                         SEQUENCE LISTING

<110>  Lavie, Arnon D.
       Konrad, Manfred
 
<120>  USE OF SPECIFICALLY ENGINEERED ENZYMES TO ENHANCE THE EFFICACY OF
        PRODRUGS

<130>  02-134-H-WO

<150>  61/054,400
<151>  2008-05-19

<150>  61/054,403
<151>  2008-05-19

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  260
<212>  PRT
<213>  Homo sapiens - dCK

<400>  1

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Ser Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Ala Cys Leu Ser Arg Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Asp Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  2
<211>  277
<212>  PRT
<213>  Homo sapiens - dGK

<400>  2

Met Ala Ala Gly Arg Leu Phe Leu Ser Arg Leu Arg Ala Pro Phe Ser 
1               5                   10                  15      


Ser Met Ala Lys Ser Pro Leu Glu Gly Val Ser Ser Ser Arg Gly Leu 
            20                  25                  30          


His Ala Gly Arg Gly Pro Arg Arg Leu Ser Ile Glu Gly Asn Ile Ala 
        35                  40                  45              


Val Gly Lys Ser Thr Phe Val Lys Leu Leu Thr Lys Thr Tyr Pro Glu 
    50                  55                  60                  


Trp His Val Ala Thr Glu Pro Val Ala Thr Trp Gln Asn Ile Gln Ala 
65                  70                  75                  80  


Ala Gly Asn Gln Lys Ala Cys Thr Ala Gln Ser Leu Gly Asn Leu Leu 
                85                  90                  95      


Asp Met Met Tyr Arg Glu Pro Ala Arg Trp Ser Tyr Thr Phe Gln Thr 
            100                 105                 110         


Phe Ser Phe Leu Ser Arg Leu Lys Val Gln Leu Glu Pro Phe Pro Glu 
        115                 120                 125             


Lys Leu Leu Gln Ala Arg Lys Pro Val Gln Ile Phe Glu Arg Ser Val 
    130                 135                 140                 


Tyr Ser Asp Arg Tyr Ile Phe Ala Lys Asn Leu Phe Glu Asn Gly Ser 
145                 150                 155                 160 


Leu Ser Asp Ile Glu Trp His Ile Tyr Gln Asp Trp His Ser Phe Leu 
                165                 170                 175     


Leu Trp Glu Phe Ala Ser Arg Ile Thr Leu His Gly Phe Ile Tyr Leu 
            180                 185                 190         


Gln Ala Ser Pro Gln Val Cys Leu Lys Arg Leu Tyr Gln Arg Ala Arg 
        195                 200                 205             


Glu Glu Glu Lys Gly Ile Glu Leu Ala Tyr Leu Glu Gln Leu His Gly 
    210                 215                 220                 


Gln His Glu Ala Trp Leu Ile His Lys Thr Thr Lys Leu His Phe Glu 
225                 230                 235                 240 


Ala Leu Met Asn Ile Pro Val Leu Val Leu Asp Val Asn Asp Asp Phe 
                245                 250                 255     


Ser Glu Glu Val Thr Lys Gln Glu Asp Leu Met Arg Glu Val Asn Thr 
            260                 265                 270         


Phe Val Lys Asn Leu 
        275         


<210>  3
<211>  250
<212>  PRT
<213>  Drosophila melanogaster - dNK

<400>  3

Met Ala Glu Ala Ala Ser Cys Ala Arg Lys Gly Thr Lys Tyr Ala Glu 
1               5                   10                  15      


Gly Thr Gln Pro Phe Thr Val Leu Ile Glu Gly Asn Ile Gly Ser Gly 
            20                  25                  30          


Lys Thr Thr Tyr Leu Asn His Phe Glu Lys Tyr Lys Asn Asp Ile Cys 
        35                  40                  45              


Leu Leu Thr Glu Pro Val Glu Lys Trp Arg Asn Val Asn Gly Val Asn 
    50                  55                  60                  


Leu Leu Glu Leu Met Tyr Lys Asp Pro Lys Lys Trp Ala Met Pro Phe 
65                  70                  75                  80  


Gln Ser Tyr Val Thr Leu Thr Met Leu Gln Ser His Thr Ala Pro Thr 
                85                  90                  95      


Asn Lys Lys Leu Lys Ile Met Glu Arg Ser Ile Phe Ser Ala Arg Tyr 
            100                 105                 110         


