                               SEQUENCE LISTING

<110> THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

<120> MULLERIAN INHIBITING SUBSTANCE (MIS) ANALOGUES

<130> 19240.852.WO1

<140> PCT/US09/52842
<141> 2009-08-05

<150> 61/137,948
<151> 2008-08-05

<160> 7     

<170> PatentIn version 3.5

<210> 1
<211> 605
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polypeptide

<400> 1
Met Gln Gly Pro His Leu Ser Leu Leu Leu Leu Leu Leu Ala Thr Met 
1               5                   10                  15      


Gly Ala Val Leu Gln Ala Asp Thr Val Glu Glu Leu Thr Asn Thr Arg 
            20                  25                  30          


Gly Leu Ile Phe Leu Glu Asp Gly Val Trp Pro Pro Ser Ser Pro Pro 
        35                  40                  45              


Glu Pro Leu Cys Leu Val Ala Val Arg Gly Glu Gly Asp Thr Ser Lys 
    50                  55                  60                  


Ala Ser Leu Thr Val Val Gly Gly Leu His Ser Tyr Glu His Ala Phe 
65                  70                  75                  80  


Leu Glu Ala Val Gln Glu Ser Arg Trp Gly Pro Gln Asp Leu Ala Thr 
                85                  90                  95      


Phe Gly Val Cys Ser Thr Asp Ser Gln Thr Thr Leu Pro Ala Leu Gln 
            100                 105                 110         


Arg Leu Gly Ala Trp Leu Gly Glu Thr Gly Glu Gln Gln Leu Leu Val 
        115                 120                 125             


Leu His Leu Ala Glu Val Ile Trp Glu Pro Gln Leu Leu Leu Lys Phe 
    130                 135                 140                 


Gln Glu Pro Pro Pro Gly Gly Ala Ser Arg Trp Glu Gln Ala Leu Leu 
145                 150                 155                 160 


Val Leu Tyr Pro Gly Pro Gly Pro Gln Val Thr Val Thr Gly Ala Gly 
                165                 170                 175     


Leu Gln Gly Thr Gln Ser Leu Cys Pro Thr Arg Asp Thr Arg Tyr Leu 
            180                 185                 190         


Val Leu Thr Val His Phe Pro Ala Gly Ala Trp Ser Gly Ser Gly Leu 
        195                 200                 205             


Ala Leu Thr Leu Gln Pro Ser Lys Glu Gly Ala Thr Leu Thr Ile Ala 
    210                 215                 220                 


Gln Leu Gln Ala Phe Leu Phe Gly Ser Asp Ser Arg Cys Phe Thr Arg 
225                 230                 235                 240 


Met Thr Pro Thr Leu Val Leu Leu Pro Pro Thr Gly Pro Thr Pro Gln 
                245                 250                 255     


Pro Ala His Gly Gln Leu Asp Thr Val Pro Phe Pro Gln Pro Gly Leu 
            260                 265                 270         


Ser Leu Glu Pro Glu Asp Leu Pro His Ser Ala Asp Pro Phe Leu Glu 
        275                 280                 285             


Thr Leu Thr Arg Leu Val Arg Ala Leu Arg Gly Pro Leu Thr Arg Ala 
    290                 295                 300                 


Ser Asn Thr Arg Leu Ala Leu Asp Pro Gly Ala Leu Ala Ser Phe Pro 
305                 310                 315                 320 


Gln Gly Leu Val Asn Leu Ser Asp Pro Val Ala Leu Gly Arg Leu Leu 
                325                 330                 335     


Asp Gly Glu Glu Pro Leu Leu Leu Leu Leu Ser Pro Ala Ala Ala Thr 
            340                 345                 350         


Val Gly Glu Pro Met Arg Leu His Ser Pro Thr Ser Ala Pro Trp Ala 
        355                 360                 365             


Ala Gly Leu Ala Arg Arg Val Ala Val Glu Leu Gln Ala Ala Ala Ser 
    370                 375                 380                 


Glu Leu Arg Asp Leu Pro Gly Leu Pro Pro Thr Ala Pro Pro Leu Leu 
385                 390                 395                 400 


Ser Arg Leu Leu Ala Leu Cys Pro Asn Asp Ser Arg Ser Ala Gly Asp 
                405                 410                 415     


Pro Leu Arg Ala Leu Leu Leu Leu Lys Ala Leu Gln Gly Leu Arg Ala 
            420                 425                 430         


