                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
       Raemakers-Franken, Petronella, Catharina
       Schurmann, Martin
       Trefzer, Axel Christoph
       De Wildeman, Stefaan Marie Andre
 
<120>  Preparation of 6-amino caproic acid from alpha-ketopimelic acid

<130>  P84198PC00

<150>  EP 08152584.2
<151>  2008-03-11

<160>  83    

<170>  PatentIn version 3.3

<210>  1
<211>  1362
<212>  DNA
<213>  Vibrio fluvialis


<220>
<221>  CDS
<222>  (1)..(1362)

<400>  1
atg aac aaa ccg caa agc tgg gaa gcc cgg gcc gag acc tat tcg ctc       48
Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu         
1               5                   10                  15              

tat ggt ttc acc gac atg cct tcg ctg cat cag cgc ggc acg gtc gtc       96
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val         
            20                  25                  30                  

gtg acc cat ggc gag gga ccc tat atc gtc gat gtg aat ggc cgg cgt      144
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val Asn Gly Arg Arg         
        35                  40                  45                      

tat ctg gac gcc aac tcg ggc ctg tgg aac atg gtc gcg ggc ttt gac      192
Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp         
    50                  55                  60                          

cac aag ggg ctg atc gac gcc gcc aag gcc caa tac gag cgt ttt ccc      240
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro         
65                  70                  75                  80          

ggt tat cac gcc ttt ttc ggc cgc atg tcc gat cag acg gta atg ctg      288
Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu         
                85                  90                  95              

tcg gaa aag ctg gtc gag gtg tcg ccc ttt gat tcg ggc cgg gtg ttc      336
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe         
            100                 105                 110                 

tat aca aac tcg ggg tcc gag gcg aat gac acc atg gtc aag atg cta      384
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu         
        115                 120                 125                     

tgg ttc ctg cat gca gcc gag ggc aaa ccg caa aag cgc aag atc ctg      432
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu         
    130                 135                 140                         

acc cgc tgg aac gcc tat cac ggc gtg acc gcc gtt tcg gcc agc atg      480
Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met         
145                 150                 155                 160         

acc ggc aag ccc tat aat tcg gtc ttt ggc ctg ccg ctg ccg ggc ttt      528
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe         
                165                 170                 175             

gtg cat ctg acc tgc ccg cat tac tgg cgc tat ggc gaa gag ggc gaa      576
Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu         
            180                 185                 190                 

acc gaa gag cag ttc gtc gcc cgc ctc gcc cgc gag ctg gag gaa acg      624
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr         
        195                 200                 205                     

atc cag cgc gag ggc gcc gac acc atc gcc ggt ttc ttt gcc gaa ccg      672
Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro         
    210                 215                 220                         

gtg atg ggc gcg ggc ggc gtg att ccc ccg gcc aag ggc tat ttc cag      720
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln         
225                 230                 235                 240         

gcg atc ctg cca atc ctg cgc aaa tat gac atc ccg gtc atc tcg gac      768
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp         
                245                 250                 255             

gag gtg atc tgc ggt ttc gga cgc acc ggt aac acc tgg ggc tgc gtg      816
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val         
            260                 265                 270                 

acc tat gac ttt aca ccc gat gca atc atc tcg tcc aag aat ctt aca      864
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr         
        275                 280                 285                     

gcg ggc ttt ttc ccc atg ggg gcg gtg atc ctt ggc ccg gaa ctt tcc      912
Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu Leu Ser         
    290                 295                 300                         

aaa cgg ctg gaa acc gca atc gag gcg atc gag gaa ttc ccc cat ggc      960
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly         
305                 310                 315                 320         

ttt acc gcc tcg ggc cat ccg gtc ggc tgt gct att gcg ctg aaa gca     1008
Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala         
                325                 330                 335             

atc gac gtg gtg atg aat gaa ggg ctg gct gag aac gtc cgc cgc ctt     1056
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu         
            340                 345                 350                 

gcc ccc cgt ttc gag gaa agg ctg aaa cat atc gcc gag cgc ccg aac     1104
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn         
        355                 360                 365                     

atc ggt gaa tat cgc ggc atc ggc ttc atg tgg gcg ctg gag gct gtc     1152
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val         
    370                 375                 380                         

aag gac aag gca agc aag acg ccg ttc gac ggc aac ctg tcg gtc agc     1200
Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser         
385                 390                 395                 400         

gag cgt atc gcc aat acc tgc acc gat ctg ggg ctg att tgc cgg ccg     1248
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro         
                405                 410                 415             

ctt ggt cag tcc gtc gtc ctt tgt ccg ccc ttt atc ctg acc gag gcg     1296
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala         
            420                 425                 430                 

cag atg gat gag atg ttc gat aaa ctc gaa aaa gcc ctt gat aag gtc     1344
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val         
        435                 440                 445                     

ttt gcc gag gtt gcc tga                                             1362
Phe Ala Glu Val Ala                                                     
    450                                                                 


<210>  2
<211>  453
<212>  PRT
<213>  Vibrio fluvialis

<400>  2

Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val Asn Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 


Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  3
<211>  1362
<212>  DNA
<213>  Artificial

<220>
<223>  Vibrio fluvialis JS17 omega-aminotransferase codon optimised gene

<400>  3
atgaataaac cacagtcttg ggaagctcgt gctgaaacct atagcctgta cggctttacc     60

gatatgccgt ctctgcacca gcgtggtact gtagtggtaa cgcacggtga gggcccgtac    120

atcgtggacg ttaatggccg ccgttacctg gatgcaaaca gcggcctgtg gaacatggtt    180

gcgggcttcg accacaaagg cctgatcgat gccgcaaaag cgcagtacga acgcttcccg    240

ggttatcacg cgttctttgg ccgtatgagc gaccagactg tgatgctgag cgaaaaactg    300

gttgaagtgt ccccgttcga tagcggtcgt gtcttttaca ctaactctgg cagcgaggct    360

aacgatacca tggttaagat gctgtggttc ctgcacgcag cggaaggcaa acctcagaaa    420

cgtaaaattc tgacccgttg gaacgcttat cacggtgtga ctgctgtttc cgcatctatg    480

accggtaaac cgtataacag cgtgttcggt ctgccgctgc ctggcttcgt gcatctgacc    540

tgcccgcact actggcgtta tggtgaggaa ggcgaaactg aggaacagtt cgtggcgcgt    600

ctggctcgtg aactggaaga aaccattcaa cgcgaaggtg cagatactat cgcgggcttc    660

tttgcggagc ctgttatggg tgccggcggt gtgattccgc cggcgaaggg ctatttccag    720

gcaatcctgc cgatcctgcg caagtacgac attccggtta tttctgacga agtgatctgc    780

ggcttcggcc gcaccggtaa cacctggggc tgcgtgacgt atgacttcac tccggacgca    840

atcattagct ctaaaaacct gactgcgggt ttcttcccta tgggcgccgt aatcctgggc    900

ccagaactgt ctaagcgcct ggaaaccgcc atcgaggcaa tcgaagagtt cccgcacggt    960

ttcactgcta gcggccatcc ggtaggctgc gcaatcgcgc tgaaggcgat cgatgttgtc   1020

atgaacgagg gcctggcgga aaacgtgcgc cgcctggcgc cgcgttttga agaacgtctg   1080

aaacacattg ctgagcgccc gaacattggc gaatatcgcg gcatcggttt catgtgggcc   1140

ctggaagcag ttaaagataa agctagcaag accccgttcg acggcaacct gtccgtgagc   1200

gaacgtatcg ctaatacctg tacggacctg ggtctgatct gccgtccgct gggtcagtcc   1260

gtagttctgt gcccaccatt tatcctgacc gaagcgcaga tggatgaaat gttcgataaa   1320

ctggagaaag ctctggataa agtgttcgct gaagtcgcgt aa                      1362


<210>  4
<211>  1350
<212>  DNA
<213>  Bacillus weihenstephanensis


<220>
<221>  CDS
<222>  (1)..(1350)

<400>  4
gtg caa gcg acg gag caa aca caa agt ttg aaa aaa aca gat gaa aag       48
Val Gln Ala Thr Glu Gln Thr Gln Ser Leu Lys Lys Thr Asp Glu Lys         
1               5                   10                  15              

tac ctt tgg cat gcg atg aga gga gca gcc cct agt cca acg aat tta       96
Tyr Leu Trp His Ala Met Arg Gly Ala Ala Pro Ser Pro Thr Asn Leu         
            20                  25                  30                  

att atc aca aaa gca gaa ggg gca tgg gtg acg gat att gat gga aac      144
Ile Ile Thr Lys Ala Glu Gly Ala Trp Val Thr Asp Ile Asp Gly Asn         
        35                  40                  45                      

cgt tat tta gac ggt atg tcc ggt ctt tgg tgc gtg aat gtt ggg tat      192
Arg Tyr Leu Asp Gly Met Ser Gly Leu Trp Cys Val Asn Val Gly Tyr         
    50                  55                  60                          

ggt cga aaa gaa ctt gca aga gcg gcg ttt gaa cag ctt gaa gaa atg      240
Gly Arg Lys Glu Leu Ala Arg Ala Ala Phe Glu Gln Leu Glu Glu Met         
65                  70                  75                  80          

ccg tat ttc cct ctg act caa agt cat gtt cct gct att aaa tta gca      288
Pro Tyr Phe Pro Leu Thr Gln Ser His Val Pro Ala Ile Lys Leu Ala         
                85                  90                  95              

gaa aaa ttg aat gaa tgg ctt gat gat gaa tac gtc att ttc ttt tct      336
Glu Lys Leu Asn Glu Trp Leu Asp Asp Glu Tyr Val Ile Phe Phe Ser         
            100                 105                 110                 

aac agt gga tcg gaa gcg aat gaa aca gca ttt aaa att gct cgt caa      384
Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys Ile Ala Arg Gln         
        115                 120                 125                     

tat cat caa caa aaa ggt gat cat gga cgc tat aag ttt att tcc cgc      432
Tyr His Gln Gln Lys Gly Asp His Gly Arg Tyr Lys Phe Ile Ser Arg         
    130                 135                 140                         

tac cgc gct tat cac ggt aac tca atg gga gct ctt gca gca aca ggt      480
Tyr Arg Ala Tyr His Gly Asn Ser Met Gly Ala Leu Ala Ala Thr Gly         
145                 150                 155                 160         

caa gca cag cga aag tat aaa tat gaa cca ctc ggg caa gga ttc ctg      528
Gln Ala Gln Arg Lys Tyr Lys Tyr Glu Pro Leu Gly Gln Gly Phe Leu         
                165                 170                 175             

cat gta gca ccg cct gat acg tat cga aat cca gag gat gtt cat aca      576
His Val Ala Pro Pro Asp Thr Tyr Arg Asn Pro Glu Asp Val His Thr         
            180                 185                 190                 

ctg gca agt gct gag gaa atc gat cgt gtc atg aca tgg gag tta agc      624
Leu Ala Ser Ala Glu Glu Ile Asp Arg Val Met Thr Trp Glu Leu Ser         
        195                 200                 205                     

caa aca gta gcc ggt gtg att atg gag cca atc att act ggg ggc gga      672
Gln Thr Val Ala Gly Val Ile Met Glu Pro Ile Ile Thr Gly Gly Gly         
    210                 215                 220                         

att tta atg cct cct gat gga tat atg gga aaa gta aaa gaa att tgc      720
Ile Leu Met Pro Pro Asp Gly Tyr Met Gly Lys Val Lys Glu Ile Cys         
225                 230                 235                 240         

gag aag cac ggt gcg ttg ctc att tgt gat gaa gtt ata tgt gga ttt      768
Glu Lys His Gly Ala Leu Leu Ile Cys Asp Glu Val Ile Cys Gly Phe         
                245                 250                 255             

ggc cgg aca ggg aag cca ttt gga ttt atg aat tat ggc gtc aaa cca      816
Gly Arg Thr Gly Lys Pro Phe Gly Phe Met Asn Tyr Gly Val Lys Pro         
            260                 265                 270                 

gat atc att aca atg gca aaa ggt att aca agt gcg tat ctt cct ttg      864
Asp Ile Ile Thr Met Ala Lys Gly Ile Thr Ser Ala Tyr Leu Pro Leu         
        275                 280                 285                     

tca gca aca gca gtt aga cga gag gtt tat gag gca ttc gta ggt agt      912
Ser Ala Thr Ala Val Arg Arg Glu Val Tyr Glu Ala Phe Val Gly Ser         
    290                 295                 300                         

gat gat tat gat cgc ttc cgc cat gta aat acg ttc gga ggg aat cct      960
Asp Asp Tyr Asp Arg Phe Arg His Val Asn Thr Phe Gly Gly Asn Pro         
305                 310                 315                 320         

gct gct tgc gct tta gct ttg aag aat tta gaa att atg gag aat gag     1008
Ala Ala Cys Ala Leu Ala Leu Lys Asn Leu Glu Ile Met Glu Asn Glu         
                325                 330                 335             

aaa ctc att gaa cgt tcc aaa gaa ttg ggt gaa cga ctg tta tat gag     1056
Lys Leu Ile Glu Arg Ser Lys Glu Leu Gly Glu Arg Leu Leu Tyr Glu         
            340                 345                 350                 

cta gag gat gta aaa gag cat cca aac gta ggg gat gtt cgc gga aag     1104
Leu Glu Asp Val Lys Glu His Pro Asn Val Gly Asp Val Arg Gly Lys         
        355                 360                 365                     

ggc ctt ctt tta ggc att gaa cta gtg gaa gat aag caa aca aaa gaa     1152
Gly Leu Leu Leu Gly Ile Glu Leu Val Glu Asp Lys Gln Thr Lys Glu         
    370                 375                 380                         

ccg gct tcc att gaa aag atg aac aaa gtc atc aat gct tgt aaa gaa     1200
Pro Ala Ser Ile Glu Lys Met Asn Lys Val Ile Asn Ala Cys Lys Glu         
385                 390                 395                 400         

aaa ggt cta att att ggt aaa aat ggt gac act gtc gca ggt tac aat     1248
Lys Gly Leu Ile Ile Gly Lys Asn Gly Asp Thr Val Ala Gly Tyr Asn         
                405                 410                 415             

aat att ttg cag ctt gca cct cca tta agc atc aca gag gaa gac ttt     1296
Asn Ile Leu Gln Leu Ala Pro Pro Leu Ser Ile Thr Glu Glu Asp Phe         
            420                 425                 430                 

act ttt atc gtt aaa aca atg aaa gaa tgt tta tcc cgc att aac ggg     1344
Thr Phe Ile Val Lys Thr Met Lys Glu Cys Leu Ser Arg Ile Asn Gly         
        435                 440                 445                     

cag taa                                                             1350
Gln                                                                     
                                                                        


<210>  5
<211>  449
<212>  PRT
<213>  Bacillus weihenstephanensis

<400>  5

Val Gln Ala Thr Glu Gln Thr Gln Ser Leu Lys Lys Thr Asp Glu Lys 
1               5                   10                  15      


Tyr Leu Trp His Ala Met Arg Gly Ala Ala Pro Ser Pro Thr Asn Leu 
            20                  25                  30          


Ile Ile Thr Lys Ala Glu Gly Ala Trp Val Thr Asp Ile Asp Gly Asn 
        35                  40                  45              


Arg Tyr Leu Asp Gly Met Ser Gly Leu Trp Cys Val Asn Val Gly Tyr 
    50                  55                  60                  


Gly Arg Lys Glu Leu Ala Arg Ala Ala Phe Glu Gln Leu Glu Glu Met 
65                  70                  75                  80  


Pro Tyr Phe Pro Leu Thr Gln Ser His Val Pro Ala Ile Lys Leu Ala 
                85                  90                  95      


Glu Lys Leu Asn Glu Trp Leu Asp Asp Glu Tyr Val Ile Phe Phe Ser 
            100                 105                 110         


Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys Ile Ala Arg Gln 
        115                 120                 125             


Tyr His Gln Gln Lys Gly Asp His Gly Arg Tyr Lys Phe Ile Ser Arg 
    130                 135                 140                 


Tyr Arg Ala Tyr His Gly Asn Ser Met Gly Ala Leu Ala Ala Thr Gly 
145                 150                 155                 160 


Gln Ala Gln Arg Lys Tyr Lys Tyr Glu Pro Leu Gly Gln Gly Phe Leu 
                165                 170                 175     


His Val Ala Pro Pro Asp Thr Tyr Arg Asn Pro Glu Asp Val His Thr 
            180                 185                 190         


Leu Ala Ser Ala Glu Glu Ile Asp Arg Val Met Thr Trp Glu Leu Ser 
        195                 200                 205             


Gln Thr Val Ala Gly Val Ile Met Glu Pro Ile Ile Thr Gly Gly Gly 
    210                 215                 220                 


Ile Leu Met Pro Pro Asp Gly Tyr Met Gly Lys Val Lys Glu Ile Cys 
225                 230                 235                 240 


Glu Lys His Gly Ala Leu Leu Ile Cys Asp Glu Val Ile Cys Gly Phe 
                245                 250                 255     


Gly Arg Thr Gly Lys Pro Phe Gly Phe Met Asn Tyr Gly Val Lys Pro 
            260                 265                 270         


Asp Ile Ile Thr Met Ala Lys Gly Ile Thr Ser Ala Tyr Leu Pro Leu 
        275                 280                 285             


Ser Ala Thr Ala Val Arg Arg Glu Val Tyr Glu Ala Phe Val Gly Ser 
    290                 295                 300                 


Asp Asp Tyr Asp Arg Phe Arg His Val Asn Thr Phe Gly Gly Asn Pro 
305                 310                 315                 320 


Ala Ala Cys Ala Leu Ala Leu Lys Asn Leu Glu Ile Met Glu Asn Glu 
                325                 330                 335     


Lys Leu Ile Glu Arg Ser Lys Glu Leu Gly Glu Arg Leu Leu Tyr Glu 
            340                 345                 350         


Leu Glu Asp Val Lys Glu His Pro Asn Val Gly Asp Val Arg Gly Lys 
        355                 360                 365             


Gly Leu Leu Leu Gly Ile Glu Leu Val Glu Asp Lys Gln Thr Lys Glu 
    370                 375                 380                 


Pro Ala Ser Ile Glu Lys Met Asn Lys Val Ile Asn Ala Cys Lys Glu 
385                 390                 395                 400 


Lys Gly Leu Ile Ile Gly Lys Asn Gly Asp Thr Val Ala Gly Tyr Asn 
                405                 410                 415     


Asn Ile Leu Gln Leu Ala Pro Pro Leu Ser Ile Thr Glu Glu Asp Phe 
            420                 425                 430         


Thr Phe Ile Val Lys Thr Met Lys Glu Cys Leu Ser Arg Ile Asn Gly 
        435                 440                 445             


Gln 
    


<210>  6
<211>  1350
<212>  DNA
<213>  Artificial

<220>
<223>  B. weihenstephanensis KBAB4 aminotransferase codon-optimised gene

<400>  6
atgcaggcta ccgaacaaac ccaatctctg aaaaagactg acgaaaaata tctgtggcac     60

gcgatgcgcg gtgcagctcc gtctccgacc aacctgatta ttaccaaagc tgaaggcgcg    120

tgggtgaccg acattgacgg taaccgttat ctggatggca tgagcggcct gtggtgtgtt    180

aatgtcggtt atggccgtaa ggagctggcg cgcgcggcat ttgaacaact ggaagaaatg    240

ccgtacttcc cgctgactca aagccatgtg ccggctatca aactggcgga aaaactgaac    300

gaatggctgg acgacgaata cgtgattttc ttctctaatt ctggctccga agcaaacgaa    360

accgcattca aaatcgcccg tcaatatcac cagcagaaag gtgaccacgg ccgctataaa    420

ttcatcagcc gttatcgtgc ataccatggt aattctatgg gtgcgctggc tgctaccggt    480

caggctcagc gcaaatacaa gtacgaaccg ctgggtcagg gttttctgca cgttgcacca    540

ccggatacct accgtaaccc ggaagacgtc cacaccctgg cttctgccga agaaatcgat    600

cgtgttatga cctgggagct gtcccagact gttgcgggtg ttatcatgga acctattatt    660

accggtggtg gcattctgat gccgccggac ggttatatgg gtaaagtcaa ggaaatctgc    720

gaaaaacacg gcgcgctgct gatctgcgat gaagttatct gtggcttcgg tcgcaccggc    780

aaaccatttg gcttcatgaa ttatggcgta aaacctgaca ttattaccat ggctaaaggc    840

attacttccg cttatctgcc gctgagcgcg accgcagttc gccgcgaagt ttatgaagcg    900

tttgttggtt ctgatgatta cgaccgtttc cgtcatgtaa acacgtttgg cggtaaccca    960

gcggcatgtg cgctggcgct gaaaaacctg gaaatcatgg aaaacgaaaa gctgatcgaa   1020

cgtagcaaag aactgggtga acgtctgctg tacgaactgg aagatgtcaa agaacacccg   1080

aacgtgggcg atgttcgcgg taaaggcctg ctgctgggta ttgaactggt tgaagacaaa   1140

cagaccaagg aaccggcttc cattgaaaag atgaacaaag tgattaacgc gtgcaaagag   1200

aaaggcctga tcattggtaa gaacggtgat accgtggcag gttataacaa cattctgcag   1260

ctggcgccgc ctctgagcat cactgaagaa gatttcacct tcatcgtcaa aactatgaag   1320

gagtgcctga gccgcatcaa tggtcagtaa                                    1350


<210>  7
<211>  1371
<212>  DNA
<213>  Pseudomonas aeruginosa (gi 9946143)


<220>
<221>  CDS
<222>  (1)..(1371)

<400>  7
atg aac agc caa atc acc aac gcc aag acc cgt gag tgg cag gcg ttg       48
Met Asn Ser Gln Ile Thr Asn Ala Lys Thr Arg Glu Trp Gln Ala Leu         
1               5                   10                  15              

agc cgc gac cac cat ctg ccg ccg ttc acc gac tac aag cag ttg aac       96
Ser Arg Asp His His Leu Pro Pro Phe Thr Asp Tyr Lys Gln Leu Asn         
            20                  25                  30                  

gag aag ggc gcg cgg atc atc acc aag gcc gaa ggc gtc tat atc tgg      144
Glu Lys Gly Ala Arg Ile Ile Thr Lys Ala Glu Gly Val Tyr Ile Trp         
        35                  40                  45                      

gac agc gag ggc aac aag atc ctc gat gcg atg gcc ggc ctc tgg tgc      192
Asp Ser Glu Gly Asn Lys Ile Leu Asp Ala Met Ala Gly Leu Trp Cys         
    50                  55                  60                          

gtc aac gtc ggc tac ggc cgc gag gag ctg gtc cag gcc gcc acc cgg      240
Val Asn Val Gly Tyr Gly Arg Glu Glu Leu Val Gln Ala Ala Thr Arg         
65                  70                  75                  80          

cag atg cgc gag ttg ccg ttc tac aac ctg ttc ttc cag acc gcc cac      288
Gln Met Arg Glu Leu Pro Phe Tyr Asn Leu Phe Phe Gln Thr Ala His         
                85                  90                  95              

ccg ccg gtg gtc gag ctg gcc aag gcg atc gcc gac gtc gct ccg gaa      336
Pro Pro Val Val Glu Leu Ala Lys Ala Ile Ala Asp Val Ala Pro Glu         
            100                 105                 110                 

ggc atg aac cac gtg ttc ttc acc ggc tcc ggc tcc gag gcc aac gac      384
Gly Met Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Ala Asn Asp         
        115                 120                 125                     

acc gtg ctg cgt atg gtc cgc cac tat tgg gcg acc aag ggc cag ccg      432
Thr Val Leu Arg Met Val Arg His Tyr Trp Ala Thr Lys Gly Gln Pro         
    130                 135                 140                         

cag aag aaa gtg gtg atc ggc cgc tgg aac ggc tac cac ggc tcc acc      480
Gln Lys Lys Val Val Ile Gly Arg Trp Asn Gly Tyr His Gly Ser Thr         
145                 150                 155                 160         

gtc gcc ggc gtc agc ctg ggc ggc atg aag gcg ttg cat gag cag ggt      528
Val Ala Gly Val Ser Leu Gly Gly Met Lys Ala Leu His Glu Gln Gly         
                165                 170                 175             

gat ttc ccc atc ccg ggc atc gtc cac atc gcc cag ccc tac tgg tac      576
Asp Phe Pro Ile Pro Gly Ile Val His Ile Ala Gln Pro Tyr Trp Tyr         
            180                 185                 190                 

ggc gag ggc ggc gac atg tcg ccg gac gag ttc ggc gtc tgg gcc gcc      624
Gly Glu Gly Gly Asp Met Ser Pro Asp Glu Phe Gly Val Trp Ala Ala         
        195                 200                 205                     

gag cag ttg gag aag aag att ctc gaa gtg ggc gag gaa aac gtc gcc      672
Glu Gln Leu Glu Lys Lys Ile Leu Glu Val Gly Glu Glu Asn Val Ala         
    210                 215                 220                         

gcc ttc atc gcc gag ccg atc cag ggc gcc ggc ggc gtg atc gtc ccg      720
Ala Phe Ile Ala Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro         
225                 230                 235                 240         

ccg gac acc tac tgg ccg aag atc cgc gag atc ctc gcc aag tac gac      768
Pro Asp Thr Tyr Trp Pro Lys Ile Arg Glu Ile Leu Ala Lys Tyr Asp         
                245                 250                 255             

atc ctg ttc atc gcc gac gaa gtg atc tgc ggc ttc ggc cgt acc ggc      816
Ile Leu Phe Ile Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly         
            260                 265                 270                 

gag tgg ttc ggc agc cag tac tac ggc aac gcc ccg gac ctg atg ccg      864
Glu Trp Phe Gly Ser Gln Tyr Tyr Gly Asn Ala Pro Asp Leu Met Pro         
        275                 280                 285                     

atc gcc aag ggc ctc acc tcc ggc tac atc ccc atg ggc ggg gtg gtg      912
Ile Ala Lys Gly Leu Thr Ser Gly Tyr Ile Pro Met Gly Gly Val Val         
    290                 295                 300                         

gtg cgc gac gag atc gtc gaa gtg ctc aac cag ggc ggc gag ttc tac      960
Val Arg Asp Glu Ile Val Glu Val Leu Asn Gln Gly Gly Glu Phe Tyr         
305                 310                 315                 320         

cac ggc ttc acc tat tcc ggt cac ccg gtg gcg gcc gcc gtg gcc ctg     1008
His Gly Phe Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Ala Leu         
                325                 330                 335             

gag aac atc cgc atc ctg cgc gaa gag aag atc atc gag aag gtg aag     1056
Glu Asn Ile Arg Ile Leu Arg Glu Glu Lys Ile Ile Glu Lys Val Lys         
            340                 345                 350                 

gcg gaa acg gca ccg tat ttg cag aaa cgc tgg cag gag ctg gcc gac     1104
Ala Glu Thr Ala Pro Tyr Leu Gln Lys Arg Trp Gln Glu Leu Ala Asp         
        355                 360                 365                     

cac ccg ttg gtg ggc gaa gcg cgc ggg gtc ggc atg gtc gcc gcc ctg     1152
His Pro Leu Val Gly Glu Ala Arg Gly Val Gly Met Val Ala Ala Leu         
    370                 375                 380                         

gag ctg gtc aag aac aag aag acc cgc gag cgt ttc acc gac aag ggc     1200
Glu Leu Val Lys Asn Lys Lys Thr Arg Glu Arg Phe Thr Asp Lys Gly         
385                 390                 395                 400         

gtc ggg atg ctg tgc cgg gaa cat tgt ttc cgc aac ggt ttg atc atg     1248
Val Gly Met Leu Cys Arg Glu His Cys Phe Arg Asn Gly Leu Ile Met         
                405                 410                 415             

cgc gcg gtg ggc gac act atg att atc tcg ccg ccg ctg gtg atc gat     1296
Arg Ala Val Gly Asp Thr Met Ile Ile Ser Pro Pro Leu Val Ile Asp         
            420                 425                 430                 

ccg tcg cag atc gat gag ttg atc acc ctg gcg cgc aag tgc ctc gat     1344
Pro Ser Gln Ile Asp Glu Leu Ile Thr Leu Ala Arg Lys Cys Leu Asp         
        435                 440                 445                     

cag acc gcc gcc gcc gtc ctg gct tga                                 1371
Gln Thr Ala Ala Ala Val Leu Ala                                         
    450                 455                                             


<210>  8
<211>  456
<212>  PRT
<213>  Pseudomonas aeruginosa (gi 9946143)

<400>  8

Met Asn Ser Gln Ile Thr Asn Ala Lys Thr Arg Glu Trp Gln Ala Leu 
1               5                   10                  15      


Ser Arg Asp His His Leu Pro Pro Phe Thr Asp Tyr Lys Gln Leu Asn 
            20                  25                  30          


Glu Lys Gly Ala Arg Ile Ile Thr Lys Ala Glu Gly Val Tyr Ile Trp 
        35                  40                  45              


Asp Ser Glu Gly Asn Lys Ile Leu Asp Ala Met Ala Gly Leu Trp Cys 
    50                  55                  60                  


Val Asn Val Gly Tyr Gly Arg Glu Glu Leu Val Gln Ala Ala Thr Arg 
65                  70                  75                  80  


Gln Met Arg Glu Leu Pro Phe Tyr Asn Leu Phe Phe Gln Thr Ala His 
                85                  90                  95      


Pro Pro Val Val Glu Leu Ala Lys Ala Ile Ala Asp Val Ala Pro Glu 
            100                 105                 110         


Gly Met Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Ala Asn Asp 
        115                 120                 125             


Thr Val Leu Arg Met Val Arg His Tyr Trp Ala Thr Lys Gly Gln Pro 
    130                 135                 140                 


Gln Lys Lys Val Val Ile Gly Arg Trp Asn Gly Tyr His Gly Ser Thr 
145                 150                 155                 160 


Val Ala Gly Val Ser Leu Gly Gly Met Lys Ala Leu His Glu Gln Gly 
                165                 170                 175     


Asp Phe Pro Ile Pro Gly Ile Val His Ile Ala Gln Pro Tyr Trp Tyr 
            180                 185                 190         


Gly Glu Gly Gly Asp Met Ser Pro Asp Glu Phe Gly Val Trp Ala Ala 
        195                 200                 205             


Glu Gln Leu Glu Lys Lys Ile Leu Glu Val Gly Glu Glu Asn Val Ala 
    210                 215                 220                 


Ala Phe Ile Ala Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro 
225                 230                 235                 240 


Pro Asp Thr Tyr Trp Pro Lys Ile Arg Glu Ile Leu Ala Lys Tyr Asp 
                245                 250                 255     


Ile Leu Phe Ile Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly 
            260                 265                 270         


Glu Trp Phe Gly Ser Gln Tyr Tyr Gly Asn Ala Pro Asp Leu Met Pro 
        275                 280                 285             


Ile Ala Lys Gly Leu Thr Ser Gly Tyr Ile Pro Met Gly Gly Val Val 
    290                 295                 300                 


Val Arg Asp Glu Ile Val Glu Val Leu Asn Gln Gly Gly Glu Phe Tyr 
305                 310                 315                 320 


His Gly Phe Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Ala Leu 
                325                 330                 335     


Glu Asn Ile Arg Ile Leu Arg Glu Glu Lys Ile Ile Glu Lys Val Lys 
            340                 345                 350         


Ala Glu Thr Ala Pro Tyr Leu Gln Lys Arg Trp Gln Glu Leu Ala Asp 
        355                 360                 365             


His Pro Leu Val Gly Glu Ala Arg Gly Val Gly Met Val Ala Ala Leu 
    370                 375                 380                 


Glu Leu Val Lys Asn Lys Lys Thr Arg Glu Arg Phe Thr Asp Lys Gly 
385                 390                 395                 400 


Val Gly Met Leu Cys Arg Glu His Cys Phe Arg Asn Gly Leu Ile Met 
                405                 410                 415     


Arg Ala Val Gly Asp Thr Met Ile Ile Ser Pro Pro Leu Val Ile Asp 
            420                 425                 430         


Pro Ser Gln Ile Asp Glu Leu Ile Thr Leu Ala Arg Lys Cys Leu Asp 
        435                 440                 445             


Gln Thr Ala Ala Ala Val Leu Ala 
    450                 455     


<210>  9
<211>  70
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  9
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgaaca gccaaatcac     60

caacgccaag                                                            70


<210>  10
<211>  49
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  10
ggggaccact ttgtacaaga aagctgggtt caagccagga cggcggcgg                 49


<210>  11
<211>  1365
<212>  DNA
<213>  Pseudomonas syringae


<220>
<221>  CDS
<222>  (1)..(1365)

