                         SEQUENCE LISTING

<110>  Naval Medical Research Center (CODE 00L)
       Ching, Wei-Mei
       Chao, Chien-Chung
 
<120>  RECOMBINANT CHIMERIC ANTIGENS FOR DIAGNOSIS AND PREVENTION OF 
       SCRUB TYPHUS

<130>  NC

<160>  5     

<170>  PatentIn version 3.4

<210>  1
<211>  377
<212>  PRT
<213>  Orientia tsutsugamushi


<220>
<221>  MISC_FEATURE
<223>  Chimeric protein 1 based on sequences of recombinant r56 protein 
       of multiple stains

<400>  1

Met Thr Ile Thr Pro Ser Phe Arg Ala Glu Leu Gly Val Met Tyr Leu 
1               5                   10                  15      


Arg Asn Ile Thr Ala Gln Val Glu Glu Gly Lys Val Lys Ala Asp Ser 
            20                  25                  30          


Gly Ser Lys Thr Lys Ala Asp Ser Gly Gly Glu Thr Asp Ala Pro Ile 
        35                  40                  45              


Arg Lys Arg Phe Lys Leu Thr Pro Pro Gln Pro Thr Ile Met Pro Ile 
    50                  55                  60                  


Ser Ile Ala Asp Arg Asp Phe Gly Ile Asp Ile Pro Asn Ile Pro Gln 
65                  70                  75                  80  


Gln Gln Ala Gln Ala Ala Gln Pro Gln Leu Asn Asp Glu Gln Arg Ala 
                85                  90                  95      


Ala Ala Arg Ile Ala Trp Leu Lys Asn Cys Ala Gly Ile Asp Tyr Arg 
            100                 105                 110         


Val Lys Asn Pro Asn Asp Pro Asn Gly Pro Met Val Ile Asn Pro Ile 
        115                 120                 125             


Leu Leu Asn Ile Pro Gln Gly Asn Pro Asn Pro Val Gly Asn Pro Pro 
    130                 135                 140                 


Gln Arg Ala Asn Pro Pro Ala Gly Phe Ala Ile His Asn His Glu Gln 
145                 150                 155                 160 


Trp Arg His Leu Val Val Gly Leu Ala Ala Leu Ser Asn Ala Asn Lys 
                165                 170                 175     


Pro Ser Ala Ser Pro Val Lys Val Leu Ser Asp Lys Ile Thr Gln Ile 
            180                 185                 190         


Tyr Ser Asp Ile Lys Pro Phe Ala Asp Ile Ala Gly Ile Asp Val Pro 
        195                 200                 205             


Asp Thr Ser Leu Pro Asn Ser Ala Ser Val Glu Gln Ile Gln Asn Lys 
    210                 215                 220                 


Met Gln Glu Leu Asn Asp Leu Leu Glu Glu Leu Arg Glu Ser Phe Asp 
225                 230                 235                 240 


Gly Tyr Leu Gly Gly Asn Ala Phe Ala Asn Gln Ile Gln Leu Asn Phe 
                245                 250                 255     


Val Met Pro Gln Gln Ala Gln Gln Gln Gly Gln Gly Gln Gln Gln Gln 
            260                 265                 270         


Ala Gln Ala Thr Ala Gln Glu Ala Val Ala Ala Ala Ala Val Arg Leu 
        275                 280                 285             


Leu Asn Gly Asn Asp Gln Ile Ala Gln Leu Tyr Lys Asp Leu Val Lys 
    290                 295                 300                 


Leu Gln Arg His Ala Gly Ile Lys Lys Ala Met Glu Lys Leu Ala Ala 
305                 310                 315                 320 


Gln Gln Glu Glu Asp Ala Lys Asn Gln Gly Glu Gly Asp Cys Lys Gln 
                325                 330                 335     


Gln Gln Gly Thr Ser Glu Lys Ser Lys Lys Gly Lys Asp Lys Glu Ala 
            340                 345                 350         


Glu Phe Asp Leu Ser Met Ile Val Gly Gln Val Lys Leu Tyr Ala Asp 
        355                 360                 365             


