                         SEQUENCE LISTING

<110>  GENZYME CORPORATION
       O'Riordan, Catherine
       Brondyk, William
 
<120>  METHODS TO PRODUCE ROD-DERIVED CONE VIABILITY FACTOR

<130>  5440PCT

<140>  PCT/US2009
<141>  2009-04-15

<150>  US 61/045,077
<151>  2008-04-15

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid linker

<400>  1

Met Pro Leu Glu Ser Gly Leu Ser Ser Glu Asp Ser Ala Ser Ser Glu 
1               5                   10                  15      


Ser Phe Ala 
            


<210>  2
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage marker

<400>  2

Lys Arg Ile Lys Arg 
1               5   


<210>  3
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  3

Arg Xaa Lys Arg Arg 
1               5   


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage marker


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  4

Xaa Arg Xaa Lys Arg Arg 
1               5       


<210>  5
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  5

Glu Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys 
1               5                   10          


<210>  6
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GUSB signal peptide-furin-RdCVF (LF)

<400>  6

Met Ala Arg Gly Ser Ala Val Ala Trp Ala Ala Leu Gly Pro Leu Leu 
1               5                   10                  15      


Trp Gly Cys Ala Leu Gly Leu Gln Met Pro Leu Glu Ser Gly Leu Ser 
            20                  25                  30          


Ser Glu Asp Ser Ala Ser Ser Glu Ser Phe Ala Lys Arg Ile Lys Arg 
        35                  40                  45              


Met Ala Ser Leu Phe Ser Gly Arg Ile Leu Ile Arg Asn Asn Ser Asp 
    50                  55                  60                  


Gln Asp Glu Leu Asp Thr Glu Ala Glu Val Ser Arg Arg Leu Glu Asn 
65                  70                  75                  80  


Arg Leu Val Leu Leu Phe Phe Gly Ala Gly Ala Cys Pro Gln Cys Gln 
                85                  90                  95      


Ala Phe Val Pro Ile Leu Lys Asp Phe Phe Val Arg Leu Thr Asp Glu 
            100                 105                 110         


Phe Tyr Val Leu Arg Ala Ala Gln Leu Ala Leu Val Tyr Val Ser Gln 
        115                 120                 125             


Asp Ser Thr Glu Glu Gln Gln Asp Leu Phe Leu Lys Asp Met Pro Lys 
    130                 135                 140                 


Lys Trp Leu Phe Leu Pro Phe Glu Asp Asp Leu Arg Arg Asp Leu Gly 
145                 150                 155                 160 


Arg Gln Phe Ser Val Glu Arg Leu Pro Ala Val Val Val Leu Lys Pro 
                165                 170                 175     


Asp Gly Asp Val Leu Thr Arg Asp Gly Ala Asp Glu Ile Gln Arg Leu 
            180                 185                 190         


Gly Thr Ala Cys Phe Ala Asn Trp Gln Glu Ala Ala Glu Val Leu Asp 
        195                 200                 205             


Arg Asn Phe Gln Leu Pro Glu Asp Leu Glu Asp Gln Glu Pro Arg Ser 
    210                 215                 220                 


Leu Thr Glu Cys Leu Arg Arg His Lys Tyr Arg Val Glu Lys Ala Ala 
225                 230                 235                 240 


Arg Gly Gly Arg Asp Pro Gly Gly Gly Gly Gly Glu Glu Gly Gly Ala 
                245                 250                 255     


Gly Gly Leu Phe 
            260 


<210>  7
<211>  1101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GUSB signal peptide-furin-RdCVF(LF)

<400>  7
gaattcctcc ggggaccaca cgccgcgctg tccccagcac ttaccatggc ccgggggtcg       60

gcggttgcct gggcggcgct cgggccgttg ttgtggggct gcgcgctggg gctgcagatg      120

cccctcgagt ccggcttgct gtcctccgag gactccgcca gctccgagag cttcgccaag      180

cgcatcaagc gcatggcctc cctgttctct ggccgcatcc tgatccgcaa caatagcgac      240

caggacgagc tggatacgga ggctgaggtc agtcgcaggc tggagaaccg gctggtgctg      300

ctgttctttg gtgctggggc ttgtccacag tgccaggcct tcgtgcccat cctcaaggac      360

ttcttcgtgc ggctcacaga tgagttctat gtactgcggg cggctcagct ggccctggtg      420

tacgtgtccc aggactccac ggaggagcag caggacctgt tcctcaagga catgccaaag      480

aaatggcttt tcctgccctt tgaggatgat ctgaggaggg acctcgggcg ccagttctca      540

gtggagcgcc tgccggcggt cgtggtgctc aagccggacg gggacgtgct cactcgcgac      600

ggcgccgacg agatccagcg cctgggcacc gcctgcttcg ccaactggca ggaggcggcc      660

gaggtgctgg accgcaactt ccagctgcca gaggacctgg aggaccagga gccacggagc      720

ctcaccgagt gcctgcgccg ccacaagtac cgcgtggaaa aggcggcgcg aggcgggcgc      780

gaccccgggg gagggggtgg ggaggagggc ggggccgggg ggctgttctg acccgctagg      840

gtggaggaga ggagtggggt ttgttgatga acctccaccc ccaccccacc cccgcacgcc      900

tgtaatccca gcacttgggg aggccaaggc gggaggatcg cttgagccca gaggttcgag      960

atcaacctgg gcaagagagt gagaccctga ctctacgaaa attaaaagtt agcccggtgt     1020

ggtggcgcgc acctgtggct tagctaccct gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aaaaaaaaaa aaaaagcttc c                                               1101


