                         SEQUENCE LISTING

<110> Ceres, Inc.
 
<120> NITROGEN USE EFFICIENCY

<130> 4980-1706WO1

<150> 61/029,843
<151> 2008-02-19


<160> 54


<210> 1
<211> 1773
<212> DNA
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> NRT 1.3 

<220>
<221> misc_feature
<223> The DNA sequence encodes the protein given in SEQ ID NO. 2 

<400> 1
atggttcatg tgtcatcatc tcatggagcc aaagatggct ctgaagaagc ctttgattac  60    

agaggaaacc caccagataa gtctaaaacc ggtggatggt taggcgccgg tttaatttta  120   

gggagcgagc tatcagagag aatatgcgtg atgggcatat caatgaatct agtgacgtac  180   

cttgttggag atttacacat ctcatcagct aaatcagcga ccatagtcac caacttcatg  240   

ggaactctta accttctagg gcttctcggt ggttttttgg ctgacgctaa actcggtcgc  300   

tacaagatgg ttgcaatctc agcttctgtc acagctctgg gagtgttgct tttgacggtg  360   

gctacaacta tctcaagcat gagaccacca atatgtgacg atttcaggag acttcatcat  420   

cagtgcatag aagcaaacgg acaccagttg gctcttctct atgttgctct ctataccata  480   

gctctaggcg gaggaggaat caaatccaac gtctctggtt ttgggtctga ccagttcgat  540   

actagtgatc ctaaagaaga gaaacagatg attttcttct tcaacagatt ctatttctcc  600   

atcagcgttg gctctctctt cgccgtgatt gctcttgttt acgttcagga caacgtcggg  660   

agaggctggg gttacgggat ctctgccgcg actatggtgg ttgcagccat tgttttactc  720   

tgcggaacga aacggtaccg tttcaagaaa cctaaaggaa gcccttttac aacaatatgg  780   

agggttggtt tcttggcttg gaagaaaaga aaggagagtt accctgcgca tccaagtctt  840   

ttgaacggtt atgacaacac cacggttcca cacacagaga tgcttaagtg tttagacaaa  900   

gccgcaattt ccaagaacga gagctctcct agctcgaagg acttcgaaga gaaggatccg  960   

tggatcgttt cgactgttac acaagtcgaa gaagtgaaac tcgtgatgaa attggtaccg  1020  

atttgggcaa cgaacattct tttctggacg atttactccc aaatgacgac tttcacggtc  1080  

gaacaagcga cgtttatgga ccgaaaactc ggatctttca ctgttcctgc aggctcttac  1140  

tctgctttcc tcatactcac aattctcctc ttcacttccc ttaacgagag agtctttgtt  1200  

cctttaacaa gaaggctcac aaaaaagcct caaggaatca caagcctaca gagaatcgga  1260  

gtagggctag tattctcaat ggctgcaatg gctgtagccg cggttataga gaacgctaga  1320  

cgcgaggcag cggttaacaa cgataagaaa ataagcgcgt tttggttggt tccacaatat  1380  

ttcttagtcg gtgcgggtga ggcctttgct tacgttggac agcttgagtt ctttataaga  1440  

gaagcaccag agaggatgaa atcgatgagc accggattgt ttctaagcac gatatcgatg  1500  

ggattcttcg tgagtagctt gcttgtttcg cttgttgata gggttacaga caaaagctgg  1560  

cttagaagta accttaacaa agcgagattg aactacttct actggttact tgttgtcttg  1620  

ggagcattga acttcttgat ttttattgtg tttgccatga aacatcagta taaagctgat  1680  

gtgattactg ttgttgtgac tgatgatgat tcagtggaga aggaagtgac gaagaaagag  1740  

agctctgaat ttgagcttaa ggacattcct tga                               1773  


<210> 2
<211> 590
<212> PRT
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> NRT 1.3 

<220>
<221> misc_feature
<222> (101)..(498)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Bit score of 1463.4 for HMM based on sequences of FIGURE 11 

<400> 2
Met Val His Val Ser Ser Ser His Gly Ala Lys Asp Gly Ser Glu Glu 
1               5                   10                  15      

Ala Phe Asp Tyr Arg Gly Asn Pro Pro Asp Lys Ser Lys Thr Gly Gly 
            20                  25                  30          

Trp Leu Gly Ala Gly Leu Ile Leu Gly Ser Glu Leu Ser Glu Arg Ile 
        35                  40                  45              

Cys Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly Asp 
    50                  55                  60                  

Leu His Ile Ser Ser Ala Lys Ser Ala Thr Ile Val Thr Asn Phe Met 
65                  70                  75                  80  

Gly Thr Leu Asn Leu Leu Gly Leu Leu Gly Gly Phe Leu Ala Asp Ala 
                85                  90                  95      

Lys Leu Gly Arg Tyr Lys Met Val Ala Ile Ser Ala Ser Val Thr Ala 
            100                 105                 110         

Leu Gly Val Leu Leu Leu Thr Val Ala Thr Thr Ile Ser Ser Met Arg 
        115                 120                 125             

Pro Pro Ile Cys Asp Asp Phe Arg Arg Leu His His Gln Cys Ile Glu 
    130                 135                 140                 

Ala Asn Gly His Gln Leu Ala Leu Leu Tyr Val Ala Leu Tyr Thr Ile 
145                 150                 155                 160 

Ala Leu Gly Gly Gly Gly Ile Lys Ser Asn Val Ser Gly Phe Gly Ser 
                165                 170                 175     

Asp Gln Phe Asp Thr Ser Asp Pro Lys Glu Glu Lys Gln Met Ile Phe 
            180                 185                 190         

Phe Phe Asn Arg Phe Tyr Phe Ser Ile Ser Val Gly Ser Leu Phe Ala 
        195                 200                 205             

Val Ile Ala Leu Val Tyr Val Gln Asp Asn Val Gly Arg Gly Trp Gly 
    210                 215                 220                 

Tyr Gly Ile Ser Ala Ala Thr Met Val Val Ala Ala Ile Val Leu Leu 
225                 230                 235                 240 

Cys Gly Thr Lys Arg Tyr Arg Phe Lys Lys Pro Lys Gly Ser Pro Phe 
                245                 250                 255     

Thr Thr Ile Trp Arg Val Gly Phe Leu Ala Trp Lys Lys Arg Lys Glu 
            260                 265                 270         

Ser Tyr Pro Ala His Pro Ser Leu Leu Asn Gly Tyr Asp Asn Thr Thr 
        275                 280                 285             

Val Pro His Thr Glu Met Leu Lys Cys Leu Asp Lys Ala Ala Ile Ser 
    290                 295                 300                 

Lys Asn Glu Ser Ser Pro Ser Ser Lys Asp Phe Glu Glu Lys Asp Pro 
305                 310                 315                 320 

Trp Ile Val Ser Thr Val Thr Gln Val Glu Glu Val Lys Leu Val Met 
                325                 330                 335     

Lys Leu Val Pro Ile Trp Ala Thr Asn Ile Leu Phe Trp Thr Ile Tyr 
            340                 345                 350         

Ser Gln Met Thr Thr Phe Thr Val Glu Gln Ala Thr Phe Met Asp Arg 
        355                 360                 365             

Lys Leu Gly Ser Phe Thr Val Pro Ala Gly Ser Tyr Ser Ala Phe Leu 
    370                 375                 380                 

Ile Leu Thr Ile Leu Leu Phe Thr Ser Leu Asn Glu Arg Val Phe Val 
385                 390                 395                 400 

Pro Leu Thr Arg Arg Leu Thr Lys Lys Pro Gln Gly Ile Thr Ser Leu 
                405                 410                 415     

Gln Arg Ile Gly Val Gly Leu Val Phe Ser Met Ala Ala Met Ala Val 
            420                 425                 430         

Ala Ala Val Ile Glu Asn Ala Arg Arg Glu Ala Ala Val Asn Asn Asp 
        435                 440                 445             

Lys Lys Ile Ser Ala Phe Trp Leu Val Pro Gln Tyr Phe Leu Val Gly 
    450                 455                 460                 

Ala Gly Glu Ala Phe Ala Tyr Val Gly Gln Leu Glu Phe Phe Ile Arg 
465                 470                 475                 480 

Glu Ala Pro Glu Arg Met Lys Ser Met Ser Thr Gly Leu Phe Leu Ser 
                485                 490                 495     

Thr Ile Ser Met Gly Phe Phe Val Ser Ser Leu Leu Val Ser Leu Val 
            500                 505                 510         

Asp Arg Val Thr Asp Lys Ser Trp Leu Arg Ser Asn Leu Asn Lys Ala 
        515                 520                 525             

Arg Leu Asn Tyr Phe Tyr Trp Leu Leu Val Val Leu Gly Ala Leu Asn 
    530                 535                 540                 

Phe Leu Ile Phe Ile Val Phe Ala Met Lys His Gln Tyr Lys Ala Asp 
545                 550                 555                 560 

Val Ile Thr Val Val Val Thr Asp Asp Asp Ser Val Glu Lys Glu Val 
                565                 570                 575     

Thr Lys Lys Glu Ser Ser Glu Phe Glu Leu Lys Asp Ile Pro 
            580                 585                 590 


<210> 3
<211> 1998
<212> DNA
<213> Populus balsamifera subsp. trichocarpa

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 1445187 

<220>
<221> misc_feature
<223> The DNA sequence encodes the protein given in SEQ ID NO. 4 

<400> 3
atgcatctta ccaagacaca aaatcagaga gtgaagccct accatcctca attatctttc  60    

aattcccata acgatagtac gatcaagaag aaacaagaaa caagattgga acgctgggat  120   

ggtcattttc cagagttggc gaggttgtgg ctgcaggaac ctaaaccact agatgaatac  180   

gtccaagctg ctcacatggt tttagcagca ggcctgggcg gggaagaggg cgcacataat  240   

gatcttgcag tagactttcg aggaattcct gtggacaagt ccagaactgg aggatggctt  300   

gctgcggggc tcattctggt aaccgaactt tcggagagaa tatgtgtcat gggcatatca  360   

atgaatttag tgacatacct cgtcggagta ttgcatattt catcggcaaa atctgcaaac  420   

atggtcacca acttcatggg tactctcaac cttcttggcc tctttggagg tttcttggca  480   

gatgctaagc tcggtcggta cttgacggtt gcgatcttcg caataataac tgctttggcc  540   

atgctgctga ttcctttcca tatgcatcag ggggtcagtc tactaacagc agccacaacc  600   

attcctagca tgagaccccc accatgcgat gactacagga aacagaatca tcagtgcatt  660   

gaggtgaaag gaaatcaact ggttctgcta tatgcagcgc tttatactgt agcccttggt  720   

ggtggtggaa ttaagtccaa tgtctcaggt tttggatctg atcagttcga tcaaacagat  780   

cccaaggagg agaagacaat gattttcttc ttcaatcggt tttatttttg cattagcatg  840   

gggtcattgt ttgcagttat agtgctggta tatgtacaag ataacgtcgg aagagggtgg  900   

gggtatggaa tttcagcagg gacaatggtg atcgcagtag cagtgttgat ctgtgggact  960   

ccacggtacc gtttcaagaa gcctcaaggt agcccattaa ctatcatatg gagggtatcg  1020  

ttattggcat ggaagaaaag gagacatcct catccttcac atcccagcct tctgaatggg  1080  

tatgatagtg ccacagttcc ctactcagag aaactcaagt gtcttgacaa ggctgcaatc  1140  

atggatgatc ccacctccat cggtgcacaa aaaaatgacc cttggatagt atcaactgtt  1200  

atggaagttg aggaggtgaa gatggtacta aagctcatcc caatttggtc tacatgtatc  1260  

ctcttttgga cagtttactc tcaaatgact acttttacca ttgagcaagc aaccttcatg  1320  

gatcgaaaag ttggctcttt ggttattcct tcaggttcat actccacctt tctcttcatc  1380  

accattctcc tctttacatc cctaaatgaa aggctcttcg ttcccctggc tcgaaagctc  1440  

acccacaatg cacagggact gacaagcctc caaaggattg gagctggact ctttctttca  1500  

atactagcca tggtggctgc tgctagcgtt gagaaagaaa gaagggaaac tgctattcaa  1560  

caatctacaa agataagcgc cttctggcta gtccctcaat acttcctagt gggtgccggg  1620  

gaggctttcg tctatgttgg acaacttgag tttttcatca gagaggcacc agcaaggatg  1680  

aaatcaatga gcactgggct tttcctcagc accatctcaa tgggtttctt tgttagcagt  1740  

ttgctggtgt ctttagttga caaagctacc aagaaaatct ggctgaggag caacttgaac  1800  

aaggggaggt tgaataactt ctattggtta ctagcagtgc taggagggtt gaacttcttc  1860  

gttttcctag catttgcgag gggacatcag tacaaagtcc agcactatat caagcccaaa  1920  

aactgtaacg agaaagggtt taaacctgca ggtgatttga ctgctttaga gatggtgaag  1980  

gaggagggaa tgatttaa                                                1998  


<210> 4
<211> 600
<212> PRT
<213> Populus balsamifera subsp. trichocarpa

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 1445187 

<220>
<221> misc_feature
<222> (102)..(507)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of NRT 1.3 
      at SEQ ID NO. 2 
      with e-value of 6.20E-220 
      and BLAST sequence identity of 76.0% 

<220>
<221> misc_feature
<223> Bit score of 1461.3 for HMM based on sequences of FIGURE 11 

<400> 4
Met Val Leu Ala Ala Gly Leu Gly Gly Glu Glu Gly Ala His Asn Asp 
1               5                   10                  15      

Leu Ala Val Asp Phe Arg Gly Ile Pro Val Asp Lys Ser Arg Thr Gly 
            20                  25                  30          

Gly Trp Leu Ala Ala Gly Leu Ile Leu Val Thr Glu Leu Ser Glu Arg 
        35                  40                  45              

Ile Cys Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly 
    50                  55                  60                  

Val Leu His Ile Ser Ser Ala Lys Ser Ala Asn Met Val Thr Asn Phe 
65                  70                  75                  80  

Met Gly Thr Leu Asn Leu Leu Gly Leu Phe Gly Gly Phe Leu Ala Asp 
                85                  90                  95      

Ala Lys Leu Gly Arg Tyr Leu Thr Val Ala Ile Phe Ala Ile Ile Thr 
            100                 105                 110         

Ala Leu Ala Met Leu Leu Ile Pro Phe His Met His Gln Gly Val Ser 
        115                 120                 125             

Leu Leu Thr Ala Ala Thr Thr Ile Pro Ser Met Arg Pro Pro Pro Cys 
    130                 135                 140                 

Asp Asp Tyr Arg Lys Gln Asn His Gln Cys Ile Glu Val Lys Gly Asn 
145                 150                 155                 160 

Gln Leu Val Leu Leu Tyr Ala Ala Leu Tyr Thr Val Ala Leu Gly Gly 
                165                 170                 175     

Gly Gly Ile Lys Ser Asn Val Ser Gly Phe Gly Ser Asp Gln Phe Asp 
            180                 185                 190         

Gln Thr Asp Pro Lys Glu Glu Lys Thr Met Ile Phe Phe Phe Asn Arg 
        195                 200                 205             

Phe Tyr Phe Cys Ile Ser Met Gly Ser Leu Phe Ala Val Ile Val Leu 
    210                 215                 220                 

Val Tyr Val Gln Asp Asn Val Gly Arg Gly Trp Gly Tyr Gly Ile Ser 
225                 230                 235                 240 

Ala Gly Thr Met Val Ile Ala Val Ala Val Leu Ile Cys Gly Thr Pro 
                245                 250                 255     

Arg Tyr Arg Phe Lys Lys Pro Gln Gly Ser Pro Leu Thr Ile Ile Trp 
            260                 265                 270         

Arg Val Ser Leu Leu Ala Trp Lys Lys Arg Arg His Pro His Pro Ser 
        275                 280                 285             

His Pro Ser Leu Leu Asn Gly Tyr Asp Ser Ala Thr Val Pro Tyr Ser 
    290                 295                 300                 

Glu Lys Leu Lys Cys Leu Asp Lys Ala Ala Ile Met Asp Asp Pro Thr 
305                 310                 315                 320 

Ser Ile Gly Ala Gln Lys Asn Asp Pro Trp Ile Val Ser Thr Val Met 
                325                 330                 335     

Glu Val Glu Glu Val Lys Met Val Leu Lys Leu Ile Pro Ile Trp Ser 
            340                 345                 350         

Thr Cys Ile Leu Phe Trp Thr Val Tyr Ser Gln Met Thr Thr Phe Thr 
        355                 360                 365             

Ile Glu Gln Ala Thr Phe Met Asp Arg Lys Val Gly Ser Leu Val Ile 
    370                 375                 380                 

Pro Ser Gly Ser Tyr Ser Thr Phe Leu Phe Ile Thr Ile Leu Leu Phe 
385                 390                 395                 400 

Thr Ser Leu Asn Glu Arg Leu Phe Val Pro Leu Ala Arg Lys Leu Thr 
                405                 410                 415     

His Asn Ala Gln Gly Leu Thr Ser Leu Gln Arg Ile Gly Ala Gly Leu 
            420                 425                 430         

