                         SEQUENCE LISTING

<110>  Advanced Technologies (Cambridge) Limited
 
<120>  Transgenic plants

<130>  AXH/57177PCT1

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  2093
<212>  DNA
<213>  Arabidopsis thaliana

<400>  1
tcgagacccg attgttattt ttagactgag acaaaaaagt agaatcgttg attgttaaaa       60

tttaaaatta gtttcattac gtttcgataa aaaaatgatt agtttatcat agcttaatta      120

tagcattgat ttctaaattt gttttttgac cacccttttt tctctctttg gtgttttctt      180

aacattagaa gaacccataa caatgtacgt tcaaattaat taaaaacaat atttccaagt      240

tttatatacg aaacttgttt tttttaatga aaacagttga atagttgatt atgaattagt      300

tagatcaata ctcaatatat gatcaatgat gtatatatat gaactcagtt gttatacaag      360

aaatgaaaat gctatttaaa tacagatcat gaagtgttaa aaagtgtcag aatatgacat      420

gaagcgtttt gtcctaccgg gtattcgagt tataggtttg gatctctcaa gaatattttg      480

ggccatacta gttatatttg ggcttaagcg ttttgcaaag agacgaggaa gaaagattgg      540

gtcaagttaa caaaacagag acactcgtat tagttggtac tttggtagca agtcgattta      600

tttgccagta aaaacttggt acacaactga caactcgtat cgttattagt ttgtacttgg      660

tacctttggt tcaagaaaaa gttgatatag ttaaatcagt tgtgttcatg aggtgattgt      720

gatttaattt gttgactagg gcgattcctt cacatcacaa taacaaagtt ttatagattt      780

tttttttata acatttttgc cacgcttcgt aaagtttggt atttacaccg catttttccc      840

tgtacaagaa ttcatatatt atttatttat atactccagt tgacaattat aagtttataa      900

cgtttttaca attatttaaa taccatgtga agatccaaga atatgtctta cttcttcttt      960

gtgtaagaaa actaactata tcactataat aaaataattc taatcattat atttgtaaat     1020

atgcagttat ttgtcaattt tgaatttagt attttagacg ttatcacttc agccaaatat     1080

gatttggatt taagtccaaa atgcaatttc gtacgtatcc ctcttgtcgt ctaatgatta     1140

tttcaatatt tcttatatta tccctaacta cagagctaca tttatattgt attctaatga     1200

cagggaaacc ttcatagaga ttcagataga tgaaattggt gggaaacatc attgaacagg     1260

aaacttttag caaatcatat cgatttatct acaaaagaat acgtagcgta atgaagtcca     1320

cttgttgtga atgactatga tttgatcaaa ttagttaatt ttgtcgaatc atttttcttt     1380

ttgatttgat taagctttta acttgcacga atggttctct tgtgaataaa cagaatcttt     1440

gaattcaaac tatttgatta gtgaaaagac aaaagaagat tccttgtttt tatgtgatta     1500

gtgattttga tgcatgaaag gtacctacgt actacaagaa aaataaacat gtacgtaact     1560

acgtatcagc atgtaaaagt atttttttcc aaataattta tactcatgat agattttttt     1620

tttttgaaat gtcaattaaa aatgctttct taaatattaa ttttaattaa ttaaataagg     1680

aaatatattt atgcaaaaca tcatcaacac atatccaact tcgaaaatct ctatagtaca     1740

caagtagaga aattaaattt tactagatac aaacttccta atcatcaaat ataaatgttt     1800

acaaaactaa ttaaacccac cactaaaatt aactaaaaat ccgagcaaag tgagtgaaca     1860

agacttgatt tcaggttgat gtaggactaa aatgactacg tatcaaacat caacgatcat     1920

ttagttatgt atgaatgaat gtagtcatta cttgtaaaac aaaaatgctt tgatttggat     1980

caatcacttc atgtgaacat tagcaattac atcaacctta ttttcactat aaaaccccat     2040

ctcagtaccc ttctgaagta atcaaattaa gagcaaaagt catttaactt agg            2093


<210>  2
<211>  2751
<212>  DNA
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) single mutant (Q443A); R = A or G

<400>  2
atggcgactc tgaagccgtc atttactgtt tctccgccga atagtaatcc gattagattt       60

