                         SEQUENCE LISTING

<110>  TOSOH CORPORATION

<120>  RNA Polymerase Mutant With Improved Function

<130>  W708-PCT

<150>  JP2008-205491
<151>  2008-08-08

<150>  JP2009-038979
<151>  2009-02-23

<150>  JP2009-038980
<151>  2009-02-23

<160>  59


<210>  1
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  1
gcacttcatg aacacgatta acatcgc                                           27


<210>  2
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  2
agtgccaagc ttgactttct cagagat                                           27


<210>  3
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  3
gtcaagcttg gcactaaggc actggctgg                                         29


<210>  4
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  4
ctagataagc tttacgcgaa cgcgaagtcc g                                      31


<210>  5
<211>  2652
<212>  DNA
<213>  Bacteriophage T7

<400>  5
atg aac acg att aac atc gct aag aac gac ttc tct gac atc gaa ctg         48
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
gct gct atc ccg ttc aac act ctg gct gac cat tac ggt gag cgt tta         96
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
gct cgc gaa cag ttg gcc ctt gag cat gag tct tac gag atg ggt gaa        144
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
gca cgc ttc cgc aag atg ttt gag cgt caa ctt aaa gct ggt gag gtt        192
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
gcg gat aac gct gcc gcc aag cct ctc atc act acc cta ctc cct aag        240
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
atg att gca cgc atc aac gac tgg ttt gag gaa gtg aaa gct aag cgc        288
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
ggc aag cgc ccg aca gcc ttc cag ttc ctg caa gaa atc aag ccg gaa        336
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
gcc gta gcg tac atc acc att aag acc act ctg gct tgc cta acc agt        384
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
gct gac aat aca acc gtt cag gct gta gca agc gca atc ggt cgg gcc        432
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
att gag gac gag gct cgc ttc ggt cgt atc cgt gac ctt gaa gct aag        480
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
cac ttc aag aaa aac gtt gag gaa caa ctc aac aag cgc gta ggg cac        528
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
gtc tac aag aaa gca ttt atg caa gtt gtc gag gct gac atg ctc tct        576
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
aag ggt cta ctc ggt ggc gag gcg tgg tct tcg tgg cat aag gaa gac        624
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
tct att cat gta gga gta cgc tgc atc gag atg ctc att gag tca acc        672
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
gga atg gtt agc tta cac cgc caa aat gct ggc gta gta ggt caa gac        720
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
tct gag act atc gaa ctc gca cct gaa tac gct gag gct atc gca acc        768
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
cgt gca ggt gcg ctg gct ggc atc tct ccg atg ttc caa cct tgc gta        816
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
gtt cct cct aag ccg tgg act ggc att act ggt ggt ggc tat tgg gct        864
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
aac ggt cgt cgt cct ctg gcg ctg gtg cgt act cac agt aag aaa gca        912
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
ctg atg cgc tac gaa gac gtt tac atg cct gag gtg tac aaa gcg att        960
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
aac att gcg caa aac acc gca tgg aaa atc aac aag aaa gtc cta gcg       1008
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
gtc gcc aac gta atc acc aag tgg aag cat tgt ccg gtc gag gac atc       1056
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
cct gcg att gag cgt gaa gaa ctc ccg atg aaa ccg gaa gac atc gac       1104
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
atg aat cct gag gct ctc acc gcg tgg aaa cgt gct gcc gct gct gtg       1152
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
tac cgc aag gac aag gct cgc aag tct cgc cgt atc agc ctt gag ttc       1200
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
atg ctt gag caa gcc aat aag ttt gct aac cat aag gcc atc tgg ttc       1248
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
cct tac aac atg gac tgg cgc ggt cgt gtt tac gct gtg tca atg ttc       1296
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
aac ccg caa ggt aac gat atg acc aaa gga ctg ctt acg ctg gcg aaa       1344
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
ggt aaa cca atc ggt aag gaa ggt tac tac tgg ctg aaa atc cac ggt       1392
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
gca aac tgt gcg ggt gtc gat aag gtt ccg ttc cct gag cgc atc aag       1440
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
ttc att gag gaa aac cac gag aac atc atg gct tgc gct aag tct cca       1488
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
ctg gag aac act tgg tgg gct gag caa gat tct ccg ttc tgc ttc ctt       1536
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
gcg ttc tgc ttt gag tac gct ggg gta cag cac cac ggc ctg agc tat       1584
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
aac tgc tcc ctt ccg ctg gcg ttt gac ggg tct tgc tct ggc atc cag       1632
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
cac ttc tcc gcg atg ctc cga gat gag gta ggt ggt cgc gcg gtt aac       1680
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
ttg ctt cct agt gaa acc gtt cag gac atc tac ggg att gtt gct aag       1728
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
aaa gtc aac gag att cta caa gca gac gca atc aat ggg acc gat aac       1776
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
gaa gta gtt acc gtg acc gat gag aac act ggt gaa atc tct gag aaa       1824
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
gtc aag ctt ggc act aag gca ctg gct ggt caa tgg ctg gct tac ggt       1872
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
gtt act cgc agt gtg act aag cgt tca gtc atg acg ctg gct tac ggg       1920
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
tcc aaa gag ttc ggc ttc cgt caa caa gtg ctg gaa gat acc att cag       1968
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
cca gct att gat tcc ggc aag ggt ctg atg ttc act cag ccg aat cag       2016
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
gct gct gga tac atg gct aag ctg att tgg gaa tct gtg agc gtg acg       2064
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr
        675                 680                 685
gtg gta gct gcg gtt gaa gca atg aac tgg ctt aag tct gct gct aag       2112
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
ctt ctg gct gct gag gtc aaa gat aag aag act gga gag att ctt cgc       2160
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
aag cgt tgc gct gtg cat tgg gta act cct gat ggt ttc cct gtg tgg       2208
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
cag gaa tac aag aag cct att cag acg cgc ttg aac ctg atg ttc ctc       2256
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
ggt cag ttc cgc tta cag cct acc att aac acc aac aaa gat agc gag       2304
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
att gat gca cac aaa cag gag tct ggt atc gct cct aac ttt gta cac       2352
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
agc caa gac ggt agc cac ctt cgt aag act gta gtg tgg gca cac gag       2400
Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
aag tac gga atc gaa tct ttt gca ctg att cac gac tcc ttc ggt acc       2448
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
att ccg gct gac gct gcg aac ctg ttc aaa gca gtg cgc gaa act atg       2496
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
gtt gac aca tat gag tct tgt gat gta ctg gct gat ttc tac gac cag       2544
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
ttc gct gac cag ttg cac gag tct caa ttg gac aaa atg cca gca ctt       2592
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
ccg gct aaa ggt aac ttg aac ctc cgt gac atc tta gag tcg gac ttc       2640
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
gcg ttc gcg taa                                                       2652
Ala Phe Ala


<210>  6
<211>  883
<212>  PRT
<213>  Bacteriophage T7

<400>  6
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr
        675                 680                 685
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
Ala Phe Ala


<210>  7
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  7
cacgatgcat ctgaaatgag ctgttgac                                          28


<210>  8
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  8
atacctaggc tgcagctacg cgaacgcgaa                                        30


<210>  9
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  9
atggggcatc atcatcatca tcatatgaac acgattaaca tc                          42


<210>  10
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  10
gtcgatgtct tccggtttca tcggg                                             25


<210>  11
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  11
atgatgatga tgatgatgcc ccatggtctg tttcctgtgt g                           41


<210>  12
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  12
gcttccacag caatggcatc ctggtc                                            26


<210>  13
<211>  2676
<212>  DNA
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase gene

