
                                SEQUENCE LISTING

<110> Pioneer Hi-Bred International, Inc.
      E. I. DuPont de Nemours & Co.

<120> Novel Bacillus Thuringiensis Gene with
  Lepidopteran Activity

<130> 2573-PCT

<150> 61/053,274          
<151> 2008-05-15  

<160> 4

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 2136
<212> DNA
<213> Bacillus thuringiensis

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> GS018

<400> 1
atggcttata ataataatca aaatcaatgc ataccttata attgtttgaa taatcccgaa 60
atcgaaatat tagaaggcgg aagaatatca gttggtaata ccccaattga tatttctctt 120
tcgcttactc agtttctttt gagtgaattt gtcccaggtg cggggtttgt attaggatta 180
attgatttaa tatggggatt tgtaggtcct tcccaatggg acgcatttct tgctcaagtg 240
gaacagttaa ttaaccaaag aatagcagaa gctgtaagaa atacagcaat tcaggaatta 300
gagggaatgg cacgggttta tagaacctat gctactgctt ttgctgagtg ggaaaaagct 360
cctgatgacc cagagctaag agaagcacta cgtacacaat ttacagcaac tgagacttat 420
ataagtggaa gaatatccgt tttaaaaatt caaacttttg aagtacagct gttatcagtg 480
tttgcccaag ctgcaaattt acatttatct ttattaagag acgttgtgtt ttttgggcaa 540
agatggggtt tttcaacgac aaccgtaaat aattactaca atgatttaac agaagggatt 600
agtacctata cagattatgc tgtacgctgg tacaatacgg gattagaacg tgtatgggga 660
ccggattcta gagattgggt aaggtataat caatttagaa gagaattaac actaactgta 720
ttagatatcg ttgctctgtt cccgaattat gatagtagaa gatatccaat tcgaacagtt 780
tcccaattaa caagagaaat ttatacaaac ccagtattag aaaattttga tggtagtttt 840
cgaggctcgg ctcagggcat agaaagaagt attaggagtc cacatttgat ggatatactt 900
aacagtataa ccatctatac ggatgctcat aggggttatt attattggtc agggcatcaa 960
ataatggctt ctcctgtcgg tttttcgggg ccagaattca cgtttccgct atatggaacc 1020
atgggaaatg cagctccaca acaacgtatt gttgcccaac taggtcaggg cgtgtataga 1080
acattatcct ctacttttta tagaagacct tttaatatag ggataaataa tcaacaacta 1140
tctgttcttg acgggacaga atttgcttat ggaacctcct caaatttgcc atccgctgta 1200
tacagaaaaa gcggaacggt agattcgctg gatgaaatac caccacagaa taacaacgtg 1260
ccacctaggc aaggatttag tcatcgatta agccatgttt caatgtttcg ttcaggctct 1320
agtagtagtg taagtataat aagagctcct atgttctctt ggatacatcg tagtgctgaa 1380
tttaataata taattgcatc ggatagtatt actcaaatcc ctgcagtgaa gggaaacttt 1440
ctttttaatg gttctgtaat ttcaggacca ggatttactg gtggggactt agttagatta 1500
aatagtagtg gaaataacat tcagaataga gggtatattg aagttccaat tcacttccca 1560
tcgacatcta ccagatatcg agttcgtgta cggtatgctt ctgtaacccc gattcacctc 1620
aacgttaatt ggggtaattc atccattttt tccaatacag taccagctac agctacgtca 1680
ttagataatc tacaatcaag tgattttggt tattttgaaa gtgccaatgc ttttacatct 1740
tcattaggta atatagtagg tgttagaaat tttagtggga ctgcaggagt gataatagac 1800
agatttgaat ttattccagt tactgcaaca ctcgaggctg aatataatct ggagagagcg 1860
cagaaggcgg tggatgcgct gtttacgtct acagaccaac tagggctaaa aacaaatgta 1920
acggattatc atattgatca agtgtccaat ttagttacgt gtttatcgga tgaatttggt 1980
ctggatgaaa agcgagaatt gtccgagaaa gtcaaacatg cgaagcgact cagtgatgaa 2040
cgcaatttac tccaagattc aaatttcaaa gacattaata ggcaaccaga acgtgggtgg 2100
ggcggaatta ctccttatgg aggaattagc ggctag                           2136

