                         SEQUENCE LISTING

<110>  Wyeth
       Geerligs, Harmen Jacob
       Meinders, Cindy Aleida Maria
       Boelm, Geert Jan
       Stuurman, Bastiana Geertruida Elisabeth
 
<120>  INFECTIOUS BRONCHITIS VACCINES DERIVED FROM IB-QX-LIKE STRAINS

<130>  AM103152

<150>  61/087228
<151>  2008-08-08

<160>  8     

<170>  PatentIn version 3.4

<210>  1
<211>  1657
<212>  DNA
<213>  Infectious Bronchitis Virus QX

<400>  1
atgttgggga agtcactgtt tttagtgacc attttgtgtg cactatgtag tgcaaatttg     60

ttcgattctg ctaataatta tgtgtactac taccaaagtg cctttaggcc tccaaatgga    120

tggcatttgc aagggggtgc ttatgcagta gtgaattcca ctaattatag taataatgca    180

ggttctgcac ctcagtgcac tgttggtgtt attaaggacg tctataatca aagtgcggct    240

tctatagcta tgacagcacc tcttcagggt atggcttggt ctaagtcaca attttgtagt    300

gcacactgta acttttctga aattacagtt tttgtcacac attgttatag tagtggtagc    360

gggtcttgtc ctataacagg catgattcca cgtgatcata ttcgtatttc tgcaatgaaa    420

aatggttctt tattttataa tttaacagtt agcgtatcta aataccctaa ttttaaatct    480

tttcaatgtg ttaacaactt cacatctgtt tatttaaatg gtgatcttgt ttttacttcc    540

aacaaaacta ctgatgttac gtcagcaggt gtgtatttta aagcaggtgg acctgtaaat    600

tataatatta tgaaagaatt taaggttctt gcttactttg ttaatggtac agcacaagat    660

gtaattttgt gcgataattc ccccaagggt ttgctagcct gtcaatataa cactggcaat    720

ttttcagatg gcttttatcc ttttactaat agtactttag ttagggaaaa gttcattgtc    780

tatcgcgaaa gtagtgttaa tactactctg gcgttaacta atttcacttt tactaatgta    840

agtaatgcac agcctaatag tggtggtgtt aatacttttc atttatatca aacacaaaca    900

gctcagagtg gttattataa ttttaatttg tcatttctga gtcagtttgt gtataaggca    960

agtgatttta tgtatgggtc ttaccaccct agttgttctt ttagaccaga aaccattaat   1020

agtggtttgt ggtttaattc cttgtcagtt tctcttactt atggacccct acagggaggg   1080

tgtaagcaat ctgtttttag tggtaaggca acgtgttgtt atgcctactc ttataatggc   1140

ccaagggcat gtaaaggtgt ttattcaggt gaattaagca tgaattttga atgtggattg   1200

ctggtttatg ttactaagag tcatggctct cgtatacaga ctagaacgga gcccttagta   1260

ttaacgcaac acaattataa taatattact ttagataagt gtgttgctta taatatatat   1320

ggcagagtag gccaaggttt tattactaat gtgactgatt ctgctgctaa ttttagttat   1380

ttagcagatg gtgggttagc tattttagat acgtcgggtg ccatagatgt ttttgttgta   1440

aagggcagct atggtcttaa ttattacaag gttaatcctt gtgaagatgt taaccaacag   1500

tttgtagtgt ctggtggcaa tatagttggc attcttactt ctagaaatga aacaggttct   1560

gaacaggttg agaaccagtt ttatgttaag ttaaccaata gctcacatcg tcgcaggcgt   1620

tctattggcc aaaacgtaac aacttgccct tatgtta                            1657


<210>  2
<211>  601
<212>  PRT
<213>  Infectious Bronchitis Virus QX Like L1148

<400>  2

Met Leu Val Lys Ser Leu Phe Leu Val Thr Ile Leu Cys Ala Leu Cys 
1               5                   10                  15      


Ser Ala Asn Leu Phe Asp Ser Asp Asn Asn Tyr Val Tyr Tyr Tyr Gln 
            20                  25                  30          


Ser Ala Phe Arg Pro Pro Asn Gly Trp His Leu Gln Gly Gly Ala Tyr 
        35                  40                  45              