Val Met Ile Thr Glu Ser Val Ser Ile 
    370                 375         


<210>  2
<211>  377
<212>  PRT
<213>  Orientia tsutsugamushi


<220>
<221>  MISC_FEATURE
<223>  Chimeric protein 2 based on sequence of recombinant r56 proteins 
       from multiple strains

<400>  2

Met Thr Ile Thr Pro Ser Ile Arg Ala Glu Leu Gly Val Met Tyr Leu 
1               5                   10                  15      


Arg Asn Ile Ser Ala Glu Val Glu Leu Gly Lys Val Lys Ala Asp Ser 
            20                  25                  30          


Gly Ser Lys Thr Lys Ala Asp Ser Gly Gly Glu Thr Asp Ala Pro Ile 
        35                  40                  45              


Arg Lys Arg Phe Lys Leu Thr Pro Pro Gln Pro Thr Ile Met Pro Ile 
    50                  55                  60                  


Ser Ile Ala Asp Arg Asp Phe Gly Val Asp Val Thr Asn Ile Pro Gln 
65                  70                  75                  80  


Ala Gln Val Gln Pro Pro Gln Gln Ala Asn Asp Pro Leu Val Arg Gly 
                85                  90                  95      


Val Arg Arg Ile Ala Trp Leu Lys Glu Cys Ala Gly Ile Asp Tyr Met 
            100                 105                 110         


Val Lys Asp Pro Asn Asn Pro Asn Gly Pro Met Met Ile Asn Pro Ile 
        115                 120                 125             


Leu Leu Asn Ile Pro Gln Gly Asn Pro Asn Ala Gln Gly Asn Pro Pro 
    130                 135                 140                 


Gln Arg Ala Asn Pro Pro Ala Gly Phe Asn Ile His Asn His Glu Gln 
145                 150                 155                 160 


Trp Arg His Phe Val Val Gly Leu Ala Ala Leu Ser Asn Ala Asn Lys 
                165                 170                 175     


Pro Ser Ala Ser Pro Val Lys Val Leu Ser Asp Lys Ile Thr Gln Ile 
            180                 185                 190         


Tyr Ser Asp Ile Lys Pro Phe Ala Asp Ile Ala Gly Ile Asp Val Pro 
        195                 200                 205             


Asp Thr Ser Leu Pro Asn Ser Ala Ser Val Glu Gln Ile Gln Asn Lys 
    210                 215                 220                 


Met Gln Glu Leu Asn Asp Leu Leu Glu Glu Leu Arg Glu Ser Phe Asp 
225                 230                 235                 240 


Gly Tyr Leu Gly Gly Asn Ala Phe Ala Asn Gln Ile Gln Leu Asn Phe 
                245                 250                 255     


Val Met Pro Gln Gln Ala Gln Gln Gln Gly Gln Gly Gln Gln Gln Gln 
            260                 265                 270         


Ala Gln Ala Thr Ala Gln Glu Ala Val Ala Ala Ala Ala Val Arg Leu 
        275                 280                 285             


Leu Asn Gly Asn Asp Gln Ile Ala Gln Leu Tyr Lys Asp Leu Val Lys 
    290                 295                 300                 


Leu Gln Arg His Ala Gly Ile Lys Lys Ala Met Glu Lys Leu Ala Ala 
305                 310                 315                 320 


Gln Gln Glu Glu Asp Ala Lys Asn Gln Gly Glu Gly Asp Cys Lys Gln 
                325                 330                 335     


Gln Gln Gly Thr Ser Glu Lys Ser Lys Glu Gly Ser Lys Lys Glu Pro 
            340                 345                 350         


Glu Phe Asp Leu Ser Met Ile Val Gly Gln Val Lys Leu Tyr Ala Asp 
        355                 360                 365             


Val Met Ile Thr Glu Ser Val Ser Ile 
    370                 375         


<210>  3
<211>  377
<212>  PRT
<213>  Orientia tsutsugamushi


<220>
<221>  MISC_FEATURE
<223>  Chimeric protein 3 based on sequences of recombinant r56 proteins
       from multiple stains