Phe Leu Ser Ile Leu Ala Met Val Ala Ala Ala Ser Val Glu Lys Glu 
        435                 440                 445             

Arg Arg Glu Thr Ala Ile Gln Gln Ser Thr Lys Ile Ser Ala Phe Trp 
    450                 455                 460                 

Leu Val Pro Gln Tyr Phe Leu Val Gly Ala Gly Glu Ala Phe Val Tyr 
465                 470                 475                 480 

Val Gly Gln Leu Glu Phe Phe Ile Arg Glu Ala Pro Ala Arg Met Lys 
                485                 490                 495     

Ser Met Ser Thr Gly Leu Phe Leu Ser Thr Ile Ser Met Gly Phe Phe 
            500                 505                 510         

Val Ser Ser Leu Leu Val Ser Leu Val Asp Lys Ala Thr Lys Lys Ile 
        515                 520                 525             

Trp Leu Arg Ser Asn Leu Asn Lys Gly Arg Leu Asn Asn Phe Tyr Trp 
    530                 535                 540                 

Leu Leu Ala Val Leu Gly Gly Leu Asn Phe Phe Val Phe Leu Ala Phe 
545                 550                 555                 560 

Ala Arg Gly His Gln Tyr Lys Val Gln His Tyr Ile Lys Pro Lys Asn 
                565                 570                 575     

Cys Asn Glu Lys Gly Phe Lys Pro Ala Gly Asp Leu Thr Ala Leu Glu 
            580                 585                 590         

Met Val Lys Glu Glu Gly Met Ile 
        595                 600 


<210> 5
<211> 1965
<212> DNA
<213> Populus balsamifera subsp. trichocarpa

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 1498169 

<220>
<221> misc_feature
<223> The DNA sequence encodes the protein given in SEQ ID NO. 6 

<400> 5
atgcatctta ccaagacaca aaatcagaga gtgaagccct accatcctca attatctttc  60    

aattcccata acgatagtac gatcaagaag aaacaagaaa caagattgga acgctgggat  120   

ggtcattttc cagagttggc gaggttgtgg ctgcaggaac ctaaaccact agatgaatac  180   

gtccaagctg ctcacatggt tttagcagca ggcctgggcg gggaagaggg cgcacataat  240   

gatcttgcag tagactttcg aggaattcct gtggacaagt ccagaactgg aggatggctt  300   

gctgcggggc tcattctggt aaccgaactt tcggagagaa tatgtgtcat gggcatatca  360   

atgaatttag tgacatacct cgtcggagta ttgcatattt catcggcaaa atctgcaaac  420   

atggtcacca acttcatggg tactctcaac cttcttggcc tctttggagg tttcttggca  480   

gatgctaagc tcggtcggta cttgacggtt gcgatcttcg caataataac tgctttgggg  540   

gtcagtctac taacagcagc cacaaccatt cctagcatga gacccccacc atgcgatgac  600   

tacaggaaac agaatcatca gtgcattgag gtgaaaggaa atcaactggt tctgctatat  660   

gcagcgcttt atactgtagc ccttggtggt ggtggaatta agtccaatgt ctcaggtttt  720   

ggatctgatc agttcgatca aacagatccc aaggaggaga agacaatgat tttcttcttc  780   

aatcggtttt atttttgcat tagcatgggg tcattgtttg cagttatagt gctggtatat  840   

gtacaagata acgtcggaag agggtggggg tatggaattt cagcagggac aatggtgatc  900   

gcagtagcag tgttgatctg tgggactcca cggtaccgtt tcaagaagcc tcaaggtagc  960   

ccattaacta tcatatggag ggtatcgtta ttggcatgga agaaaaggag acatcctcat  1020  

ccttcacatc ccagccttct gaatgggtat gatagtgcca cagttcccta ctcagagaaa  1080  

ctcaagtgtc ttgacaaggc tgcaatcatg gatgatccca cctccatcgg tgcacaaaaa  1140  

aatgaccctt ggatagtatc aactgttatg gaagttgagg aggtgaagat ggtactaaag  1200  

ctcatcccaa tttggtctac atgtatcctc ttttggacag tttactctca aatgactact  1260  

tttaccattg agcaagcaac cttcatggat cgaaaagttg gctctttggt tattccttca  1320  

ggttcatact ccacctttct cttcatcacc attctcctct ttacatccct aaatgaaagg  1380  

ctcttcgttc ccctggctcg aaagctcacc cacaatgcac agggactgac aagcctccaa  1440  

aggattggag ctggactctt tctttcaata ctagccatgg tggctgctgc tagcgttgag  1500  

aaagaaagaa gggaaactgc tattcaacaa tctacaaaga taagcgcctt ctggctagtc  1560  

cctcaatact tcctagtggg tgccggggag gctttcgtct atgttggaca acttgagttt  1620  

ttcatcagag aggcaccagc aaggatgaaa tcaatgagca ctgggctttt cctcagcacc  1680  

atctcaatgg gtttctttgt tagcagtttg ctggtgtctt tagttgacaa agctaccaag  1740  

aaaatctggc tgaggagcaa cttgaacaag gggaggttga ataacttcta ttggttacta  1800  

gcagtgctag gagggttgaa cttcttcgtt ttcctagcat ttgcgagggg acatcagtac  1860  

aaagtccagc actatatcaa gcccaaaaac tgtaacgaga aagggtttaa acctgcaggt  1920  

gatttgactg ctttagagat ggtgaaggag gagggaatga tttaa                  1965  


<210> 6
<211> 589
<212> PRT
<213> Populus balsamifera subsp. trichocarpa

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 1498169 

<220>
<221> misc_feature
<222> (102)..(496)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of NRT 1.3 
      at SEQ ID NO. 2 
      with e-value of 5.19E-223 
      and BLAST sequence identity of 76.0% 

<220>
<221> misc_feature
<223> Bit score of 1482.3 for HMM based on sequences of FIGURE 11 

<400> 6
Met Val Leu Ala Ala Gly Leu Gly Gly Glu Glu Gly Ala His Asn Asp 
1               5                   10                  15      

Leu Ala Val Asp Phe Arg Gly Ile Pro Val Asp Lys Ser Arg Thr Gly 
            20                  25                  30          

Gly Trp Leu Ala Ala Gly Leu Ile Leu Val Thr Glu Leu Ser Glu Arg 
        35                  40                  45              

Ile Cys Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly 
    50                  55                  60                  

Val Leu His Ile Ser Ser Ala Lys Ser Ala Asn Met Val Thr Asn Phe 
65                  70                  75                  80  

Met Gly Thr Leu Asn Leu Leu Gly Leu Phe Gly Gly Phe Leu Ala Asp 
                85                  90                  95      

Ala Lys Leu Gly Arg Tyr Leu Thr Val Ala Ile Phe Ala Ile Ile Thr 
            100                 105                 110         

Ala Leu Gly Val Ser Leu Leu Thr Ala Ala Thr Thr Ile Pro Ser Met 
        115                 120                 125             

Arg Pro Pro Pro Cys Asp Asp Tyr Arg Lys Gln Asn His Gln Cys Ile 
    130                 135                 140                 

Glu Val Lys Gly Asn Gln Leu Val Leu Leu Tyr Ala Ala Leu Tyr Thr 
145                 150                 155                 160 

Val Ala Leu Gly Gly Gly Gly Ile Lys Ser Asn Val Ser Gly Phe Gly 
                165                 170                 175     

Ser Asp Gln Phe Asp Gln Thr Asp Pro Lys Glu Glu Lys Thr Met Ile 
            180                 185                 190         

Phe Phe Phe Asn Arg Phe Tyr Phe Cys Ile Ser Met Gly Ser Leu Phe 
        195                 200                 205             

Ala Val Ile Val Leu Val Tyr Val Gln Asp Asn Val Gly Arg Gly Trp 
    210                 215                 220                 

Gly Tyr Gly Ile Ser Ala Gly Thr Met Val Ile Ala Val Ala Val Leu 
225                 230                 235                 240 

Ile Cys Gly Thr Pro Arg Tyr Arg Phe Lys Lys Pro Gln Gly Ser Pro 
                245                 250                 255     

Leu Thr Ile Ile Trp Arg Val Ser Leu Leu Ala Trp Lys Lys Arg Arg 
            260                 265                 270         

His Pro His Pro Ser His Pro Ser Leu Leu Asn Gly Tyr Asp Ser Ala 
        275                 280                 285             

Thr Val Pro Tyr Ser Glu Lys Leu Lys Cys Leu Asp Lys Ala Ala Ile 
    290                 295                 300                 

Met Asp Asp Pro Thr Ser Ile Gly Ala Gln Lys Asn Asp Pro Trp Ile 
305                 310                 315                 320 

Val Ser Thr Val Met Glu Val Glu Glu Val Lys Met Val Leu Lys Leu 
                325                 330                 335     

Ile Pro Ile Trp Ser Thr Cys Ile Leu Phe Trp Thr Val Tyr Ser Gln 
            340                 345                 350         

Met Thr Thr Phe Thr Ile Glu Gln Ala Thr Phe Met Asp Arg Lys Val 
        355                 360                 365             

Gly Ser Leu Val Ile Pro Ser Gly Ser Tyr Ser Thr Phe Leu Phe Ile 
    370                 375                 380                 

Thr Ile Leu Leu Phe Thr Ser Leu Asn Glu Arg Leu Phe Val Pro Leu 
385                 390                 395                 400 

Ala Arg Lys Leu Thr His Asn Ala Gln Gly Leu Thr Ser Leu Gln Arg 
                405                 410                 415     

Ile Gly Ala Gly Leu Phe Leu Ser Ile Leu Ala Met Val Ala Ala Ala 
            420                 425                 430         

Ser Val Glu Lys Glu Arg Arg Glu Thr Ala Ile Gln Gln Ser Thr Lys 
        435                 440                 445             

Ile Ser Ala Phe Trp Leu Val Pro Gln Tyr Phe Leu Val Gly Ala Gly 
    450                 455                 460                 

Glu Ala Phe Val Tyr Val Gly Gln Leu Glu Phe Phe Ile Arg Glu Ala 
465                 470                 475                 480 

Pro Ala Arg Met Lys Ser Met Ser Thr Gly Leu Phe Leu Ser Thr Ile 
                485                 490                 495     

Ser Met Gly Phe Phe Val Ser Ser Leu Leu Val Ser Leu Val Asp Lys 
            500                 505                 510         

Ala Thr Lys Lys Ile Trp Leu Arg Ser Asn Leu Asn Lys Gly Arg Leu 
        515                 520                 525             

Asn Asn Phe Tyr Trp Leu Leu Ala Val Leu Gly Gly Leu Asn Phe Phe 
    530                 535                 540                 

Val Phe Leu Ala Phe Ala Arg Gly His Gln Tyr Lys Val Gln His Tyr 
545                 550                 555                 560 

Ile Lys Pro Lys Asn Cys Asn Glu Lys Gly Phe Lys Pro Ala Gly Asp 
                565                 570                 575     

Leu Thr Ala Leu Glu Met Val Lys Glu Glu Gly Met Ile 
            580                 585                 


<210> 7
<211> 2017
<212> DNA
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> Ceres CLONE ID no. 111089 

<220>
<221> misc_feature
<223> The DNA sequence encodes the protein given in SEQ ID NO. 8 

<400> 7
atcttaaact tctccttata actcacgctt ctctgctttc ttctctcaaa gtctaactgc  60    

tcaaataaga aaatggttca tgtgtcatca tctcatggag ccaaagatgg ctctgaagaa  120   

tcctttgatt acagaggaaa cccaccagat aagtctaaaa ccggtggatg gttaggcgcc  180   

ggtttaattt tagggagcga gctatcagag agaatatgcg tgatgggcat atcaatgaat  240   

ctagtgacgt accttgttgg agatttacac atctcatcag ctaaatcagc gaccatagtc  300   

accaacttca tgggaactct taaccttcta gggcttctcg gtggtttttt ggctgacgct  360   

aaactcggtc gctacaagat ggttgcaatc tcagcttctg tcacagctct gggagtgttg  420   

cttttgacgg tggctacaac tatctcaagc atgagaccac caatatgtga cgatttcagg  480   

agacttcatc atcagtgcat agaagcaaac ggacaccagt tggctcttct ctatgttgct  540   

ctctatacca tagctctagg cggaggagga atcaaatcca acgtctctgg ttttgggtct  600   

gaccagttcg atactagtga tcctaaagaa gagaaacaga tgattttctt cttcaacaga  660   

ttctatttct ccatcagcgt tggctctctc ttcgccgtga ttgctcttgt ttacgttcag  720   

gacaacgtcg ggagaggctg gggttacggg atctctgccg cgactatggt ggttgcagcc  780   

attgttttac tctgcggaac gaaacggtac cgtttcaaga aacctaaagg aagccctttt  840   

acaacaatat ggagggttgg tttcttggct tggaagaaaa gaaaggagag ttaccctgcg  900   

catccaagtc ttttgaacgg ttatgacaac accacggttc cacacacaga gatgcttaag  960   

tgtttaaaca aagccgcaat ttccaagaac gagagctctc ctagctcgaa ggacttcgaa  1020  

gagaaggatc cgtggatcgt ttcgactgtt acacaagtcg aagaagtgaa actcgtgatg  1080  

aaattggtac cgatttgggc aacgaacatt cttttctgga cgatttactc ccaaatgacg  1140  

actttcacgg tcgaacaagc gacgtttatg gaccgaaaac tcggatcttt cactgttcct  1200  

gcaggctctt actctgcttt cctcatactc acaattctcc tcttcacttc ccttaacgag  1260  

agagtctttg ttcctttaac aagaaggctc acaaaaaagc ctcaaggaat cacaagccta  1320  

cagagaatcg gagtagggct agtattctca atggctgcaa tggctgtagc cgcggttata  1380  

gagaacgcta gacgcgaggc agcggttaac aacgataaga aaataagcgc gttttggttg  1440  

gttccacaat atttcttagt cggtgcgggt gaggcctttg cttacgttgg acagcttgag  1500  

ttctttataa gagaagcacc agagaggatg aaatcgatga gcaccggatt gtttctaagc  1560  

acgatatcga tgggattctt cgtgagtagc ttgcttgttt cgcttgttga tagggttaca  1620  

gacaaaagct ggcttagaag taaccttaac aaagcgagat tgaactactt ctactggtta  1680  

cttgttgtct tgggagcatt gaacttcttg atttttattg tgtttgccat gaaacatcag  1740  

tataaagctg atgtgattac tgttgttgtg actgatgatg attcagtgga gaaggaagtg  1800  

acgaagaaag agagctctga atttgagctt aaggacattc cttgaaacca gatttagaag  1860  

aagaaaaaaa acctctggtt ttgttatttg ctttgaagat ttcattttgt ctttgtattt  1920  

ttagtatatt gtgtgtatga aatagaaaga ttactatatg gtttggattg tatttttttt  1980  

gtaagatttt taaaaattta gttactatag ttttttc                           2017  


<210> 8
<211> 590
<212> PRT
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> Ceres CLONE ID no. 111089 

<220>
<221> misc_feature
<222> (101)..(498)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of  NRT 1.3 
      at SEQ ID NO. 2 
      with e-value of 1.70E-311 
      and BLAST sequence identity of 99.6% 

<220>
<221> misc_feature
<223> Bit score of 1458.2 for HMM based on sequences of FIGURE 11 

<400> 8
Met Val His Val Ser Ser Ser His Gly Ala Lys Asp Gly Ser Glu Glu 
1               5                   10                  15      

Ser Phe Asp Tyr Arg Gly Asn Pro Pro Asp Lys Ser Lys Thr Gly Gly 
            20                  25                  30          

Trp Leu Gly Ala Gly Leu Ile Leu Gly Ser Glu Leu Ser Glu Arg Ile 
        35                  40                  45              

Cys Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly Asp 
    50                  55                  60                  

Leu His Ile Ser Ser Ala Lys Ser Ala Thr Ile Val Thr Asn Phe Met 
65                  70                  75                  80  

Gly Thr Leu Asn Leu Leu Gly Leu Leu Gly Gly Phe Leu Ala Asp Ala 
                85                  90                  95      

Lys Leu Gly Arg Tyr Lys Met Val Ala Ile Ser Ala Ser Val Thr Ala 
            100                 105                 110         

Leu Gly Val Leu Leu Leu Thr Val Ala Thr Thr Ile Ser Ser Met Arg 
        115                 120                 125             

Pro Pro Ile Cys Asp Asp Phe Arg Arg Leu His His Gln Cys Ile Glu 
    130                 135                 140                 

Ala Asn Gly His Gln Leu Ala Leu Leu Tyr Val Ala Leu Tyr Thr Ile 
145                 150                 155                 160 

Ala Leu Gly Gly Gly Gly Ile Lys Ser Asn Val Ser Gly Phe Gly Ser 
                165                 170                 175     

Asp Gln Phe Asp Thr Ser Asp Pro Lys Glu Glu Lys Gln Met Ile Phe 
            180                 185                 190         

Phe Phe Asn Arg Phe Tyr Phe Ser Ile Ser Val Gly Ser Leu Phe Ala 
        195                 200                 205             

Val Ile Ala Leu Val Tyr Val Gln Asp Asn Val Gly Arg Gly Trp Gly 
    210                 215                 220                 