<400>  13
atg ggg cat cat cat cat cat cat atg aac acg att aac atc gct aag         48
Met Gly His His His His His His Met Asn Thr Ile Asn Ile Ala Lys
1               5                   10                  15
aac gac ttc tct gac atc gaa ctg gct gct atc ccg ttc aac act ctg         96
Asn Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu
            20                  25                  30
gct gac cat tac ggt gag cgt tta gct cgc gaa cag ttg gcc ctt gag        144
Ala Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu
        35                  40                  45
cat gag tct tac gag atg ggt gaa gca cgc ttc cgc aag atg ttt gag        192
His Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu
    50                  55                  60
cgt caa ctt aaa gct ggt gag gtt gcg gat aac gct gcc gcc aag cct        240
Arg Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro
65                  70                  75                  80
ctc atc act acc cta ctc cct aag atg att gca cgc atc aac gac tgg        288
Leu Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp
                85                  90                  95
ttt gag gaa gtg aaa gct aag cgc ggc aag cgc ccg aca gcc ttc cag        336
Phe Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln
            100                 105                 110
ttc ctg caa gaa atc aag ccg gaa gcc gta gcg tac atc acc att aag        384
Phe Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys
        115                 120                 125
acc act ctg gct tgc cta acc agt gct gac aat aca acc gtt cag gct        432
Thr Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala
    130                 135                 140
gta gca agc gca atc ggt cgg gcc att gag gac gag gct cgc ttc ggt        480
Val Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly
145                 150                 155                 160
cgt atc cgt gac ctt gaa gct aag cac ttc aag aaa aac gtt gag gaa        528
Arg Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu
                165                 170                 175
caa ctc aac aag cgc gta ggg cac gtc tac aag aaa gca ttt atg caa        576
Gln Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln
            180                 185                 190
gtt gtc gag gct gac atg ctc tct aag ggt cta ctc ggt ggc gag gcg        624
Val Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala
        195                 200                 205
tgg tct tcg tgg cat aag gaa gac tct att cat gta gga gta cgc tgc        672
Trp Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys
    210                 215                 220
atc gag atg ctc att gag tca acc gga atg gtt agc tta cac cgc caa        720
Ile Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln
225                 230                 235                 240
aat gct ggc gta gta ggt caa gac tct gag act atc gaa ctc gca cct        768
Asn Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro
                245                 250                 255
gaa tac gct gag gct atc gca acc cgt gca ggt gcg ctg gct ggc atc        816
Glu Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile
            260                 265                 270
tct ccg atg ttc caa cct tgc gta gtt cct cct aag ccg tgg act ggc        864
Ser Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly
        275                 280                 285
att act ggt ggt ggc tat tgg gct aac ggt cgt cgt cct ctg gcg ctg        912
Ile Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu
    290                 295                 300
gtg cgt act cac agt aag aaa gca ctg atg cgc tac gaa gac gtt tac        960
Val Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr
305                 310                 315                 320
atg cct gag gtg tac aaa gcg att aac att gcg caa aac acc gca tgg       1008
Met Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp
                325                 330                 335
aaa atc aac aag aaa gtc cta gcg gtc gcc aac gta atc acc aag tgg       1056
Lys Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp
            340                 345                 350
aag cat tgt ccg gtc gag gac atc cct gcg att gag cgt gaa gaa ctc       1104
Lys His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu
        355                 360                 365
ccg atg aaa ccg gaa gac atc gac atg aat cct gag gct ctc acc gcg       1152
Pro Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala
    370                 375                 380
tgg aaa cgt gct gcc gct gct gtg tac cgc aag gac aag gct cgc aag       1200
Trp Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys
385                 390                 395                 400
tct cgc cgt atc agc ctt gag ttc atg ctt gag caa gcc aat aag ttt       1248
Ser Arg Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe
                405                 410                 415
gct aac cat aag gcc atc tgg ttc cct tac aac atg gac tgg cgc ggt       1296
Ala Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly
            420                 425                 430
cgt gtt tac gct gtg tca atg ttc aac ccg caa ggt aac gat atg acc       1344
Arg Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr
        435                 440                 445
aaa gga ctg ctt acg ctg gcg aaa ggt aaa cca atc ggt aag gaa ggt       1392
Lys Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly
    450                 455                 460
tac tac tgg ctg aaa atc cac ggt gca aac tgt gcg ggt gtc gat aag       1440
Tyr Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys
465                 470                 475                 480
gtt ccg ttc cct gag cgc atc aag ttc att gag gaa aac cac gag aac       1488
Val Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn
                485                 490                 495
atc atg gct tgc gct aag tct cca ctg gag aac act tgg tgg gct gag       1536
Ile Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu
            500                 505                 510
caa gat tct ccg ttc tgc ttc ctt gcg ttc tgc ttt gag tac gct ggg       1584
Gln Asp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly
        515                 520                 525
gta cag cac cac ggc ctg agc tat aac tgc tcc ctt ccg ctg gcg ttt       1632
Val Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe
    530                 535                 540
gac ggg tct tgc tct ggc atc cag cac ttc tcc gcg atg ctc cga gat       1680
Asp Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp
545                 550                 555                 560
gag gta ggt ggt cgc gcg gtt aac ttg ctt cct agt gaa acc gtt cag       1728
Glu Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln
                565                 570                 575
gac atc tac ggg att gtt gct aag aaa gtc aac gag att cta caa gca       1776
Asp Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala
            580                 585                 590
gac gca atc aat ggg acc gat aac gaa gta gtt acc gtg acc gat gag       1824
Asp Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu
        595                 600                 605
aac act ggt gaa atc tct gag aaa gtc aag ctt ggc act aag gca ctg       1872
Asn Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu
    610                 615                 620
gct ggt caa tgg ctg gct tac ggt gtt act cgc agt gtg act aag cgt       1920
Ala Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg
625                 630                 635                 640
tca gtc atg acg ctg gct tac ggg tcc aaa gag ttc ggc ttc cgt caa       1968
Ser Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln
                645                 650                 655
caa gtg ctg gaa gat acc att cag cca gct att gat tcc ggc aag ggt       2016
Gln Val Leu Glu Asp Thr Ile Gln Pro Ala Ile Asp Ser Gly Lys Gly
            660                 665                 670
ctg atg ttc act cag ccg aat cag gct gct gga tac atg gct aag ctg       2064
Leu Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu
        675                 680                 685
att tgg gaa tct gtg agc gtg acg gtg gta gct gcg gtt gaa gca atg       2112
Ile Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met
    690                 695                 700
aac tgg ctt aag tct gct gct aag ctt ctg gct gct gag gtc aaa gat       2160
Asn Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp
705                 710                 715                 720
aag aag act gga gag att ctt cgc aag cgt tgc gct gtg cat tgg gta       2208
Lys Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val
                725                 730                 735
act cct gat ggt ttc cct gtg tgg cag gaa tac aag aag cct att cag       2256
Thr Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln
            740                 745                 750
acg cgc ttg aac ctg atg ttc ctc ggt cag ttc cgc tta cag cct acc       2304
Thr Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr
        755                 760                 765
att aac acc aac aaa gat agc gag att gat gca cac aaa cag gag tct       2352
Ile Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser
    770                 775                 780
ggt atc gct cct aac ttt gta cac agc caa gac ggt agc cac ctt cgt       2400
Gly Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg
785                 790                 795                 800
aag act gta gtg tgg gca cac gag aag tac gga atc gaa tct ttt gca       2448
Lys Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala
                805                 810                 815
ctg att cac gac tcc ttc ggt acc att ccg gct gac gct gcg aac ctg       2496
Leu Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu
            820                 825                 830
ttc aaa gca gtg cgc gaa act atg gtt gac aca tat gag tct tgt gat       2544
Phe Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp
        835                 840                 845
gta ctg gct gat ttc tac gac cag ttc gct gac cag ttg cac gag tct       2592
Val Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser
    850                 855                 860
caa ttg gac aaa atg cca gca ctt ccg gct aaa ggt aac ttg aac ctc       2640
Gln Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu
865                 870                 875                 880
cgt gac atc tta gag tcg gac ttc gcg ttc gcg taa                       2676
Arg Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala
                885                 890


<210>  14
<211>  891
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase protein

