                         SEQUENCE LISTING

<110>  Johnson & Johnson
       Singleton, Kenneth R Mr.
 
<120>  PROTEIN ENGINEERING OF MONOACYLGLYCEROL LIPASE (MGLL)

<130>  PRD2969

<140>  61/047937
<141>  2008-04-25

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  304
<212>  PRT
<213>  Human

<400>  1

Met Pro Glu Glu Ser Ser Pro Arg Arg Thr Pro Gln Ser Ile Pro Tyr 
1               5                   10                  15      


Gln Asp Leu Pro His Leu Val Asn Ala Asp Gly Gln Tyr Leu Phe Cys 
            20                  25                  30          


Arg Tyr Trp Lys Pro Thr Gly Thr Pro Lys Ala Leu Ile Phe Val Ser 
        35                  40                  45              


His Gly Ala Gly Glu His Ser Gly Arg Tyr Glu Glu Leu Ala Arg Met 
    50                  55                  60                  


Leu Met Gly Leu Leu Asp Leu Leu Val Phe Ala His Asp His Val Gly 
65                  70                  75                  80  


His Gly Gln Ser Glu Gly Glu Arg Met Val Val Ser Asp Phe His Val 
                85                  90                  95      


Phe Val Arg Asp Val Leu Gln His Val Asp Ser Met Gln Lys Asp Tyr 
            100                 105                 110         


Pro Gly Leu Pro Val Phe Leu Leu Gly His Ser Met Gly Gly Ala Ile 
        115                 120                 125             


Ala Ile Leu Thr Ala Ala Glu Arg Pro Gly His Phe Ala Gly Met Val 
    130                 135                 140                 


Leu Ile Ser Pro Leu Val Leu Ala Asn Pro Glu Ser Ala Thr Thr Phe 
145                 150                 155                 160 


Lys Val Leu Ala Ala Lys Val Leu Asn Leu Val Leu Pro Asn Leu Ser 
                165                 170                 175     


Leu Gly Pro Ile Asp Ser Ser Val Leu Ser Arg Asn Lys Thr Glu Val 
            180                 185                 190         


Asp Ile Tyr Asn Ser Asp Pro Leu Ile Cys Arg Ala Gly Leu Lys Val 
        195                 200                 205             


Cys Phe Gly Ile Gln Leu Leu Asn Ala Val Ser Arg Val Glu Arg Ala 
    210                 215                 220                 


Leu Pro Lys Leu Thr Val Pro Phe Leu Leu Leu Gln Gly Ser Ala Asp 
225                 230                 235                 240 


Arg Leu Cys Asp Ser Lys Gly Ala Tyr Leu Leu Met Glu Leu Ala Lys 
                245                 250                 255     


Ser Gln Asp Lys Thr Leu Lys Ile Tyr Glu Gly Ala Tyr His Val Leu 
            260                 265                 270         


His Lys Glu Leu Pro Glu Val Thr Asn Ser Val Phe His Glu Ile Asn 
        275                 280                 285             


Met Trp Val Ser Gln Arg Thr Ala Thr Ala Gly Thr Ala Ser Pro Pro 
    290                 295                 300                 


<210>  2
<211>  314
<212>  PRT
<213>  Human

<400>  2

Met Glu Thr Gly Pro Glu Asp Pro Ser Ser Met Pro Glu Glu Ser Ser 
1               5                   10                  15      


Pro Arg Arg Thr Pro Gln Ser Ile Pro Tyr Gln Asp Leu Pro His Leu 
            20                  25                  30          


Val Asn Ala Asp Gly Gln Tyr Leu Phe Cys Arg Tyr Trp Lys Pro Thr 
        35                  40                  45              


Gly Thr Pro Lys Ala Leu Ile Phe Val Ser His Gly Ala Gly Glu His 
    50                  55                  60                  


Ser Gly Arg Tyr Glu Glu Leu Ala Arg Met Leu Met Gly Leu Leu Asp 
65                  70                  75                  80  


Leu Leu Val Phe Ala His Asp His Val Gly His Gly Gln Ser Glu Gly 
                85                  90                  95      