<210>  8
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BDNF signal peptide-furin-RdCVF(LF)

<400>  8

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Pro Leu Glu Ser Gly Leu Ser Ser Glu Asp Ser 
            20                  25                  30          


Ala Ser Ser Glu Ser Phe Ala Lys Arg Ile Lys Arg Met Ala Ser Leu 
        35                  40                  45              


Phe Ser Gly Arg Ile Leu Ile Arg Asn Asn Ser Asp Gln Asp Glu Leu 
    50                  55                  60                  


Asp Thr Glu Ala Glu Val Ser Arg Arg Leu Glu Asn Arg Leu Val Leu 
65                  70                  75                  80  


Leu Phe Phe Gly Ala Gly Ala Cys Pro Gln Cys Gln Ala Phe Val Pro 
                85                  90                  95      


Ile Leu Lys Asp Phe Phe Val Arg Leu Thr Asp Glu Phe Tyr Val Leu 
            100                 105                 110         


Arg Ala Ala Gln Leu Ala Leu Val Tyr Val Ser Gln Asp Ser Thr Glu 
        115                 120                 125             


Glu Gln Gln Asp Leu Phe Leu Lys Asp Met Pro Lys Lys Trp Leu Phe 
    130                 135                 140                 


Leu Pro Phe Glu Asp Asp Leu Arg Arg Asp Leu Gly Arg Gln Phe Ser 
145                 150                 155                 160 


Val Glu Arg Leu Pro Ala Val Val Val Leu Lys Pro Asp Gly Asp Val 
                165                 170                 175     


Leu Thr Arg Asp Gly Ala Asp Glu Ile Gln Arg Leu Thr Ala Cys Phe 
            180                 185                 190         


Ala Asn Trp Gln Glu Ala Ala Glu Val Leu Asp Arg Asn Phe Gln Leu 
        195                 200                 205             


Pro Glu Asp Leu Glu Asp Gln Glu Pro Arg Ser Leu Thr Glu Cys Leu 
    210                 215                 220                 


Arg Arg His Lys Tyr Arg Val Glu Lys Ala Ala Arg Gly Gly Arg Asp 
225                 230                 235                 240 


Pro Gly Gly Gly Gly Gly Glu Glu Gly Gly Ala Gly Gly Leu Phe 
                245                 250                 255 


<210>  9
<211>  1085
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BDNF signal peptide-furin-RdCVF(LF)

<400>  9
gaattcctcc ggggaccaca cgccgcgctg tccccagcac ttaccatgac catccttttc       60

cttactatgg ttatttcata ctttggttgc atgaaggctg cccccatgcc cctcgagtcc      120

ggcctgtcct ccgaggactc cgccagctcc gagagcttcg ccaagcgcat caagcgcatg      180

gcctccctgt tctctggccg catcctgatc cgcaacaata gcgaccagga cgagctggat      240

acggaggctg aggtcagtcg caggctggag aaccggctgg tgctgctgtt ctttggtgct      300

ggggcttgtc cacagtgcca ggccttcgtg cccatcctca aggacttctt cgtgcggctc      360

acagatgagt tctatgtact gcgggcggct cagctggccc tggtgtacgt gtcccaggac      420

tccacggagg agcagcagga cctgttcctc aaggacatgc caaagaaatg gcttttcctg      480

ccctttgagg atgatctgag gagggacctc gggcgccagt tctcagtgga gcgcctgccg      540

gcggtcgtgg tgctcaagcc ggacggggac gtgctcactc gcgacggcgc cgacgagatc      600

cagcgcctgg gcaccgcctg cttcgccaac tggcaggagg cggccgaggt gctggaccgc      660

aacttccagc tgccagagga cctggaggac caggagccac ggagcctcac cgagtgcctg      720

cgccgccaca agtaccgcgt ggaaaaggcg gcgcgaggcg ggcgcgaccc cgggggaggg      780

ggtggggagg agggcggggc cggggggctg ttctgacccg ctagggtgga ggagaggagt      840

ggggtttgtt gatgaacctc cacccccacc ccacccccgc acgcctgtaa tcccagcact      900

tggggaggcc aaggcgggag gatcgcttga gcccagaggt tcgagatcaa cctgggcaag      960

agagtgagac cctgactcta cgaaaattaa aagttagccc ggtgtggtgg cgcgcacctg     1020

tggcttagct accctgaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

gcttg                                                                 1085


<210>  10
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IGF-1 signal peptide-furin-RdCVF(LF)

<400>  10

Met Gly Lys Ile Ser Ser Leu Pro Thr Gln Leu Phe Lys Cys Cys Phe 
1               5                   10                  15      


Cys Asp Phe Leu Lys Val Lys Met His Thr Met Ser Ser Ser His Leu 
            20                  25                  30          


Phe Tyr Leu Ala Leu Cys Leu Leu Thr Phe Thr Ser Ser Ala Thr Ala 
        35                  40                  45              


Gly Pro Met Pro Leu Glu Ser Gly Leu Ser Ser Glu Asp Ser Ala Ser 
    50                  55                  60                  


Ser Glu Ser Phe Ala Lys Arg Ile Lys Arg Met Ala Ser Leu Phe Ser 
65                  70                  75                  80  


Gly Arg Ile Leu Ile Arg Asn Asn Ser Asp Gln Asp Glu Leu Asp Thr 
                85                  90                  95      