ggaagttttc cgccgcaatg ctttctccgt gttccgaaac cgcggcgact tatattgcct      120

aggtttcgga agacgactgg tggtggcggc ggcttgattc gatgtgagct tccagatttt      180

catctatcag caacagcaac tactgtatca ggtgtatcga cggtgaattt agtggatcaa      240

gttcagattc ctaaaggtga aatgtggagt gttcacaagt ttggtgggac ttgtgtggga      300

aactctcaga ggatcagaaa tgtagcagag gttataatca atgataattc cgaaagaaaa      360

cttgtggttg tctcggcgat gtcgaaggtt acggacatga tgtatgactt aatccgcaag      420

gcacaatcac gagatgattc ttatttatcc gcgttggaag ctgtcttgga aaagcatcgt      480

ttaacagctc gtgaccttct cgatggagat gatctcgcta gtttcttgtc acatttgcat      540

aatgatatta gtaatcttaa agcaatgctt cgtgctatat acatagctgg ccatgcatca      600

gagtcgtttt cagattttgt tgcaggacat ggggagcttt ggtctgctca gatgctatca      660

tatgttgtca gaaagactgg gcttgagtgc aagtggatgg atactagaga cgtgctcatt      720

gttaatccca ccagctctaa tcaggttgat cctgattttg gtgaatctga gaagagactc      780

gataaatggt tctccttaaa tccgtcgaaa attattattg cgactgggtt tattgctagc      840

actccgcaaa atattccaac aactttgaaa agagatggga gtgatttctc agcagctatt      900

atgggtgctt tattgagagc tcgtcaagta accatttgga cagatgttga tggtgtatac      960

agtgcggatc ctcgtaaagt taatgaggca gtgatactcc agacactttc ttatcaagag     1020

gcctgggaaa tgtcttattt tggagcaaat gtgttacatc ctcgcaccat cattcctgtg     1080

atgcgatata atattccgat tgtgattaga aatattttca atctctctgc accgggaaca     1140

ataatctgtc aacctcctga agatgattat gaccttaaac tgacaactcc tgtcaaaggg     1200

tttgcaacta ttgacaattt ggccctcata aatgttgaag gtactggaat ggctggtgta     1260

cccggtactg caagtgacat ttttggctgt gtaaaagatg ttggagctaa tgtgattatg     1320

atatcagctg ctagcagtga gcattctgtg tgctttgctg tgcctgagaa ggaagtaaac     1380

gcagtctctg aggcattgcg gtcgagattt agtgaagctt tacaagcggg acgtctttct     1440

cagattgagg tgataccaaa ctgtagcatc ttagctgcag tcggccagaa aatggctagt     1500

acacctggag ttagttgtac acttttcagt gctttggcga aggctaatat taatgtccga     1560

gctatatctc arggttgttc tgagtacaat gttactgtcg ttattaaacg tgaagatagc     1620

gttaaggcgt taagagctgt acactcgagg tttttcttgt caagaacaac attagcaatg     1680

ggaatcgtag gaccgggctt gattggtgca acattacttg accagctgcg ggatcaggct     1740

gctgttctca aacaagaatt taacattgat ctgcgtgttt tgggaatcac tggttcaaag     1800

aagatgttat tgagtgacat tggtattgat ttgtcgagat ggagagaact tctaaacgag     1860

aagggaacag aggcggattt ggataaattc actcaacaag tgcatggaaa tcattttatc     1920

cccaactctg tagtggttga ttgtacagca gactctgcta ttgcaagccg ttactatgat     1980

tggttacgaa agggaattca tgtcattacc ccaaataaaa aggctaactc aggtcccctc     2040

gatcagtact tgaaactgag agatcttcaa aggaaatcct acactcatta cttctacgaa     2100

gccactgttg gagctggtct tccaattatc agcactttac gtggtctcct tgagacagga     2160

gataagatac tacgcataga gggcatttgc agtggaactt tgagttatct attcaacaat     2220

tttgttggag atcgaagttt cagcgaggtt gtcactgaag caaagaacgc aggtttcact     2280

gagcctgatc caagagatga tttatctgga actgatgttg caaggaaggt gattatcctc     2340

gctcgagaat ctggactgaa attggacctc gctgatctcc ccattagaag tctcgtacca     2400

gaacctctaa aaggatgcac ttctgttgaa gaattcatgg agaaactccc acagtacgat     2460

ggagacctag caaaagaaag gctagatgct gaaaactctg gggaagttct gagatatgtt     2520

ggagtggtgg acgctgttaa ccaaaaggga acagttgaac ttcgaagata caagaaagaa     2580

catccatttg cgcagctcgc aggttcagac aacataatag ccttcacaac gacaaggtac     2640

aaggatcatc cacttatagt ccgaggacct ggagctggtg ctcaagtcac ggccggtggt     2700

atattcagcg acatactaag gcttgcatct tatctcggtg caccgtctta a              2751


<210>  3
<211>  2751
<212>  DNA
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) single mutant (Q524A); R = A or G

<400>  3
atggcgactc tgaagccgtc atttactgtt tctccgccga atagtaatcc gattagattt       60