Glu Arg Met Val Val Ser Asp Phe His Val Phe Val Arg Asp Val Leu 
            100                 105                 110         


Gln His Val Asp Ser Met Gln Lys Asp Tyr Pro Gly Leu Pro Val Phe 
        115                 120                 125             


Leu Leu Gly His Ser Met Gly Gly Ala Ile Ala Ile Leu Thr Ala Ala 
    130                 135                 140                 


Glu Arg Pro Gly His Phe Ala Gly Met Val Leu Ile Ser Pro Leu Val 
145                 150                 155                 160 


Leu Ala Asn Pro Glu Ser Ala Thr Thr Phe Lys Val Leu Ala Ala Lys 
                165                 170                 175     


Val Leu Asn Leu Val Leu Pro Asn Leu Ser Leu Gly Pro Ile Asp Ser 
            180                 185                 190         


Ser Val Leu Ser Arg Asn Lys Thr Glu Val Asp Ile Tyr Asn Ser Asp 
        195                 200                 205             


Pro Leu Ile Cys Arg Ala Gly Leu Lys Val Cys Phe Gly Ile Gln Leu 
    210                 215                 220                 


Leu Asn Ala Val Ser Arg Val Glu Arg Ala Leu Pro Lys Leu Thr Val 
225                 230                 235                 240 


Pro Phe Leu Leu Leu Gln Gly Ser Ala Asp Arg Leu Cys Asp Ser Lys 
                245                 250                 255     


Gly Ala Tyr Leu Leu Met Glu Leu Ala Lys Ser Gln Asp Lys Thr Leu 
            260                 265                 270         


Lys Ile Tyr Glu Gly Ala Tyr His Val Leu His Lys Glu Leu Pro Glu 
        275                 280                 285             


Val Thr Asn Ser Val Phe His Glu Ile Asn Met Trp Val Ser Gln Arg 
    290                 295                 300                 


Thr Ala Thr Ala Gly Thr Ala Ser Pro Pro 
305                 310                 


<210>  3
<211>  319
<212>  PRT
<213>  Human

<400>  3

Met Val Asp His His His His His His Glu Asn Leu Tyr Phe Gln Gly 
1               5                   10                  15      


Met Pro Glu Glu Ser Ser Pro Arg Arg Thr Pro Gln Ser Ile Pro Tyr 
            20                  25                  30          


Gln Asp Leu Pro His Leu Val Asn Ala Asp Gly Gln Tyr Leu Phe Cys 
        35                  40                  45              


Arg Tyr Trp Lys Pro Thr Gly Thr Pro Lys Ala Leu Ile Phe Val Ser 
    50                  55                  60                  


His Gly Ala Gly Glu His Ser Gly Arg Tyr Glu Glu Leu Ala Arg Met 
65                  70                  75                  80  


Leu Met Gly Leu Asp Leu Leu Val Phe Ala His Asp His Val Gly His 
                85                  90                  95      


Gly Gln Ser Glu Gly Glu Arg Met Val Val Ser Asp Phe His Val Phe 
            100                 105                 110         


Val Arg Asp Val Leu Gln His Val Asp Ser Met Gln Lys Asp Tyr Pro 
        115                 120                 125             


Gly Leu Pro Val Phe Leu Leu Gly His Ser Met Gly Gly Ala Ile Ala 
    130                 135                 140                 


Ile Leu Thr Ala Ala Glu Arg Pro Gly His Phe Ala Gly Met Val Leu 
145                 150                 155                 160 


Ile Ser Pro Leu Val Leu Ala Asn Pro Glu Ser Ala Thr Thr Phe Lys 
                165                 170                 175     


Val Leu Ala Ala Lys Val Leu Asn Leu Val Leu Pro Asn Leu Ser Leu 
            180                 185                 190         


Gly Pro Ile Asp Ser Ser Val Leu Ser Arg Asn Lys Thr Glu Val Asp 
        195                 200                 205             


Ile Tyr Asn Ser Asp Pro Leu Ile Cys Arg Ala Gly Leu Lys Val Cys 
    210                 215                 220                 


Phe Gly Ile Gln Leu Leu Asn Ala Val Ser Arg Val Glu Arg Ala Leu 
225                 230                 235                 240 


