gly Val Asp Ala Ala Ile 
225                 230                 235                 240 


Ile Ala Gly Gly Asn Ala Asp Ile Met Ala Thr Ala Val Lys Ile Val 
                245                 250                 255     


Lys Pro Gly Gly Thr Ile Ala Asn Val Asn Tyr Phe Gly Glu Gly Glu 
            260                 265                 270         


Val Leu Pro Val Pro Arg Leu Glu Trp Gly Cys Gly Met Ala His Lys 
        275                 280                 285             


Thr Ile Lys Gly Gly Leu Cys Pro Gly Gly Arg Leu Arg Met Glu Arg 
    290                 295                 300                 


Leu Ile Asp Leu Val Phe Tyr Lys Arg Val Asp Pro Ser Lys Leu Val 
305                 310                 315                 320 


Thr His Val Phe Arg Gly Phe Asp Asn Ile Glu Lys Ala Phe Met Leu 
                325                 330                 335     


Met Lys Asp Lys Pro Lys Asp Leu Ile Lys Pro Val Val Ile Leu Ala 
            340                 345                 350         


<210>  51
<211>  1125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized ADH from Horse Liver, E isozyme

<400>  51
atgagcacag cagggaaagt aattaaatgc aaagcagctg tgctgtggga ggaaaagaaa       60

ccattttcca ttgaggaggt ggaggtcgca cctccgaagg cccatgaagt ccgtattaag      120

atggtggcca cagggatctg tcgctcagat gaccacgtgg ttagtgggac cctggtcaca      180

cctctgcctg tgatcgcagg ccatgaggca gctggcattg tggagagcat cggggaaggc      240

gtcactacag tacgtccagg tgataaagtc atcccactgt ttactcctca gtgtgggaaa      300

tgccgtgttt gtaaacaccc tgaaggcaac ttctgcttga aaaatgatct gagcatgcct      360

cgtgggacca tgcaggatgg taccagccgt ttcacctgcc gtgggaagcc tatccaccac      420

ttcctgggca ccagcacctt ctcccagtac accgtggtgg acgagatctc agtggccaag      480

atcgatgcgg cctcaccgct ggagaaagtc tgtctgattg gctgtgggtt ttctactggt      540

tatgggtctg cagtcaaggt tgccaaggtc acccagggct ccacctgtgc cgtgtttggc      600

ctgggggggg tgggcctgtc tgttatcatg ggctgtaaag cagccggggc ggcccgtatc      660

attggggtgg acatcaacaa agacaagttt gcaaaggcca aagaagtggg tgccactgag      720

tgtgtcaacc ctcaggacta caagaaacct atccaggagg tgctgacaga aatgagcaat      780

gggggcgtgg atttttcatt tgaagtcatt ggtcgtctgg acaccatggt gactgccttg      840

tcatgctgtc aagaagcata tggtgtgagc gtcatcgtag gggtacctcc tgattcccaa      900

aatctgtcta tgaatcctat gttgctgctg agtgggcgta cctggaaagg ggctattttt      960

ggtggtttta agagtaaaga ttctgtccct aaactggtgg ccgattttat ggctaaaaag     1020

tttgcactgg atcctttaat cacccatgtt ttaccttttg aaaaaattaa tgaagggttt     1080

gacctgctgc gctctgggga gagtatccgt accatcctga cgttt                     1125


<210>  52
<211>  375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADH from Horse Liver, E isozyme

<400>  52

Met Ser Thr Ala Gly Lys Val Ile Lys Cys Lys Ala Ala Val Leu Trp 
1               5                   10                  15      


Glu Glu Lys Lys Pro Phe Ser Ile Glu Glu Val Glu Val Ala Pro Pro 
            20                  25                  30          


Lys Ala His Glu Val Arg Ile Lys Met Val Ala Thr Gly Ile Cys Arg 
        35                  40                  45              


Ser Asp Asp His Val Val Ser Gly Thr Leu Val Thr Pro Leu Pro Val 
    50                  55                  60                  


Ile Ala Gly His Glu Ala Ala Gly Ile Val Glu Ser Ile Gly Glu Gly 
65                  70                  75                  80  


Val Thr Thr Val Arg Pro Gly Asp Lys Val Ile Pro Leu Phe Thr Pro 
                85                  90                  95      


Gln Cys Gly Lys Cys Arg Val Cys Lys His Pro Glu Gly Asn Phe Cys 
            100                 105                 110         


Leu Lys Asn Asp Leu Ser Met Pro Arg Gly Thr Met Gln Asp Gly Thr 
        115                 120                 125             