ggaagttttc cgccgcaatg ctttctccgt gttccgaaac cgcggcgact tatattgcct      120

aggtttcgga agacgactgg tggtggcggc ggcttgattc gatgtgagct tccagatttt      180

catctatcag caacagcaac tactgtatca ggtgtatcga cggtgaattt agtggatcaa      240

gttcagattc ctaaaggtga aatgtggagt gttcacaagt ttggtgggac ttgtgtggga      300

aactctcaga ggatcagaaa tgtagcagag gttataatca atgataattc cgaaagaaaa      360

cttgtggttg tctcggcgat gtcgaaggtt acggacatga tgtatgactt aatccgcaag      420

gcacaatcac gagatgattc ttatttatcc gcgttggaag ctgtcttgga aaagcatcgt      480

ttaacagctc gtgaccttct cgatggagat gatctcgcta gtttcttgtc acatttgcat      540

aatgatatta gtaatcttaa agcaatgctt cgtgctatat acatagctgg ccatgcatca      600

gagtcgtttt cagattttgt tgcaggacat ggggagcttt ggtctgctca gatgctatca      660

tatgttgtca gaaagactgg gcttgagtgc aagtggatgg atactagaga cgtgctcatt      720

gttaatccca ccagctctaa tcaggttgat cctgattttg gtgaatctga gaagagactc      780

gataaatggt tctccttaaa tccgtcgaaa attattattg cgactgggtt tattgctagc      840

actccgcaaa atattccaac aactttgaaa agagatggga gtgatttctc agcagctatt      900

atgggtgctt tattgagagc tcgtcaagta accatttgga cagatgttga tggtgtatac      960

agtgcggatc ctcgtaaagt taatgaggca gtgatactcc agacactttc ttatcaagag     1020

gcctgggaaa tgtcttattt tggagcaaat gtgttacatc ctcgcaccat cattcctgtg     1080

atgcgatata atattccgat tgtgattaga aatattttca atctctctgc accgggaaca     1140

ataatctgtc aacctcctga agatgattat gaccttaaac tgacaactcc tgtcaaaggg     1200

tttgcaacta ttgacaattt ggccctcata aatgttgaag gtactggaat ggctggtgta     1260

cccggtactg caagtgacat ttttggctgt gtaaaagatg ttggagctaa tgtgattatg     1320

atatcacarg ctagcagtga gcattctgtg tgctttgctg tgcctgagaa ggaagtaaac     1380

gcagtctctg aggcattgcg gtcgagattt agtgaagctt tacaagcggg acgtctttct     1440

cagattgagg tgataccaaa ctgtagcatc ttagctgcag tcggccagaa aatggctagt     1500

acacctggag ttagttgtac acttttcagt gctttggcga aggctaatat taatgtccga     1560

gctatatctg ctggttgttc tgagtacaat gttactgtcg ttattaaacg tgaagatagc     1620

gttaaggcgt taagagctgt acactcgagg tttttcttgt caagaacaac attagcaatg     1680

ggaatcgtag gaccgggctt gattggtgca acattacttg accagctgcg ggatcaggct     1740

gctgttctca aacaagaatt taacattgat ctgcgtgttt tgggaatcac tggttcaaag     1800

aagatgttat tgagtgacat tggtattgat ttgtcgagat ggagagaact tctaaacgag     1860

aagggaacag aggcggattt ggataaattc actcaacaag tgcatggaaa tcattttatc     1920

cccaactctg tagtggttga ttgtacagca gactctgcta ttgcaagccg ttactatgat     1980

tggttacgaa agggaattca tgtcattacc ccaaataaaa aggctaactc aggtcccctc     2040

gatcagtact tgaaactgag agatcttcaa aggaaatcct acactcatta cttctacgaa     2100

gccactgttg gagctggtct tccaattatc agcactttac gtggtctcct tgagacagga     2160

gataagatac tacgcataga gggcatttgc agtggaactt tgagttatct attcaacaat     2220

tttgttggag atcgaagttt cagcgaggtt gtcactgaag caaagaacgc aggtttcact     2280

gagcctgatc caagagatga tttatctgga actgatgttg caaggaaggt gattatcctc     2340

gctcgagaat ctggactgaa attggacctc gctgatctcc ccattagaag tctcgtacca     2400

gaacctctaa aaggatgcac ttctgttgaa gaattcatgg agaaactccc acagtacgat     2460

ggagacctag caaaagaaag gctagatgct gaaaactctg gggaagttct gagatatgtt     2520

ggagtggtgg acgctgttaa ccaaaaggga acagttgaac ttcgaagata caagaaagaa     2580

catccatttg cgcagctcgc aggttcagac aacataatag ccttcacaac gacaaggtac     2640

aaggatcatc cacttatagt ccgaggacct ggagctggtg ctcaagtcac ggccggtggt     2700

atattcagcg acatactaag gcttgcatct tatctcggtg caccgtctta a              2751


<210>  4
<211>  2751
<212>  DNA
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) double mutant (Q443A; Q524A); R = A 
       or G

<400>  4
atggcgactc tgaagccgtc atttactgtt tctccgccga atagtaatcc gattagattt       60