<400>  11
atg agt gcc aac aac ccg caa acc ctc gaa tgg cag gcc ctg agc agc       48
Met Ser Ala Asn Asn Pro Gln Thr Leu Glu Trp Gln Ala Leu Ser Ser         
1               5                   10                  15              

gag cat cac ctg gca ccg ttc agc gac tac aaa caa ctg aaa gag aaa       96
Glu His His Leu Ala Pro Phe Ser Asp Tyr Lys Gln Leu Lys Glu Lys         
            20                  25                  30                  

ggc ccg cgc atc atc acc cgt gcc gag ggc gtt tat ctg tgg gac agc      144
Gly Pro Arg Ile Ile Thr Arg Ala Glu Gly Val Tyr Leu Trp Asp Ser         
        35                  40                  45                      

gag ggc aac aag atc ctc gat ggc atg tcc ggc ctg tgg tgc gtg gcc      192
Glu Gly Asn Lys Ile Leu Asp Gly Met Ser Gly Leu Trp Cys Val Ala         
    50                  55                  60                          

atc ggt tat ggc cgc gaa gaa ctg gcc gac gca gcc agc aaa cag atg      240
Ile Gly Tyr Gly Arg Glu Glu Leu Ala Asp Ala Ala Ser Lys Gln Met         
65                  70                  75                  80          

cgc gag ctg ccg tac tac aac ctg ttc ttc cag acc gcc cac ccg ccg      288
Arg Glu Leu Pro Tyr Tyr Asn Leu Phe Phe Gln Thr Ala His Pro Pro         
                85                  90                  95              

gtg ctg gaa ctg gcc aag gcc atc tcc gac atc gct ccc gag ggc atg      336
Val Leu Glu Leu Ala Lys Ala Ile Ser Asp Ile Ala Pro Glu Gly Met         
            100                 105                 110                 

aac cat gtg ttc ttc acc ggt tca ggc tct gaa ggc aat gac acg atg      384
Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Gly Asn Asp Thr Met         
        115                 120                 125                     

ctg cgc atg gtt cgt cat tac tgg gcg ctg aaa ggc cag ccg aac aag      432
Leu Arg Met Val Arg His Tyr Trp Ala Leu Lys Gly Gln Pro Asn Lys         
    130                 135                 140                         

aaa acc atc atc agc cgc gtc aat ggc tac cac ggc tcc acc gtc gcc      480
Lys Thr Ile Ile Ser Arg Val Asn Gly Tyr His Gly Ser Thr Val Ala         
145                 150                 155                 160         

ggt gcc agc ctg ggt ggc atg acc tac atg cac gaa cag ggc gac ctg      528
Gly Ala Ser Leu Gly Gly Met Thr Tyr Met His Glu Gln Gly Asp Leu         
                165                 170                 175             

ccg atc ccg ggg gtg gtg cac att cca cag cct tac tgg ttc ggc gaa      576
Pro Ile Pro Gly Val Val His Ile Pro Gln Pro Tyr Trp Phe Gly Glu         
            180                 185                 190                 

ggc ggc gac atg acg ccg gac gag ttc ggc atc tgg gcg gcc gag caa      624
Gly Gly Asp Met Thr Pro Asp Glu Phe Gly Ile Trp Ala Ala Glu Gln         
        195                 200                 205                     

ctg gaa aag aaa att ctc gag ctg ggc gtc gag aac gtc ggt gcg ttc      672
Leu Glu Lys Lys Ile Leu Glu Leu Gly Val Glu Asn Val Gly Ala Phe         
    210                 215                 220                         

att gcc gag cca atc cag ggc gcg ggc ggt gtg att gtc ccg cct gat      720
Ile Ala Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro Pro Asp         
225                 230                 235                 240         

tcc tac tgg ccg aag atc aag gaa atc ctt tcc cgc tac gac atc ctg      768
Ser Tyr Trp Pro Lys Ile Lys Glu Ile Leu Ser Arg Tyr Asp Ile Leu         
                245                 250                 255             

ttc gcc gcc gat gag gtg att tgt ggc ttc ggg cgt acc agt gag tgg      816
Phe Ala Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Ser Glu Trp         
            260                 265                 270                 

ttc ggt agc gat ttc tat ggc ctc agg ccg gac atg atg acc atc gcc      864
Phe Gly Ser Asp Phe Tyr Gly Leu Arg Pro Asp Met Met Thr Ile Ala         
        275                 280                 285                     

aaa ggc ctg acc tcc ggt tac gta ccg atg ggc ggc ctg atc gtg cgc      912
Lys Gly Leu Thr Ser Gly Tyr Val Pro Met Gly Gly Leu Ile Val Arg         
    290                 295                 300                         

gat gaa atc gtt gcg gtg ctc aat gag ggt ggc gat ttc aat cac ggc      960
Asp Glu Ile Val Ala Val Leu Asn Glu Gly Gly Asp Phe Asn His Gly         
305                 310                 315                 320         

ttt acc tac tcc ggg cac ccg gtg gcg gcc gcg gtt gcg ctg gag aac     1008
Phe Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Ala Leu Glu Asn         
                325                 330                 335             

atc cgt atc ctg cgc gaa gaa aag atc gtc gaa cgg gtc agg tcg gaa     1056
Ile Arg Ile Leu Arg Glu Glu Lys Ile Val Glu Arg Val Arg Ser Glu         
            340                 345                 350                 

acg gca ccg tat ttg caa aag cgt ttg cgt gag ttg agc gat cat ccg     1104
Thr Ala Pro Tyr Leu Gln Lys Arg Leu Arg Glu Leu Ser Asp His Pro         
        355                 360                 365                     

ctg gtg ggc gaa gtc cgg ggt gtc ggg ctg ctc ggg gcc att gag ctg     1152
Leu Val Gly Glu Val Arg Gly Val Gly Leu Leu Gly Ala Ile Glu Leu         
    370                 375                 380                         

gtg aag gac aag acc acc cgc gag cgc tat acc gac aag ggc gcg gga     1200
Val Lys Asp Lys Thr Thr Arg Glu Arg Tyr Thr Asp Lys Gly Ala Gly         
385                 390                 395                 400         

atg atc tgt cga acc ttc tgc ttc gac aat ggc ctg atc atg cgg gct     1248
Met Ile Cys Arg Thr Phe Cys Phe Asp Asn Gly Leu Ile Met Arg Ala         
                405                 410                 415             

gtg ggc gat acc atg atc att gcg ccg cca ctg gtg atc agt ttt gcg     1296
Val Gly Asp Thr Met Ile Ile Ala Pro Pro Leu Val Ile Ser Phe Ala         
            420                 425                 430                 

caa atc gat gag ctg gta gag aag gcg cgc acg tgt ctg gat ctg acg     1344
Gln Ile Asp Glu Leu Val Glu Lys Ala Arg Thr Cys Leu Asp Leu Thr         
        435                 440                 445                     

ctg gcg gtg ttg cag ggc tga                                         1365
Leu Ala Val Leu Gln Gly                                                 
    450                                                                 


<210>  12
<211>  454
<212>  PRT
<213>  Pseudomonas syringae

<400>  12

Met Ser Ala Asn Asn Pro Gln Thr Leu Glu Trp Gln Ala Leu Ser Ser 
1               5                   10                  15      


Glu His His Leu Ala Pro Phe Ser Asp Tyr Lys Gln Leu Lys Glu Lys 
            20                  25                  30          


Gly Pro Arg Ile Ile Thr Arg Ala Glu Gly Val Tyr Leu Trp Asp Ser 
        35                  40                  45              


Glu Gly Asn Lys Ile Leu Asp Gly Met Ser Gly Leu Trp Cys Val Ala 
    50                  55                  60                  


Ile Gly Tyr Gly Arg Glu Glu Leu Ala Asp Ala Ala Ser Lys Gln Met 
65                  70                  75                  80  


Arg Glu Leu Pro Tyr Tyr Asn Leu Phe Phe Gln Thr Ala His Pro Pro 
                85                  90                  95      


Val Leu Glu Leu Ala Lys Ala Ile Ser Asp Ile Ala Pro Glu Gly Met 
            100                 105                 110         


Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Gly Asn Asp Thr Met 
        115                 120                 125             


Leu Arg Met Val Arg His Tyr Trp Ala Leu Lys Gly Gln Pro Asn Lys 
    130                 135                 140                 


Lys Thr Ile Ile Ser Arg Val Asn Gly Tyr His Gly Ser Thr Val Ala 
145                 150                 155                 160 


Gly Ala Ser Leu Gly Gly Met Thr Tyr Met His Glu Gln Gly Asp Leu 
                165                 170                 175     


Pro Ile Pro Gly Val Val His Ile Pro Gln Pro Tyr Trp Phe Gly Glu 
            180                 185                 190         


Gly Gly Asp Met Thr Pro Asp Glu Phe Gly Ile Trp Ala Ala Glu Gln 
        195                 200                 205             


Leu Glu Lys Lys Ile Leu Glu Leu Gly Val Glu Asn Val Gly Ala Phe 
    210                 215                 220                 


Ile Ala Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro Pro Asp 
225                 230                 235                 240 


Ser Tyr Trp Pro Lys Ile Lys Glu Ile Leu Ser Arg Tyr Asp Ile Leu 
                245                 250                 255     


Phe Ala Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Ser Glu Trp 
            260                 265                 270         


Phe Gly Ser Asp Phe Tyr Gly Leu Arg Pro Asp Met Met Thr Ile Ala 
        275                 280                 285             


Lys Gly Leu Thr Ser Gly Tyr Val Pro Met Gly Gly Leu Ile Val Arg 
    290                 295                 300                 


Asp Glu Ile Val Ala Val Leu Asn Glu Gly Gly Asp Phe Asn His Gly 
305                 310                 315                 320 


Phe Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Ala Leu Glu Asn 
                325                 330                 335     


Ile Arg Ile Leu Arg Glu Glu Lys Ile Val Glu Arg Val Arg Ser Glu 
            340                 345                 350         


Thr Ala Pro Tyr Leu Gln Lys Arg Leu Arg Glu Leu Ser Asp His Pro 
        355                 360                 365             


Leu Val Gly Glu Val Arg Gly Val Gly Leu Leu Gly Ala Ile Glu Leu 
    370                 375                 380                 


Val Lys Asp Lys Thr Thr Arg Glu Arg Tyr Thr Asp Lys Gly Ala Gly 
385                 390                 395                 400 


Met Ile Cys Arg Thr Phe Cys Phe Asp Asn Gly Leu Ile Met Arg Ala 
                405                 410                 415     


Val Gly Asp Thr Met Ile Ile Ala Pro Pro Leu Val Ile Ser Phe Ala 
            420                 425                 430         


Gln Ile Asp Glu Leu Val Glu Lys Ala Arg Thr Cys Leu Asp Leu Thr 
        435                 440                 445             


Leu Ala Val Leu Gln Gly 
    450                 


<210>  13
<211>  1365
<212>  DNA
<213>  Artificial

<220>
<223>  Pseudomonas syringae codon optimised aminotransferase gene

<400>  13
atgtctgcta acaatccaca aactctggaa tggcaggcac tgagctccga acatcacctg     60

gctccgttct ccgactacaa acaactgaaa gagaaaggcc cgcgtatcat tacccgcgct    120

gaaggtgtgt acctgtggga ttctgaaggc aacaaaattc tggacggtat gagcggcctg    180

tggtgcgtag caatcggtta tggccgtgaa gaactggctg acgcggcgag caaacagatg    240

cgtgaactgc cgtattataa cctgttcttc caaaccgcac acccgccggt tctggaactg    300

gctaaagcta tcagcgatat cgcaccggag ggcatgaatc acgtcttctt cactggttcc    360

ggtagcgaag gcaacgacac gatgctgcgc atggtacgtc actattgggc gctgaagggc    420

cagccgaaca agaaaacgat tatcagccgt gtaaacggtt atcacggcag caccgttgcg    480

ggtgcgagcc tgggcggtat gacctacatg cacgaacagg gtgacctgcc gatcccgggt    540

gtagtgcaca ttccgcagcc gtattggttc ggtgaaggcg gtgacatgac gccggacgaa    600

ttcggcatct gggcggcaga gcagctggaa aagaaaatcc tggaactggg cgtggaaaac    660

gtcggcgcgt tcatcgcgga accgattcag ggcgcgggcg gcgtaattgt tccgccggac    720

agctactggc caaaaatcaa agagatcctg tctcgttacg acatcctgtt cgccgcagac    780

gaagtgatct gcggttttgg ccgcacctct gaatggttcg gctccgactt ctacggtctg    840

cgtccggaca tgatgaccat cgccaaaggc ctgacctccg gttatgttcc tatgggtggc    900

ctgatcgtgc gcgacgaaat tgttgcggtt ctgaacgaag gcggcgattt caaccacggc    960

ttcacctatt ccggtcaccc agttgctgct gctgtagcac tggaaaacat ccgcatcctg   1020

cgtgaagaaa agatcgtaga acgcgtacgt tccgaaaccg caccttacct gcagaagcgc   1080

ctgcgcgaac tgagcgacca ccctctggta ggtgaagttc gcggcgtggg cctgctgggc   1140

gcgatcgagc tggtgaaaga caaaactacc cgtgaacgtt acaccgacaa aggcgcaggc   1200

atgatctgcc gtaccttttg cttcgataac ggtctgatca tgcgcgcagt cggtgatacc   1260

atgatcattg ctccgcctct ggttatttct tttgcccaga ttgatgagct ggtcgaaaaa   1320

gcgcgcactt gtctggatct gactctggct gttctgcagg gttaa                   1365


<210>  14
<211>  849
<212>  DNA
<213>  Bacillus subtilis (gi 16078032)


<220>
<221>  CDS
<222>  (1)..(849)

<400>  14
atg aag gtt tta gtc aat ggc cgg ctg att ggg cgc agt gaa gca tca       48
Met Lys Val Leu Val Asn Gly Arg Leu Ile Gly Arg Ser Glu Ala Ser         
1               5                   10                  15              

atc gat ttg gaa gat cgc ggt tat cag ttt ggt gac ggc atc tat gaa       96
Ile Asp Leu Glu Asp Arg Gly Tyr Gln Phe Gly Asp Gly Ile Tyr Glu         
            20                  25                  30                  

gtg atc agg gtg tac aaa gga gta ttg ttc ggc tta cgt gag cat gca      144
Val Ile Arg Val Tyr Lys Gly Val Leu Phe Gly Leu Arg Glu His Ala         
        35                  40                  45                      

gag cgt ttt ttc aga agt gct gct gaa atc gga att tca ctg cca ttc      192
Glu Arg Phe Phe Arg Ser Ala Ala Glu Ile Gly Ile Ser Leu Pro Phe         
    50                  55                  60                          

agt ata gaa gat ctc gag tgg gac ctg caa aag ctt gta cag gaa aat      240
Ser Ile Glu Asp Leu Glu Trp Asp Leu Gln Lys Leu Val Gln Glu Asn         
65                  70                  75                  80          

gcg gtc agt gag gga gcg gta tac att cag aca aca aga ggt gtg gcc      288
Ala Val Ser Glu Gly Ala Val Tyr Ile Gln Thr Thr Arg Gly Val Ala         
                85                  90                  95              

ccg cga aaa cac cag tat gaa gcc ggc ctc gag ccg cag act act gcc      336
Pro Arg Lys His Gln Tyr Glu Ala Gly Leu Glu Pro Gln Thr Thr Ala         
            100                 105                 110                 

tat acg ttt acg gtg aaa aaa ccg gag caa gag cag gca tac gga gtg      384
Tyr Thr Phe Thr Val Lys Lys Pro Glu Gln Glu Gln Ala Tyr Gly Val         
        115                 120                 125                     

gcg gcc att aca gat gag gat ctt cgc tgg tta aga tgt gat atc aaa      432
Ala Ala Ile Thr Asp Glu Asp Leu Arg Trp Leu Arg Cys Asp Ile Lys         
    130                 135                 140                         

agt ctg aat tta ctg tat aat gtc atg acg aag caa agg gcc tat gaa      480
Ser Leu Asn Leu Leu Tyr Asn Val Met Thr Lys Gln Arg Ala Tyr Glu         
145                 150                 155                 160         

gcc gga gca ttt gaa gcc att tta ctt agg gac ggc gtt gtt acg gag      528
Ala Gly Ala Phe Glu Ala Ile Leu Leu Arg Asp Gly Val Val Thr Glu         
                165                 170                 175             

ggt aca tcc tct aac gtt tat gcc gtt atc aac ggc aca gtg cga aca      576
Gly Thr Ser Ser Asn Val Tyr Ala Val Ile Asn Gly Thr Val Arg Thr         
            180                 185                 190                 

cat ccg gct aat cgg ctc att ctc aat gga att aca cgg atg aat att      624
His Pro Ala Asn Arg Leu Ile Leu Asn Gly Ile Thr Arg Met Asn Ile         
        195                 200                 205                     

tta gga ctg att gag aag aat ggg atc aaa ctg gat gag act cct gtc      672
Leu Gly Leu Ile Glu Lys Asn Gly Ile Lys Leu Asp Glu Thr Pro Val         
    210                 215                 220                         

agt gaa gaa gag ttg aaa cag gcg gaa gag atc ttt att tcg tca acg      720
Ser Glu Glu Glu Leu Lys Gln Ala Glu Glu Ile Phe Ile Ser Ser Thr         
225                 230                 235                 240         

acg gca gaa att att ccg gtc gtg acg ctc gat gga caa tcg atc gga      768
Thr Ala Glu Ile Ile Pro Val Val Thr Leu Asp Gly Gln Ser Ile Gly         
                245                 250                 255             

agc ggg aaa ccc gga ccg gtg acc aaa cag ctt cag gct gct ttt caa      816
Ser Gly Lys Pro Gly Pro Val Thr Lys Gln Leu Gln Ala Ala Phe Gln         
            260                 265                 270                 

gaa agc att caa cag gct gct agc att tca taa                          849
Glu Ser Ile Gln Gln Ala Ala Ser Ile Ser                                 
        275                 280                                         


<210>  15
<211>  282
<212>  PRT
<213>  Bacillus subtilis (gi 16078032)

<400>  15

Met Lys Val Leu Val Asn Gly Arg Leu Ile Gly Arg Ser Glu Ala Ser 
1               5                   10                  15      


Ile Asp Leu Glu Asp Arg Gly Tyr Gln Phe Gly Asp Gly Ile Tyr Glu 
            20                  25                  30          


Val Ile Arg Val Tyr Lys Gly Val Leu Phe Gly Leu Arg Glu His Ala 
        35                  40                  45              


Glu Arg Phe Phe Arg Ser Ala Ala Glu Ile Gly Ile Ser Leu Pro Phe 
    50                  55                  60                  


Ser Ile Glu Asp Leu Glu Trp Asp Leu Gln Lys Leu Val Gln Glu Asn 
65                  70                  75                  80  


Ala Val Ser Glu Gly Ala Val Tyr Ile Gln Thr Thr Arg Gly Val Ala 
                85                  90                  95      


Pro Arg Lys His Gln Tyr Glu Ala Gly Leu Glu Pro Gln Thr Thr Ala 
            100                 105                 110         


Tyr Thr Phe Thr Val Lys Lys Pro Glu Gln Glu Gln Ala Tyr Gly Val 
        115                 120                 125             


Ala Ala Ile Thr Asp Glu Asp Leu Arg Trp Leu Arg Cys Asp Ile Lys 
    130                 135                 140                 


Ser Leu Asn Leu Leu Tyr Asn Val Met Thr Lys Gln Arg Ala Tyr Glu 
145                 150                 155                 160 


Ala Gly Ala Phe Glu Ala Ile Leu Leu Arg Asp Gly Val Val Thr Glu 
                165                 170                 175     


Gly Thr Ser Ser Asn Val Tyr Ala Val Ile Asn Gly Thr Val Arg Thr 
            180                 185                 190         


His Pro Ala Asn Arg Leu Ile Leu Asn Gly Ile Thr Arg Met Asn Ile 
        195                 200                 205             


Leu Gly Leu Ile Glu Lys Asn Gly Ile Lys Leu Asp Glu Thr Pro Val 
    210                 215                 220                 


Ser Glu Glu Glu Leu Lys Gln Ala Glu Glu Ile Phe Ile Ser Ser Thr 
225                 230                 235                 240 


Thr Ala Glu Ile Ile Pro Val Val Thr Leu Asp Gly Gln Ser Ile Gly 
                245                 250                 255     


Ser Gly Lys Pro Gly Pro Val Thr Lys Gln Leu Gln Ala Ala Phe Gln 
            260                 265                 270         


Glu Ser Ile Gln Gln Ala Ala Ser Ile Ser 
        275                 280         


<210>  16
<211>  1347
<212>  DNA
<213>  Bacillus subtilis (gi 16080075)


<220>
<221>  CDS
<222>  (1)..(1347)

<400>  16
atg act cat gat ttg ata gaa aaa agt aaa aag cac ctc tgg ctg cca       48
Met Thr His Asp Leu Ile Glu Lys Ser Lys Lys His Leu Trp Leu Pro         
1               5                   10                  15              

ttt acc caa atg aaa gat tat gat gaa aac ccc tta atc atc gaa agc       96
Phe Thr Gln Met Lys Asp Tyr Asp Glu Asn Pro Leu Ile Ile Glu Ser         
            20                  25                  30                  

ggg act gga atc aaa gtc aaa gac ata aac ggc aag gaa tac tat gac      144
Gly Thr Gly Ile Lys Val Lys Asp Ile Asn Gly Lys Glu Tyr Tyr Asp         
        35                  40                  45                      

ggt ttt tca tcg gtt tgg ctt aat gtc cac gga cac cgc aaa aaa gaa      192
Gly Phe Ser Ser Val Trp Leu Asn Val His Gly His Arg Lys Lys Glu         
    50                  55                  60                          

cta gat gac gcc ata aaa aaa cag ctc gga aaa att gcg cac tcc acg      240
Leu Asp Asp Ala Ile Lys Lys Gln Leu Gly Lys Ile Ala His Ser Thr         
65                  70                  75                  80          

tta ttg ggc atg acc aat gtt cca gca acc cag ctt gcc gaa aca tta      288
Leu Leu Gly Met Thr Asn Val Pro Ala Thr Gln Leu Ala Glu Thr Leu         
                85                  90                  95              

atc gac atc agc cca aaa aag ctc acg cgg gtc ttt tat tca gac agc      336
Ile Asp Ile Ser Pro Lys Lys Leu Thr Arg Val Phe Tyr Ser Asp Ser         
            100                 105                 110                 

ggc gca gag gcg atg gaa ata gcc cta aaa atg gcg ttt cag tat tgg      384
Gly Ala Glu Ala Met Glu Ile Ala Leu Lys Met Ala Phe Gln Tyr Trp         
        115                 120                 125                     

aag aac atc ggg aag ccc gag aaa caa aaa ttc atc gca atg aaa aac      432
Lys Asn Ile Gly Lys Pro Glu Lys Gln Lys Phe Ile Ala Met Lys Asn         
    130                 135                 140                         

ggg tat cac ggt gat acg att ggc gcc gtc agt gtc ggt tca att gag      480
Gly Tyr His Gly Asp Thr Ile Gly Ala Val Ser Val Gly Ser Ile Glu         
145                 150                 155                 160         

ctt ttt cac cac gta tac ggc ccg ttg atg ttc gag agt tac aag gcc      528
Leu Phe His His Val Tyr Gly Pro Leu Met Phe Glu Ser Tyr Lys Ala         
                165                 170                 175             

ccg att cct tat gtg tat cgt tct gaa agc ggt gat cct gat gag tgc      576
Pro Ile Pro Tyr Val Tyr Arg Ser Glu Ser Gly Asp Pro Asp Glu Cys         
            180                 185                 190                 

cgt gat cag tgc ctc cga gag ctt gca cag ctg ctt gag gaa cat cat      624
Arg Asp Gln Cys Leu Arg Glu Leu Ala Gln Leu Leu Glu Glu His His         
        195                 200                 205                     

gag gaa att gcc gcg ctt tcc att gaa tca atg gta caa ggc gcg tcc      672
Glu Glu Ile Ala Ala Leu Ser Ile Glu Ser Met Val Gln Gly Ala Ser         
    210                 215                 220                         

ggt atg atc gtg atg ccg gaa gga tat ttg gca ggc gtg cgc gag cta      720
Gly Met Ile Val Met Pro Glu Gly Tyr Leu Ala Gly Val Arg Glu Leu         
225                 230                 235                 240         

tgt aca aca tac gat gtc tta atg atc gtt gat gaa gtc gct aca ggc      768
Cys Thr Thr Tyr Asp Val Leu Met Ile Val Asp Glu Val Ala Thr Gly         
                245                 250                 255             

ttt ggc cgt aca gga aaa atg ttt gcg tgc gag cac gag aat gtc cag      816
Phe Gly Arg Thr Gly Lys Met Phe Ala Cys Glu His Glu Asn Val Gln         
            260                 265                 270                 

cct gat ctg atg gct gcc ggt aaa ggc att aca gga ggc tat ttg cca      864
Pro Asp Leu Met Ala Ala Gly Lys Gly Ile Thr Gly Gly Tyr Leu Pro         
        275                 280                 285                     

att gcc gtt acg ttt gcc act gaa gac atc tat aag gca ttc tat gat      912
Ile Ala Val Thr Phe Ala Thr Glu Asp Ile Tyr Lys Ala Phe Tyr Asp         
    290                 295                 300                         

gat tat gaa aac cta aaa acc ttt ttc cat ggc cat tcc tat aca ggc      960
Asp Tyr Glu Asn Leu Lys Thr Phe Phe His Gly His Ser Tyr Thr Gly         
305                 310                 315                 320         

aat cag ctt ggc tgt gcg gtt gcg ctt gaa aat ctg gca tta ttt gaa     1008
Asn Gln Leu Gly Cys Ala Val Ala Leu Glu Asn Leu Ala Leu Phe Glu         
                325                 330                 335             

tct gaa aac att gtg gaa caa gta gcg gaa aaa agt aaa aag ctc cat     1056
Ser Glu Asn Ile Val Glu Gln Val Ala Glu Lys Ser Lys Lys Leu His         
            340                 345                 350                 

ttt ctt ctt caa gat ctg cac gct ctt cct cat gtt ggg gat att cgg     1104
Phe Leu Leu Gln Asp Leu His Ala Leu Pro His Val Gly Asp Ile Arg         
        355                 360                 365                     

cag ctt ggc ttt atg tgc ggt gca gag ctt gta cga tca aag gaa act     1152
Gln Leu Gly Phe Met Cys Gly Ala Glu Leu Val Arg Ser Lys Glu Thr         
    370                 375                 380                         

aaa gaa cct tac ccg gct gat cgg cgg att gga tac aaa gtt tcc tta     1200
Lys Glu Pro Tyr Pro Ala Asp Arg Arg Ile Gly Tyr Lys Val Ser Leu         
385                 390                 395                 400         

aaa atg aga gag tta gga atg ctg aca aga ccg ctt ggg gac gtg att     1248
Lys Met Arg Glu Leu Gly Met Leu Thr Arg Pro Leu Gly Asp Val Ile         
                405                 410                 415             

gca ttt ctt cct cct ctt gcc agc aca gct gaa gag ctc tcg gaa atg     1296
Ala Phe Leu Pro Pro Leu Ala Ser Thr Ala Glu Glu Leu Ser Glu Met         
            420                 425                 430                 

gtt gcc att atg aaa caa gcg atc cac gag gtt acg agc ctt gaa gat     1344
Val Ala Ile Met Lys Gln Ala Ile His Glu Val Thr Ser Leu Glu Asp         
        435                 440                 445                     

tga                                                                 1347


<210>  17
<211>  448
<212>  PRT
<213>  Bacillus subtilis (gi 16080075)

<400>  17

Met Thr His Asp Leu Ile Glu Lys Ser Lys Lys His Leu Trp Leu Pro 
1               5                   10                  15      


Phe Thr Gln Met Lys Asp Tyr Asp Glu Asn Pro Leu Ile Ile Glu Ser 
            20                  25                  30          


Gly Thr Gly Ile Lys Val Lys Asp Ile Asn Gly Lys Glu Tyr Tyr Asp 
        35                  40                  45              


Gly Phe Ser Ser Val Trp Leu Asn Val His Gly His Arg Lys Lys Glu 
    50                  55                  60                  


Leu Asp Asp Ala Ile Lys Lys Gln Leu Gly Lys Ile Ala His Ser Thr 
65                  70                  75                  80  


Leu Leu Gly Met Thr Asn Val Pro Ala Thr Gln Leu Ala Glu Thr Leu 
                85                  90                  95      


Ile Asp Ile Ser Pro Lys Lys Leu Thr Arg Val Phe Tyr Ser Asp Ser 
            100                 105                 110         


Gly Ala Glu Ala Met Glu Ile Ala Leu Lys Met Ala Phe Gln Tyr Trp 
        115                 120                 125             


Lys Asn Ile Gly Lys Pro Glu Lys Gln Lys Phe Ile Ala Met Lys Asn 
    130                 135                 140                 


Gly Tyr His Gly Asp Thr Ile Gly Ala Val Ser Val Gly Ser Ile Glu 
145                 150                 155                 160 


Leu Phe His His Val Tyr Gly Pro Leu Met Phe Glu Ser Tyr Lys Ala 
                165                 170                 175     


Pro Ile Pro Tyr Val Tyr Arg Ser Glu Ser Gly Asp Pro Asp Glu Cys 
            180                 185                 190         


Arg Asp Gln Cys Leu Arg Glu Leu Ala Gln Leu Leu Glu Glu His His 
        195                 200                 205             


Glu Glu Ile Ala Ala Leu Ser Ile Glu Ser Met Val Gln Gly Ala Ser 
    210                 215                 220                 


Gly Met Ile Val Met Pro Glu Gly Tyr Leu Ala Gly Val Arg Glu Leu 
225                 230                 235                 240 


Cys Thr Thr Tyr Asp Val Leu Met Ile Val Asp Glu Val Ala Thr Gly 
                245                 250                 255     


Phe Gly Arg Thr Gly Lys Met Phe Ala Cys Glu His Glu Asn Val Gln 
            260                 265                 270         


Pro Asp Leu Met Ala Ala Gly Lys Gly Ile Thr Gly Gly Tyr Leu Pro 
        275                 280                 285             


Ile Ala Val Thr Phe Ala Thr Glu Asp Ile Tyr Lys Ala Phe Tyr Asp 
    290                 295                 300                 


Asp Tyr Glu Asn Leu Lys Thr Phe Phe His Gly His Ser Tyr Thr Gly 
305                 310                 315                 320 


Asn Gln Leu Gly Cys Ala Val Ala Leu Glu Asn Leu Ala Leu Phe Glu 
                325                 330                 335     


Ser Glu Asn Ile Val Glu Gln Val Ala Glu Lys Ser Lys Lys Leu His 
            340                 345                 350         


Phe Leu Leu Gln Asp Leu His Ala Leu Pro His Val Gly Asp Ile Arg 
        355                 360                 365             


Gln Leu Gly Phe Met Cys Gly Ala Glu Leu Val Arg Ser Lys Glu Thr 
    370                 375                 380                 


Lys Glu Pro Tyr Pro Ala Asp Arg Arg Ile Gly Tyr Lys Val Ser Leu 
385                 390                 395                 400 


Lys Met Arg Glu Leu Gly Met Leu Thr Arg Pro Leu Gly Asp Val Ile 
                405                 410                 415     


Ala Phe Leu Pro Pro Leu Ala Ser Thr Ala Glu Glu Leu Ser Glu Met 
            420                 425                 430         


Val Ala Ile Met Lys Gln Ala Ile His Glu Val Thr Ser Leu Glu Asp 
        435                 440                 445             


<210>  18
<211>  1467
<212>  DNA
<213>  Rhodobacter sphaeroides


<220>
<221>  CDS
<222>  (1)..(1467)