<210> 2
<211> 711
<212> PRT
<213> Bacillus thuringiensis

<220> 
<221> PEPTIDE        
<222> (0)...(0)
<223> GS018

<400> 2
Met Ala Tyr Asn Asn Asn Gln Asn Gln Cys Ile Pro Tyr Asn Cys Leu
 1               5                  10                  15      
Asn Asn Pro Glu Ile Glu Ile Leu Glu Gly Gly Arg Ile Ser Val Gly
            20                  25                  30          
Asn Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
        35                  40                  45              
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Ile Asp Leu Ile
    50                  55                  60                  
Trp Gly Phe Val Gly Pro Ser Gln Trp Asp Ala Phe Leu Ala Gln Val
65                  70                  75                  80  
Glu Gln Leu Ile Asn Gln Arg Ile Ala Glu Ala Val Arg Asn Thr Ala
                85                  90                  95      
Ile Gln Glu Leu Glu Gly Met Ala Arg Val Tyr Arg Thr Tyr Ala Thr
            100                 105                 110         
Ala Phe Ala Glu Trp Glu Lys Ala Pro Asp Asp Pro Glu Leu Arg Glu
        115                 120                 125             
Ala Leu Arg Thr Gln Phe Thr Ala Thr Glu Thr Tyr Ile Ser Gly Arg
    130                 135                 140                 
Ile Ser Val Leu Lys Ile Gln Thr Phe Glu Val Gln Leu Leu Ser Val
145                 150                 155                 160 
Phe Ala Gln Ala Ala Asn Leu His Leu Ser Leu Leu Arg Asp Val Val
                165                 170                 175     
Phe Phe Gly Gln Arg Trp Gly Phe Ser Thr Thr Thr Val Asn Asn Tyr
            180                 185                 190         
Tyr Asn Asp Leu Thr Glu Gly Ile Ser Thr Tyr Thr Asp Tyr Ala Val
        195                 200                 205             
Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
    210                 215                 220                 
Asp Trp Val Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val
225                 230                 235                 240 
Leu Asp Ile Val Ala Leu Phe Pro Asn Tyr Asp Ser Arg Arg Tyr Pro
                245                 250                 255     
Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val
            260                 265                 270         
Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu
        275                 280                 285             
Arg Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr
    290                 295                 300                 
Ile Tyr Thr Asp Ala His Arg Gly Tyr Tyr Tyr Trp Ser Gly His Gln
305                 310                 315                 320 
Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
                325                 330                 335     
Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala
            340                 345                 350         
Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Phe Tyr Arg
        355                 360                 365             
Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp
    370                 375                 380                 
Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val
385                 390                 395                 400 
Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln
                405                 410                 415     
Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His
            420                 425                 430         
Val Ser Met Phe Arg Ser Gly Ser Ser Ser Ser Val Ser Ile Ile Arg
        435                 440                 445             
Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn Ile
    450                 455                 460                 
Ile Ala Ser Asp Ser Ile Thr Gln Ile Pro Ala Val Lys Gly Asn Phe
465                 470                 475                 480 
Leu Phe Asn Gly Ser Val Ile Ser Gly Pro Gly Phe Thr Gly Gly Asp
                485                 490                 495     
Leu Val Arg Leu Asn Ser Ser Gly Asn Asn Ile Gln Asn Arg Gly Tyr
            500                 505                 510         
Ile Glu Val Pro Ile His Phe Pro Ser Thr Ser Thr Arg Tyr Arg Val
        515                 520                 525             
Arg Val Arg Tyr Ala Ser Val Thr Pro Ile His Leu Asn Val Asn Trp
    530                 535                 540                 
Gly Asn Ser Ser Ile Phe Ser Asn Thr Val Pro Ala Thr Ala Thr Ser
545                 550                 555                 560 
Leu Asp Asn Leu Gln Ser Ser Asp Phe Gly Tyr Phe Glu Ser Ala Asn
                565                 570                 575     
Ala Phe Thr Ser Ser Leu Gly Asn Ile Val Gly Val Arg Asn Phe Ser
            580                 585                 590         
Gly Thr Ala Gly Val Ile Ile Asp Arg Phe Glu Phe Ile Pro Val Thr
        595                 600                 605             
Ala Thr Leu Glu Ala Glu Tyr Asn Leu Glu Arg Ala Gln Lys Ala Val
    610                 615                 620                 
Asp Ala Leu Phe Thr Ser Thr Asp Gln Leu Gly Leu Lys Thr Asn Val
625                 630                 635                 640 
Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Thr Cys Leu Ser
                645                 650                 655     
Asp Glu Phe Gly Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys
            660                 665                 670         
His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Ser Asn
        675                 680                 685             
Phe Lys Asp Ile Asn Arg Gln Pro Glu Arg Gly Trp Gly Gly Ile Thr
    690                 695                 700                 
Pro Tyr Gly Gly Ile Ser Gly
705                 710     