ggaagttttc cgccgcaatg ctttctccgt gttccgaaac cgcggcgact tatattgcct      120

aggtttcgga agacgactgg tggtggcggc ggcttgattc gatgtgagct tccagatttt      180

catctatcag caacagcaac tactgtatca ggtgtatcga cggtgaattt agtggatcaa      240

gttcagattc ctaaaggtga aatgtggagt gttcacaagt ttggtgggac ttgtgtggga      300

aactctcaga ggatcagaaa tgtagcagag gttataatca atgataattc cgaaagaaaa      360

cttgtggttg tctcggcgat gtcgaaggtt acggacatga tgtatgactt aatccgcaag      420

gcacaatcac gagatgattc ttatttatcc gcgttggaag ctgtcttgga aaagcatcgt      480

ttaacagctc gtgaccttct cgatggagat gatctcgcta gtttcttgtc acatttgcat      540

aatgatatta gtaatcttaa agcaatgctt cgtgctatat acatagctgg ccatgcatca      600

gagtcgtttt cagattttgt tgcaggacat ggggagcttt ggtctgctca gatgctatca      660

tatgttgtca gaaagactgg gcttgagtgc aagtggatgg atactagaga cgtgctcatt      720

gttaatccca ccagctctaa tcaggttgat cctgattttg gtgaatctga gaagagactc      780

gataaatggt tctccttaaa tccgtcgaaa attattattg cgactgggtt tattgctagc      840

actccgcaaa atattccaac aactttgaaa agagatggga gtgatttctc agcagctatt      900

atgggtgctt tattgagagc tcgtcaagta accatttgga cagatgttga tggtgtatac      960

agtgcggatc ctcgtaaagt taatgaggca gtgatactcc agacactttc ttatcaagag     1020

gcctgggaaa tgtcttattt tggagcaaat gtgttacatc ctcgcaccat cattcctgtg     1080

atgcgatata atattccgat tgtgattaga aatattttca atctctctgc accgggaaca     1140

ataatctgtc aacctcctga agatgattat gaccttaaac tgacaactcc tgtcaaaggg     1200

tttgcaacta ttgacaattt ggccctcata aatgttgaag gtactggaat ggctggtgta     1260

cccggtactg caagtgacat ttttggctgt gtaaaagatg ttggagctaa tgtgattatg     1320

atatcagctg ctagcagtga gcattctgtg tgctttgctg tgcctgagaa ggaagtaaac     1380

gcagtctctg aggcattgcg gtcgagattt agtgaagctt tacaagcggg acgtctttct     1440

cagattgagg tgataccaaa ctgtagcatc ttagctgcag tcggccagaa aatggctagt     1500

acacctggag ttagttgtac acttttcagt gctttggcga aggctaatat taatgtccga     1560

gctatatctg ctggttgttc tgagtacaat gttactgtcg ttattaaacg tgaagatagc     1620

gttaaggcgt taagagctgt acactcgagg tttttcttgt caagaacaac attagcaatg     1680

ggaatcgtag gaccgggctt gattggtgca acattacttg accagctgcg ggatcaggct     1740

gctgttctca aacaagaatt taacattgat ctgcgtgttt tgggaatcac tggttcaaag     1800

aagatgttat tgagtgacat tggtattgat ttgtcgagat ggagagaact tctaaacgag     1860

aagggaacag aggcggattt ggataaattc actcaacaag tgcatggaaa tcattttatc     1920

cccaactctg tagtggttga ttgtacagca gactctgcta ttgcaagccg ttactatgat     1980

tggttacgaa agggaattca tgtcattacc ccaaataaaa aggctaactc aggtcccctc     2040

gatcagtact tgaaactgag agatcttcaa aggaaatcct acactcatta cttctacgaa     2100

gccactgttg gagctggtct tccaattatc agcactttac gtggtctcct tgagacagga     2160

gataagatac tacgcataga gggcatttgc agtggaactt tgagttatct attcaacaat     2220

tttgttggag atcgaagttt cagcgaggtt gtcactgaag caaagaacgc aggtttcact     2280

gagcctgatc caagagatga tttatctgga actgatgttg caaggaaggt gattatcctc     2340

gctcgagaat ctggactgaa attggacctc gctgatctcc ccattagaag tctcgtacca     2400

gaacctctaa aaggatgcac ttctgttgaa gaattcatgg agaaactccc acagtacgat     2460

ggagacctag caaaagaaag gctagatgct gaaaactctg gggaagttct gagatatgtt     2520

ggagtggtgg acgctgttaa ccaaaaggga acagttgaac ttcgaagata caagaaagaa     2580

catccatttg cgcagctcgc aggttcagac aacataatag ccttcacaac gacaaggtac     2640

aaggatcatc cacttatagt ccgaggacct ggagctggtg ctcaagtcac ggccggtggt     2700

atattcagcg acatactaag gcttgcatct tatctcggtg caccgtctta a              2751


<210>  5
<211>  916
<212>  PRT
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) single mutant (Q443A)

<400>  5

Met Ala Thr Leu Lys Pro Ser Phe Thr Val Ser Pro Pro Asn Ser Asn 
1               5                   10                  15      


Pro Ile Arg Phe Gly Ser Phe Pro Pro Gln Cys Phe Leu Arg Val Pro 
            20                  25                  30          


Lys Pro Arg Arg Leu Ile Leu Pro Arg Phe Arg Lys Thr Thr Gly Gly 
        35                  40                  45              


Gly Gly Gly Leu Ile Arg Cys Glu Leu Pro Asp Phe His Leu Ser Ala 
    50                  55                  60                  


Thr Ala Thr Thr Val Ser Gly Val Ser Thr Val Asn Leu Val Asp Gln 
65                  70                  75                  80  


Val Gln Ile Pro Lys Gly Glu Met Trp Ser Val His Lys Phe Gly Gly 
                85                  90                  95      


Thr Cys Val Gly Asn Ser Gln Arg Ile Arg Asn Val Ala Glu Val Ile 
            100                 105                 110         


Ile Asn Asp Asn Ser Glu Arg Lys Leu Val Val Val Ser Ala Met Ser 
        115                 120                 125             


Lys Val Thr Asp Met Met Tyr Asp Leu Ile Arg Lys Ala Gln Ser Arg 
    130                 135                 140                 


Asp Asp Ser Tyr Leu Ser Ala Leu Glu Ala Val Leu Glu Lys His Arg 
145                 150                 155                 160 


Leu Thr Ala Arg Asp Leu Leu Asp Gly Asp Asp Leu Ala Ser Phe Leu 
                165                 170                 175     


Ser His Leu His Asn Asp Ile Ser Asn Leu Lys Ala Met Leu Arg Ala 
            180                 185                 190         


Ile Tyr Ile Ala Gly His Ala Ser Glu Ser Phe Ser Asp Phe Val Ala 
        195                 200                 205             


Gly His Gly Glu Leu Trp Ser Ala Gln Met Leu Ser Tyr Val Val Arg 
    210                 215                 220                 


Lys Thr Gly Leu Glu Cys Lys Trp Met Asp Thr Arg Asp Val Leu Ile 
225                 230                 235                 240 


Val Asn Pro Thr Ser Ser Asn Gln Val Asp Pro Asp Phe Gly Glu Ser 
                245                 250                 255     


Glu Lys Arg Leu Asp Lys Trp Phe Ser Leu Asn Pro Ser Lys Ile Ile 
            260                 265                 270         


Ile Ala Thr Gly Phe Ile Ala Ser Thr Pro Gln Asn Ile Pro Thr Thr 
        275                 280                 285             


Leu Lys Arg Asp Gly Ser Asp Phe Ser Ala Ala Ile Met Gly Ala Leu 
    290                 295                 300                 


Leu Arg Ala Arg Gln Val Thr Ile Trp Thr Asp Val Asp Gly Val Tyr 
305                 310                 315                 320 


Ser Ala Asp Pro Arg Lys Val Asn Glu Ala Val Ile Leu Gln Thr Leu 
                325                 330                 335     


Ser Tyr Gln Glu Ala Trp Glu Met Ser Tyr Phe Gly Ala Asn Val Leu 
            340                 345                 350         


His Pro Arg Thr Ile Ile Pro Val Met Arg Tyr Asn Ile Pro Ile Val 
        355                 360                 365             


Ile Arg Asn Ile Phe Asn Leu Ser Ala Pro Gly Thr Ile Ile Cys Gln 
    370                 375                 380                 