Tyr Gly Ile Ser Ala Ala Thr Met Val Val Ala Ala Ile Val Leu Leu 
225                 230                 235                 240 

Cys Gly Thr Lys Arg Tyr Arg Phe Lys Lys Pro Lys Gly Ser Pro Phe 
                245                 250                 255     

Thr Thr Ile Trp Arg Val Gly Phe Leu Ala Trp Lys Lys Arg Lys Glu 
            260                 265                 270         

Ser Tyr Pro Ala His Pro Ser Leu Leu Asn Gly Tyr Asp Asn Thr Thr 
        275                 280                 285             

Val Pro His Thr Glu Met Leu Lys Cys Leu Asn Lys Ala Ala Ile Ser 
    290                 295                 300                 

Lys Asn Glu Ser Ser Pro Ser Ser Lys Asp Phe Glu Glu Lys Asp Pro 
305                 310                 315                 320 

Trp Ile Val Ser Thr Val Thr Gln Val Glu Glu Val Lys Leu Val Met 
                325                 330                 335     

Lys Leu Val Pro Ile Trp Ala Thr Asn Ile Leu Phe Trp Thr Ile Tyr 
            340                 345                 350         

Ser Gln Met Thr Thr Phe Thr Val Glu Gln Ala Thr Phe Met Asp Arg 
        355                 360                 365             

Lys Leu Gly Ser Phe Thr Val Pro Ala Gly Ser Tyr Ser Ala Phe Leu 
    370                 375                 380                 

Ile Leu Thr Ile Leu Leu Phe Thr Ser Leu Asn Glu Arg Val Phe Val 
385                 390                 395                 400 

Pro Leu Thr Arg Arg Leu Thr Lys Lys Pro Gln Gly Ile Thr Ser Leu 
                405                 410                 415     

Gln Arg Ile Gly Val Gly Leu Val Phe Ser Met Ala Ala Met Ala Val 
            420                 425                 430         

Ala Ala Val Ile Glu Asn Ala Arg Arg Glu Ala Ala Val Asn Asn Asp 
        435                 440                 445             

Lys Lys Ile Ser Ala Phe Trp Leu Val Pro Gln Tyr Phe Leu Val Gly 
    450                 455                 460                 

Ala Gly Glu Ala Phe Ala Tyr Val Gly Gln Leu Glu Phe Phe Ile Arg 
465                 470                 475                 480 

Glu Ala Pro Glu Arg Met Lys Ser Met Ser Thr Gly Leu Phe Leu Ser 
                485                 490                 495     

Thr Ile Ser Met Gly Phe Phe Val Ser Ser Leu Leu Val Ser Leu Val 
            500                 505                 510         

Asp Arg Val Thr Asp Lys Ser Trp Leu Arg Ser Asn Leu Asn Lys Ala 
        515                 520                 525             

Arg Leu Asn Tyr Phe Tyr Trp Leu Leu Val Val Leu Gly Ala Leu Asn 
    530                 535                 540                 

Phe Leu Ile Phe Ile Val Phe Ala Met Lys His Gln Tyr Lys Ala Asp 
545                 550                 555                 560 

Val Ile Thr Val Val Val Thr Asp Asp Asp Ser Val Glu Lys Glu Val 
                565                 570                 575     

Thr Lys Lys Glu Ser Ser Glu Phe Glu Leu Lys Asp Ile Pro 
            580                 585                 590 


<210> 9
<211> 2431
<212> DNA
<213> Zea mays

<220>
<221> misc_feature
<223> Ceres CLONE ID no. 1583036 

<220>
<221> misc_feature
<223> The DNA sequence encodes the protein given in SEQ ID NO. 10 

<400> 9
gccgagctca gtagcgagag cttgtaacct aagcgggggc attaaggtgg tgtggatgtt     60

tcctgacgga ttctgggcct gggcctgtat gtgccactgg ccggcctgcc cgttccaagt    120

tggttgtggc tggtggggct cgggcgaagg cctgggcctc tttagacctg aagcggcagg    180

catggcgtga ggctggcttc acagagcagc gatcactgcc cgcttccaac ggtgggagga    240

taacaggccg ctgcattgca agcccaactc ggtccagagc ctcattaaac agaccaccat    300

ctttgacaaa ctcacagcac aaacgttcct ctcctcttcc atcgtcgtcc tcgctcccgg    360

ccccgcctgt ctagtctagt cccctcttct cgcgtgcaac ccctgtactg tacgtgctag    420

gcacacagct agctgcagct acagcagggc agcaaatggt ttctggtgcg ggtcatggtg    480

ggtacggcgg cggcgacgac gggcaggccg tcgacttccg gggcaacccg gcggacaagt    540

cgaggaccgg aggctggctg ggcgccgggc tgatcctggg cacggagctg gcggagcgcg    600

tgtgcgtgat gggcatctcc atgaacctgg tgacgtacct cgtcggcgag ctgcacctct    660

ccaactccaa gtccgccacc gtggtgacca acttcatggg cacgctcaac ctgctcgccc    720

tcgtcggcgg cttcctcgcc gacgccaagc tcggccgcta cctcaccatc gccatctccg    780

ccacaatcgc cgccacgggc gtgagcttgc tgacggtgga cacgacggtg ccgagcatgc    840

gtccgccggc gtgcctggac gcccgcgggc cgcgcgcgca cgagtgcgtg ccggcgcgcg    900

gcgggcagct ggcgctgctg tacgcggcgc tgtacacggt ggcggcgggg gccggcgggc    960

tcaaggctaa cgtgtccggg ttcgggtcgg accagttcga cgggcgcgac ccgcgggagg   1020

agcgcgccat ggtgttcttc ttcaaccgct tctacttctg cgtcagcctg gggtcgctgt   1080

tcgcggtcac cgtgctggtg tacgtgcagg acaacgtggg gcggggctgg ggctacggcg   1140

tctccgccgt cgccatggcg ctcgccgtcg ccgtgctcgt ggccggcacg ccccggtaca   1200

ggtaccgccg cccgcagggc agcccgctca cggcggtcgg ccgggtgctc gccgcggcgt   1260

ggaggaagcg ccggctgccg ctgcccgccg acgccgccga gctccacggg ttcgccgcgg   1320

ccaaggtcgc acacactgac aggctcaggt ggcttgacaa ggcggcgatc gtggaggccg   1380

agccggcggg gaagcagcgg gcgagcgcgg cagcggcgtc gacggtgacg gaggtggagg   1440

aggtgaagat ggtggcgaag ctgctgccca tctggtccac gtgcatcctc ttctggacgg   1500

tctactccca gatgaccacc ttctcggtgg agcaggccac gcgcatggac cgccacctcc   1560

gcccgggctc cggcggcttc gccgtcccgg cgggctcctt ctccgtcttc ctattcctct   1620

ccatcctgct cttcacctcg ctcaacgagc gcctcctcgt gccgctggcc gcccgcctca   1680

cgggccgccc gcaggggctc acctcgctgc agcgcgtcgg ggccgggctc gcgctctccg   1740

tcgccgccat ggccgtctcc gcgctcgtcg agaggaagcg gcgcgacgcg gccaacgggc   1800

cgggccacgt cgccgtcagc gccttctggc tcgtcccgca gtacttcctc gtcggcgccg   1860

gcgaggcctt cgcctacgtg ggccagctgg agttcttcat ccgcgaggcc cccgagcgga   1920

tgaagtccat gagcaccggc ctcttcctcg tcacgctctc catgggcttc ttcctcagca   1980

gcttcctcgt cttcgccgtc gacgccgcca ccgcgggcac gtggatccgg aacaacctcg   2040

accgcggcag gctcgacctc ttctactgga tgctggccct gctcggcgtc gccaacttcg   2100

ccgtcttcgt cgtcttcgcc aggcggcacc agtacaaggc caccagcttg ccgtcgtcgg   2160

tggcgcccga cggcgccggg cacaaggaga tggacgactt cgtcgcagtc acggaggccg   2220

tggaaggagt ggacgtgtag ggcgcgcatg tcagactcac acttcatcgg tgggtgtggg   2280

tggcctctgc tgtttaattt tgcctttggg tagtgtgtat gtgtatggcg aagttttctt   2340

tctcttgcca gtttccagtt tgctgctgct tcggcctgct ctcctgtaaa taacatagga   2400

gacatgctaa ttgaggccaa gtctttatcc g                                  2431

<210> 10
<211> 594
<212> PRT
<213> Zea mays

<220>
<221> misc_feature
<223> Ceres CLONE ID no. 1583036 

<220>
<221> misc_feature
<222> (102)..(501)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of NRT 1.3 
      at SEQ ID NO. 2 
      with e-value of 9.19E-194 
      and BLAST sequence identity of 65.2% 

<220>
<221> misc_feature
<223> Bit score of 1467 for HMM based on sequences of FIGURE 11 

<400> 10
Met Val Ser Gly Ala Gly His Gly Gly Tyr Gly Gly Gly Asp Asp Gly 
1               5                   10                  15      

Gln Ala Val Asp Phe Arg Gly Asn Pro Ala Asp Lys Ser Arg Thr Gly 
            20                  25                  30          

Gly Trp Leu Gly Ala Gly Leu Ile Leu Gly Thr Glu Leu Ala Glu Arg 
        35                  40                  45              

Val Cys Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly 
    50                  55                  60                  

Glu Leu His Leu Ser Asn Ser Lys Ser Ala Thr Val Val Thr Asn Phe 
65                  70                  75                  80  

Met Gly Thr Leu Asn Leu Leu Ala Leu Val Gly Gly Phe Leu Ala Asp 
                85                  90                  95      

Ala Lys Leu Gly Arg Tyr Leu Thr Ile Ala Ile Ser Ala Thr Ile Ala 
            100                 105                 110         

Ala Thr Gly Val Ser Leu Leu Thr Val Asp Thr Thr Val Pro Ser Met 
        115                 120                 125             

Arg Pro Pro Ala Cys Leu Asp Ala Arg Gly Pro Arg Ala His Glu Cys 
    130                 135                 140                 

Val Pro Ala Arg Gly Gly Gln Leu Ala Leu Leu Tyr Ala Ala Leu Tyr 
145                 150                 155                 160 

Thr Val Ala Ala Gly Ala Gly Gly Leu Lys Ala Asn Val Ser Gly Phe 
                165                 170                 175     

Gly Ser Asp Gln Phe Asp Gly Arg Asp Pro Arg Glu Glu Arg Ala Met 
            180                 185                 190         

Val Phe Phe Phe Asn Arg Phe Tyr Phe Cys Val Ser Leu Gly Ser Leu 
        195                 200                 205             

Phe Ala Val Thr Val Leu Val Tyr Val Gln Asp Asn Val Gly Arg Gly 
    210                 215                 220                 

Trp Gly Tyr Gly Val Ser Ala Val Ala Met Ala Leu Ala Val Ala Val 
225                 230                 235                 240 

Leu Val Ala Gly Thr Pro Arg Tyr Arg Tyr Arg Arg Pro Gln Gly Ser 
                245                 250                 255     

Pro Leu Thr Ala Val Gly Arg Val Leu Ala Ala Ala Trp Arg Lys Arg 
            260                 265                 270         

Arg Leu Pro Leu Pro Ala Asp Ala Ala Glu Leu His Gly Phe Ala Ala 
        275                 280                 285             

Ala Lys Val Ala His Thr Asp Arg Leu Arg Trp Leu Asp Lys Ala Ala 
    290                 295                 300                 

Ile Val Glu Ala Glu Pro Ala Gly Lys Gln Arg Ala Ser Ala Ala Ala 
305                 310                 315                 320 

Ala Ser Thr Val Thr Glu Val Glu Glu Val Lys Met Val Ala Lys Leu 
                325                 330                 335     

Leu Pro Ile Trp Ser Thr Cys Ile Leu Phe Trp Thr Val Tyr Ser Gln 
            340                 345                 350         

Met Thr Thr Phe Ser Val Glu Gln Ala Thr Arg Met Asp Arg His Leu 
        355                 360                 365             

Arg Pro Gly Ser Gly Gly Phe Ala Val Pro Ala Gly Ser Phe Ser Val 
    370                 375                 380                 

Phe Leu Phe Leu Ser Ile Leu Leu Phe Thr Ser Leu Asn Glu Arg Leu 
385                 390                 395                 400 

Leu Val Pro Leu Ala Ala Arg Leu Thr Gly Arg Pro Gln Gly Leu Thr 
                405                 410                 415     

Ser Leu Gln Arg Val Gly Ala Gly Leu Ala Leu Ser Val Ala Ala Met 
            420                 425                 430         

Ala Val Ser Ala Leu Val Glu Arg Lys Arg Arg Asp Ala Ala Asn Gly 
        435                 440                 445             

Pro Gly His Val Ala Val Ser Ala Phe Trp Leu Val Pro Gln Tyr Phe 
    450                 455                 460                 

Leu Val Gly Ala Gly Glu Ala Phe Ala Tyr Val Gly Gln Leu Glu Phe 
465                 470                 475                 480 

Phe Ile Arg Glu Ala Pro Glu Arg Met Lys Ser Met Ser Thr Gly Leu 
                485                 490                 495     

Phe Leu Val Thr Leu Ser Met Gly Phe Phe Leu Ser Ser Phe Leu Val 
            500                 505                 510         

Phe Ala Val Asp Ala Ala Thr Ala Gly Thr Trp Ile Arg Asn Asn Leu 
        515                 520                 525             

Asp Arg Gly Arg Leu Asp Leu Phe Tyr Trp Met Leu Ala Leu Leu Gly 
    530                 535                 540                 

Val Ala Asn Phe Ala Val Phe Val Val Phe Ala Arg Arg His Gln Tyr 
545                 550                 555                 560 

Lys Ala Thr Ser Leu Pro Ser Ser Val Ala Pro Asp Gly Ala Gly His 
                565                 570                 575     

Lys Glu Met Asp Asp Phe Val Ala Val Thr Glu Ala Val Glu Gly Val 
            580                 585                 590         

Asp Val 
        


<210> 11
<211> 2129
<212> DNA
<213> Panicum virgatum

<220>
<221> misc_feature
<223> Ceres CLONE ID no. 1806436 

<220>
<221> misc_feature
<223> The DNA sequence encodes the protein given in SEQ ID NO. 12 

<400> 11
aagcaagctc gcagcgcaaa cgttcctctc ctcctcgtcc tctgttgcct gctgccgccc  60    

tgcacttact tgcagagcaa tcgagctaca cgcagcagct gcaggacagg acagccacca  120   

cgctgcgcca ccagcaatgg tttctgccgg ggggcatggc ggcgacggcg acgacgggca  180   

gaccgtggac ttccggggca acccggccga caagtcccgg accggcgggt ggctcgccgc  240   

cgggctgatc ctgggcacgg agctggcgga gcgcgtgtgc gtgatgggca tttccatgaa  300   

cctcgtcacg tacctcgtcg gcgacctcca cctctccaac tcccggtccg ccaacgtcgt  360   

caccaacttc atgggcacgc tcaacgtgct cgccctcgtc ggcggcttcc tcgccgacgc  420   

caagctcggc cgcttcctca ccatcgccat ctccgccacc atcgccgcca ccggcgtggg  480   

cttgctggcg gtgtgcacgg cggtgccggg catgaggccg ccgccgtgcg ccgacgcgcg  540   

cggcccgcgc gggcacgagt gcgcgccggc gcgcggcggg cagctggcgc tgctgtacgc  600   

ggcgctgtac acgacggcgg cgggcgcggg ggggctcaag gcgaacgtgt ccgggttcgg  660   

gtcggaccag ttcgacgggc gcgacccgcg ggaggagcgc gccatggtct tcttcttcaa  720   

ccgcttctac ctgtgcgtca gcatggggtc gctgctcgcg gtgaccgtgc tggtgtacgt  780   

gcaggacaac gtgggccgcg cctggggcta cggcgtctcc gccgcggcca tgctgctcgc  840   

cgtcgcggtg ctcgccgccg gcgcgcccag gtaccggtac cgccgcccgc ggggcagccc  900   

gctggcgacg atcgggcggg tgctcgccgc agcctggagg aagcgccggc tgccgctccc  960   

cgccgacgcc gccgagctcc acggcttcca cgccgccacg gtcgcccaca ctgacaggct  1020  

gaggtgcctc gacaaggcgg cgatcgtgga ggcggacctg cccgccgcgg cggcggagaa  1080  

ggggccggcg gcggcgccga cggtgacgga ggtggaggag gtgaagatgg tggtgaagct  1140  

gctgcccatc tggtccacgt gcatcctctt ctggacagtc tactcccaga tgaccacctt  1200  

ctccgtggag caggccacgc gcatggaccg ccacctccgc ccgggcggcg ccgccgcctc  1260  

cgggttcgcc gtcccggcgg ggtccttctc cgtcttcctc ttcctctcca tcctgctctt  1320  

cacctccctc aacgagcgcc tcctggtgcc gctcgccgcc cgcctcacgg gccgcccgca  1380  

ggggctcacc tcgctgcagc gcgtcggcgc cggcctcgtc ctctccgtcg ccgccatggc  1440  

cgtcgccgcg ctcgtcgagc ggaggcgccg cgaggcctcc gccggggagg ggcacgtcgc  1500  

catcagcgcc ttctggctcg tgccgcagtt cttcctggtg ggcgccggcg aggcgttcgc  1560  

gtacgtgggg cagctggagt tcttcatccg cgaggcgccc gagcggatga agtccatgag  1620  

cacgggcctc ttcctggtga cgctcgccat gggcttcttc ctcagcagct tcctcgtctt  1680  

cgccgtcgac ggcgccacgc ggggcgcgtg gatccggaac gacctggacc gcgggcggct  1740  

cgacctcttc tactggatgc tggccgtgct cggcgtcgcc aacttcgccg tgttcctcgt  1800  

cttcgccagg cgccaccagt acaaacccag cgccgccgtg ccggccgcgg tggcgcccgc  1860  

cggcagtccg ctggacagcg ccggctccgg ggagaaggag atggacgacc tcgtcgcggt  1920  

caaagaggcc gtggaagggg tggacgtgta gtactactag ggacgacgcg agtagggccc  1980  

gcatgtcaga ctcggaaccg agcggtgtgc tttcgctatt gggtagtgta cgacattttt  2040  

ttttctttcg ctttctcagt ttgctgcttc ggcctgctct cctgtaaata actgtggaga  2100  

catacgaatt gaggcgaagt tttgtttct                                    2129  


<210> 12
<211> 604
<212> PRT
<213> Panicum virgatum

<220>
<221> misc_feature
<223> Ceres CLONE ID no. 1806436 

<220>
<221> misc_feature
<222> (100)..(503)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of NRT 1.3 
      at SEQ ID NO. 2 
      with e-value of 7.10E-187 
      and BLAST sequence identity of 65.0% 