Glu Trp Arg Gly Arg Glu Gly Arg Gly Ser Asn Ala Thr Gly Ser Gly 
        435                 440                 445             


Ser Asn Ala Thr Ser Arg Ser Gly Ser Asn Ala Thr Gly Ser Gly Ser 
    450                 455                 460                 


Asn Ala Thr Ser Phe Glu Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly 
465                 470                 475                 480 


Leu Asp Ser Thr Arg Thr Gly His His His His His His Arg Ala Arg 
                485                 490                 495     


Arg Ser Lys Gly Thr Gly Thr Asp Gly Leu Cys Ala Leu Arg Glu Leu 
            500                 505                 510         


Ser Val Asp Leu Arg Ala Glu Arg Ser Val Leu Ile Pro Glu Thr Tyr 
        515                 520                 525             


Gln Ala Asn Asn Cys Gln Gly Ala Cys Ala Trp Pro Gln Ser Asp Arg 
    530                 535                 540                 


Asn Pro Arg Tyr Gly Asn His Val Val Leu Leu Leu Lys Met Gln Ala 
545                 550                 555                 560 


Arg Gly Ala Ala Leu Gly Arg Leu Pro Cys Cys Val Pro Thr Ala Tyr 
                565                 570                 575     


Thr Gly Lys Leu Leu Ile Ser Leu Ser Glu Glu His Ile Ser Ala His 
            580                 585                 590         


His Val Pro Asn Met Val Ala Thr Glu Cys Gly Cys Arg 
        595                 600                 605 


<210> 2
<211> 2065
<212> DNA
<213> Homo sapiens

<400> 2
gcatgttgac acatcaggcc cagctctatc actggggagg gagataggct gccagggaca       60