Glu Ala Glu Val Ser Arg Arg Leu Glu Asn Arg Leu Val Leu Leu Phe 
            100                 105                 110         


Phe Gly Ala Gly Ala Cys Pro Gln Cys Gln Ala Phe Val Pro Ile Leu 
        115                 120                 125             


Lys Asp Phe Phe Val Arg Leu Thr Asp Glu Phe Tyr Val Leu Arg Ala 
    130                 135                 140                 


Ala Gln Leu Ala Leu Val Tyr Val Ser Gln Asp Ser Thr Glu Glu Gln 
145                 150                 155                 160 


Gln Asp Leu Phe Leu Lys Asp Met Pro Lys Lys Trp Leu Phe Leu Pro 
                165                 170                 175     


Phe Glu Asp Asp Leu Arg Arg Asp Leu Gly Arg Gln Phe Ser Val Glu 
            180                 185                 190         


Arg Leu Pro Ala Val Val Val Leu Lys Pro Asp Gly Asp Val Leu Thr 
        195                 200                 205             


Arg Asp Gly Ala Asp Glu Ile Gln Arg Leu Gly Thr Ala Cys Phe Ala 
    210                 215                 220                 


Asn Trp Gln Glu Ala Ala Glu Val Leu Asp Arg Asn Phe Gln Leu Pro 
225                 230                 235                 240 


Glu Asp Leu Glu Asp Gln Glu Pro Arg Ser Leu Thr Glu Cys Leu Arg 
                245                 250                 255     


Arg His Lys Tyr Arg Val Glu Lys Ala Ala Arg Gly Gly Arg Asp Pro 
            260                 265                 270         


Gly Gly Gly Gly Gly Glu Glu Gly Gly Ala Gly Gly Leu Phe 
        275                 280                 285     


<210>  11
<211>  1178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IGF-1 signal peptide-furin-RdCVF(LF)

<400>  11
gaattcctcc ggggaccaca cgccgcgctg tccccagcac ttaccatggg aaaaatcagc       60

agtcttccaa cccaattatt taagtgctgc ttttgtgatt tcttgaaggt gaagatgcac      120

accatgtcct cctcgcatct cttctacctg gcgctgtgcc tgctcacctt caccagctct      180

gccacggctg gaccggagat gcccctcgag tccggcctgt cctccgagga ctccgccagc      240

tccgagagct tcgccaagcg catcaagcgc atggcctccc tgttctctgg ccgcatcctg      300

atccgcaaca atagcgacca ggacgagctg gatacggagg ctgaggtcag tcgcaggctg      360

gagaaccggc tggtgctgct gttctttggt gctggggctt gtccacagtg ccaggccttc      420

gtgcccatcc tcaaggactt cttcgtgcgg ctcacagatg agttctatgt actgcgggcg      480

gctcagctgg ccctggtgta cgtgtcccag gactccacgg aggagcagca ggacctgttc      540

ctcaaggaca tgccaaagaa atggcttttc ctgccctttg aggatgatct gaggagggac      600

ctcgggcgcc agttctcagt ggagcgcctg ccggcggtcg tggtgctcaa gccggacggg      660

gacgtgctca ctcgcgacgg cgccgacgag atccagcgcc tgggcaccgc ctgcttcgcc      720

aactggcagg aggcggccga ggtgctggac cgcaacttcc agctgccaga ggacctggag      780

gaccaggagc cacggagcct caccgagtgc ctgcgccgcc acaagtaccg cgtggaaaag      840

gcggcgcgag gcgggcgcga ccccggggga gggggtgggg aggagggcgg ggccgggggg      900

ctgttctgac ccgctagggt ggaggagagg agtggggttt gttgatgaac ctccaccccc      960

accccacccc cgcacgcctg taatcccagc acttggggag gccaaggcgg gaggatcgct     1020

tgagcccaga ggttcgagat caacctgggc aagagagtga gaccctgact ctacgaaaat     1080

taaaagttag cccggtgtgg tggcgcgcac ctgtggctta gctaccctga aaaaaaaaaa     1140

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaagcttc                             1178


<210>  12
<211>  157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GUSB signal peptide-furin-RdCVF(SF)

<400>  12

Met Ala Arg Gly Ser Ala Val Ala Trp Ala Ala Leu Gly Pro Leu Leu 
1               5                   10                  15      


Trp Gly Cys Ala Leu Gly Leu Gln Met Pro Leu Glu Ser Gly Leu Ser 
            20                  25                  30          


Ser Glu Asp Ser Ala Ser Ser Glu Ser Phe Ala Lys Arg Ile Lys Arg 
        35                  40                  45              


Met Ala Ser Leu Phe Ser Gly Arg Ile Leu Ile Arg Asn Asn Ser Asp 
    50                  55                  60                  


Gln Asp Glu Leu Asp Thr Glu Ala Glu Val Ser Arg Arg Leu Glu Asn 
65                  70                  75                  80  


Arg Leu Val Leu Leu Phe Phe Gly Ala Gly Ala Cys Pro Gln Cys Gln 
                85                  90                  95      


Ala Phe Val Pro Ile Leu Lys Asp Phe Phe Val Arg Leu Thr Asp Glu 
            100                 105                 110         


Phe Tyr Val Leu Arg Ala Ala Gln Leu Ala Leu Val Tyr Val Ser Gln 
        115                 120                 125             


Asp Ser Thr Glu Glu Gln Gln Asp Leu Phe Leu Lys Asp Met Pro Lys 
    130                 135                 140                 