<220>
<221> misc_feature
<223> Bit score of 1450.8 for HMM based on sequences of FIGURE 11 

<400> 12
Met Val Ser Ala Gly Gly His Gly Gly Asp Gly Asp Asp Gly Gln Thr 
1               5                   10                  15      

Val Asp Phe Arg Gly Asn Pro Ala Asp Lys Ser Arg Thr Gly Gly Trp 
            20                  25                  30          

Leu Ala Ala Gly Leu Ile Leu Gly Thr Glu Leu Ala Glu Arg Val Cys 
        35                  40                  45              

Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly Asp Leu 
    50                  55                  60                  

His Leu Ser Asn Ser Arg Ser Ala Asn Val Val Thr Asn Phe Met Gly 
65                  70                  75                  80  

Thr Leu Asn Val Leu Ala Leu Val Gly Gly Phe Leu Ala Asp Ala Lys 
                85                  90                  95      

Leu Gly Arg Phe Leu Thr Ile Ala Ile Ser Ala Thr Ile Ala Ala Thr 
            100                 105                 110         

Gly Val Gly Leu Leu Ala Val Cys Thr Ala Val Pro Gly Met Arg Pro 
        115                 120                 125             

Pro Pro Cys Ala Asp Ala Arg Gly Pro Arg Gly His Glu Cys Ala Pro 
    130                 135                 140                 

Ala Arg Gly Gly Gln Leu Ala Leu Leu Tyr Ala Ala Leu Tyr Thr Thr 
145                 150                 155                 160 

Ala Ala Gly Ala Gly Gly Leu Lys Ala Asn Val Ser Gly Phe Gly Ser 
                165                 170                 175     

Asp Gln Phe Asp Gly Arg Asp Pro Arg Glu Glu Arg Ala Met Val Phe 
            180                 185                 190         

Phe Phe Asn Arg Phe Tyr Leu Cys Val Ser Met Gly Ser Leu Leu Ala 
        195                 200                 205             

Val Thr Val Leu Val Tyr Val Gln Asp Asn Val Gly Arg Ala Trp Gly 
    210                 215                 220                 

Tyr Gly Val Ser Ala Ala Ala Met Leu Leu Ala Val Ala Val Leu Ala 
225                 230                 235                 240 

Ala Gly Ala Pro Arg Tyr Arg Tyr Arg Arg Pro Arg Gly Ser Pro Leu 
                245                 250                 255     

Ala Thr Ile Gly Arg Val Leu Ala Ala Ala Trp Arg Lys Arg Arg Leu 
            260                 265                 270         

Pro Leu Pro Ala Asp Ala Ala Glu Leu His Gly Phe His Ala Ala Thr 
        275                 280                 285             

Val Ala His Thr Asp Arg Leu Arg Cys Leu Asp Lys Ala Ala Ile Val 
    290                 295                 300                 

Glu Ala Asp Leu Pro Ala Ala Ala Ala Glu Lys Gly Pro Ala Ala Ala 
305                 310                 315                 320 

Pro Thr Val Thr Glu Val Glu Glu Val Lys Met Val Val Lys Leu Leu 
                325                 330                 335     

Pro Ile Trp Ser Thr Cys Ile Leu Phe Trp Thr Val Tyr Ser Gln Met 
            340                 345                 350         

Thr Thr Phe Ser Val Glu Gln Ala Thr Arg Met Asp Arg His Leu Arg 
        355                 360                 365             

Pro Gly Gly Ala Ala Ala Ser Gly Phe Ala Val Pro Ala Gly Ser Phe 
    370                 375                 380                 

Ser Val Phe Leu Phe Leu Ser Ile Leu Leu Phe Thr Ser Leu Asn Glu 
385                 390                 395                 400 

Arg Leu Leu Val Pro Leu Ala Ala Arg Leu Thr Gly Arg Pro Gln Gly 
                405                 410                 415     

Leu Thr Ser Leu Gln Arg Val Gly Ala Gly Leu Val Leu Ser Val Ala 
            420                 425                 430         

Ala Met Ala Val Ala Ala Leu Val Glu Arg Arg Arg Arg Glu Ala Ser 
        435                 440                 445             

Ala Gly Glu Gly His Val Ala Ile Ser Ala Phe Trp Leu Val Pro Gln 
    450                 455                 460                 

Phe Phe Leu Val Gly Ala Gly Glu Ala Phe Ala Tyr Val Gly Gln Leu 
465                 470                 475                 480 

Glu Phe Phe Ile Arg Glu Ala Pro Glu Arg Met Lys Ser Met Ser Thr 
                485                 490                 495     

Gly Leu Phe Leu Val Thr Leu Ala Met Gly Phe Phe Leu Ser Ser Phe 
            500                 505                 510         

Leu Val Phe Ala Val Asp Gly Ala Thr Arg Gly Ala Trp Ile Arg Asn 
        515                 520                 525             

Asp Leu Asp Arg Gly Arg Leu Asp Leu Phe Tyr Trp Met Leu Ala Val 
    530                 535                 540                 

Leu Gly Val Ala Asn Phe Ala Val Phe Leu Val Phe Ala Arg Arg His 
545                 550                 555                 560 

Gln Tyr Lys Pro Ser Ala Ala Val Pro Ala Ala Val Ala Pro Ala Gly 
                565                 570                 575     

Ser Pro Leu Asp Ser Ala Gly Ser Gly Glu Lys Glu Met Asp Asp Leu 
            580                 585                 590         

Val Ala Val Lys Glu Ala Val Glu Gly Val Asp Val 
        595                 600                 


<210> 13
<211> 590
<212> PRT
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> Public GI ID no. 15233123 

<220>
<221> misc_feature
<222> (101)..(498)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of  NRT 1.3 
      at SEQ ID NO. 2 
      with e-value of 5.10E-312 
      and BLAST sequence identity of 99.8% 

<220>
<221> misc_feature
<223> Bit score of 1462 for HMM based on sequences of FIGURE 11 

<400> 13
Met Val His Val Ser Ser Ser His Gly Ala Lys Asp Gly Ser Glu Glu 
1               5                   10                  15      

Ala Tyr Asp Tyr Arg Gly Asn Pro Pro Asp Lys Ser Lys Thr Gly Gly 
            20                  25                  30          

Trp Leu Gly Ala Gly Leu Ile Leu Gly Ser Glu Leu Ser Glu Arg Ile 
        35                  40                  45              

Cys Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly Asp 
    50                  55                  60                  

Leu His Ile Ser Ser Ala Lys Ser Ala Thr Ile Val Thr Asn Phe Met 
65                  70                  75                  80  

Gly Thr Leu Asn Leu Leu Gly Leu Leu Gly Gly Phe Leu Ala Asp Ala 
                85                  90                  95      

Lys Leu Gly Arg Tyr Lys Met Val Ala Ile Ser Ala Ser Val Thr Ala 
            100                 105                 110         

Leu Gly Val Leu Leu Leu Thr Val Ala Thr Thr Ile Ser Ser Met Arg 
        115                 120                 125             

Pro Pro Ile Cys Asp Asp Phe Arg Arg Leu His His Gln Cys Ile Glu 
    130                 135                 140                 

Ala Asn Gly His Gln Leu Ala Leu Leu Tyr Val Ala Leu Tyr Thr Ile 
145                 150                 155                 160 

Ala Leu Gly Gly Gly Gly Ile Lys Ser Asn Val Ser Gly Phe Gly Ser 
                165                 170                 175     

Asp Gln Phe Asp Thr Ser Asp Pro Lys Glu Glu Lys Gln Met Ile Phe 
            180                 185                 190         

Phe Phe Asn Arg Phe Tyr Phe Ser Ile Ser Val Gly Ser Leu Phe Ala 
        195                 200                 205             

Val Ile Ala Leu Val Tyr Val Gln Asp Asn Val Gly Arg Gly Trp Gly 
    210                 215                 220                 

Tyr Gly Ile Ser Ala Ala Thr Met Val Val Ala Ala Ile Val Leu Leu 
225                 230                 235                 240 

Cys Gly Thr Lys Arg Tyr Arg Phe Lys Lys Pro Lys Gly Ser Pro Phe 
                245                 250                 255     

Thr Thr Ile Trp Arg Val Gly Phe Leu Ala Trp Lys Lys Arg Lys Glu 
            260                 265                 270         

Ser Tyr Pro Ala His Pro Ser Leu Leu Asn Gly Tyr Asp Asn Thr Thr 
        275                 280                 285             

Val Pro His Thr Glu Met Leu Lys Cys Leu Asp Lys Ala Ala Ile Ser 
    290                 295                 300                 

Lys Asn Glu Ser Ser Pro Ser Ser Lys Asp Phe Glu Glu Lys Asp Pro 
305                 310                 315                 320 

Trp Ile Val Ser Thr Val Thr Gln Val Glu Glu Val Lys Leu Val Met 
                325                 330                 335     

Lys Leu Val Pro Ile Trp Ala Thr Asn Ile Leu Phe Trp Thr Ile Tyr 
            340                 345                 350         

Ser Gln Met Thr Thr Phe Thr Val Glu Gln Ala Thr Phe Met Asp Arg 
        355                 360                 365             

Lys Leu Gly Ser Phe Thr Val Pro Ala Gly Ser Tyr Ser Ala Phe Leu 
    370                 375                 380                 

Ile Leu Thr Ile Leu Leu Phe Thr Ser Leu Asn Glu Arg Val Phe Val 
385                 390                 395                 400 

Pro Leu Thr Arg Arg Leu Thr Lys Lys Pro Gln Gly Ile Thr Ser Leu 
                405                 410                 415     

Gln Arg Ile Gly Val Gly Leu Val Phe Ser Met Ala Ala Met Ala Val 
            420                 425                 430         

Ala Ala Val Ile Glu Asn Ala Arg Arg Glu Ala Ala Val Asn Asn Asp 
        435                 440                 445             

Lys Lys Ile Ser Ala Phe Trp Leu Val Pro Gln Tyr Phe Leu Val Gly 
    450                 455                 460                 

Ala Gly Glu Ala Phe Ala Tyr Val Gly Gln Leu Glu Phe Phe Ile Arg 
465                 470                 475                 480 

Glu Ala Pro Glu Arg Met Lys Ser Met Ser Thr Gly Leu Phe Leu Ser 
                485                 490                 495     

Thr Ile Ser Met Gly Phe Phe Val Ser Ser Leu Leu Val Ser Leu Val 
            500                 505                 510         

Asp Arg Val Thr Asp Lys Ser Trp Leu Arg Ser Asn Leu Asn Lys Ala 
        515                 520                 525             

Arg Leu Asn Tyr Phe Tyr Trp Leu Leu Val Val Leu Gly Ala Leu Asn 
    530                 535                 540                 

Phe Leu Ile Phe Ile Val Phe Ala Met Lys His Gln Tyr Lys Ala Asp 
545                 550                 555                 560 

Val Ile Thr Val Val Val Thr Asp Asp Asp Ser Val Glu Lys Glu Val 
                565                 570                 575     

Thr Lys Lys Glu Ser Ser Glu Phe Glu Leu Lys Asp Ile Pro 
            580                 585                 590 


<210> 14
<211> 590
<212> PRT
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> Public GI ID no. 21536775 

<220>
<221> misc_feature
<222> (101)..(498)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of  NRT 1.3 
      at SEQ ID NO. 2 
      with e-value of 1.70E-311 
      and BLAST sequence identity of 99.6% 

<220>
<221> misc_feature
<223> Bit score of 1458.2 for HMM based on sequences of FIGURE 11 

<400> 14
Met Val His Val Ser Ser Ser His Gly Ala Lys Asp Gly Ser Glu Glu 
1               5                   10                  15      

Ser Phe Asp Tyr Arg Gly Asn Pro Pro Asp Lys Ser Lys Thr Gly Gly 
            20                  25                  30          

Trp Leu Gly Ala Gly Leu Ile Leu Gly Ser Glu Leu Ser Glu Arg Ile 
        35                  40                  45              

Cys Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly Asp 
    50                  55                  60                  

Leu His Ile Ser Ser Ala Lys Ser Ala Thr Ile Val Thr Asn Phe Met 
65                  70                  75                  80  

Gly Thr Leu Asn Leu Leu Gly Leu Leu Gly Gly Phe Leu Ala Asp Ala 
                85                  90                  95      

Lys Leu Gly Arg Tyr Lys Met Val Ala Ile Ser Ala Ser Val Thr Ala 
            100                 105                 110         

Leu Gly Val Leu Leu Leu Thr Val Ala Thr Thr Ile Ser Ser Met Arg 
        115                 120                 125             

Pro Pro Ile Cys Asp Asp Phe Arg Arg Leu His His Gln Cys Ile Glu 
    130                 135                 140                 

Ala Asn Gly His Gln Leu Ala Leu Leu Tyr Val Ala Leu Tyr Thr Ile 
145                 150                 155                 160 

Ala Leu Gly Gly Gly Gly Ile Lys Ser Asn Val Ser Gly Phe Gly Ser 
                165                 170                 175     

Asp Gln Phe Asp Thr Ser Asp Pro Lys Glu Glu Lys Gln Met Ile Phe 
            180                 185                 190         

Phe Phe Asn Arg Phe Tyr Phe Ser Ile Ser Val Gly Ser Leu Phe Ala 
        195                 200                 205             

Val Ile Ala Leu Val Tyr Val Gln Asp Asn Val Gly Arg Gly Trp Gly 
    210                 215                 220                 

Tyr Gly Ile Ser Ala Ala Thr Met Val Val Ala Ala Ile Val Leu Leu 
225                 230                 235                 240 

Cys Gly Thr Lys Arg Tyr Arg Phe Lys Lys Pro Lys Gly Ser Pro Phe 
                245                 250                 255     

Thr Thr Ile Trp Arg Val Gly Phe Leu Ala Trp Lys Lys Arg Lys Glu 
            260                 265                 270         

Ser Tyr Pro Ala His Pro Ser Leu Leu Asn Gly Tyr Asp Asn Thr Thr 
        275                 280                 285             

Val Pro His Thr Glu Met Leu Lys Cys Leu Asn Lys Ala Ala Ile Ser 
    290                 295                 300                 

Lys Asn Glu Ser Ser Pro Ser Ser Lys Asp Phe Glu Glu Lys Asp Pro 
305                 310                 315                 320 

Trp Ile Val Ser Thr Val Thr Gln Val Glu Glu Val Lys Leu Val Met 
                325                 330                 335     

Lys Leu Val Pro Ile Trp Ala Thr Asn Ile Leu Phe Trp Thr Ile Tyr 
            340                 345                 350         

Ser Gln Met Thr Thr Phe Thr Val Glu Gln Ala Thr Phe Met Asp Arg 
        355                 360                 365             

Lys Leu Gly Ser Phe Thr Val Pro Ala Gly Ser Tyr Ser Ala Phe Leu 
    370                 375                 380                 

Ile Leu Thr Ile Leu Leu Phe Thr Ser Leu Asn Glu Arg Val Phe Val 
385                 390                 395                 400 

Pro Leu Thr Arg Arg Leu Thr Lys Lys Pro Gln Gly Ile Thr Ser Leu 
                405                 410                 415     

Gln Arg Ile Gly Val Gly Leu Val Phe Ser Met Ala Ala Met Ala Val 
            420                 425                 430         

Ala Ala Val Ile Glu Asn Ala Arg Arg Glu Ala Ala Val Asn Asn Asp 
        435                 440                 445             

Lys Lys Ile Ser Ala Phe Trp Leu Val Pro Gln Tyr Phe Leu Val Gly 
    450                 455                 460                 

Ala Gly Glu Ala Phe Ala Tyr Val Gly Gln Leu Glu Phe Phe Ile Arg 
465                 470                 475                 480 