Pro Pro Glu Asp Asp Tyr Asp Leu Lys Leu Thr Thr Pro Val Lys Gly 
385                 390                 395                 400 


Phe Ala Thr Ile Asp Asn Leu Ala Leu Ile Asn Val Glu Gly Thr Gly 
                405                 410                 415     


Met Ala Gly Val Pro Gly Thr Ala Ser Asp Ile Phe Gly Cys Val Lys 
            420                 425                 430         


Asp Val Gly Ala Asn Val Ile Met Ile Ser Ala Ala Ser Ser Glu His 
        435                 440                 445             


Ser Val Cys Phe Ala Val Pro Glu Lys Glu Val Asn Ala Val Ser Glu 
    450                 455                 460                 


Ala Leu Arg Ser Arg Phe Ser Glu Ala Leu Gln Ala Gly Arg Leu Ser 
465                 470                 475                 480 


Gln Ile Glu Val Ile Pro Asn Cys Ser Ile Leu Ala Ala Val Gly Gln 
                485                 490                 495     


Lys Met Ala Ser Thr Pro Gly Val Ser Cys Thr Leu Phe Ser Ala Leu 
            500                 505                 510         


Ala Lys Ala Asn Ile Asn Val Arg Ala Ile Ser Gln Gly Cys Ser Glu 
        515                 520                 525             


Tyr Asn Val Thr Val Val Ile Lys Arg Glu Asp Ser Val Lys Ala Leu 
    530                 535                 540                 


Arg Ala Val His Ser Arg Phe Phe Leu Ser Arg Thr Thr Leu Ala Met 
545                 550                 555                 560 


Gly Ile Val Gly Pro Gly Leu Ile Gly Ala Thr Leu Leu Asp Gln Leu 
                565                 570                 575     


Arg Asp Gln Ala Ala Val Leu Lys Gln Glu Phe Asn Ile Asp Leu Arg 
            580                 585                 590         


Val Leu Gly Ile Thr Gly Ser Lys Lys Met Leu Leu Ser Asp Ile Gly 
        595                 600                 605             


Ile Asp Leu Ser Arg Trp Arg Glu Leu Leu Asn Glu Lys Gly Thr Glu 
    610                 615                 620                 


Ala Asp Leu Asp Lys Phe Thr Gln Gln Val His Gly Asn His Phe Ile 
625                 630                 635                 640 


Pro Asn Ser Val Val Val Asp Cys Thr Ala Asp Ser Ala Ile Ala Ser 
                645                 650                 655     


Arg Tyr Tyr Asp Trp Leu Arg Lys Gly Ile His Val Ile Thr Pro Asn 
            660                 665                 670         


Lys Lys Ala Asn Ser Gly Pro Leu Asp Gln Tyr Leu Lys Leu Arg Asp 
        675                 680                 685             


Leu Gln Arg Lys Ser Tyr Thr His Tyr Phe Tyr Glu Ala Thr Val Gly 
    690                 695                 700                 


Ala Gly Leu Pro Ile Ile Ser Thr Leu Arg Gly Leu Leu Glu Thr Gly 
705                 710                 715                 720 


Asp Lys Ile Leu Arg Ile Glu Gly Ile Cys Ser Gly Thr Leu Ser Tyr 
                725                 730                 735     


Leu Phe Asn Asn Phe Val Gly Asp Arg Ser Phe Ser Glu Val Val Thr 
            740                 745                 750         


Glu Ala Lys Asn Ala Gly Phe Thr Glu Pro Asp Pro Arg Asp Asp Leu 
        755                 760                 765             


Ser Gly Thr Asp Val Ala Arg Lys Val Ile Ile Leu Ala Arg Glu Ser 
    770                 775                 780                 


Gly Leu Lys Leu Asp Leu Ala Asp Leu Pro Ile Arg Ser Leu Val Pro 
785                 790                 795                 800 


Glu Pro Leu Lys Gly Cys Thr Ser Val Glu Glu Phe Met Glu Lys Leu 
                805                 810                 815     


Pro Gln Tyr Asp Gly Asp Leu Ala Lys Glu Arg Leu Asp Ala Glu Asn 
            820                 825                 830         


Ser Gly Glu Val Leu Arg Tyr Val Gly Val Val Asp Ala Val Asn Gln 
        835                 840                 845             


Lys Gly Thr Val Glu Leu Arg Arg Tyr Lys Lys Glu His Pro Phe Ala 
    850                 855                 860                 


Gln Leu Ala Gly Ser Asp Asn Ile Ile Ala Phe Thr Thr Thr Arg Tyr 
865                 870                 875                 880 


Lys Asp His Pro Leu Ile Val Arg Gly Pro Gly Ala Gly Ala Gln Val 
                885                 890                 895     


Thr Ala Gly Gly Ile Phe Ser Asp Ile Leu Arg Leu Ala Ser Tyr Leu 
            900                 905                 910         


Gly Ala Pro Ser 
        915     


<210>  6
<211>  916
<212>  PRT
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) single mutant (Q524A)

<400>  6

Met Ala Thr Leu Lys Pro Ser Phe Thr Val Ser Pro Pro Asn Ser Asn 
1               5                   10                  15      


Pro Ile Arg Phe Gly Ser Phe Pro Pro Gln Cys Phe Leu Arg Val Pro 
            20                  25                  30          


Lys Pro Arg Arg Leu Ile Leu Pro Arg Phe Arg Lys Thr Thr Gly Gly 
        35                  40                  45              


Gly Gly Gly Leu Ile Arg Cys Glu Leu Pro Asp Phe His Leu Ser Ala 
    50                  55                  60                  


Thr Ala Thr Thr Val Ser Gly Val Ser Thr Val Asn Leu Val Asp Gln 
65                  70                  75                  80  