Lys Trp Leu Phe Leu Pro Phe Glu Asp Asp Leu Arg Arg 
145                 150                 155         


<210>  13
<211>  616
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GUSB signal peptide-furin-RdCVF(SF)

<400>  13
gaattcctcc ggggaccaca cgccgcgctg tccccagcac ttaccatggc ccgggggtcg       60

gcggttgcct gggcggcgct cgggccgttg ttgtggggct gcgcgctggg gctgcagatg      120

cccctcgagt ccggcttgct gtcctccgag gactccgcca gctccgagag cttcgccaag      180

cgcatcaagc gcatggcctc cctgttctct ggccgcatcc tgatccgcaa caatagcgac      240

caggacgagc tggatacgga ggctgaggtc agtcgcaggc tggagaaccg gctggtgctg      300

ctgttctttg gtgctggggc ttgtccacag tgccaggcct tcgtgcccat cctcaaggac      360

ttcttcgtgc ggctcacaga tgagttctat gtactgcggg cggctcagct ggccctggtg      420

tacgtgtccc aggactccac ggaggagcag caggacctgt tcctcaagga catgccaaag      480

aaatggcttt tcctgccctt tgaggatgat ctgaggaggt gaggccccag ggaagaccag      540

ggagggcttc ctggagaagg catttccctg gaggtttact gtcctggtac tacttgtgca      600

taaagaggta aagctt                                                      616


<210>  14
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BDNF signal peptide-furin-RdCVF(SF)

<400>  14

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro Met Pro Leu Glu Ser Gly Leu Ser Ser Glu Asp Ser 
            20                  25                  30          


Ala Ser Ser Glu Ser Phe Ala Lys Arg Ile Lys Arg Met Ala Ser Leu 
        35                  40                  45              


Phe Ser Gly Arg Ile Leu Ile Arg Asn Asn Ser Asp Gln Asp Glu Leu 
    50                  55                  60                  


Asp Thr Glu Ala Glu Val Ser Arg Arg Leu Glu Asn Arg Leu Val Leu 
65                  70                  75                  80  


Leu Phe Phe Gly Ala Gly Ala Cys Pro Gln Cys Gln Ala Phe Val Pro 
                85                  90                  95      


Ile Leu Lys Asp Phe Phe Val Arg Leu Thr Asp Glu Phe Tyr Val Leu 
            100                 105                 110         


Arg Ala Ala Gln Leu Ala Leu Val Tyr Val Ser Gln Asp Ser Thr Glu 
        115                 120                 125             


Glu Gln Gln Asp Leu Phe Leu Lys Asp Met Pro Lys Lys Trp Leu Phe 
    130                 135                 140                 


Leu Pro Phe Glu Asp Asp Leu Arg Arg 
145                 150             


<210>  15
<211>  601
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BDNF signal peptide-furin-RdCVF(SF)

<400>  15
gaattcctcc ggggaccaca cgccgcgctg tccccagcac ttaccatgac catccttttc       60

cttactatgg ttatttcata ctttggttgc atgaaggctg cccccatgcc cctcgagtcc      120

ggcctgtcct ccgaggactc cgccagctcc gagagcttcg ccaagcgcat caagcgcatg      180

gcctccctgt tctctggccg catcctgatc cgcaacaata gcgaccagga cgagctggat      240

acggaggctg aggtcagtcg caggctggag aaccggctgg tgctgctgtt ctttggtgct      300

ggggcttgtc cacagtgcca ggccttcgtg cccatcctca aggacttctt cgtgcggctc      360

acagatgagt tctatgtact gcgggcggct cagctggccc tggtgtacgt gtcccaggac      420

tccacggagg agcagcagga cctgttcctc aaggacatgc caaagaaatg gcttttcctg      480

ccctttgagg atgatctgag gaggtgaggc cccagggaag accagggagg gcttcctgga      540

gaaggcattt ccctggaggt ttactgtcct ggtactactt gtgcataaag aggtaaagct      600

t                                                                      601


<210>  16
<211>  183
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IGF1 signal peptide-furin-RdCVF(SF)

<400>  16

Met Gly Lys Ile Ser Ser Leu Pro Thr Gln Leu Phe Lys Cys Cys Phe 
1               5                   10                  15      


Cys Asp Phe Leu Lys Val Lys Met His Thr Met Ser Ser Ser His Leu 
            20                  25                  30          


Phe Tyr Leu Ala Leu Cys Leu Leu Thr Phe Thr Ser Ser Ala Thr Ala 
        35                  40                  45              


Gly Pro Met Pro Leu Glu Ser Gly Leu Ser Ser Glu Asp Ser Ala Ser 
    50                  55                  60                  


Ser Glu Ser Phe Ala Lys Arg Ile Lys Arg Met Ala Ser Leu Phe Ser 
65                  70                  75                  80  


Gly Arg Ile Leu Ile Arg Asn Asn Ser Asp Gln Asp Glu Leu Asp Thr 
                85                  90                  95      


Glu Ala Glu Val Ser Arg Arg Leu Glu Asn Arg Leu Val Leu Leu Phe 
            100                 105                 110         


Phe Gly Ala Gly Ala Cys Pro Gln Cys Gln Ala Phe Val Pro Ile Leu 
        115                 120                 125             


Lys Asp Phe Phe Val Arg Leu Thr Asp Glu Phe Tyr Val Leu Arg Ala 
    130                 135                 140                 