gaaagggctc tttgagaagg ccactctgcc tggagtgggg gcgccgggca ctgtccccca      120

aggtcgcggc agaggagata ggggtctgtc ctgcacaaac accccacctt ccactcggct      180

cacttaaggc aggcagccca gcccctggca gcacccacga tgcgggacct gcctctcacc      240

agcctggccc tagtgctgtc tgccctgggg gctctgctgg ggactgaggc cctcagagca      300

gaggagccag ctgtgggcac cagtggcctc atcttccgag aagacttgga ctggcctcca      360

ggcagcccac aagagcctct gtgcctggtg gcactgggcg gggacagcaa tggcagcagc      420

tcccccctgc gggtggtggg ggctctaagc gcctatgagc aggccttcct gggggccgtg      480

cagagggccc gctggggccc ccgagacctg gccaccttcg gggtctgcaa caccggtgac      540

aggcaggctg ccttgccctc tctacggcgg ctgggggcct ggctgcggga ccctgggggg      600

cagcgcctgg tggtcctaca cctggaggaa gtgacctggg agccaacacc ctcgctgagg      660

ttccaggagc ccccgcctgg aggagctggc cccccagagc tggcgctgct ggtgctgtac      720

cctgggcctg gccctgaggt cactgtgacg agggctgggc tgccgggtgc ccagagcctc      780

tgcccctccc gagacacccg ctacctggtg ttagcggtgg accgccctgc gggggcctgg      840

cgcggctccg ggctggcctt gaccctgcag ccccgcggag aggactcccg gctgagtacc      900

gcccggctgc aggcactgct gttcggcgac gaccaccgct gcttcacacg gatgaccccg      960

gccctgctcc tgctgccgcg gtccgagccc gcgccgctgc ctgcgcacgg ccagctggac     1020

accgtgccct tcccgccgcc caggccatcc gcggaactcg aggagtcgcc acccagcgca     1080

gaccccttcc tggagacgct cacgcgcctg gtgcgggcgc tgcgggtccc cccggcccgg     1140

gcctccgcgc cgcgcctggc cctggatccg gacgcgctgg ccggcttccc gcagggccta     1200

gtcaacctgt cggaccccgc ggcgctggag cgcctactcg acggcgagga gccgctgctg     1260

ctgctgctga ggcccactgc ggccaccacc ggggatcctg cgcccctgca cgaccccacg     1320

tcggcgccgt gggccacggc cctggcgcgc cgcgtggctg ctgaactgca agcggcggct     1380

gccgagctgc gaagcctccc gggtctgcct ccggccacag ccccgctgct ggcgcgcctg     1440

ctcgcgctct gcccaggtgg ccccggcggc ctcggcgatc ccctgcgagc gctgctgctc     1500

ctgaaggcgc tgcagggcct gcgcgtggag tggcgcgggc gggatccgcg cgggccgggt     1560

cgggcacagc gcagcgcggg ggccaccgcc gccgacgggc cgtgcgcgct gcgcgagctc     1620

agcgtagacc tccgcgccga gcgctccgta ctcatccccg agacctacca ggccaacaat     1680

tgccagggcg tgtgcggctg gcctcagtcc gaccgcaacc cgcgctacgg caaccacgtg     1740

gtgctgctgc tgaagatgca ggcccgtggg gccgccctgg cgcgcccacc ctgctgcgtg     1800

cccaccgcct acgcgggcaa gctgctcatc agcctgtcgg aggagcgcat cagcgcgcac     1860

cacgtgccca acatggtggc caccgagtgt ggctgccggt gacccctgcg ccgcgcggac     1920

tcctgccccg agggtccgga cgcgccccag ctcgcgcccc ttcccatatt tattcggacc     1980

ccaagcatcg ccccaataaa gaccagcaag caaccggcaa aaaaaaaaaa aaaaaaaaaa     2040

aaaaaaaaaa aaaaaaaaaa aaaaa                                           2065


<210> 3
<211> 560
<212> PRT
<213> Homo sapiens

<400> 3
Met Arg Asp Leu Pro Leu Thr Ser Leu Ala Leu Val Leu Ser Ala Leu 
1               5                   10                  15      


Gly Ala Leu Leu Gly Thr Glu Ala Leu Arg Ala Glu Glu Pro Ala Val 
            20                  25                  30          


Gly Thr Ser Gly Leu Ile Phe Arg Glu Asp Leu Asp Trp Pro Pro Gly 
        35                  40                  45              


Ile Pro Gln Glu Pro Leu Cys Leu Val Ala Leu Gly Gly Asp Ser Asn 
    50                  55                  60                  


Gly Ser Ser Ser Pro Leu Arg Val Val Gly Ala Leu Ser Ala Tyr Glu 
65                  70                  75                  80  


Gln Ala Phe Leu Gly Ala Val Gln Arg Ala Arg Trp Gly Pro Arg Asp 
                85                  90                  95      


Leu Ala Thr Phe Gly Val Cys Asn Thr Gly Asp Arg Gln Ala Ala Leu 
            100                 105                 110         


Pro Ser Leu Arg Arg Leu Gly Ala Trp Leu Arg Asp Pro Gly Gly Gln 
        115                 120                 125             


Arg Leu Val Val Leu His Leu Glu Glu Val Thr Trp Glu Pro Thr Pro 
    130                 135                 140                 


Ser Leu Arg Phe Gln Glu Pro Pro Pro Gly Gly Ala Gly Pro Pro Glu 
145                 150                 155                 160 


Leu Ala Leu Leu Val Leu Tyr Pro Gly Pro Gly Pro Glu Val Thr Val 
                165                 170                 175     


Thr Arg Ala Gly Leu Pro Gly Ala Gln Ser Leu Cys Pro Ser Arg Asp 
            180                 185                 190         


Thr Arg Tyr Leu Val Leu Ala Val Asp Arg Pro Ala Gly Ala Trp Arg 
        195                 200                 205             


Gly Ser Gly Leu Ala Leu Thr Leu Gln Pro Arg Gly Glu Asp Ser Arg 
    210                 215                 220                 


Leu Ser Thr Ala Arg Leu Gln Ala Leu Leu Phe Gly Asp Asp His Arg 
225                 230                 235                 240 


Cys Phe Thr Arg Met Thr Pro Ala Leu Leu Leu Leu Pro Arg Ser Glu 
                245                 250                 255     


Pro Ala Pro Leu Pro Ala His Gly Gln Leu Asp Thr Val Pro Phe Pro 
            260                 265                 270         


Pro Pro Arg Pro Ser Ala Glu Leu Glu Glu Ser Pro Pro Ser Ala Asp 
        275                 280                 285             


Pro Phe Leu Glu Thr Leu Thr Arg Leu Val Arg Ala Leu Arg Val Pro 
    290                 295                 300                 


Pro Ala Arg Ala Ser Ala Pro Arg Leu Ala Leu Asp Pro Asp Ala Leu 
305                 310                 315                 320 


Ala Gly Phe Pro Gln Gly Leu Val Asn Leu Ser Asp Pro Ala Ala Leu 
                325                 330                 335     