Val Gln Ile Pro Lys Gly Glu Met Trp Ser Val His Lys Phe Gly Gly 
                85                  90                  95      


Thr Cys Val Gly Asn Ser Gln Arg Ile Arg Asn Val Ala Glu Val Ile 
            100                 105                 110         


Ile Asn Asp Asn Ser Glu Arg Lys Leu Val Val Val Ser Ala Met Ser 
        115                 120                 125             


Lys Val Thr Asp Met Met Tyr Asp Leu Ile Arg Lys Ala Gln Ser Arg 
    130                 135                 140                 


Asp Asp Ser Tyr Leu Ser Ala Leu Glu Ala Val Leu Glu Lys His Arg 
145                 150                 155                 160 


Leu Thr Ala Arg Asp Leu Leu Asp Gly Asp Asp Leu Ala Ser Phe Leu 
                165                 170                 175     


Ser His Leu His Asn Asp Ile Ser Asn Leu Lys Ala Met Leu Arg Ala 
            180                 185                 190         


Ile Tyr Ile Ala Gly His Ala Ser Glu Ser Phe Ser Asp Phe Val Ala 
        195                 200                 205             


Gly His Gly Glu Leu Trp Ser Ala Gln Met Leu Ser Tyr Val Val Arg 
    210                 215                 220                 


Lys Thr Gly Leu Glu Cys Lys Trp Met Asp Thr Arg Asp Val Leu Ile 
225                 230                 235                 240 


Val Asn Pro Thr Ser Ser Asn Gln Val Asp Pro Asp Phe Gly Glu Ser 
                245                 250                 255     


Glu Lys Arg Leu Asp Lys Trp Phe Ser Leu Asn Pro Ser Lys Ile Ile 
            260                 265                 270         


Ile Ala Thr Gly Phe Ile Ala Ser Thr Pro Gln Asn Ile Pro Thr Thr 
        275                 280                 285             


Leu Lys Arg Asp Gly Ser Asp Phe Ser Ala Ala Ile Met Gly Ala Leu 
    290                 295                 300                 


Leu Arg Ala Arg Gln Val Thr Ile Trp Thr Asp Val Asp Gly Val Tyr 
305                 310                 315                 320 


Ser Ala Asp Pro Arg Lys Val Asn Glu Ala Val Ile Leu Gln Thr Leu 
                325                 330                 335     


Ser Tyr Gln Glu Ala Trp Glu Met Ser Tyr Phe Gly Ala Asn Val Leu 
            340                 345                 350         


His Pro Arg Thr Ile Ile Pro Val Met Arg Tyr Asn Ile Pro Ile Val 
        355                 360                 365             


Ile Arg Asn Ile Phe Asn Leu Ser Ala Pro Gly Thr Ile Ile Cys Gln 
    370                 375                 380                 


Pro Pro Glu Asp Asp Tyr Asp Leu Lys Leu Thr Thr Pro Val Lys Gly 
385                 390                 395                 400 


Phe Ala Thr Ile Asp Asn Leu Ala Leu Ile Asn Val Glu Gly Thr Gly 
                405                 410                 415     


Met Ala Gly Val Pro Gly Thr Ala Ser Asp Ile Phe Gly Cys Val Lys 
            420                 425                 430         


Asp Val Gly Ala Asn Val Ile Met Ile Ser Gln Ala Ser Ser Glu His 
        435                 440                 445             


Ser Val Cys Phe Ala Val Pro Glu Lys Glu Val Asn Ala Val Ser Glu 
    450                 455                 460                 


Ala Leu Arg Ser Arg Phe Ser Glu Ala Leu Gln Ala Gly Arg Leu Ser 
465                 470                 475                 480 


Gln Ile Glu Val Ile Pro Asn Cys Ser Ile Leu Ala Ala Val Gly Gln 
                485                 490                 495     


Lys Met Ala Ser Thr Pro Gly Val Ser Cys Thr Leu Phe Ser Ala Leu 
            500                 505                 510         


Ala Lys Ala Asn Ile Asn Val Arg Ala Ile Ser Ala Gly Cys Ser Glu 
        515                 520                 525             


Tyr Asn Val Thr Val Val Ile Lys Arg Glu Asp Ser Val Lys Ala Leu 
    530                 535                 540                 


Arg Ala Val His Ser Arg Phe Phe Leu Ser Arg Thr Thr Leu Ala Met 
545                 550                 555                 560 


Gly Ile Val Gly Pro Gly Leu Ile Gly Ala Thr Leu Leu Asp Gln Leu 
                565                 570                 575     


Arg Asp Gln Ala Ala Val Leu Lys Gln Glu Phe Asn Ile Asp Leu Arg 
            580                 585                 590         


Val Leu Gly Ile Thr Gly Ser Lys Lys Met Leu Leu Ser Asp Ile Gly 
        595                 600                 605             


Ile Asp Leu Ser Arg Trp Arg Glu Leu Leu Asn Glu Lys Gly Thr Glu 
    610                 615                 620                 


Ala Asp Leu Asp Lys Phe Thr Gln Gln Val His Gly Asn His Phe Ile 
625                 630                 635                 640 


Pro Asn Ser Val Val Val Asp Cys Thr Ala Asp Ser Ala Ile Ala Ser 
                645                 650                 655     


Arg Tyr Tyr Asp Trp Leu Arg Lys Gly Ile His Val Ile Thr Pro Asn 
            660                 665                 670         


Lys Lys Ala Asn Ser Gly Pro Leu Asp Gln Tyr Leu Lys Leu Arg Asp 
        675                 680                 685             


Leu Gln Arg Lys Ser Tyr Thr His Tyr Phe Tyr Glu Ala Thr Val Gly 
    690                 695                 700                 