Ala Gln Leu Ala Leu Val Tyr Val Ser Gln Asp Ser Thr Glu Glu Gln 
145                 150                 155                 160 


Gln Asp Leu Phe Leu Lys Asp Met Pro Lys Lys Trp Leu Phe Leu Pro 
                165                 170                 175     


Phe Glu Asp Asp Leu Arg Arg 
            180             


<210>  17
<211>  694
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IGF1 signal peptide-furin-RdCVF(SF)

<400>  17
gaattcctcc ggggaccaca cgccgcgctg tccccagcac ttaccatggg aaaaatcagc       60

agtcttccaa cccaattatt taagtgctgc ttttgtgatt tcttgaaggt gaagatgcac      120

accatgtcct cctcgcatct cttctacctg gcgctgtgcc tgctcacctt caccagctct      180

gccacggctg gaccggagat gcccctcgag tccggcctgt cctccgagga ctccgccagc      240

tccgagagct tcgccaagcg catcaagcgc atggcctccc tgttctctgg ccgcatcctg      300

atccgcaaca atagcgacca ggacgagctg gatacggagg ctgaggtcag tcgcaggctg      360

gagaaccggc tggtgctgct gttctttggt gctggggctt gtccacagtg ccaggccttc      420

gtgcccatcc tcaaggactt cttcgtgcgg ctcacagatg agttctatgt actgcgggcg      480

gctcagctgg ccctggtgta cgtgtcccag gactccacgg aggagcagca ggacctgttc      540

ctcaaggaca tgccaaagaa atggcttttc ctgccctttg aggatgatct gaggaggtga      600

ggccccaggg aagaccaggg agggcttcct ggagaaggca tttccctgga ggtttactgt      660

cctggtacta cttgtgcata aagaggtaaa gctt                                  694


<210>  18
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human growth hormone signal peptide-RdCVF(LF)

<400>  18
atggctacag gctcccggac gtccctgctc ctggcttttg gcctgctctg cctcccctgg       60

cttcaagagg gatccgccgc ctccctgttc tctggccgca tcctgatccg caacaatagc      120

gaccaggacg agctggatac ggaggctgag gtcagtcgca ggctggagaa ccggctggtg      180

ctgctgttct ttggtgctgg ggcttgtcca cagtgccagg ccttcgtgcc catcctcaag      240

gacttcttcg tgcggctcac agatgagttc tatgtactgc gggcggctca gctggccctg      300

gtgtacgtgt cccaggactc cacggaggag cagcaggacc tgttcctcaa ggacatgcca      360

aagaaatggc ttttcctgcc ctttgaggat gatctgagga gggacctcgg gcgccagttc      420

tcagtggagc gcctgccggc ggtcgtggtg ctcaagccgg acggggacgt gctcactcgc      480

gacggcgccg acgagatcca gcgcctgggc accgcctgct tcgccaactg gcaggaggcg      540

gccgaggtgc tggaccgcaa cttccagctg ccagaggacc tggaggacca ggagccacgg      600

agcctcaccg agtgcctgcg ccgccacaag taccgcgtgg aaaaggcggc gcgaggcggg      660

cgcgaccccg ggggaggggg tggggaggag ggcggggccg gggggctgtt ctga            714


<210>  19
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human growth hormone signal peptide-RdCVF(LF)

<400>  19

Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly Leu Leu 
1               5                   10                  15      


Cys Leu Pro Trp Leu Gln Glu Gly Ser Ala Ala Ser Leu Phe Ser Gly 
            20                  25                  30          


Arg Ile Leu Ile Arg Asn Asn Ser Asp Gln Asp Glu Leu Asp Thr Glu 
        35                  40                  45              


Ala Glu Val Ser Arg Arg Leu Glu Asn Arg Leu Val Leu Leu Phe Phe 
    50                  55                  60                  


Gly Ala Gly Ala Cys Pro Gln Cys Gln Ala Phe Val Pro Ile Leu Lys 
65                  70                  75                  80  


Asp Phe Phe Val Arg Leu Thr Asp Glu Tyr Val Leu Arg Ala Ala Gln 
                85                  90                  95      


Leu Ala Leu Val Tyr Val Ser Gln Asp Ser Thr Glu Glu Gln Gln Asp 
            100                 105                 110         


Leu Phe Leu Lys Asp Met Pro Lys Lys Trp Leu Phe Leu Pro Phe Glu 
        115                 120                 125             


Asp Asp Leu Arg Arg Asp Leu Gly Arg Gln Phe Ser Val Glu Arg Leu 
    130                 135                 140                 


Pro Ala Val Val Val Leu Lys Pro Asp Gly Asp Val Leu Thr Arg Asp 
145                 150                 155                 160 


Gly Ala Asp Glu Ile Gln Arg Leu Gly Thr Ala Cys Phe Ala Asn Trp 
                165                 170                 175     


Gln Glu Ala Ala Glu Val Leu Asp Arg Asn Phe Gln Leu Pro Glu Asp 
            180                 185                 190         


Leu Glu Asp Gln Glu Pro Arg Ser Leu Thr Glu Cys Leu Arg Arg His 
        195                 200                 205             


Lys Tyr Arg Val Glu Lys Ala Ala Arg Gly Gly Arg Asp Pro Gly Gly 
    210                 215                 220                 


Gly Gly Gly Glu Glu Gly Gly Ala Gly Gly Leu Phe 
225                 230                 235     


<210>  20
<211>  405
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  growth hormone signal peptide - RdCVF(SF)