Ala Val Val Asn Ser Thr Asn Tyr Thr Asn Asn Ala Gly Ser Ala His 
    50                  55                  60                  


Glu Cys Thr Val Gly Val Ile Lys Asp Val Tyr Asn Gln Ser Val Ala 
65                  70                  75                  80  


Ser Ile Ala Met Thr Ala Pro Leu Gln Gly Met Ala Trp Ser Lys Ser 
                85                  90                  95      


Gln Phe Cys Ser Ala His Cys Asn Phe Ser Glu Ile Thr Val Phe Val 
            100                 105                 110         


Thr His Cys Tyr Ser Ser Gly Ser Gly Ser Cys Pro Ile Thr Gly Met 
        115                 120                 125             


Ile Pro Arg Asp His Ile Arg Ile Ser Ala Met Lys Asn Gly Ser Leu 
    130                 135                 140                 


Phe Tyr Asn Leu Thr Val Ser Val Ser Lys Tyr Pro Asn Phe Lys Ser 
145                 150                 155                 160 


Phe Gln Cys Val Asn Asn Phe Thr Ser Val Tyr Leu Asn Gly Asp Leu 
                165                 170                 175     


Val Phe Thr Ser Asn Lys Thr Thr Asp Val Thr Ser Ala Gly Val Tyr 
            180                 185                 190         


Phe Lys Ala Gly Gly Pro Val Asn Tyr Ser Ile Met Lys Glu Phe Lys 
        195                 200                 205             


Val Leu Ala Tyr Phe Val Asn Gly Thr Ala Gln Asp Val Val Leu Cys 
    210                 215                 220                 


Asp Asn Ser Pro Lys Gly Leu Leu Ala Cys Gln Tyr Asn Thr Gly Asn 
225                 230                 235                 240 


Phe Ser Asp Gly Phe Tyr Pro Phe Thr Asn Ser Thr Leu Val Arg Glu 
                245                 250                 255     


Lys Phe Ile Val Tyr Arg Glu Ser Ser Val Asn Thr Thr Leu Ala Leu 
            260                 265                 270         


Thr Asn Phe Thr Phe Thr Asn Val Ser Asn Ala Gln Pro Asn Ser Gly 
        275                 280                 285             


Gly Val Asn Thr Phe His Leu Tyr Gln Thr Gln Thr Ala Gln Ser Gly 
    290                 295                 300                 


Tyr Tyr Asn Phe Asn Leu Ser Phe Leu Ser Gln Phe Val Tyr Lys Ala 
305                 310                 315                 320 


Ser Asp Phe Met Tyr Gly Ser Tyr His Pro Ser Cys Ser Phe Arg Pro 
                325                 330                 335     


Glu Thr Ile Asn Ser Gly Leu Trp Phe Asn Ser Leu Ser Val Ser Leu 
            340                 345                 350         


Thr Tyr Gly Pro Leu Gln Gly Gly Cys Lys Gln Ser Val Phe Ser Gly 
        355                 360                 365             


Lys Ala Thr Cys Cys Tyr Ala Tyr Ser Tyr Lys Gly Pro Met Ala Cys 
    370                 375                 380                 


Lys Gly Val Tyr Ser Gly Glu Leu Ser Thr Asn Phe Glu Cys Gly Leu 
385                 390                 395                 400 


Leu Val Tyr Val Thr Lys Ser Asp Gly Ser Arg Ile Gln Thr Arg Thr 
                405                 410                 415     


Glu Pro Leu Val Leu Thr Gln Tyr Asn Tyr Asn Asn Ile Thr Leu Asp 
            420                 425                 430         


Lys Cys Val Ala Tyr Asn Ile Tyr Gly Arg Val Gly Gln Gly Phe Ile 
        435                 440                 445             


Thr Asn Val Thr Asp Ser Ala Ala Asn Phe Ser Tyr Leu Ala Asp Gly 
    450                 455                 460                 


Gly Leu Ala Ile Leu Asp Thr Ser Gly Ala Ile Asp Val Phe Val Val 
465                 470                 475                 480 


Gln Gly Ile Tyr Gly Leu Asn Tyr Tyr Lys Val Asn Pro Cys Glu Asp 
                485                 490                 495     


