
                                SEQUENCE LISTING

<110> Mayo Foundation for Medical Education and Research

<120> ANTI-PYK2 ANTIBODIES
  

<130> 07039-0866WO1

<150> US 61/056,727       
<151> 2008-05-28  

<160> 5

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 246
<212> PRT
<213> Artificial Sequence

<220> 
<223> Single chain antibody

<400> 1
Met Glu Val Lys Leu Gln Glu Ser Gly Pro Glu Leu Lys Lys Pro Gly
 1               5                  10                  15      
Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp
            20                  25                  30          
Tyr Ser Met His Trp Val Met Gln Ser Pro Gly Lys Gly Leu Lys Trp
        35                  40                  45              
Met Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Arg Tyr Val Asp Asp
    50                  55                  60                  
Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala
65                  70                  75                  80  
Tyr Leu Gln Ile Ile Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe
                85                  90                  95      
Cys Ala Arg Trp Asp His Gly His Gly Gly Phe Thr Tyr Trp Gly Gln
            100                 105                 110         
Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly
        115                 120                 125             
Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Thr Thr Ala
    130                 135                 140                 
Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Met Ser Cys Arg Ala
145                 150                 155                 160 
Thr Glu Ser Val Asp Ser Tyr Gly Lys Ser Phe Met Tyr Trp Phe Gln
                165                 170                 175     
Gln Arg Ala Gly Gln Pro Pro Lys Leu Leu Ile Tyr Leu Ala Ser Asn
            180                 185                 190         
Leu Glu Ser Val Val Pro Pro Arg Phe Ser Gly Ser Gly Ser Arg Thr
        195                 200                 205             
Asp Phe Ser Leu Thr Ile Asp Pro Val Glu Ala Asp Asp Ala Ala Thr
    210                 215                 220                 
Tyr Tyr Cys Gln Gln Asn Asn Glu Asp Pro Phe Thr Phe Gly Ser Gly
225                 230                 235                 240 
Thr Lys Leu Glu Ile Lys
                245     


<210> 2
<211> 744
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleic acid encoding single chain antibody

<400> 2
atggaggtaa agctgcagga gtctggacct gagctgaaga agcctggaga gacagtcaaa 60
atctcctgca aggcctctgg ttataccttc acagactatt caatgcactg ggtgatgcag 120
tctccaggaa agggtttaaa gtggatgggc tggataaaca ctgagactgg tgagcctaga 180
tatgttgatg acttcaaggg gcggtttgcc ttctctttgg aaacctctgc cagcactgcc 240
tatttgcaga tcatcaatct caaaaatgag gacacggcta catatttctg cgctagatgg 300
gaccacggcc acggggggtt tacttactgg ggccaaggga ctctggtcac tgtctctgca 360
ggtggcggtg gctcgggcgg tggtgggtcg ggtggcggcg gatcggatat tgtgctgaca 420
caaactacag cttctttggc tgtgtctcta gggcagaggg ccaccatgtc ctgcagagcc 480
actgaaagtg ttgatagtta tggcaaaagt tttatgtact ggttccagca gagagcagga 540
cagccaccca aactcctcat ctaccttgca tccaacctag aatctgtggt ccctcccagg 600
ttcagtggca gtgggtctag gacagacttc tccctcacca ttgatcctgt ggaggctgat 660
gatgctgcaa cctattactg tcaacaaaat aatgaggatc cattcacgtt cggctcgggg 720
acaaagttgg aaataaaact cgag                                        744

<210> 3
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> Linker

<400> 3
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
 1               5                  10                  15  


<210> 4
<211> 5819
<212> DNA
<213> Artificial Sequence

<220> 
<223> Vector pc12A10scFv 3X HA

<400> 4
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180
ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240
gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300
tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360
cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420
attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480
atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540
atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600
tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660
actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720
aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780
gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840
ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900
taagcttacc atggaggtaa agctgcagga gtctggacct gagctgaaga agcctggaga 960
gacagtcaag atctcctgca aggcctctgg ttataccttc acagactatt caatgcactg 1020
ggtgatgcag tctccaggaa agggtttaaa gtggatgggc tggataaaca ctgagactgg 1080
tgagcctaga tatgttgatg acttcaaggg gcggtttgcc ttctctttgg aaacctctgc 1140
cagcactgcc tatttgcaga tcatcaatct caaaaatgag gacacggcta catatttctg 1200
cgctagatgg gaccacggcc acggggggtt tacttactgg ggccaaggga ctctggtcac 1260
tgtctctgca gccggtggcg gtggctcggg cggtggtggg tcgggtggcg gcggatcgga 1320
tattgtgctg acacaaacta cagcttcttt ggctgtgtct ctagggcaga gggccaccat 1380
gtcctgcaga gccactgaaa gtgttgatag ttatggcaaa agttttatgt actggttcca 1440
gcagagagca ggacagccac ccaaactcct catctacctt gcatccaacc tagaatctgt 1500
ggtccctccc aggttcagtg gcagtgggtc taggacagac ttctccctca ccattgatcc 1560
tgtggaggct gatgatgctg caacctatta ctgtcaacaa aataatgagg atccattcac 1620
gttcggctcg gggacaaagt tggaaataaa actcgaggga tcttacccat acgatgttcc 1680
tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt 1740
tccagattac gcttaggata aacccgctga tcagcctcga ctgtgccttc tagttgccag 1800
ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 1860
gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 1920
ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 1980
gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 2040
gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 2100
agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 2160
tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 2220
ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 2280
cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 2340
tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 2400
tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 2460
caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 2520
caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 2580
gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2640
cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 2700
cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 2760
ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 2820
aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta 2880
atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc 2940
caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag 3000
catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat 3060
cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct 3120
gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga 3180
gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc 3240
tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga 3300
attgctgccc tctggttatg tgtgggaggg ctaagcactt cgtggccgag gagcaggact 3360
gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 3420
tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 3480
tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 3540
caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 3600
tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 3660
ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 3720
ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 3780
cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 3840
tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 3900
ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 3960
taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 4020
agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 4080
cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 4140
tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 4200
tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 4260
gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 4320
acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 4380
acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 4440
cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 4500
gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 4560
gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 4620
agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 4680
ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 4740
ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 4800
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 4860
tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 4920
gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 4980
ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 5040
caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 5100
cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 5160
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 5220
cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 5280
agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 5340
tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 5400
agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 5460
atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 5520
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 5580
cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 5640
caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 5700
attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 5760
agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc  5819



<210> 5
<211> 37
<212> PRT
<213> Artificial Sequence

<220> 
<223> 3X HA epitope

<400> 5
Gly Ser Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Tyr Pro Tyr Asp
 1               5                  10                  15      
Val Pro Asp Tyr Ala Gly Ser Tyr Pro Tyr Asp Val Pro Asp Tyr Ala
            20                  25                  30          
Ala Gln Cys Gly Arg
        35          