<400>  18
atg ccc ggt tgc ggg ggc ttg ccc ggg aat gaa ccg aaa tgc gga cga       48
Met Pro Gly Cys Gly Gly Leu Pro Gly Asn Glu Pro Lys Cys Gly Arg         
1               5                   10                  15              

gag ggg agg tcg gcg atg acg cgg aat gac gcg acg aat gct gcc gga       96
Glu Gly Arg Ser Ala Met Thr Arg Asn Asp Ala Thr Asn Ala Ala Gly         
            20                  25                  30                  

gcg gtg ggc gcg gcg atg cgg gat cac atc ctc ttg cct gca cag gaa      144
Ala Val Gly Ala Ala Met Arg Asp His Ile Leu Leu Pro Ala Gln Glu         
        35                  40                  45                      

atg gcg aag ctc ggc aag tcc gcg cag ccg gtg ctg act cat gcc gag      192
Met Ala Lys Leu Gly Lys Ser Ala Gln Pro Val Leu Thr His Ala Glu         
    50                  55                  60                          

ggc atc tat gtc cat acc gag gac ggc cgc cgc ctg atc gac ggg ccg      240
Gly Ile Tyr Val His Thr Glu Asp Gly Arg Arg Leu Ile Asp Gly Pro         
65                  70                  75                  80          

gcg ggc atg tgg tgc gcg cag gtg ggc tac ggc cgc cgc gag atc gtc      288
Ala Gly Met Trp Cys Ala Gln Val Gly Tyr Gly Arg Arg Glu Ile Val         
                85                  90                  95              

gat gcc atg gcg cat cag gcg atg gtg ctg ccc tat gcc tcg ccc tgg      336
Asp Ala Met Ala His Gln Ala Met Val Leu Pro Tyr Ala Ser Pro Trp         
            100                 105                 110                 

tat atg gcc acg agc ccc gcg gcg cgg ctg gcg gag aag atc gcc acg      384
Tyr Met Ala Thr Ser Pro Ala Ala Arg Leu Ala Glu Lys Ile Ala Thr         
        115                 120                 125                     

ctg acg ccg ggc gat ctc aac cgg atc ttt ttc acc acg ggc ggg tcg      432
Leu Thr Pro Gly Asp Leu Asn Arg Ile Phe Phe Thr Thr Gly Gly Ser         
    130                 135                 140                         

acc gcg gtg gac agc gcg ctg cgc ttc tcg gaa ttc tac aac aac gtg      480
Thr Ala Val Asp Ser Ala Leu Arg Phe Ser Glu Phe Tyr Asn Asn Val         
145                 150                 155                 160         

ctg ggc cgg ccg cag aag aag cgc atc atc gtg cgc tac gac ggc tat      528
Leu Gly Arg Pro Gln Lys Lys Arg Ile Ile Val Arg Tyr Asp Gly Tyr         
                165                 170                 175             

cac ggc tcg acg gcg ctc acc gcc gcc tgc acc ggc cgc acc ggc aac      576
His Gly Ser Thr Ala Leu Thr Ala Ala Cys Thr Gly Arg Thr Gly Asn         
            180                 185                 190                 

tgg ccg aac ttc gac atc gcg cag gac cgg atc tcg ttc ctc tcg agc      624
Trp Pro Asn Phe Asp Ile Ala Gln Asp Arg Ile Ser Phe Leu Ser Ser         
        195                 200                 205                     

ccc aat ccg cgc cac gcc ggc aac cgc agc cag gag gcg ttc ctc gac      672
Pro Asn Pro Arg His Ala Gly Asn Arg Ser Gln Glu Ala Phe Leu Asp         
    210                 215                 220                         

gat ctg gtg cag gaa ttc gag gac cgg atc gag agc ctc ggc ccc gac      720
Asp Leu Val Gln Glu Phe Glu Asp Arg Ile Glu Ser Leu Gly Pro Asp         
225                 230                 235                 240         

acg atc gcg gcc ttc ctg gcc gag ccg atc ctc gcc tcg ggc ggc gtc      768
Thr Ile Ala Ala Phe Leu Ala Glu Pro Ile Leu Ala Ser Gly Gly Val         
                245                 250                 255             

att att ccg ccc gca ggc tat cat gcg cgc ttc aag gcg atc tgc gag      816
Ile Ile Pro Pro Ala Gly Tyr His Ala Arg Phe Lys Ala Ile Cys Glu         
            260                 265                 270                 

aag cac gac atc ctc tat atc tcg gac gag gtg gtg acg ggc ttc ggc      864
Lys His Asp Ile Leu Tyr Ile Ser Asp Glu Val Val Thr Gly Phe Gly         
        275                 280                 285                     

cgt tgc ggc gag tgg ttc gcc tcg gag aag gtg ttc ggg gtg gtg ccg      912
Arg Cys Gly Glu Trp Phe Ala Ser Glu Lys Val Phe Gly Val Val Pro         
    290                 295                 300                         

gac atc atc acc ttc gcc aag ggc gtg acc tcg ggc tat gtg ccg ctc      960
Asp Ile Ile Thr Phe Ala Lys Gly Val Thr Ser Gly Tyr Val Pro Leu         
305                 310                 315                 320         

ggc ggc ctt gcg atc tcc gag gcg gtg ctg gcg cgg atc tcg ggc gag     1008
Gly Gly Leu Ala Ile Ser Glu Ala Val Leu Ala Arg Ile Ser Gly Glu         
                325                 330                 335             

aat gcc aag gga agc tgg ttc acc aac ggc tat acc tac agc aat cag     1056
Asn Ala Lys Gly Ser Trp Phe Thr Asn Gly Tyr Thr Tyr Ser Asn Gln         
            340                 345                 350                 

ccg gtg gcc tgc gcc gcg gcg ctt gcc aac atc gag ctg atg gag cgc     1104
Pro Val Ala Cys Ala Ala Ala Leu Ala Asn Ile Glu Leu Met Glu Arg         
        355                 360                 365                     

gag ggc atc gtc gat cag gcg cgc gag atg gcg gac tat ttc gcc gcg     1152
Glu Gly Ile Val Asp Gln Ala Arg Glu Met Ala Asp Tyr Phe Ala Ala         
    370                 375                 380                         

gcg ctg gct tcg ctg cgc gat ctg ccg ggc gtg gcg gaa acc cgg tcg     1200
Ala Leu Ala Ser Leu Arg Asp Leu Pro Gly Val Ala Glu Thr Arg Ser         
385                 390                 395                 400         

gtg ggc ctc gtg ggt tgc gtg caa tgc ctg ctc gac ccg acc cgg gcg     1248
Val Gly Leu Val Gly Cys Val Gln Cys Leu Leu Asp Pro Thr Arg Ala         
                405                 410                 415             

gac ggc acg gcc gag gac aag gcc ttc acc ctg aag atc gac gag cgc     1296
Asp Gly Thr Ala Glu Asp Lys Ala Phe Thr Leu Lys Ile Asp Glu Arg         
            420                 425                 430                 

tgc ttc gag ctc ggg ctg atc gtg cgc ccg ctg ggc gat ctc tgc gtg     1344
Cys Phe Glu Leu Gly Leu Ile Val Arg Pro Leu Gly Asp Leu Cys Val         
        435                 440                 445                     

atc tcg ccg ccg ctc atc atc tcg cgc gcg cag atc gac gag atg gtc     1392
Ile Ser Pro Pro Leu Ile Ile Ser Arg Ala Gln Ile Asp Glu Met Val         
    450                 455                 460                         

gcg atc atg cgg cag gcc atc acc gaa gtg agc gcc gcc cac ggt ctg     1440
Ala Ile Met Arg Gln Ala Ile Thr Glu Val Ser Ala Ala His Gly Leu         
465                 470                 475                 480         

acc gcg aaa gaa ccg gcc gcc gtc tga                                 1467
Thr Ala Lys Glu Pro Ala Ala Val                                         
                485                                                     


<210>  19
<211>  488
<212>  PRT
<213>  Rhodobacter sphaeroides

<400>  19

Met Pro Gly Cys Gly Gly Leu Pro Gly Asn Glu Pro Lys Cys Gly Arg 
1               5                   10                  15      


Glu Gly Arg Ser Ala Met Thr Arg Asn Asp Ala Thr Asn Ala Ala Gly 
            20                  25                  30          


Ala Val Gly Ala Ala Met Arg Asp His Ile Leu Leu Pro Ala Gln Glu 
        35                  40                  45              


Met Ala Lys Leu Gly Lys Ser Ala Gln Pro Val Leu Thr His Ala Glu 
    50                  55                  60                  


Gly Ile Tyr Val His Thr Glu Asp Gly Arg Arg Leu Ile Asp Gly Pro 
65                  70                  75                  80  


Ala Gly Met Trp Cys Ala Gln Val Gly Tyr Gly Arg Arg Glu Ile Val 
                85                  90                  95      


Asp Ala Met Ala His Gln Ala Met Val Leu Pro Tyr Ala Ser Pro Trp 
            100                 105                 110         


Tyr Met Ala Thr Ser Pro Ala Ala Arg Leu Ala Glu Lys Ile Ala Thr 
        115                 120                 125             


Leu Thr Pro Gly Asp Leu Asn Arg Ile Phe Phe Thr Thr Gly Gly Ser 
    130                 135                 140                 


Thr Ala Val Asp Ser Ala Leu Arg Phe Ser Glu Phe Tyr Asn Asn Val 
145                 150                 155                 160 


Leu Gly Arg Pro Gln Lys Lys Arg Ile Ile Val Arg Tyr Asp Gly Tyr 
                165                 170                 175     


His Gly Ser Thr Ala Leu Thr Ala Ala Cys Thr Gly Arg Thr Gly Asn 
            180                 185                 190         


Trp Pro Asn Phe Asp Ile Ala Gln Asp Arg Ile Ser Phe Leu Ser Ser 
        195                 200                 205             


Pro Asn Pro Arg His Ala Gly Asn Arg Ser Gln Glu Ala Phe Leu Asp 
    210                 215                 220                 


Asp Leu Val Gln Glu Phe Glu Asp Arg Ile Glu Ser Leu Gly Pro Asp 
225                 230                 235                 240 


Thr Ile Ala Ala Phe Leu Ala Glu Pro Ile Leu Ala Ser Gly Gly Val 
                245                 250                 255     


Ile Ile Pro Pro Ala Gly Tyr His Ala Arg Phe Lys Ala Ile Cys Glu 
            260                 265                 270         


Lys His Asp Ile Leu Tyr Ile Ser Asp Glu Val Val Thr Gly Phe Gly 
        275                 280                 285             


Arg Cys Gly Glu Trp Phe Ala Ser Glu Lys Val Phe Gly Val Val Pro 
    290                 295                 300                 


Asp Ile Ile Thr Phe Ala Lys Gly Val Thr Ser Gly Tyr Val Pro Leu 
305                 310                 315                 320 


Gly Gly Leu Ala Ile Ser Glu Ala Val Leu Ala Arg Ile Ser Gly Glu 
                325                 330                 335     


Asn Ala Lys Gly Ser Trp Phe Thr Asn Gly Tyr Thr Tyr Ser Asn Gln 
            340                 345                 350         


Pro Val Ala Cys Ala Ala Ala Leu Ala Asn Ile Glu Leu Met Glu Arg 
        355                 360                 365             


Glu Gly Ile Val Asp Gln Ala Arg Glu Met Ala Asp Tyr Phe Ala Ala 
    370                 375                 380                 


Ala Leu Ala Ser Leu Arg Asp Leu Pro Gly Val Ala Glu Thr Arg Ser 
385                 390                 395                 400 


Val Gly Leu Val Gly Cys Val Gln Cys Leu Leu Asp Pro Thr Arg Ala 
                405                 410                 415     


Asp Gly Thr Ala Glu Asp Lys Ala Phe Thr Leu Lys Ile Asp Glu Arg 
            420                 425                 430         


Cys Phe Glu Leu Gly Leu Ile Val Arg Pro Leu Gly Asp Leu Cys Val 
        435                 440                 445             


Ile Ser Pro Pro Leu Ile Ile Ser Arg Ala Gln Ile Asp Glu Met Val 
    450                 455                 460                 


Ala Ile Met Arg Gln Ala Ile Thr Glu Val Ser Ala Ala His Gly Leu 
465                 470                 475                 480 


Thr Ala Lys Glu Pro Ala Ala Val 
                485             


<210>  20
<211>  837
<212>  DNA
<213>  Legionella pneumophila


<220>
<221>  CDS
<222>  (1)..(837)

<400>  20
atg agt atc gca ttt gtt aac ggc aag tat tgt tgt caa tct gaa gca       48
Met Ser Ile Ala Phe Val Asn Gly Lys Tyr Cys Cys Gln Ser Glu Ala         
1               5                   10                  15              

aaa att tca ata ttt gat cga ggg ttt ctt ttt ggt gac tcg gtt tat       96
Lys Ile Ser Ile Phe Asp Arg Gly Phe Leu Phe Gly Asp Ser Val Tyr         
            20                  25                  30                  

gaa gtg ctg cct gtt tac cat ggg cag cct tac ttt gta gac caa cat      144
Glu Val Leu Pro Val Tyr His Gly Gln Pro Tyr Phe Val Asp Gln His         
        35                  40                  45                      

ctt gac cga tta ttc tca aat atg aaa aaa att aag atg att ata cca      192
Leu Asp Arg Leu Phe Ser Asn Met Lys Lys Ile Lys Met Ile Ile Pro         
    50                  55                  60                          

aat tat gat tgg cat ggt tta att cat aga cta ata tca gaa aat aat      240
Asn Tyr Asp Trp His Gly Leu Ile His Arg Leu Ile Ser Glu Asn Asn         
65                  70                  75                  80          

ggc ggt aat tta caa gta tat atc caa gtc aca cga ggg aat caa ggg      288
Gly Gly Asn Leu Gln Val Tyr Ile Gln Val Thr Arg Gly Asn Gln Gly         
                85                  90                  95              

gtg cgc aag cat gat atc cct act tcc atc aca cct tct gtt atc gca      336
Val Arg Lys His Asp Ile Pro Thr Ser Ile Thr Pro Ser Val Ile Ala         
            100                 105                 110                 

ttc act atg cat aat cca ttt ccc acc ctc gaa gat aag gaa cag gga      384
Phe Thr Met His Asn Pro Phe Pro Thr Leu Glu Asp Lys Glu Gln Gly         
        115                 120                 125                     

atg tca gca aaa ctg gtt gaa gat ttt cgg tgg atg aga tgt gat ata      432
Met Ser Ala Lys Leu Val Glu Asp Phe Arg Trp Met Arg Cys Asp Ile         
    130                 135                 140                         

aaa act act tct tta att gcc aat ata tta ctg aat gat gag gct gta      480
Lys Thr Thr Ser Leu Ile Ala Asn Ile Leu Leu Asn Asp Glu Ala Val         
145                 150                 155                 160         

tct gca gga ttc cac act gca att ctt gcc cgg aac ggt cta att aca      528
Ser Ala Gly Phe His Thr Ala Ile Leu Ala Arg Asn Gly Leu Ile Thr         
                165                 170                 175             

gag gga agt agt acc aac gta ttt att gtc gca cag gat ggt gtt att      576
Glu Gly Ser Ser Thr Asn Val Phe Ile Val Ala Gln Asp Gly Val Ile         
            180                 185                 190                 

aag aca cca ccc atg aat aat ttc tgt tta cca gga att act cgg caa      624
Lys Thr Pro Pro Met Asn Asn Phe Cys Leu Pro Gly Ile Thr Arg Gln         
        195                 200                 205                     

gtt gtt att gaa ata att aaa aaa tta gat tta aag ttc aga gaa ata      672
Val Val Ile Glu Ile Ile Lys Lys Leu Asp Leu Lys Phe Arg Glu Ile         
    210                 215                 220                         

gaa att agc att tca gag ctt ttt tct gct cag gaa gtt tgg ata aca      720
Glu Ile Ser Ile Ser Glu Leu Phe Ser Ala Gln Glu Val Trp Ile Thr         
225                 230                 235                 240         

agt acg aca aaa gaa gta ttc cct att aca aag att aat gac tct ttg      768
Ser Thr Thr Lys Glu Val Phe Pro Ile Thr Lys Ile Asn Asp Ser Leu         
                245                 250                 255             

att aat ggc gga aaa gtt ggc gaa tat tgg cgg ata att aat gat tcc      816
Ile Asn Gly Gly Lys Val Gly Glu Tyr Trp Arg Ile Ile Asn Asp Ser         
            260                 265                 270                 

tac caa caa cta gta aac taa                                          837
Tyr Gln Gln Leu Val Asn                                                 
        275                                                             


<210>  21
<211>  278
<212>  PRT
<213>  Legionella pneumophila

<400>  21

Met Ser Ile Ala Phe Val Asn Gly Lys Tyr Cys Cys Gln Ser Glu Ala 
1               5                   10                  15      


Lys Ile Ser Ile Phe Asp Arg Gly Phe Leu Phe Gly Asp Ser Val Tyr 
            20                  25                  30          


Glu Val Leu Pro Val Tyr His Gly Gln Pro Tyr Phe Val Asp Gln His 
        35                  40                  45              


Leu Asp Arg Leu Phe Ser Asn Met Lys Lys Ile Lys Met Ile Ile Pro 
    50                  55                  60                  


Asn Tyr Asp Trp His Gly Leu Ile His Arg Leu Ile Ser Glu Asn Asn 
65                  70                  75                  80  


Gly Gly Asn Leu Gln Val Tyr Ile Gln Val Thr Arg Gly Asn Gln Gly 
                85                  90                  95      


Val Arg Lys His Asp Ile Pro Thr Ser Ile Thr Pro Ser Val Ile Ala 
            100                 105                 110         


Phe Thr Met His Asn Pro Phe Pro Thr Leu Glu Asp Lys Glu Gln Gly 
        115                 120                 125             


Met Ser Ala Lys Leu Val Glu Asp Phe Arg Trp Met Arg Cys Asp Ile 
    130                 135                 140                 


Lys Thr Thr Ser Leu Ile Ala Asn Ile Leu Leu Asn Asp Glu Ala Val 
145                 150                 155                 160 


Ser Ala Gly Phe His Thr Ala Ile Leu Ala Arg Asn Gly Leu Ile Thr 
                165                 170                 175     


Glu Gly Ser Ser Thr Asn Val Phe Ile Val Ala Gln Asp Gly Val Ile 
            180                 185                 190         


Lys Thr Pro Pro Met Asn Asn Phe Cys Leu Pro Gly Ile Thr Arg Gln 
        195                 200                 205             


Val Val Ile Glu Ile Ile Lys Lys Leu Asp Leu Lys Phe Arg Glu Ile 
    210                 215                 220                 


Glu Ile Ser Ile Ser Glu Leu Phe Ser Ala Gln Glu Val Trp Ile Thr 
225                 230                 235                 240 


Ser Thr Thr Lys Glu Val Phe Pro Ile Thr Lys Ile Asn Asp Ser Leu 
                245                 250                 255     


Ile Asn Gly Gly Lys Val Gly Glu Tyr Trp Arg Ile Ile Asn Asp Ser 
            260                 265                 270         


Tyr Gln Gln Leu Val Asn 
        275             


<210>  22
<211>  861
<212>  DNA
<213>  Nitrosomonas europaea


<220>
<221>  CDS
<222>  (1)..(861)

<400>  22
atg att tac ctc aat ggc aaa ttt ctg ccg atg gaa cag gct acc gtt       48
Met Ile Tyr Leu Asn Gly Lys Phe Leu Pro Met Glu Gln Ala Thr Val         
1               5                   10                  15              

cca gtg ctg gat aga ggc ttc atc ttc ggt gat ggt gtc tat gaa gtc       96
Pro Val Leu Asp Arg Gly Phe Ile Phe Gly Asp Gly Val Tyr Glu Val         
            20                  25                  30                  

ata ccg gtt tat tca cgt aaa ccg ttc cgg ctg ggc gaa cat ctt tcc      144
Ile Pro Val Tyr Ser Arg Lys Pro Phe Arg Leu Gly Glu His Leu Ser         
        35                  40                  45                      

cgg ctg cag cac agt ctg gat ggc ata cgt ctc cag aat ccg cac act      192
Arg Leu Gln His Ser Leu Asp Gly Ile Arg Leu Gln Asn Pro His Thr         
    50                  55                  60                          

gaa gaa caa tgg gct ggt ctg atc gaa cgc atc atc gag ctg aat gaa      240
Glu Glu Gln Trp Ala Gly Leu Ile Glu Arg Ile Ile Glu Leu Asn Glu         
65                  70                  75                  80          

ggt gat gat cag tac ctt tac ctg cac att aca cgc ggg gtg gca aaa      288
Gly Asp Asp Gln Tyr Leu Tyr Leu His Ile Thr Arg Gly Val Ala Lys         
                85                  90                  95              

cgt gac cat gcc ttt cct cgc gaa gta acg ccc act gtc ttc atc atg      336
Arg Asp His Ala Phe Pro Arg Glu Val Thr Pro Thr Val Phe Ile Met         
            100                 105                 110                 

agc aac ccg ctt ccg gct cca cct gca aaa ttg ctc gtt tcc gga gtt      384
Ser Asn Pro Leu Pro Ala Pro Pro Ala Lys Leu Leu Val Ser Gly Val         
        115                 120                 125                     

tca gcg att acc gcc agg gat aat cgc tgg ggg cgc tgt gat atc aaa      432
Ser Ala Ile Thr Ala Arg Asp Asn Arg Trp Gly Arg Cys Asp Ile Lys         
    130                 135                 140                         

gcc att tca ctg ttg cca aat atc tta ttg cgc cag ctt gcc gtg gac      480
Ala Ile Ser Leu Leu Pro Asn Ile Leu Leu Arg Gln Leu Ala Val Asp         
145                 150                 155                 160         

gca caa gcc atg gaa acg atc ctg tta cgc gat ggt ctg ttg acc gaa      528
Ala Gln Ala Met Glu Thr Ile Leu Leu Arg Asp Gly Leu Leu Thr Glu         
                165                 170                 175             

ggg gcc gcc agc aat att ttc atc gta aaa gac gac ctg ctg ctg acc      576
Gly Ala Ala Ser Asn Ile Phe Ile Val Lys Asp Asp Leu Leu Leu Thr         
            180                 185                 190                 

ccc ccc aaa gat cac cgt ata ttg cct ggc att act tat gat gta gta      624
Pro Pro Lys Asp His Arg Ile Leu Pro Gly Ile Thr Tyr Asp Val Val         
        195                 200                 205                     

ctg gaa ctg gct gaa aca cat ggt gtt cca cat gcg aca aga gaa ata      672
Leu Glu Leu Ala Glu Thr His Gly Val Pro His Ala Thr Arg Glu Ile         
    210                 215                 220                         

tca gag ctt gag tta cgt act gca cgg gaa atc atg ctg act tct tcc      720
Ser Glu Leu Glu Leu Arg Thr Ala Arg Glu Ile Met Leu Thr Ser Ser         
225                 230                 235                 240         

acc aaa gaa att ctc ccg atc aca cag ctg gat gga caa ccg atc ggt      768
Thr Lys Glu Ile Leu Pro Ile Thr Gln Leu Asp Gly Gln Pro Ile Gly         
                245                 250                 255             

aat ggc acc cca ggg cca gta ttt cag caa ctg gat cgg ctc tat cag      816
Asn Gly Thr Pro Gly Pro Val Phe Gln Gln Leu Asp Arg Leu Tyr Gln         
            260                 265                 270                 

gca tat aag ctg gaa gtc atg cgc ggg cat gct cca cgc cag taa          861
Ala Tyr Lys Leu Glu Val Met Arg Gly His Ala Pro Arg Gln                 
        275                 280                 285                     


<210>  23
<211>  286
<212>  PRT
<213>  Nitrosomonas europaea

<400>  23

Met Ile Tyr Leu Asn Gly Lys Phe Leu Pro Met Glu Gln Ala Thr Val 
1               5                   10                  15      


Pro Val Leu Asp Arg Gly Phe Ile Phe Gly Asp Gly Val Tyr Glu Val 
            20                  25                  30          


Ile Pro Val Tyr Ser Arg Lys Pro Phe Arg Leu Gly Glu His Leu Ser 
        35                  40                  45              


Arg Leu Gln His Ser Leu Asp Gly Ile Arg Leu Gln Asn Pro His Thr 
    50                  55                  60                  


Glu Glu Gln Trp Ala Gly Leu Ile Glu Arg Ile Ile Glu Leu Asn Glu 
65                  70                  75                  80  


Gly Asp Asp Gln Tyr Leu Tyr Leu His Ile Thr Arg Gly Val Ala Lys 
                85                  90                  95      


Arg Asp His Ala Phe Pro Arg Glu Val Thr Pro Thr Val Phe Ile Met 
            100                 105                 110         


Ser Asn Pro Leu Pro Ala Pro Pro Ala Lys Leu Leu Val Ser Gly Val 
        115                 120                 125             


Ser Ala Ile Thr Ala Arg Asp Asn Arg Trp Gly Arg Cys Asp Ile Lys 
    130                 135                 140                 


Ala Ile Ser Leu Leu Pro Asn Ile Leu Leu Arg Gln Leu Ala Val Asp 
145                 150                 155                 160 


Ala Gln Ala Met Glu Thr Ile Leu Leu Arg Asp Gly Leu Leu Thr Glu 
                165                 170                 175     


Gly Ala Ala Ser Asn Ile Phe Ile Val Lys Asp Asp Leu Leu Leu Thr 
            180                 185                 190         


Pro Pro Lys Asp His Arg Ile Leu Pro Gly Ile Thr Tyr Asp Val Val 
        195                 200                 205             


Leu Glu Leu Ala Glu Thr His Gly Val Pro His Ala Thr Arg Glu Ile 
    210                 215                 220                 


Ser Glu Leu Glu Leu Arg Thr Ala Arg Glu Ile Met Leu Thr Ser Ser 
225                 230                 235                 240 


Thr Lys Glu Ile Leu Pro Ile Thr Gln Leu Asp Gly Gln Pro Ile Gly 
                245                 250                 255     


Asn Gly Thr Pro Gly Pro Val Phe Gln Gln Leu Asp Arg Leu Tyr Gln 
            260                 265                 270         


Ala Tyr Lys Leu Glu Val Met Arg Gly His Ala Pro Arg Gln 
        275                 280                 285     


<210>  24
<211>  1293
<212>  DNA
<213>  Neisseria gonorrhoeae


<220>
<221>  CDS
<222>  (1)..(1293)

<400>  24
atg agg ata aat atg aac cgt aac gaa att tta ttc gac cgc gcc aag       48
Met Arg Ile Asn Met Asn Arg Asn Glu Ile Leu Phe Asp Arg Ala Lys         
1               5                   10                  15              

gcc atc atc ccc ggc ggc gtg aat tcg ccc gtg cgc gca ttc ggc agc       96
Ala Ile Ile Pro Gly Gly Val Asn Ser Pro Val Arg Ala Phe Gly Ser         
            20                  25                  30                  

gtc ggc ggc gtg ccg cgc ttc atc aaa aaa gcc gaa ggc gcg tat gtt      144
Val Gly Gly Val Pro Arg Phe Ile Lys Lys Ala Glu Gly Ala Tyr Val         
        35                  40                  45                      

tgg gac gaa aac ggc acg cgc tac acc gat tat gtc ggc tct tgg ggg      192
Trp Asp Glu Asn Gly Thr Arg Tyr Thr Asp Tyr Val Gly Ser Trp Gly         
    50                  55                  60                          

cct gcg att gtc gga cac gcg cat ccc gaa gtc gtc gaa gcc gtg cgc      240
Pro Ala Ile Val Gly His Ala His Pro Glu Val Val Glu Ala Val Arg         
65                  70                  75                  80          

gaa gct gcg ttg ggc ggt ttg tcg ttc ggc gcg ccc acc gaa ggc gaa      288
Glu Ala Ala Leu Gly Gly Leu Ser Phe Gly Ala Pro Thr Glu Gly Glu         
                85                  90                  95              

atc gcc att gcc gaa caa att gcc gaa att atg ccg tct gtc gaa cgg      336
Ile Ala Ile Ala Glu Gln Ile Ala Glu Ile Met Pro Ser Val Glu Arg         
            100                 105                 110                 

ctg cgc ctc gtc agc tcc ggc acg gaa gcg acg atg act gcc atc cgt      384
Leu Arg Leu Val Ser Ser Gly Thr Glu Ala Thr Met Thr Ala Ile Arg         
        115                 120                 125                     

ctg gca cgc ggt ttt acc ggc cgc gac aaa atc atc aaa ttt gaa ggc      432
Leu Ala Arg Gly Phe Thr Gly Arg Asp Lys Ile Ile Lys Phe Glu Gly         
    130                 135                 140                         

tgc tac cac ggc cat tcc gac agc ctg ttg gtg aaa gca ggc agc ggt      480
Cys Tyr His Gly His Ser Asp Ser Leu Leu Val Lys Ala Gly Ser Gly         
145                 150                 155                 160         

ctg ctt acc ttc ggc aat cct tct tcc gcc ggt gtg cct gcc gac ttt      528
Leu Leu Thr Phe Gly Asn Pro Ser Ser Ala Gly Val Pro Ala Asp Phe         
                165                 170                 175             

acc aaa cat act ttg gta ctc gaa tac aac aac atc gcc caa ctc gaa      576
Thr Lys His Thr Leu Val Leu Glu Tyr Asn Asn Ile Ala Gln Leu Glu         
            180                 185                 190                 

gaa gcc ttt gcc caa agc ggc gac gaa atc gcc tgc gtg att gtc gaa      624
Glu Ala Phe Ala Gln Ser Gly Asp Glu Ile Ala Cys Val Ile Val Glu         
        195                 200                 205                     

ccc ttc gtc ggc aat atg aac ctc gtc cgc ccg acc gaa gcc ttt gtc      672
Pro Phe Val Gly Asn Met Asn Leu Val Arg Pro Thr Glu Ala Phe Val         
    210                 215                 220                         

aaa gcc ttg cgc gga ttg acc gaa aaa cac ggc gcg gtg ttg att tac      720
Lys Ala Leu Arg Gly Leu Thr Glu Lys His Gly Ala Val Leu Ile Tyr         
225                 230                 235                 240         

gac gaa gtg atg acc ggt ttc cgc gtc gcg ctc ggc ggc gcg cag tcg      768
Asp Glu Val Met Thr Gly Phe Arg Val Ala Leu Gly Gly Ala Gln Ser         
                245                 250                 255             

ctg cac ggc atc acg ccc gac ctg acc acg atg ggc aaa gtc atc ggc      816
Leu His Gly Ile Thr Pro Asp Leu Thr Thr Met Gly Lys Val Ile Gly         
            260                 265                 270                 

ggc ggt atg ccg ctt gcc gcg ttc ggc gga cgc aaa gac atc atg gaa      864
Gly Gly Met Pro Leu Ala Ala Phe Gly Gly Arg Lys Asp Ile Met Glu         
        275                 280                 285                     

tgt att tcc ccg ttg ggc ggc gtg tat cag gca ggt aca tta tca ggc      912
Cys Ile Ser Pro Leu Gly Gly Val Tyr Gln Ala Gly Thr Leu Ser Gly         
    290                 295                 300                         

aac ccg att gcc gtc gcc gcc ggc ttg aaa acg ctg gaa atc atc cag      960
Asn Pro Ile Ala Val Ala Ala Gly Leu Lys Thr Leu Glu Ile Ile Gln         
305                 310                 315                 320         

cgc gaa ggc ttc tat gaa aac ctg acc gcc ttg aca caa cgc ctt gcc     1008
Arg Glu Gly Phe Tyr Glu Asn Leu Thr Ala Leu Thr Gln Arg Leu Ala         
                325                 330                 335             

aac ggt att gcc gcc gcc aaa gcg cac ggt atc gag ttt gcc gcc gac     1056
Asn Gly Ile Ala Ala Ala Lys Ala His Gly Ile Glu Phe Ala Ala Asp         
            340                 345                 350                 

agc gtg ggc ggt atg ttc ggt ctg tat ttc gcc gca cac gtg ccg cga     1104
Ser Val Gly Gly Met Phe Gly Leu Tyr Phe Ala Ala His Val Pro Arg         
        355                 360                 365                     

aac tat gcc gat atg gcg cgc tcc aat atc gac gct ttc aaa cgc ttc     1152
Asn Tyr Ala Asp Met Ala Arg Ser Asn Ile Asp Ala Phe Lys Arg Phe         
    370                 375                 380                         

ttc cac ggc atg ctc gac cgc ggc att gcc ttc ggc ccg tcc gct tat     1200
Phe His Gly Met Leu Asp Arg Gly Ile Ala Phe Gly Pro Ser Ala Tyr         
385                 390                 395                 400         

gaa gcg ggt ttc gtt tcc gcc gcg cat acg ccc gag ctg att gac gaa     1248
Glu Ala Gly Phe Val Ser Ala Ala His Thr Pro Glu Leu Ile Asp Glu         
                405                 410                 415             

acg gtt gcg gtt gcg gtt gaa gtg ttc aag gcg atg gct gca tga         1293
Thr Val Ala Val Ala Val Glu Val Phe Lys Ala Met Ala Ala                 
            420                 425                 430                 


<210>  25
<211>  430
<212>  PRT
<213>  Neisseria gonorrhoeae

<400>  25

Met Arg Ile Asn Met Asn Arg Asn Glu Ile Leu Phe Asp Arg Ala Lys 
1               5                   10                  15      


Ala Ile Ile Pro Gly Gly Val Asn Ser Pro Val Arg Ala Phe Gly Ser 
            20                  25                  30          


Val Gly Gly Val Pro Arg Phe Ile Lys Lys Ala Glu Gly Ala Tyr Val 
        35                  40                  45              


Trp Asp Glu Asn Gly Thr Arg Tyr Thr Asp Tyr Val Gly Ser Trp Gly 
    50                  55                  60                  


Pro Ala Ile Val Gly His Ala His Pro Glu Val Val Glu Ala Val Arg 
65                  70                  75                  80  


Glu Ala Ala Leu Gly Gly Leu Ser Phe Gly Ala Pro Thr Glu Gly Glu 
                85                  90                  95      


Ile Ala Ile Ala Glu Gln Ile Ala Glu Ile Met Pro Ser Val Glu Arg 
            100                 105                 110         


Leu Arg Leu Val Ser Ser Gly Thr Glu Ala Thr Met Thr Ala Ile Arg 
        115                 120                 125             


Leu Ala Arg Gly Phe Thr Gly Arg Asp Lys Ile Ile Lys Phe Glu Gly 
    130                 135                 140                 


Cys Tyr His Gly His Ser Asp Ser Leu Leu Val Lys Ala Gly Ser Gly 
145                 150                 155                 160 


Leu Leu Thr Phe Gly Asn Pro Ser Ser Ala Gly Val Pro Ala Asp Phe 
                165                 170                 175     


Thr Lys His Thr Leu Val Leu Glu Tyr Asn Asn Ile Ala Gln Leu Glu 
            180                 185                 190         


Glu Ala Phe Ala Gln Ser Gly Asp Glu Ile Ala Cys Val Ile Val Glu 
        195                 200                 205             


Pro Phe Val Gly Asn Met Asn Leu Val Arg Pro Thr Glu Ala Phe Val 
    210                 215                 220                 