Cys Phe Val Glu Asn Met Arg Arg Asn Gly Ser Leu Glu Gln Gly Met 
        115                 120                 125             


Tyr Asn Thr Leu Glu Glu Trp Tyr Lys Phe Ile Glu Glu Ser Ile His 
    130                 135                 140                 


Val Gln Ala Asp Leu Ile Ile Tyr Leu Arg Thr Ser Pro Glu Val Ala 
145                 150                 155                 160 


Tyr Glu Arg Ile Arg Gln Arg Ala Arg Ser Glu Glu Ser Cys Val Pro 
                165                 170                 175     


Leu Lys Tyr Leu Gln Glu Leu His Glu Leu His Glu Asp Trp Leu Ile 
            180                 185                 190         


His Gln Arg Arg Pro Gln Ser Cys Lys Val Leu Val Leu Asp Ala Asp 
        195                 200                 205             


Leu Asn Leu Glu Asn Ile Gly Thr Glu Tyr Gln Arg Ser Glu Ser Ser 
    210                 215                 220                 


Ile Phe Asp Ala Ile Ser Ser Asn Gln Gln Pro Ser Pro Val Leu Val 
225                 230                 235                 240 


Ser Pro Ser Lys Arg Gln Arg Val Ala Arg 
                245                 250 


<210>  4
<211>  232
<212>  PRT
<213>  Homo sapiens - mitochondrial TK2

<400>  4

Val Gln Arg Tyr Ala Trp Pro Pro Asp Lys Glu Gln Glu Lys Glu Lys 
1               5                   10                  15      


Lys Ser Val Ile Cys Val Glu Gly Asn Ile Ala Ser Gly Lys Thr Thr 
            20                  25                  30          


Cys Leu Glu Phe Phe Ser Asn Ala Thr Asp Val Glu Val Leu Thr Glu 
        35                  40                  45              


Pro Val Ser Lys Trp Arg Asn Val Arg Gly His Asn Pro Leu Gly Leu 
    50                  55                  60                  


Met Tyr His Asp Ala Ser Arg Trp Gly Leu Thr Leu Gln Thr Tyr Val 
65                  70                  75                  80  


Gln Leu Thr Met Leu Asp Arg His Thr Arg Pro Gln Val Ser Ser Val 
                85                  90                  95      


Arg Leu Met Glu Arg Ser Ile His Ser Ala Arg Tyr Ile Phe Val Glu 
            100                 105                 110         


Asn Leu Tyr Arg Ser Gly Lys Met Pro Glu Val Asp Tyr Val Val Leu 
        115                 120                 125             


Ser Glu Trp Phe Asp Trp Ile Leu Arg Asn Met Asp Val Ser Val Asp 
    130                 135                 140                 


Leu Ile Val Tyr Leu Arg Thr Asn Pro Glu Thr Cys Tyr Gln Arg Leu 
145                 150                 155                 160 


Lys Lys Arg Cys Arg Glu Glu Glu Lys Val Ile Pro Leu Glu Tyr Leu 
                165                 170                 175     


Glu Ala Ile His His Leu His Glu Glu Trp Leu Ile Lys Gly Ser Leu 
            180                 185                 190         


Phe Pro Met Ala Ala Pro Val Leu Val Ile Glu Ala Asp His His Met 
        195                 200                 205             


Glu Arg Met Leu Glu Leu Phe Glu Gln Asn Arg Asp Arg Ile Leu Thr 
    210                 215                 220                 


Pro Glu Asn Arg Lys His Cys Pro 
225                 230         


<210>  5
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - A100V/R104M/D133A human dCK mutant

<400>  5

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Ser Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Val Cys Leu Ser Met Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  6
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - R104M/D133A human dCK mutant

<400>  6

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Ser Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Ala Cys Leu Ser Met Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  7
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - A100V/R104Q/D133A human dCK mutant

<400>  7

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Ser Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Val Cys Leu Ser Gln Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  8
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - R104Q/D133A human dCK mutant

<400>  8

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Ser Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Ala Cys Leu Ser Gln Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  9
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - A100V/R104L/D133A human dCK mutant

<400>  9

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Ser Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Val Cys Leu Ser Leu Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  10
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - R104L/D133A human dCK mutant

<400>  10

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Ser Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Ala Cys Leu Ser Leu Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  11
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - A100V/R104I/D133A human dCK mutant

<400>  11

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Ser Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Val Cys Leu Ser Ile Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  12
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - R104I/D133A human dCK mutant