Ala Gly Leu Pro Ile Ile Ser Thr Leu Arg Gly Leu Leu Glu Thr Gly 
705                 710                 715                 720 


Asp Lys Ile Leu Arg Ile Glu Gly Ile Cys Ser Gly Thr Leu Ser Tyr 
                725                 730                 735     


Leu Phe Asn Asn Phe Val Gly Asp Arg Ser Phe Ser Glu Val Val Thr 
            740                 745                 750         


Glu Ala Lys Asn Ala Gly Phe Thr Glu Pro Asp Pro Arg Asp Asp Leu 
        755                 760                 765             


Ser Gly Thr Asp Val Ala Arg Lys Val Ile Ile Leu Ala Arg Glu Ser 
    770                 775                 780                 


Gly Leu Lys Leu Asp Leu Ala Asp Leu Pro Ile Arg Ser Leu Val Pro 
785                 790                 795                 800 


Glu Pro Leu Lys Gly Cys Thr Ser Val Glu Glu Phe Met Glu Lys Leu 
                805                 810                 815     


Pro Gln Tyr Asp Gly Asp Leu Ala Lys Glu Arg Leu Asp Ala Glu Asn 
            820                 825                 830         


Ser Gly Glu Val Leu Arg Tyr Val Gly Val Val Asp Ala Val Asn Gln 
        835                 840                 845             


Lys Gly Thr Val Glu Leu Arg Arg Tyr Lys Lys Glu His Pro Phe Ala 
    850                 855                 860                 


Gln Leu Ala Gly Ser Asp Asn Ile Ile Ala Phe Thr Thr Thr Arg Tyr 
865                 870                 875                 880 


Lys Asp His Pro Leu Ile Val Arg Gly Pro Gly Ala Gly Ala Gln Val 
                885                 890                 895     


Thr Ala Gly Gly Ile Phe Ser Asp Ile Leu Arg Leu Ala Ser Tyr Leu 
            900                 905                 910         


Gly Ala Pro Ser 
        915     


<210>  7
<211>  916
<212>  PRT
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) double mutant (Q443A; Q524A)

<400>  7

Met Ala Thr Leu Lys Pro Ser Phe Thr Val Ser Pro Pro Asn Ser Asn 
1               5                   10                  15      


Pro Ile Arg Phe Gly Ser Phe Pro Pro Gln Cys Phe Leu Arg Val Pro 
            20                  25                  30          


Lys Pro Arg Arg Leu Ile Leu Pro Arg Phe Arg Lys Thr Thr Gly Gly 
        35                  40                  45              


Gly Gly Gly Leu Ile Arg Cys Glu Leu Pro Asp Phe His Leu Ser Ala 
    50                  55                  60                  


Thr Ala Thr Thr Val Ser Gly Val Ser Thr Val Asn Leu Val Asp Gln 
65                  70                  75                  80  


Val Gln Ile Pro Lys Gly Glu Met Trp Ser Val His Lys Phe Gly Gly 
                85                  90                  95      


Thr Cys Val Gly Asn Ser Gln Arg Ile Arg Asn Val Ala Glu Val Ile 
            100                 105                 110         


Ile Asn Asp Asn Ser Glu Arg Lys Leu Val Val Val Ser Ala Met Ser 
        115                 120                 125             


Lys Val Thr Asp Met Met Tyr Asp Leu Ile Arg Lys Ala Gln Ser Arg 
    130                 135                 140                 


Asp Asp Ser Tyr Leu Ser Ala Leu Glu Ala Val Leu Glu Lys His Arg 
145                 150                 155                 160 


Leu Thr Ala Arg Asp Leu Leu Asp Gly Asp Asp Leu Ala Ser Phe Leu 
                165                 170                 175     


Ser His Leu His Asn Asp Ile Ser Asn Leu Lys Ala Met Leu Arg Ala 
            180                 185                 190         


Ile Tyr Ile Ala Gly His Ala Ser Glu Ser Phe Ser Asp Phe Val Ala 
        195                 200                 205             


Gly His Gly Glu Leu Trp Ser Ala Gln Met Leu Ser Tyr Val Val Arg 
    210                 215                 220                 


Lys Thr Gly Leu Glu Cys Lys Trp Met Asp Thr Arg Asp Val Leu Ile 
225                 230                 235                 240 


Val Asn Pro Thr Ser Ser Asn Gln Val Asp Pro Asp Phe Gly Glu Ser 
                245                 250                 255     


Glu Lys Arg Leu Asp Lys Trp Phe Ser Leu Asn Pro Ser Lys Ile Ile 
            260                 265                 270         


Ile Ala Thr Gly Phe Ile Ala Ser Thr Pro Gln Asn Ile Pro Thr Thr 
        275                 280                 285             


Leu Lys Arg Asp Gly Ser Asp Phe Ser Ala Ala Ile Met Gly Ala Leu 
    290                 295                 300                 


Leu Arg Ala Arg Gln Val Thr Ile Trp Thr Asp Val Asp Gly Val Tyr 
305                 310                 315                 320 


Ser Ala Asp Pro Arg Lys Val Asn Glu Ala Val Ile Leu Gln Thr Leu 
                325                 330                 335     


Ser Tyr Gln Glu Ala Trp Glu Met Ser Tyr Phe Gly Ala Asn Val Leu 
            340                 345                 350         


His Pro Arg Thr Ile Ile Pro Val Met Arg Tyr Asn Ile Pro Ile Val 
        355                 360                 365             


Ile Arg Asn Ile Phe Asn Leu Ser Ala Pro Gly Thr Ile Ile Cys Gln 
    370                 375                 380                 


Pro Pro Glu Asp Asp Tyr Asp Leu Lys Leu Thr Thr Pro Val Lys Gly 
385                 390                 395                 400 