<400>  20
atggctacag gctcccggac gtccctgctc ctggcttttg gcctgctctg cctcccctgg       60

cttcaagagg gatccgccgc ctccctgttc tctggccgca tcctgatccg caacaatagc      120

gaccaggacg agctggatac ggaggctgag gtcagtcgca ggctggagaa ccggctggtg      180

ctgctgttct ttggtgctgg ggcttgtcca cagtgccagg ccttcgtgcc catcctcaag      240

gacttcttcg tgcggctcac agatgagttc tatgtactgc gggcggctca gctggccctg      300

gtgtacgtgt cccaggactc cacggaggag cagcaggacc tgttcctcaa ggacatgcca      360

aagaaatggc ttttcctgcc ctttgaggat gatctgagga ggtga                      405


<210>  21
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hGH signal peptide - RdCVF(SF)

<400>  21

Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly Leu Leu 
1               5                   10                  15      


Cys Leu Pro Trp Leu Gln Glu Gly Ser Ala Ala Ser Leu Phe Ser Gly 
            20                  25                  30          


Arg Ile Leu Ile Arg Asn Asn Ser Asp Gln Asp Glu Leu Asp Thr Glu 
        35                  40                  45              


Ala Glu Val Ser Arg Arg Leu Glu Asn Arg Leu Val Leu Leu Phe Phe 
    50                  55                  60                  


Gly Ala Gly Ala Cys Pro Gln Cys Gln Ala Phe Val Pro Ile Leu Lys 
65                  70                  75                  80  


Asp Phe Phe Val Arg Leu Thr Asp Glu Phe Tyr Leu Arg Ala Ala Gln 
                85                  90                  95      


Leu Ala Leu Val Tyr Val Ser Gln Asp Ser Thr Glu Glu Gln Gln Asp 
            100                 105                 110         


Leu Phe Leu Lys Asp Met Pro Lys Lys Trp Leu Phe Leu Pro Phe Glu 
        115                 120                 125             


Asp Asp Leu Arg Arg 
    130             


<210>  22
<211>  750
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hGH signal peptide - RdCVF(LF)-HPC4

<400>  22
atggctacag gctcccggac gtccctgctc ctggcttttg gcctgctctg cctcccctgg       60

cttcaagagg gatccgccgc ctccctgttc tctggccgca tcctgatccg caacaatagc      120

gaccaggacg agctggatac ggaggctgag gtcagtcgca ggctggagaa ccggctggtg      180

ctgctgttct ttggtgctgg ggcttgtcca cagtgccagg ccttcgtgcc catcctcaag      240

gacttcttcg tgcggctcac agatgagttc tatgtactgc gggcggctca gctggccctg      300

gtgtacgtgt cccaggactc cacggaggag cagcaggacc tgttcctcaa ggacatgcca      360

aagaaatggc ttttcctgcc ctttgaggat gatctgagga gggacctcgg gcgccagttc      420

tcagtggagc gcctgccggc ggtcgtggtg ctcaagccgg acggggacgt gctcactcgc      480

gacggcgccg acgagatcca gcgcctgggc accgcctgct tcgccaactg gcaggaggcg      540

gccgaggtgc tggaccgcaa cttccagctg ccagaggacc tggaggacca ggagccacgg      600

agcctcaccg agtgcctgcg ccgccacaag taccgcgtgg aaaaggcggc gcgaggcggg      660

cgcgaccccg ggggaggggg tggggaggag ggcggggccg gggggctgtt cgaagatcaa      720

gtcgatccac gcctcatcga tggcaaatga                                       750


<210>  23
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hGH signal peptide - RdCVF(LF)-HPC4

<400>  23

Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly Leu Leu 
1               5                   10                  15      


Cys Leu Pro Trp Leu Gln Glu Gly Ser Ala Ala Ser Leu Phe Ser Gly 
            20                  25                  30          


Arg Ile Leu Ile Arg Asn Asn Ser Asp Gln Asp Glu Leu Asp Thr Glu 
        35                  40                  45              


Ala Glu Val Ser Arg Arg Leu Glu Asn Arg Leu Val Leu Leu Phe Phe 
    50                  55                  60                  


Gly Ala Gly Ala Cys Pro Gln Cys Gln Ala Phe Val Pro Ile Leu Lys 
65                  70                  75                  80  


Asp Phe Phe Val Arg Leu Thr Asp Glu Tyr Val Leu Arg Ala Ala Gln 
                85                  90                  95      


Leu Ala Leu Val Tyr Val Ser Gln Asp Ser Thr Glu Glu Gln Gln Asp 
            100                 105                 110         


Leu Phe Leu Lys Asp Met Pro Lys Lys Trp Leu Phe Leu Pro Phe Glu 
        115                 120                 125             


Asp Asp Leu Arg Arg Asp Leu Gly Arg Gln Phe Ser Val Glu Arg Leu 
    130                 135                 140                 


Pro Ala Val Val Val Leu Lys Pro Asp Gly Asp Val Leu Thr Arg Asp 
145                 150                 155                 160 


Gly Ala Asp Glu Ile Gln Arg Leu Gly Thr Ala Cys Phe Ala Asn Trp 
                165                 170                 175     


Gln Glu Ala Ala Glu Val Leu Asp Arg Asn Phe Gln Leu Pro Glu Asp 
            180                 185                 190         


Leu Glu Asp Gln Glu Pro Arg Ser Leu Thr Glu Cys Leu Arg Arg His 
        195                 200                 205             