Lys Ala Leu Arg Gly Leu Thr Glu Lys His Gly Ala Val Leu Ile Tyr 
225                 230                 235                 240 


Asp Glu Val Met Thr Gly Phe Arg Val Ala Leu Gly Gly Ala Gln Ser 
                245                 250                 255     


Leu His Gly Ile Thr Pro Asp Leu Thr Thr Met Gly Lys Val Ile Gly 
            260                 265                 270         


Gly Gly Met Pro Leu Ala Ala Phe Gly Gly Arg Lys Asp Ile Met Glu 
        275                 280                 285             


Cys Ile Ser Pro Leu Gly Gly Val Tyr Gln Ala Gly Thr Leu Ser Gly 
    290                 295                 300                 


Asn Pro Ile Ala Val Ala Ala Gly Leu Lys Thr Leu Glu Ile Ile Gln 
305                 310                 315                 320 


Arg Glu Gly Phe Tyr Glu Asn Leu Thr Ala Leu Thr Gln Arg Leu Ala 
                325                 330                 335     


Asn Gly Ile Ala Ala Ala Lys Ala His Gly Ile Glu Phe Ala Ala Asp 
            340                 345                 350         


Ser Val Gly Gly Met Phe Gly Leu Tyr Phe Ala Ala His Val Pro Arg 
        355                 360                 365             


Asn Tyr Ala Asp Met Ala Arg Ser Asn Ile Asp Ala Phe Lys Arg Phe 
    370                 375                 380                 


Phe His Gly Met Leu Asp Arg Gly Ile Ala Phe Gly Pro Ser Ala Tyr 
385                 390                 395                 400 


Glu Ala Gly Phe Val Ser Ala Ala His Thr Pro Glu Leu Ile Asp Glu 
                405                 410                 415     


Thr Val Ala Val Ala Val Glu Val Phe Lys Ala Met Ala Ala 
            420                 425                 430 


<210>  26
<211>  924
<212>  DNA
<213>  Pseudomonas aeruginosa (gi 9951299)


<220>
<221>  CDS
<222>  (1)..(924)

<400>  26
atg tcg atg gcc gat cgt gat ggc gtg atc tgg tat gac ggt gaa ctg       48
Met Ser Met Ala Asp Arg Asp Gly Val Ile Trp Tyr Asp Gly Glu Leu         
1               5                   10                  15              

gtg cag tgg cgc gac gcg acc acg cac gtg ctg acc cat acc ctg cac       96
Val Gln Trp Arg Asp Ala Thr Thr His Val Leu Thr His Thr Leu His         
            20                  25                  30                  

tat gga atg ggc gtg ttc gag ggc gtg cgc gcc tac gac acc ccg cag      144
Tyr Gly Met Gly Val Phe Glu Gly Val Arg Ala Tyr Asp Thr Pro Gln         
        35                  40                  45                      

ggc acg gcg atc ttc cgc ctg cag gcg cat acc gac cgg ctg ttc gac      192
Gly Thr Ala Ile Phe Arg Leu Gln Ala His Thr Asp Arg Leu Phe Asp         
    50                  55                  60                          

tcc gcg cac atc atg aac atg cag atc ccg tac agc cgc gac gag atc      240
Ser Ala His Ile Met Asn Met Gln Ile Pro Tyr Ser Arg Asp Glu Ile         
65                  70                  75                  80          

aac gag gcg acc cgc gcc gcc gtg cgc gag aac aac ctg gaa agc gcc      288
Asn Glu Ala Thr Arg Ala Ala Val Arg Glu Asn Asn Leu Glu Ser Ala         
                85                  90                  95              

tat atc cgc ccg atg gtg ttc tac gga agc gaa ggc atg ggc ctg cgc      336
Tyr Ile Arg Pro Met Val Phe Tyr Gly Ser Glu Gly Met Gly Leu Arg         
            100                 105                 110                 

gcc agc ggc ctg aag gtc cat gtg atc atc gcc gcc tgg agc tgg ggc      384
Ala Ser Gly Leu Lys Val His Val Ile Ile Ala Ala Trp Ser Trp Gly         
        115                 120                 125                     

gcc tac atg ggc gag gaa gcc ctg cag caa ggc atc aag gtg cgc acc      432
Ala Tyr Met Gly Glu Glu Ala Leu Gln Gln Gly Ile Lys Val Arg Thr         
    130                 135                 140                         

agt tcc ttc acc cgc cac cac gtc aac atc tcg atg acc cgc gcc aag      480
Ser Ser Phe Thr Arg His His Val Asn Ile Ser Met Thr Arg Ala Lys         
145                 150                 155                 160         

tcc aac ggc gcc tac atc aac tcg atg ctg gcc ctc cag gaa gcg atc      528
Ser Asn Gly Ala Tyr Ile Asn Ser Met Leu Ala Leu Gln Glu Ala Ile         
                165                 170                 175             

tcc ggc ggc gcc gac gag gcc atg atg ctc gat ccg gaa ggc tac gtg      576
Ser Gly Gly Ala Asp Glu Ala Met Met Leu Asp Pro Glu Gly Tyr Val         
            180                 185                 190                 

gcc gaa ggc tcc ggc gag aac atc ttc atc atc aag gat ggc gtg atc      624
Ala Glu Gly Ser Gly Glu Asn Ile Phe Ile Ile Lys Asp Gly Val Ile         
        195                 200                 205                     

tac acc ccg gaa gtc acc gcc tgc ctg aac ggc atc act cgt aac act      672
Tyr Thr Pro Glu Val Thr Ala Cys Leu Asn Gly Ile Thr Arg Asn Thr         
    210                 215                 220                         

atc ctg acc ctg gcc gcc gaa cac ggt ttt aaa ctg gtc gag aag cgc      720
Ile Leu Thr Leu Ala Ala Glu His Gly Phe Lys Leu Val Glu Lys Arg         
225                 230                 235                 240         

atc acc cgc gac gag gtg tac atc gcc gac gag gcc ttc ttc act ggc      768
Ile Thr Arg Asp Glu Val Tyr Ile Ala Asp Glu Ala Phe Phe Thr Gly         
                245                 250                 255             

act gcc gcg gaa gtc acg ccg atc cgc gaa gtg gac ggt cgc aag atc      816
Thr Ala Ala Glu Val Thr Pro Ile Arg Glu Val Asp Gly Arg Lys Ile         
            260                 265                 270                 

ggc gcc ggc cgc cgt ggc ccg gtc acc gaa aag ctg cag aaa gcc tat      864
Gly Ala Gly Arg Arg Gly Pro Val Thr Glu Lys Leu Gln Lys Ala Tyr         
        275                 280                 285                     

ttc gac ctg gtc agc ggc aag acc gag gcc cac gcc gag tgg cgt acc      912
Phe Asp Leu Val Ser Gly Lys Thr Glu Ala His Ala Glu Trp Arg Thr         
    290                 295                 300                         

ctg gtc aag taa                                                      924
Leu Val Lys                                                             
305                                                                     


<210>  27
<211>  307
<212>  PRT
<213>  Pseudomonas aeruginosa (gi 9951299)

<400>  27

Met Ser Met Ala Asp Arg Asp Gly Val Ile Trp Tyr Asp Gly Glu Leu 
1               5                   10                  15      


Val Gln Trp Arg Asp Ala Thr Thr His Val Leu Thr His Thr Leu His 
            20                  25                  30          


Tyr Gly Met Gly Val Phe Glu Gly Val Arg Ala Tyr Asp Thr Pro Gln 
        35                  40                  45              


Gly Thr Ala Ile Phe Arg Leu Gln Ala His Thr Asp Arg Leu Phe Asp 
    50                  55                  60                  


Ser Ala His Ile Met Asn Met Gln Ile Pro Tyr Ser Arg Asp Glu Ile 
65                  70                  75                  80  


Asn Glu Ala Thr Arg Ala Ala Val Arg Glu Asn Asn Leu Glu Ser Ala 
                85                  90                  95      


Tyr Ile Arg Pro Met Val Phe Tyr Gly Ser Glu Gly Met Gly Leu Arg 
            100                 105                 110         


Ala Ser Gly Leu Lys Val His Val Ile Ile Ala Ala Trp Ser Trp Gly 
        115                 120                 125             


Ala Tyr Met Gly Glu Glu Ala Leu Gln Gln Gly Ile Lys Val Arg Thr 
    130                 135                 140                 


Ser Ser Phe Thr Arg His His Val Asn Ile Ser Met Thr Arg Ala Lys 
145                 150                 155                 160 


Ser Asn Gly Ala Tyr Ile Asn Ser Met Leu Ala Leu Gln Glu Ala Ile 
                165                 170                 175     


Ser Gly Gly Ala Asp Glu Ala Met Met Leu Asp Pro Glu Gly Tyr Val 
            180                 185                 190         


Ala Glu Gly Ser Gly Glu Asn Ile Phe Ile Ile Lys Asp Gly Val Ile 
        195                 200                 205             


Tyr Thr Pro Glu Val Thr Ala Cys Leu Asn Gly Ile Thr Arg Asn Thr 
    210                 215                 220                 


Ile Leu Thr Leu Ala Ala Glu His Gly Phe Lys Leu Val Glu Lys Arg 
225                 230                 235                 240 


Ile Thr Arg Asp Glu Val Tyr Ile Ala Asp Glu Ala Phe Phe Thr Gly 
                245                 250                 255     


Thr Ala Ala Glu Val Thr Pro Ile Arg Glu Val Asp Gly Arg Lys Ile 
            260                 265                 270         


Gly Ala Gly Arg Arg Gly Pro Val Thr Glu Lys Leu Gln Lys Ala Tyr 
        275                 280                 285             


Phe Asp Leu Val Ser Gly Lys Thr Glu Ala His Ala Glu Trp Arg Thr 
    290                 295                 300                 


Leu Val Lys 
305         


<210>  28
<211>  1407
<212>  DNA
<213>  Rhodopseudomonas palustris


<220>
<221>  CDS
<222>  (1)..(1407)

<400>  28
atg aag ctg ata ccg tgc cgc gcc ttt cac ccc ccg gcc gcg cag tgc       48
Met Lys Leu Ile Pro Cys Arg Ala Phe His Pro Pro Ala Ala Gln Cys         
1               5                   10                  15              

atg agg agc gcc atg tta gac aag atc aag ccc acg tcc gcc gtc aac       96
Met Arg Ser Ala Met Leu Asp Lys Ile Lys Pro Thr Ser Ala Val Asn         
            20                  25                  30                  

gcg ccg aac gat ctc aac gcg ttc tgg atg ccg ttc acc gcg aac cgg      144
Ala Pro Asn Asp Leu Asn Ala Phe Trp Met Pro Phe Thr Ala Asn Arg         
        35                  40                  45                      

gcc ttc aag cgc gcg ccg aag atg gtc gtg ggt gcc gaa ggc atg cac      192
Ala Phe Lys Arg Ala Pro Lys Met Val Val Gly Ala Glu Gly Met His         
    50                  55                  60                          

tac atc acc gcc gat ggt cgc aag atc atc gac gcc gcc tcg ggc atg      240
Tyr Ile Thr Ala Asp Gly Arg Lys Ile Ile Asp Ala Ala Ser Gly Met         
65                  70                  75                  80          

tgg tgc acc aat gcg ggc cat ggc cgc aag gaa atc gcc gag gcg atc      288
Trp Cys Thr Asn Ala Gly His Gly Arg Lys Glu Ile Ala Glu Ala Ile         
                85                  90                  95              

aag gcg cag gcc gat gaa ctc gac ttc tcg ccg ccg ttc cag ttc ggc      336
Lys Ala Gln Ala Asp Glu Leu Asp Phe Ser Pro Pro Phe Gln Phe Gly         
            100                 105                 110                 

cag ccg aag gcg ttc gaa ctc gcc agc cgg atc gcc gat ctg gcg ccg      384
Gln Pro Lys Ala Phe Glu Leu Ala Ser Arg Ile Ala Asp Leu Ala Pro         
        115                 120                 125                     

gaa ggc ctc gat cac gtg ttc ttc tgc aat tcg ggc tcg gaa gcc ggc      432
Glu Gly Leu Asp His Val Phe Phe Cys Asn Ser Gly Ser Glu Ala Gly         
    130                 135                 140                         

gac acc gcg ctg aag atc gcg gtc gcc tat cag cag atc aag ggc cag      480
Asp Thr Ala Leu Lys Ile Ala Val Ala Tyr Gln Gln Ile Lys Gly Gln         
145                 150                 155                 160         

ggc tca cgc acc cgc ctg atc ggc cgc gag cgc ggc tat cac ggc gtc      528
Gly Ser Arg Thr Arg Leu Ile Gly Arg Glu Arg Gly Tyr His Gly Val         
                165                 170                 175             

ggc ttc ggc ggc acc gcg gtc ggc ggc atc ggc aac aac cgc aag atg      576
Gly Phe Gly Gly Thr Ala Val Gly Gly Ile Gly Asn Asn Arg Lys Met         
            180                 185                 190                 

ttc ggt ccg ctg ctc aac ggc gtc gat cat ctg cct gcg act tat gat      624
Phe Gly Pro Leu Leu Asn Gly Val Asp His Leu Pro Ala Thr Tyr Asp         
        195                 200                 205                     

cgc gac aag cag gct ttc acc atc ggc gag ccg gaa tac ggc gcg cac      672
Arg Asp Lys Gln Ala Phe Thr Ile Gly Glu Pro Glu Tyr Gly Ala His         
    210                 215                 220                         

ttc gcc gaa gcg ctt gaa ggc ctc gtc aat ctg cac ggc gcc aac acc      720
Phe Ala Glu Ala Leu Glu Gly Leu Val Asn Leu His Gly Ala Asn Thr         
225                 230                 235                 240         

atc gcg gcg gtg atc gtc gag ccg atg gcc ggc tcc acc ggc gtg ctg      768
Ile Ala Ala Val Ile Val Glu Pro Met Ala Gly Ser Thr Gly Val Leu         
                245                 250                 255             

ccg gcg ccg aag ggc tat ctc aag aag ctg cgc gag atc acc aag aag      816
Pro Ala Pro Lys Gly Tyr Leu Lys Lys Leu Arg Glu Ile Thr Lys Lys         
            260                 265                 270                 

cac ggc atc ctg ctg atc ttc gac gag gtc atc acc ggc tac ggc cgt      864
His Gly Ile Leu Leu Ile Phe Asp Glu Val Ile Thr Gly Tyr Gly Arg         
        275                 280                 285                     

ctc ggc tat gcc ttc gcg tcc gaa cgt tac ggc gtc acc ccg gac atg      912
Leu Gly Tyr Ala Phe Ala Ser Glu Arg Tyr Gly Val Thr Pro Asp Met         
    290                 295                 300                         

atc acc ttc gcc aag ggc gtc acc aat ggt gcg gtg ccg atg ggc ggc      960
Ile Thr Phe Ala Lys Gly Val Thr Asn Gly Ala Val Pro Met Gly Gly         
305                 310                 315                 320         

gtg atc acc tcg gcg gag atc cac gat gcg ttc atg acc ggc ccc gag     1008
Val Ile Thr Ser Ala Glu Ile His Asp Ala Phe Met Thr Gly Pro Glu         
                325                 330                 335             

cac gcg gtc gag ctg gcg cac ggc tac acc tat tcg gcg cat ccg ctc     1056
His Ala Val Glu Leu Ala His Gly Tyr Thr Tyr Ser Ala His Pro Leu         
            340                 345                 350                 

gcc tgc gcg gcc ggc atc gcc acc ctc gac atc tac cgc gac gag aag     1104
Ala Cys Ala Ala Gly Ile Ala Thr Leu Asp Ile Tyr Arg Asp Glu Lys         
        355                 360                 365                     

ctg ttc gag cgc gcc aag gcg ctg gag ccg aag ttt gcc gag gcg gtg     1152
Leu Phe Glu Arg Ala Lys Ala Leu Glu Pro Lys Phe Ala Glu Ala Val         
    370                 375                 380                         

atg tcg ctg aag tcg gcc ccg aac gtg gtc gac atc cgc acc gtc ggc     1200
Met Ser Leu Lys Ser Ala Pro Asn Val Val Asp Ile Arg Thr Val Gly         
385                 390                 395                 400         

ctg acg gcg ggt atc gac ctc gct tcg atc gcc gat gcg gtc ggc aag     1248
Leu Thr Ala Gly Ile Asp Leu Ala Ser Ile Ala Asp Ala Val Gly Lys         
                405                 410                 415             

cgt ggc ttc gaa gcg atg aat gcc ggc ttc cac gac cac gag ctg atg     1296
Arg Gly Phe Glu Ala Met Asn Ala Gly Phe His Asp His Glu Leu Met         
            420                 425                 430                 

ctg cgg atc gcc ggc gac acc ctg gcg ctg acc ccg ccg ctg atc ctc     1344
Leu Arg Ile Ala Gly Asp Thr Leu Ala Leu Thr Pro Pro Leu Ile Leu         
        435                 440                 445                     

agc gag gac cac atc ggt gag atc gtc gac aag gtc ggc aag gtg atc     1392
Ser Glu Asp His Ile Gly Glu Ile Val Asp Lys Val Gly Lys Val Ile         
    450                 455                 460                         

cgc gcg gtc gcc tga                                                 1407
Arg Ala Val Ala                                                         
465                                                                     


<210>  29
<211>  468
<212>  PRT
<213>  Rhodopseudomonas palustris

<400>  29

Met Lys Leu Ile Pro Cys Arg Ala Phe His Pro Pro Ala Ala Gln Cys 
1               5                   10                  15      


Met Arg Ser Ala Met Leu Asp Lys Ile Lys Pro Thr Ser Ala Val Asn 
            20                  25                  30          


Ala Pro Asn Asp Leu Asn Ala Phe Trp Met Pro Phe Thr Ala Asn Arg 
        35                  40                  45              


Ala Phe Lys Arg Ala Pro Lys Met Val Val Gly Ala Glu Gly Met His 
    50                  55                  60                  


Tyr Ile Thr Ala Asp Gly Arg Lys Ile Ile Asp Ala Ala Ser Gly Met 
65                  70                  75                  80  


Trp Cys Thr Asn Ala Gly His Gly Arg Lys Glu Ile Ala Glu Ala Ile 
                85                  90                  95      


Lys Ala Gln Ala Asp Glu Leu Asp Phe Ser Pro Pro Phe Gln Phe Gly 
            100                 105                 110         


Gln Pro Lys Ala Phe Glu Leu Ala Ser Arg Ile Ala Asp Leu Ala Pro 
        115                 120                 125             


Glu Gly Leu Asp His Val Phe Phe Cys Asn Ser Gly Ser Glu Ala Gly 
    130                 135                 140                 


Asp Thr Ala Leu Lys Ile Ala Val Ala Tyr Gln Gln Ile Lys Gly Gln 
145                 150                 155                 160 


Gly Ser Arg Thr Arg Leu Ile Gly Arg Glu Arg Gly Tyr His Gly Val 
                165                 170                 175     


Gly Phe Gly Gly Thr Ala Val Gly Gly Ile Gly Asn Asn Arg Lys Met 
            180                 185                 190         


Phe Gly Pro Leu Leu Asn Gly Val Asp His Leu Pro Ala Thr Tyr Asp 
        195                 200                 205             


Arg Asp Lys Gln Ala Phe Thr Ile Gly Glu Pro Glu Tyr Gly Ala His 
    210                 215                 220                 


Phe Ala Glu Ala Leu Glu Gly Leu Val Asn Leu His Gly Ala Asn Thr 
225                 230                 235                 240 


Ile Ala Ala Val Ile Val Glu Pro Met Ala Gly Ser Thr Gly Val Leu 
                245                 250                 255     


Pro Ala Pro Lys Gly Tyr Leu Lys Lys Leu Arg Glu Ile Thr Lys Lys 
            260                 265                 270         


His Gly Ile Leu Leu Ile Phe Asp Glu Val Ile Thr Gly Tyr Gly Arg 
        275                 280                 285             


Leu Gly Tyr Ala Phe Ala Ser Glu Arg Tyr Gly Val Thr Pro Asp Met 
    290                 295                 300                 


Ile Thr Phe Ala Lys Gly Val Thr Asn Gly Ala Val Pro Met Gly Gly 
305                 310                 315                 320 


Val Ile Thr Ser Ala Glu Ile His Asp Ala Phe Met Thr Gly Pro Glu 
                325                 330                 335     


His Ala Val Glu Leu Ala His Gly Tyr Thr Tyr Ser Ala His Pro Leu 
            340                 345                 350         


Ala Cys Ala Ala Gly Ile Ala Thr Leu Asp Ile Tyr Arg Asp Glu Lys 
        355                 360                 365             


Leu Phe Glu Arg Ala Lys Ala Leu Glu Pro Lys Phe Ala Glu Ala Val 
    370                 375                 380                 


Met Ser Leu Lys Ser Ala Pro Asn Val Val Asp Ile Arg Thr Val Gly 
385                 390                 395                 400 


Leu Thr Ala Gly Ile Asp Leu Ala Ser Ile Ala Asp Ala Val Gly Lys 
                405                 410                 415     


Arg Gly Phe Glu Ala Met Asn Ala Gly Phe His Asp His Glu Leu Met 
            420                 425                 430         


Leu Arg Ile Ala Gly Asp Thr Leu Ala Leu Thr Pro Pro Leu Ile Leu 
        435                 440                 445             


Ser Glu Asp His Ile Gly Glu Ile Val Asp Lys Val Gly Lys Val Ile 
    450                 455                 460                 


Arg Ala Val Ala 
465             


<210>  30
<211>  1263
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1263)

<400>  30
atg cca cat tca ctg ttc agc acc gat acc gat ctc acc gcc gaa aat       48
Met Pro His Ser Leu Phe Ser Thr Asp Thr Asp Leu Thr Ala Glu Asn         
1               5                   10                  15              

ctg ctg cgt ttg ccc gct gaa ttt ggc tgc ccg gtg tgg gtc tac gat       96
Leu Leu Arg Leu Pro Ala Glu Phe Gly Cys Pro Val Trp Val Tyr Asp         
            20                  25                  30                  

gcg caa att att cgt cgg cag att gca gcg ctg aaa cag ttt gat gtg      144
Ala Gln Ile Ile Arg Arg Gln Ile Ala Ala Leu Lys Gln Phe Asp Val         
        35                  40                  45                      

gtg cgc ttt gca cag aaa gcc tgt tcc aat att cat att ttg cgc tta      192
Val Arg Phe Ala Gln Lys Ala Cys Ser Asn Ile His Ile Leu Arg Leu         
    50                  55                  60                          

atg cgt gag cag ggc gtg aaa gtg gat tcc gtc tcg tta ggc gaa ata      240
Met Arg Glu Gln Gly Val Lys Val Asp Ser Val Ser Leu Gly Glu Ile         
65                  70                  75                  80          

gag cgt gcg ttg gcg gcg ggt tac aat ccg caa acg cac ccc gat gat      288
Glu Arg Ala Leu Ala Ala Gly Tyr Asn Pro Gln Thr His Pro Asp Asp         
                85                  90                  95              

att gtt ttt acg gca gat gtt atc gat cag gcg acg ctt gaa cgc gtc      336
Ile Val Phe Thr Ala Asp Val Ile Asp Gln Ala Thr Leu Glu Arg Val         
            100                 105                 110                 

agt gaa ttg caa att ccg gtg aat gcg ggt tct gtt gat atg ctc gac      384
Ser Glu Leu Gln Ile Pro Val Asn Ala Gly Ser Val Asp Met Leu Asp         
        115                 120                 125                     

caa ctg ggc cag gtt tcg cca ggg cat cgg gta tgg ctg cgc gtt aat      432
Gln Leu Gly Gln Val Ser Pro Gly His Arg Val Trp Leu Arg Val Asn         
    130                 135                 140                         

ccg ggg ttt ggt cac gga cat agc caa aaa acc aat acc ggt ggc gaa      480
Pro Gly Phe Gly His Gly His Ser Gln Lys Thr Asn Thr Gly Gly Glu         
145                 150                 155                 160         

aac agc aag cac ggt atc tgg tac acc gat ctg ccc gcc gca ctg gac      528
Asn Ser Lys His Gly Ile Trp Tyr Thr Asp Leu Pro Ala Ala Leu Asp         
                165                 170                 175             

gtg ata caa cgt cat cat ctg cag ctg gtc ggc att cac atg cac att      576
Val Ile Gln Arg His His Leu Gln Leu Val Gly Ile His Met His Ile         
            180                 185                 190                 

ggt tct ggc gtt gat tat gcc cat ctg gaa cag gtg tgt ggt gct atg      624
Gly Ser Gly Val Asp Tyr Ala His Leu Glu Gln Val Cys Gly Ala Met         
        195                 200                 205                     

gtg cgt cag gtc atc gaa ttc ggt cag gat tta cag gct att tct gcg      672
Val Arg Gln Val Ile Glu Phe Gly Gln Asp Leu Gln Ala Ile Ser Ala         
    210                 215                 220                         

ggc ggt ggg ctt tct gtt cct tat caa cag ggt gaa gag gcg gtt gat      720
Gly Gly Gly Leu Ser Val Pro Tyr Gln Gln Gly Glu Glu Ala Val Asp         
225                 230                 235                 240         

acc gaa cat tat tat ggt ctg tgg aat gcc gcg cgt gag caa atc gcc      768
Thr Glu His Tyr Tyr Gly Leu Trp Asn Ala Ala Arg Glu Gln Ile Ala         
                245                 250                 255             

cgc cat ttg ggc cac cct gtg aaa ctg gaa att gaa ccg ggt cgc ttc      816
Arg His Leu Gly His Pro Val Lys Leu Glu Ile Glu Pro Gly Arg Phe         
            260                 265                 270                 

ctg gta gcg cag tct ggc gta tta att act cag gtg cgg agc gtc aaa      864
Leu Val Ala Gln Ser Gly Val Leu Ile Thr Gln Val Arg Ser Val Lys         
        275                 280                 285                     

caa atg ggg agc cgc cac ttt gtg ctg gtt gat gcc ggg ttc aac gat      912
Gln Met Gly Ser Arg His Phe Val Leu Val Asp Ala Gly Phe Asn Asp         
    290                 295                 300                         

ctg atg cgc ccg gca atg tac ggt agt tac cac cat atc agt gcc ctg      960
Leu Met Arg Pro Ala Met Tyr Gly Ser Tyr His His Ile Ser Ala Leu         
305                 310                 315                 320         

gca gct gat ggt cgt tct ctg gaa cac gcg cca acg gtg gaa acc gtc     1008
Ala Ala Asp Gly Arg Ser Leu Glu His Ala Pro Thr Val Glu Thr Val         
                325                 330                 335             

gtc gcc gga ccg tta tgt gaa tcg ggc gat gtc ttt acc cag cag gaa     1056
Val Ala Gly Pro Leu Cys Glu Ser Gly Asp Val Phe Thr Gln Gln Glu         
            340                 345                 350                 

ggg gga aat gtt gaa acc cgc gcc ttg ccg gaa gtg aag gca ggt gat     1104
Gly Gly Asn Val Glu Thr Arg Ala Leu Pro Glu Val Lys Ala Gly Asp         
        355                 360                 365                     

tat ctg gta ctg cat gat aca ggg gca tat ggc gca tca atg tca tcc     1152
Tyr Leu Val Leu His Asp Thr Gly Ala Tyr Gly Ala Ser Met Ser Ser         
    370                 375                 380                         

aac tac aat agc cgt ccg ctg tta cca gaa gtt ctg ttt gat aat ggt     1200
Asn Tyr Asn Ser Arg Pro Leu Leu Pro Glu Val Leu Phe Asp Asn Gly         
385                 390                 395                 400         

cag gcg cgg ttg att cgc cgt cgc cag acc atc gaa gaa tta ctg gcg     1248
Gln Ala Arg Leu Ile Arg Arg Arg Gln Thr Ile Glu Glu Leu Leu Ala         
                405                 410                 415             

ctg gaa ttg ctt taa                                                 1263
Leu Glu Leu Leu                                                         
            420                                                         


<210>  31
<211>  420
<212>  PRT
<213>  Escherichia coli

<400>  31

Met Pro His Ser Leu Phe Ser Thr Asp Thr Asp Leu Thr Ala Glu Asn 
1               5                   10                  15      


Leu Leu Arg Leu Pro Ala Glu Phe Gly Cys Pro Val Trp Val Tyr Asp 
            20                  25                  30          


Ala Gln Ile Ile Arg Arg Gln Ile Ala Ala Leu Lys Gln Phe Asp Val 
        35                  40                  45              


Val Arg Phe Ala Gln Lys Ala Cys Ser Asn Ile His Ile Leu Arg Leu 
    50                  55                  60                  


Met Arg Glu Gln Gly Val Lys Val Asp Ser Val Ser Leu Gly Glu Ile 
65                  70                  75                  80  


Glu Arg Ala Leu Ala Ala Gly Tyr Asn Pro Gln Thr His Pro Asp Asp 
                85                  90                  95      


Ile Val Phe Thr Ala Asp Val Ile Asp Gln Ala Thr Leu Glu Arg Val 
            100                 105                 110         


Ser Glu Leu Gln Ile Pro Val Asn Ala Gly Ser Val Asp Met Leu Asp 
        115                 120                 125             


Gln Leu Gly Gln Val Ser Pro Gly His Arg Val Trp Leu Arg Val Asn 
    130                 135                 140                 


Pro Gly Phe Gly His Gly His Ser Gln Lys Thr Asn Thr Gly Gly Glu 
145                 150                 155                 160 


Asn Ser Lys His Gly Ile Trp Tyr Thr Asp Leu Pro Ala Ala Leu Asp 
                165                 170                 175     


Val Ile Gln Arg His His Leu Gln Leu Val Gly Ile His Met His Ile 
            180                 185                 190         


Gly Ser Gly Val Asp Tyr Ala His Leu Glu Gln Val Cys Gly Ala Met 
        195                 200                 205             


Val Arg Gln Val Ile Glu Phe Gly Gln Asp Leu Gln Ala Ile Ser Ala 
    210                 215                 220                 


Gly Gly Gly Leu Ser Val Pro Tyr Gln Gln Gly Glu Glu Ala Val Asp 
225                 230                 235                 240 


Thr Glu His Tyr Tyr Gly Leu Trp Asn Ala Ala Arg Glu Gln Ile Ala 
                245                 250                 255     


Arg His Leu Gly His Pro Val Lys Leu Glu Ile Glu Pro Gly Arg Phe 
            260                 265                 270         


Leu Val Ala Gln Ser Gly Val Leu Ile Thr Gln Val Arg Ser Val Lys 
        275                 280                 285             


Gln Met Gly Ser Arg His Phe Val Leu Val Asp Ala Gly Phe Asn Asp 
    290                 295                 300                 


Leu Met Arg Pro Ala Met Tyr Gly Ser Tyr His His Ile Ser Ala Leu 
305                 310                 315                 320 


Ala Ala Asp Gly Arg Ser Leu Glu His Ala Pro Thr Val Glu Thr Val 
                325                 330                 335     


Val Ala Gly Pro Leu Cys Glu Ser Gly Asp Val Phe Thr Gln Gln Glu 
            340                 345                 350         


Gly Gly Asn Val Glu Thr Arg Ala Leu Pro Glu Val Lys Ala Gly Asp 
        355                 360                 365             


Tyr Leu Val Leu His Asp Thr Gly Ala Tyr Gly Ala Ser Met Ser Ser 
    370                 375                 380                 


Asn Tyr Asn Ser Arg Pro Leu Leu Pro Glu Val Leu Phe Asp Asn Gly 
385                 390                 395                 400 


Gln Ala Arg Leu Ile Arg Arg Arg Gln Thr Ile Glu Glu Leu Leu Ala 
                405                 410                 415     


Leu Glu Leu Leu 
            420 


<210>  32
<211>  1265
<212>  DNA
<213>  Artificial

<220>
<223>  Escherichia.coli diaminopimelate decarboxylase LysA codon 
       optimised gene

<400>  32
atatgccaca ctctctgttt tctactgata ctgatctgac tgcggaaaac ctgctgcgtc     60

tgccggctga attcggttgt ccggtatggg tgtacgacgc tcagattatt cgtcgccaga    120

tcgcagcact gaagcagttc gatgtagtgc gttttgcaca gaaggcgtgc tccaacatcc    180

atatcctgcg cctgatgcgt gagcagggcg ttaaagttga ctccgtctct ctgggtgaga    240

ttgagcgcgc cctggcagcc ggctataacc cacagaccca tcctgacgac attgtattta    300

ctgccgacgt gatcgaccag gctactctgg aacgcgtttc tgaactgcag atcccggtta    360

atgctggttc tgtggacatg ctggaccagc tgggccaggt atccccaggt catcgtgtgt    420

ggctgcgtgt caacccaggt ttcggccacg gccactctca gaaaactaac actggtggtg    480

agaactccaa gcatggcatt tggtataccg atctgccggc tgcactggac gtaatccagc    540

gtcaccacct gcagctggtg ggcatccaca tgcacattgg ctccggcgta gactacgccc    600

acctggagca agtctgcggt gctatggtac gtcaggtaat cgagttcggc caagatctgc    660

aggcaatcag cgctggtggc ggcctgtctg taccttatca gcagggcgag gaggcggttg    720

acactgagca ctactacggt ctgtggaacg ccgctcgtga gcaaattgca cgtcacctgg    780

gccacccggt gaaactggag atcgagccgg gccgcttcct ggtagcacag tccggcgtac    840

tgattaccca ggtacgctct gttaaacaga tgggctcccg tcactttgtg ctggtagacg    900

caggcttcaa cgacctgatg cgtccggcta tgtatggttc ctatcatcac atctctgcgc    960

tggccgccga cggccgctct ctggaacacg cgccgacggt tgaaacggtg gtggctggtc   1020

cgctgtgcga gtccggcgac gttttcactc agcaggaggg cggcaatgta gagacgcgtg   1080

cgctgccgga agtgaaagcc ggtgattatc tggtgctgca tgataccggc gcctatggtg   1140

cgagcatgag cagcaactac aactctcgcc cgctgctgcc ggaggtcctg ttcgataacg   1200

gccaagcccg cctgatccgt cgtcgtcaga ccatcgagga actgctggca ctggagctgc   1260

tgtaa                                                               1265


<210>  33
<211>  1692
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(1692)