Phe Ala Thr Ile Asp Asn Leu Ala Leu Ile Asn Val Glu Gly Thr Gly 
                405                 410                 415     


Met Ala Gly Val Pro Gly Thr Ala Ser Asp Ile Phe Gly Cys Val Lys 
            420                 425                 430         


Asp Val Gly Ala Asn Val Ile Met Ile Ser Ala Ala Ser Ser Glu His 
        435                 440                 445             


Ser Val Cys Phe Ala Val Pro Glu Lys Glu Val Asn Ala Val Ser Glu 
    450                 455                 460                 


Ala Leu Arg Ser Arg Phe Ser Glu Ala Leu Gln Ala Gly Arg Leu Ser 
465                 470                 475                 480 


Gln Ile Glu Val Ile Pro Asn Cys Ser Ile Leu Ala Ala Val Gly Gln 
                485                 490                 495     


Lys Met Ala Ser Thr Pro Gly Val Ser Cys Thr Leu Phe Ser Ala Leu 
            500                 505                 510         


Ala Lys Ala Asn Ile Asn Val Arg Ala Ile Ser Ala Gly Cys Ser Glu 
        515                 520                 525             


Tyr Asn Val Thr Val Val Ile Lys Arg Glu Asp Ser Val Lys Ala Leu 
    530                 535                 540                 


Arg Ala Val His Ser Arg Phe Phe Leu Ser Arg Thr Thr Leu Ala Met 
545                 550                 555                 560 


Gly Ile Val Gly Pro Gly Leu Ile Gly Ala Thr Leu Leu Asp Gln Leu 
                565                 570                 575     


Arg Asp Gln Ala Ala Val Leu Lys Gln Glu Phe Asn Ile Asp Leu Arg 
            580                 585                 590         


Val Leu Gly Ile Thr Gly Ser Lys Lys Met Leu Leu Ser Asp Ile Gly 
        595                 600                 605             


Ile Asp Leu Ser Arg Trp Arg Glu Leu Leu Asn Glu Lys Gly Thr Glu 
    610                 615                 620                 


Ala Asp Leu Asp Lys Phe Thr Gln Gln Val His Gly Asn His Phe Ile 
625                 630                 635                 640 


Pro Asn Ser Val Val Val Asp Cys Thr Ala Asp Ser Ala Ile Ala Ser 
                645                 650                 655     


Arg Tyr Tyr Asp Trp Leu Arg Lys Gly Ile His Val Ile Thr Pro Asn 
            660                 665                 670         


Lys Lys Ala Asn Ser Gly Pro Leu Asp Gln Tyr Leu Lys Leu Arg Asp 
        675                 680                 685             


Leu Gln Arg Lys Ser Tyr Thr His Tyr Phe Tyr Glu Ala Thr Val Gly 
    690                 695                 700                 


Ala Gly Leu Pro Ile Ile Ser Thr Leu Arg Gly Leu Leu Glu Thr Gly 
705                 710                 715                 720 


Asp Lys Ile Leu Arg Ile Glu Gly Ile Cys Ser Gly Thr Leu Ser Tyr 
                725                 730                 735     


Leu Phe Asn Asn Phe Val Gly Asp Arg Ser Phe Ser Glu Val Val Thr 
            740                 745                 750         


Glu Ala Lys Asn Ala Gly Phe Thr Glu Pro Asp Pro Arg Asp Asp Leu 
        755                 760                 765             


Ser Gly Thr Asp Val Ala Arg Lys Val Ile Ile Leu Ala Arg Glu Ser 
    770                 775                 780                 


Gly Leu Lys Leu Asp Leu Ala Asp Leu Pro Ile Arg Ser Leu Val Pro 
785                 790                 795                 800 


Glu Pro Leu Lys Gly Cys Thr Ser Val Glu Glu Phe Met Glu Lys Leu 
                805                 810                 815     


Pro Gln Tyr Asp Gly Asp Leu Ala Lys Glu Arg Leu Asp Ala Glu Asn 
            820                 825                 830         


Ser Gly Glu Val Leu Arg Tyr Val Gly Val Val Asp Ala Val Asn Gln 
        835                 840                 845             


Lys Gly Thr Val Glu Leu Arg Arg Tyr Lys Lys Glu His Pro Phe Ala 
    850                 855                 860                 


Gln Leu Ala Gly Ser Asp Asn Ile Ile Ala Phe Thr Thr Thr Arg Tyr 
865                 870                 875                 880 


Lys Asp His Pro Leu Ile Val Arg Gly Pro Gly Ala Gly Ala Gln Val 
                885                 890                 895     


Thr Ala Gly Gly Ile Phe Ser Asp Ile Leu Arg Leu Ala Ser Tyr Leu 
            900                 905                 910         


Gly Ala Pro Ser 
        915     


<210>  8
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) wild type PCR primers (forward)

<400>  8
atggcgactc tgaagccgtc atttac                                            26


<210>  9
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) wild type PCR primers (reverse)

<400>  9
ttaagacggt gcaccgagat aagatgc                                           27


<210>  10
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) mutant PCR primers (Q443A) (forward)

<400>  10
gtgattatga tatcagctgc tagcagtgag cattctg                                37


<210>  11
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) mutant PCR primers (Q443A) (reverse)

<400>  11
cagaatgctc actgctagca gctgatatca taattcac                               38


<210>  12
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) mutant PCR primers (Q524A) (forward)

<400>  12
gtccgagcta tatctgctgg ttgttctgag tacaatg                                37


<210>  13
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Aspartate kinase (At4g19710) mutant PCR primers (Q524A) (reverse)

<400>  13
cattgatctc agaacaacca gcagatatag ctcggac                                37