<400>  3

Met Thr Ile Thr Pro Ser Phe Arg Ala Glu Leu Gly Val Met Tyr Leu 
1               5                   10                  15      


Arg Asn Ile Thr Ala Gln Val Glu Glu Gly Lys Val Lys Ala Asp Ser 
            20                  25                  30          


Gly Ser Lys Thr Lys Ala Asp Ser Gly Gly Glu Thr Asp Ala Pro Ile 
        35                  40                  45              


Arg Lys Arg Phe Lys Leu Thr Pro Pro Gln Pro Thr Ile Met Pro Ile 
    50                  55                  60                  


Ser Ile Ala Asp Arg Asp Phe Gly Val Asp Val Pro Asn Ile Pro Gln 
65                  70                  75                  80  


Ala Gln Val Gln Pro Pro Gln Gln Ala Asn Asp Pro Leu Gln Arg Gly 
                85                  90                  95      


Val Arg Arg Ile Ala Trp Leu Lys Asn Cys Ala Gly Ile Asp Tyr Met 
            100                 105                 110         


Val Lys Asp Pro Asn Asp Pro Asn Gly Pro Met Met Ile Asn Pro Ile 
        115                 120                 125             


Leu Leu Asn Ile Pro Gln Gly Asn Pro Asn Ala Gln Gly Asn Pro Pro 
    130                 135                 140                 


Gln Arg Ala Asn Pro Pro Ala Gly Phe Asn Ile His Asn His Glu Gln 
145                 150                 155                 160 


Trp Arg His Phe Val Val Gly Leu Ala Ala Leu Ser Asn Ala Asn Lys 
                165                 170                 175     


Pro Ser Ala Ser Pro Val Lys Val Leu Ser Asp Lys Ile Thr Gln Ile 
            180                 185                 190         


Tyr Ser Asp Ile Lys Pro Phe Ala Asp Ile Ala Gly Ile Asp Val Pro 
        195                 200                 205             


Asp Thr Ser Leu Pro Asn Ser Ala Ser Val Glu Gln Ile Gln Asn Lys 
    210                 215                 220                 


Met Gln Glu Leu Asn Asp Leu Leu Glu Glu Leu Arg Glu Ser Phe Asp 
225                 230                 235                 240 


Gly Tyr Leu Gly Gly Asn Ala Phe Ala Asn Gln Ile Gln Leu Asn Phe 
                245                 250                 255     


Val Met Pro Gln Gln Ala Gln Gln Gln Gly Gln Gly Gln Gln Gln Gln 
            260                 265                 270         


Ala Gln Ala Thr Ala Gln Glu Ala Val Ala Ala Ala Ala Val Arg Leu 
        275                 280                 285             


Leu Asn Gly Asn Asp Gln Ile Ala Gln Leu Tyr Lys Asp Leu Val Lys 
    290                 295                 300                 


Leu Gln Arg His Ala Gly Ile Lys Lys Ala Met Glu Lys Leu Ala Ala 
305                 310                 315                 320 


Gln Gln Glu Glu Asp Ala Lys Asn Gln Gly Glu Gly Asp Cys Lys Gln 
                325                 330                 335     


Gln Gln Gly Thr Ser Glu Lys Ser Lys Lys Gly Lys Asp Lys Glu Ala 
            340                 345                 350         


Glu Phe Asp Leu Ser Met Ile Val Gly Gln Val Lys Leu Tyr Ala Asp 
        355                 360                 365             


Val Met Ile Thr Glu Ser Val Ser Ile 
    370                 375         


<210>  4
<211>  370
<212>  PRT
<213>  Orientia tsutsugamushi


<220>
<221>  MISC_FEATURE
<223>  TA763r56 Cloning sequence

<400>  4

Met Thr Ile Thr Pro Ser Ile Arg Ala Glu Leu Gly Val Met Tyr Leu 
1               5                   10                  15      


Arg Asn Ile Ser Ala Glu Val Glu Leu Gly Lys Val Lys Ala Asp Ser 
            20                  25                  30          