<400>  33
atg tct gaa att act ttg ggt aaa tat ttg ttc gaa aga tta aag caa       48
Met Ser Glu Ile Thr Leu Gly Lys Tyr Leu Phe Glu Arg Leu Lys Gln         
1               5                   10                  15              

gtc aac gtt aac acc gtt ttc ggt ttg cca ggt gac ttc aac ttg tcc       96
Val Asn Val Asn Thr Val Phe Gly Leu Pro Gly Asp Phe Asn Leu Ser         
            20                  25                  30                  

ttg ttg gac aag atc tac gaa gtt gaa ggt atg aga tgg gct ggt aac      144
Leu Leu Asp Lys Ile Tyr Glu Val Glu Gly Met Arg Trp Ala Gly Asn         
        35                  40                  45                      

gcc aac gaa ttg aac gct gct tac gcc gct gat ggt tac gct cgt atc      192
Ala Asn Glu Leu Asn Ala Ala Tyr Ala Ala Asp Gly Tyr Ala Arg Ile         
    50                  55                  60                          

aag ggt atg tct tgt atc atc acc acc ttc ggt gtc ggt gaa ttg tct      240
Lys Gly Met Ser Cys Ile Ile Thr Thr Phe Gly Val Gly Glu Leu Ser         
65                  70                  75                  80          

gct ttg aac ggt att gcc ggt tct tac gct gaa cac gtc ggt gtt ttg      288
Ala Leu Asn Gly Ile Ala Gly Ser Tyr Ala Glu His Val Gly Val Leu         
                85                  90                  95              

cac gtt gtt ggt gtc cca tcc atc tct gct caa gct aag caa ttg ttg      336
His Val Val Gly Val Pro Ser Ile Ser Ala Gln Ala Lys Gln Leu Leu         
            100                 105                 110                 

ttg cac cac acc ttg ggt aac ggt gac ttc act gtt ttc cac aga atg      384
Leu His His Thr Leu Gly Asn Gly Asp Phe Thr Val Phe His Arg Met         
        115                 120                 125                     

tct gcc aac att tct gaa acc act gct atg atc act gac att gct acc      432
Ser Ala Asn Ile Ser Glu Thr Thr Ala Met Ile Thr Asp Ile Ala Thr         
    130                 135                 140                         

gcc cca gct gaa att gac aga tgt atc aga acc act tac gtc acc caa      480
Ala Pro Ala Glu Ile Asp Arg Cys Ile Arg Thr Thr Tyr Val Thr Gln         
145                 150                 155                 160         

aga cca gtc tac tta ggt ttg cca gct aac ttg gtc gac ttg aac gtc      528
Arg Pro Val Tyr Leu Gly Leu Pro Ala Asn Leu Val Asp Leu Asn Val         
                165                 170                 175             

cca gct aag ttg ttg caa act cca att gac atg tct ttg aag cca aac      576
Pro Ala Lys Leu Leu Gln Thr Pro Ile Asp Met Ser Leu Lys Pro Asn         
            180                 185                 190                 

gat gct gaa tcc gaa aag gaa gtc att gac acc atc ttg gct ttg gtc      624
Asp Ala Glu Ser Glu Lys Glu Val Ile Asp Thr Ile Leu Ala Leu Val         
        195                 200                 205                     

aag gat gct aag aac cca gtt atc ttg gct gat gct tgt tgt tcc aga      672
Lys Asp Ala Lys Asn Pro Val Ile Leu Ala Asp Ala Cys Cys Ser Arg         
    210                 215                 220                         

cac gac gtc aag gct gaa act aag aag ttg att gac ttg act caa ttc      720
His Asp Val Lys Ala Glu Thr Lys Lys Leu Ile Asp Leu Thr Gln Phe         
225                 230                 235                 240         

cca gct ttc gtc acc cca atg ggt aag ggt tcc att gac gaa caa cac      768
Pro Ala Phe Val Thr Pro Met Gly Lys Gly Ser Ile Asp Glu Gln His         
                245                 250                 255             

cca aga tac ggt ggt gtt tac gtc ggt acc ttg tcc aag cca gaa gtt      816
Pro Arg Tyr Gly Gly Val Tyr Val Gly Thr Leu Ser Lys Pro Glu Val         
            260                 265                 270                 

aag gaa gcc gtt gaa tct gct gac ttg att ttg tct gtc ggt gct ttg      864
Lys Glu Ala Val Glu Ser Ala Asp Leu Ile Leu Ser Val Gly Ala Leu         
        275                 280                 285                     

ttg tct gat ttc aac acc ggt tct ttc tct tac tct tac aag acc aag      912
Leu Ser Asp Phe Asn Thr Gly Ser Phe Ser Tyr Ser Tyr Lys Thr Lys         
    290                 295                 300                         

aac att gtc gaa ttc cac tcc gac cac atg aag atc aga aac gcc act      960
Asn Ile Val Glu Phe His Ser Asp His Met Lys Ile Arg Asn Ala Thr         
305                 310                 315                 320         

ttc cca ggt gtc caa atg aaa ttc gtt ttg caa aag ttg ttg acc act     1008
Phe Pro Gly Val Gln Met Lys Phe Val Leu Gln Lys Leu Leu Thr Thr         
                325                 330                 335             

att gct gac gcc gct aag ggt tac aag cca gtt gct gtc cca gct aga     1056
Ile Ala Asp Ala Ala Lys Gly Tyr Lys Pro Val Ala Val Pro Ala Arg         
            340                 345                 350                 

act cca gct aac gct gct gtc cca gct tct acc cca ttg aag caa gaa     1104
Thr Pro Ala Asn Ala Ala Val Pro Ala Ser Thr Pro Leu Lys Gln Glu         
        355                 360                 365                     

tgg atg tgg aac caa ttg ggt aac ttc ttg caa gaa ggt gat gtt gtc     1152
Trp Met Trp Asn Gln Leu Gly Asn Phe Leu Gln Glu Gly Asp Val Val         
    370                 375                 380                         

att gct gaa acc ggt acc tcc gct ttc ggt atc aac caa acc act ttc     1200
Ile Ala Glu Thr Gly Thr Ser Ala Phe Gly Ile Asn Gln Thr Thr Phe         
385                 390                 395                 400         

cca aac aac acc tac ggt atc tct caa gtc tta tgg ggt tcc att ggt     1248
Pro Asn Asn Thr Tyr Gly Ile Ser Gln Val Leu Trp Gly Ser Ile Gly         
                405                 410                 415             

ttc acc act ggt gct acc ttg ggt gct gct ttc gct gct gaa gaa att     1296
Phe Thr Thr Gly Ala Thr Leu Gly Ala Ala Phe Ala Ala Glu Glu Ile         
            420                 425                 430                 

gat cca aag aag aga gtt atc tta ttc att ggt gac ggt tct ttg caa     1344
Asp Pro Lys Lys Arg Val Ile Leu Phe Ile Gly Asp Gly Ser Leu Gln         
        435                 440                 445                     

ttg act gtt caa gaa atc tcc acc atg atc aga tgg ggc ttg aag cca     1392
Leu Thr Val Gln Glu Ile Ser Thr Met Ile Arg Trp Gly Leu Lys Pro         
    450                 455                 460                         

tac ttg ttc gtc ttg aac aac gat ggt tac acc att gaa aag ttg att     1440
Tyr Leu Phe Val Leu Asn Asn Asp Gly Tyr Thr Ile Glu Lys Leu Ile         
465                 470                 475                 480         

cac ggt cca aag gct caa tac aac gaa att caa ggt tgg gac cac cta     1488
His Gly Pro Lys Ala Gln Tyr Asn Glu Ile Gln Gly Trp Asp His Leu         
                485                 490                 495             

tcc ttg ttg cca act ttc ggt gct aag gac tat gaa acc cac aga gtc     1536
Ser Leu Leu Pro Thr Phe Gly Ala Lys Asp Tyr Glu Thr His Arg Val         
            500                 505                 510                 

gct acc acc ggt gaa tgg gac aag ttg acc caa gac aag tct ttc aac     1584
Ala Thr Thr Gly Glu Trp Asp Lys Leu Thr Gln Asp Lys Ser Phe Asn         
        515                 520                 525                     

gac aac tct aag atc aga atg att gaa atc atg ttg cca gtc ttc gat     1632
Asp Asn Ser Lys Ile Arg Met Ile Glu Ile Met Leu Pro Val Phe Asp         
    530                 535                 540                         

gct cca caa aac ttg gtt gaa caa gct aag ttg act gct gct acc aac     1680
Ala Pro Gln Asn Leu Val Glu Gln Ala Lys Leu Thr Ala Ala Thr Asn         
545                 550                 555                 560         

gct aag caa taa                                                     1692
Ala Lys Gln                                                             
                                                                        


<210>  34
<211>  563
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  34

Met Ser Glu Ile Thr Leu Gly Lys Tyr Leu Phe Glu Arg Leu Lys Gln 
1               5                   10                  15      


Val Asn Val Asn Thr Val Phe Gly Leu Pro Gly Asp Phe Asn Leu Ser 
            20                  25                  30          


Leu Leu Asp Lys Ile Tyr Glu Val Glu Gly Met Arg Trp Ala Gly Asn 
        35                  40                  45              


Ala Asn Glu Leu Asn Ala Ala Tyr Ala Ala Asp Gly Tyr Ala Arg Ile 
    50                  55                  60                  


Lys Gly Met Ser Cys Ile Ile Thr Thr Phe Gly Val Gly Glu Leu Ser 
65                  70                  75                  80  


Ala Leu Asn Gly Ile Ala Gly Ser Tyr Ala Glu His Val Gly Val Leu 
                85                  90                  95      


His Val Val Gly Val Pro Ser Ile Ser Ala Gln Ala Lys Gln Leu Leu 
            100                 105                 110         


Leu His His Thr Leu Gly Asn Gly Asp Phe Thr Val Phe His Arg Met 
        115                 120                 125             


Ser Ala Asn Ile Ser Glu Thr Thr Ala Met Ile Thr Asp Ile Ala Thr 
    130                 135                 140                 


Ala Pro Ala Glu Ile Asp Arg Cys Ile Arg Thr Thr Tyr Val Thr Gln 
145                 150                 155                 160 


Arg Pro Val Tyr Leu Gly Leu Pro Ala Asn Leu Val Asp Leu Asn Val 
                165                 170                 175     


Pro Ala Lys Leu Leu Gln Thr Pro Ile Asp Met Ser Leu Lys Pro Asn 
            180                 185                 190         


Asp Ala Glu Ser Glu Lys Glu Val Ile Asp Thr Ile Leu Ala Leu Val 
        195                 200                 205             


Lys Asp Ala Lys Asn Pro Val Ile Leu Ala Asp Ala Cys Cys Ser Arg 
    210                 215                 220                 


His Asp Val Lys Ala Glu Thr Lys Lys Leu Ile Asp Leu Thr Gln Phe 
225                 230                 235                 240 


Pro Ala Phe Val Thr Pro Met Gly Lys Gly Ser Ile Asp Glu Gln His 
                245                 250                 255     


Pro Arg Tyr Gly Gly Val Tyr Val Gly Thr Leu Ser Lys Pro Glu Val 
            260                 265                 270         


Lys Glu Ala Val Glu Ser Ala Asp Leu Ile Leu Ser Val Gly Ala Leu 
        275                 280                 285             


Leu Ser Asp Phe Asn Thr Gly Ser Phe Ser Tyr Ser Tyr Lys Thr Lys 
    290                 295                 300                 


Asn Ile Val Glu Phe His Ser Asp His Met Lys Ile Arg Asn Ala Thr 
305                 310                 315                 320 


Phe Pro Gly Val Gln Met Lys Phe Val Leu Gln Lys Leu Leu Thr Thr 
                325                 330                 335     


Ile Ala Asp Ala Ala Lys Gly Tyr Lys Pro Val Ala Val Pro Ala Arg 
            340                 345                 350         


Thr Pro Ala Asn Ala Ala Val Pro Ala Ser Thr Pro Leu Lys Gln Glu 
        355                 360                 365             


Trp Met Trp Asn Gln Leu Gly Asn Phe Leu Gln Glu Gly Asp Val Val 
    370                 375                 380                 


Ile Ala Glu Thr Gly Thr Ser Ala Phe Gly Ile Asn Gln Thr Thr Phe 
385                 390                 395                 400 


Pro Asn Asn Thr Tyr Gly Ile Ser Gln Val Leu Trp Gly Ser Ile Gly 
                405                 410                 415     


Phe Thr Thr Gly Ala Thr Leu Gly Ala Ala Phe Ala Ala Glu Glu Ile 
            420                 425                 430         


Asp Pro Lys Lys Arg Val Ile Leu Phe Ile Gly Asp Gly Ser Leu Gln 
        435                 440                 445             


Leu Thr Val Gln Glu Ile Ser Thr Met Ile Arg Trp Gly Leu Lys Pro 
    450                 455                 460                 


Tyr Leu Phe Val Leu Asn Asn Asp Gly Tyr Thr Ile Glu Lys Leu Ile 
465                 470                 475                 480 


His Gly Pro Lys Ala Gln Tyr Asn Glu Ile Gln Gly Trp Asp His Leu 
                485                 490                 495     


Ser Leu Leu Pro Thr Phe Gly Ala Lys Asp Tyr Glu Thr His Arg Val 
            500                 505                 510         


Ala Thr Thr Gly Glu Trp Asp Lys Leu Thr Gln Asp Lys Ser Phe Asn 
        515                 520                 525             


Asp Asn Ser Lys Ile Arg Met Ile Glu Ile Met Leu Pro Val Phe Asp 
    530                 535                 540                 


Ala Pro Gln Asn Leu Val Glu Gln Ala Lys Leu Thr Ala Ala Thr Asn 
545                 550                 555                 560 


Ala Lys Gln 
            


<210>  35
<211>  1692
<212>  DNA
<213>  Artificial

<220>
<223>  Saccharomyces cerevisiae pyruvate decarboxylase Pdc codon 
       optimised gene

<400>  35
atgtccgaga tcactctggg caaatacctg tttgaacgtc tgaaacaggt gaacgttaat     60

accgtattcg gcctgccggg tgatttcaac ctgtccctgc tggacaaaat ctatgaagtt    120

gaaggtatgc gttgggctgg caacgctaac gagctgaacg cagcgtacgc ggcagatggt    180

tacgctcgta tcaaaggtat gtcttgtatc atcaccacct tcggtgttgg tgagctgagc    240

gccctgaacg gcatcgccgg ctcctatgca gagcacgtgg gcgtgctgca cgttgtgggt    300

gtaccgtcca tcagcgccca ggcaaaacag ctgctgctgc accacaccct gggtaacggc    360

gactttaccg ttttccatcg tatgtctgcg aacatcagcg aaactactgc aatgattact    420

gacatcgcta cggcaccggc agaaatcgac cgttgcattc gtaccacgta cgttactcag    480

cgcccggttt atctgggcct gccagccaac ctggtggatc tgaacgtccc ggctaaactg    540

ctgcagactc cgatcgatat gtctctgaaa cctaacgacg cagaatctga gaaagaagtt    600

atcgatacta ttctggctct ggtgaaagat gcaaagaacc cagttatcct ggctgacgca    660

tgttgctctc gtcatgatgt aaaggcagaa accaaaaagc tgatcgacct gacgcagttc    720

ccggcgttcg ttaccccgat gggcaagggt tccatcgatg agcagcaccc gcgttatggt    780

ggtgtatacg ttggcacgct gtccaaaccg gaggtaaaag aagcggttga aagcgcagat    840

ctgatcctgt ctgttggtgc actgctgagc gacttcaaca ccggttcttt ctcctatagc    900

tacaagacca aaaacattgt ggagtttcac tccgatcaca tgaaaatccg caacgcgacc    960

tttcctggtg tgcagatgaa attcgtactg cagaaactgc tgaccaccat cgccgacgct   1020

gcgaaaggtt ataaaccggt agctgtgccg gcacgtaccc cggcgaacgc cgcggttcct   1080

gcatccactc cactgaagca ggaatggatg tggaatcagc tgggtaattt cctgcaagaa   1140

ggcgacgttg taatcgcaga aaccggcact agcgcgtttg gcattaacca gacgaccttc   1200

ccaaacaaca cctacggtat cagccaagtc ctgtggggct ctatcggctt caccaccggt   1260

gcaaccctgg gtgcggcttt cgctgctgag gagatcgacc cgaagaaacg tgttatcctg   1320

ttcatcggtg acggctccct gcagctgacc gtccaggaga tttctaccat gatccgctgg   1380

ggcctgaaac cgtacctgtt tgtgctgaac aacgacggct acactattga gaaactgatc   1440

cacggtccga aagcacagta taatgagatc cagggttggg atcatctgtc tctgctgccg   1500

acctttggcg ctaaagacta cgagacccac cgcgtggcta ccaccggcga gtgggataaa   1560

ctgacgcagg ataaatcctt caatgacaat agcaagattc gtatgatcga aatcatgctg   1620

ccggtctttg atgctccgca gaacctggta gagcaagcaa aactgaccgc ggcaactaac   1680

gctaaacagt aa                                                       1692


<210>  36
<211>  1707
<212>  DNA
<213>  Zymomonas mobilis


<220>
<221>  CDS
<222>  (1)..(1707)

<400>  36
atg agt tat act gtc ggt acc tat tta gcg gag cgg ctt gtc cag att       48
Met Ser Tyr Thr Val Gly Thr Tyr Leu Ala Glu Arg Leu Val Gln Ile         
1               5                   10                  15              

ggt ctc aag cat cac ttc gca gtc gcg ggc gac tac aac ctc gtc ctt       96
Gly Leu Lys His His Phe Ala Val Ala Gly Asp Tyr Asn Leu Val Leu         
            20                  25                  30                  

ctt gac aac ctg ctt ttg aac aaa aac atg gag cag gtt tat tgc tgt      144
Leu Asp Asn Leu Leu Leu Asn Lys Asn Met Glu Gln Val Tyr Cys Cys         
        35                  40                  45                      

aac gaa ctg aac tgc ggt ttc agt gca gaa ggt tat gct cgt gcc aaa      192
Asn Glu Leu Asn Cys Gly Phe Ser Ala Glu Gly Tyr Ala Arg Ala Lys         
    50                  55                  60                          

ggc gca gca gca gcc gtc gtt acc tac agc gtc ggt gcg ctt tcc gca      240
Gly Ala Ala Ala Ala Val Val Thr Tyr Ser Val Gly Ala Leu Ser Ala         
65                  70                  75                  80          

ttt gat gct atc ggt ggc gcc tat gca gaa aac ctt ccg gtt atc ctg      288
Phe Asp Ala Ile Gly Gly Ala Tyr Ala Glu Asn Leu Pro Val Ile Leu         
                85                  90                  95              

atc tcc ggt gct ccg aac aac aat gat cac gct gct ggt cac gtg ttg      336
Ile Ser Gly Ala Pro Asn Asn Asn Asp His Ala Ala Gly His Val Leu         
            100                 105                 110                 

cat cac gct ctt ggc aaa acc gac tat cac tat cag ttg gaa atg gcc      384
His His Ala Leu Gly Lys Thr Asp Tyr His Tyr Gln Leu Glu Met Ala         
        115                 120                 125                     

aag aac atc acg gcc gcc gct gaa gcg att tac acc ccg gaa gaa gct      432
Lys Asn Ile Thr Ala Ala Ala Glu Ala Ile Tyr Thr Pro Glu Glu Ala         
    130                 135                 140                         

ccg gct aaa atc gat cac gtg att aaa act gct ctt cgt gag aag aag      480
Pro Ala Lys Ile Asp His Val Ile Lys Thr Ala Leu Arg Glu Lys Lys         
145                 150                 155                 160         

ccg gtt tat ctc gaa atc gct tgc aac att gct tcc atg ccc tgc gcc      528
Pro Val Tyr Leu Glu Ile Ala Cys Asn Ile Ala Ser Met Pro Cys Ala         
                165                 170                 175             

gct cct gga ccg gca agc gca ttg ttc aat gac gaa gcc agc gac gaa      576
Ala Pro Gly Pro Ala Ser Ala Leu Phe Asn Asp Glu Ala Ser Asp Glu         
            180                 185                 190                 

gct tct ttg aat gca gcg gtt gaa gaa acc ctg aaa ttc atc gcc aac      624
Ala Ser Leu Asn Ala Ala Val Glu Glu Thr Leu Lys Phe Ile Ala Asn         
        195                 200                 205                     

cgc gac aaa gtt gcc gtc ctc gtc ggc agc aag ctg cgc gca gct ggt      672
Arg Asp Lys Val Ala Val Leu Val Gly Ser Lys Leu Arg Ala Ala Gly         
    210                 215                 220                         

gct gaa gaa gct gct gtc aaa ttt gct gat gct ctc ggt ggc gca gtt      720
Ala Glu Glu Ala Ala Val Lys Phe Ala Asp Ala Leu Gly Gly Ala Val         
225                 230                 235                 240         

gct acc atg gct gct gca aaa agc ttc ttc cca gaa gaa aac ccg cat      768
Ala Thr Met Ala Ala Ala Lys Ser Phe Phe Pro Glu Glu Asn Pro His         
                245                 250                 255             

tac atc ggc acc tca tgg ggt gaa gtc agc tat ccg ggc gtt gaa aag      816
Tyr Ile Gly Thr Ser Trp Gly Glu Val Ser Tyr Pro Gly Val Glu Lys         
            260                 265                 270                 

acg atg aaa gaa gcc gat gcg gtt atc gct ctg gct cct gtc ttc aac      864
Thr Met Lys Glu Ala Asp Ala Val Ile Ala Leu Ala Pro Val Phe Asn         
        275                 280                 285                     

gac tac tcc acc act ggt tgg acg gat att cct gat cct aag aaa ctg      912
Asp Tyr Ser Thr Thr Gly Trp Thr Asp Ile Pro Asp Pro Lys Lys Leu         
    290                 295                 300                         

gtt ctc gct gaa ccg cgt tct gtc gtc gtt aac ggc att cgc ttc ccc      960
Val Leu Ala Glu Pro Arg Ser Val Val Val Asn Gly Ile Arg Phe Pro         
305                 310                 315                 320         

agc gtc cat ctg aaa gac tat ctg acc cgt ttg gct cag aaa gtt tcc     1008
Ser Val His Leu Lys Asp Tyr Leu Thr Arg Leu Ala Gln Lys Val Ser         
                325                 330                 335             

aag aaa acc ggt gca ttg gac ttc ttc aaa tcc ctc aat gca ggt gaa     1056
Lys Lys Thr Gly Ala Leu Asp Phe Phe Lys Ser Leu Asn Ala Gly Glu         
            340                 345                 350                 

ctg aag aaa gcc gct ccg gct gat ccg agt gct ccg ttg gtc aac gca     1104
Leu Lys Lys Ala Ala Pro Ala Asp Pro Ser Ala Pro Leu Val Asn Ala         
        355                 360                 365                     

gaa atc gcc cgt cag gtc gaa gct ctt ctg acc ccg aac acg acg gtt     1152
Glu Ile Ala Arg Gln Val Glu Ala Leu Leu Thr Pro Asn Thr Thr Val         
    370                 375                 380                         

att gct gaa acc ggt gac tct tgg ttc aat gct cag cgc atg aag ctc     1200
Ile Ala Glu Thr Gly Asp Ser Trp Phe Asn Ala Gln Arg Met Lys Leu         
385                 390                 395                 400         

ccg aac ggt gct cgc gtt gaa tat gaa atg cag tgg ggt cac att ggt     1248
Pro Asn Gly Ala Arg Val Glu Tyr Glu Met Gln Trp Gly His Ile Gly         
                405                 410                 415             

tgg tcc gtt cct gcc gcc ttc ggt tat gcc gtc ggt gct ccg gaa cgt     1296
Trp Ser Val Pro Ala Ala Phe Gly Tyr Ala Val Gly Ala Pro Glu Arg         
            420                 425                 430                 

cgc aac atc ctc atg gtt ggt gat ggt tcc ttc cag ctg acg gct cag     1344
Arg Asn Ile Leu Met Val Gly Asp Gly Ser Phe Gln Leu Thr Ala Gln         
        435                 440                 445                     

gaa gtc gct cag atg gtt cgc ctg aaa ctg ccg gtt atc atc ttc ttg     1392
Glu Val Ala Gln Met Val Arg Leu Lys Leu Pro Val Ile Ile Phe Leu         
    450                 455                 460                         

atc aat aac tat ggt tac acc gcc gaa gtt atg atc cat gat ggt ccg     1440
Ile Asn Asn Tyr Gly Tyr Thr Ala Glu Val Met Ile His Asp Gly Pro         
465                 470                 475                 480         

tac aac aac atc aag aac tgg gat tat gcc ggt ctg atg gaa gtg ttc     1488
Tyr Asn Asn Ile Lys Asn Trp Asp Tyr Ala Gly Leu Met Glu Val Phe         
                485                 490                 495             

aac ggt aac ggt ggt tat gac agc ggt gct ggt aaa ggc ctg aag gct     1536
Asn Gly Asn Gly Gly Tyr Asp Ser Gly Ala Gly Lys Gly Leu Lys Ala         
            500                 505                 510                 

aaa acc ggt ggc gaa ctg gca gaa gct atc aag gtt gct ctg gca aac     1584
Lys Thr Gly Gly Glu Leu Ala Glu Ala Ile Lys Val Ala Leu Ala Asn         
        515                 520                 525                     

acc gac ggc cca acc ctg atc gaa tgc ttc atc ggt cgt gaa gac tgc     1632
Thr Asp Gly Pro Thr Leu Ile Glu Cys Phe Ile Gly Arg Glu Asp Cys         
    530                 535                 540                         

act gaa gaa ttg gtc aaa tgg ggt aag cgc gtt gct gcc gcc aac agc     1680
Thr Glu Glu Leu Val Lys Trp Gly Lys Arg Val Ala Ala Ala Asn Ser         
545                 550                 555                 560         

cgt aag cct gtt aac aag ctc ctc tag                                 1707
Arg Lys Pro Val Asn Lys Leu Leu                                         
                565                                                     


<210>  37
<211>  568
<212>  PRT
<213>  Zymomonas mobilis

<400>  37

Met Ser Tyr Thr Val Gly Thr Tyr Leu Ala Glu Arg Leu Val Gln Ile 
1               5                   10                  15      


Gly Leu Lys His His Phe Ala Val Ala Gly Asp Tyr Asn Leu Val Leu 
            20                  25                  30          


Leu Asp Asn Leu Leu Leu Asn Lys Asn Met Glu Gln Val Tyr Cys Cys 
        35                  40                  45              


Asn Glu Leu Asn Cys Gly Phe Ser Ala Glu Gly Tyr Ala Arg Ala Lys 
    50                  55                  60                  


Gly Ala Ala Ala Ala Val Val Thr Tyr Ser Val Gly Ala Leu Ser Ala 
65                  70                  75                  80  


Phe Asp Ala Ile Gly Gly Ala Tyr Ala Glu Asn Leu Pro Val Ile Leu 
                85                  90                  95      


Ile Ser Gly Ala Pro Asn Asn Asn Asp His Ala Ala Gly His Val Leu 
            100                 105                 110         


His His Ala Leu Gly Lys Thr Asp Tyr His Tyr Gln Leu Glu Met Ala 
        115                 120                 125             


Lys Asn Ile Thr Ala Ala Ala Glu Ala Ile Tyr Thr Pro Glu Glu Ala 
    130                 135                 140                 


Pro Ala Lys Ile Asp His Val Ile Lys Thr Ala Leu Arg Glu Lys Lys 
145                 150                 155                 160 


Pro Val Tyr Leu Glu Ile Ala Cys Asn Ile Ala Ser Met Pro Cys Ala 
                165                 170                 175     


Ala Pro Gly Pro Ala Ser Ala Leu Phe Asn Asp Glu Ala Ser Asp Glu 
            180                 185                 190         


Ala Ser Leu Asn Ala Ala Val Glu Glu Thr Leu Lys Phe Ile Ala Asn 
        195                 200                 205             


Arg Asp Lys Val Ala Val Leu Val Gly Ser Lys Leu Arg Ala Ala Gly 
    210                 215                 220                 


Ala Glu Glu Ala Ala Val Lys Phe Ala Asp Ala Leu Gly Gly Ala Val 
225                 230                 235                 240 


Ala Thr Met Ala Ala Ala Lys Ser Phe Phe Pro Glu Glu Asn Pro His 
                245                 250                 255     


Tyr Ile Gly Thr Ser Trp Gly Glu Val Ser Tyr Pro Gly Val Glu Lys 
            260                 265                 270         


Thr Met Lys Glu Ala Asp Ala Val Ile Ala Leu Ala Pro Val Phe Asn 
        275                 280                 285             


Asp Tyr Ser Thr Thr Gly Trp Thr Asp Ile Pro Asp Pro Lys Lys Leu 
    290                 295                 300                 


Val Leu Ala Glu Pro Arg Ser Val Val Val Asn Gly Ile Arg Phe Pro 
305                 310                 315                 320 


Ser Val His Leu Lys Asp Tyr Leu Thr Arg Leu Ala Gln Lys Val Ser 
                325                 330                 335     


Lys Lys Thr Gly Ala Leu Asp Phe Phe Lys Ser Leu Asn Ala Gly Glu 
            340                 345                 350         


Leu Lys Lys Ala Ala Pro Ala Asp Pro Ser Ala Pro Leu Val Asn Ala 
        355                 360                 365             


Glu Ile Ala Arg Gln Val Glu Ala Leu Leu Thr Pro Asn Thr Thr Val 
    370                 375                 380                 


Ile Ala Glu Thr Gly Asp Ser Trp Phe Asn Ala Gln Arg Met Lys Leu 
385                 390                 395                 400 


Pro Asn Gly Ala Arg Val Glu Tyr Glu Met Gln Trp Gly His Ile Gly 
                405                 410                 415     


Trp Ser Val Pro Ala Ala Phe Gly Tyr Ala Val Gly Ala Pro Glu Arg 
            420                 425                 430         


Arg Asn Ile Leu Met Val Gly Asp Gly Ser Phe Gln Leu Thr Ala Gln 
        435                 440                 445             


Glu Val Ala Gln Met Val Arg Leu Lys Leu Pro Val Ile Ile Phe Leu 
    450                 455                 460                 


Ile Asn Asn Tyr Gly Tyr Thr Ala Glu Val Met Ile His Asp Gly Pro 
465                 470                 475                 480 


Tyr Asn Asn Ile Lys Asn Trp Asp Tyr Ala Gly Leu Met Glu Val Phe 
                485                 490                 495     


Asn Gly Asn Gly Gly Tyr Asp Ser Gly Ala Gly Lys Gly Leu Lys Ala 
            500                 505                 510         


Lys Thr Gly Gly Glu Leu Ala Glu Ala Ile Lys Val Ala Leu Ala Asn 
        515                 520                 525             


Thr Asp Gly Pro Thr Leu Ile Glu Cys Phe Ile Gly Arg Glu Asp Cys 
    530                 535                 540                 


Thr Glu Glu Leu Val Lys Trp Gly Lys Arg Val Ala Ala Ala Asn Ser 
545                 550                 555                 560 


Arg Lys Pro Val Asn Lys Leu Leu 
                565             


<210>  38
<211>  1707
<212>  DNA
<213>  Artificial

<220>
<223>  Zymomonas mobilis pyruvate decarboxylase PdcI472A codon optimised
       gene