<400>  14
Met Gly His His His His His His Met Asn Thr Ile Asn Ile Ala Lys
1               5                   10                  15
Asn Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu
            20                  25                  30
Ala Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu
        35                  40                  45
His Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu
    50                  55                  60
Arg Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro
65                  70                  75                  80
Leu Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp
                85                  90                  95
Phe Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln
            100                 105                 110
Phe Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys
        115                 120                 125
Thr Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala
    130                 135                 140
Val Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly
145                 150                 155                 160
Arg Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu
                165                 170                 175
Gln Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln
            180                 185                 190
Val Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala
        195                 200                 205
Trp Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys
    210                 215                 220
Ile Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln
225                 230                 235                 240
Asn Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro
                245                 250                 255
Glu Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile
            260                 265                 270
Ser Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly
        275                 280                 285
Ile Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu
    290                 295                 300
Val Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr
305                 310                 315                 320
Met Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp
                325                 330                 335
Lys Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp
            340                 345                 350
Lys His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu
        355                 360                 365
Pro Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala
    370                 375                 380
Trp Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys
385                 390                 395                 400
Ser Arg Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe
                405                 410                 415
Ala Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly
            420                 425                 430
Arg Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr
        435                 440                 445
Lys Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly
    450                 455                 460
Tyr Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys
465                 470                 475                 480
Val Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn
                485                 490                 495
Ile Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu
            500                 505                 510
Gln Asp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly
        515                 520                 525
Val Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe
    530                 535                 540
Asp Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp
545                 550                 555                 560
Glu Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln
                565                 570                 575
Asp Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala
            580                 585                 590
Asp Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu
        595                 600                 605
Asn Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu
    610                 615                 620
Ala Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg
625                 630                 635                 640
Ser Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln
                645                 650                 655
Gln Val Leu Glu Asp Thr Ile Gln Pro Ala Ile Asp Ser Gly Lys Gly
            660                 665                 670
Leu Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu
        675                 680                 685
Ile Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met
    690                 695                 700
Asn Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp
705                 710                 715                 720
Lys Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val
                725                 730                 735
Thr Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln
            740                 745                 750
Thr Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr
        755                 760                 765
Ile Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser
    770                 775                 780
Gly Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg
785                 790                 795                 800
Lys Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala
                805                 810                 815
Leu Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu
            820                 825                 830
Phe Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp
        835                 840                 845
Val Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser
    850                 855                 860
Gln Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu
865                 870                 875                 880
Arg Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala
                885                 890


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR and sequence primer

<400>  15
gagcggataa caatttcaca cagg                                              24


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR and sequence primer

<400>  16
gcattggtaa ctgtcagacc aag                                               23


<210>  17
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  17
ggtatggctg tgcaggtcgt aaatcactg                                         29


<210>  18
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  18
cgactggttt gaggaagtga aagctaagcg                                        30


<210>  19
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  19
aggtcaagac tctgagacta tcgaactcgc                                        30


<210>  20
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  20
acaaggctcg caagtctcgc cgtatcagcc                                        30


<210>  21
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  21
tcttgctctg gcatccagca cttctccgcg                                        30


<210>  22
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  22
tggtagctgc ggttgaagca atgaactggc                                        30


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  23
tgtactggct gatttctacg accagttcgc                                        30


<210>  24
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  24
tatttgatgc ctggcagttc cctactctc                                         29


<210>  25
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  25
aagattcgat tccgtacttc tcgtgtgccc                                        30


<210>  26
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  26
gtatcttcca gcacttgttg acggaagccg                                        30


<210>  27
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  27
accaagtgtt ctccagtgga gacttagcg                                         29


<210>  28
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  28
tgtcctcgac cggacaatgc ttccacttgg                                        30


<210>  29
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  29
ccgagtagac ccttagagag catgtcagcc                                        30


<210>  30
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence primer

<400>  30
cccatctcgt aagactcat gctcaagggcc                                        30


<210>  31
<211>  2652
<212>  DNA
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase gene

<400>  31
atg aac acg att aac atc gct aag aac gac ttc tct gac atc gaa ctg         48
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
gct gct atc ccg ttc aac act ctg gct gac cat tac ggt gag cgt tta         96
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
gct cgc gaa cag ttg gcc ctt gag cat gag tct tac gag atg ggt gaa        144
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
gca cgc ttc cgc aag atg ttt gag cgt caa ctt aaa gct ggt gag gtt        192
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
gcg gat aac gct gcc gcc aag cct ctc atc act acc cta ctc cct aag        240
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
atg att gca cgc atc aac gac tgg ttt gag gaa gtg aaa gct aag cgc        288
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
ggc aag cgc ccg aca gcc ttc cag ttc ctg caa gaa atc aag ccg gaa        336
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
gcc gta gcg tac atc acc att aag acc act ctg gct tgc cta acc agt        384
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
gct gac aat aca acc gtt cag gct gta gca agc gca atc ggt cgg gcc        432
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
att gag gac gag gct cgc ttc ggt cgt atc cgt gac ctt gaa gct aag        480
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
cac ttc aag aaa aac gtt gag gaa caa ctc aac aag cgc gta ggg cac        528
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
gtc tac gag aaa gca ttt atg caa gtt gtc gag gct gac atg ctc tct        576
Val Tyr Glu Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
aag ggt cta ctc ggt ggc gag gcg tgg tct tcg tgg cat aag gaa gac        624
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
tct att cat gta gga gta cgc tgc atc gag atg ctc att gag tca acc        672
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
gga atg gtt agc tta cac cgc caa aat gct ggc gta gta ggt caa gac        720
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
tct gag act atc gaa ctc gca cct gaa tac gct gag gct atc gca acc        768
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
cgt gca ggt gcg ctg gct ggc atc tct ccg atg ttc caa cct tgc gta        816
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
gtt cct cct aag ccg tgg act ggc att act ggt ggt ggc tat tgg gct        864
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
aac ggt cgt cgt cct ctg gcg ctg gtg cgt act cac agt aag aaa gca        912
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
ctg atg cgc tac gaa gac gtt tac atg cct gag gtg tac aaa gcg att        960
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
aac att gcg caa aac acc gca tgg aaa atc aac aag aaa gtc cta gcg       1008
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
gtc gcc aac gta atc acc aag tgg aag cat tgt ccg gtc gag gac atc       1056
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
cct gcg att gag cgt gaa gaa ctc ccg atg aaa ccg gaa gac atc gac       1104
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
atg aat cct gag gct ctc acc gcg tgg aaa cgt gct gcc gct gct gtg       1152
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
tac cgc aag gac aag gct cgc aag tct cgc cgt atc agc ctt gag ttc       1200
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
atg ctt gag caa gcc aat aag ttt gct aac cat aag gcc atc tgg ttc       1248
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
cct tac aac atg gac tgg cgc ggt cgt gtt tac gct gtg tca atg ttc       1296
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
aac ccg caa ggt aac gat atg acc aaa gga ctg ctt acg ctg gcg aaa       1344
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
ggt aaa cca atc ggt aag gaa ggt tac tac tgg ctg aaa atc cac ggt       1392
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
gca aac tgt gcg ggt gtc gat aag gtt ccg ttc cct gag cgc atc aag       1440
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
ttc att gag gaa aac cac gag aac atc atg gct tgc gct aag tct cca       1488
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
ctg gag aac act tgg tgg gct gag caa gat tct ccg ttc tgc ttc ctt       1536
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
gcg ttc tgc ttt gag tac gct ggg gta cag cac cac ggc ctg agc tat       1584
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
aac tgc tcc ctt ccg ctg gcg ttt gac ggg tct tgc tct ggc atc cag       1632
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
cac ttc tcc gcg atg ctc cga gat gag gta ggt ggt cgc gcg gtt aac       1680
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
ttg ctt cct agt gaa acc gtt cag gac atc tac ggg att gtt gct aag       1728
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
aaa gtc aac gag att cta caa gca gac gca atc aat ggg acc gat aac       1776
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
gaa gta gtt acc gtg acc gat gag aac act ggt gaa atc tct gag aaa       1824
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
gtc aag ctt ggc act aag gca ctg gct ggt caa tgg ctg gct tac ggt       1872
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
gtt act cgc agt gtg act aag cgt tca gtc atg acg ctg gct tac ggg       1920
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
tcc aaa gag ttc ggc ttc cgt caa caa gtg ctg gaa gat acc att cag       1968
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
cca gct att gat tcc ggc aag ggt ctg atg ttc act cag ccg aat cag       2016
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
gct gct gga tac atg gct aag ctg att tgg gaa tct gtg agc gtg acg       2064
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr
        675                 680                 685
gtg gta gct gcg gtt gaa gca atg aac tgg ctt aag tct gct gct aag       2112
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
ctt ctg gct gct gag gtc aaa gat aag aag act gga gag att ctt cgc       2160
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
aag cgt tgc gct gtg cat tgg gta act cct gat ggt ttc cct gtg tgg       2208
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
cag gaa tac aag aag cct att cag acg cgc ttg aac ctg atg ttc ctc       2256
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
ggt cag ttc cgc tta cag cct acc att aac acc aac aaa gat agc gag       2304
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
att gat gca cac aaa cag gag tct ggt atc gct cct aac ttt gta cac       2352
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
agc caa gac ggt agc cac ctt cgt aag act gta gtg tgg gca cac gag       2400
Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
aag tac gga atc gaa tct ttt gca ctg att cac gac tcc ttc ggt acc       2448
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
att ccg gct gac gct gcg aac ctg ttc aaa gca gtg cgc gaa act atg       2496
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
gtt gac aca tat gag tct tgt gat gta ctg gct gat ttc tac gac cag       2544
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
ttc gct gac cag ttg cac gag tct caa ttg gac aaa atg cca gca ctt       2592
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
ccg gct aaa ggt aac ttg aac ctc cgt gac atc tta gag tcg gac ttc       2640
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
gcg ttc gcg taa                                                       2652
Ala Phe Ala