Glu Ala Pro Glu Arg Met Lys Ser Met Ser Thr Gly Leu Phe Leu Ser 
                485                 490                 495     

Thr Ile Ser Met Gly Phe Phe Val Ser Ser Leu Leu Val Ser Leu Val 
            500                 505                 510         

Asp Arg Val Thr Asp Lys Ser Trp Leu Arg Ser Asn Leu Asn Lys Ala 
        515                 520                 525             

Arg Leu Asn Tyr Phe Tyr Trp Leu Leu Val Val Leu Gly Ala Leu Asn 
    530                 535                 540                 

Phe Leu Ile Phe Ile Val Phe Ala Met Lys His Gln Tyr Lys Ala Asp 
545                 550                 555                 560 

Val Ile Thr Val Val Val Thr Asp Asp Asp Ser Val Glu Lys Glu Val 
                565                 570                 575     

Thr Lys Lys Glu Ser Ser Glu Phe Glu Leu Lys Asp Ile Pro 
            580                 585                 590 


<210> 15
<211> 592
<212> PRT
<213> Oryza sativa subsp. japonica

<220>
<221> misc_feature
<223> Public GI ID no. 21741984 

<220>
<221> misc_feature
<222> (98)..(503)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of  NRT 1.3 
      at SEQ ID NO. 2 
      with e-value of 3.29E-182 
      and BLAST sequence identity of 61.3% 

<220>
<221> misc_feature
<223> Bit score of 1218.9 for HMM based on sequences of FIGURE 11 

<400> 15
Met Val Ser Ala Gly Val His Gly Gly Asp Asp Gly Val Val Val Asp 
1               5                   10                  15      

Phe Arg Gly Asn Pro Val Asp Lys Asp Arg Thr Gly Gly Trp Leu Gly 
            20                  25                  30          

Ala Gly Leu Ile Leu Gly Thr Glu Leu Ala Glu Arg Val Cys Val Val 
        35                  40                  45              

Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly Asp Leu His Leu 
    50                  55                  60                  

Ser Asn Ala Arg Ser Ala Asn Ile Val Thr Asn Phe Leu Gly Thr Leu 
65                  70                  75                  80  

Asn Leu Leu Ala Leu Leu Gly Gly Phe Leu Ala Asp Ala Val Leu Gly 
                85                  90                  95      

Arg Tyr Leu Thr Val Ala Val Ser Ala Thr Ile Ala Ala Ile Gly Val 
            100                 105                 110         

Ser Leu Leu Ala Ala Ser Thr Val Val Pro Gly Met Arg Pro Pro Pro 
        115                 120                 125             

Cys Gly Asp Ala Val Ala Ala Ala Ala Ala Ala Glu Ser Gly Gly Cys 
    130                 135                 140                 

Val Ala Ala Ser Gly Gly Gln Met Ala Met Leu Tyr Ala Ala Leu Tyr 
145                 150                 155                 160 

Thr Ala Ala Ala Gly Ala Gly Gly Leu Lys Ala Asn Val Ser Gly Phe 
                165                 170                 175     

Gly Ser Asp Gln Phe Asp Gly Arg Asp Arg Arg Glu Gly Lys Ala Met 
            180                 185                 190         

Leu Phe Phe Phe Asn Arg Phe Tyr Phe Cys Ile Ser Leu Gly Ser Val 
        195                 200                 205             

Leu Ala Val Thr Ala Leu Val Tyr Val Gln Glu Asp Val Gly Arg Gly 
    210                 215                 220                 

Trp Gly Tyr Gly Ala Ser Ala Ala Ala Met Val Ala Ala Val Ala Val 
225                 230                 235                 240 

Phe Ala Ala Gly Thr Pro Arg Tyr Arg Tyr Arg Arg Pro Gln Gly Ser 
                245                 250                 255     

Pro Leu Thr Ala Ile Gly Arg Val Leu Trp Ala Ala Trp Arg Lys Arg 
            260                 265                 270         

Arg Met Pro Phe Pro Ala Asp Ala Gly Glu Leu His Gly Phe His Lys 
        275                 280                 285             

Ala Lys Val Pro His Thr Asn Arg Leu Arg Cys Leu Asp Lys Ala Ala 
    290                 295                 300                 

Ile Val Glu Ala Asp Leu Ala Ala Ala Thr Pro Pro Glu Gln Pro Val 
305                 310                 315                 320 

Ala Ala Leu Thr Val Thr Glu Val Glu Glu Ala Lys Met Val Val Lys 
                325                 330                 335     

Leu Leu Pro Ile Trp Ser Thr Ser Ile Leu Phe Trp Thr Val Tyr Ser 
            340                 345                 350         

Gln Met Thr Thr Phe Ser Val Glu Gln Ala Ser His Met Asp Arg Arg 
        355                 360                 365             

Ala Gly Gly Phe Ala Val Pro Ala Gly Ser Phe Ser Val Phe Leu Phe 
    370                 375                 380                 

Leu Ser Ile Leu Leu Phe Thr Ser Ala Ser Glu Arg Leu Leu Val Pro 
385                 390                 395                 400 

Leu Ala Arg Arg Leu Met Ile Thr Arg Arg Pro Gln Gly Leu Thr Ser 
                405                 410                 415     

Leu Gln Arg Val Gly Ala Gly Leu Val Leu Ala Thr Leu Ala Met Ala 
            420                 425                 430         

Val Ser Ala Leu Val Glu Lys Lys Arg Arg Asp Ala Ser Gly Gly Ala 
        435                 440                 445             

Gly Gly Gly Gly Val Ala Met Ile Ser Ala Phe Trp Leu Val Pro Gln 
    450                 455                 460                 

Phe Phe Leu Val Gly Ala Gly Glu Ala Phe Ala Tyr Val Gly Gln Leu 
465                 470                 475                 480 

Glu Phe Phe Ile Arg Glu Ala Pro Glu Arg Met Lys Ser Met Ser Thr 
                485                 490                 495     

Gly Leu Phe Leu Ala Thr Leu Ala Met Gly Phe Phe Leu Ser Ser Leu 
            500                 505                 510         

Leu Val Ser Ala Val Asp Ala Ala Thr Arg Gly Ala Trp Ile Arg Asp 
        515                 520                 525             

Gly Leu Asp Asp Gly Arg Leu Asp Leu Phe Tyr Trp Met Leu Ala Ala 
    530                 535                 540                 

Leu Gly Val Ala Asn Phe Ala Ala Phe Leu Val Phe Ala Ser Arg His 
545                 550                 555                 560 

Gln Tyr Arg Pro Ala Ile Leu Pro Ala Ala Asp Ser Pro Pro Asp Asp 
                565                 570                 575     

Glu Gly Ala Val Arg Glu Ala Ala Thr Thr Val Lys Gly Met Asp Phe 
            580                 585                 590         


<210> 16
<211> 609
<212> PRT
<213> Oryza sativa subsp. japonica

<220>
<221> misc_feature
<223> Public GI ID no. 115446921 

<220>
<221> misc_feature
<222> (101)..(508)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of  NRT 1.3 
      at SEQ ID NO. 2 
      with e-value of 2.90E-190 
      and BLAST sequence identity of 63.4% 

<220>
<221> misc_feature
<223> Bit score of 1462.8 for HMM based on sequences of FIGURE 11 

<400> 16
Met Val Ala Ala Gly Gly Gly His Gly Gly Gly Ala Asp Asp Gly Met 
1               5                   10                  15      

Val Val Asp Tyr Arg Gly Asn Pro Val Asp Lys Ser Lys Thr Gly Gly 
            20                  25                  30          

Trp Leu Gly Ala Gly Leu Ile Leu Gly Thr Glu Leu Ala Glu Arg Val 
        35                  40                  45              

Cys Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly Asp 
    50                  55                  60                  

Leu His Leu Ser Asn Ala Lys Ser Ala Asn Ile Val Thr Asn Phe Met 
65                  70                  75                  80  

Gly Thr Leu Asn Leu Leu Ala Leu Val Gly Gly Phe Leu Ala Asp Ala 
                85                  90                  95      

Lys Leu Gly Arg Tyr Leu Thr Ile Ala Ile Ser Ala Thr Ile Ala Ala 
            100                 105                 110         

Thr Gly Val Ser Leu Leu Thr Val Asp Thr Met Val Pro Gly Met Arg 
        115                 120                 125             

Pro Pro Pro Cys Ala Asp Ala Arg Gly Ala Gly Ala His Leu Arg Cys 
    130                 135                 140                 

Glu Pro Ala Arg Gly Gly Gln Leu Ala Met Leu Tyr Ala Ala Leu Tyr 
145                 150                 155                 160 

Thr Val Ala Ala Gly Ala Gly Gly Leu Lys Ala Asn Val Ser Gly Phe 
                165                 170                 175     

Gly Ser Asp Gln Phe Asp Gly Gly Asp Pro Arg Glu Glu Arg Ala Met 
            180                 185                 190         

Val Phe Phe Phe Asn Arg Phe Tyr Phe Cys Ile Ser Leu Gly Ser Leu 
        195                 200                 205             

Phe Ala Val Thr Val Leu Val Tyr Val Gln Asp Asn Val Gly Arg Gly 
    210                 215                 220                 

Trp Gly Tyr Gly Val Ser Ala Val Ala Met Val Leu Ala Val Ala Val 
225                 230                 235                 240 

Leu Val Ala Gly Thr Pro Lys Tyr Arg Tyr Arg Arg Pro Glu Gly Ser 
                245                 250                 255     

Pro Leu Thr Val Ile Gly Arg Val Leu Ala Thr Ala Trp Arg Lys Arg 
            260                 265                 270         

Arg Leu Pro Leu Pro Ala Asp Ala Gly Glu Leu His Gly Tyr His Thr 
        275                 280                 285             

Ser Lys Val Ala Tyr Thr Asp Arg Leu Arg Cys Leu Asp Arg Ala Ala 
    290                 295                 300                 

Ile Met Glu Ala Asp Leu Ala Ala Ser Pro Ala Lys Thr Asn Gln Thr 
305                 310                 315                 320 

Ser Ala Ala Pro Ala Ala Thr Val Thr Glu Val Glu Glu Val Lys Met 
                325                 330                 335     

Val Val Lys Leu Leu Pro Ile Trp Ser Thr Cys Ile Leu Phe Trp Thr 
            340                 345                 350         

Val Tyr Ser Gln Met Thr Thr Phe Ser Val Glu Gln Ala Thr Arg Met 
        355                 360                 365             

Asp Arg His Leu Arg Pro Gly Ala Ala Pro Gly Gly Phe Ala Ile Pro 
    370                 375                 380                 

Ala Gly Ser Leu Ser Val Phe Leu Phe Leu Ser Ile Leu Leu Phe Thr 
385                 390                 395                 400 

Ser Leu Asn Glu Arg Val Leu Val Pro Ala Ala Arg Arg Leu Thr Arg 
                405                 410                 415     

Arg Pro Gln Gly Leu Thr Ser Leu Gln Arg Val Gly Ala Gly Leu Val 
            420                 425                 430         

Leu Ala Thr Val Ala Met Ala Thr Ser Ala Leu Val Glu Lys Lys Arg 
        435                 440                 445             

Arg Asp Ala Ala Asn Asp Gly Gly Gly Gly Gly Met Ile Ser Ala Phe 
    450                 455                 460                 

Trp Leu Val Pro Gln Phe Phe Leu Val Gly Ala Gly Glu Ala Phe Ala 
465                 470                 475                 480 

Tyr Val Gly Gln Leu Glu Phe Phe Ile Arg Glu Ala Pro Glu Arg Met 
                485                 490                 495     

Lys Ser Met Ser Thr Gly Leu Phe Leu Val Thr Leu Ser Met Gly Phe 
            500                 505                 510         

Phe Leu Ser Ser Phe Leu Val Phe Ala Val Asp Ala Ala Thr Arg Gly 
        515                 520                 525             

Ala Trp Ile Arg Asn Asp Leu Asp Ala Gly Arg Leu Asp Leu Phe Tyr 
    530                 535                 540                 

Trp Met Leu Ala Val Leu Gly Val Ala Asn Phe Ala Val Phe Leu Val 
545                 550                 555                 560 

Phe Ala Arg Arg His Glu Tyr Lys Gln Pro Ala Gly Thr Ala Ala Val 
                565                 570                 575     

Val Val Ala Pro Ala Ala Ala Lys Asp Gly Gly Ala Gly Glu Glu Lys 
            580                 585                 590         

Glu Met Asp Asp Phe Val Val Val Lys Glu Ala Val Glu Gly Met Asp 
        595                 600                 605             

Val 
    


<210> 17
<211> 577
<212> PRT
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> NRT 1.4 Variant 

<220>
<221> misc_feature
<222> (96)..(494)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Bit score of 1574.2 for HMM based on sequences of FIGURE 12 

<400> 17
Met Glu Ser Lys Gly Ser Trp Thr Val Ala Asp Ala Val Asp Tyr Lys 
1               5                   10                  15      

Gly Arg Pro Ala Asp Lys Ser Lys Thr Gly Gly Trp Ile Thr Ala Ala 
            20                  25                  30          

Leu Ile Leu Gly Ile Glu Val Val Glu Arg Leu Ser Thr Met Gly Ile 
        35                  40                  45              

Ala Val Asn Leu Val Thr Tyr Leu Met Glu Thr Met His Leu Pro Ser 
    50                  55                  60                  

Ser Thr Ser Ala Asn Ile Val Thr Asp Phe Met Gly Thr Ser Phe Leu 
65                  70                  75                  80  

Leu Cys Leu Leu Gly Gly Phe Leu Ala Asp Ser Phe Leu Gly Arg Phe 
                85                  90                  95      

Lys Thr Ile Gly Ile Phe Ser Thr Ile Gln Ala Leu Gly Thr Gly Ala 
            100                 105                 110         

Leu Ala Val Ala Thr Lys Leu Pro Glu Leu Arg Pro Pro Thr Cys His 
        115                 120                 125             

His Gly Glu Ala Cys Ile Pro Ala Thr Ala Phe Gln Met Thr Ile Leu 
    130                 135                 140                 

Tyr Val Ser Leu Tyr Leu Ile Ala Leu Gly Thr Gly Gly Leu Lys Ser 
145                 150                 155                 160 

Ser Ile Ser Gly Phe Gly Ser Asp Gln Phe Asp Asp Lys Asp Pro Lys 
                165                 170                 175     

Glu Lys Ala His Met Ala Phe Phe Phe Asn Arg Phe Phe Phe Phe Ile 
            180                 185                 190         

Ser Met Gly Thr Leu Leu Ala Val Thr Val Leu Val Tyr Met Gln Asp 
        195                 200                 205             

Glu Val Gly Arg Ser Trp Ala Tyr Gly Ile Cys Thr Val Ser Met Ala 
    210                 215                 220                 

Ile Ala Ile Val Ile Phe Leu Cys Gly Thr Lys Arg Tyr Arg Tyr Lys 
225                 230                 235                 240 

Lys Ser Gln Gly Ser Pro Val Val Gln Ile Phe Gln Val Ile Ala Ala 
                245                 250                 255     

Ala Phe Arg Lys Arg Lys Met Glu Leu Pro Gln Ser Ile Val Tyr Leu 
            260                 265                 270         

Tyr Glu Asp Asn Pro Glu Gly Ile Arg Ile Glu His Thr Asp Gln Phe 
        275                 280                 285             

His Leu Leu Asp Lys Ala Ala Ile Val Ala Glu Gly Asp Phe Glu Gln 
    290                 295                 300                 

Thr Leu Asp Gly Val Ala Ile Pro Asn Pro Trp Lys Leu Ser Ser Val 
305                 310                 315                 320 

Thr Lys Val Glu Glu Val Lys Met Met Val Arg Leu Leu Pro Ile Trp 
                325                 330                 335     

Ala Thr Thr Ile Ile Phe Trp Thr Thr Tyr Ala Gln Met Ile Thr Phe 
            340                 345                 350         

Ser Val Glu Gln Ala Ser Thr Met Arg Arg Asn Ile Gly Ser Phe Lys 
        355                 360                 365             

Ile Pro Ala Gly Ser Leu Thr Val Phe Phe Val Ala Ala Ile Leu Ile 
    370                 375                 380                 

Thr Leu Ala Val Tyr Asp Arg Ala Ile Met Pro Phe Trp Lys Lys Trp 
385                 390                 395                 400 

Lys Gly Lys Pro Gly Phe Ser Ser Leu Gln Arg Ile Ala Ile Gly Leu 
                405                 410                 415     

Val Leu Ser Thr Ala Gly Met Ala Ala Ala Ala Leu Val Glu Gln Lys 
            420                 425                 430         

Arg Leu Ser Val Ala Lys Ser Ser Ser Gln Lys Thr Leu Pro Ile Ser 
        435                 440                 445             

Val Phe Leu Leu Val Pro Gln Phe Phe Leu Val Gly Ala Gly Glu Ala 
    450                 455                 460                 

Phe Ile Tyr Thr Gly Gln Leu Asp Phe Phe Ile Thr Gln Ser Pro Lys 
465                 470                 475                 480 

Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Thr Thr Leu Ser Leu 
                485                 490                 495     

Gly Phe Phe Val Ser Ser Phe Leu Val Ser Ile Val Lys Arg Val Thr 
            500                 505                 510         