Pro Lys Leu Thr Val Pro Phe Leu Leu Leu Gln Gly Ser Ala Asp Arg 
                245                 250                 255     


Leu Cys Asp Ser Lys Gly Ala Tyr Leu Leu Met Glu Leu Ala Lys Ser 
            260                 265                 270         


Gln Asp Lys Thr Leu Lys Ile Tyr Glu Gly Ala Tyr His Val Leu His 
        275                 280                 285             


Lys Glu Leu Pro Glu Val Thr Asn Ser Val Phe His Glu Ile Asn Met 
    290                 295                 300                 


Trp Val Ser Gln Arg Thr Ala Thr Ala Gly Thr Ala Ser Pro Pro 
305                 310                 315                 


<210>  4
<211>  319
<212>  PRT
<213>  Human

<400>  4

Met Val Asp His His His His His His Glu Asn Leu Tyr Phe Gln Gly 
1               5                   10                  15      


Met Pro Glu Glu Ser Ser Pro Arg Arg Thr Pro Gln Ser Ile Pro Tyr 
            20                  25                  30          


Gln Asp Leu Pro His Leu Val Asn Ala Asp Gly Gln Tyr Leu Phe Cys 
        35                  40                  45              


Arg Tyr Trp Lys Pro Thr Gly Thr Pro Lys Ala Leu Ile Phe Val Ser 
    50                  55                  60                  


His Gly Ala Gly Glu His Ser Gly Arg Tyr Glu Glu Leu Ala Arg Met 
65                  70                  75                  80  


Leu Met Gly Leu Asp Leu Leu Val Phe Ala His Asp His Val Gly His 
                85                  90                  95      


Gly Gln Ser Glu Gly Glu Arg Met Val Val Ser Asp Phe His Val Phe 
            100                 105                 110         


Val Arg Asp Val Leu Gln His Val Asp Ser Met Gln Lys Asp Tyr Pro 
        115                 120                 125             


Gly Leu Pro Val Phe Leu Leu Gly His Ser Met Gly Gly Ala Ile Ala 
    130                 135                 140                 


Ile Leu Thr Ala Ala Glu Arg Pro Gly His Phe Ala Gly Met Val Leu 
145                 150                 155                 160 


Ile Ser Pro Leu Val Leu Ala Asn Pro Glu Ser Ala Thr Thr Phe Lys 
                165                 170                 175     


Val Leu Ala Ala Lys Val Leu Asn Ser Val Leu Pro Asn Leu Ser Ser 
            180                 185                 190         


Gly Pro Ile Asp Ser Ser Val Leu Ser Arg Asn Lys Thr Glu Val Asp 
        195                 200                 205             


Ile Tyr Asn Ser Asp Pro Leu Ile Cys Arg Ala Gly Leu Lys Val Cys 
    210                 215                 220                 


Phe Gly Ile Gln Leu Leu Asn Ala Val Ser Arg Val Glu Arg Ala Leu 
225                 230                 235                 240 


Pro Lys Leu Thr Val Pro Phe Leu Leu Leu Gln Gly Ser Ala Asp Arg 
                245                 250                 255     


Leu Cys Asp Ser Lys Gly Ala Tyr Leu Leu Met Glu Leu Ala Lys Ser 
            260                 265                 270         


Gln Asp Lys Thr Leu Lys Ile Tyr Glu Gly Ala Tyr His Val Leu His 
        275                 280                 285             


Lys Glu Leu Pro Glu Val Thr Asn Ser Val Phe His Glu Ile Asn Met 
    290                 295                 300                 


Trp Val Ser Gln Arg Thr Ala Thr Ala Gly Thr Ala Ser Pro Pro 
305                 310                 315                 


<210>  5
<211>  304
<212>  PRT
<213>  Human

<400>  5

Gly Met Pro Glu Glu Ser Ser Pro Arg Arg Thr Pro Gln Ser Ile Pro 
1               5                   10                  15      


Tyr Gln Asp Leu Pro His Leu Val Asn Ala Asp Gly Gln Tyr Leu Phe 
            20                  25                  30          