Ser Arg Phe Thr Cys Arg Gly Lys Pro Ile His His Phe Leu Gly Thr 
    130                 135                 140                 


Ser Thr Phe Ser Gln Tyr Thr Val Val Asp Glu Ile Ser Val Ala Lys 
145                 150                 155                 160 


Ile Asp Ala Ala Ser Pro Leu Glu Lys Val Cys Leu Ile Gly Cys Gly 
                165                 170                 175     


Phe Ser Thr Gly Tyr Gly Ser Ala Val Lys Val Ala Lys Val Thr Gln 
            180                 185                 190         


Gly Ser Thr Cys Ala Val Phe Gly Leu Gly Gly Val Gly Leu Ser Val 
        195                 200                 205             


Ile Met Gly Cys Lys Ala Ala Gly Ala Ala Arg Ile Ile Gly Val Asp 
    210                 215                 220                 


Ile Asn Lys Asp Lys Phe Ala Lys Ala Lys Glu Val Gly Ala Thr Glu 
225                 230                 235                 240 


Cys Val Asn Pro Gln Asp Tyr Lys Lys Pro Ile Gln Glu Val Leu Thr 
                245                 250                 255     


Glu Met Ser Asn Gly Gly Val Asp Phe Ser Phe Glu Val Ile Gly Arg 
            260                 265                 270         


Leu Asp Thr Met Val Thr Ala Leu Ser Cys Cys Gln Glu Ala Tyr Gly 
        275                 280                 285             


Val Ser Val Ile Val Gly Val Pro Pro Asp Ser Gln Asn Leu Ser Met 
    290                 295                 300                 


Asn Pro Met Leu Leu Leu Ser Gly Arg Thr Trp Lys Gly Ala Ile Phe 
305                 310                 315                 320 


Gly Gly Phe Lys Ser Lys Asp Ser Val Pro Lys Leu Val Ala Asp Phe 
                325                 330                 335     


Met Ala Lys Lys Phe Ala Leu Asp Pro Leu Ile Thr His Val Leu Pro 
            340                 345                 350         


Phe Glu Lys Ile Asn Glu Gly Phe Asp Leu Leu Arg Ser Gly Glu Ser 
        355                 360                 365             


Ile Arg Thr Ile Leu Thr Phe 
    370                 375 


<210>  53
<211>  1122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized ADH from Horse Liver, S isozyme

<400>  53
atgagcacag cagggaaagt aattaaatgc aaagcagctg tgctgtggga gcaaaagaaa       60

ccattttcca ttgaggaggt ggaggtcgca cctccgaagg cccatgaagt ccgtattaag      120

atggtggccg cagggatctg tcgctcagat gaccacgtgg ttagtgggac cctggtcgca      180

cctctgcctg tgatcgcagg ccatgaggca gctggcattg tggagagcat cggggaaggc      240

gtcactacag tacgtccagg tgataaagtc atcccactgt ttattcctca gtgtgggaaa      300

tgcagtgttt gtaaacaccc tgaaggcaac ctgtgcttga aaaatctgag catgcctcgt      360

gggaccatgc aggatggtac cagccgtttc acctgccgtg ggaagcctat ccaccacttc      420

ctgggcacca gcaccttctc ccagtacacc gtggtggacg agatctcagt ggccaagatc      480

gatgcggcct caccgctgga gaaagtctgt ctggttggct gtgggttttc tactggttat      540

gggtctgcag tcaaggttgc caaggtcacc cagggctcca cctgtgccgt gtttggcctg      600

gggggggtgg gcctgtctgt tatcatgggc tgtaaagcag ccggggcggc ccgtatcatt      660

ggggtggaca tcaacaaaga caagtttgca aaggccaaag aagtgggtgc cactgagtgt      720

gtcaaccctc aggactacaa gaaacctatc caggaggtgc tgacagaaat gagcaatggg      780

ggcgtggatt tttcatttga agtcattggt cgtctggaca ccatggtggc tgccttgtca      840

tgctgtcaag aagcatatgg tgtgagcgtc atcgtagggg tacctcctga ttcccaaaat      900

ctgtctatga atcctatgtt gctgctgagt gggcgtacct ggaaaggggc tatttttggt      960

ggttttaaga gtaaagattc tgtccctaaa ctggtggccg attttatggc taaaaagttt     1020

gcactggatc ctttaatcac ccatgtttta ccttttgaaa aaattaatga agggtttgac     1080

ctgctgcgct ctgggaagag tatccgtacc atcctgacgt tt                        1122


<210>  54
<211>  374
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADH from Horse Liver, S isozyme

<400>  54

Met Ser Thr Ala Gly Lys Val Ile Lys Cys Lys Ala Ala Val Leu Trp 
1               5                   10                  15      