<400>  38
atgtcttata ctgttggtac ttatctggct gagcgtctgg tgcaaatcgg cctgaaacac     60

cactttgcag ttgctggcga ctacaacctg gttctgctgg ataacctgct gctgaacaaa    120

aacatggagc aagtttattg ctgtaacgag ctgaactgcg gcttctctgc ggagggttat    180

gcgcgtgcga aaggtgccgc tgcagcagtc gtaacctact ctgtgggcgc tctgtccgcg    240

ttcgacgcaa tcggtggcgc ttacgctgaa aacctgccgg tgatcctgat tagcggtgcg    300

ccgaataata acgaccatgc tgctggccac gttctgcacc acgccctggg taaaactgat    360

taccattacc agctggagat ggctaaaaac atcactgcag cagcagaagc gatctacacc    420

ccggaagagg ctccggcaaa aatcgaccac gtgattaaaa ccgctctgcg tgagaaaaag    480

ccggtatacc tggaaatcgc gtgcaacatc gcgtctatgc cgtgcgccgc accgggtccg    540

gcttctgccc tgttcaacga tgaggcgagc gatgaggcat ctctgaacgc agcagtagaa    600

gaaaccctga aatttatcgc aaaccgtgac aaagtagcag tcctggtagg ttctaaactg    660

cgtgcggctg gtgcggaaga ggctgcggta aagttcgcgg atgctctggg cggtgcagtg    720

gcgaccatgg cagcggctaa atccttcttc ccagaggaga acccgcatta cattggtacc    780

tcctggggcg aagtttccta ccctggtgtg gagaaaacca tgaaagaagc cgatgctgtg    840

attgccctgg cgcctgtatt caacgattat tccaccaccg gttggaccga tatcccggac    900

ccgaagaaac tggtcctggc tgaaccgcgc tccgtagtag tgaatggcat tcgtttcccg    960

tccgtacacc tgaaggatta cctgacgcgt ctggcacaga aagtatccaa gaaaactggc   1020

gcgctggact tctttaaatc cctgaacgct ggtgagctga aaaaggcggc tccggccgat   1080

ccgtccgcac cgctggtgaa cgcagagatt gcacgtcagg ttgaggcact gctgacgccg   1140

aacaccaccg taatcgcgga aacgggcgac tcttggttca acgcacagcg catgaaactg   1200

ccgaacggtg cccgcgttga atatgaaatg cagtggggtc acatcggctg gtctgtccca   1260

gcagcgtttg gttacgcggt tggtgcaccg gagcgtcgca acatcctgat ggtgggtgac   1320

ggctccttcc agctgactgc tcaggaggtg gcgcagatgg tgcgcctgaa gctgccggtt   1380

atcattttcc tgatcaacaa ctacggctac accgccgagg taatgatcca cgatggtccg   1440

tacaacaaca tcaaaaactg ggactacgcc ggtctgatgg aggtttttaa cggtaacggc   1500

ggttacgaca gcggtgctgg taagggtctg aaagccaaaa ccggtggcga actggcagag   1560

gcgattaaag ttgcgctggc aaacaccgat ggcccgaccc tgatcgagtg cttcatcggc   1620

cgtgaggact gcaccgagga gctggtcaaa tggggcaaac gtgtggcggc tgctaactct   1680

cgcaagccgg taaacaaact gctgtaa                                       1707


<210>  39
<211>  1644
<212>  DNA
<213>  Lactococcus lactis


<220>
<221>  CDS
<222>  (1)..(1644)

<400>  39
atg tat aca gta gga gat tac ctg tta gac cga tta cac gag ttg gga       48
Met Tyr Thr Val Gly Asp Tyr Leu Leu Asp Arg Leu His Glu Leu Gly         
1               5                   10                  15              

att gaa gaa att ttt gga gtt cct ggt gac tat aac tta caa ttt tta       96
Ile Glu Glu Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu Gln Phe Leu         
            20                  25                  30                  

gat caa att att tca cgc gaa gat atg aaa tgg att gga aat gct aat      144
Asp Gln Ile Ile Ser Arg Glu Asp Met Lys Trp Ile Gly Asn Ala Asn         
        35                  40                  45                      

gaa tta aat gct tct tat atg gct gat ggt tat gct cgt act aaa aaa      192
Glu Leu Asn Ala Ser Tyr Met Ala Asp Gly Tyr Ala Arg Thr Lys Lys         
    50                  55                  60                          

gct gcc gca ttt ctc acc aca ttt gga gtc ggc gaa ttg agt gcg atc      240
Ala Ala Ala Phe Leu Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Ile         
65                  70                  75                  80          

aat gga ctg gca gga agt tat gcc gaa aat tta cca gta gta gaa att      288
Asn Gly Leu Ala Gly Ser Tyr Ala Glu Asn Leu Pro Val Val Glu Ile         
                85                  90                  95              

gtt ggt tca cca act tca aaa gta caa aat gac gga aaa ttt gtc cat      336
Val Gly Ser Pro Thr Ser Lys Val Gln Asn Asp Gly Lys Phe Val His         
            100                 105                 110                 

cat aca cta gca gat ggt gat ttt aaa cac ttt atg aag atg cat gaa      384
His Thr Leu Ala Asp Gly Asp Phe Lys His Phe Met Lys Met His Glu         
        115                 120                 125                     

cct gtt aca gca gcg cgg act tta ctg aca gca gaa aat gcc aca tat      432
Pro Val Thr Ala Ala Arg Thr Leu Leu Thr Ala Glu Asn Ala Thr Tyr         
    130                 135                 140                         

gaa att gac cga gta ctt tct caa tta cta aaa gaa aga aaa cca gtc      480
Glu Ile Asp Arg Val Leu Ser Gln Leu Leu Lys Glu Arg Lys Pro Val         
145                 150                 155                 160         

tat att aac tta cca gtc gat gtt gct gca gca aaa gca gag aag cct      528
Tyr Ile Asn Leu Pro Val Asp Val Ala Ala Ala Lys Ala Glu Lys Pro         
                165                 170                 175             

gca tta tct tta gaa aaa gaa agc tct aca aca aat aca act gaa caa      576
Ala Leu Ser Leu Glu Lys Glu Ser Ser Thr Thr Asn Thr Thr Glu Gln         
            180                 185                 190                 

gtg att ttg agt aag att gaa gaa agt ttg aaa aat gcc caa aaa cca      624
Val Ile Leu Ser Lys Ile Glu Glu Ser Leu Lys Asn Ala Gln Lys Pro         
        195                 200                 205                     

gta gtg att gca gga cac gaa gta att agt ttt ggt tta gaa aaa acg      672
Val Val Ile Ala Gly His Glu Val Ile Ser Phe Gly Leu Glu Lys Thr         
    210                 215                 220                         

gta act cag ttt gtt tca gaa aca aaa cta ccg att acg aca cta aat      720
Val Thr Gln Phe Val Ser Glu Thr Lys Leu Pro Ile Thr Thr Leu Asn         
225                 230                 235                 240         

ttt ggt aaa agt gct gtt gat gaa tct ttg ccc tca ttt tta gga ata      768
Phe Gly Lys Ser Ala Val Asp Glu Ser Leu Pro Ser Phe Leu Gly Ile         
                245                 250                 255             

tat aac ggg aaa ctt tca gaa atc agt ctt aaa aat ttt gtg gag tcc      816
Tyr Asn Gly Lys Leu Ser Glu Ile Ser Leu Lys Asn Phe Val Glu Ser         
            260                 265                 270                 

gca gac ttt atc cta atg ctt gga gtg aag ctt acg gac tcc tca aca      864
Ala Asp Phe Ile Leu Met Leu Gly Val Lys Leu Thr Asp Ser Ser Thr         
        275                 280                 285                     

ggt gca ttc aca cat cat tta gat gaa aat aaa atg att tca cta aac      912
Gly Ala Phe Thr His His Leu Asp Glu Asn Lys Met Ile Ser Leu Asn         
    290                 295                 300                         

ata gat gaa gga ata att ttc aat aaa gtg gta gaa gat ttt gat ttt      960
Ile Asp Glu Gly Ile Ile Phe Asn Lys Val Val Glu Asp Phe Asp Phe         
305                 310                 315                 320         

aga gca gtg gtt tct tct tta tca gaa tta aaa gga ata gaa tat gaa     1008
Arg Ala Val Val Ser Ser Leu Ser Glu Leu Lys Gly Ile Glu Tyr Glu         
                325                 330                 335             

gga caa tat att gat aag caa tat gaa gaa ttt att cca tca agt gct     1056
Gly Gln Tyr Ile Asp Lys Gln Tyr Glu Glu Phe Ile Pro Ser Ser Ala         
            340                 345                 350                 

ccc tta tca caa gac cgt cta tgg cag gca gtt gaa agt ttg act caa     1104
Pro Leu Ser Gln Asp Arg Leu Trp Gln Ala Val Glu Ser Leu Thr Gln         
        355                 360                 365                     

agc aat gaa aca atc gtt gct gaa caa gga acc tca ttt ttt gga gct     1152
Ser Asn Glu Thr Ile Val Ala Glu Gln Gly Thr Ser Phe Phe Gly Ala         
    370                 375                 380                         

tca aca att ttc tta aaa tca aat agt cgt ttt att gga caa cct tta     1200
Ser Thr Ile Phe Leu Lys Ser Asn Ser Arg Phe Ile Gly Gln Pro Leu         
385                 390                 395                 400         

tgg ggt tct att gga tat act ttt cca gcg gct tta gga agc caa att     1248
Trp Gly Ser Ile Gly Tyr Thr Phe Pro Ala Ala Leu Gly Ser Gln Ile         
                405                 410                 415             

gcg gat aaa gag agc aga cac ctt tta ttt att ggt gat ggt tca ctt     1296
Ala Asp Lys Glu Ser Arg His Leu Leu Phe Ile Gly Asp Gly Ser Leu         
            420                 425                 430                 

caa ctt acc gta caa gaa tta gga cta tca atc aga gaa aaa ctc aat     1344
Gln Leu Thr Val Gln Glu Leu Gly Leu Ser Ile Arg Glu Lys Leu Asn         
        435                 440                 445                     

cca att tgt ttt atc ata aat aat gat ggt tat aca gtt gaa aga gaa     1392
Pro Ile Cys Phe Ile Ile Asn Asn Asp Gly Tyr Thr Val Glu Arg Glu         
    450                 455                 460                         

atc cac gga cct act caa agt tat aac gac att cca atg tgg aat tac     1440
Ile His Gly Pro Thr Gln Ser Tyr Asn Asp Ile Pro Met Trp Asn Tyr         
465                 470                 475                 480         

tcg aaa tta cca gaa aca ttt gga gca aca gaa gat cgt gta gta tca     1488
Ser Lys Leu Pro Glu Thr Phe Gly Ala Thr Glu Asp Arg Val Val Ser         
                485                 490                 495             

aaa att gtt aga aca gag aat gaa ttt gtg tct gtc atg aaa gaa gcc     1536
Lys Ile Val Arg Thr Glu Asn Glu Phe Val Ser Val Met Lys Glu Ala         
            500                 505                 510                 

caa gca gat gtc aat aga atg tat tgg ata gaa cta gtt ttg gaa aaa     1584
Gln Ala Asp Val Asn Arg Met Tyr Trp Ile Glu Leu Val Leu Glu Lys         
        515                 520                 525                     

gaa gat gcg cca aaa tta ctg aaa aaa atg ggt aaa tta ttt gct gag     1632
Glu Asp Ala Pro Lys Leu Leu Lys Lys Met Gly Lys Leu Phe Ala Glu         
    530                 535                 540                         

caa aat aaa tag                                                     1644
Gln Asn Lys                                                             
545                                                                     


<210>  40
<211>  547
<212>  PRT
<213>  Lactococcus lactis

<400>  40

Met Tyr Thr Val Gly Asp Tyr Leu Leu Asp Arg Leu His Glu Leu Gly 
1               5                   10                  15      


Ile Glu Glu Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu Gln Phe Leu 
            20                  25                  30          


Asp Gln Ile Ile Ser Arg Glu Asp Met Lys Trp Ile Gly Asn Ala Asn 
        35                  40                  45              


Glu Leu Asn Ala Ser Tyr Met Ala Asp Gly Tyr Ala Arg Thr Lys Lys 
    50                  55                  60                  


Ala Ala Ala Phe Leu Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Ile 
65                  70                  75                  80  


Asn Gly Leu Ala Gly Ser Tyr Ala Glu Asn Leu Pro Val Val Glu Ile 
                85                  90                  95      


Val Gly Ser Pro Thr Ser Lys Val Gln Asn Asp Gly Lys Phe Val His 
            100                 105                 110         


His Thr Leu Ala Asp Gly Asp Phe Lys His Phe Met Lys Met His Glu 
        115                 120                 125             


Pro Val Thr Ala Ala Arg Thr Leu Leu Thr Ala Glu Asn Ala Thr Tyr 
    130                 135                 140                 


Glu Ile Asp Arg Val Leu Ser Gln Leu Leu Lys Glu Arg Lys Pro Val 
145                 150                 155                 160 


Tyr Ile Asn Leu Pro Val Asp Val Ala Ala Ala Lys Ala Glu Lys Pro 
                165                 170                 175     


Ala Leu Ser Leu Glu Lys Glu Ser Ser Thr Thr Asn Thr Thr Glu Gln 
            180                 185                 190         


Val Ile Leu Ser Lys Ile Glu Glu Ser Leu Lys Asn Ala Gln Lys Pro 
        195                 200                 205             


Val Val Ile Ala Gly His Glu Val Ile Ser Phe Gly Leu Glu Lys Thr 
    210                 215                 220                 


Val Thr Gln Phe Val Ser Glu Thr Lys Leu Pro Ile Thr Thr Leu Asn 
225                 230                 235                 240 


Phe Gly Lys Ser Ala Val Asp Glu Ser Leu Pro Ser Phe Leu Gly Ile 
                245                 250                 255     


Tyr Asn Gly Lys Leu Ser Glu Ile Ser Leu Lys Asn Phe Val Glu Ser 
            260                 265                 270         


Ala Asp Phe Ile Leu Met Leu Gly Val Lys Leu Thr Asp Ser Ser Thr 
        275                 280                 285             


Gly Ala Phe Thr His His Leu Asp Glu Asn Lys Met Ile Ser Leu Asn 
    290                 295                 300                 


Ile Asp Glu Gly Ile Ile Phe Asn Lys Val Val Glu Asp Phe Asp Phe 
305                 310                 315                 320 


Arg Ala Val Val Ser Ser Leu Ser Glu Leu Lys Gly Ile Glu Tyr Glu 
                325                 330                 335     


Gly Gln Tyr Ile Asp Lys Gln Tyr Glu Glu Phe Ile Pro Ser Ser Ala 
            340                 345                 350         


Pro Leu Ser Gln Asp Arg Leu Trp Gln Ala Val Glu Ser Leu Thr Gln 
        355                 360                 365             


Ser Asn Glu Thr Ile Val Ala Glu Gln Gly Thr Ser Phe Phe Gly Ala 
    370                 375                 380                 


Ser Thr Ile Phe Leu Lys Ser Asn Ser Arg Phe Ile Gly Gln Pro Leu 
385                 390                 395                 400 


Trp Gly Ser Ile Gly Tyr Thr Phe Pro Ala Ala Leu Gly Ser Gln Ile 
                405                 410                 415     


Ala Asp Lys Glu Ser Arg His Leu Leu Phe Ile Gly Asp Gly Ser Leu 
            420                 425                 430         


Gln Leu Thr Val Gln Glu Leu Gly Leu Ser Ile Arg Glu Lys Leu Asn 
        435                 440                 445             


Pro Ile Cys Phe Ile Ile Asn Asn Asp Gly Tyr Thr Val Glu Arg Glu 
    450                 455                 460                 


Ile His Gly Pro Thr Gln Ser Tyr Asn Asp Ile Pro Met Trp Asn Tyr 
465                 470                 475                 480 


Ser Lys Leu Pro Glu Thr Phe Gly Ala Thr Glu Asp Arg Val Val Ser 
                485                 490                 495     


Lys Ile Val Arg Thr Glu Asn Glu Phe Val Ser Val Met Lys Glu Ala 
            500                 505                 510         


Gln Ala Asp Val Asn Arg Met Tyr Trp Ile Glu Leu Val Leu Glu Lys 
        515                 520                 525             


Glu Asp Ala Pro Lys Leu Leu Lys Lys Met Gly Lys Leu Phe Ala Glu 
    530                 535                 540                 


Gln Asn Lys 
545         


<210>  41
<211>  1644
<212>  DNA
<213>  Artificial

<220>
<223>  Lactococcus lactis branched chain alpha-ketoacid decarboxylase 
       KdcA codon optimised gene

<400>  41
atgtatactg ttggtgatta tctgctggac cgtctgcatg aactgggcat tgaagaaatc     60

ttcggtgtcc caggcgacta caacctgcag ttcctggacc agatcatctc ccgcgaagat    120

atgaaatgga tcggtaacgc aaacgagctg aacgcgtctt atatggctga tggttatgct    180

cgcaccaaaa aggctgcggc ctttctgacc acctttggtg tgggcgagct gagcgcgatc    240

aacggcctgg caggttccta cgctgagaac ctgccggtag tagaaatcgt tggttccccg    300

acctctaagg ttcagaacga cggcaaattc gtacatcaca ccctggcgga cggcgatttt    360

aagcacttta tgaaaatgca cgaaccggtc accgccgctc gcactctgct gaccgcggaa    420

aacgcaacgt acgagatcga tcgtgtactg tcccagctgc tgaaagaacg taaaccggtg    480

tatatcaatc tgccggttga tgtcgctgcg gccaaagcag agaaaccggc actgtccctg    540

gagaaggaga gctccactac taacaccacc gaacaggtta tcctgtccaa aattgaagaa    600

tctctgaaaa acgcacagaa accggtggtt atcgcaggtc acgaggttat ctccttcggc    660

ctggagaaaa ctgttactca attcgtctct gaaacgaaac tgccgatcac gaccctgaac    720

tttggcaagt ccgcagttga cgaatctctg ccttctttcc tgggcattta caacggcaaa    780

ctgtccgaga tctccctgaa gaacttcgta gaatccgctg actttatcct gatgctgggt    840

gtgaaactga ccgactcctc taccggtgcg ttcacgcacc atctggatga aaacaaaatg    900

atcagcctga acatcgacga gggtatcatc ttcaacaagg tagttgaaga tttcgacttc    960

cgtgctgttg tcagcagcct gtccgagctg aaaggcattg agtacgaggg tcaatacatc   1020

gataaacagt acgaagagtt tattccgtct tctgcaccgc tgagccagga ccgcctgtgg   1080

caggcagttg agtccctgac gcagtccaac gaaactatcg tagcggaaca aggtacctct   1140

ttcttcggtg cttctaccat ctttctgaag tccaactctc gctttatcgg tcagccgctg   1200

tggggttcta tcggttacac gttcccggct gcgctgggta gccagatcgc tgataaagag   1260

tctcgtcatc tgctgttcat cggtgatggt tccctgcagc tgactgtaca ggaactgggt   1320

ctgtctatcc gtgaaaaact gaacccgatt tgttttatca tcaataacga tggctacact   1380

gttgagcgtg aaattcatgg tccgactcag tcttacaacg atattccgat gtggaactac   1440

tctaaactgc cggaaacctt cggtgcaact gaggatcgcg tcgtgagcaa gattgtgcgt   1500

actgagaacg agttcgtatc tgttatgaaa gaggcgcagg cagatgtgaa ccgcatgtac   1560

tggatcgaac tggttctgga aaaagaggat gcaccgaaac tgctgaagaa aatgggtaaa   1620

ctgtttgcgg agcagaacaa gtaa                                          1644


<210>  42
<211>  1647
<212>  DNA
<213>  Lactococcus lactis


<220>
<221>  CDS
<222>  (1)..(1647)

<400>  42
atg tat aca gta gga gat tac cta tta gac cga tta cac gag tta gga       48
Met Tyr Thr Val Gly Asp Tyr Leu Leu Asp Arg Leu His Glu Leu Gly         
1               5                   10                  15              

att gaa gaa att ttt gga gtc cct gga gac tat aac tta caa ttt tta       96
Ile Glu Glu Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu Gln Phe Leu         
            20                  25                  30                  

gat caa att att tcc cac aag gat atg aaa tgg gtc gga aat gct aat      144
Asp Gln Ile Ile Ser His Lys Asp Met Lys Trp Val Gly Asn Ala Asn         
        35                  40                  45                      

gaa tta aat gct tca tat atg gct gat ggc tat gct cgt act aaa aaa      192
Glu Leu Asn Ala Ser Tyr Met Ala Asp Gly Tyr Ala Arg Thr Lys Lys         
    50                  55                  60                          

gct gcc gca ttt ctt aca acc ttt gga gta ggt gaa ttg agt gca gtt      240
Ala Ala Ala Phe Leu Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Val         
65                  70                  75                  80          

aat gga tta gca gga agt tac gcc gaa aat tta cca gta gta gaa ata      288
Asn Gly Leu Ala Gly Ser Tyr Ala Glu Asn Leu Pro Val Val Glu Ile         
                85                  90                  95              

gtg gga tca cct aca tca aaa gtt caa aat gaa gga aaa ttt gtt cat      336
Val Gly Ser Pro Thr Ser Lys Val Gln Asn Glu Gly Lys Phe Val His         
            100                 105                 110                 

cat acg ctg gct gac ggt gat ttt aaa cac ttt atg aaa atg cac gaa      384
His Thr Leu Ala Asp Gly Asp Phe Lys His Phe Met Lys Met His Glu         
        115                 120                 125                     

cct gtt aca gca gct cga act tta ctg aca gca gaa aat gca acc gtt      432
Pro Val Thr Ala Ala Arg Thr Leu Leu Thr Ala Glu Asn Ala Thr Val         
    130                 135                 140                         

gaa att gac cga gta ctt tct gca cta tta aaa gaa aga aaa cct gtc      480
Glu Ile Asp Arg Val Leu Ser Ala Leu Leu Lys Glu Arg Lys Pro Val         
145                 150                 155                 160         

tat atc aac tta cca gtt gat gtt gct gct gca aaa gca gag aaa ccc      528
Tyr Ile Asn Leu Pro Val Asp Val Ala Ala Ala Lys Ala Glu Lys Pro         
                165                 170                 175             

tca ctc cct ttg aaa aag gaa aac tca act tca aat aca agt gac caa      576
Ser Leu Pro Leu Lys Lys Glu Asn Ser Thr Ser Asn Thr Ser Asp Gln         
            180                 185                 190                 

gaa att ttg aac aaa att caa gaa agc ttg aaa aat gcc aaa aaa cca      624
Glu Ile Leu Asn Lys Ile Gln Glu Ser Leu Lys Asn Ala Lys Lys Pro         
        195                 200                 205                     

atc gtg att aca gga cat gaa ata att agt ttt ggc tta gaa aaa aca      672
Ile Val Ile Thr Gly His Glu Ile Ile Ser Phe Gly Leu Glu Lys Thr         
    210                 215                 220                         

gtc act caa ttt att tca aag aca aaa cta cct att acg aca tta aac      720
Val Thr Gln Phe Ile Ser Lys Thr Lys Leu Pro Ile Thr Thr Leu Asn         
225                 230                 235                 240         

ttt ggt aaa agt tca gtt gat gaa gcc ctc cct tca ttt tta gga atc      768
Phe Gly Lys Ser Ser Val Asp Glu Ala Leu Pro Ser Phe Leu Gly Ile         
                245                 250                 255             

tat aat ggt aca ctc tca gag cct aat ctt aaa gaa ttc gtg gaa tca      816
Tyr Asn Gly Thr Leu Ser Glu Pro Asn Leu Lys Glu Phe Val Glu Ser         
            260                 265                 270                 

gcc gac ttc atc ttg atg ctt gga gtt aaa ctc aca gac tct tca aca      864
Ala Asp Phe Ile Leu Met Leu Gly Val Lys Leu Thr Asp Ser Ser Thr         
        275                 280                 285                     

gga gcc ttc act cat cat tta aat gaa aat aaa atg att tca ctg aat      912
Gly Ala Phe Thr His His Leu Asn Glu Asn Lys Met Ile Ser Leu Asn         
    290                 295                 300                         

ata gat gaa gga aaa ata ttt aac gaa aga atc caa aat ttt gat ttt      960
Ile Asp Glu Gly Lys Ile Phe Asn Glu Arg Ile Gln Asn Phe Asp Phe         
305                 310                 315                 320         

gaa tcc ctc atc tcc tct ctc tta gac cta agc gaa ata gaa tac aaa     1008
Glu Ser Leu Ile Ser Ser Leu Leu Asp Leu Ser Glu Ile Glu Tyr Lys         
                325                 330                 335             

gga aaa tat atc gat aaa aag caa gaa gac ttt gtt cca tca aat gcg     1056
Gly Lys Tyr Ile Asp Lys Lys Gln Glu Asp Phe Val Pro Ser Asn Ala         
            340                 345                 350                 

ctt tta tca caa gac cgc cta tgg caa gca gtt gaa aac cta act caa     1104
Leu Leu Ser Gln Asp Arg Leu Trp Gln Ala Val Glu Asn Leu Thr Gln         
        355                 360                 365                     

agc aat gaa aca atc gtt gct gaa caa ggg aca tca ttc ttt ggc gct     1152
Ser Asn Glu Thr Ile Val Ala Glu Gln Gly Thr Ser Phe Phe Gly Ala         
    370                 375                 380                         

tca tca att ttc tta aaa tca aag agt cat ttt att ggt caa ccc tta     1200
Ser Ser Ile Phe Leu Lys Ser Lys Ser His Phe Ile Gly Gln Pro Leu         
385                 390                 395                 400         

tgg gga tca att gga tat aca ttc cca gca gca tta gga agc caa att     1248
Trp Gly Ser Ile Gly Tyr Thr Phe Pro Ala Ala Leu Gly Ser Gln Ile         
                405                 410                 415             

gca gat aaa gaa agc aga cac ctt tta ttt att ggt gat ggt tca ctt     1296
Ala Asp Lys Glu Ser Arg His Leu Leu Phe Ile Gly Asp Gly Ser Leu         
            420                 425                 430                 

caa ctt aca gtg caa gaa tta gga tta gca atc aga gaa aaa att aat     1344
Gln Leu Thr Val Gln Glu Leu Gly Leu Ala Ile Arg Glu Lys Ile Asn         
        435                 440                 445                     

cca att tgc ttt att atc aat aat gat ggt tat aca gtc gaa aga gaa     1392
Pro Ile Cys Phe Ile Ile Asn Asn Asp Gly Tyr Thr Val Glu Arg Glu         
    450                 455                 460                         

att cat gga cca aat caa agc tac aat gat att cca atg tgg aat tac     1440
Ile His Gly Pro Asn Gln Ser Tyr Asn Asp Ile Pro Met Trp Asn Tyr         
465                 470                 475                 480         

tca aaa tta cca gaa tcg ttt gga gca aca gaa gat cga gta gtc tca     1488
Ser Lys Leu Pro Glu Ser Phe Gly Ala Thr Glu Asp Arg Val Val Ser         
                485                 490                 495             

aaa atc gtt aga act gaa aat gaa ttt gtg tct gtc atg aaa gaa gct     1536
Lys Ile Val Arg Thr Glu Asn Glu Phe Val Ser Val Met Lys Glu Ala         
            500                 505                 510                 

caa gca gat cca aat aga atg tac tgg att gag tta att ttg gca aaa     1584
Gln Ala Asp Pro Asn Arg Met Tyr Trp Ile Glu Leu Ile Leu Ala Lys         
        515                 520                 525                     

gaa ggt gca cca aaa gta ctg aaa aaa atg ggc aaa cta ttt gct gaa     1632
Glu Gly Ala Pro Lys Val Leu Lys Lys Met Gly Lys Leu Phe Ala Glu         
    530                 535                 540                         

caa aat aaa tca taa                                                 1647
Gln Asn Lys Ser                                                         
545                                                                     


<210>  43
<211>  548
<212>  PRT
<213>  Lactococcus lactis

<400>  43

Met Tyr Thr Val Gly Asp Tyr Leu Leu Asp Arg Leu His Glu Leu Gly 
1               5                   10                  15      


Ile Glu Glu Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu Gln Phe Leu 
            20                  25                  30          


Asp Gln Ile Ile Ser His Lys Asp Met Lys Trp Val Gly Asn Ala Asn 
        35                  40                  45              


Glu Leu Asn Ala Ser Tyr Met Ala Asp Gly Tyr Ala Arg Thr Lys Lys 
    50                  55                  60                  


Ala Ala Ala Phe Leu Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Val 
65                  70                  75                  80  


Asn Gly Leu Ala Gly Ser Tyr Ala Glu Asn Leu Pro Val Val Glu Ile 
                85                  90                  95      


Val Gly Ser Pro Thr Ser Lys Val Gln Asn Glu Gly Lys Phe Val His 
            100                 105                 110         


His Thr Leu Ala Asp Gly Asp Phe Lys His Phe Met Lys Met His Glu 
        115                 120                 125             


Pro Val Thr Ala Ala Arg Thr Leu Leu Thr Ala Glu Asn Ala Thr Val 
    130                 135                 140                 


Glu Ile Asp Arg Val Leu Ser Ala Leu Leu Lys Glu Arg Lys Pro Val 
145                 150                 155                 160 


Tyr Ile Asn Leu Pro Val Asp Val Ala Ala Ala Lys Ala Glu Lys Pro 
                165                 170                 175     


Ser Leu Pro Leu Lys Lys Glu Asn Ser Thr Ser Asn Thr Ser Asp Gln 
            180                 185                 190         


Glu Ile Leu Asn Lys Ile Gln Glu Ser Leu Lys Asn Ala Lys Lys Pro 
        195                 200                 205             


Ile Val Ile Thr Gly His Glu Ile Ile Ser Phe Gly Leu Glu Lys Thr 
    210                 215                 220                 


Val Thr Gln Phe Ile Ser Lys Thr Lys Leu Pro Ile Thr Thr Leu Asn 
225                 230                 235                 240 


Phe Gly Lys Ser Ser Val Asp Glu Ala Leu Pro Ser Phe Leu Gly Ile 
                245                 250                 255     


Tyr Asn Gly Thr Leu Ser Glu Pro Asn Leu Lys Glu Phe Val Glu Ser 
            260                 265                 270         


Ala Asp Phe Ile Leu Met Leu Gly Val Lys Leu Thr Asp Ser Ser Thr 
        275                 280                 285             


Gly Ala Phe Thr His His Leu Asn Glu Asn Lys Met Ile Ser Leu Asn 
    290                 295                 300                 


Ile Asp Glu Gly Lys Ile Phe Asn Glu Arg Ile Gln Asn Phe Asp Phe 
305                 310                 315                 320 


Glu Ser Leu Ile Ser Ser Leu Leu Asp Leu Ser Glu Ile Glu Tyr Lys 
                325                 330                 335     


Gly Lys Tyr Ile Asp Lys Lys Gln Glu Asp Phe Val Pro Ser Asn Ala 
            340                 345                 350         


Leu Leu Ser Gln Asp Arg Leu Trp Gln Ala Val Glu Asn Leu Thr Gln 
        355                 360                 365             


Ser Asn Glu Thr Ile Val Ala Glu Gln Gly Thr Ser Phe Phe Gly Ala 
    370                 375                 380                 


Ser Ser Ile Phe Leu Lys Ser Lys Ser His Phe Ile Gly Gln Pro Leu 
385                 390                 395                 400 


Trp Gly Ser Ile Gly Tyr Thr Phe Pro Ala Ala Leu Gly Ser Gln Ile 
                405                 410                 415     


Ala Asp Lys Glu Ser Arg His Leu Leu Phe Ile Gly Asp Gly Ser Leu 
            420                 425                 430         


Gln Leu Thr Val Gln Glu Leu Gly Leu Ala Ile Arg Glu Lys Ile Asn 
        435                 440                 445             


Pro Ile Cys Phe Ile Ile Asn Asn Asp Gly Tyr Thr Val Glu Arg Glu 
    450                 455                 460                 


Ile His Gly Pro Asn Gln Ser Tyr Asn Asp Ile Pro Met Trp Asn Tyr 
465                 470                 475                 480 


Ser Lys Leu Pro Glu Ser Phe Gly Ala Thr Glu Asp Arg Val Val Ser 
                485                 490                 495     


Lys Ile Val Arg Thr Glu Asn Glu Phe Val Ser Val Met Lys Glu Ala 
            500                 505                 510         


Gln Ala Asp Pro Asn Arg Met Tyr Trp Ile Glu Leu Ile Leu Ala Lys 
        515                 520                 525             


Glu Gly Ala Pro Lys Val Leu Lys Lys Met Gly Lys Leu Phe Ala Glu 
    530                 535                 540                 


Gln Asn Lys Ser 
545             


<210>  44
<211>  1647
<212>  DNA
<213>  Artificial

<220>
<223>  Lactococcus lactis  -ketoisovalerate decarboxylase KivD codon 
       optimised gene