<210> 3
<211> 2139
<212> DNA
<213> Artificial Sequence

<220> 
<223> Unknown

<400> 3
atgccttata ataataatca aaatcaatgc ataccttata attgtttgaa taatcccgaa 60
atcgaaatat tagaaggcgg aagaatatca gttggtaata ccccaattga tatttctctt 120
tcgcttactc agtttctttt gagtgaattt gtcccaggtg cggggtttgt attaggatta 180
attgatttaa tatggggatt tgtaggtcct tcccaatggg acgcatttct tgctcaagtg 240
gaacagttaa ttaaccaaag aatagcagaa gctgtaagaa atacagcaat tcaggaatta 300
gagggaatgg cacgggttta tagaacctat gctactgctt ttgctgagtg ggaaaaagct 360
cctgatgacc cagagctaag agaagcacta cgtacacaat ttacagcaac tgagacttat 420
ataagtggaa gaatatccgt tttaaaaatt caaacttttg aagtacagct gttatcagtg 480
tttgcccaag ctgcaaattt acatttatct ttattaagag acgttgtgtt ttttgggcaa 540
agatggggtt tttcaacgac aaccgtaaat aattactaca atgatttaac agaagggatt 600
agtacctata cagattatgc tgtacgctgg tacaatacgg gattagaacg tgtatgggga 660
ccggattcta gagattgggt aaggtataat caatttagaa gagaattaac actaactgta 720
ttagatatcg ttgctctgtt cccgaattat gatagtagaa gatatccaat tcgaacagtt 780
tcccaattaa caagagaaat ttatacaaac ccagtattag aaaattttga tggtagtttt 840
cgaggctcgg ctcagggcat agaaagaagt attaggagtc cacatttgat ggatatactt 900
aacagtataa ccatctatac ggatgctcat aggggttatt attattggtc agggcatcaa 960
ataatggctt ctcctgtcgg tttttcgggg ccagaattca cgtttccgct atatggaacc 1020
atgggaaatg cagctccaca acaacgtatt gttgcccaac taggtcaggg cgtgtataga 1080
acattatcct ctacttttta tagaagacct tttaatatag ggataaataa tcaacaacta 1140
tctgttcttg acgggacaga atttgcttat ggaacctcct caaatttgcc atccgctgta 1200
tacagaaaaa gcggaacggt agattcgctg gatgaaatac caccacagaa taacaacgtg 1260
ccacctaggc aaggatttag tcatcgatta agccatgttt caatgtttcg ttcaggcttt 1320
agtaatagta gtgtaagtat aataagagct cctatgttct cttggataca tcgtagtgct 1380
gaatttaata atataattgc atcggatagt attactcaaa tccctgcagt gaagggaaac 1440
tttcttttta atggttctgt aatttcagga ccaggattta ctggtgggga cttagttaga 1500
ttaaatagta gtggaaataa cattcagaat agagggtata ttgaagttcc aattcacttc 1560
ccatcgacat ctaccagata tcgagttcgt gtacggtatg cttctgtaac cccgattcac 1620
ctcaacgtta attggggtaa ttcatccatt ttttccaata cagtaccagc tacagctacg 1680
tcattagata atctacaatc aagtgatttt ggttattttg aaagtgccaa tgcttttaca 1740
tcttcattag gtaatatagt aggtgttaga aattttagtg ggactgcagg agtgataata 1800
gacagatttg aatttattcc agttactgca acactcgagg ctgaatataa tctggagaga 1860
gcgcagaagg cggtggatgc gctgtttacg tctacagacc aactagggct aaaaacaaat 1920
gtaacggatt atcatattga tcaagtgtcc aatttagtta cgtgtttatc ggatgaattt 1980
ggtctggatg aaaagcgaga attgtccgag aaagtcaaac atgcgaagcg actcagtgat 2040
gaacgcaatt tactccaaga ttcaaatttc aaagacatta ataggcaacc agaacgtggg 2100
tggggcggaa ttactcctta tggaggaatt agcggctag                        2139