Ser Thr Ser Thr Asp Gly Gly Trp Leu Ala Asp Asn Ile Asn His Gly 
        515                 520                 525             

Arg Leu Asp Tyr Phe His Trp Leu Leu Val Ile Leu Ser Gly Ile Asn 
    530                 535                 540                 

Phe Val Val Tyr Ile Ile Cys Ala Leu Trp Phe Lys Pro Thr Lys Gly 
545                 550                 555                 560 

Lys Asp Ser Val Glu Lys Glu Asn Gly Lys Gly Phe Ser Val Glu Asp 
                565                 570                 575     

Cys 
    


<210> 18
<211> 3570
<212> DNA
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> NRT 1.4 Genomic     

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 1314724 

<220>
<221> misc_feature
<223> The DNA sequence encodes the protein given in SEQ ID NO. 20 

<400> 18
atggtttgga taatcttttt cttcttactc gtaaactatt ggttataaga ttcgggaact  60    

ttggattgac gtacgtcgtt gttttcagga gagcaaaggg agttggacag tggctgatgc  120   

cgtagactac aaaggacgac ctgccgacaa atccaaaacc ggtggttgga tcactgccgc  180   

tctcattctt ggttcgtctt cttcttttct acttgacata tatatatata tatatatata  240   

catttggtcc tcatgtacat aatagatatg ccattttttt tgtgtgtgca catatactta  300   

tgccattatc aataagaatc tgtgtgaaat ttattcacag aagtcctagt taattagcta  360   

aaaatgaact ctttttttat agctaaaaac gaacttcttt ttcttcttta tttaactgca  420   

tgctttttta tctttctttt tttactaaaa tcttcgtttc ttacttcatg tgacatgcat  480   

tactactata tatttatatt atttaatatt ctgtaaatga agaaaataca tctatcatcc  540   

acatattggc agagtagtca ttagtgaata catacataca acttatacgt aattaatttg  600   

atcaagaggg tgtagttcat gcgtataagt tttaaattcc ataagttgta taatttgtga  660   

atacatggtg agaaacaaaa gagactcatt ttcaaatcag tcacactcac acatgcatga  720   

tatgattgaa ataaatatat ttgtatataa aaatatgaaa tgtcgttgtt ctgaatctcg  780   

ctgattgaaa gaaagataaa gagaaaagga acacgccaca tggctcccca tctcgcttat  840   

ccaatcataa taacattttt ttttcttttt taaaatttaa tcattctgaa aaacaatatt  900   

gattagaaag agaaaaacga aaactggatt tatatctgta ttcacgtgtc tacttaggga  960   

gcagaaaatg ccgacctcca aacccttgtt ttgttcctta atattctctt acaaaaagta  1020  

ccagttgtct taattaaaat tagtccggat aatccaaaat ttagttttgt tgcttctaag  1080  

tttaaaactg ataatatatc tccttcactt ccttactttt ttaaaggaca aaaagattga  1140  

atgttacatc cataacttat catgaatatt catttatgtt tatcaatact ttatatttta  1200  

ttttgttggc caaattaata gttggagaat gaaaggtcta tgttattttc gtattaattt  1260  

accttaaaac aatcaaactt gttaatcagc ttatggctgc ttactagtta aggattattc  1320  

tacttatatg tatgaaatgg actcaccaat taacttggaa catatgtaca aaactacaaa  1380  

tgttgacaca ttaaaaatga aacatatatt gcagggatag aagttgtgga gaggctatca  1440  

acaatgggaa tagcagtgaa tttggtaaca tatttgatgg agacaatgca tctcccaagt  1500  

tcaacctctg ccaacattgt cactgatttc atgggcactt ccttcctcct atgcttgctc  1560  

ggtggttttc tcgctgactc cttcctcggc cgtttcaaaa ccatcggcat tttctcaacc  1620  

attcaagctc tggtaagact ctatctaaaa tcttatatat agttttaaaa aggtcattgt  1680  

gattagttgg ttttaccatc tagtagtaga tgcgtctaag agaataaata acctgtattt  1740  

ttctccaaga tctccgattc taaaactact attataagca aattatcaat tagagctcta  1800  

ttaaaaaaat tcacttagaa aaaatcaaac aaaatgttcg ctatcaaatc tgctaaggtc  1860  

gttaacttag ttggagtctt ggagacggtt ttaatatctt gtatacaggg aactggtgct  1920  

ctagcggtag caactaagct gccagagcta cgtccaccaa catgccatca tggagaagct  1980  

tgcatacccg cgaccgcctt ccaaatgaca attctttatg tttcgcttta ccttatagcc  2040  

cttggaactg gtggtcttaa atctagtatc tctggatttg ggtctgacca gtttgatgac  2100  

aaagatccta aagagaaagc tcacatggct ttcttcttca acaggttagg tttagagttt  2160  

attttcatgc atggaattat aacattgtta tagtgttccc tttttcaaat aaaattttaa  2220  

ttggcacatg caggttcttc ttctttatta gtatggggac attattggct gtgactgttt  2280  

tagtttacat gcaagatgaa gtgggaagat cttgggctta tggaatctgc actgtctcta  2340  

tggctatagc tattgtaata ttcttgtgtg ggactaagag ataccgttat aagaagagcc  2400  

aaggaagtcc cgttgtgcaa atatttcagg tcatagcagc tgcgttccga aagaggaaaa  2460  

tggaactacc tcaaagcatt gtttatcttt atgaagataa ccctgaaggc attagaattg  2520  

aacatactga tcagtttcag tgagtttctc agttcaatct ttacaaagag gaagtaaatt  2580  

aaagtaaatt ttgtatctaa taaaatcttg tgtgtagctt gttggacaag gcggccatag  2640  

ttgcagaagg agattttgaa caaacccttg atggagttgc aatcccaaac ccttggaagc  2700  

taagctcagt gaccaaagtt gaggaagtaa aaatgatggt taggcttttg cctatttggg  2760  

caacaactat aattttttgg acaacatatg cccaaatgat tacattctct gttgagcaag  2820  

cttcaactat gagacgtaac attggaagct ttaagatccc agctggttcc ctcaccgtgt  2880  

ttttcgttgc ggctattctc ataactctag ctgtctacga ccgtgccata atgccttttt  2940  

ggaagaaatg gaaaggaaaa ccaggtgaca taagattcat agattcatat atctattctt  3000  

ttttcatata tgtctctcag ctatatattc acatgcatta acatttcagg tttctctagc  3060  

ctacaaagaa tagctattgg attggtctta tcaaccgctg gaatggcagc tgcagctcta  3120  

gtagagcaaa agcgtttatc cgttgcgaaa tctagttcac aaaaaacatt gcctataagt  3180  

gtgtttttac ttgttccaca attcttctta gtaggagctg gggaagcctt tatctacact  3240  

ggccaacttg atttcttcat aacacaatcg cctaagggaa tgaaaactat gagcactgga  3300  

ctcttcttga ccactttatc actaggtttc tttgtcagca gtttcttggt ctcaatcgtc  3360  

aagagggtca cttcaacttc tactgatgta ggatggctgg ctgataacat taaccacggc  3420  

cgactcgatt acttttattg gcttttagtc attctcagtg gaattaactt cgttgtctat  3480  

atcatatgtg ccttgtggtt taagccaacg aagggtaaag actcagtaga gaaggaaaat  3540  

ggcaagggat tttcagttga agactgctga                                   3570  


<210> 19
<211> 1734
<212> DNA
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> NRT 1.4 Predicted cDNA Sequence 

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 1314724 

<220>
<221> misc_feature
<223> The DNA sequence encodes the protein given in SEQ ID NO. 20 

<400> 19
atggagagca aagggagttg gacagtggct gatgccgtag actacaaagg acgacctgcc  60    

gacaaatcca aaaccggtgg ttggatcact gccgctctca ttcttgggat agaagttgtg  120   

gagaggctat caacaatggg aatagcagtg aatttggtaa catatttgat ggagacaatg  180   

catctcccaa gttcaacctc tgccaacatt gtcactgatt tcatgggcac ttccttcctc  240   

ctatgcttgc tcggtggttt tctcgctgac tccttcctcg gccgtttcaa aaccatcggc  300   

attttctcaa ccattcaagc tctgggaact ggtgctctag cggtagcaac taagctgcca  360   

gagctacgtc caccaacatg ccatcatgga gaagcttgca tacccgcgac cgccttccaa  420   

atgacaattc tttatgtttc gctttacctt atagcccttg gaactggtgg tcttaaatct  480   

agtatctctg gatttgggtc tgaccagttt gatgacaaag atcctaaaga gaaagctcac  540   

atggctttct tcttcaacag gttcttcttc tttattagta tggggacatt attggctgtg  600   

actgttttag tttacatgca agatgaagtg ggaagatctt gggcttatgg aatctgcact  660   

gtctctatgg ctatagctat tgtaatattc ttgtgtggga ctaagagata ccgttataag  720   

aagagccaag gaagtcccgt tgtgcaaata tttcaggtca tagcagctgc gttccgaaag  780   

aggaaaatgg aactacctca aagcattgtt tatctttatg aagataaccc tgaaggcatt  840   

agaattgaac atactgatca gtttcacttg ttggacaagg cggccatagt tgcagaagga  900   

gattttgaac aaacccttga tggagttgca atcccaaacc cttggaagct aagctcagtg  960   

accaaagttg aggaagtaaa aatgatggtt aggcttttgc ctatttgggc aacaactata  1020  

attttttgga caacatatgc ccaaatgatt acattctctg ttgagcaagc ttcaactatg  1080  

agacgtaaca ttggaagctt taagatccca gctggttccc tcaccgtgtt tttcgttgcg  1140  

gctattctca taactctagc tgtctacgac cgtgccataa tgcctttttg gaagaaatgg  1200  

aaaggaaaac caggtttctc tagcctacaa agaatagcta ttggattggt cttatcaacc  1260  

gctggaatgg cagctgcagc tctagtagag caaaagcgtt tatccgttgc gaaatctagt  1320  

tcacaaaaaa cattgcctat aagtgtgttt ttacttgttc cacaattctt cttagtagga  1380  

gctggggaag cctttatcta cactggccaa cttgatttct tcataacaca atcgcctaag  1440  

ggaatgaaaa ctatgagcac tggactcttc ttgaccactt tatcactagg tttctttgtc  1500  

agcagtttct tggtctcaat cgtcaagagg gtcacttcaa cttctactga tgtaggatgg  1560  

ctggctgata acattaacca cggccgactc gattactttt attggctttt agtcattctc  1620  

agtggaatta acttcgttgt ctatatcata tgtgccttgt ggtttaagcc aacgaagggt  1680  

aaagactcag tagagaagga aaatggcaag ggattttcag ttgaagactg ctga        1734  


<210> 20
<211> 577
<212> PRT
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 1314724 

<220>
<221> misc_feature
<222> (96)..(494)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1574 for HMM based on sequences of FIGURE 12 

<400> 20
Met Glu Ser Lys Gly Ser Trp Thr Val Ala Asp Ala Val Asp Tyr Lys 
1               5                   10                  15      

Gly Arg Pro Ala Asp Lys Ser Lys Thr Gly Gly Trp Ile Thr Ala Ala 
            20                  25                  30          

Leu Ile Leu Gly Ile Glu Val Val Glu Arg Leu Ser Thr Met Gly Ile 
        35                  40                  45              

Ala Val Asn Leu Val Thr Tyr Leu Met Glu Thr Met His Leu Pro Ser 
    50                  55                  60                  

Ser Thr Ser Ala Asn Ile Val Thr Asp Phe Met Gly Thr Ser Phe Leu 
65                  70                  75                  80  

Leu Cys Leu Leu Gly Gly Phe Leu Ala Asp Ser Phe Leu Gly Arg Phe 
                85                  90                  95      

Lys Thr Ile Gly Ile Phe Ser Thr Ile Gln Ala Leu Gly Thr Gly Ala 
            100                 105                 110         

Leu Ala Val Ala Thr Lys Leu Pro Glu Leu Arg Pro Pro Thr Cys His 
        115                 120                 125             

His Gly Glu Ala Cys Ile Pro Ala Thr Ala Phe Gln Met Thr Ile Leu 
    130                 135                 140                 

Tyr Val Ser Leu Tyr Leu Ile Ala Leu Gly Thr Gly Gly Leu Lys Ser 
145                 150                 155                 160 

Ser Ile Ser Gly Phe Gly Ser Asp Gln Phe Asp Asp Lys Asp Pro Lys 
                165                 170                 175     

Glu Lys Ala His Met Ala Phe Phe Phe Asn Arg Phe Phe Phe Phe Ile 
            180                 185                 190         

Ser Met Gly Thr Leu Leu Ala Val Thr Val Leu Val Tyr Met Gln Asp 
        195                 200                 205             

Glu Val Gly Arg Ser Trp Ala Tyr Gly Ile Cys Thr Val Ser Met Ala 
    210                 215                 220                 

Ile Ala Ile Val Ile Phe Leu Cys Gly Thr Lys Arg Tyr Arg Tyr Lys 
225                 230                 235                 240 

Lys Ser Gln Gly Ser Pro Val Val Gln Ile Phe Gln Val Ile Ala Ala 
                245                 250                 255     

Ala Phe Arg Lys Arg Lys Met Glu Leu Pro Gln Ser Ile Val Tyr Leu 
            260                 265                 270         

Tyr Glu Asp Asn Pro Glu Gly Ile Arg Ile Glu His Thr Asp Gln Phe 
        275                 280                 285             

His Leu Leu Asp Lys Ala Ala Ile Val Ala Glu Gly Asp Phe Glu Gln 
    290                 295                 300                 

Thr Leu Asp Gly Val Ala Ile Pro Asn Pro Trp Lys Leu Ser Ser Val 
305                 310                 315                 320 

Thr Lys Val Glu Glu Val Lys Met Met Val Arg Leu Leu Pro Ile Trp 
                325                 330                 335     

Ala Thr Thr Ile Ile Phe Trp Thr Thr Tyr Ala Gln Met Ile Thr Phe 
            340                 345                 350         

Ser Val Glu Gln Ala Ser Thr Met Arg Arg Asn Ile Gly Ser Phe Lys 
        355                 360                 365             

Ile Pro Ala Gly Ser Leu Thr Val Phe Phe Val Ala Ala Ile Leu Ile 
    370                 375                 380                 

Thr Leu Ala Val Tyr Asp Arg Ala Ile Met Pro Phe Trp Lys Lys Trp 
385                 390                 395                 400 

Lys Gly Lys Pro Gly Phe Ser Ser Leu Gln Arg Ile Ala Ile Gly Leu 
                405                 410                 415     

Val Leu Ser Thr Ala Gly Met Ala Ala Ala Ala Leu Val Glu Gln Lys 
            420                 425                 430         

Arg Leu Ser Val Ala Lys Ser Ser Ser Gln Lys Thr Leu Pro Ile Ser 
        435                 440                 445             

Val Phe Leu Leu Val Pro Gln Phe Phe Leu Val Gly Ala Gly Glu Ala 
    450                 455                 460                 

Phe Ile Tyr Thr Gly Gln Leu Asp Phe Phe Ile Thr Gln Ser Pro Lys 
465                 470                 475                 480 

Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Thr Thr Leu Ser Leu 
                485                 490                 495     

Gly Phe Phe Val Ser Ser Phe Leu Val Ser Ile Val Lys Arg Val Thr 
            500                 505                 510         

Ser Thr Ser Thr Asp Val Gly Trp Leu Ala Asp Asn Ile Asn His Gly 
        515                 520                 525             

Arg Leu Asp Tyr Phe Tyr Trp Leu Leu Val Ile Leu Ser Gly Ile Asn 
    530                 535                 540                 

Phe Val Val Tyr Ile Ile Cys Ala Leu Trp Phe Lys Pro Thr Lys Gly 
545                 550                 555                 560 

Lys Asp Ser Val Glu Lys Glu Asn Gly Lys Gly Phe Ser Val Glu Asp 
                565                 570                 575     

Cys 
    


<210> 21
<211> 1752
<212> DNA
<213> Populus balsamifera subsp. trichocarpa

<220>
<221> misc_feature 
<223> Ceres ANNOT ID no. 1447247 

<220>
<221> misc_feature
<223> The DNA sequence encodes the protein given in SEQ ID NO. 22 