<400>  12

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Ser Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Ala Cys Leu Ser Ile Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  13
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - S74E/A100V/R104Q/D133A human dCK mutant

<400>  13

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Glu Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Val Cys Leu Ser Gln Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  14
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - S74E/R104Q/D133A human dCK mutant

<400>  14

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Glu Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Ala Cys Leu Ser Gln Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  15
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - S74E/A100V/R104L/D133A human dCK mutant

<400>  15

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Glu Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Val Cys Leu Ser Leu Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  16
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - S74E/R104L/D133A human dCK mutant

<400>  16

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Glu Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Ala Cys Leu Ser Leu Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  17
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - S74E/A100V/R104I/D133A human dCK mutant

<400>  17

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Glu Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Val Cys Leu Ser Ile Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  18
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - S74E/R104I/D133A human dCK mutant

<400>  18

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Glu Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Ala Cys Leu Ser Ile Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  19
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - S74E/A100V/R104M/D133A human dCK mutant

<400>  19

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Glu Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Val Cys Leu Ser Met Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  20
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - S74E/R104M/D133A human dCK mutant

<400>  20

Met Ala Thr Pro Pro Lys Arg Ser Cys Pro Ser Phe Ser Ala Ser Ser 
1               5                   10                  15      


Glu Gly Thr Arg Ile Lys Lys Ile Ser Ile Glu Gly Asn Ile Ala Ala 
            20                  25                  30          


Gly Lys Ser Thr Phe Val Asn Ile Leu Lys Gln Leu Cys Glu Asp Trp 
        35                  40                  45              


Glu Val Val Pro Glu Pro Val Ala Arg Trp Cys Asn Val Gln Ser Thr 
    50                  55                  60                  


Gln Asp Glu Phe Glu Glu Leu Thr Met Glu Gln Lys Asn Gly Gly Asn 
65                  70                  75                  80  


Val Leu Gln Met Met Tyr Glu Lys Pro Glu Arg Trp Ser Phe Thr Phe 
                85                  90                  95      


Gln Thr Tyr Ala Cys Leu Ser Met Ile Arg Ala Gln Leu Ala Ser Leu 
            100                 105                 110         


Asn Gly Lys Leu Lys Asp Ala Glu Lys Pro Val Leu Phe Phe Glu Arg 
        115                 120                 125             


Ser Val Tyr Ser Ala Arg Tyr Ile Phe Ala Ser Asn Leu Tyr Glu Ser 
    130                 135                 140                 


Glu Cys Met Asn Glu Thr Glu Trp Thr Ile Tyr Gln Asp Trp His Asp 
145                 150                 155                 160 


Trp Met Asn Asn Gln Phe Gly Gln Ser Leu Glu Leu Asp Gly Ile Ile 
                165                 170                 175     


Tyr Leu Gln Ala Thr Pro Glu Thr Cys Leu His Arg Ile Tyr Leu Arg 
            180                 185                 190         


Gly Arg Asn Glu Glu Gln Gly Ile Pro Leu Glu Tyr Leu Glu Lys Leu 
        195                 200                 205             


His Tyr Lys His Glu Ser Trp Leu Leu His Arg Thr Leu Lys Thr Asn 
    210                 215                 220                 


Phe Asp Tyr Leu Gln Glu Val Pro Ile Leu Thr Leu Asp Val Asn Glu 
225                 230                 235                 240 


Asp Phe Lys Asp Lys Tyr Glu Ser Leu Val Glu Lys Val Lys Glu Phe 
                245                 250                 255     


Leu Ser Thr Leu 
            260 


<210>  21
<211>  2618
<212>  DNA
<213>  Homo sapiens - deoxycytidine kinase (dCK)

<400>  21
gcggagcgcg cacgcgggaa cccgcgctgg aggcgggcga gggccgaggg gcagctaggg       60