<210>  32
<211>  883
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase protein

<400>  32
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
Val Tyr Glu Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr
        675                 680                 685
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
Ala Phe Ala


<210>  33
<211>  2652
<212>  DNA
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase gene

<400>  33
atg aac acg att aac atc gct aag aac gac ttc tct gac atc gaa ctg         48
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
gct gct atc ccg ttc aac act ctg gct gac cat tac ggt gag cgt tta         96
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
gct cgc gaa cag ttg gcc ctt gag cat gag tct tac gag atg ggt gaa        144
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
gca cgc ttc cgc aag atg ttt gag cgt caa ctt aaa gct ggt gag gtt        192
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
gcg gat aac gct gcc gcc aag cct ctc atc act acc cta ctc cct aag        240
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
atg att gca cgc atc aac gac tgg ttt gag gaa gtg aaa gct aag cgc        288
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
ggc aag cgc ccg aca gcc ttc cag ttc ctg caa gaa atc aag ccg gaa        336
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
gcc gta gcg tac atc acc att aag acc act ctg gct tgc cta acc agt        384
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
gct gac aat aca acc gtt cag gct gta gca agc gca atc ggt cgg gcc        432
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
att gag gac gag gct cgc ttc ggt cgt atc cgt gac ctt gaa gct aag        480
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
cac ttc aag aaa aac gtt gag gaa caa ctc aac aag cgc gta ggg cac        528
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
gtc tac aag aaa gca ttt atg caa gtt gtc gag gct gac atg ctc tct        576
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
aag ggt cta ctc ggt ggc gag gcg tgg tct tcg tgg cat aag gaa gac        624
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
tct att cat gta gga gta cgc tgc atc gag atg ctc att gag tca acc        672
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
gga atg gtt agc tta cac cgc caa aat gct ggc gta gta ggt caa gac        720
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
tct gag act atc gaa ctc gca cct gaa tac gct gag gct atc gca acc        768
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
cgt gca ggt gcg ctg gct ggc atc tct ccg atg ttc caa cct tgc gta        816
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
gtt cct cct aag ccg tgg act ggc att act ggt ggt ggc tat tgg gct        864
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
aac ggt cgt cgt cct ctg gcg ctg gtg cgt act cac agt aag aaa gca        912
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
ctg atg cgc tac gaa gac gtt tac atg cct gag gtg tac aaa gcg att        960
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
aac att gcg caa aac acc gca tgg aaa atc aac aag aaa gtc cta gcg       1008
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
gtc gcc aac gta atc acc aag tgg aag cat tgt ccg gtc gag gac atc       1056
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
cct gcg att gag cgt gaa gaa ctc ccg atg aaa ccg gaa gac atc gac       1104
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
atg aat cct gag gct ctc acc gcg tgg aaa cgt gct gcc gct gct gtg       1152
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
tac cgc aag gac aag gct cgc aag tct cgc cgt atc agc ctt gag ttc       1200
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
atg ctt gag caa gcc aat aag ttt gct aac cat aag gcc atc tgg ttc       1248
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
cct tac aac atg gac tgg cgc ggt cgt gtt tac gct gtg tca atg ttc       1296
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
aac ccg caa ggt aac gat atg acc aaa gga ctg ctt acg ctg gcg aaa       1344
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
ggt aaa cca atc ggt aag gaa ggt tac tac tgg ctg aaa atc cac ggt       1392
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
gca aac tgt gcg ggt gtc gat aag gtt ccg ttc cct gag cgc atc aag       1440
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
ttc att gag gaa aac cac gag aac atc atg gct tgc gct aag tct cca       1488
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
ctg gag aac act tgg tgg gct gag caa gat tct ccg ttc tgc ttc ctt       1536
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
gcg ttc tgc ttt gag tac gct ggg gta cag cac cac ggc ctg agc tat       1584
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
aac tgc tcc ctt ccg ctg gcg ttt gac ggg tct tgc tct ggc atc cag       1632
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
cac ttc tcc gcg atg ctc cga gat gag gta ggt ggt cgc gcg gtt aac       1680
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
ttg ctt cct agt gaa acc gtt cag gac atc tac ggg att gtt gct aag       1728
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
aaa gtc aac gag att cta caa gca gac gca atc aat ggg acc gat aac       1776
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
gaa gta gtt acc gtg acc gat gag aac act ggt gaa atc tct gag aaa       1824
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
gtc aag ctt ggc act aag gca ctg gct ggt caa tgg ctg gct tac ggt       1872
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
gtt act cgc agt gtg act aag cgt tca gtc atg acg ctg gct tac ggg       1920
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
tcc aaa gag ttc ggc ttc cgt caa caa gtg ctg gaa gat acc att cag       1968
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
cca gct att gat tcc ggc aag ggt ctg atg ttc act cag ccg aat cag       2016
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
gct gct gga tac atg gct aag ctg att tgg gaa tct gtg agc gtg acg       2064
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr
        675                 680                 685
gtg gta gct gcg gtt gaa gca atg aac tgg ctt aag tct gct gct aag       2112
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
ctt ctg gct gct gag gtc aaa gat aag aag act gga gag att ctt cgc       2160
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
aag cgt tgc gct gtg cat tgg gta act cct gat ggt ttc cct gtg tgg       2208
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
cag gaa tac aag aag cct att cag acg cgc ttg aac ctg atg ttc ctc       2256
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
ggt cag ttc cgc tta cag cct acc att aac acc aac aaa gat agc gag       2304
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
att gat gca cac aaa cag gag tct ggt atc gct cct aac ttt gta cac       2352
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
agc cta gac ggt agc cac ctt cgt aag act gta gtg tgg gca cac gag       2400
Ser Leu Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
aag tac gga atc gaa tct ttt gca ctg att cac gac tcc ttc ggt acc       2448
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
att ccg gct gac gct gcg aac ctg ttc aaa gca gtg cgc gaa act atg       2496
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
gtt gac aca tat gag tct tgt gat gta ctg gct gat ttc tac gac cag       2544
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
ttc gct gac cag ttg cac gag tct caa ttg gac aaa atg cca gca ctt       2592
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
ccg gct aaa ggt aac ttg aac ctc cgt gac atc tta gag tcg gac ttc       2640
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
gcg ttc gcg taa                                                       2652
Ala Phe Ala


<210>  34
<211>  883
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase protein

<400>  34
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr
        675                 680                 685
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
Ser Leu Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
Ala Phe Ala


<210>  35
<211>  2652
<212>  DNA
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase gene