Gly Ser Lys Thr Lys Ala Asp Ser Gly Gly Glu Thr Asp Ala Pro Ile 
        35                  40                  45              


Arg Lys Arg Phe Lys Leu Thr Pro Pro Gln Pro Thr Ile Met Pro Ile 
    50                  55                  60                  


Ser Ile Ala Asp Arg Asp Phe Gly Val Asp Val Thr Asn Ile Pro Gln 
65                  70                  75                  80  


Ala Gln Val Gln Pro Pro Gln Gln Ala Asn Asp Pro Leu Val Arg Gly 
                85                  90                  95      


Val Arg Arg Ile Ala Trp Leu Lys Glu Tyr Ala Gly Ile Asp Tyr Met 
            100                 105                 110         


Val Lys Asp Pro Asn Asn Pro Gly Arg Met Met Val Asn Pro Val Leu 
        115                 120                 125             


Leu Asn Ile Pro Gln Gly Pro Pro Ala Gln Asn Pro Arg Ala Ala Met 
    130                 135                 140                 


Gln Pro Cys Asn Ile Leu Asp His Asp His Trp Lys His Phe Val Val 
145                 150                 155                 160 


Gly Val Thr Ala Leu Ser Asn Ala Asn Lys Pro Ser Ala Ser Pro Val 
                165                 170                 175     


Lys Ile Leu Ser Glu Lys Ile Thr Gln Ile Tyr Ser Asp Ile Arg Pro 
            180                 185                 190         


Phe Ala Asp Ile Ala Gly Ile Asp Val Pro Asp Ala Gly Leu Pro Asn 
        195                 200                 205             


Ser Ala Thr Val Glu Gln Ile Gln Asn Lys Met Gln Glu Leu Asn Asp 
    210                 215                 220                 


Val Leu Glu Glu Leu Arg Glu Ser Phe Asp Gly Tyr Leu Gly Gly Asn 
225                 230                 235                 240 


Ala Phe Ala Asn Gln Ile Gln Leu Asn Phe Val Met Pro Gln Gln Ala 
                245                 250                 255     


Gln Gln Gln Gly Gln Gly Gln Gln Gln Gln Ala Gln Ala Thr Ala Gln 
            260                 265                 270         


Glu Ala Val Ala Ala Ala Ala Val Arg Leu Leu Asn Gly Asn Asp Gln 
        275                 280                 285             


Ile Ala Gln Leu Tyr Arg Asp Leu Val Lys Leu Gln Arg His Ala Gly 
    290                 295                 300                 


Ile Lys Lys Ala Met Glu Lys Leu Ala Ala Gln Gln Glu Glu Asp Ala 
305                 310                 315                 320 


Lys Asn Gln Gly Glu Gly Asp Cys Lys Gln Gln Gln Gly Thr Ser Glu 
                325                 330                 335     


Lys Ser Lys Glu Gly Ser Lys Lys Glu Pro Glu Phe Asp Leu Ser Met 
            340                 345                 350         


Ile Val Gly Gln Val Lys Leu Tyr Ala Asp Val Met Ile Thr Glu Ser 
        355                 360                 365             


Val Ser 
    370 


<210>  5
<211>  526
<212>  PRT
<213>  Orientia tsutsugamushi


<220>
<221>  MISC_FEATURE
<223>  Full length 56 kDa from TA763 strain

<400>  5

Met Lys Lys Ile Met Leu Ile Ala Ser Ala Met Ser Ala Leu Ser Leu 
1               5                   10                  15      


Pro Phe Ser Ala Ser Ala Ile Glu Leu Gly Asp Glu Gly Gly Leu Glu 
            20                  25                  30          


Cys Gly Pro Tyr Ala Lys Val Gly Val Ile Gly Gly Met Ile Thr Gly 
        35                  40                  45              


Val Glu Ser Ala Arg Leu Asp Pro Thr Asp Ser Glu Gly Lys Lys His 
    50                  55                  60                  


Leu Ser Leu Thr Thr Gly Met Pro Phe Gly Gly Thr Leu Ala Ala Gly 
65                  70                  75                  80  