agcgcggctt gaggagggcg gggccgcccc gcaggcccgc cagtgtcctc agctgcctcc      120

gcgcgccaaa gtcaaacccc gacacccgcc ggcgggccgg tgagctcact agctgacccg      180

gcaggtcagg atctggctta gcggcgccgc gagctccagt gcgcgcaccc gtggccgcct      240

cccagccctc tttgccggac gagctctggg ccgccacaag actaaggaat ggccaccccg      300

cccaagagaa gctgcccgtc tttctcagcc agctctgagg ggacccgcat caagaaaatc      360

tccatcgaag ggaacatcgc tgcagggaag tcaacatttg tgaatatcct taaacaattg      420

tgtgaagatt gggaagtggt tcctgaacct gttgccagat ggtgcaatgt tcaaagtact      480

caagatgaat ttgaggaact tacaatgtct cagaaaaatg gtgggaatgt tcttcagatg      540

atgtatgaga aacctgaacg atggtctttt accttccaaa catatgcctg tctcagtcga      600

ataagagctc agcttgcctc tctgaatggc aagctcaaag atgcagagaa acctgtatta      660

ttttttgaac gatctgtgta tagtgacagg tatatttttg catctaattt gtatgaatct      720

gaatgcatga atgagacaga gtggacaatt tatcaagact ggcatgactg gatgaataac      780

caatttggcc aaagccttga attggatgga atcatttatc ttcaagccac tccagagaca      840

tgcttacata gaatatattt acggggaaga aatgaagagc aaggcattcc tcttgaatat      900

ttagagaagc ttcattataa acatgaaagc tggctcctgc ataggacact gaaaaccaac      960

ttcgattatc ttcaagaggt gcctatctta acactggatg ttaatgaaga ctttaaagac     1020

aaatatgaaa gtctggttga aaaggtcaaa gagtttttga gtactttgtg atcttgctga     1080

agactacagg cagccaaatg gttccagata cttcagcttt gtgtatcttc gtaacttcat     1140

attaatataa gtttctttag aaaacccaag tttttaatcg tttttgtttt aaggaaaaaa     1200

gatttttaaa atgaatctta tgcaaaactt tttgaccagt ttcttttctt ttgttttttt     1260

tttaaaaaag acatttaaag acaaagacat tatttctcat agcaggaaat gtagaggtag     1320

atggttccag tatcagcata gtgactaaac tacattataa aagatccagc ttccttctgt     1380

cattcccctc ttttgtcttc ctcagcaggt tggctttttt ccctggtgcc tctcacttcg     1440

ttggtgacca gtttcttaaa ctgaaagctt taatgttaca tagtaaatgg tagtgtgtcc     1500

tgtgtaaatt agtgtaccta ttaaaagttg caaagtggaa ttaaaggaat ccctagaata     1560

aggattctga agttttattt taaattatta tcttcttaac agtttagtcc cacctcttac     1620

ttcctgcctc agtctgcttt ctctactgtc tggattaatt aggcagcctg ctataaagtt     1680

aaagtcacac atttctattt tgcaaacact gtgattactc tttgctttgt agtttgcttt     1740

gctttgtagg gttctgcttt taagtttttc tctttttcag acaaattact gataaaaatg     1800

atattgctct atatgtaata tatcctgaaa gcattatttt ttgttgaata ggaaataaaa     1860

ttaatgaaga cagaggctag aaagcatcca ttaattaatg agacacactt aactacttat     1920

ctctaaacca tctatgtgaa tatttgtaaa aataatgaat ggactcatct tagttctgta     1980

tataaatata ttttctttct agtttgttta gttaaggtgt gcagtgtttt tcctgtgtat     2040

taaacctttc cattttacgt tttagaaaat tttatgtatt ttaaaataag gggaagagtc     2100

attttcactt ttaaactact atttttcttt ccaagtcatt tttgtttttg gtttcttatt     2160

caaagatgat aatttagtgg attaaccagt ccagacgcac tgatctttgc aaaggagact     2220

taatttcaaa tctgtaatta ccatacataa actgtctcat tatacgtatg cattttttta     2280

gtttgttttt gtttggtata aattaatttg ttaattaaat atttcttaag tataaacctt     2340

atgaactaca gtggagctac actcattgaa atgtaatttc agttctaaaa agatgtaata     2400

atcattttag aattaaaatt tattctactt ttaaataaat tatgaatatt aaaggtgaaa     2460

attgtataaa ttactttgat tccattttaa gtggagacat atttcagtga tttttagtaa     2520

cctttaaaaa tgtataatga cttttaaaat ttgtagaatt gaaaagacgc taataaaaat     2580

ttattattta tttgtcatga ctcaaaaaaa aaaaaaaa                             2618


<210>  22
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Human dCK forward PCR primer

<400>  22
ggaattccat atggccaccc cgcccaagag aag                                    33


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Human dCK reverse PCR primer

<400>  23
cgcggatcct cacaaagtac tcaaaaactc                                        30