<400>  35
atg aac acg att aac atc gct aag aac gac ttc tct gac atc gaa ctg         48
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
gct gct atc ccg ttc aac act ctg gct gac cat tac ggt gag cgt tta         96
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
gct cgc gaa cag ttg gcc ctt gag cat gag tct tac gag atg ggt gaa        144
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
gca cgc ttc cgc aag atg ttt gag cgt caa ctt aaa gct ggt gag gtt        192
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
gcg gat aac gct gcc gcc aag cct ctc atc act acc cta ctc cct aag        240
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
atg att gca cgc atc aac gac tgg ttt gag gaa gtg aaa gct aag cgc        288
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
ggc aag cgc ccg aca gcc ttc cag ttc ctg caa gaa atc aag ccg gaa        336
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
gcc gta gcg tac atc acc att aag acc act ctg gct tgc cta acc agt        384
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
gct gac aat aca acc gtt cag gct gta gca agc gca atc ggt cgg gcc        432
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
att gag gac gag gct cgc ttc ggt cgt atc cgt gac ctt gaa gct aag        480
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
cac ttc aag aaa aac gtt gag gaa caa ctc aac aag cgc gta ggg cac        528
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
gtc tac gag aaa gca ttt atg caa gtt gtc gag gct gac atg ctc tct        576
Val Tyr Glu Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
aag ggt cta ctc ggt ggc gag gcg tgg tct tcg tgg cat aag gaa gac        624
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
tct att cat gta gga gta cgc tgc atc gag atg ctc att gag tca acc        672
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
gga atg gtt agc tta cac cgc caa aat gct ggc gta gta ggt caa gac        720
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
tct gag act atc gaa ctc gca cct gaa tac gct gag gct atc gca acc        768
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
cgt gca ggt gcg ctg gct ggc atc tct ccg atg ttc caa cct tgc gta        816
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
gtt cct cct aag ccg tgg act ggc att act ggt ggt ggc tat tgg gct        864
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
aac ggt cgt cgt cct ctg gcg ctg gtg cgt act cac agt aag aaa gca        912
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
ctg atg cgc tac gaa gac gtt tac atg cct gag gtg tac aaa gcg att        960
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
aac att gcg caa aac acc gca tgg aaa atc aac aag aaa gtc cta gcg       1008
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
gtc gcc aac gta atc acc aag tgg aag cat tgt ccg gtc gag gac atc       1056
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
cct gcg att gag cgt gaa gaa ctc ccg atg aaa ccg gaa gac atc gac       1104
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
atg aat cct gag gct ctc acc gcg tgg aaa cgt gct gcc gct gct gtg       1152
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
tac cgc aag gac aag gct cgc aag tct cgc cgt atc agc ctt gag ttc       1200
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
atg ctt gag caa gcc aat aag ttt gct aac cat aag gcc atc tgg ttc       1248
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
cct tac aac atg gac tgg cgc ggt cgt gtt tac gct gtg tca atg ttc       1296
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
aac ccg caa ggt aac gat atg acc aaa gga ctg ctt acg ctg gcg aaa       1344
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
ggt aaa cca atc ggt aag gaa ggt tac tac tgg ctg aaa atc cac ggt       1392
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
gca aac tgt gcg ggt gtc gat aag gtt ccg ttc cct gag cgc atc aag       1440
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
ttc att gag gaa aac cac gag aac atc atg gct tgc gct aag tct cca       1488
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
ctg gag aac act tgg tgg gct gag caa gat tct ccg ttc tgc ttc ctt       1536
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
gcg ttc tgc ttt gag tac gct ggg gta cag cac cac ggc ctg agc tat       1584
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
aac tgc tcc ctt ccg ctg gcg ttt gac ggg tct tgc tct ggc atc cag       1632
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
cac ttc tcc gcg atg ctc cga gat gag gta ggt ggt cgc gcg gtt aac       1680
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
ttg ctt cct agt gaa acc gtt cag gac atc tac ggg att gtt gct aag       1728
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
aaa gtc aac gag att cta caa gca gac gca atc aat ggg acc gat aac       1776
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
gaa gta gtt acc gtg acc gat gag aac act ggt gaa atc tct gag aaa       1824
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
gtc aag ctt ggc act aag gca ctg gct ggt caa tgg ctg gct tac ggt       1872
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
gtt act cgc agt gtg act aag cgt tca gtc atg acg ctg gct tac ggg       1920
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
tcc aaa gag ttc ggc ttc cgt caa caa gtg ctg gaa gat acc att cag       1968
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
cca gct att gat tcc ggc aag ggt ctg atg ttc act cag ccg aat cag       2016
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
gct gct gga tac atg gct aag ctg att tgg gaa tct gtg agc gtg acg       2064
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr
        675                 680                 685
gtg gta gct gcg gtt gaa gca atg aac tgg ctt aag tct gct gct aag       2112
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
ctt ctg gct gct gag gtc aaa gat aag aag act gga gag att ctt cgc       2160
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
aag cgt tgc gct gtg cat tgg gta act cct gat ggt ttc cct gtg tgg       2208
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
cag gaa tac aag aag cct att cag acg cgc ttg aac ctg atg ttc ctc       2256
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
ggt cag ttc cgc tta cag cct acc att aac acc aac aaa gat agc gag       2304
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
att gat gca cac aaa cag gag tct ggt atc gct cct aac ttt gta cac       2352
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
agc cta gac ggt agc cac ctt cgt aag act gta gtg tgg gca cac gag       2400
Ser Leu Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
aag tac gga atc gaa tct ttt gca ctg att cac gac tcc ttc ggt acc       2448
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
att ccg gct gac gct gcg aac ctg ttc aaa gca gtg cgc gaa act atg       2496
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
gtt gac aca tat gag tct tgt gat gta ctg gct gat ttc tac gac cag       2544
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
ttc gct gac cag ttg cac gag tct caa ttg gac aaa atg cca gca ctt       2592
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
ccg gct aaa ggt aac ttg aac ctc cgt gac atc tta gag tcg gac ttc       2640
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
gcg ttc gcg taa                                                       2652
Ala Phe Ala


<210>  36
<211>  883
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase protein

<400>  36
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
Val Tyr Glu Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr
        675                 680                 685
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
Ser Leu Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
Ala Phe Ala



<210>  37
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  37
gtagggcacg tctacnnkaa agcatttatg caag                                   34


<210>  38
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  38
gtcgatgtct tccggtttca tcggg                                             25


<210>  39
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  39
cttgcataaa tgctttmnng tagacgtgcc ctac                                   34


<210>  40
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  40
ctaactttgt acacagcnnk gacggtagcc accttcg                                37


<210>  41
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  41
cgaaggtggc taccgtcmnn gctgtgtaca aagttag                                37


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR and sequence primer

<400>  42
gtgaccgatg agaacactgg                                                   20

<210>  43
<211>  2652
<212>  DNA
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase

<400>  43
atg aac acg att aac atc gct aag aac gac ttc tct gac atc gaa ctg         48
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
gct gct atc ccg ttc aac act ctg gct gac cat tac ggt gag cgt tta         96
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
gct cgc gaa cag ttg gcc ctt gag cat gag tct tac gag atg ggt gaa        144
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
gca cgc ttc cgc aag atg ttt gag cgt caa ctt aaa gct ggt gag gtt        192
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
gcg gat aac gct gcc gcc aag cct ctc atc act acc cta ctc cct aag        240
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
atg att gca cgc atc aac gac tgg ttt gag gaa gtg aaa gct aag cgc        288
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
ggc aag cgc ccg aca gcc ttc cag ttc ctg caa gaa atc aag ccg gaa        336
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
gcc gta gcg tac atc acc att aag acc act ctg gct tgc cta acc agt        384
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
gct gac aat aca acc gtt cag gct gta gca agc gca atc ggt cgg gcc        432
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
att gag gac gag gct cgc ttc ggt cgt atc cgt gac ctt gaa gct aag        480
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
cac ttc aag aaa aac gtt gag gaa caa ctc aac aag cgc gta ggg cac        528
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
gtc tac gag aaa gca ttt atg caa gtt gtc gag gct gac atg ctc tct        576
Val Tyr Glu Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
aag ggt cta ctc ggt ggc gag gcg tgg tct tcg tgg cat aag gaa gac        624
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
tct att cat gta gga gta cgc tgc atc gag atg ctc att gag tca acc        672
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
gga atg gtt agc tta cac cgc caa aat gct ggc gta gta ggt caa gac        720
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
tct gag act atc gaa ctc gca cct gaa tac gct gag gct atc gca acc        768
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
cgt gca ggt gcg ctg gct ggc atc tct ccg atg ttc caa cct tgc gta        816
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
gtt cct cct aag ccg tgg act ggc att act ggt ggt ggc tat tgg gct        864
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
aac ggt cgt cgt cct ctg gcg ctg gtg cgt act cac agt aag aaa gca        912
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
ctg atg cgc tac gaa gac gtt tac atg cct gag gtg tac aaa gcg att        960
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
aac att gcg caa aac acc gca tgg aaa atc aac aag aaa gtc cta gcg       1008
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
gtc gcc aac gta atc acc aag tgg aag cat tgt ccg gtc gag gac atc       1056
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
cct gcg att gag cgt gaa gaa ctc ccg atg aaa ccg gaa gac atc gac       1104
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
atg aat cct gag gct ctc acc gcg tgg aaa cgt gct gcc gct gct gtg       1152
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
tac cgc aag gac aag gct cgc aag tct cgc cgt atc agc ctt gag ttc       1200
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
atg ctt gag caa gcc aat aag ttt gct aac cat aag gcc atc tgg ttc       1248
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
cct tac aac atg gac tgg cgc ggt cgt gtt tac gct gtg tca atg ttc       1296
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
aac ccg caa ggt aac gat atg acc aaa gga ctg ctt acg ctg gcg aaa       1344
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
ggt aaa cca atc ggt aag gaa ggt tac tac tgg ctg aaa atc cac ggt       1392
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
gca aac tgt gcg ggt gtc gat aag gtt ccg ttc cct gag cgc atc aag       1440
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
ttc att gag gaa aac cac gag aac atc atg gct tgc gct aag tct cca       1488
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
ctg gag aac act tgg tgg gct gag caa gat tct ccg ttc tgc ttc ctt       1536
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
gcg ttc tgc ttt gag tac gct ggg gta cag cac cac ggc ctg agc tat       1584
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
aac tgc tcc ctt ccg ctg gcg ttt gac ggg tct tgc tct ggc atc cag       1632
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
cac ttc tcc gcg atg ctc cga gat gag gta ggt ggt cgc gcg gtt aac       1680
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
ttg ctt cct agt gaa acc gtt cag gac atc tac ggg att gtt gct aag       1728
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
aaa gtc aac gag att cta caa gca gac gca atc aat ggg acc gat aac       1776
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
gaa gta gtt acc gtg acc gat gag aac act ggt gaa atc tct gag aaa       1824
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
gtc aag ctt ggc act aag gca ctg gct ggt caa tgg ctg gct tac ggt       1872
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
gtt act cgc agt gtg act aag cgt tca gtc atg acg ctg gct tac ggg       1920
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
tcc aaa gag ttc ggc ttc cgt caa caa gtg ctg gaa gat acc att cag       1968
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
cca gct att gat tcc ggc aag ggt ctg atg ttc act cag ccg aat cag       2016
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
gct gct gga tac atg gct aag ctg att tgg gaa tct gcg agc gtg acg       2064
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Ala Ser Val Thr
        675                 680                 685
gtg gta gct gcg gtt gaa gca atg aac tgg ctt aag tct gct gct aag       2112
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
ctt ctg gct gct gag gtc aaa gat aag aag act gga gag att ctt cgc       2160
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
aag cgt tgc gct gtg cat tgg gta act cct gat ggt ttc cct gtg tgg       2208
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
cag gaa tac aag aag cct att cag acg cgc ttg aac ctg atg ttc ctc       2256
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
ggt cag ttc cgc tta cag cct acc att aac acc aac aaa gat agc gag       2304
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
att gat gca cac aaa cag gag tct ggt atc gct cct aac ttt gta cac       2352
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
agc cta gac ggt agc cac ctt cgt aag act gta gtg tgg gca cac gag       2400
Ser Leu Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
aag tac gga atc gaa tct ttt gca ctg att cac gac tcc ttc ggt acc       2448
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
att ccg gct gac gct gcg aac ctg ttc aaa gca gtg cgc gaa act atg       2496
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
gtt gac aca tat gag tct tgt gat gta ctg gct gat ttc tac gac cag       2544
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
ttc gct gac cag ttg cac gag tct caa ttg gac aaa atg cca gca ctt       2592
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
ccg gct aaa ggt aac ttg aac ctc cgt gac atc tta gag tcg gac ttc       2640
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
gcg ttc gcg taa                                                       2652
Ala Phe Ala


<210>  44
<211>  883
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase

<400>  44
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
Val Tyr Glu Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Ala Ser Val Thr
        675                 680                 685
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
Ser Leu Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
Ala Phe Ala


<210>  45
<211>  2652
<212>  DNA
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase

<400>  45
atg aac acg att aac atc gct aag aac gac ttc tct gac atc gaa ctg         48
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
gct gct atc ccg ttc aac act ctg gct gac cat tac ggt gag cgt tta         96
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
gct cgc gaa cag ttg gcc ctt gag cat gag tct tac gag atg ggt gaa        144
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
gca cgc ttc cgc aag atg ttt gag cgt caa ctt aaa gct ggt gag gtt        192
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
gcg gat aac gct gcc gcc aag cct ctc atc act acc cta ctc cct aag        240
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
atg att gca cgc atc aac gac tgg ttt gag gaa gtg aaa gct aag cgc        288
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
ggc aag cgc ccg aca gcc ttc cag ttc ctg caa gaa atc aag ccg gaa        336
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
gcc gta gcg tac atc acc att aag acc act ctg gct tgc cta acc agt        384
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
gct gac aat aca acc gtt cag gct gta gca agc gca atc ggt cgg gcc        432
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
att gag gac gag gct cgc ttc ggt cgt atc cgt gac ctt gaa gct aag        480
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
cac ttc aag aaa aac gtt gag gaa caa ctc aac aag cgc gta ggg cac        528
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
gtc tac aag aaa gca ttt atg caa gtt gtc gag gct gac atg ctc tct        576
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
aag ggt cta ctc ggt ggc gag gcg tgg tct tcg tgg cat aag gaa gac        624
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
tct att cat gta gga gta cgc tgc atc gag atg ctc att gag tca acc        672
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
gga atg gtt agc tta cac cgc caa aat gct ggc gta gta ggt caa gac        720
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
tct gag act atc gaa ctc gca cct gaa tac gct gag gct atc gca acc        768
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
cgt gca ggt gcg ctg gct ggc atc tct ccg atg ttc caa cct tgc gta        816
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
gtt cct cct aag ccg tgg act ggc att act ggt ggt ggc tat tgg gct        864
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
aac ggt cgt cgt cct ctg gcg ctg gtg cgt act cac agt aag aaa gca        912
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
ctg atg cgc tac gaa gac gtt tac atg cct gag gtg tac aaa gcg att        960
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
aac att gcg caa aac acc gca tgg aaa atc aac aag aaa gtc cta gcg       1008
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
gtc gcc aac gta atc acc aag tgg aag cat tgt ccg gtc gag gac atc       1056
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
cct gcg att gag cgt gaa gaa ctc ccg atg aaa ccg gaa gac atc gac       1104
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
atg aat cct gag gct ctc acc gcg tgg aaa cgt gct gcc gct gct gtg       1152
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
tac cgc aag gac aag gct cgc aag tct cgc cgt atc agc ctt gag ttc       1200
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
atg ctt gag caa gcc aat aag ttt gct aac cat aag gcc atc tgg ttc       1248
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
cct tac aac atg gac tgg cgc ggt cgt gtt tac gct gtg tca atg ttc       1296
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
aac ccg caa ggt aac gat atg acc aaa gga ctg ctt acg ctg gcg aaa       1344
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
ggt aaa cca atc ggt aag gaa ggt tac tac tgg ctg aaa atc cac ggt       1392
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
gca aac tgt gcg ggt gtc gat aag gtt ccg ttc cct gag cgc atc aag       1440
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
ttc att gag gaa aac cac gag aac atc atg gct tgc gct aag tct cca       1488
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
ctg gag aac act tgg tgg gct gag caa gat tct ccg ttc tgc ttc ctt       1536
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
gcg ttc tgc ttt gag tac gct ggg gta cag cac cac ggc ctg agc tat       1584
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
aac tgc tcc ctt ccg ctg gcg ttt gac ggg tct tgc tct ggc atc cag       1632
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
cac ttc tcc gcg atg ctc cga gat gag gta ggt ggt cgc gcg gtt aac       1680
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
ttg ctt cct agt gaa acc gtt cag gac atc tac ggg att gtt gct aag       1728
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
aaa gtc aac gag att cta caa gca gac gca atc aat ggg acc gat aac       1776
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
gaa gta gtt acc gtg acc gat gag aac act ggt gaa atc tct gag aaa       1824
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
gtc aag ctt ggc act aag gca ctg gct ggt caa tgg ctg gct tac ggt       1872
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
gtt act cgc agt gtg act aag cgt tca gtc atg acg ctg gct tac ggg       1920
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
tcc aaa gag ttc ggc ttc cgt caa caa gtg ctg gaa gat acc att cag       1968
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
cca gct att gat tcc ggc aag ggt ctg atg ttc act cag ccg aat cag       2016
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
gct gct gga tac atg gct aag ctg att tgg gaa tct gcg agc gtg acg       2064
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Ala Ser Val Thr
        675                 680                 685
gtg gta gct gcg gtt gaa gca atg aac tgg ctt aag tct gct gct aag       2112
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
ctt ctg gct gct gag gtc aaa gat aag aag act gga gag att ctt cgc       2160
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
aag cgt tgc gct gtg cat tgg gta act cct gat ggt ttc cct gtg tgg       2208
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
cag gaa tac aag aag cct att cag acg cgc ttg aac ctg atg ttc ctc       2256
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
ggt cag ttc cgc tta cag cct acc att aac acc aac aaa gat agc gag       2304
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
att gat gca cac aaa cag gag tct ggt atc gct cct aac ttt gta cac       2352
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
agc cta gac ggt agc cac ctt cgt aag act gta gtg tgg gca cac gag       2400
Ser Leu Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
aag tac gga atc gaa tct ttt gca ctg att cac gac tcc ttc ggt acc       2448
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
att ccg gct gac gct gcg aac ctg ttc aaa gca gtg cgc gaa act atg       2496
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
gtt gac aca tat gag tct tgt gat gta ctg gct gat ttc tac gac cag       2544
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
ttc gct gac cag ttg cac gag tct caa ttg gac aaa atg cca gca ctt       2592
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
ccg gct aaa ggt aac ttg aac ctc cgt gac atc tta gag tcg gac ttc       2640
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
gcg ttc gcg taa                                                       2652
Ala Phe Ala


