
                                SEQUENCE LISTING

<110> YAOHUANG KE
      GUO-LIANG YU

<120> ANTI-TNFalpha ANTIBODY
  

<130> EPIT-019

<150> 61/047,358          
<151> 2008-04-23  

<160> 12

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 120
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain

<400> 1
Gln Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu Thr
 1               5                  10                  15      
Leu Ser Leu Thr Cys Ala Val Ser Gly Phe Ser Leu Ser Arg Tyr Gly
            20                  25                  30          
Ile Asn Trp Val Arg Gln Ala Pro Gly Ser Gly Leu Glu Trp Ile Gly
        35                  40                  45              
Ala Ile Gly Glu Thr Gly Arg Ala Tyr Tyr Ala Ser Trp Ala Lys Ser
    50                  55                  60                  
Arg Ser Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Val Ser Leu Lys
65                  70                  75                  80  
Met Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Phe Cys Ala Arg
                85                  90                  95      
Gly Glu Leu Phe Asn Asn Gly Trp Gly Ala Phe Asn Ile Trp Gly Pro
            100                 105                 110         
Gly Thr Met Val Thr Val Ser Ser
        115                 120 


<210> 2
<211> 110
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain

<400> 2
Ala Tyr Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Lys Cys Gln Ala Ser Glu Ser Ile Tyr Ser Ser
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Pro Pro Lys Leu Leu Ile
        35                  40                  45              
Tyr Ser Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Phe Gly Thr Ser Asn
                85                  90                  95      
Val Glu Asn Pro Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
            100                 105                 110 


<210> 3
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain fragment

<400> 3
Arg Tyr Gly Ile Asn
 1               5  


<210> 4
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain fragment

<400> 4
Ala Ile Gly Glu Thr Gly Arg Ala Tyr Tyr Ala Ser Trp Ala Lys Ser
 1               5                  10                  15      


<210> 5
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain fragment

<400> 5
Gly Glu Leu Phe Asn Asn Gly Trp Gly Ala Phe Asn Ile
 1               5                  10              


<210> 6
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain fragment

<400> 6
Gln Ala Ser Glu Ser Ile Tyr Ser Ser Leu Ala
 1               5                  10      


<210> 7
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain fragment

<400> 7
Ser Ala Ser Thr Leu Ala Ser
 1               5          


<210> 8
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain fragment

<400> 8
Gln Gln Gly Phe Gly Thr Ser Asn Val Glu Asn Pro
 1               5                  10          


<210> 9
<211> 469
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain

<400> 9
Met Glu Thr Gly Leu Arg Trp Leu Leu Leu Val Ala Val Leu Lys Gly
 1               5                  10                  15      
Val Gln Cys Gln Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro
            20                  25                  30          
Ser Glu Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Phe Ser Leu Ser
        35                  40                  45              
Arg Tyr Gly Ile Asn Trp Val Arg Gln Ala Pro Gly Ser Gly Leu Glu
    50                  55                  60                  
Trp Ile Gly Ala Ile Gly Glu Thr Gly Arg Ala Tyr Tyr Ala Ser Trp
65                  70                  75                  80  
Ala Lys Ser Arg Ser Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Val
                85                  90                  95      
Ser Leu Lys Met Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Phe
            100                 105                 110         
Cys Ala Arg Gly Glu Leu Phe Asn Asn Gly Trp Gly Ala Phe Asn Ile
        115                 120                 125             
Trp Gly Pro Gly Thr Met Val Thr Val Ser Ser Ala Ser Thr Lys Gly
    130                 135                 140                 
Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly
145                 150                 155                 160 
Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val
                165                 170                 175     
Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe
            180                 185                 190         
Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val
        195                 200                 205             
Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val
    210                 215                 220                 
Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys
225                 230                 235                 240 
Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu
                245                 250                 255     
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
            260                 265                 270         
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
        275                 280                 285             
Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
    290                 295                 300                 
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
305                 310                 315                 320 
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                325                 330                 335     
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
            340                 345                 350         
Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        355                 360                 365             
Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln
    370                 375                 380                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
385                 390                 395                 400 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                405                 410                 415     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
            420                 425                 430         
Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
        435                 440                 445             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    450                 455                 460                 
Leu Ser Pro Gly Lys
465                 