Val Asn Gln Gln Phe Val Val Ser Gly Gly Asn Ile Val Gly Ile Leu 
            500                 505                 510         


Thr Ser Arg Asn Glu Thr Gly Ser Glu Gln Val Glu Asn Gln Phe Tyr 
        515                 520                 525             


Val Lys Leu Thr Asn Ser Ser His Arg Arg Arg Arg Ser Ile Gly Gln 
    530                 535                 540                 


Asn Val Thr Ser Cys Pro Tyr Val Ser Tyr Gly Arg Phe Cys Ile Glu 
545                 550                 555                 560 


Pro Asp Gly Ser Leu Lys Met Ile Val Pro Glu Glu Leu Lys Gln Phe 
                565                 570                 575     


Val Ala Pro Leu Leu Asn Ile Thr Glu Ser Val Leu Ile Pro Asn Ser 
            580                 585                 590         


Phe Asn Leu Thr Val Pro Pro Arg Asn 
        595                 600     


<210>  3
<211>  567
<212>  PRT
<213>  Infectious Bronchitis Virus QX LIke L1449-2

<400>  3

Met Leu Val Lys Ser Leu Phe Leu Val Thr Ile Leu Cys Ala Leu Cys 
1               5                   10                  15      


Ser Ala Asn Leu Phe Asp Ser Asp Asn Asn Tyr Val Tyr Tyr Tyr Gln 
            20                  25                  30          


Ser Ala Phe Arg Pro Pro Asn Gly Trp His Leu Gln Gly Gly Ala Tyr 
        35                  40                  45              


Ala Val Val Asn Ser Thr Asn Tyr Thr Asn Asn Ala Gly Ser Ala His 
    50                  55                  60                  


Gly Cys Thr Val Gly Val Ile Lys Asp Val Tyr Asn Gln Ser Val Ala 
65                  70                  75                  80  


Ser Ile Ala Met Thr Ala Pro Leu Gln Gly Met Ala Trp Ser Lys Ser 
                85                  90                  95      


Gln Phe Cys Ser Ala His Cys Asn Phe Ser Glu Ile Thr Val Phe Val 
            100                 105                 110         


Thr His Cys Tyr Ser Ser Gly Ser Gly Ser Cys Pro Ile Thr Gly Met 
        115                 120                 125             


Ile Pro Arg Asp His Ile Arg Ile Ser Ala Met Lys Asn Gly Ser Leu 
    130                 135                 140                 


Phe Tyr Asn Leu Thr Val Ser Val Ser Lys Tyr Pro Asn Phe Lys Ser 
145                 150                 155                 160 


Phe Gln Cys Val Asn Asn Phe Thr Ser Val Tyr Leu Asn Gly Asp Leu 
                165                 170                 175     


Val Phe Thr Ser Asn Lys Thr Thr Asp Val Thr Ser Ala Gly Val Tyr 
            180                 185                 190         


Phe Lys Ala Gly Gly Pro Val Asn Tyr Ser Ile Met Lys Glu Phe Lys 
        195                 200                 205             


Val Leu Ala Tyr Phe Val Asn Gly Thr Ala Gln Asp Val Ile Leu Cys 
    210                 215                 220                 


Asp Asn Ser Pro Lys Gly Leu Leu Ala Cys Gln Tyr Asn Thr Gly Asn 
225                 230                 235                 240 


Phe Ser Asp Gly Phe Tyr Pro Phe Thr Asn Ser Thr Leu Val Arg Glu 
                245                 250                 255     


Lys Phe Ile Val Tyr Arg Glu Ser Ser Val Asn Thr Thr Leu Ala Leu 
            260                 265                 270         


Thr Asn Phe Thr Phe Thr Asn Val Ser Asn Ala Gln Pro Asn Ser Gly 
        275                 280                 285             


Gly Val Asn Thr Phe His Leu Tyr Gln Thr Gln Thr Ala Gln Ser Gly 
    290                 295                 300                 


Tyr Tyr Asn Phe Asn Leu Ser Phe Leu Ser Gln Phe Val Tyr Lys Ala 
305                 310                 315                 320 


Ser Asp Phe Met Tyr Gly Ser Tyr His Pro Ser Cys Ser Phe Arg Pro 
                325                 330                 335     