Lys Tyr Arg Val Glu Lys Ala Ala Arg Gly Gly Arg Asp Pro Gly Gly 
    210                 215                 220                 


Gly Gly Gly Glu Glu Gly Gly Ala Gly Gly Leu Phe Glu Asp Gln Val 
225                 230                 235                 240 


Asp Pro Arg Leu Ile Asp Gly Lys 
                245             


<210>  24
<211>  441
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hGH signal peptide - RdCVF(SF)-HPC4

<400>  24
atggctacag gctcccggac gtccctgctc ctggcttttg gcctgctctg cctcccctgg       60

cttcaagagg gatccgccgc ctccctgttc tctggccgca tcctgatccg caacaatagc      120

gaccaggacg agctggatac ggaggctgag gtcagtcgca ggctggagaa ccggctggtg      180

ctgctgttct ttggtgctgg ggcttgtcca cagtgccagg ccttcgtgcc catcctcaag      240

gacttcttcg tgcggctcac agatgagttc tatgtactgc gggcggctca gctggccctg      300

gtgtacgtgt cccaggactc cacggaggag cagcaggacc tgttcctcaa ggacatgcca      360

aagaaatggc ttttcctgcc ctttgaggat gatctgagga gggaagatca agtcgatcca      420

cgcctcatcg atggcaaatg a                                                441


<210>  25
<211>  145
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hGH signal peptide - RdCVF(SF)-HPC4

<400>  25

Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly Leu Leu 
1               5                   10                  15      


Cys Leu Pro Trp Leu Gln Glu Gly Ser Ala Ala Ser Leu Phe Ser Gly 
            20                  25                  30          


Arg Ile Leu Ile Arg Asn Asn Ser Asp Gln Asp Glu Leu Asp Thr Glu 
        35                  40                  45              


Ala Glu Val Ser Arg Arg Leu Glu Asn Arg Leu Val Leu Leu Phe Phe 
    50                  55                  60                  


Gly Ala Gly Ala Cys Pro Gln Cys Gln Ala Phe Val Pro Ile Leu Lys 
65                  70                  75                  80  


Asp Phe Phe Val Arg Leu Thr Asp Glu Phe Tyr Leu Arg Ala Ala Gln 
                85                  90                  95      


Leu Ala Leu Val Tyr Val Ser Gln Asp Ser Thr Glu Glu Gln Gln Asp 
            100                 105                 110         


Leu Phe Leu Lys Asp Met Pro Lys Lys Trp Leu Phe Leu Pro Phe Glu 
        115                 120                 125             


Asp Asp Leu Arg Arg Glu Asp Gln Val Asp Pro Arg Leu Ile Asp Gly 
    130                 135                 140                 


Lys 
145 


<210>  26
<211>  212
<212>  PRT
<213>  Homo sapiens

<400>  26

Met Ala Ser Leu Phe Ser Gly Arg Ile Leu Ile Arg Asn Asn Ser Asp 
1               5                   10                  15      


Gln Asp Glu Leu Asp Thr Glu Ala Glu Val Ser Arg Arg Leu Glu Asn 
            20                  25                  30          


Arg Leu Val Leu Leu Phe Phe Gly Ala Gly Ala Cys Pro Gln Cys Gln 
        35                  40                  45              


Ala Phe Val Pro Ile Leu Lys Asp Phe Phe Val Arg Leu Thr Asp Glu 
    50                  55                  60                  


Phe Tyr Val Leu Arg Ala Ala Gln Leu Ala Leu Val Tyr Val Ser Gln 
65                  70                  75                  80  


Asp Ser Thr Glu Glu Gln Gln Asp Leu Phe Leu Lys Asp Met Pro Lys 
                85                  90                  95      


Lys Trp Leu Phe Leu Pro Phe Glu Asp Asp Leu Arg Arg Asp Leu Gly 
            100                 105                 110         


Arg Gln Phe Ser Val Glu Arg Leu Pro Ala Val Val Val Leu Lys Pro 
        115                 120                 125             


Asp Gly Asp Val Leu Thr Arg Asp Gly Ala Asp Glu Ile Gln Arg Leu 
    130                 135                 140                 


Gly Thr Ala Cys Phe Ala Asn Trp Gln Glu Ala Ala Glu Val Leu Asp 
145                 150                 155                 160 


Arg Asn Phe Gln Leu Pro Glu Asp Leu Glu Asp Gln Glu Pro Arg Ser 
                165                 170                 175     


Leu Thr Glu Cys Leu Arg Arg His Lys Tyr Arg Val Glu Lys Ala Ala 
            180                 185                 190         


Arg Gly Gly Arg Asp Pro Gly Gly Gly Gly Gly Glu Glu Gly Gly Ala 
        195                 200                 205             


Gly Gly Leu Phe 
    210         


<210>  27
<211>  109
<212>  PRT
<213>  Homo sapiens

<400>  27

Met Ala Ser Leu Phe Ser Gly Arg Ile Leu Ile Arg Asn Asn Ser Asp 
1               5                   10                  15      


Gln Asp Glu Leu Asp Thr Glu Ala Glu Val Ser Arg Arg Leu Glu Asn 
            20                  25                  30          


Arg Leu Val Leu Leu Phe Phe Gly Ala Gly Ala Cys Pro Gln Cys Gln 
        35                  40                  45              


Ala Phe Val Pro Ile Leu Lys Asp Phe Phe Val Arg Leu Thr Asp Glu 
    50                  55                  60                  