Cys Arg Tyr Trp Lys Pro Thr Gly Thr Pro Lys Ala Leu Ile Phe Val 
        35                  40                  45              


Ser His Gly Ala Gly Glu His Ser Gly Arg Tyr Glu Glu Leu Ala Arg 
    50                  55                  60                  


Met Leu Met Gly Leu Asp Leu Leu Val Phe Ala His Asp His Val Gly 
65                  70                  75                  80  


His Gly Gln Ser Glu Gly Glu Arg Met Val Val Ser Asp Phe His Val 
                85                  90                  95      


Phe Val Arg Asp Val Leu Gln His Val Asp Ser Met Gln Lys Asp Tyr 
            100                 105                 110         


Pro Gly Leu Pro Val Phe Leu Leu Gly His Ser Met Gly Gly Ala Ile 
        115                 120                 125             


Ala Ile Leu Thr Ala Ala Glu Arg Pro Gly His Phe Ala Gly Met Val 
    130                 135                 140                 


Leu Ile Ser Pro Leu Val Leu Ala Asn Pro Glu Ser Ala Thr Thr Phe 
145                 150                 155                 160 


Lys Val Leu Ala Ala Lys Val Leu Asn Ser Val Leu Pro Asn Leu Ser 
                165                 170                 175     


Ser Gly Pro Ile Asp Ser Ser Val Leu Ser Arg Asn Lys Thr Glu Val 
            180                 185                 190         


Asp Ile Tyr Asn Ser Asp Pro Leu Ile Cys Arg Ala Gly Leu Lys Val 
        195                 200                 205             


Cys Phe Gly Ile Gln Leu Leu Asn Ala Val Ser Arg Val Glu Arg Ala 
    210                 215                 220                 


Leu Pro Lys Leu Thr Val Pro Phe Leu Leu Leu Gln Gly Ser Ala Asp 
225                 230                 235                 240 


Arg Leu Cys Asp Ser Lys Gly Ala Tyr Leu Leu Met Glu Leu Ala Lys 
                245                 250                 255     


Ser Gln Asp Lys Thr Leu Lys Ile Tyr Glu Gly Ala Tyr His Val Leu 
            260                 265                 270         


His Lys Glu Leu Pro Glu Val Thr Asn Ser Val Phe His Glu Ile Asn 
        275                 280                 285             


Met Trp Val Ser Gln Arg Thr Ala Thr Ala Gly Thr Ala Ser Pro Pro 
    290                 295                 300                 


<210>  6
<211>  319
<212>  PRT
<213>  Human

<400>  6

Met Val Asp His His His His His His Glu Asn Leu Tyr Phe Gln Gly 
1               5                   10                  15      


Met Pro Glu Glu Ser Ser Pro Arg Arg Thr Pro Gln Ser Ile Pro Tyr 
            20                  25                  30          


Gln Asp Leu Pro His Leu Val Asn Ala Asp Gly Gln Tyr Leu Phe Cys 
        35                  40                  45              


Arg Tyr Trp Ala Pro Thr Gly Thr Pro Lys Ala Leu Ile Phe Val Ser 
    50                  55                  60                  


His Gly Ala Gly Glu His Ser Gly Arg Tyr Glu Glu Leu Ala Arg Met 
65                  70                  75                  80  


Leu Met Gly Leu Asp Leu Leu Val Phe Ala His Asp His Val Gly His 
                85                  90                  95      


Gly Gln Ser Glu Gly Glu Arg Met Val Val Ser Asp Phe His Val Phe 
            100                 105                 110         


Val Arg Asp Val Leu Gln His Val Asp Ser Met Gln Lys Asp Tyr Pro 
        115                 120                 125             


Gly Leu Pro Val Phe Leu Leu Gly His Ser Met Gly Gly Ala Ile Ala 
    130                 135                 140                 


Ile Leu Thr Ala Ala Glu Arg Pro Gly His Phe Ala Gly Met Val Leu 
145                 150                 155                 160 


Ile Ser Pro Leu Val Leu Ala Asn Pro Glu Ser Ala Thr Thr Phe Lys 
                165                 170                 175     


