                         SEQUENCE LISTING

<110>  Bayer HealthCare LLC
       Brooks, Alan
       Murphy, John E.
       Seto, Marian
       Jiang, Xiaoqiao
       Kiewlich, David
       Patel, Chandra
 
<120>  SITE-DIRECTED MODIFICATION OF FACTOR IX

<130>  MSB-7323

<150>  US 61/124,568
<151>  2008-04-16

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  415
<212>  PRT
<213>  Homo sapiens

<400>  1

Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 
1               5                   10                  15      


Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 
            20                  25                  30          


Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 
        35                  40                  45              


Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 
    50                  55                  60                  


Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 
65                  70                  75                  80  


Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 
                85                  90                  95      


Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 
            100                 105                 110         


Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 
        115                 120                 125             


Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 
    130                 135                 140                 


Arg Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 
145                 150                 155                 160 


Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 
                165                 170                 175     


Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 
            180                 185                 190         


Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 
        195                 200                 205             


Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 
    210                 215                 220                 


Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 
225                 230                 235                 240 


Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 
                245                 250                 255     


His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 
            260                 265                 270         


Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 
        275                 280                 285             


Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 
    290                 295                 300                 


Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 
305                 310                 315                 320 


Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 
                325                 330                 335     


Leu Arg Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 
            340                 345                 350         


Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 
        355                 360                 365             


His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 
    370                 375                 380                 


Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 
385                 390                 395                 400 


Val Ser Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr Tyr 
                405                 410                 415 1   


Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg Glu 
            5                   10                  15          


Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe Glu 
        20                  25                  30              


Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly Asp 
    35                  40                  45                  


Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp Asp 
50                  55                  60                  65  


Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys Asn 
                70                  75                  80      


Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu Gln 
            85                  90                  95          


Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr Glu 
        100                 105                 110             


Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val Pro 
    115                 120                 125                 


Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr Arg 
130                 135                 140                 145 


Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu Ala 
                150                 155                 160     


Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn Asp 
            165                 170                 175         


Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe Pro 
        180                 185                 190             


Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly Ser 
    195                 200                 205                 


Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu Thr 
210                 215                 220                 225 


Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu Thr 
                230                 235                 240     


Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His His 
            245                 250                 255         


Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu Leu 
        260                 265                 270             


Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile Cys 
    275                 280                 285                 


Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser Gly 
290                 295                 300                 305 


Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala Leu 
                310                 315                 320     


Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys Leu 
            325                 330                 335         


Arg Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly Phe 
        340                 345                 350             


His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro His 
    355                 360                 365                 


Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser Trp 
370                 375                 380                 385 


Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys Val 
                390                 395                 400     


Ser Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr 
            405                 410                 415 


<210>  2
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' primer FIXF1

<400>  2
atcataagct tgccaccatg cagcgcgtga acatg                                  35


<210>  3
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' primer FIXR3

<400>  3
atcataagct tgattagtta gtgagaggcc ctg                                    33


<210>  4
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - K201C

<400>  4
tggcaggttg ttttgaatgg ttgcgttgat gcattctgtg gaggc                       45


<210>  5
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - K247C

<400>  5
gaacataata ttgaggagac agaacataca gagcaatgcc gaaatgtgat tcgaatta         58


<210>  6
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - K392C

<400>  6
tagctggggt gaagagtgtg caatgtgcgg caaatatgga atatatacc                   49


<210>  7
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - K413C

<400>  7
gtatgtcaac tggattaagg aaaaaacatg cctcacttaa tgaaagatgg atttccaa         58


<210>  8
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - L414C

<400>  8
tcaactggat taaggaaaaa acaaagtgca cttaatgaaa gatggatttc caag             54


<210>  9
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - T415C

<400>  9
ggattaagga aaaaacaaag ctttgttaat gaaagatgga tttccaagg                   49


<210>  10
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - T148C

<400>  10
agctcacccg tgctgagtgt gtttttcctg atgtgg                                 36


<210>  11
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - V153C

<400>  11
cccgtgctga gactgttttt cctgattgcg actatgtaaa ttctactga                   49


<210>  12
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - T163C

<400>  12
ctatgtaaat tctactgaag ctgaatgcat tttggataac atcactcaaa gc               52


<210>  13
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - L165C

<400>  13
ctactgaagc tgaaaccatt tgcgataaca tcactcaaag cacc                        44


<210>  14
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - N167C

<400>  14
gaagctgaaa ccattttgga ttgcatcact caaagcaccc aatc                        44


<210>  15
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - T169C

<400>  15
gctgaaacca ttttggataa catctgtcaa agcacccaat catttaatga                  50


<210>  16
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - T172C

<400>  16
attttggata acatcactca aagctgccaa tcatttaatg acttcactcg                  50


<210>  17
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Strand Oligonucleotide Sequence - F175C

<400>  17
ctcaaagcac ccaatcatgt aatgacttca ctcgggt                                37


<210>  18
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide - CF1

<400>  18
gtaaattcta ctgaggcctg cgaaaccatt                                        30


<210>  19
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide - CR1

<400>  19
catttaagat gactccggac gctttggtaa gatc                                   34


<210>  20
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  20
gttgaccgag ccacatgcct tgcatctaca aagttcacca tc                          42


<210>  21
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  21
gatggtgaac tttgtagatg caaggcatgt ggctcggtca ac                          42