<400> 21
atgtctcttc cagaaacaca aggcaagacc ctcccagatg cctgggactg taagggccgc  60    

cctgcggagc ggtccaaaac tggtggctgg accagtgctg ccatgattct aggtggagag  120   

gcaatggaga gactaacaac acttggtatt gctgttaatc tggtgacgta tctgactggt  180   

actatgcatc tgggcaatgc tacctctgcc aacactgtca ccaacttcct tggaacatct  240   

ttcatgcttt gtctgcttgg tggttttatt gctgacacct ttcttggaag gtatctcacc  300   

atcgccatct tcgccaccgt gcaagcaacg ggtgtcacaa tcttgacaat ctccaccgta  360   

gtcccaagcc tcaggccacc aaaatgtgtg gacaacacag attgcatccc tgcgaacgga  420   

gaacaactca cggttcttta tttggccctg tacctcactg ccctcggcac tggaggtcta  480   

aaatcaagcg tctcaggatt tggctccgat caattcgatg acacggaccc aaaagaacga  540   

aaacagatgc tcaatttctt taactggttc ttctttctaa tcagcctagg tgcactttgt  600   

gcagtgacag ttctggttta catacaagat aatttgggga gagaatgggg atatggcata  660   

tgtgcctgtg ctattgtgct aggcctaatt gtgttccttt ctggcacaag gcggtaccgg  720   

ttcaggaaat tggtgggtag tccattgaca cagatagcca ctgtttttgt gagtgcttgg  780   

agaaaaaggc acatggaatt accttcagat gtgtctcttc tttacaacgt tgatgatgtt  840   

gaagagggaa gcaaggagaa gaagcaaaag ctgccacata ccaagcaatt ccgtttcctg  900   

gacaaagcag cgatcaaggg cctagaagtg ggcaaggtaa acaagtggaa tttagcaacc  960   

ttaacagatg ttgaagaagt gaaaatggta attagaatgt tgcccatctg ggctactact  1020  

gtgatatttt ggactgtcta tgcccaaatg accacatttt ctgtgtcaca agccaccacc  1080  

atggaccgtc gcattggaaa atctttccaa atacctgcag catcactgac ggtcttcttt  1140  

gttggtagca ttcttttgac tgttcctgtt tatgaccgaa ttatcgtccc agttgccaga  1200  

agagtgctta agcatccaca agggctaacc ccattaagac gcataggagt cggcttggtt  1260  

ttatccattg ttgccatgat agcagcagca ctcactgaaa agaagaggct gagagccgcg  1320  

agattacatg gtttggctaa tgatccaacg gccgagatcc cactgagtgt gttctggctg  1380  

gtcccacaat tcttttttgt ggggtctggt gaagctttca catatatagg gcagcttgat  1440  

ttcttcctga gagagtgtcc aaaggggatg aagacgatga gcacagggct gtttctgagc  1500  

accctttctc tagggttctt tgtcagctct tcattggtta ctatagtgca caaggtgact  1560  

ataaacaagc catggttagc ggataatcta aaccaaggga ggctccatga tttctactgg  1620  

ttgttggcaa ttttgagtgc tttgaacttt gtcatatatt tgatttgtgc cagatggtat  1680  

gtctacaagg acaagaggct cgccgacgag ggaattgaac tggacgaagt tgatgagccc  1740  

actttccact ga                                                      1752  


<210> 22
<211> 583
<212> PRT
<213> Populus balsamifera subsp. trichocarpa

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 1447247 

<220>
<221> misc_feature
<222> (98)..(502)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1587.3 for HMM based on sequences of FIGURE 12 

<400> 22
Met Ser Leu Pro Glu Thr Gln Gly Lys Thr Leu Pro Asp Ala Trp Asp 
1               5                   10                  15      

Cys Lys Gly Arg Pro Ala Glu Arg Ser Lys Thr Gly Gly Trp Thr Ser 
            20                  25                  30          

Ala Ala Met Ile Leu Gly Gly Glu Ala Met Glu Arg Leu Thr Thr Leu 
        35                  40                  45              

Gly Ile Ala Val Asn Leu Val Thr Tyr Leu Thr Gly Thr Met His Leu 
    50                  55                  60                  

Gly Asn Ala Thr Ser Ala Asn Thr Val Thr Asn Phe Leu Gly Thr Ser 
65                  70                  75                  80  

Phe Met Leu Cys Leu Leu Gly Gly Phe Ile Ala Asp Thr Phe Leu Gly 
                85                  90                  95      

Arg Tyr Leu Thr Ile Ala Ile Phe Ala Thr Val Gln Ala Thr Gly Val 
            100                 105                 110         

Thr Ile Leu Thr Ile Ser Thr Val Val Pro Ser Leu Arg Pro Pro Lys 
        115                 120                 125             

Cys Val Asp Asn Thr Asp Cys Ile Pro Ala Asn Gly Glu Gln Leu Thr 
    130                 135                 140                 

Val Leu Tyr Leu Ala Leu Tyr Leu Thr Ala Leu Gly Thr Gly Gly Leu 
145                 150                 155                 160 

Lys Ser Ser Val Ser Gly Phe Gly Ser Asp Gln Phe Asp Asp Thr Asp 
                165                 170                 175     

Pro Lys Glu Arg Lys Gln Met Leu Asn Phe Phe Asn Trp Phe Phe Phe 
            180                 185                 190         

Leu Ile Ser Leu Gly Ala Leu Cys Ala Val Thr Val Leu Val Tyr Ile 
        195                 200                 205             

Gln Asp Asn Leu Gly Arg Glu Trp Gly Tyr Gly Ile Cys Ala Cys Ala 
    210                 215                 220                 

Ile Val Leu Gly Leu Ile Val Phe Leu Ser Gly Thr Arg Arg Tyr Arg 
225                 230                 235                 240 

Phe Arg Lys Leu Val Gly Ser Pro Leu Thr Gln Ile Ala Thr Val Phe 
                245                 250                 255     

Val Ser Ala Trp Arg Lys Arg His Met Glu Leu Pro Ser Asp Val Ser 
            260                 265                 270         

Leu Leu Tyr Asn Val Asp Asp Val Glu Glu Gly Ser Lys Glu Lys Lys 
        275                 280                 285             

Gln Lys Leu Pro His Thr Lys Gln Phe Arg Phe Leu Asp Lys Ala Ala 
    290                 295                 300                 

Ile Lys Gly Leu Glu Val Gly Lys Val Asn Lys Trp Asn Leu Ala Thr 
305                 310                 315                 320 

Leu Thr Asp Val Glu Glu Val Lys Met Val Ile Arg Met Leu Pro Ile 
                325                 330                 335     

Trp Ala Thr Thr Val Ile Phe Trp Thr Val Tyr Ala Gln Met Thr Thr 
            340                 345                 350         

Phe Ser Val Ser Gln Ala Thr Thr Met Asp Arg Arg Ile Gly Lys Ser 
        355                 360                 365             

Phe Gln Ile Pro Ala Ala Ser Leu Thr Val Phe Phe Val Gly Ser Ile 
    370                 375                 380                 

Leu Leu Thr Val Pro Val Tyr Asp Arg Ile Ile Val Pro Val Ala Arg 
385                 390                 395                 400 

Arg Val Leu Lys His Pro Gln Gly Leu Thr Pro Leu Arg Arg Ile Gly 
                405                 410                 415     

Val Gly Leu Val Leu Ser Ile Val Ala Met Ile Ala Ala Ala Leu Thr 
            420                 425                 430         

Glu Lys Lys Arg Leu Arg Ala Ala Arg Leu His Gly Leu Ala Asn Asp 
        435                 440                 445             

Pro Thr Ala Glu Ile Pro Leu Ser Val Phe Trp Leu Val Pro Gln Phe 
    450                 455                 460                 

Phe Phe Val Gly Ser Gly Glu Ala Phe Thr Tyr Ile Gly Gln Leu Asp 
465                 470                 475                 480 

Phe Phe Leu Arg Glu Cys Pro Lys Gly Met Lys Thr Met Ser Thr Gly 
                485                 490                 495     

Leu Phe Leu Ser Thr Leu Ser Leu Gly Phe Phe Val Ser Ser Ser Leu 
            500                 505                 510         

Val Thr Ile Val His Lys Val Thr Ile Asn Lys Pro Trp Leu Ala Asp 
        515                 520                 525             

Asn Leu Asn Gln Gly Arg Leu His Asp Phe Tyr Trp Leu Leu Ala Ile 
    530                 535                 540                 

Leu Ser Ala Leu Asn Phe Val Ile Tyr Leu Ile Cys Ala Arg Trp Tyr 
545                 550                 555                 560 

Val Tyr Lys Asp Lys Arg Leu Ala Asp Glu Gly Ile Glu Leu Asp Glu 
                565                 570                 575     

Val Asp Glu Pro Thr Phe His 
            580             


<210> 23
<211> 2079
<212> DNA
<213> Panicum virgatum

<220>
<221> misc_feature
<223> Ceres CLONE ID no. 1777083 

<220>
<221> misc_feature
<222> (1)..(2079)
<223> The DNA sequence encodes the protein given in SEQ ID NO. 24 

<400> 23
agcaagcaag cgcagcgttc gatcgctcga gctactacta gctgagccac acgtacgcct  60    

tgtggtgcct tgtgttcttc tgctcggcag ttagctgcac actgttgcta gctagctgat  120   

acagtccaag ctcatggacg ggaagaaggt gcagcaggag cgggcggcgt ggggcgccgg  180   

cgacggccgc ggcaacctgg tgcaggacgc ggtggactac aggggctgcc gggcggacaa  240   

gtccagcacg ggcggctggg tggcggcggc gctggccctc gggatcgagc tctgcgagcg  300   

cctctccacc atgggcatcg ccgtgaacct cgtcacctac ctcacgggca ccatgcacct  360   

gcaaagcgcc gcggccgcca acgtagtcac cgacttcatg ggcacctcct tcctcctctg  420   

cctcctcggc ggcttcctcg ccgactcctt cctcggccgg tacctcacca tcgccatctt  480   

cgccctcgtc caagccatcg gaacggggct cctggcggtg tcgacggagg tgcggcagct  540   

gcggccgccg ccgtgcggac ccggagctgc cggaccgtgc gagcaggcca cggggctcca  600   

gatgggcgtt ctctacgtgt gcctctacct catcgcgctc ggcaccggcg ggctcaagtc  660   

cagcgtctcg ggcttcggca ccgaccagtt cgacgagcgc gacgcccgcg agcgcgccgc  720   

catgggcctc ttcttcaacc gcttcttctt cttcatcagc gtcggcacgc tcctcgccgt  780   

caccgtcctc gtctacgtcc aggaccacgt cggccggagc tgggcgtacg gcatctgcgc  840   

cggcgccatg ctcgtcgccg tcggcgtctt cctctccggc accaggcgct accgctacag  900   

gcgcagctcc gggagcccca tcgtgcacat cctccaggtc ctcgtcgccg ccgcccggaa  960   

gcggggcatc aagcagccgc tcaccgcctc cgcgctctat gaggaccgcc ccgaccacgc  1020  

gcgcatcccc cacaccgccc agttcgcgtg cctggacagg gcggccgtga tggccggcga  1080  

cgacgacaac gaggtgggga gcgacggccg gccggtgccc aacccgtgga agctgtgctc  1140  

cgtgtcccgc gtcgaggagg tgaagatggt ggcgcggctg atgccggtgt gggcgacgac  1200  

gatcctgttc tggaccatct acgcgcagat gatcaccttc tccgtggagc aggcgaccac  1260  

catggaccgg cgcatggacg gcttcgagat cccggccgcc tcgctcaccg tcttcttcgt  1320  

cggcgccatc atgctcactc tcgccgtcta cgaccgcgtg ttcgtgccac tctgccggag  1380  

cctcatgacg ggccggcagg ggctcaccaa cctggagaag atcggcatcg gcctggtcct  1440  

ctccatcgtc ggcatggccg ccgccgccag ctgcgagaag aagcgcctcg ccgtggcagc  1500  

cacgagcgcg gcggcggcgg cggggcacga gccgctgccc atcagcgtgt tcatgctgac  1560  

gccgcagttc ctgctggtgg gcgccggcga ggcgttcatc tacacggggc agctggactt  1620  

cttcatcacg cggtcgccca agggcatgaa gaccatgagc acgggtctct tcctcaccac  1680  

gctctcgctc ggcttcttcc tcagcagcgc gctcgtctcg ctcgtcaagg gctgcacgcg  1740  

gtggctcggc gacaccatca acaacagccg cctcgactac ttctactggc tgctcgccgt  1800  

gctcagcgtc gtcaacctcg tcgcctacct cgtctgcgcc atgtgggcca cgccgccggc  1860  

cagcagccag gcggagcagc cacagatcgc gatgacagcc gacgagaaat gctagcaaca  1920  

tctctgctac agacctctac tgcaccaata taatccccgt tagttataga tttgactatt  1980  

tgagtgtgga tgcagtttat atgctagtta aaaccaggtc acctctgttt actgaaaaat  2040  

aagaaaaagg ctttagatca aaaaaaaaaa aaaaaaaaa                         2079  


<210> 24
<211> 593
<212> PRT
<213> Panicum virgatum

<220>
<221> misc_feature
<223> Ceres CLONE ID no. 1777083 

<220>
<221> misc_feature
<222> (110)..(517)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1567.5 for HMM based on sequences of FIGURE 12 

<400> 24
Met Asp Gly Lys Lys Val Gln Gln Glu Arg Ala Ala Trp Gly Ala Gly 
1               5                   10                  15      

Asp Gly Arg Gly Asn Leu Val Gln Asp Ala Val Asp Tyr Arg Gly Cys 
            20                  25                  30          

Arg Ala Asp Lys Ser Ser Thr Gly Gly Trp Val Ala Ala Ala Leu Ala 
        35                  40                  45              

Leu Gly Ile Glu Leu Cys Glu Arg Leu Ser Thr Met Gly Ile Ala Val 
    50                  55                  60                  

Asn Leu Val Thr Tyr Leu Thr Gly Thr Met His Leu Gln Ser Ala Ala 
65                  70                  75                  80  

Ala Ala Asn Val Val Thr Asp Phe Met Gly Thr Ser Phe Leu Leu Cys 
                85                  90                  95      

Leu Leu Gly Gly Phe Leu Ala Asp Ser Phe Leu Gly Arg Tyr Leu Thr 
            100                 105                 110         

Ile Ala Ile Phe Ala Leu Val Gln Ala Ile Gly Thr Gly Leu Leu Ala 
        115                 120                 125             

Val Ser Thr Glu Val Arg Gln Leu Arg Pro Pro Pro Cys Gly Pro Gly 
    130                 135                 140                 

Ala Ala Gly Pro Cys Glu Gln Ala Thr Gly Leu Gln Met Gly Val Leu 
145                 150                 155                 160 

Tyr Val Cys Leu Tyr Leu Ile Ala Leu Gly Thr Gly Gly Leu Lys Ser 
                165                 170                 175     

Ser Val Ser Gly Phe Gly Thr Asp Gln Phe Asp Glu Arg Asp Ala Arg 
            180                 185                 190         

Glu Arg Ala Ala Met Gly Leu Phe Phe Asn Arg Phe Phe Phe Phe Ile 
        195                 200                 205             

Ser Val Gly Thr Leu Leu Ala Val Thr Val Leu Val Tyr Val Gln Asp 
    210                 215                 220                 

His Val Gly Arg Ser Trp Ala Tyr Gly Ile Cys Ala Gly Ala Met Leu 
225                 230                 235                 240 

Val Ala Val Gly Val Phe Leu Ser Gly Thr Arg Arg Tyr Arg Tyr Arg 
                245                 250                 255     

Arg Ser Ser Gly Ser Pro Ile Val His Ile Leu Gln Val Leu Val Ala 
            260                 265                 270         

Ala Ala Arg Lys Arg Gly Ile Lys Gln Pro Leu Thr Ala Ser Ala Leu 
        275                 280                 285             

Tyr Glu Asp Arg Pro Asp His Ala Arg Ile Pro His Thr Ala Gln Phe 
    290                 295                 300                 

Ala Cys Leu Asp Arg Ala Ala Val Met Ala Gly Asp Asp Asp Asn Glu 
305                 310                 315                 320 

Val Gly Ser Asp Gly Arg Pro Val Pro Asn Pro Trp Lys Leu Cys Ser 
                325                 330                 335     

Val Ser Arg Val Glu Glu Val Lys Met Val Ala Arg Leu Met Pro Val 
            340                 345                 350         

Trp Ala Thr Thr Ile Leu Phe Trp Thr Ile Tyr Ala Gln Met Ile Thr 
        355                 360                 365             

Phe Ser Val Glu Gln Ala Thr Thr Met Asp Arg Arg Met Asp Gly Phe 
    370                 375                 380                 

Glu Ile Pro Ala Ala Ser Leu Thr Val Phe Phe Val Gly Ala Ile Met 
385                 390                 395                 400 

Leu Thr Leu Ala Val Tyr Asp Arg Val Phe Val Pro Leu Cys Arg Ser 
                405                 410                 415     

Leu Met Thr Gly Arg Gln Gly Leu Thr Asn Leu Glu Lys Ile Gly Ile 
            420                 425                 430         

Gly Leu Val Leu Ser Ile Val Gly Met Ala Ala Ala Ala Ser Cys Glu 
        435                 440                 445             

Lys Lys Arg Leu Ala Val Ala Ala Thr Ser Ala Ala Ala Ala Ala Gly 
    450                 455                 460                 

His Glu Pro Leu Pro Ile Ser Val Phe Met Leu Thr Pro Gln Phe Leu 
465                 470                 475                 480 

Leu Val Gly Ala Gly Glu Ala Phe Ile Tyr Thr Gly Gln Leu Asp Phe 
                485                 490                 495     

Phe Ile Thr Arg Ser Pro Lys Gly Met Lys Thr Met Ser Thr Gly Leu 
            500                 505                 510         

Phe Leu Thr Thr Leu Ser Leu Gly Phe Phe Leu Ser Ser Ala Leu Val 
        515                 520                 525             