Glu Gln Lys Lys Pro Phe Ser Ile Glu Glu Val Glu Val Ala Pro Pro 
            20                  25                  30          


Lys Ala His Glu Val Arg Ile Lys Met Val Ala Ala Gly Ile Cys Arg 
        35                  40                  45              


Ser Asp Asp His Val Val Ser Gly Thr Leu Val Ala Pro Leu Pro Val 
    50                  55                  60                  


Ile Ala Gly His Glu Ala Ala Gly Ile Val Glu Ser Ile Gly Glu Gly 
65                  70                  75                  80  


Val Thr Thr Val Arg Pro Gly Asp Lys Val Ile Pro Leu Phe Ile Pro 
                85                  90                  95      


Gln Cys Gly Lys Cys Ser Val Cys Lys His Pro Glu Gly Asn Leu Cys 
            100                 105                 110         


Leu Lys Asn Leu Ser Met Pro Arg Gly Thr Met Gln Asp Gly Thr Ser 
        115                 120                 125             


Arg Phe Thr Cys Arg Gly Lys Pro Ile His His Phe Leu Gly Thr Ser 
    130                 135                 140                 


Thr Phe Ser Gln Tyr Thr Val Val Asp Glu Ile Ser Val Ala Lys Ile 
145                 150                 155                 160 


Asp Ala Ala Ser Pro Leu Glu Lys Val Cys Leu Val Gly Cys Gly Phe 
                165                 170                 175     


Ser Thr Gly Tyr Gly Ser Ala Val Lys Val Ala Lys Val Thr Gln Gly 
            180                 185                 190         


Ser Thr Cys Ala Val Phe Gly Leu Gly Gly Val Gly Leu Ser Val Ile 
        195                 200                 205             


Met Gly Cys Lys Ala Ala Gly Ala Ala Arg Ile Ile Gly Val Asp Ile 
    210                 215                 220                 


Asn Lys Asp Lys Phe Ala Lys Ala Lys Glu Val Gly Ala Thr Glu Cys 
225                 230                 235                 240 


Val Asn Pro Gln Asp Tyr Lys Lys Pro Ile Gln Glu Val Leu Thr Glu 
                245                 250                 255     


Met Ser Asn Gly Gly Val Asp Phe Ser Phe Glu Val Ile Gly Arg Leu 
            260                 265                 270         


Asp Thr Met Val Ala Ala Leu Ser Cys Cys Gln Glu Ala Tyr Gly Val 
        275                 280                 285             


Ser Val Ile Val Gly Val Pro Pro Asp Ser Gln Asn Leu Ser Met Asn 
    290                 295                 300                 


Pro Met Leu Leu Leu Ser Gly Arg Thr Trp Lys Gly Ala Ile Phe Gly 
305                 310                 315                 320 


Gly Phe Lys Ser Lys Asp Ser Val Pro Lys Leu Val Ala Asp Phe Met 
                325                 330                 335     


Ala Lys Lys Phe Ala Leu Asp Pro Leu Ile Thr His Val Leu Pro Phe 
            340                 345                 350         


Glu Lys Ile Asn Glu Gly Phe Asp Leu Leu Arg Ser Gly Lys Ser Ile 
        355                 360                 365             


Arg Thr Ile Leu Thr Phe 
    370                 


<210>  55
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized ADH from O. oeni

<400>  55
atggtcgatc gcctgaaagg taaggtttca attgtgacgg ggggcaccct gggtatcggc       60

ttgtcgattg tcgacctgta cttgaaagaa ggcgccaaag tggtgttcac tggccgtcgc      120

cagaaaattg gggaggaggc attcaaacac ttgtctaacc cgaaaaacgc aaagttcgtg      180

gttcacgatg ctagcaacga agaaggttgg aaaaaactgt ttgccgatgt tattgccgaa      240

ttcggcaaag tggatattct ggtcaataac gcaggcattg gtgtaccggg cgatgtcgag      300

cacacggatt atgcgcaatg gcgtcaaact atggcggtga acctggacgg cgtatacttc      360

ggtactcatt atggtgtaat caacatgaaa caccctgtct ccggtgacgc ctctatcatt      420

aatatgtcct cgattgaagg tttggtaggt gatccaaatc tgttcgccta caacgcaact      480

aaaggcgctc tgcgcatcat gaccaaaagc gccgcgattt actgcgcgca gaacgattac      540

aacctgcgca ttaataccat ccacccgggt tatatcaaaa ctccgttagt agaagacatg      600

gatgggtttg agaaagcgat gtccgaacgc acgaaaaccc ctatgggcca cattggcgat      660

ccggaggaca tcggttggct ggcagtgtat ctgggttcag aagaagcaaa atttgccacg      720

ggcagcgaat tcacagtcga cggtggctac accgcacagt aa                         762


<210>  56
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADH from O. oeni

<400>  56

Met Val Asp Arg Leu Lys Gly Lys Val Ser Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ser Ile Val Asp Leu Tyr Leu Lys Glu Gly Ala 
            20                  25                  30          