Glu Thr Ile Asn Ser Gly Leu Trp Phe Asn Ser Leu Ser Val Ser Leu 
            340                 345                 350         


Thr Tyr Gly Pro Leu Gln Gly Gly Cys Lys Gln Ser Val Phe Ser Gly 
        355                 360                 365             


Lys Ala Thr Cys Cys Tyr Ala Tyr Ser Tyr Lys Gly Pro Met Ala Cys 
    370                 375                 380                 


Lys Gly Val Tyr Ser Gly Glu Leu Ser Thr Asn Phe Glu Cys Gly Leu 
385                 390                 395                 400 


Leu Val Tyr Val Thr Lys Ser Asp Gly Ser Arg Ile Gln Thr Arg Thr 
                405                 410                 415     


Glu Pro Leu Val Leu Thr Gln Tyr Asn Tyr Asn Asn Ile Thr Leu Asp 
            420                 425                 430         


Lys Cys Val Ala Tyr Asn Ile Tyr Gly Arg Val Gly Gln Gly Phe Ile 
        435                 440                 445             


Thr Asn Val Thr Asp Ser Ala Ala Asn Phe Ser Tyr Leu Ala Asp Gly 
    450                 455                 460                 


Gly Leu Ala Ile Leu Asp Thr Ser Gly Ala Ile Asp Val Phe Val Val 
465                 470                 475                 480 


Gln Gly Ile Tyr Gly Leu Asn Tyr Tyr Lys Val Asn Pro Cys Glu Asp 
                485                 490                 495     


Val Asn Gln Gln Phe Val Val Ser Gly Gly Asn Ile Val Gly Ile Leu 
            500                 505                 510         


Thr Ser Arg Asn Glu Thr Gly Ser Glu Gln Val Glu Asn Gln Phe Tyr 
        515                 520                 525             


Val Lys Leu Thr Asn Ser Ser His Arg Arg Arg Arg Ser Ile Gly Gln 
    530                 535                 540                 


Asn Val Thr Ser Cys Pro Tyr Val Ser Tyr Gly Arg Phe Cys Ile Glu 
545                 550                 555                 560 


Pro Asp Gly Ser Leu Lys Met 
                565         


<210>  4
<211>  582
<212>  PRT
<213>  Infectious Bronchitis Virus QX Like 1449-10

<400>  4

Met Leu Val Lys Ser Leu Phe Leu Val Thr Ile Leu Cys Ala Leu Cys 
1               5                   10                  15      


Ser Ala Asn Leu Phe Asp Ser Asp Asn Asn Tyr Val Tyr Tyr Tyr Gln 
            20                  25                  30          


Ser Ala Phe Arg Pro Pro Asn Gly Trp His Leu Gln Gly Gly Ala Tyr 
        35                  40                  45              


Ala Val Val Asn Ser Thr Asn Tyr Thr Asn Asn Ala Gly Ser Ala His 
    50                  55                  60                  


Glu Cys Thr Val Gly Val Ile Lys Asp Val Tyr Asn Gln Ser Val Ala 
65                  70                  75                  80  


Ser Ile Ala Met Thr Ala Pro Leu Gln Gly Met Ala Trp Ser Lys Ser 
                85                  90                  95      


Gln Phe Cys Ser Ala His Cys Asn Phe Ser Glu Ile Thr Val Phe Val 
            100                 105                 110         


Thr His Cys Tyr Ser Gly Gly Ser Gly Ser Cys Pro Ile Thr Gly Met 
        115                 120                 125             


Ile Pro Arg Asp His Ile Arg Ile Ser Ala Met Lys Asn Gly Ser Leu 
    130                 135                 140                 


Phe Tyr Asn Leu Thr Val Ser Val Ser Lys Tyr Pro Asn Phe Lys Ser 
145                 150                 155                 160 


Phe Gln Cys Val Asn Asn Phe Thr Ser Val Tyr Leu Asn Gly Asp Leu 
                165                 170                 175     


Val Phe Thr Ser Asn Lys Thr Thr Asp Val Thr Ser Ala Gly Val Tyr 
            180                 185                 190         


Phe Lys Ala Gly Gly Pro Val Asn Tyr Ser Ile Met Lys Glu Phe Lys 
        195                 200                 205             