<210>  46
<211>  883
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase

<400>  46
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Ala Ser Val Thr
        675                 680                 685
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
Ser Leu Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
Ala Phe Ala


<210>  47
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Scissor oligonucleotide

<400>  47
gatagcggga aagggatcgc                                                   20


<210>  48
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  First primer

<400>  48
aattctaata cgactcacta tagggagact atcggtaaca gtgatgat                    48


<210>  49
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Second primer

<400>  49
ctgactcagg tgctgttgaa                                                   20


<210>  50
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Intercalating fluorescent dye-labeled nucleic acid probe

<400>  50
agcgtagagg caaaagaaag tgggac                                            26


<210>  51
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  51
gatttgggaa tctgcgagcg tgacggtgg                                         29


<210>  52
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  52
gtgacatctt agagtcggac ttcgc                                             25


<210>  53
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  53
ccaccgtcac gctcgcagat tcccaaatc                                         29


<210>  54
<211>  2652
<212>  DNA
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase

<400>  54
atg aac acg att aac atc gct aag aac gac ttc tct gac atc gaa ctg         48
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
gct gct atc ccg ttc aac act ctg gct gac cat tac ggt gag cgt tta         96
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
gct cgc gaa cag ttg gcc ctt gag cat gag tct tac gag atg ggt gaa        144
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
gca cgc ttc cgc aag atg ttt gag cgt caa ctt aaa gct ggt gag gtt        192
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
gcg gat aac gct gcc gcc aag cct ctc atc act acc cta ctc cct aag        240
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
atg att gca cgc atc aac gac tgg ttt gag gaa gtg aaa gct aag cgc        288
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
ggc aag cgc ccg aca gcc ttc cag ttc ctg caa gaa atc aag ccg gaa        336
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
gcc gta gcg tac atc acc att aag acc act ctg gct tgc cta acc agt        384
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
gct gac aat aca acc gtt cag gct gta gca agc gca atc ggt cgg gcc        432
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
att gag gac gag gct cgc ttc ggt cgt atc cgt gac ctt gaa gct aag        480
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
cac ttc aag aaa aac gtt gag gaa caa ctc aac aag cgc gta ggg cac        528
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
gtc tac aag aaa gca ttt atg caa gtt gtc gag gct gac atg ctc tct        576
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
aag ggt cta ctc ggt ggc gag gcg tgg tct tcg tgg cat aag gaa gac        624
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
tct att cat gta gga gta cgc tgc atc gag atg ctc att gag tca acc        672
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
gga atg gtt agc tta cac cgc caa aat gct ggc gta gta ggt caa gac        720
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
tct gag act atc gaa ctc gca cct gaa tac gct gag gct atc gca acc        768
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
cgt gca ggt gcg ctg gct ggc atc tct ccg atg ttc caa cct tgc gta        816
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
gtt cct cct aag ccg tgg act ggc att act ggt ggt ggc tat tgg gct        864
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
aac ggt cgt cgt cct ctg gcg ctg gtg cgt act cac agt aag aaa gca        912
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
ctg atg cgc tac gaa gac gtt tac atg cct gag gtg tac aaa gcg att        960
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
aac att gcg caa aac acc gca tgg aaa atc aac aag aaa gtc cta gcg       1008
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
gtc gcc aac gta atc acc aag tgg aag cat tgt ccg gtc gag gac atc       1056
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
cct gcg att gag cgt gaa gaa ctc ccg atg aaa ccg gaa gac atc gac       1104
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
atg aat cct gag gct ctc acc gcg tgg aaa cgt gct gcc gct gct gtg       1152
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
tac cgc aag gac aag gct cgc aag tct cgc cgt atc agc ctt gag ttc       1200
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
atg ctt gag caa gcc aat aag ttt gct aac cat aag gcc atc tgg ttc       1248
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
cct tac aac atg gac tgg cgc ggt cgt gtt tac gct gtg tca atg ttc       1296
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
aac ccg caa ggt aac gat atg acc aaa gga ctg ctt acg ctg gcg aaa       1344
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
ggt aaa cca atc ggt aag gaa ggt tac tac tgg ctg aaa atc cac ggt       1392
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
gca aac tgt gcg ggt gtc gat aag gtt ccg ttc cct gag cgc atc aag       1440
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
ttc att gag gaa aac cac gag aac atc atg gct tgc gct aag tct cca       1488
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
ctg gag aac act tgg tgg gct gag caa gat tct ccg ttc tgc ttc ctt       1536
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
gcg ttc tgc ttt gag tac gct ggg gta cag cac cac ggc ctg agc tat       1584
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
aac tgc tcc ctt ccg ctg gcg ttt gac ggg tct tgc tct ggc atc cag       1632
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
cac ttc tcc gcg atg ctc cga gat gag gta ggt ggt cgc gcg gtt aac       1680
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
ttg ctt cct agt gaa acc gtt cag gac atc tac ggg att gtt gct aag       1728
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
aaa gtc aac gag att cta caa gca gac gca atc aat ggg acc gat aac       1776
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
gaa gta gtt acc gtg acc gat gag aac act ggt gaa atc tct gag aaa       1824
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
gtc aag ctt ggc act aag gca ctg gct ggt caa tgg ctg gct tac ggt       1872
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
gtt act cgc agt gtg act aag cgt tca gtc atg acg ctg gct tac ggg       1920
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
tcc aaa gag ttc ggc ttc cgt caa caa gtg ctg gaa gat acc att cag       1968
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
cca gct att gat tcc ggc aag ggt ctg atg ttc act cag ccg aat cag       2016
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
gct gct gga tac atg gct aag ctg att tgg gaa tct gcg agc gtg acg       2064
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Ala Ser Val Thr
        675                 680                 685
gtg gta gct gcg gtt gaa gca atg aac tgg ctt aag tct gct gct aag       2112
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
ctt ctg gct gct gag gtc aaa gat aag aag act gga gag att ctt cgc       2160
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
aag cgt tgc gct gtg cat tgg gta act cct gat ggt ttc cct gtg tgg       2208
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
cag gaa tac aag aag cct att cag acg cgc ttg aac ctg atg ttc ctc       2256
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
ggt cag ttc cgc tta cag cct acc att aac acc aac aaa gat agc gag       2304
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
att gat gca cac aaa cag gag tct ggt atc gct cct aac ttt gta cac       2352
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
agc caa gac ggt agc cac ctt cgt aag act gta gtg tgg gca cac gag       2400
Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
aag tac gga atc gaa tct ttt gca ctg att cac gac tcc ttc ggt acc       2448
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
att ccg gct gac gct gcg aac ctg ttc aaa gca gtg cgc gaa act atg       2496
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
gtt gac aca tat gag tct tgt gat gta ctg gct gat ttc tac gac cag       2544
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
ttc gct gac cag ttg cac gag tct caa ttg gac aaa atg cca gca ctt       2592
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
ccg gct aaa ggt aac ttg aac ctc cgt gac atc tta gag tcg gac ttc       2640
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
gcg ttc gcg taa                                                       2652
Ala Phe Ala