Met Thr Ile Thr Pro Ser Ile Arg Ala Glu Leu Gly Val Met Tyr Leu 
                85                  90                  95      


Arg Asn Ile Ser Ala Glu Val Glu Leu Gly Lys Val Lys Ala Asp Ser 
            100                 105                 110         


Gly Ser Lys Thr Lys Ala Asp Ser Gly Gly Glu Thr Asp Ala Pro Ile 
        115                 120                 125             


Arg Lys Arg Phe Lys Leu Thr Pro Pro Gln Pro Thr Ile Met Pro Ile 
    130                 135                 140                 


Ser Ile Ala Asp Arg Asp Phe Gly Val Asp Val Thr Asn Ile Pro Gln 
145                 150                 155                 160 


Ala Gln Val Gln Pro Pro Gln Gln Ala Asn Asp Pro Leu Val Arg Gly 
                165                 170                 175     


Val Arg Arg Ile Ala Trp Leu Lys Glu Tyr Ala Gly Ile Asp Tyr Met 
            180                 185                 190         


Val Lys Asp Pro Asn Asn Pro Gly Arg Met Met Val Asn Pro Val Leu 
        195                 200                 205             


Leu Asn Ile Pro Gln Gly Pro Pro Ala Gln Asn Pro Arg Ala Ala Met 
    210                 215                 220                 


Gln Pro Cys Asn Ile Leu Asp His Asp His Trp Lys His Phe Val Val 
225                 230                 235                 240 


Gly Val Thr Ala Leu Ser Asn Ala Asn Lys Pro Ser Ala Ser Pro Val 
                245                 250                 255     


Lys Ile Leu Ser Glu Lys Ile Thr Gln Ile Tyr Ser Asp Ile Arg Pro 
            260                 265                 270         


Phe Ala Asp Ile Ala Gly Ile Asp Val Pro Asp Ala Gly Leu Pro Asn 
        275                 280                 285             


Ser Ala Thr Val Glu Gln Ile Gln Asn Lys Met Gln Glu Leu Asn Asp 
    290                 295                 300                 


Val Leu Glu Glu Leu Arg Glu Ser Phe Asp Gly Tyr Leu Gly Gly Asn 
305                 310                 315                 320 


Ala Phe Ala Asn Gln Ile Gln Leu Asn Phe Val Met Pro Gln Gln Ala 
                325                 330                 335     


Gln Gln Gln Gly Gln Gly Gln Gln Gln Gln Ala Gln Ala Thr Ala Gln 
            340                 345                 350         


Glu Ala Val Ala Ala Ala Ala Val Arg Leu Leu Asn Gly Asn Asp Gln 
        355                 360                 365             


Ile Ala Gln Leu Tyr Arg Asp Leu Val Lys Leu Gln Arg His Ala Gly 
    370                 375                 380                 


Ile Lys Lys Ala Met Glu Lys Leu Ala Ala Gln Gln Glu Glu Asp Ala 
385                 390                 395                 400 


Lys Asn Gln Gly Glu Gly Asp Cys Lys Gln Gln Gln Gly Thr Ser Glu 
                405                 410                 415     


Lys Ser Lys Glu Gly Ser Lys Lys Glu Pro Glu Phe Asp Leu Ser Met 
            420                 425                 430         


Ile Val Gly Gln Val Lys Leu Tyr Ala Asp Val Met Ile Thr Glu Ser 
        435                 440                 445             


Val Ser Ile Tyr Ala Gly Val Gly Ala Gly Leu Ala Tyr Thr Ser Gly 
    450                 455                 460                 


Lys Ile Asp Asp Lys Asp Thr Lys His Thr Gly Met Val Val Ser Gly 
465                 470                 475                 480 


Ala Leu Gly Val Ala Ile Asn Ala Ala Glu Gly Val Tyr Val Asp Ile 
                485                 490                 495     


Glu Gly Ser Tyr Met Tyr Ser Phe Ser Lys Ile Glu Glu Lys Tyr Ser 
            500                 505                 510         


Ile Asn Pro Leu Met Ala Ser Val Gly Val Arg Tyr Asn Phe 
        515                 520                 525     