Lys Val Val Phe Thr Gly Arg Arg Gln Lys Ile Gly Glu Glu Ala Phe 
        35                  40                  45              


Lys His Leu Ser Asn Pro Lys Asn Ala Lys Phe Val Val His Asp Ala 
    50                  55                  60                  


Ser Asn Glu Glu Gly Trp Lys Lys Leu Phe Ala Asp Val Ile Ala Glu 
65                  70                  75                  80  


Phe Gly Lys Val Asp Ile Leu Val Asn Asn Ala Gly Ile Gly Val Pro 
                85                  90                  95      


Gly Asp Val Glu His Thr Asp Tyr Ala Gln Trp Arg Gln Thr Met Ala 
            100                 105                 110         


Val Asn Leu Asp Gly Val Tyr Phe Gly Thr His Tyr Gly Val Ile Asn 
        115                 120                 125             


Met Lys His Pro Val Ser Gly Asp Ala Ser Ile Ile Asn Met Ser Ser 
    130                 135                 140                 


Ile Glu Gly Leu Val Gly Asp Pro Asn Leu Phe Ala Tyr Asn Ala Thr 
145                 150                 155                 160 


Lys Gly Ala Leu Arg Ile Met Thr Lys Ser Ala Ala Ile Tyr Cys Ala 
                165                 170                 175     


Gln Asn Asp Tyr Asn Leu Arg Ile Asn Thr Ile His Pro Gly Tyr Ile 
            180                 185                 190         


Lys Thr Pro Leu Val Glu Asp Met Asp Gly Phe Glu Lys Ala Met Ser 
        195                 200                 205             


Glu Arg Thr Lys Thr Pro Met Gly His Ile Gly Asp Pro Glu Asp Ile 
    210                 215                 220                 


Gly Trp Leu Ala Val Tyr Leu Gly Ser Glu Glu Ala Lys Phe Ala Thr 
225                 230                 235                 240 


Gly Ser Glu Phe Thr Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250             


<210>  57
<211>  252
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Engineered Ketoreductase Sequence Formula with L. minor backbone 
       Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (46)..(46)
<223>  Xaa is a basic residue

<220>
<221>  MISC_FEATURE
<222>  (72)..(72)
<223>  Xaa is an acidic residue

<220>
<221>  MISC_FEATURE
<222>  (82)..(82)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is an aliphatic, basic, non-polar, or polar residue

<220>
<221>  MISC_FEATURE
<222>  (104)..(104)
<223>  Xaa is a non-polar or polar residue

<220>
<221>  MISC_FEATURE
<222>  (117)..(117)
<223>  Xaa is a polar or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (145)..(145)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is an aliphatic, cysteine, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is a polar residue

<220>
<221>  MISC_FEATURE
<222>  (177)..(177)
<223>  Xaa is an acidic or basic residue

<220>
<221>  MISC_FEATURE
<222>  (178)..(178)
<223>  Xaa is a non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (194)..(194)
<223>  Xaa is an aliphatic, constrained or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (195)..(195)
<223>  Xaa is an aliphatic, basic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is an aliphatic, basic, or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (223)..(223)
<223>  Xaa is an aliphatic or non-polar residue

<220>
<221>  MISC_FEATURE
<222>  (236)..(236)
<223>  Xaa is basic residue

<400>  57

Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      


Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          


Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Xaa Ala Ala 
        35                  40                  45              


Arg Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  


Ser Asp Glu Thr Gly Trp Thr Xaa Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  


Phe Xaa Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Xaa 
                85                  90                  95      


Lys Ser Val Glu Asp Thr Thr Xaa Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         


Val Asn Leu Asp Xaa Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             


Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 


Xaa Gly Xaa Val Gly Asp Pro Ala Xaa Gly Ala Tyr Xaa Ala Ser Lys 
145                 150                 155                 160 


Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     


Xaa Xaa Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         


Thr Xaa Xaa Val Asp Asp Leu Glu Gly Xaa Glu Glu Met Met Ser Gln 
        195                 200                 205             


Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Xaa Ala 
    210                 215                 220                 


Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Xaa Phe Ala Thr Gly 
225                 230                 235                 240 


Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln 
                245                 250         