<400>  44
atgtatactg ttggtgatta cctgctggat cgtctgcatg aactgggcat cgaggaaatt     60

ttcggcgtac ctggtgacta taacctgcag ttcctggatc agatcatttc ccacaaagat    120

atgaaatggg ttggtaacgc gaacgagctg aatgcaagct acatggctga cggttatgca    180

cgcaccaaga aagctgcggc gttcctgact acttttggcg tcggcgagct gtctgcggta    240

aacggtctgg ccggctccta cgcggaaaac ctgccggtag tagaaatcgt cggttccccg    300

acctctaaag ttcagaacga gggtaaattc gtgcaccata ctctggccga tggtgacttc    360

aaacacttca tgaagatgca cgaaccggtc actgctgctc gtacgctgct gaccgcggaa    420

aatgcgactg tcgagattga tcgtgtactg agcgcactgc tgaaagaacg caagcctgta    480

tacatcaacc tgccggttga tgtcgcggcc gccaaagcgg aaaaaccatc tctgccgctg    540

aaaaaggaga acagcacctc taacaccagc gaccaggaaa tcctgaacaa gatccaggag    600

tctctgaaga acgctaaaaa gccgatcgta atcaccggcc atgagattat ctctttcggt    660

ctggagaaaa ctgtcaccca gttcatcagc aaaaccaaac tgccgatcac caccctgaac    720

ttcggtaaat cctccgttga cgaagcgctg ccgtcctttc tgggtattta caacggcact    780

ctgtctgagc cgaacctgaa agagttcgtg gagtctgcgg attttatcct gatgctgggc    840

gtgaaactga cggattcctc caccggtgca ttcacccacc acctgaatga gaataaaatg    900

atctctctga acattgatga gggcaaaatc ttcaacgagc gtattcagaa cttcgatttc    960

gaatccctga tctcctccct gctggatctg tccgagattg aatataaagg caaatacatt   1020

gataagaagc aagaggactt cgtaccgtct aacgcgctgc tgagccagga ccgtctgtgg   1080

caagctgtgg aaaacctgac ccagtccaac gaaaccatcg tggcggaaca gggtacctcc   1140

ttcttcggtg ctagctctat cttcctgaaa tctaaaagcc acttcatcgg tcagccactg   1200

tggggctcta ttggctacac cttcccggca gcgctgggtt cccaaatcgc agacaaagaa   1260

tcccgccacc tgctgttcat tggtgacggc tctctgcaac tgaccgtaca ggagctgggt   1320

ctggcgattc gtgagaaaat caacccgatt tgtttcatca tcaacaacga tggctacact   1380

gttgagcgtg agatccacgg cccgaaccag tcctacaacg acattccgat gtggaactac   1440

tctaaactgc cggaatcctt cggtgcgact gaagaccgtg tcgtaagcaa gatcgtccgt   1500

accgaaaacg aattcgtgtc tgtcatgaaa gaagcacagg cggacccgaa ccgcatgtac   1560

tggatcgagc tgattctggc taaagagggc gcgccaaaag tactgaaaaa gatgggtaaa   1620

ctgttcgcag aacagaacaa atcctaa                                       1647


<210>  45
<211>  3696
<212>  DNA
<213>  Mycobacterium tuberculosis


<220>
<221>  CDS
<222>  (1)..(3696)

<400>  45
gtg gcc aac ata agt tca cca ttc ggg caa aac gaa tgg ctg gtc gaa       48
Val Ala Asn Ile Ser Ser Pro Phe Gly Gln Asn Glu Trp Leu Val Glu         
1               5                   10                  15              

gag atg tac cgc aag ttc cgc gac gac ccc tcc tcg gtc gat ccc agc       96
Glu Met Tyr Arg Lys Phe Arg Asp Asp Pro Ser Ser Val Asp Pro Ser         
            20                  25                  30                  

tgg cac gag ttc ctg gtt gac tac agc ccc gaa ccc acc tcc caa cca      144
Trp His Glu Phe Leu Val Asp Tyr Ser Pro Glu Pro Thr Ser Gln Pro         
        35                  40                  45                      

gct gcc gaa cca acc cgg gtt acc tcg cca ctc gtt gcc gag cgg gcc      192
Ala Ala Glu Pro Thr Arg Val Thr Ser Pro Leu Val Ala Glu Arg Ala         
    50                  55                  60                          

gct gcg gcc gcc ccg cag gca ccc ccc aag ccg gcc gac acc gcg gcc      240
Ala Ala Ala Ala Pro Gln Ala Pro Pro Lys Pro Ala Asp Thr Ala Ala         
65                  70                  75                  80          

gcg ggc aac ggc gtg gtc gcc gca ctg gcc gcc aaa act gcc gtt ccc      288
Ala Gly Asn Gly Val Val Ala Ala Leu Ala Ala Lys Thr Ala Val Pro         
                85                  90                  95              

ccg cca gcc gaa ggt gac gag gta gcg gtg ctg cgc ggc gcc gcc gcg      336
Pro Pro Ala Glu Gly Asp Glu Val Ala Val Leu Arg Gly Ala Ala Ala         
            100                 105                 110                 

gcc gtc gtc aag aac atg tcc gcg tcg ttg gag gtg ccg acg gcg acc      384
Ala Val Val Lys Asn Met Ser Ala Ser Leu Glu Val Pro Thr Ala Thr         
        115                 120                 125                     

agc gtc cgg gcg gtc ccg gcc aag cta ctg atc gac aac cgg atc gtc      432
Ser Val Arg Ala Val Pro Ala Lys Leu Leu Ile Asp Asn Arg Ile Val         
    130                 135                 140                         

atc aac aac cag ttg aag cgg acc cgc ggc ggc aag atc tcg ttc acg      480
Ile Asn Asn Gln Leu Lys Arg Thr Arg Gly Gly Lys Ile Ser Phe Thr         
145                 150                 155                 160         

cat ttg ctg ggc tac gcc ctg gtg cag gcg gtg aag aaa ttc ccg aac      528
His Leu Leu Gly Tyr Ala Leu Val Gln Ala Val Lys Lys Phe Pro Asn         
                165                 170                 175             

atg aac cgg cac tac acc gaa gtc gac ggc aag ccc acc gcg gtc acg      576
Met Asn Arg His Tyr Thr Glu Val Asp Gly Lys Pro Thr Ala Val Thr         
            180                 185                 190                 

ccg gcg cac acc aat ctc ggc ctg gcg atc gac ctg caa ggc aag gac      624
Pro Ala His Thr Asn Leu Gly Leu Ala Ile Asp Leu Gln Gly Lys Asp         
        195                 200                 205                     

ggg aag cgt tcc ctg gtg gtg gcc ggc atc aag cgg tgc gag acc atg      672
Gly Lys Arg Ser Leu Val Val Ala Gly Ile Lys Arg Cys Glu Thr Met         
    210                 215                 220                         

cga ttc gcg cag ttc gtc acg gcc tac gaa gac atc gta cgc cgg gcc      720
Arg Phe Ala Gln Phe Val Thr Ala Tyr Glu Asp Ile Val Arg Arg Ala         
225                 230                 235                 240         

cgc gac ggc aag ctg acc act gaa gac ttt gcc ggc gtg acg att tcg      768
Arg Asp Gly Lys Leu Thr Thr Glu Asp Phe Ala Gly Val Thr Ile Ser         
                245                 250                 255             

ctg acc aat ccc gga acc atc ggc acc gtg cat tcg gtg ccg cgg ctg      816
Leu Thr Asn Pro Gly Thr Ile Gly Thr Val His Ser Val Pro Arg Leu         
            260                 265                 270                 

atg ccc ggc cag ggc gcc atc atc ggc gtg ggc gcc atg gaa tac ccc      864
Met Pro Gly Gln Gly Ala Ile Ile Gly Val Gly Ala Met Glu Tyr Pro         
        275                 280                 285                     

gcc gag ttt caa ggc gcc agc gag gaa cgc atc gcc gag ctg ggc atc      912
Ala Glu Phe Gln Gly Ala Ser Glu Glu Arg Ile Ala Glu Leu Gly Ile         
    290                 295                 300                         

ggc aaa ttg atc act ttg acc tcc acc tac gac cac cgc atc atc cag      960
Gly Lys Leu Ile Thr Leu Thr Ser Thr Tyr Asp His Arg Ile Ile Gln         
305                 310                 315                 320         

ggc gcg gaa tcg ggc gac ttc ctg cgc acc atc cac gag ttg ctg ctc     1008
Gly Ala Glu Ser Gly Asp Phe Leu Arg Thr Ile His Glu Leu Leu Leu         
                325                 330                 335             

tcg gat ggc ttc tgg gac gag gtc ttc cgc gaa ctg agc atc cca tat     1056
Ser Asp Gly Phe Trp Asp Glu Val Phe Arg Glu Leu Ser Ile Pro Tyr         
            340                 345                 350                 

ctg ccg gtg cgc tgg agc acc gac aac ccc gac tcg atc gtc gac aag     1104
Leu Pro Val Arg Trp Ser Thr Asp Asn Pro Asp Ser Ile Val Asp Lys         
        355                 360                 365                     

aac gct cgc gtc atg aac ttg atc gcg gcc tac cgc aac cgc ggc cat     1152
Asn Ala Arg Val Met Asn Leu Ile Ala Ala Tyr Arg Asn Arg Gly His         
    370                 375                 380                         

ctg atg gcc gat acc gac ccg ctg cgg ttg gac aaa gct cgg ttc cgc     1200
Leu Met Ala Asp Thr Asp Pro Leu Arg Leu Asp Lys Ala Arg Phe Arg         
385                 390                 395                 400         

agt cac ccc gac ctc gaa gtg ctg acc cac ggc ctg acg ctg tgg gat     1248
Ser His Pro Asp Leu Glu Val Leu Thr His Gly Leu Thr Leu Trp Asp         
                405                 410                 415             

ctc gat cgg gtg ttc aag gtc gac ggc ttt gcc ggt gcg cag tac aag     1296
Leu Asp Arg Val Phe Lys Val Asp Gly Phe Ala Gly Ala Gln Tyr Lys         
            420                 425                 430                 

aaa ctg cgc gac gtg ctg ggc ttg ctg cgc gat gcc tac tgc cgc cac     1344
Lys Leu Arg Asp Val Leu Gly Leu Leu Arg Asp Ala Tyr Cys Arg His         
        435                 440                 445                     

atc ggc gtg gag tac gcc cat atc ctc gac ccc gaa caa aag gag tgg     1392
Ile Gly Val Glu Tyr Ala His Ile Leu Asp Pro Glu Gln Lys Glu Trp         
    450                 455                 460                         

ctc gaa caa cgg gtc gag acc aag cac gtc aaa ccc act gtg gcc caa     1440
Leu Glu Gln Arg Val Glu Thr Lys His Val Lys Pro Thr Val Ala Gln         
465                 470                 475                 480         

cag aaa tac atc ctc agc aag ctc aac gcc gcc gag gcc ttt gaa acg     1488
Gln Lys Tyr Ile Leu Ser Lys Leu Asn Ala Ala Glu Ala Phe Glu Thr         
                485                 490                 495             

ttc cta cag acc aag tac gtc ggc cag aag cgg ttc tcg ctg gaa ggc     1536
Phe Leu Gln Thr Lys Tyr Val Gly Gln Lys Arg Phe Ser Leu Glu Gly         
            500                 505                 510                 

gcc gaa agc gtg atc ccg atg atg gac gcg gcg atc gac cag tgc gct     1584
Ala Glu Ser Val Ile Pro Met Met Asp Ala Ala Ile Asp Gln Cys Ala         
        515                 520                 525                     

gag cac ggc ctc gac gag gtg gtc atc ggg atg ccg cac cgg ggc cgg     1632
Glu His Gly Leu Asp Glu Val Val Ile Gly Met Pro His Arg Gly Arg         
    530                 535                 540                         

ctc aac gtg ctg gcc aac atc gtc ggc aag ccg tac tcg cag atc ttc     1680
Leu Asn Val Leu Ala Asn Ile Val Gly Lys Pro Tyr Ser Gln Ile Phe         
545                 550                 555                 560         

acc gag ttc gag ggc aac ctg aat ccg tcg cag gcg cac ggc tcc ggt     1728
Thr Glu Phe Glu Gly Asn Leu Asn Pro Ser Gln Ala His Gly Ser Gly         
                565                 570                 575             

gac gtc aag tac cac ctg ggc gcc acc ggg ctg tac ctg cag atg ttc     1776
Asp Val Lys Tyr His Leu Gly Ala Thr Gly Leu Tyr Leu Gln Met Phe         
            580                 585                 590                 

ggc gac aac gac att cag gtg tcg ctg acc gcc aac ccg tcg cat ctg     1824
Gly Asp Asn Asp Ile Gln Val Ser Leu Thr Ala Asn Pro Ser His Leu         
        595                 600                 605                     

gag gcc gtc gac ccg gtg ctg gag gga ttg gtg cgg gcc aag cag gat     1872
Glu Ala Val Asp Pro Val Leu Glu Gly Leu Val Arg Ala Lys Gln Asp         
    610                 615                 620                         

ctg ctc gac cac gga agc atc gac agc gac ggc caa cgg gcg ttc tcg     1920
Leu Leu Asp His Gly Ser Ile Asp Ser Asp Gly Gln Arg Ala Phe Ser         
625                 630                 635                 640         

gtg gtg ccg ctg atg ttg cat ggc gat gcc gcg ttc gcc ggt cag ggt     1968
Val Val Pro Leu Met Leu His Gly Asp Ala Ala Phe Ala Gly Gln Gly         
                645                 650                 655             

gtg gtc gcc gag acg ctg aac ctg gcg aat ctg ccg ggc tac cgc gtc     2016
Val Val Ala Glu Thr Leu Asn Leu Ala Asn Leu Pro Gly Tyr Arg Val         
            660                 665                 670                 

ggc ggc acc atc cac atc atc gtc aac aac cag atc ggc ttc acc acc     2064
Gly Gly Thr Ile His Ile Ile Val Asn Asn Gln Ile Gly Phe Thr Thr         
        675                 680                 685                     

gcg ccc gag tat tcc agg tcc agc gag tac tgc acc gac gtc gca aag     2112
Ala Pro Glu Tyr Ser Arg Ser Ser Glu Tyr Cys Thr Asp Val Ala Lys         
    690                 695                 700                         

atg atc ggg gca ccg atc ttt cac gtc aac ggc gac gac ccg gag gcg     2160
Met Ile Gly Ala Pro Ile Phe His Val Asn Gly Asp Asp Pro Glu Ala         
705                 710                 715                 720         

tgt gtc tgg gtg gcg cgg ttg gcg gtg gac ttc cga caa cgg ttc aag     2208
Cys Val Trp Val Ala Arg Leu Ala Val Asp Phe Arg Gln Arg Phe Lys         
                725                 730                 735             

aag gac gtc gtc atc gac atg ctg tgc tac cgc cgc cgc ggg cac aac     2256
Lys Asp Val Val Ile Asp Met Leu Cys Tyr Arg Arg Arg Gly His Asn         
            740                 745                 750                 

gag ggt gac gac ccg tcg atg acc aac ccc tac gtg tac gac gtc gtc     2304
Glu Gly Asp Asp Pro Ser Met Thr Asn Pro Tyr Val Tyr Asp Val Val         
        755                 760                 765                     

gac acc aag cgc ggg gcc cgc aaa agc tac acc gaa gcc ctg atc gga     2352
Asp Thr Lys Arg Gly Ala Arg Lys Ser Tyr Thr Glu Ala Leu Ile Gly         
    770                 775                 780                         

cgt ggc gac atc tcg atg aag gag gcc gag gac gcg ctg cgc gac tac     2400
Arg Gly Asp Ile Ser Met Lys Glu Ala Glu Asp Ala Leu Arg Asp Tyr         
785                 790                 795                 800         

cag ggc cag ctg gaa cgg gtg ttc aac gaa gtg cgc gag ctg gag aag     2448
Gln Gly Gln Leu Glu Arg Val Phe Asn Glu Val Arg Glu Leu Glu Lys         
                805                 810                 815             

cac ggt gtg cag ccg agc gag tcg gtc gag tcc gac cag atg att ccc     2496
His Gly Val Gln Pro Ser Glu Ser Val Glu Ser Asp Gln Met Ile Pro         
            820                 825                 830                 

gcg ggg ctg gcc act gcg gtg gac aag tcg ctg ctg gcc cgg atc ggc     2544
Ala Gly Leu Ala Thr Ala Val Asp Lys Ser Leu Leu Ala Arg Ile Gly         
        835                 840                 845                     

gat gcg ttc ctc gcc ttg ccg aac ggc ttc acc gcg cac ccg cga gtc     2592
Asp Ala Phe Leu Ala Leu Pro Asn Gly Phe Thr Ala His Pro Arg Val         
    850                 855                 860                         

caa ccg gtg ctg gag aag cgc cgg gag atg gcc tat gaa ggc aag atc     2640
Gln Pro Val Leu Glu Lys Arg Arg Glu Met Ala Tyr Glu Gly Lys Ile         
865                 870                 875                 880         

gac tgg gcc ttt ggc gag ctg ctg gcg ctg ggc tcg ctg gtg gcc gaa     2688
Asp Trp Ala Phe Gly Glu Leu Leu Ala Leu Gly Ser Leu Val Ala Glu         
                885                 890                 895             

ggc aag ctg gtg cgc ttg tcg ggg cag gac agc cgc cgc ggc acc ttc     2736
Gly Lys Leu Val Arg Leu Ser Gly Gln Asp Ser Arg Arg Gly Thr Phe         
            900                 905                 910                 

tcc cag cgg cat tcg gtt ctc atc gac cgc cac act ggc gag gag ttc     2784
Ser Gln Arg His Ser Val Leu Ile Asp Arg His Thr Gly Glu Glu Phe         
        915                 920                 925                     

aca cca ctg cag ctg ctg gcg acc aac tcc gac ggc agc ccg acc ggc     2832
Thr Pro Leu Gln Leu Leu Ala Thr Asn Ser Asp Gly Ser Pro Thr Gly         
    930                 935                 940                         

gga aag ttc ctg gtc tac gac tcg cca ctg tcg gag tac gcc gcc gtc     2880
Gly Lys Phe Leu Val Tyr Asp Ser Pro Leu Ser Glu Tyr Ala Ala Val         
945                 950                 955                 960         

ggc ttc gag tac ggc tac act gtg ggc aat ccg gac gcc gtg gtg ctc     2928
Gly Phe Glu Tyr Gly Tyr Thr Val Gly Asn Pro Asp Ala Val Val Leu         
                965                 970                 975             

tgg gag gcg cag ttc ggc gac ttc gtc aac ggc gcg cag tcg atc atc     2976
Trp Glu Ala Gln Phe Gly Asp Phe Val Asn Gly Ala Gln Ser Ile Ile         
            980                 985                 990                 

gac gag ttc atc agc tcc ggt gag  gcc aag tgg ggc caa  ttg tcc aac   3024
Asp Glu Phe Ile Ser Ser Gly Glu  Ala Lys Trp Gly Gln  Leu Ser Asn       
        995                 1000                 1005                   

gtc gtg  ctg ctg tta ccg cac  ggg cac gag ggg cag  gga ccc gac      3069
Val Val  Leu Leu Leu Pro His  Gly His Glu Gly Gln  Gly Pro Asp          
    1010                 1015                 1020                      

cac act  tct gcc cgg atc gaa  cgc ttc ttg cag ttg  tgg gcg gaa      3114
His Thr  Ser Ala Arg Ile Glu  Arg Phe Leu Gln Leu  Trp Ala Glu          
    1025                 1030                 1035                      

ggt tcg  atg acc atc gcg atg  ccg tcg act ccg tcg  aac tac ttc      3159
Gly Ser  Met Thr Ile Ala Met  Pro Ser Thr Pro Ser  Asn Tyr Phe          
    1040                 1045                 1050                      

cac ctg  cta cgc cgg cat gcc  ctg gac ggc atc caa  cgc ccg ctg      3204
His Leu  Leu Arg Arg His Ala  Leu Asp Gly Ile Gln  Arg Pro Leu          
    1055                 1060                 1065                      

atc gtg  ttc acg ccc aag tcg  atg ttg cgt cac aag  gcc gcc gtc      3249
Ile Val  Phe Thr Pro Lys Ser  Met Leu Arg His Lys  Ala Ala Val          
    1070                 1075                 1080                      

agc gaa  atc aag gac ttc acc  gag atc aag ttc cgc  tca gtg ctg      3294
Ser Glu  Ile Lys Asp Phe Thr  Glu Ile Lys Phe Arg  Ser Val Leu          
    1085                 1090                 1095                      

gag gaa  ccc acc tat gag gac  ggc atc gga gac cgc  aac aag gtc      3339
Glu Glu  Pro Thr Tyr Glu Asp  Gly Ile Gly Asp Arg  Asn Lys Val          
    1100                 1105                 1110                      

agc cgg  atc ctg ctg acc agt  ggc aag ctg tat tac  gag ctg gcc      3384
Ser Arg  Ile Leu Leu Thr Ser  Gly Lys Leu Tyr Tyr  Glu Leu Ala          
    1115                 1120                 1125                      

gcc cgc  aag gcc aag gac aac  cgc aat gac ctc gcg  atc gtg cgg      3429
Ala Arg  Lys Ala Lys Asp Asn  Arg Asn Asp Leu Ala  Ile Val Arg          
    1130                 1135                 1140                      

ctt gaa  cag ctc gcc ccg ctg  ccc agg cgt cga ctg  cgt gaa acg      3474
Leu Glu  Gln Leu Ala Pro Leu  Pro Arg Arg Arg Leu  Arg Glu Thr          
    1145                 1150                 1155                      

ctg gac  cgc tac gag aac gtc  aag gag ttc ttc tgg  gtc caa gag      3519
Leu Asp  Arg Tyr Glu Asn Val  Lys Glu Phe Phe Trp  Val Gln Glu          
    1160                 1165                 1170                      

gaa ccg  gcc aac cag ggt gcg  tgg ccg cga ttc ggg  ctc gaa cta      3564
Glu Pro  Ala Asn Gln Gly Ala  Trp Pro Arg Phe Gly  Leu Glu Leu          
    1175                 1180                 1185                      

ccc gag  ctg ctg cct gac aag  ttg gcc ggg atc aag  cga atc tcg      3609
Pro Glu  Leu Leu Pro Asp Lys  Leu Ala Gly Ile Lys  Arg Ile Ser          
    1190                 1195                 1200                      

cgc cgg  gcg atg tca gcc ccg  tcg tca ggc tcg tcg  aag gtg cac      3654
Arg Arg  Ala Met Ser Ala Pro  Ser Ser Gly Ser Ser  Lys Val His          
    1205                 1210                 1215                      

gcc gtc  gaa cag cag gag atc  ctc gac gag gcg ttc  ggc tga          3696
Ala Val  Glu Gln Gln Glu Ile  Leu Asp Glu Ala Phe  Gly                  
    1220                 1225                 1230                      


<210>  46
<211>  1231
<212>  PRT
<213>  Mycobacterium tuberculosis

<400>  46

Val Ala Asn Ile Ser Ser Pro Phe Gly Gln Asn Glu Trp Leu Val Glu 
1               5                   10                  15      


Glu Met Tyr Arg Lys Phe Arg Asp Asp Pro Ser Ser Val Asp Pro Ser 
            20                  25                  30          


Trp His Glu Phe Leu Val Asp Tyr Ser Pro Glu Pro Thr Ser Gln Pro 
        35                  40                  45              


Ala Ala Glu Pro Thr Arg Val Thr Ser Pro Leu Val Ala Glu Arg Ala 
    50                  55                  60                  


Ala Ala Ala Ala Pro Gln Ala Pro Pro Lys Pro Ala Asp Thr Ala Ala 
65                  70                  75                  80  


Ala Gly Asn Gly Val Val Ala Ala Leu Ala Ala Lys Thr Ala Val Pro 
                85                  90                  95      


Pro Pro Ala Glu Gly Asp Glu Val Ala Val Leu Arg Gly Ala Ala Ala 
            100                 105                 110         


Ala Val Val Lys Asn Met Ser Ala Ser Leu Glu Val Pro Thr Ala Thr 
        115                 120                 125             


Ser Val Arg Ala Val Pro Ala Lys Leu Leu Ile Asp Asn Arg Ile Val 
    130                 135                 140                 


Ile Asn Asn Gln Leu Lys Arg Thr Arg Gly Gly Lys Ile Ser Phe Thr 
145                 150                 155                 160 


His Leu Leu Gly Tyr Ala Leu Val Gln Ala Val Lys Lys Phe Pro Asn 
                165                 170                 175     


Met Asn Arg His Tyr Thr Glu Val Asp Gly Lys Pro Thr Ala Val Thr 
            180                 185                 190         


Pro Ala His Thr Asn Leu Gly Leu Ala Ile Asp Leu Gln Gly Lys Asp 
        195                 200                 205             


Gly Lys Arg Ser Leu Val Val Ala Gly Ile Lys Arg Cys Glu Thr Met 
    210                 215                 220                 


Arg Phe Ala Gln Phe Val Thr Ala Tyr Glu Asp Ile Val Arg Arg Ala 
225                 230                 235                 240 


Arg Asp Gly Lys Leu Thr Thr Glu Asp Phe Ala Gly Val Thr Ile Ser 
                245                 250                 255     


Leu Thr Asn Pro Gly Thr Ile Gly Thr Val His Ser Val Pro Arg Leu 
            260                 265                 270         


Met Pro Gly Gln Gly Ala Ile Ile Gly Val Gly Ala Met Glu Tyr Pro 
        275                 280                 285             


Ala Glu Phe Gln Gly Ala Ser Glu Glu Arg Ile Ala Glu Leu Gly Ile 
    290                 295                 300                 


Gly Lys Leu Ile Thr Leu Thr Ser Thr Tyr Asp His Arg Ile Ile Gln 
305                 310                 315                 320 


Gly Ala Glu Ser Gly Asp Phe Leu Arg Thr Ile His Glu Leu Leu Leu 
                325                 330                 335     


Ser Asp Gly Phe Trp Asp Glu Val Phe Arg Glu Leu Ser Ile Pro Tyr 
            340                 345                 350         


Leu Pro Val Arg Trp Ser Thr Asp Asn Pro Asp Ser Ile Val Asp Lys 
        355                 360                 365             


Asn Ala Arg Val Met Asn Leu Ile Ala Ala Tyr Arg Asn Arg Gly His 
    370                 375                 380                 


Leu Met Ala Asp Thr Asp Pro Leu Arg Leu Asp Lys Ala Arg Phe Arg 
385                 390                 395                 400 


Ser His Pro Asp Leu Glu Val Leu Thr His Gly Leu Thr Leu Trp Asp 
                405                 410                 415     


Leu Asp Arg Val Phe Lys Val Asp Gly Phe Ala Gly Ala Gln Tyr Lys 
            420                 425                 430         


Lys Leu Arg Asp Val Leu Gly Leu Leu Arg Asp Ala Tyr Cys Arg His 
        435                 440                 445             


Ile Gly Val Glu Tyr Ala His Ile Leu Asp Pro Glu Gln Lys Glu Trp 
    450                 455                 460                 


Leu Glu Gln Arg Val Glu Thr Lys His Val Lys Pro Thr Val Ala Gln 
465                 470                 475                 480 


Gln Lys Tyr Ile Leu Ser Lys Leu Asn Ala Ala Glu Ala Phe Glu Thr 
                485                 490                 495     


Phe Leu Gln Thr Lys Tyr Val Gly Gln Lys Arg Phe Ser Leu Glu Gly 
            500                 505                 510         


Ala Glu Ser Val Ile Pro Met Met Asp Ala Ala Ile Asp Gln Cys Ala 
        515                 520                 525             


Glu His Gly Leu Asp Glu Val Val Ile Gly Met Pro His Arg Gly Arg 
    530                 535                 540                 


Leu Asn Val Leu Ala Asn Ile Val Gly Lys Pro Tyr Ser Gln Ile Phe 
545                 550                 555                 560 


Thr Glu Phe Glu Gly Asn Leu Asn Pro Ser Gln Ala His Gly Ser Gly 
                565                 570                 575     


Asp Val Lys Tyr His Leu Gly Ala Thr Gly Leu Tyr Leu Gln Met Phe 
            580                 585                 590         


Gly Asp Asn Asp Ile Gln Val Ser Leu Thr Ala Asn Pro Ser His Leu 
        595                 600                 605             


Glu Ala Val Asp Pro Val Leu Glu Gly Leu Val Arg Ala Lys Gln Asp 
    610                 615                 620                 


Leu Leu Asp His Gly Ser Ile Asp Ser Asp Gly Gln Arg Ala Phe Ser 
625                 630                 635                 640 


Val Val Pro Leu Met Leu His Gly Asp Ala Ala Phe Ala Gly Gln Gly 
                645                 650                 655     


Val Val Ala Glu Thr Leu Asn Leu Ala Asn Leu Pro Gly Tyr Arg Val 
            660                 665                 670         


Gly Gly Thr Ile His Ile Ile Val Asn Asn Gln Ile Gly Phe Thr Thr 
        675                 680                 685             


Ala Pro Glu Tyr Ser Arg Ser Ser Glu Tyr Cys Thr Asp Val Ala Lys 
    690                 695                 700                 


Met Ile Gly Ala Pro Ile Phe His Val Asn Gly Asp Asp Pro Glu Ala 
705                 710                 715                 720 


Cys Val Trp Val Ala Arg Leu Ala Val Asp Phe Arg Gln Arg Phe Lys 
                725                 730                 735     


Lys Asp Val Val Ile Asp Met Leu Cys Tyr Arg Arg Arg Gly His Asn 
            740                 745                 750         


Glu Gly Asp Asp Pro Ser Met Thr Asn Pro Tyr Val Tyr Asp Val Val 
        755                 760                 765             


Asp Thr Lys Arg Gly Ala Arg Lys Ser Tyr Thr Glu Ala Leu Ile Gly 
    770                 775                 780                 


Arg Gly Asp Ile Ser Met Lys Glu Ala Glu Asp Ala Leu Arg Asp Tyr 
785                 790                 795                 800 


Gln Gly Gln Leu Glu Arg Val Phe Asn Glu Val Arg Glu Leu Glu Lys 
                805                 810                 815     


His Gly Val Gln Pro Ser Glu Ser Val Glu Ser Asp Gln Met Ile Pro 
            820                 825                 830         


Ala Gly Leu Ala Thr Ala Val Asp Lys Ser Leu Leu Ala Arg Ile Gly 
        835                 840                 845             


Asp Ala Phe Leu Ala Leu Pro Asn Gly Phe Thr Ala His Pro Arg Val 
    850                 855                 860                 


Gln Pro Val Leu Glu Lys Arg Arg Glu Met Ala Tyr Glu Gly Lys Ile 
865                 870                 875                 880 


Asp Trp Ala Phe Gly Glu Leu Leu Ala Leu Gly Ser Leu Val Ala Glu 
                885                 890                 895     


Gly Lys Leu Val Arg Leu Ser Gly Gln Asp Ser Arg Arg Gly Thr Phe 
            900                 905                 910         


Ser Gln Arg His Ser Val Leu Ile Asp Arg His Thr Gly Glu Glu Phe 
        915                 920                 925             


Thr Pro Leu Gln Leu Leu Ala Thr Asn Ser Asp Gly Ser Pro Thr Gly 
    930                 935                 940                 


Gly Lys Phe Leu Val Tyr Asp Ser Pro Leu Ser Glu Tyr Ala Ala Val 
945                 950                 955                 960 


Gly Phe Glu Tyr Gly Tyr Thr Val Gly Asn Pro Asp Ala Val Val Leu 
                965                 970                 975     


Trp Glu Ala Gln Phe Gly Asp Phe Val Asn Gly Ala Gln Ser Ile Ile 
            980                 985                 990         


Asp Glu Phe Ile Ser Ser Gly Glu  Ala Lys Trp Gly Gln  Leu Ser Asn 
        995                 1000                 1005             


Val Val  Leu Leu Leu Pro His  Gly His Glu Gly Gln  Gly Pro Asp 
    1010                 1015                 1020             


His Thr  Ser Ala Arg Ile Glu  Arg Phe Leu Gln Leu  Trp Ala Glu 
    1025                 1030                 1035             


Gly Ser  Met Thr Ile Ala Met  Pro Ser Thr Pro Ser  Asn Tyr Phe 
    1040                 1045                 1050             


His Leu  Leu Arg Arg His Ala  Leu Asp Gly Ile Gln  Arg Pro Leu 
    1055                 1060                 1065             


Ile Val  Phe Thr Pro Lys Ser  Met Leu Arg His Lys  Ala Ala Val 
    1070                 1075                 1080             


Ser Glu  Ile Lys Asp Phe Thr  Glu Ile Lys Phe Arg  Ser Val Leu 
    1085                 1090                 1095             


Glu Glu  Pro Thr Tyr Glu Asp  Gly Ile Gly Asp Arg  Asn Lys Val 
    1100                 1105                 1110             


Ser Arg  Ile Leu Leu Thr Ser  Gly Lys Leu Tyr Tyr  Glu Leu Ala 
    1115                 1120                 1125             


Ala Arg  Lys Ala Lys Asp Asn  Arg Asn Asp Leu Ala  Ile Val Arg 
    1130                 1135                 1140             


Leu Glu  Gln Leu Ala Pro Leu  Pro Arg Arg Arg Leu  Arg Glu Thr 
    1145                 1150                 1155             


Leu Asp  Arg Tyr Glu Asn Val  Lys Glu Phe Phe Trp  Val Gln Glu 
    1160                 1165                 1170             


Glu Pro  Ala Asn Gln Gly Ala  Trp Pro Arg Phe Gly  Leu Glu Leu 
    1175                 1180                 1185             


Pro Glu  Leu Leu Pro Asp Lys  Leu Ala Gly Ile Lys  Arg Ile Ser 
    1190                 1195                 1200             


Arg Arg  Ala Met Ser Ala Pro  Ser Ser Gly Ser Ser  Lys Val His 
    1205                 1210                 1215             


Ala Val  Glu Gln Gln Glu Ile  Leu Asp Glu Ala Phe  Gly 
    1220                 1225                 1230     


<210>  47
<211>  3696
<212>  DNA
<213>  Artificial

<220>
<223>  Mycobacterium tuberculosis  -ketoglutarate decarboxylase Kgd 
       codon optimised gene