Glu Arg Leu Leu Asp Gly Glu Glu Pro Leu Leu Leu Leu Leu Arg Pro 
            340                 345                 350         


Thr Ala Ala Thr Thr Gly Asp Pro Ala Pro Leu His Asp Pro Thr Ser 
        355                 360                 365             


Ala Pro Trp Ala Thr Ala Leu Ala Arg Arg Val Ala Ala Glu Leu Gln 
    370                 375                 380                 


Ala Ala Ala Ala Glu Leu Arg Ser Leu Pro Gly Leu Pro Pro Ala Thr 
385                 390                 395                 400 


Ala Pro Leu Leu Ala Arg Leu Leu Ala Leu Cys Pro Gly Gly Pro Gly 
                405                 410                 415     


Gly Leu Gly Asp Pro Leu Arg Ala Leu Leu Leu Leu Lys Ala Leu Gln 
            420                 425                 430         


Gly Leu Arg Val Glu Trp Arg Gly Arg Asp Pro Arg Gly Pro Gly Arg 
        435                 440                 445             


Ala Gln Arg Ser Ala Gly Ala Thr Ala Ala Asp Gly Pro Cys Ala Leu 
    450                 455                 460                 


Arg Glu Leu Ser Val Asp Leu Arg Ala Glu Arg Ser Val Leu Ile Pro 
465                 470                 475                 480 


Glu Thr Tyr Gln Ala Asn Asn Cys Gln Gly Val Cys Gly Trp Pro Gln 
                485                 490                 495     


Ser Asp Arg Asn Pro Arg Tyr Gly Asn His Val Val Leu Leu Leu Lys 
            500                 505                 510         


Met Gln Val Arg Gly Ala Ala Leu Ala Arg Pro Pro Cys Cys Val Pro 
        515                 520                 525             


Thr Ala Tyr Ala Gly Lys Leu Leu Ile Ser Leu Ser Glu Glu Arg Ile 
    530                 535                 540                 


Ser Ala His His Val Pro Asn Met Val Ala Thr Glu Cys Gly Cys Arg 
545                 550                 555                 560 


<210> 4
<211> 14
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic peptide

<400> 4
Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210> 5
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic 6xHis tag

<400> 5
His His His His His His 
1               5       


<210> 6
<211> 108
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic polypeptide

<400> 6
Ser Lys Gly Thr Gly Thr Asp Gly Leu Cys Ala Leu Arg Glu Leu Ser 
1               5                   10                  15      


Val Asp Leu Arg Ala Glu Arg Ser Val Leu Ile Pro Glu Thr Tyr Gln 
            20                  25                  30          


Ala Asn Asn Cys Gln Gly Ala Cys Ala Trp Pro Gln Ser Asp Arg Asn 
        35                  40                  45              


Pro Arg Tyr Gly Asn His Val Val Leu Leu Leu Lys Met Gln Ala Arg 
    50                  55                  60                  


Gly Ala Ala Leu Gly Arg Leu Pro Cys Cys Val Pro Thr Ala Tyr Thr 
65                  70                  75                  80  


Gly Lys Leu Leu Ile Ser Leu Ser Glu Glu His Ile Ser Ala His His 
                85                  90                  95      


Val Pro Asn Met Val Ala Thr Glu Cys Gly Cys Arg 
            100                 105             


<210> 7
<211> 109
<212> PRT
<213> Homo sapiens

<400> 7
Ser Ala Gly Ala Thr Ala Ala Asp Gly Pro Cys Ala Leu Arg Glu Leu 
1               5                   10                  15      


Ser Val Asp Leu Arg Ala Glu Arg Ser Val Leu Ile Pro Glu Thr Tyr 
            20                  25                  30          


Gln Ala Asn Asn Cys Gln Gly Val Cys Gly Trp Pro Gln Ser Asp Arg 
        35                  40                  45              


Asn Pro Arg Tyr Gly Asn His Val Val Leu Leu Leu Lys Met Gln Val 
    50                  55                  60                  


Arg Gly Ala Ala Leu Ala Arg Pro Pro Cys Cys Val Pro Thr Ala Tyr 
65                  70                  75                  80  


Ala Gly Lys Leu Leu Ile Ser Leu Ser Glu Glu Arg Ile Ser Ala His 
                85                  90                  95      


His Val Pro Asn Met Val Ala Thr Glu Cys Gly Cys Arg 
            100                 105                 