Val Leu Ala Tyr Phe Val Asn Gly Thr Ala Gln Asp Val Ile Leu Cys 
    210                 215                 220                 


Asp Asn Ser Pro Lys Gly Leu Leu Ala Cys Gln Tyr Asn Thr Gly Asn 
225                 230                 235                 240 


Phe Ser Asp Gly Phe Tyr Pro Phe Thr Asn Ser Thr Leu Val Arg Glu 
                245                 250                 255     


Lys Phe Ile Val Tyr Arg Glu Ser Ser Val Asn Thr Thr Leu Ala Leu 
            260                 265                 270         


Thr Asn Phe Thr Phe Thr Asn Val Ser Asn Ala Gln Pro Asn Ser Gly 
        275                 280                 285             


Gly Val Asn Thr Phe His Leu Tyr Gln Thr Gln Thr Ala Gln Ser Gly 
    290                 295                 300                 


Tyr Tyr Asn Phe Asn Leu Ser Phe Leu Ser Gln Phe Val Tyr Lys Ala 
305                 310                 315                 320 


Ser Asp Phe Met Tyr Gly Ser Tyr His Pro Ser Cys Ser Phe Arg Pro 
                325                 330                 335     


Glu Thr Ile Asn Ser Gly Leu Trp Phe Asn Ser Leu Ser Val Ser Leu 
            340                 345                 350         


Thr Tyr Gly Pro Leu Gln Gly Gly Cys Lys Gln Ser Val Phe Ser Gly 
        355                 360                 365             


Lys Ala Thr Cys Cys Tyr Ala Tyr Ser Tyr Lys Gly Pro Met Ala Cys 
    370                 375                 380                 


Lys Gly Val Tyr Ser Gly Glu Leu Ser Thr Asn Phe Glu Cys Gly Leu 
385                 390                 395                 400 


Leu Val Tyr Val Thr Lys Ser Asp Gly Ser Arg Ile Gln Thr Arg Thr 
                405                 410                 415     


Glu Pro Leu Val Leu Thr Gln Tyr Asn Tyr Asn Asn Ile Thr Leu Asp 
            420                 425                 430         


Lys Cys Val Ala Tyr Asn Ile Tyr Gly Arg Val Gly Gln Gly Phe Ile 
        435                 440                 445             


Thr Asn Val Thr Asp Ser Ala Ala Asn Phe Ser Tyr Leu Ala Asp Gly 
    450                 455                 460                 


Gly Leu Ala Ile Leu Asp Thr Ser Gly Ala Ile Asp Val Phe Val Val 
465                 470                 475                 480 


Gln Gly Ile Tyr Gly Leu Asn Tyr Tyr Lys Val Asn Pro Cys Glu Asp 
                485                 490                 495     


Val Asn Gln Gln Phe Val Val Ser Gly Gly Asn Ile Val Gly Ile Leu 
            500                 505                 510         


Thr Ser Arg Asn Glu Thr Gly Ser Glu Gln Val Glu Asn Gln Phe Tyr 
        515                 520                 525             


Val Lys Leu Thr Asn Ser Ser His Arg Arg Arg Arg Ser Ile Gly Gln 
    530                 535                 540                 


Asn Val Thr Ser Cys Pro Tyr Val Ser Tyr Gly Arg Phe Cys Ile Glu 
545                 550                 555                 560 


Pro Asp Gly Ser Leu Lys Met Ile Val Pro Glu Glu Leu Lys Gln Phe 
                565                 570                 575     


Val Ala Pro Leu Leu Asn 
            580         


<210>  5
<211>  24
<212>  DNA
<213>  Infectious Bronchitis Virus primer

<400>  5
taatactggy aatttttcag atgg                                            24


<210>  6
<211>  22
<212>  DNA
<213>  Infectious Bronchitis Virus primer

<400>  6
aatacagatt gcttacacca cc                                              22


<210>  7
<211>  18
<212>  DNA
<213>  Infectious Bronchitis Virus primer

<400>  7
gcttatgcag tagtcaat                                                   18


<210>  8
<211>  22
<212>  DNA
<213>  Infectious Bronchitis Virus primer

<400>  8
cacgtggaat catgcctgtt at                                              22


