                         SEQUENCE LISTING

<110>  Archemix Corp.
 
<120>  Aptamers that Bind to P-Selectin and Their Use as Coagulation, 
       Thrombotic, Inflammatory, and  Metastatic Disease Therapeutics

<130>  23239-601001WO

<150>  61/127629
<151>  2008-05-13

<150>  61/132182
<151>  2008-06-16

<150>  61/122642
<151>  2008-12-15

<150>  61/167225
<151>  2009-04-07

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  38
<212>  RNA
<213>  artificial sequence

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(38)
<223>  all purines (A and G) are ribonucleotides (2'-OH), all 
       pyrimidines (C and U) are 2'-fluorinated nucleotides

<400>  1
cucaacgagc caggaacauc gacgucagca aacgcgag                               38


<210>  2
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(38)
<223>  all purines (A and G) are ribonucleotides (2'-OH), except at 
       positions 7, 8, 9, 13, 14, 15, 18, 21, 22, 24, 27, 28 and 31, 
       which are 2'-O-methyl modified nucleotides

<220>
<221>  misc_feature
<222>  (1)..(38)
<223>  all pyrimidines (C and U) are 2'-fluorinated nucleotides

<220>
<221>  misc_feature
<222>  (39)..(39)
<223>  T at position 39 is an inverted deoxy thymidine

<400>  2
cucaacgagc caggaacauc gacgucagca aacgcgagt                              39


<210>  3
<211>  40
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n at position 1 is a 5'-hexylamine linker phosphoramidite

<220>
<221>  misc_feature
<222>  (2)..(39)
<223>  all purines (A and G) are ribonucleotides (2'-OH), except at 
       positions 8, 9, 10, 14, 15, 16, 19, 22, 23, 25, 28, 29 and 32, 
       which are 2'-O-methyl modified nucleotides

<220>
<221>  misc_feature
<222>  (2)..(39)
<223>  all pyrimidines (C and U) are 2'-fluorinated nucleotides

<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  T at position 40 is an inverted deoxy thymidine

<400>  3
ncucaacgag ccaggaacau cgacgucagc aaacgcgagt                             40


<210>  4
<211>  41
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n at position 1 is a 40kDa polyethylene glycol moiety

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n at position 2 is an amine linker

<220>
<221>  misc_feature
<222>  (3)..(40)
<223>  all purines (A and G) are ribonucleotides (2'-OH), except at 
       positions 9, 10, 11, 15, 16, 17, 20, 23, 24, 26, 29, 30 and 33, 
       which are 2'-O-methyl modified nucleotides

<220>
<221>  misc_feature
<222>  (3)..(40)
<223>  all pyrimidines (C and U) are 2'-fluorinated nucleotides

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  T at position 41 is an inverted deoxy thymidine

<400>  4
nncucaacga gccaggaaca ucgacgucag caaacgcgag t                           41


<210>  5
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n at position 1 is a 40 kDa polyethylene glycol moiety

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n at position 2 is an amine linker

<220>
<221>  misc_feature
<222>  (3)..(34)
<223>  all purines (A and G) are 2'-O-methyl modified nucleotides; all 
       pyrimidines (C and U) are 2'-fluorinated nucleotides

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  T at positoin 35 is an inverted deoxy thymidine

<400>  5
nncucgcaga caaccggaug aaauccgacc ggagt                                  35


<210>  6
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n at position 1 is a 40 kDa polyethylene glycol moiety

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n at position 2 is an amine linker

<220>
<221>  misc_feature
<222>  (3)..(34)
<223>  all purines (A and G) are 2'-O-methyl modified nucleotides; all 
       pyrimidines (C and U) are 2'-fluorinated nucleotides

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  T at position 35 is an inverted deoxy thymidine

<400>  6
nncuccagac acagcggaug aaauccggcc agagt                                  35


<210>  7
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  7

Ser Phe Leu Leu Arg Asn Pro 
1               5           


<210>  8
<211>  4
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  8

Gly Pro Arg Pro 
1               


<210>  9
<211>  10
<212>  DNA
<213>  artificial sequence

<220>
<223>  CpG motif

<400>  9
aacgttcgag                                                              10