<210> 10
<211> 239
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain

<400> 10
Met Asp Thr Arg Ala Pro Thr Gln Leu Leu Gly Leu Leu Leu Leu Trp
 1               5                  10                  15      
Leu Pro Gly Ala Arg Cys Ala Tyr Gln Met Thr Gln Ser Pro Ser Ser
            20                  25                  30          
Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Lys Cys Gln Ala Ser
        35                  40                  45              
Glu Ser Ile Tyr Ser Ser Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys
    50                  55                  60                  
Pro Pro Lys Leu Leu Ile Tyr Ser Ala Ser Thr Leu Ala Ser Gly Val
65                  70                  75                  80  
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
                85                  90                  95      
Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln
            100                 105                 110         
Gly Phe Gly Thr Ser Asn Val Glu Asn Pro Phe Gly Gly Gly Thr Lys
        115                 120                 125             
Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro
    130                 135                 140                 
Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu
145                 150                 155                 160 
Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp
                165                 170                 175     
Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp
            180                 185                 190         
Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys
        195                 200                 205             
Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln
    210                 215                 220                 
Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys
225                 230                 235                 


<210> 11
<211> 470
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain

<400> 11
Met Lys His Leu Trp Phe Phe Leu Leu Leu Val Ala Ala Pro Arg Trp
 1               5                  10                  15      
Val Leu Ser Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys
            20                  25                  30          
Pro Ser Glu Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Phe Ser Leu
        35                  40                  45              
Ser Arg Tyr Gly Ile Asn Trp Val Arg Gln Ala Pro Gly Ser Gly Leu
    50                  55                  60                  
Glu Trp Ile Gly Ala Ile Gly Glu Thr Gly Arg Ala Tyr Tyr Ala Ser
65                  70                  75                  80  
Trp Ala Lys Ser Arg Ser Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln
                85                  90                  95      
Val Ser Leu Lys Met Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr
            100                 105                 110         
Phe Cys Ala Arg Gly Glu Leu Phe Asn Asn Gly Trp Gly Ala Phe Asn
        115                 120                 125             
Ile Trp Gly Pro Gly Thr Met Val Thr Val Ser Ser Ala Ser Thr Lys
    130                 135                 140                 
Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly
145                 150                 155                 160 
Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro
                165                 170                 175     
Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr
            180                 185                 190         
Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val
        195                 200                 205             
Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn
    210                 215                 220                 
Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro
225                 230                 235                 240 
Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu
                245                 250                 255     
Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
            260                 265                 270         
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
        275                 280                 285             
Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly
    290                 295                 300                 
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
305                 310                 315                 320 
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp
                325                 330                 335     
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro
            340                 345                 350         
Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
        355                 360                 365             
Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn
    370                 375                 380                 
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile
385                 390                 395                 400 
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr
                405                 410                 415     
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys
            420                 425                 430         
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys
        435                 440                 445             
Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
    450                 455                 460                 
Ser Leu Ser Pro Gly Lys
465                 470 


<210> 12
<211> 239
<212> PRT
<213> Artificial Sequence

<220> 
<223> modified variable domain

<400> 12
Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp
 1               5                  10                  15      
Leu Arg Gly Ala Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser
            20                  25                  30          
Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Lys Cys Gln Ala Ser
        35                  40                  45              
Glu Ser Ile Tyr Ser Ser Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys
    50                  55                  60                  
Pro Pro Lys Leu Leu Ile Tyr Ser Ala Ser Thr Leu Ala Ser Gly Val
65                  70                  75                  80  
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
                85                  90                  95      
Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln
            100                 105                 110         
Gly Phe Gly Thr Ser Asn Val Glu Asn Pro Phe Gly Gly Gly Thr Lys
        115                 120                 125             
Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro
    130                 135                 140                 
Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu
145                 150                 155                 160 
Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp
                165                 170                 175     
Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp
            180                 185                 190         
Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys
        195                 200                 205             
Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln
    210                 215                 220                 
Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys
225                 230                 235                 