Phe Tyr Val Leu Arg Ala Ala Gln Leu Ala Leu Val Tyr Val Ser Gln 
65                  70                  75                  80  


Asp Ser Thr Glu Glu Gln Gln Asp Leu Phe Leu Lys Asp Met Pro Lys 
                85                  90                  95      


Lys Trp Leu Phe Leu Pro Phe Glu Asp Asp Leu Arg Arg 
            100                 105                 


<210>  28
<211>  26
<212>  PRT
<213>  Homo sapiens

<400>  28

Met Ala Thr Gly Ser Arg Thr Ser Leu Leu Leu Ala Phe Gly Leu Leu 
1               5                   10                  15      


Cys Leu Pro Trp Leu Gln Glu Gly Ser Ala 
            20                  25      


<210>  29
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  29

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala Ala Pro 
            20  


<210>  30
<211>  50
<212>  PRT
<213>  Homo sapiens

<400>  30

Met Gly Lys Ile Ser Ser Leu Pro Thr Gln Leu Phe Lys Cys Cys Phe 
1               5                   10                  15      


Cys Asp Phe Leu Lys Val Lys Met His Thr Met Ser Ser Ser His Leu 
            20                  25                  30          


Phe Tyr Leu Ala Leu Cys Leu Leu Thr Phe Thr Ser Ser Ala Thr Ala 
        35                  40                  45              


Gly Pro 
    50  


<210>  31
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  31

Met Ala Arg Gly Ser Ala Val Ala Trp Ala Ala Leu Gly Pro Leu Leu 
1               5                   10                  15      


Trp Gly Cys Ala Leu Gly Leu Gln 
            20                  


<210>  32
<211>  212
<212>  PRT
<213>  Homo sapiens

<400>  32

Met Ala Ser Leu Phe Ser Gly Arg Ile Leu Ile Arg Asn Asn Ser Asp 
1               5                   10                  15      


Gln Asp Glu Leu Asp Thr Glu Ala Glu Val Ser Arg Arg Leu Glu Asn 
            20                  25                  30          


Arg Leu Val Leu Leu Phe Phe Gly Ala Gly Ala Cys Pro Gln Cys Gln 
        35                  40                  45              


Ala Phe Val Pro Ile Leu Lys Asp Phe Phe Val Arg Leu Thr Asp Glu 
    50                  55                  60                  


Phe Tyr Val Leu Arg Ala Ala Gln Leu Ala Leu Val Tyr Val Ser Gln 
65                  70                  75                  80  


Asp Ser Thr Glu Glu Gln Gln Asp Leu Phe Leu Lys Asp Met Pro Lys 
                85                  90                  95      


Lys Trp Leu Phe Leu Pro Phe Glu Asp Asp Leu Arg Arg Asp Leu Gly 
            100                 105                 110         


Arg Gln Phe Ser Val Glu Arg Leu Pro Ala Val Val Val Leu Lys Pro 
        115                 120                 125             


Asp Gly Asp Val Leu Thr Arg Asp Gly Ala Asp Glu Ile Gln Arg Leu 
    130                 135                 140                 


Gly Thr Ala Cys Phe Ala Asn Trp Gln Glu Ala Ala Glu Val Leu Asp 
145                 150                 155                 160 


Arg Asn Phe Gln Leu Pro Glu Asp Leu Glu Asp Gln Glu Pro Arg Ser 
                165                 170                 175     


Leu Thr Glu Cys Leu Arg Arg His Lys Tyr Arg Val Glu Lys Ala Ala 
            180                 185                 190         


Arg Gly Gly Arg Asp Pro Gly Gly Gly Gly Gly Glu Glu Gly Gly Ala 
        195                 200                 205             


Gly Gly Leu Phe 
    210         


<210>  33
<211>  948
<212>  DNA
<213>  Homo sapiens

<400>  33
ccggggacca cacgccgcgc tgtccccagc acccaaccca ggttaccatg gcctccctgt       60

tctctggccg catcctgatc cgcaacaata gcgaccagga cgagctggat acggaggctg      120

aggtcagtcg caggctggag aaccggctgg tgctgctgtt ctttggtgct ggggcttgtc      180

cacagtgcca ggccttcgtg cccatcctca aggacttctt cgtgcggctc acagatgagt      240

tctatgtact gcgggcggct cagctggccc tggtgtacgt gtcccaggac tccacggagg      300

agcagcagga cctgttcctc aaggacatgc caaagaaatg gcttttcctg ccctttgagg      360

atgatctgag gagggacctc gggcgccagt tctcagtgga gcgcctgccg gcggtcgtgg      420

tgctcaagcc ggacggggac gtgctcactc gcgacggcgc cgacgagatc cagcgcctgg      480

gcaccgcctg cttcgccaac tggcaggagg cggccgaggt gctggaccgc aacttccagc      540

tgccagagga cctggaggac caggagccac ggagcctcac cgagtgcctg cgccgccaca      600

agtaccgcgt ggaaaaggcg gcgcgaggcg ggcgcgaccc cgggggaggg ggtggggagg      660

agggcggggc cggggggctg ttctgacccg ctagggtgga ggagaggagt ggggtttgtt      720

gatgaacctc cacccccacc ccacccccgc acgcctgtaa tcccagcact tggggaggcc      780

aaggcgggag gatcgcttga gcccagaggt tcgagatcaa cctgggcaag agagtgagac      840

cctgactcta cgaaaattaa aagttagccc ggtgtggtgg cgcgcacctg tggcttagct      900

accctgaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa                   948