Val Leu Ala Ala Lys Val Leu Asn Ser Val Leu Pro Asn Leu Ser Ser 
            180                 185                 190         


Gly Pro Ile Asp Ser Ser Val Leu Ser Arg Asn Lys Thr Glu Val Asp 
        195                 200                 205             


Ile Tyr Asn Ser Asp Pro Leu Ile Cys Arg Ala Gly Leu Lys Val Cys 
    210                 215                 220                 


Phe Gly Ile Gln Leu Leu Asn Ala Val Ser Arg Val Glu Arg Ala Leu 
225                 230                 235                 240 


Pro Lys Leu Thr Val Pro Phe Leu Leu Leu Gln Gly Ser Ala Asp Arg 
                245                 250                 255     


Leu Cys Asp Ser Lys Gly Ala Tyr Leu Leu Met Glu Leu Ala Lys Ser 
            260                 265                 270         


Gln Asp Lys Thr Leu Lys Ile Tyr Glu Gly Ala Tyr His Val Leu His 
        275                 280                 285             


Lys Glu Leu Pro Glu Val Thr Asn Ser Val Phe His Glu Ile Asn Met 
    290                 295                 300                 


Trp Val Ser Gln Arg Thr Ala Thr Ala Gly Thr Ala Ser Pro Pro 
305                 310                 315                 


<210>  7
<211>  304
<212>  PRT
<213>  Human

<400>  7

Gly Met Pro Glu Glu Ser Ser Pro Arg Arg Thr Pro Gln Ser Ile Pro 
1               5                   10                  15      


Tyr Gln Asp Leu Pro His Leu Val Asn Ala Asp Gly Gln Tyr Leu Phe 
            20                  25                  30          


Cys Arg Tyr Trp Ala Pro Thr Gly Thr Pro Lys Ala Leu Ile Phe Val 
        35                  40                  45              


Ser His Gly Ala Gly Glu His Ser Gly Arg Tyr Glu Glu Leu Ala Arg 
    50                  55                  60                  


Met Leu Met Gly Leu Asp Leu Leu Val Phe Ala His Asp His Val Gly 
65                  70                  75                  80  


His Gly Gln Ser Glu Gly Glu Arg Met Val Val Ser Asp Phe His Val 
                85                  90                  95      


Phe Val Arg Asp Val Leu Gln His Val Asp Ser Met Gln Lys Asp Tyr 
            100                 105                 110         


Pro Gly Leu Pro Val Phe Leu Leu Gly His Ser Met Gly Gly Ala Ile 
        115                 120                 125             


Ala Ile Leu Thr Ala Ala Glu Arg Pro Gly His Phe Ala Gly Met Val 
    130                 135                 140                 


Leu Ile Ser Pro Leu Val Leu Ala Asn Pro Glu Ser Ala Thr Thr Phe 
145                 150                 155                 160 


Lys Val Leu Ala Ala Lys Val Leu Asn Ser Val Leu Pro Asn Leu Ser 
                165                 170                 175     


Ser Gly Pro Ile Asp Ser Ser Val Leu Ser Arg Asn Lys Thr Glu Val 
            180                 185                 190         


Asp Ile Tyr Asn Ser Asp Pro Leu Ile Cys Arg Ala Gly Leu Lys Val 
        195                 200                 205             


Cys Phe Gly Ile Gln Leu Leu Asn Ala Val Ser Arg Val Glu Arg Ala 
    210                 215                 220                 


Leu Pro Lys Leu Thr Val Pro Phe Leu Leu Leu Gln Gly Ser Ala Asp 
225                 230                 235                 240 


Arg Leu Cys Asp Ser Lys Gly Ala Tyr Leu Leu Met Glu Leu Ala Lys 
                245                 250                 255     


Ser Gln Asp Lys Thr Leu Lys Ile Tyr Glu Gly Ala Tyr His Val Leu 
            260                 265                 270         


His Lys Glu Leu Pro Glu Val Thr Asn Ser Val Phe His Glu Ile Asn 
        275                 280                 285             


Met Trp Val Ser Gln Arg Thr Ala Thr Ala Gly Thr Ala Ser Pro Pro 
    290                 295                 300                 