<210> 4
<211> 712
<212> PRT
<213> Artificial Sequence

<220> 
<223> Unknown

<400> 4
Met Pro Tyr Asn Asn Asn Gln Asn Gln Cys Ile Pro Tyr Asn Cys Leu
 1               5                  10                  15      
Asn Asn Pro Glu Ile Glu Ile Leu Glu Gly Gly Arg Ile Ser Val Gly
            20                  25                  30          
Asn Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
        35                  40                  45              
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Ile Asp Leu Ile
    50                  55                  60                  
Trp Gly Phe Val Gly Pro Ser Gln Trp Asp Ala Phe Leu Ala Gln Val
65                  70                  75                  80  
Glu Gln Leu Ile Asn Gln Arg Ile Ala Glu Ala Val Arg Asn Thr Ala
                85                  90                  95      
Ile Gln Glu Leu Glu Gly Met Ala Arg Val Tyr Arg Thr Tyr Ala Thr
            100                 105                 110         
Ala Phe Ala Glu Trp Glu Lys Ala Pro Asp Asp Pro Glu Leu Arg Glu
        115                 120                 125             
Ala Leu Arg Thr Gln Phe Thr Ala Thr Glu Thr Tyr Ile Ser Gly Arg
    130                 135                 140                 
Ile Ser Val Leu Lys Ile Gln Thr Phe Glu Val Gln Leu Leu Ser Val
145                 150                 155                 160 
Phe Ala Gln Ala Ala Asn Leu His Leu Ser Leu Leu Arg Asp Val Val
                165                 170                 175     
Phe Phe Gly Gln Arg Trp Gly Phe Ser Thr Thr Thr Val Asn Asn Tyr
            180                 185                 190         
Tyr Asn Asp Leu Thr Glu Gly Ile Ser Thr Tyr Thr Asp Tyr Ala Val
        195                 200                 205             
Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
    210                 215                 220                 
Asp Trp Val Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val
225                 230                 235                 240 
Leu Asp Ile Val Ala Leu Phe Pro Asn Tyr Asp Ser Arg Arg Tyr Pro
                245                 250                 255     
Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val
            260                 265                 270         
Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu
        275                 280                 285             
Arg Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr
    290                 295                 300                 
Ile Tyr Thr Asp Ala His Arg Gly Tyr Tyr Tyr Trp Ser Gly His Gln
305                 310                 315                 320 
Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
                325                 330                 335     
Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala
            340                 345                 350         
Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Phe Tyr Arg
        355                 360                 365             
Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp
    370                 375                 380                 
Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val
385                 390                 395                 400 
Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln
                405                 410                 415     
Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His
            420                 425                 430         
Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
        435                 440                 445             
Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
    450                 455                 460                 
Ile Ile Ala Ser Asp Ser Ile Thr Gln Ile Pro Ala Val Lys Gly Asn
465                 470                 475                 480 
Phe Leu Phe Asn Gly Ser Val Ile Ser Gly Pro Gly Phe Thr Gly Gly
                485                 490                 495     
Asp Leu Val Arg Leu Asn Ser Ser Gly Asn Asn Ile Gln Asn Arg Gly
            500                 505                 510         
Tyr Ile Glu Val Pro Ile His Phe Pro Ser Thr Ser Thr Arg Tyr Arg
        515                 520                 525             
Val Arg Val Arg Tyr Ala Ser Val Thr Pro Ile His Leu Asn Val Asn
    530                 535                 540                 
Trp Gly Asn Ser Ser Ile Phe Ser Asn Thr Val Pro Ala Thr Ala Thr
545                 550                 555                 560 
Ser Leu Asp Asn Leu Gln Ser Ser Asp Phe Gly Tyr Phe Glu Ser Ala
                565                 570                 575     
Asn Ala Phe Thr Ser Ser Leu Gly Asn Ile Val Gly Val Arg Asn Phe
            580                 585                 590         
Ser Gly Thr Ala Gly Val Ile Ile Asp Arg Phe Glu Phe Ile Pro Val
        595                 600                 605             
Thr Ala Thr Leu Glu Ala Glu Tyr Asn Leu Glu Arg Ala Gln Lys Ala
    610                 615                 620                 
Val Asp Ala Leu Phe Thr Ser Thr Asp Gln Leu Gly Leu Lys Thr Asn
625                 630                 635                 640 
Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Thr Cys Leu
                645                 650                 655     
Ser Asp Glu Phe Gly Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val
            660                 665                 670         
Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Ser
        675                 680                 685             
Asn Phe Lys Asp Ile Asn Arg Gln Pro Glu Arg Gly Trp Gly Gly Ile
    690                 695                 700                 
Thr Pro Tyr Gly Gly Ile Ser Gly
705                 710         