<210>  55
<211>  883
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase

<400>  55
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Ala Ser Val Thr
        675                 680                 685
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
Ala Phe Ala


<210>  56
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  56
ctttgtacac agcatggacg gtagccac                                          28


<210>  57
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  57
gtggctaccg tccatgctgt gtacaaag                                          28


<210>  58
<211>  2652
<212>  DNA
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase

<400>  58
atg aac acg att aac atc gct aag aac gac ttc tct gac atc gaa ctg         48
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
gct gct atc ccg ttc aac act ctg gct gac cat tac ggt gag cgt tta         96
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
gct cgc gaa cag ttg gcc ctt gag cat gag tct tac gag atg ggt gaa        144
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
gca cgc ttc cgc aag atg ttt gag cgt caa ctt aaa gct ggt gag gtt        192
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
gcg gat aac gct gcc gcc aag cct ctc atc act acc cta ctc cct aag        240
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
atg att gca cgc atc aac gac tgg ttt gag gaa gtg aaa gct aag cgc        288
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
ggc aag cgc ccg aca gcc ttc cag ttc ctg caa gaa atc aag ccg gaa        336
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
gcc gta gcg tac atc acc att aag acc act ctg gct tgc cta acc agt        384
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
gct gac aat aca acc gtt cag gct gta gca agc gca atc ggt cgg gcc        432
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
att gag gac gag gct cgc ttc ggt cgt atc cgt gac ctt gaa gct aag        480
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
cac ttc aag aaa aac gtt gag gaa caa ctc aac aag cgc gta ggg cac        528
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
gtc tac aag aaa gca ttt atg caa gtt gtc gag gct gac atg ctc tct        576
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
aag ggt cta ctc ggt ggc gag gcg tgg tct tcg tgg cat aag gaa gac        624
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
tct att cat gta gga gta cgc tgc atc gag atg ctc att gag tca acc        672
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
gga atg gtt agc tta cac cgc caa aat gct ggc gta gta ggt caa gac        720
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
tct gag act atc gaa ctc gca cct gaa tac gct gag gct atc gca acc        768
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
cgt gca ggt gcg ctg gct ggc atc tct ccg atg ttc caa cct tgc gta        816
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
gtt cct cct aag ccg tgg act ggc att act ggt ggt ggc tat tgg gct        864
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
aac ggt cgt cgt cct ctg gcg ctg gtg cgt act cac agt aag aaa gca        912
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
ctg atg cgc tac gaa gac gtt tac atg cct gag gtg tac aaa gcg att        960
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
aac att gcg caa aac acc gca tgg aaa atc aac aag aaa gtc cta gcg       1008
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
gtc gcc aac gta atc acc aag tgg aag cat tgt ccg gtc gag gac atc       1056
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
cct gcg att gag cgt gaa gaa ctc ccg atg aaa ccg gaa gac atc gac       1104
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
atg aat cct gag gct ctc acc gcg tgg aaa cgt gct gcc gct gct gtg       1152
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
tac cgc aag gac aag gct cgc aag tct cgc cgt atc agc ctt gag ttc       1200
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
atg ctt gag caa gcc aat aag ttt gct aac cat aag gcc atc tgg ttc       1248
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
cct tac aac atg gac tgg cgc ggt cgt gtt tac gct gtg tca atg ttc       1296
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
aac ccg caa ggt aac gat atg acc aaa gga ctg ctt acg ctg gcg aaa       1344
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
ggt aaa cca atc ggt aag gaa ggt tac tac tgg ctg aaa atc cac ggt       1392
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
gca aac tgt gcg ggt gtc gat aag gtt ccg ttc cct gag cgc atc aag       1440
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
ttc att gag gaa aac cac gag aac atc atg gct tgc gct aag tct cca       1488
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
ctg gag aac act tgg tgg gct gag caa gat tct ccg ttc tgc ttc ctt       1536
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
gcg ttc tgc ttt gag tac gct ggg gta cag cac cac ggc ctg agc tat       1584
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
aac tgc tcc ctt ccg ctg gcg ttt gac ggg tct tgc tct ggc atc cag       1632
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
cac ttc tcc gcg atg ctc cga gat gag gta ggt ggt cgc gcg gtt aac       1680
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
ttg ctt cct agt gaa acc gtt cag gac atc tac ggg att gtt gct aag       1728
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
aaa gtc aac gag att cta caa gca gac gca atc aat ggg acc gat aac       1776
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
gaa gta gtt acc gtg acc gat gag aac act ggt gaa atc tct gag aaa       1824
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
gtc aag ctt ggc act aag gca ctg gct ggt caa tgg ctg gct tac ggt       1872
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
gtt act cgc agt gtg act aag cgt tca gtc atg acg ctg gct tac ggg       1920
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
tcc aaa gag ttc ggc ttc cgt caa caa gtg ctg gaa gat acc att cag       1968
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
cca gct att gat tcc ggc aag ggt ctg atg ttc act cag ccg aat cag       2016
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
gct gct gga tac atg gct aag ctg att tgg gaa tct gcg agc gtg acg       2064
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Ala Ser Val Thr
        675                 680                 685
gtg gta gct gcg gtt gaa gca atg aac tgg ctt aag tct gct gct aag       2112
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
ctt ctg gct gct gag gtc aaa gat aag aag act gga gag att ctt cgc       2160
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
aag cgt tgc gct gtg cat tgg gta act cct gat ggt ttc cct gtg tgg       2208
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
cag gaa tac aag aag cct att cag acg cgc ttg aac ctg atg ttc ctc       2256
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
ggt cag ttc cgc tta cag cct acc att aac acc aac aaa gat agc gag       2304
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
att gat gca cac aaa cag gag tct ggt atc gct cct aac ttt gta cac       2352
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
agc atg gac ggt agc cac ctt cgt aag act gta gtg tgg gca cac gag       2400
Ser Met Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
aag tac gga atc gaa tct ttt gca ctg att cac gac tcc ttc ggt acc       2448
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
att ccg gct gac gct gcg aac ctg ttc aaa gca gtg cgc gaa act atg       2496
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
gtt gac aca tat gag tct tgt gat gta ctg gct gat ttc tac gac cag       2544
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
ttc gct gac cag ttg cac gag tct caa ttg gac aaa atg cca gca ctt       2592
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
ccg gct aaa ggt aac ttg aac ctc cgt gac atc tta gag tcg gac ttc       2640
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
gcg ttc gcg taa                                                       2652
Ala Phe Ala


<210>  59
<211>  883
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7 RNA polymerase

<400>  59
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
1               5                   10                  15
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Ala Ser Val Thr
        675                 680                 685
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780
Ser Met Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880
Ala Phe Ala