<400>  47
atggctaata tctcctctcc gtttggtcag aatgaatggc tggtagaaga aatgtaccgt     60

aaattccgcg atgacccgtc ctctgtggac ccgtcctggc atgaattcct ggtagactac    120

agcccggagc cgaccagcca accggcagcg gaaccaaccc gcgttacttc tccgctggta    180

gcggaacgtg cagctgctgc cgcgcctcag gcgccgccta aaccggcgga tactgccgca    240

gccggtaacg gtgtggtggc cgcactggct gctaagactg cggttccgcc gccagcagaa    300

ggcgatgaag ttgcagtcct gcgcggtgcg gcggctgcag tggtgaaaaa catgagcgcg    360

tccctggagg taccgaccgc cacgagcgtg cgcgcggtcc ctgctaaact gctgattgat    420

aaccgtattg tgatcaacaa ccagctgaaa cgtacccgtg gtggcaagat ctccttcact    480

catctgctgg gttatgcact ggtacaagcg gttaagaaat tccctaacat gaaccgtcat    540

tacactgagg tcgacggtaa accgacggct gttactccgg cacacacgaa cctgggcctg    600

gcgatcgacc tgcaaggtaa agatggtaag cgctccctgg tagttgcggg tattaaacgt    660

tgcgaaacca tgcgtttcgc acaattcgta accgcctacg aggacattgt ccgccgtgct    720

cgtgatggca aactgaccac cgaagatttt gcgggcgtta ctattagcct gaccaaccca    780

ggcaccatcg gcaccgtgca cagcgtacct cgtctgatgc cgggccaagg tgcgattatc    840

ggtgtgggtg ccatggagta cccggcagaa tttcagggtg cttctgaaga gcgcatcgcc    900

gagctgggta ttggtaaact gatcaccctg acttctacct atgaccaccg catcattcag    960

ggcgcagaat ccggtgactt cctgcgcact attcacgaac tgctgctgtc cgacggtttc   1020

tgggatgaag tttttcgtga actgagcatc ccatatctgc cagttcgctg gtccaccgac   1080

aatccggact ctatcgttga caaaaacgct cgcgtaatga acctgatcgc tgcttatcgt   1140

aatcgtggtc acctgatggc tgatacggat ccgctgcgcc tggataaagc tcgtttccgt   1200

tcccacccgg acctggaagt gctgacccat ggtctgactc tgtgggatct ggaccgcgtg   1260

ttcaaagtag atggtttcgc gggtgctcag tacaagaagc tgcgtgacgt gctgggtctg   1320

ctgcgtgatg cgtactgtcg tcacattggt gtggagtacg cccacattct ggatccggaa   1380

cagaaagaat ggctggagca gcgtgtcgag accaaacacg taaaaccgac cgtagcgcag   1440

cagaaatata tcctgtccaa actgaacgcc gccgaggctt tcgaaacttt cctgcagacc   1500

aagtacgtgg gccagaaacg cttcagcctg gagggtgcgg aaagcgttat tccgatgatg   1560

gatgcagcta tcgatcagtg cgcggaacat ggtctggatg aagtcgttat cggtatgccg   1620

caccgtggtc gcctgaacgt actggcaaac atcgtcggta aaccatattc tcagatcttc   1680

acggaattcg agggcaacct gaacccgtcc caagcccacg gctccggcga cgtaaaatat   1740

catctgggtg ctaccggcct gtatctgcag atgttcggtg ataacgacat ccaggtatct   1800

ctgactgcta acccgagcca cctggaggcg gttgatcctg ttctggaagg tctggttcgc   1860

gccaaacagg atctgctgga ccacggctct atcgacagcg atggccagcg tgcattcagc   1920

gttgtaccgc tgatgctgca tggcgacgcg gcgttcgccg gtcagggtgt cgtagcagaa   1980

actctgaacc tggcgaacct gcctggctat cgcgtgggtg gcaccattca catcatcgtt   2040

aacaaccaaa tcggtttcac cacggcaccg gagtatagcc gttctagcga atattgcacc   2100

gacgtagcca aaatgatcgg tgcgccgatc ttccatgtaa acggtgacga tccagaggcc   2160

tgcgtgtggg tggctcgtct ggccgtagac ttccgccagc gttttaagaa agatgtggtt   2220

atcgacatgc tgtgctaccg ccgtcgtggt cacaacgaag gtgatgatcc gtctatgact   2280

aacccgtatg tctatgacgt ggtggacacc aagcgtggtg cacgcaaatc ttacacggag   2340

gccctgatcg gtcgtggcga catctctatg aaagaagcgg aagacgctct gcgtgattac   2400

cagggtcagc tggaacgtgt gttcaatgag gtgcgtgagc tggaaaagca cggcgtacaa   2460

ccgtccgaat ccgtagagtc cgatcagatg atccctgctg gtctggcaac tgctgttgat   2520

aaaagcctgc tggcgcgtat cggcgacgca ttcctggcgc tgccgaatgg ctttaccgcg   2580

cacccgcgcg tacagccggt actggaaaaa cgtcgtgaaa tggcctacga aggtaaaatc   2640

gattgggcct tcggtgagct gctggccctg ggctctctgg tggctgaggg caagctggta   2700

cgcctgagcg gccaggactc ccgtcgcggc actttttctc agcgtcacag cgtcctgatc   2760

gatcgtcaca ccggcgaaga attcacgccg ctgcaactgc tggctactaa ctccgatggt   2820

agcccgaccg gtggtaagtt cctggtgtac gattccccgc tgtccgaata tgctgcagtt   2880

ggtttcgagt atggttacac cgttggcaac ccggacgcag tggttctgtg ggaagcgcag   2940

ttcggcgatt tcgttaacgg tgcccagtcc attatcgatg agtttattag cagcggcgag   3000

gccaaatggg gccagctgtc taacgttgtg ctgctgctgc ctcacggcca cgagggtcaa   3060

ggcccggacc acacctccgc ccgtatcgaa cgcttcctgc agctgtgggc tgaaggctct   3120

atgaccatcg cgatgccgtc taccccaagc aactacttcc acctgctgcg tcgccacgca   3180

ctggacggca ttcagcgccc gctgatcgtt ttcaccccaa aatccatgct gcgccacaaa   3240

gcagctgttt ctgaaatcaa agattttacg gaaattaaat tccgttctgt gctggaagaa   3300

ccaacctacg aagacggtat tggcgaccgc aacaaggtaa gccgtatcct gctgacctcc   3360

ggcaaactgt actacgagct ggcagcacgt aaggcaaaag ataaccgcaa cgacctggcc   3420

atcgtccgcc tggaacagct ggcgccactg ccacgccgtc gcctgcgtga aaccctggat   3480

cgctacgaaa acgtaaaaga attcttctgg gtgcaggaag aaccggcaaa ccagggtgcg   3540

tggccgcgct ttggtctgga actgccggaa ctgctgccgg ataaactggc aggtatcaag   3600

cgcatcagcc gtcgcgctat gagcgccccg tcttctggta gctctaaagt acacgctgta   3660

gaacagcaag agatcctgga tgaggccttc ggctaa                             3696


<210>  48
<211>  74
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for amplification of Bacillus subtilis 
       aminotransferase x

<400>  48
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgaagg ttttagtcaa     60

tggccggctg attg                                                       74


<210>  49
<211>  62
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for amplification of Bacillus subtilis 
       aminotransferase x

<400>  49
ggggaccact ttgtacaaga aagctgggtt tatgaaatgc tagcagcctg ttgaatgctt     60

tc                                                                    62


<210>  50
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for amplification of Bacillus subtilis 
       aminotransferase y

<400>  50
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgactc atgatttgat     60

agaaaaaagt aaaaagcacc tc                                              82


<210>  51
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for amplification of Bacillus subtilis 
       aminotransferase y

<400>  51
ggggaccact ttgtacaaga aagctgggtt caatcttcaa ggctcgtaac ctcgtgg        57


<210>  52
<211>  64
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for amplification of Rhodobacter sphaeroides 
       aminotransferase

<400>  52
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgcccg gttgcggggg     60

cttg                                                                  64


<210>  53
<211>  51
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for amplification of Rhodobacter sphaeroides 
       aminotransferase

<400>  53
ggggaccact ttgtacaaga aagctgggtt cagacggcgg ccggttcttt c              51


<210>  54
<211>  78
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for amplification of Legionella pneumophila 
       aminotransferase

<400>  54
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgagta tcgcatttgt     60

taacggcaag tattgttg                                                   78


<210>  55
<211>  67
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for amplification of Legionella pneumophila 
       aminotransferase

<400>  55
ggggaccact ttgtacaaga aagctgggtt tagtttacta gttgttggta ggaatcatta     60

attatcc                                                               67


<210>  56
<211>  76
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for amplification of Nitrosomonas europaea 
       aminotransferase

<400>  56
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgattt acctcaatgg     60

caaatttctg ccgatg                                                     76


<210>  57
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for amplification of Nitrosomonas europaea 
       aminotransferase

<400>  57
ggggaccact ttgtacaaga aagctgggtt tactggcgtg gagcatgccc                50


<210>  58
<211>  79
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for amplification of Neisseria gonorrhoeae 
       aminotransferase

<400>  58
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgagga taaatatgaa     60

ccgtaacgaa attttattc                                                  79


<210>  59
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for amplification of Neisseria gonorrhoeae 
       aminotransferase

<400>  59
ggggaccact ttgtacaaga aagctgggtt catgcagcca tcgccttgaa cacttc         56


<210>  60
<211>  66
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for amplification of Pseudomonas aeruginosa 
       aminotransferase

<400>  60
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgtcga tggccgatcg     60

tgatgg                                                                66


<210>  61
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for amplification of Pseudomonas aeruginosa 
       aminotransferase

<400>  61
ggggaccact ttgtacaaga aagctgggtt tacttgacca gggtacgcca ctc            53


<210>  62
<211>  67
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for amplification of Rhodopseudomonas palustris 
       aminotransferase

<400>  62
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgaagc tgataccgtg     60

ccgcgcc                                                               67


<210>  63
<211>  51
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for amplification of Rhodopseudomonas palustris 
       aminotransferase

<400>  63
ggggaccact ttgtacaaga aagctgggtt caggcgaccg cgcggatcac c              51


<210>  64
<211>  1353
<212>  DNA
<213>  Bacillus subtilis aminotransferase gene (gi16077991)

<400>  64
atggagatga tggggatgga aaacattcag caaaatcagg gattaaagca aaaagatgag     60

caatttgtgt ggcatgccat gaagggagcg catcaagcgg acagcctgat agcccagaag    120

gccgaagggg cctgggtaac cgacacagac ggacgccgct atttggatgc gatgtccggt    180

ttgtggtgcg tcaacattgg ttacggcaga aaggagcttg cggaggctgc ctatgagcaa    240

ctaaaggagc tgccttacta cccgttaacg caaagtcacg cacccgcaat tcaactggcg    300

gaaaagctga atgaatggct tggcggcgat tatgttattt ttttttccaa cagcggatcg    360

gaagcaaacg aaactgcttt taaaattgcc cgccagtacc atctgcaaaa cggcgaccac    420

agccgttata aattcatctc aagatatcgg gcataccacg gcaatacatt gggagcgctc    480

tcagctaccg gacaggcgca gcggaaatat aaatacgagc ctttgagcca agggttcctg    540

catgcagctc cgccagatat ataccggaat cctgatgatg cagacacgct tgaaagcgca    600

aatgaaatcg accgcatcat gacatgggaa ttaagcgaaa cgattgccgg ggtcattatg    660

gagcccatca ttacaggcgg aggcatccta atgccgccgg acggatatat gaagaaggtg    720

gaggacattt gccggcgcca cggagccctt ttgatttgcg atgaagtgat ctgcgggttt    780

ggacggacag gtgagccgtt cgggtttatg cactacggtg tgaagcctga tatcattacg    840

atggcaaagg gaatcacaag cgcgtatctg ccattgtcag cgactgctgt gaaacgggac    900

attttcgaag cgtatcaggg ggaagctcct tatgaccgtt tccgccacgt gaacacgttc    960

ggcggaagcc cggctgcctg tgctttggcg ttgaaaaacc tgcaaattat ggaggacgaa   1020

cagctgattc agcgatcccg tgatcttgga gcaaagcttt taggtgagct tcaagctctg   1080

agagaacacc cggcagtcgg ggatgttaga ggaaaagggc tgctgatcgg aatcgaactc   1140

gtcaaagaca aattgactaa agagccggct gatgccgcca aagtaaacca agtggttgcg   1200

gcgtgcaaag aaaaagggct gatcatcggc aaaaacggcg atacagtcgc cggctacaac   1260

aatgtcatcc acgttgcgcc gccattttgc ctgacagaag aggacctttc ctttatcgtg   1320

aaaacggtga aagaaagctt tcaaacgata taa                                1353


<210>  65
<211>  450
<212>  PRT
<213>  amino acid sequence of B. subtilis aminotransferase (gi16077991)

<400>  65

Met Glu Met Met Gly Met Glu Asn Ile Gln Gln Asn Gln Gly Leu Lys 
1               5                   10                  15      


Gln Lys Asp Glu Gln Phe Val Trp His Ala Met Lys Gly Ala His Gln 
            20                  25                  30          


Ala Asp Ser Leu Ile Ala Gln Lys Ala Glu Gly Ala Trp Val Thr Asp 
        35                  40                  45              


Thr Asp Gly Arg Arg Tyr Leu Asp Ala Met Ser Gly Leu Trp Cys Val 
    50                  55                  60                  


Asn Ile Gly Tyr Gly Arg Lys Glu Leu Ala Glu Ala Ala Tyr Glu Gln 
65                  70                  75                  80  


Leu Lys Glu Leu Pro Tyr Tyr Pro Leu Thr Gln Ser His Ala Pro Ala 
                85                  90                  95      


Ile Gln Leu Ala Glu Lys Leu Asn Glu Trp Leu Gly Gly Asp Tyr Val 
            100                 105                 110         


Ile Phe Phe Ser Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys 
        115                 120                 125             


Ile Ala Arg Gln Tyr His Leu Gln Asn Gly Asp His Ser Arg Tyr Lys 
    130                 135                 140                 


Phe Ile Ser Arg Tyr Arg Ala Tyr His Gly Asn Thr Leu Gly Ala Leu 
145                 150                 155                 160 


Ser Ala Thr Gly Gln Ala Gln Arg Lys Tyr Lys Tyr Glu Pro Leu Ser 
                165                 170                 175     


Gln Gly Phe Leu His Ala Ala Pro Pro Asp Ile Tyr Arg Asn Pro Asp 
            180                 185                 190         


Asp Ala Asp Thr Leu Glu Ser Ala Asn Glu Ile Asp Arg Ile Met Thr 
        195                 200                 205             


Trp Glu Leu Ser Glu Thr Ile Ala Gly Val Ile Met Glu Pro Ile Ile 
    210                 215                 220                 


Thr Gly Gly Gly Ile Leu Met Pro Pro Asp Gly Tyr Met Lys Lys Val 
225                 230                 235                 240 


Glu Asp Ile Cys Arg Arg His Gly Ala Leu Leu Ile Cys Asp Glu Val 
                245                 250                 255     


Ile Cys Gly Phe Gly Arg Thr Gly Glu Pro Phe Gly Phe Met His Tyr 
            260                 265                 270         


Gly Val Lys Pro Asp Ile Ile Thr Met Ala Lys Gly Ile Thr Ser Ala 
        275                 280                 285             


Tyr Leu Pro Leu Ser Ala Thr Ala Val Lys Arg Asp Ile Phe Glu Ala 
    290                 295                 300                 


Tyr Gln Gly Glu Ala Pro Tyr Asp Arg Phe Arg His Val Asn Thr Phe 
305                 310                 315                 320 


Gly Gly Ser Pro Ala Ala Cys Ala Leu Ala Leu Lys Asn Leu Gln Ile 
                325                 330                 335     


Met Glu Asp Glu Gln Leu Ile Gln Arg Ser Arg Asp Leu Gly Ala Lys 
            340                 345                 350         


Leu Leu Gly Glu Leu Gln Ala Leu Arg Glu His Pro Ala Val Gly Asp 
        355                 360                 365             


Val Arg Gly Lys Gly Leu Leu Ile Gly Ile Glu Leu Val Lys Asp Lys 
    370                 375                 380                 


Leu Thr Lys Glu Pro Ala Asp Ala Ala Lys Val Asn Gln Val Val Ala 
385                 390                 395                 400 


Ala Cys Lys Glu Lys Gly Leu Ile Ile Gly Lys Asn Gly Asp Thr Val 
                405                 410                 415     


Ala Gly Tyr Asn Asn Val Ile His Val Ala Pro Pro Phe Cys Leu Thr 
            420                 425                 430         


Glu Glu Asp Leu Ser Phe Ile Val Lys Thr Val Lys Glu Ser Phe Gln 
        435                 440                 445             


Thr Ile 
    450 


<210>  66
<211>  1407
<212>  DNA
<213>  Pseudomonas aeruginosa aminotransferase gene (seq ID gi9951072)

<400>  66
atgaacgcaa gactgcacgc cacgtccccc ctcggcgacg ccgacctggt ccgtgccgac     60

caggcccact acatgcacgg ctaccacgtg ttcgacgacc accgcgtcaa cggctcgctg    120

aacatcgccg ccggcgacgg cgcctatatc tacgacaccg ccggcaaccg ctacctcgac    180

gcggtgggcg gcatgtggtg caccaacatc ggcctggggc gcgaggaaat ggctcgcacc    240

gtggccgagc agacccgcct gctggcctat tccaatccct tctgcgacat ggccaacccg    300

cgcgccatcg aactctgccg caagctcgcc gagctggccc ccggcgacct cgaccacgtg    360

ttcctcacca ccggcggttc caccgccgtg gacaccgcga tccgcctcat gcactactac    420

cagaactgcc gcggcaagcg cgccaagaag cacgtcatca cgcggatcaa cgcctaccac    480

ggctcgacct tcctcggcat gtcgctgggc ggcaagagcg ccgaccggcc ggccgagttc    540

gacttcctcg acgagcgcat ccaccacctc gcctgtccct attactaccg cgctccggaa    600

gggctgggcg aagccgagtt cctcgatggc ctggtggacg agttcgaacg caagatcctc    660

gaactgggcg ccgaccgggt gggggcgttc atctccgagc cggtgttcgg ctccggcggc    720

gtgatcgtcc cgcccgcggg ctaccacagg cggatgtggg agctgtgcca gcgctacgac    780

gtgctgtaca tctccgacga agtggtgacc tccttcggcc gcctcggcca cttcttcgcc    840

agccaggcgg tgttcggcgt acagccggac atcatcctca ccgccaaggg cctcacctcc    900

ggctaccagc cgctgggcgc gtgcatcttc tcccggcgca tctgggaggt gatcgccgag    960

ccggacaagg gccgctgctt cagccatggt ttcacctact ccggccaccc ggtggcctgc   1020

gcggcggcgc tgaagaacat cgagatcatc gagcgcgagg gcttgctcgc ccacgccgac   1080

gaggtcggcc gctacttcga ggagcgcctg caaagcctcc gcgacctgcc catcgtcggc   1140

gacgtgcgcg ggatgcgctt catggcctgt gtcgagttcg tcgccgacaa ggcgagcaag   1200

gcgctgtttc cggaaagcct gaacatcggc gagtgggtcc acctgcgggc gcagaagcgc   1260

ggcctgctgg ttcgtccgat cgtccacctg aacgtgatgt cgccgccgct gatcctcacc   1320

cgcgaacagg tcgataccgt ggtccgggtg ctgcgcgaga gcatcgagga aaccgtggag   1380

gatcttgtcc gcgccggtca ccggtaa                                       1407


<210>  67
<211>  468
<212>  PRT
<213>  amino acid sequence of Pseudomonas aeruginosa  aminotransferase (seq ID gi9951072)

<400>  67

Met Asn Ala Arg Leu His Ala Thr Ser Pro Leu Gly Asp Ala Asp Leu 
1               5                   10                  15      


Val Arg Ala Asp Gln Ala His Tyr Met His Gly Tyr His Val Phe Asp 
            20                  25                  30          


Asp His Arg Val Asn Gly Ser Leu Asn Ile Ala Ala Gly Asp Gly Ala 
        35                  40                  45              


Tyr Ile Tyr Asp Thr Ala Gly Asn Arg Tyr Leu Asp Ala Val Gly Gly 
    50                  55                  60                  


Met Trp Cys Thr Asn Ile Gly Leu Gly Arg Glu Glu Met Ala Arg Thr 
65                  70                  75                  80  


Val Ala Glu Gln Thr Arg Leu Leu Ala Tyr Ser Asn Pro Phe Cys Asp 
                85                  90                  95      


Met Ala Asn Pro Arg Ala Ile Glu Leu Cys Arg Lys Leu Ala Glu Leu 
            100                 105                 110         


Ala Pro Gly Asp Leu Asp His Val Phe Leu Thr Thr Gly Gly Ser Thr 
        115                 120                 125             


Ala Val Asp Thr Ala Ile Arg Leu Met His Tyr Tyr Gln Asn Cys Arg 
    130                 135                 140                 


Gly Lys Arg Ala Lys Lys His Val Ile Thr Arg Ile Asn Ala Tyr His 
145                 150                 155                 160 


Gly Ser Thr Phe Leu Gly Met Ser Leu Gly Gly Lys Ser Ala Asp Arg 
                165                 170                 175     


Pro Ala Glu Phe Asp Phe Leu Asp Glu Arg Ile His His Leu Ala Cys 
            180                 185                 190         


Pro Tyr Tyr Tyr Arg Ala Pro Glu Gly Leu Gly Glu Ala Glu Phe Leu 
        195                 200                 205             


Asp Gly Leu Val Asp Glu Phe Glu Arg Lys Ile Leu Glu Leu Gly Ala 
    210                 215                 220                 


Asp Arg Val Gly Ala Phe Ile Ser Glu Pro Val Phe Gly Ser Gly Gly 
225                 230                 235                 240 


Val Ile Val Pro Pro Ala Gly Tyr His Arg Arg Met Trp Glu Leu Cys 
                245                 250                 255     


Gln Arg Tyr Asp Val Leu Tyr Ile Ser Asp Glu Val Val Thr Ser Phe 
            260                 265                 270         


Gly Arg Leu Gly His Phe Phe Ala Ser Gln Ala Val Phe Gly Val Gln 
        275                 280                 285             


Pro Asp Ile Ile Leu Thr Ala Lys Gly Leu Thr Ser Gly Tyr Gln Pro 
    290                 295                 300                 


Leu Gly Ala Cys Ile Phe Ser Arg Arg Ile Trp Glu Val Ile Ala Glu 
305                 310                 315                 320 


Pro Asp Lys Gly Arg Cys Phe Ser His Gly Phe Thr Tyr Ser Gly His 
                325                 330                 335     


Pro Val Ala Cys Ala Ala Ala Leu Lys Asn Ile Glu Ile Ile Glu Arg 
            340                 345                 350         


Glu Gly Leu Leu Ala His Ala Asp Glu Val Gly Arg Tyr Phe Glu Glu 
        355                 360                 365             


Arg Leu Gln Ser Leu Arg Asp Leu Pro Ile Val Gly Asp Val Arg Gly 
    370                 375                 380                 


Met Arg Phe Met Ala Cys Val Glu Phe Val Ala Asp Lys Ala Ser Lys 
385                 390                 395                 400 


Ala Leu Phe Pro Glu Ser Leu Asn Ile Gly Glu Trp Val His Leu Arg 
                405                 410                 415     


Ala Gln Lys Arg Gly Leu Leu Val Arg Pro Ile Val His Leu Asn Val 
            420                 425                 430         


Met Ser Pro Pro Leu Ile Leu Thr Arg Glu Gln Val Asp Thr Val Val 
        435                 440                 445             


Arg Val Leu Arg Glu Ser Ile Glu Glu Thr Val Glu Asp Leu Val Arg 
    450                 455                 460                 


Ala Gly His Arg 
465             


<210>  68
<211>  1335
<212>  DNA
<213>  Pseudomonas aeruginosa aminotransferase gene (seq ID gi9951630)

<400>  68
atgacaatga atgacgagcc gcagtcgagc agcctcgaca acttctggat gcccttcacc     60

gccaaccgcc agttcaaggc gcggccgcgc ctgctggaaa gcgccgaagg catccactat    120

atcgcccagg gcgggcgccg catcctcgac ggcaccgccg gcctctggtg ctgcaatgcc    180

ggccacggcc ggcgcgagat cagcgaagcg gtggcccggc agatcgccac cctcgactac    240

gccccgccgt tccagatggg tcacccgctg ccgttcgaac tcgccgcgcg gctgacggaa    300

atcgccccgc cgagcctgaa caaagtattc ttcaccaact ccggctcgga atcggcggac    360

accgcgctga agatcgccct tgcctaccag cgcgccatcg gccagggcac ccgcacccgc    420

ctgatcggcc gcgaactggg ctaccacggg gtcggcttcg gcggcctgtc ggtaggcggt    480

atggtcaaca accgcaaggc cttctccgcc aacctgctgc cgggggtcga ccacctgccg    540

cacaccctgg acgtcgcccg caacgccttc accgtcggcc tgcccgagca tggcgtggaa    600

aaggccgagg agctggaacg cctggtgacc ctgcacggcg ccgagaatat cgccgcggtg    660

atcgtcgagc cgatgtccgg ctcggccggc gtggtgctgc cgcccaaggg ctaccttcag    720

cggctgcgcg agataacccg caagcatggc atcctgctga tcttcgacga agtgatcacc    780

ggtttcggcc gcgtcggcga agccttcgcc gcgcagcgct ggggcgtcgt cccggacctg    840

ctgacctgcg ccaaggggct gaccaacggc agcatcccga tgggcgccgt attcgtcgac    900

gagaagatcc atgctgcctt catgcaaggc ccgcagggcg ccatcgagtt cttccacggc    960

tatacctatt ccggccatcc ggtagcctgc gccgccgccc tggcgaccct ggacatctac   1020

cgtcgcgacg acctgttcca gcgggccgtc gaactggaag gctactggca ggacgcgctg   1080

ttcagcctgc gcgacctgcc caacgtggtc gacatccgcg ccgtaggcct ggtcggcggc   1140

gtgcaactgg cgccgcacgc ggacggcccc ggcaagcgcg gctacgacgt cttcgagcgc   1200

tgcttctggg agcacgacct gatggtccgg gtgaccggcg acatcatcgc catgtcgccg   1260

ccgctgatca tcgacaagcc ccacatcgac cagatcgtcg agcgcctggc ccaggccatc   1320

cgcgccagcg tctga                                                    1335


<210>  69
<211>  444
<212>  PRT
<213>  - amino acid sequence of Pseudomonas aeruginosa  aminotransferase (seq ID gi9951630)

<400>  69

Met Thr Met Asn Asp Glu Pro Gln Ser Ser Ser Leu Asp Asn Phe Trp 
1               5                   10                  15      


Met Pro Phe Thr Ala Asn Arg Gln Phe Lys Ala Arg Pro Arg Leu Leu 
            20                  25                  30          


Glu Ser Ala Glu Gly Ile His Tyr Ile Ala Gln Gly Gly Arg Arg Ile 
        35                  40                  45              


Leu Asp Gly Thr Ala Gly Leu Trp Cys Cys Asn Ala Gly His Gly Arg 
    50                  55                  60                  


Arg Glu Ile Ser Glu Ala Val Ala Arg Gln Ile Ala Thr Leu Asp Tyr 
65                  70                  75                  80  


Ala Pro Pro Phe Gln Met Gly His Pro Leu Pro Phe Glu Leu Ala Ala 
                85                  90                  95      


Arg Leu Thr Glu Ile Ala Pro Pro Ser Leu Asn Lys Val Phe Phe Thr 
            100                 105                 110         


Asn Ser Gly Ser Glu Ser Ala Asp Thr Ala Leu Lys Ile Ala Leu Ala 
        115                 120                 125             


Tyr Gln Arg Ala Ile Gly Gln Gly Thr Arg Thr Arg Leu Ile Gly Arg 
    130                 135                 140                 


Glu Leu Gly Tyr His Gly Val Gly Phe Gly Gly Leu Ser Val Gly Gly 
145                 150                 155                 160 


Met Val Asn Asn Arg Lys Ala Phe Ser Ala Asn Leu Leu Pro Gly Val 
                165                 170                 175     


Asp His Leu Pro His Thr Leu Asp Val Ala Arg Asn Ala Phe Thr Val 
            180                 185                 190         


Gly Leu Pro Glu His Gly Val Glu Lys Ala Glu Glu Leu Glu Arg Leu 
        195                 200                 205             


Val Thr Leu His Gly Ala Glu Asn Ile Ala Ala Val Ile Val Glu Pro 
    210                 215                 220                 


Met Ser Gly Ser Ala Gly Val Val Leu Pro Pro Lys Gly Tyr Leu Gln 
225                 230                 235                 240 


Arg Leu Arg Glu Ile Thr Arg Lys His Gly Ile Leu Leu Ile Phe Asp 
                245                 250                 255     


Glu Val Ile Thr Gly Phe Gly Arg Val Gly Glu Ala Phe Ala Ala Gln 
            260                 265                 270         


Arg Trp Gly Val Val Pro Asp Leu Leu Thr Cys Ala Lys Gly Leu Thr 
        275                 280                 285             


Asn Gly Ser Ile Pro Met Gly Ala Val Phe Val Asp Glu Lys Ile His 
    290                 295                 300                 


Ala Ala Phe Met Gln Gly Pro Gln Gly Ala Ile Glu Phe Phe His Gly 
305                 310                 315                 320 


Tyr Thr Tyr Ser Gly His Pro Val Ala Cys Ala Ala Ala Leu Ala Thr 
                325                 330                 335     


Leu Asp Ile Tyr Arg Arg Asp Asp Leu Phe Gln Arg Ala Val Glu Leu 
            340                 345                 350         


Glu Gly Tyr Trp Gln Asp Ala Leu Phe Ser Leu Arg Asp Leu Pro Asn 
        355                 360                 365             


Val Val Asp Ile Arg Ala Val Gly Leu Val Gly Gly Val Gln Leu Ala 
    370                 375                 380                 


Pro His Ala Asp Gly Pro Gly Lys Arg Gly Tyr Asp Val Phe Glu Arg 
385                 390                 395                 400 


Cys Phe Trp Glu His Asp Leu Met Val Arg Val Thr Gly Asp Ile Ile 
                405                 410                 415     


Ala Met Ser Pro Pro Leu Ile Ile Asp Lys Pro His Ile Asp Gln Ile 
            420                 425                 430         


Val Glu Arg Leu Ala Gln Ala Ile Arg Ala Ser Val 
        435                 440                 


<210>  70
<211>  71
<212>  DNA
<213>  Forward primer for amplification of Bacillus subtilis aminotransferase (gi16077991) 

<400>  70
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatggaga tgatggggat     60

ggaaaacatt c                                                          71


<210>  71
<211>  65
<212>  DNA
<213>  Reverse primer for amplification of Bacillus subtilis aminotransferase (gi16077991) 

<400>  71
ggggaccact ttgtacaaga aagctgggtt tatatcgttt gaaagctttc tttcaccgtt     60

ttcac                                                                 65


<210>  72
<211>  66
<212>  DNA
<213>  Forward primer for amplification of Pseudomonas aeruginosa aminotransferase (gi9951072)

<400>  72
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgaacg caagactgca     60

cgccac                                                                66


<210>  73
<211>  48
<212>  DNA
<213>  Reverse primer for amplification of Pseudomonas aeruginosa aminotransferase (gi9951072) 

<400>  73
ggggaccact ttgtacaaga aagctgggtt taccggtgac cggcgcgg                  48


<210>  74
<211>  69
<212>  DNA
<213>  Forward primer for amplification of Pseudomonas aeruginosa aminotransferase (gi9951630)

<400>  74
ggggacaagt ttgtacaaaa aagcaggcta ggaggaatta accatgacaa tgaatgacga     60

gccgcagtc                                                             69


<210>  75
<211>  49
<212>  DNA
<213>  Reverse primer for amplification of Pseudomonas aeruginosa aminotransferase (gi9951630) 

<400>  75
ggggaccact ttgtacaaga aagctgggtt cagacgctgg cgcggatgg                 49


<210>  76
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer

<400>  76
aaatttacta gtaagaattt ttgaggaggc aatataaatg aataaaccac agtcttg        57


<210>  77
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer

<400>  77
aaatttggat cctacaagaa agctgggttt ac                                   32


<210>  78
<211>  58
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer

<400>  78
aaatttacta gtaagaattt ttgaggaggc aatataaatg aacagccaaa tcaccaac       58


<210>  79
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer

<400>  79
aaatttggat ccactttgta caagaaagct gggttca                              37


<210>  80
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer

<400>  80
aaatttggat ccgttgagga ggcctcaaaa atgtccgaga tcactctggg caaatac        57


<210>  81
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer

<400>  81
aaatttggcg cgccattact gtttagcgtt agttg                                35


<210>  82
<211>  52
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer

<400>  82
aaatttggat ccgttgagga ggcctcaaaa atgtatactg ttggtgatta tc             52


<210>  83
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer

<400>  83
aaatttggcg cgccattact tgttctgctc cgcaaac                              37





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