Ser Leu Val Lys Gly Cys Thr Arg Trp Leu Gly Asp Thr Ile Asn Asn 
    530                 535                 540                 

Ser Arg Leu Asp Tyr Phe Tyr Trp Leu Leu Ala Val Leu Ser Val Val 
545                 550                 555                 560 

Asn Leu Val Ala Tyr Leu Val Cys Ala Met Trp Ala Thr Pro Pro Ala 
                565                 570                 575     

Ser Ser Gln Ala Glu Gln Pro Gln Ile Ala Met Thr Ala Asp Glu Lys 
            580                 585                 590         

Cys 
    


<210> 25
<211> 2014
<212> DNA
<213> Gossypium hirsutum

<220>
<221> misc_feature
<223> Ceres CLONE ID no. 1927461 

<220>
<221> misc_feature
<223> The DNA sequence encodes the protein given in SEQ ID NO. 26 

<400> 25
aacagcttat agttgttccc ttcatattgt agagagagga ggcttctttg cttgccacct     60

ctcttttatc tcttcaaaca cttctaaaat catggatgag aagatgagtt tgactgttgc    120

tgatgcagtg gactacaagg gtcaccctgc tgacaagtct agaactggtg gctgggtacc    180

tgctgctctc agcttaggaa ttgaaatatg tgagaggctg tcaacaatgg gaatagcagt    240

gaaccttgtg acttacttgg gtggaaccat gcaccttcca agtgctactt ctgcaaatgt    300

tgtgacagac ttcatgggga catcatttct actatgttta ctgggcggct tcctagccga    360

ttcatacttg ggcagatacc gaacgatcgc tatattctcc atggttcaga tgcttgggac    420

ttgcctgcta gcagtatcta cgaaactccc acaactacgg ccacctccgt gcaagtatac    480

cgacgtaaca caatgcgaac aagctaacag ctttcaaatg ggaattcttt atctttcatt    540

atatctaata gcattaggga ccggtggcct gaaatcaagt gtttcaggat ttggaaccga    600

ccagtttgat gagaaaaatg aaaaggagaa agcccagatg gcttattttt tcaacaggtt    660

tttctttgtc atcagcacag ggactttgat ggcagtcaca gtgcttgttt acatccaaga    720

tgaagttggt cgaagttggg gttatggaat ctgttcagct tcaatgttca ttgcactctt    780

gatattcttt tcgggaacaa aacgataccg atacaagaaa tgttcgggaa gccccattgt    840

acatatttgc caagttgttt ctgctgcatc tgggaaaaag acggctaaac tcccttctga    900

tatttccttg ttgtatgaag atctccctga ggcatcacga atccatcaca cggatcagtt    960

tcgcttcctg gacaaggcat ctgttgtgac agaagatgat tacagacaag gttccttcat   1020

gatgaaccct tggaagcttt gtccagttac aagggtagag gaggtaaaga agatgatcag   1080

gctactcccg atttgggcaa caacgattat attttggacg acatatgcac agatgatcac   1140

tttctcggtg gaacaagcaa ccaccatgga cagaaccata ggacatttcc aaattccagc   1200

tggttcactt actgtctttt ttgtgggagc catattgatc agtttagctg tttatgatcg   1260

ttttattatt ccactttgta aaaaatggag aggtgaacca gggtttacaa atctgcaact   1320

gataggcata ggtctgttcc tgtcagccat gggaatggca gctgcagcag tggtagaggt   1380

gaaacggcta tcggtggcaa agaaatacaa tgaaacaacc acactaccga taaccgtttt   1440

ctacctgatt ccgcaattct tcttggtggg tgctggagaa ggatttatat acactggtca   1500

actggatttc ttcatcactc aatccccaaa aggaatgaaa acaatgagca ctggcctctt   1560

cctcaccact ctttcactcg gtttcttctt tagtagcttg ctagtttcca ttgtgaaggc   1620

cacaaccgga agcagcgaca aacaaggatg gttgggagaa cacataaaca aaggaagatt   1680

agattgtttc tatgggctcc tagctgcatt aagcttcctt aactttggat tattccttct   1740

ttctgcagta tggttcagga agaaaacagt taaacaagct cccaaaatgg agatcgttgt   1800

taaagaatca acgatcgagg agaaatgcta gttttccata ctgaatgtta cttgaaatct   1860

tctttaataa ccaaattatt actatatata tatatatata gctagcaaag tttctgcaat   1920

atgggaactt taagctcttg tctgtaacat ggttttcctt ttggcttaag atgcaagaaa   1980

taaaacattt ccactgcaat ttgcattttc tgac                               2014


<210> 26
<211> 579
<212> PRT
<213> Gossypium hirsutum

<220>
<221> misc_feature
<223> Ceres CLONE ID no. 1927461 

<220>
<221> misc_feature
<222> (96)..(494)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1592.6 for HMM based on sequences of FIGURE 12 

<400> 26
Met Asp Glu Lys Met Ser Leu Thr Val Ala Asp Ala Val Asp Tyr Lys 
1               5                   10                  15      

Gly His Pro Ala Asp Lys Ser Arg Thr Gly Gly Trp Val Pro Ala Ala 
            20                  25                  30          

Leu Ser Leu Gly Ile Glu Ile Cys Glu Arg Leu Ser Thr Met Gly Ile 
        35                  40                  45              

Ala Val Asn Leu Val Thr Tyr Leu Gly Gly Thr Met His Leu Pro Ser 
    50                  55                  60                  

Ala Thr Ser Ala Asn Val Val Thr Asp Phe Met Gly Thr Ser Phe Leu 
65                  70                  75                  80  

Leu Cys Leu Leu Gly Gly Phe Leu Ala Asp Ser Tyr Leu Gly Arg Tyr 
                85                  90                  95      

Arg Thr Ile Ala Ile Phe Ser Met Val Gln Met Leu Gly Thr Cys Leu 
            100                 105                 110         

Leu Ala Val Ser Thr Lys Leu Pro Gln Leu Arg Pro Pro Pro Cys Lys 
        115                 120                 125             

Tyr Thr Asp Val Thr Gln Cys Glu Gln Ala Asn Ser Phe Gln Met Gly 
    130                 135                 140                 

Ile Leu Tyr Leu Ser Leu Tyr Leu Ile Ala Leu Gly Thr Gly Gly Leu 
145                 150                 155                 160 

Lys Ser Ser Val Ser Gly Phe Gly Thr Asp Gln Phe Asp Glu Lys Asn 
                165                 170                 175     

Glu Lys Glu Lys Ala Gln Met Ala Tyr Phe Phe Asn Arg Phe Phe Phe 
            180                 185                 190         

Val Ile Ser Thr Gly Thr Leu Met Ala Val Thr Val Leu Val Tyr Ile 
        195                 200                 205             

Gln Asp Glu Val Gly Arg Ser Trp Gly Tyr Gly Ile Cys Ser Ala Ser 
    210                 215                 220                 

Met Phe Ile Ala Leu Leu Ile Phe Phe Ser Gly Thr Lys Arg Tyr Arg 
225                 230                 235                 240 

Tyr Lys Lys Cys Ser Gly Ser Pro Ile Val His Ile Cys Gln Val Val 
                245                 250                 255     

Ser Ala Ala Ser Gly Lys Lys Thr Ala Lys Leu Pro Ser Asp Ile Ser 
            260                 265                 270         

Leu Leu Tyr Glu Asp Leu Pro Glu Ala Ser Arg Ile His His Thr Asp 
        275                 280                 285             

Gln Phe Arg Phe Leu Asp Lys Ala Ser Val Val Thr Glu Asp Asp Tyr 
    290                 295                 300                 

Arg Gln Gly Ser Phe Met Met Asn Pro Trp Lys Leu Cys Pro Val Thr 
305                 310                 315                 320 

Arg Val Glu Glu Val Lys Lys Met Ile Arg Leu Leu Pro Ile Trp Ala 
                325                 330                 335     

Thr Thr Ile Ile Phe Trp Thr Thr Tyr Ala Gln Met Ile Thr Phe Ser 
            340                 345                 350         

Val Glu Gln Ala Thr Thr Met Asp Arg Thr Ile Gly His Phe Gln Ile 
        355                 360                 365             

Pro Ala Gly Ser Leu Thr Val Phe Phe Val Gly Ala Ile Leu Ile Ser 
    370                 375                 380                 

Leu Ala Val Tyr Asp Arg Phe Ile Ile Pro Leu Cys Lys Lys Trp Arg 
385                 390                 395                 400 

Gly Glu Pro Gly Phe Thr Asn Leu Gln Leu Ile Gly Ile Gly Leu Phe 
                405                 410                 415     

Leu Ser Ala Met Gly Met Ala Ala Ala Ala Val Val Glu Val Lys Arg 
            420                 425                 430         

Leu Ser Val Ala Lys Lys Tyr Asn Glu Thr Thr Thr Leu Pro Ile Thr 
        435                 440                 445             

Val Phe Tyr Leu Ile Pro Gln Phe Phe Leu Val Gly Ala Gly Glu Gly 
    450                 455                 460                 

Phe Ile Tyr Thr Gly Gln Leu Asp Phe Phe Ile Thr Gln Ser Pro Lys 
465                 470                 475                 480 

Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Thr Thr Leu Ser Leu 
                485                 490                 495     

Gly Phe Phe Phe Ser Ser Leu Leu Val Ser Ile Val Lys Ala Thr Thr 
            500                 505                 510         

Gly Ser Ser Asp Lys Gln Gly Trp Leu Gly Glu His Ile Asn Lys Gly 
        515                 520                 525             

Arg Leu Asp Cys Phe Tyr Gly Leu Leu Ala Ala Leu Ser Phe Leu Asn 
    530                 535                 540                 

Phe Gly Leu Phe Leu Leu Ser Ala Val Trp Phe Arg Lys Lys Thr Val 
545                 550                 555                 560 

Lys Gln Ala Pro Lys Met Glu Ile Val Val Lys Glu Ser Thr Ile Glu 
                565                 570                 575     

Glu Lys Cys 
            


<210> 27
<211> 586
<212> PRT
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> Public GI ID no. 2760834 

<220>
<221> misc_feature
<222> (105)..(503)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1572.9 for HMM based on sequences of FIGURE 12 

<400> 27
Met Val Arg Phe Ser Ile Val Ser Gly Glu Glu Ser Lys Gly Ser Trp 
1               5                   10                  15      

Thr Val Ala Asp Ala Val Asp Tyr Lys Gly Arg Pro Ala Asp Lys Ser 
            20                  25                  30          

Lys Thr Gly Gly Trp Ile Thr Ala Ala Leu Ile Leu Gly Ile Glu Val 
        35                  40                  45              

Val Glu Arg Leu Ser Thr Met Gly Ile Ala Val Asn Leu Val Thr Tyr 
    50                  55                  60                  

Leu Met Glu Thr Met His Leu Pro Ser Ser Thr Ser Ala Asn Ile Val 
65                  70                  75                  80  

Thr Asp Phe Met Gly Thr Ser Phe Leu Leu Cys Leu Leu Gly Gly Phe 
                85                  90                  95      

Leu Ala Asp Ser Phe Leu Gly Arg Phe Lys Thr Ile Gly Ile Phe Ser 
            100                 105                 110         

Thr Ile Gln Ala Leu Gly Thr Gly Ala Leu Ala Val Ala Thr Lys Leu 
        115                 120                 125             

Pro Glu Leu Arg Pro Pro Thr Cys His His Gly Glu Ala Cys Ile Pro 
    130                 135                 140                 

Ala Thr Ala Phe Gln Met Thr Ile Leu Tyr Val Ser Leu Tyr Leu Ile 
145                 150                 155                 160 

Ala Leu Gly Thr Gly Gly Leu Lys Ser Ser Ile Ser Gly Phe Gly Ser 
                165                 170                 175     

Asp Gln Phe Asp Asp Lys Asp Pro Lys Glu Lys Ala His Met Ala Phe 
            180                 185                 190         

Phe Phe Asn Arg Phe Phe Phe Phe Ile Ser Met Gly Thr Leu Leu Ala 
        195                 200                 205             

Val Thr Val Leu Val Tyr Met Gln Asp Glu Val Gly Arg Ser Trp Ala 
    210                 215                 220                 

Tyr Gly Ile Cys Thr Val Ser Met Ala Ile Ala Ile Val Ile Phe Leu 
225                 230                 235                 240 

Cys Gly Thr Lys Arg Tyr Arg Tyr Lys Lys Ser Gln Gly Ser Pro Val 
                245                 250                 255     

Val Gln Ile Phe Gln Val Ile Ala Ala Ala Phe Arg Lys Arg Lys Met 
            260                 265                 270         

Glu Leu Pro Gln Ser Ile Val Tyr Leu Tyr Glu Asp Asn Pro Glu Gly 
        275                 280                 285             

Ile Arg Ile Glu His Thr Asp Gln Phe His Leu Leu Asp Lys Ala Ala 
    290                 295                 300                 

Ile Val Ala Glu Gly Asp Phe Glu Gln Thr Leu Asp Gly Val Ala Ile 
305                 310                 315                 320 

Pro Asn Pro Trp Lys Leu Ser Ser Val Thr Lys Val Glu Glu Val Lys 
                325                 330                 335     

Met Met Val Arg Leu Leu Pro Ile Trp Ala Thr Thr Ile Ile Phe Trp 
            340                 345                 350         

Thr Thr Tyr Ala Gln Met Ile Thr Phe Ser Val Glu Gln Ala Ser Thr 
        355                 360                 365             

Met Arg Arg Asn Ile Gly Ser Phe Lys Ile Pro Ala Gly Ser Leu Thr 
    370                 375                 380                 

Val Phe Phe Val Ala Ala Ile Leu Ile Thr Leu Ala Val Tyr Asp Arg 
385                 390                 395                 400 

Ala Ile Met Pro Phe Trp Lys Lys Trp Lys Gly Lys Pro Gly Phe Ser 
                405                 410                 415     

Ser Leu Gln Arg Ile Ala Ile Gly Leu Val Leu Ser Thr Ala Gly Met 
            420                 425                 430         

Ala Ala Ala Ala Leu Val Glu Gln Lys Arg Leu Ser Val Ala Lys Ser 
        435                 440                 445             

Ser Ser Gln Lys Thr Leu Pro Ile Ser Val Phe Leu Leu Val Pro Gln 
    450                 455                 460                 

Phe Phe Leu Val Gly Ala Gly Glu Ala Phe Ile Tyr Thr Gly Gln Leu 
465                 470                 475                 480 

Asp Phe Phe Ile Thr Gln Ser Pro Lys Gly Met Lys Thr Met Ser Thr 
                485                 490                 495     

Gly Leu Phe Leu Thr Thr Leu Ser Leu Gly Phe Phe Val Ser Ser Phe 
            500                 505                 510         

Leu Val Ser Ile Val Lys Arg Val Thr Ser Thr Ser Thr Asp Val Gly 
        515                 520                 525             

Trp Leu Ala Asp Asn Ile Asn His Gly Arg Leu Asp Tyr Phe Tyr Trp 
    530                 535                 540                 

Leu Leu Val Ile Leu Ser Gly Ile Asn Phe Val Val Tyr Ile Ile Cys 
545                 550                 555                 560 

Ala Leu Trp Phe Lys Pro Thr Lys Gly Lys Asp Ser Val Glu Lys Glu 
                565                 570                 575     

Asn Gly Lys Gly Phe Ser Val Glu Asp Cys 
            580                 585     


<210> 28
<211> 590
<212> PRT
<213> Nicotiana plumbaginifolia

<220>
<221> misc_feature
<223> Public GI ID no. 9581817 

<220>
<221> misc_feature
<222> (100)..(510)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1580.7 for HMM based on sequences of FIGURE 12 

<400> 28
Met Ala Leu Pro Glu Thr Gln Gln Asp Thr Lys Thr Leu Pro Asp Ala 
1               5                   10                  15      

Trp Asp Tyr Lys Gly Arg Pro Ala Val Arg Ser Ser Ser Gly Gly Trp 
            20                  25                  30          

Ser Ser Ala Ala Met Ile Leu Gly Ile Glu Ala Val Glu Arg Leu Thr 
        35                  40                  45              

Thr Leu Gly Ile Ala Val Asn Leu Val Thr Tyr Leu Thr Gly Thr Met 
    50                  55                  60                  

His Leu Gly Asn Ala Ser Ser Ala Asn Asn Val Thr Asn Phe Leu Gly 
65                  70                  75                  80  

Thr Ser Phe Met Leu Thr Leu Leu Gly Gly Phe Val Ala Asp Thr Phe 
                85                  90                  95      

Leu Gly Arg Tyr Leu Thr Ile Gly Ile Phe Thr Thr Ile Gln Ala Met 
            100                 105                 110         

Gly Val Thr Ile Leu Thr Ile Ser Thr Ile Ile Pro Ser Leu Arg Pro 
        115                 120                 125             

Pro Lys Cys Ser Pro Gly Ser Ser Thr Cys Ile Pro Ala Ser Ser Lys 
    130                 135                 140                 

Gln Leu Met Val Leu Tyr Ile Ala Leu Tyr Met Thr Ala Leu Gly Thr 
145                 150                 155                 160 

Gly Gly Leu Lys Ser Ser Val Ser Gly Phe Gly Ser Asp Gln Phe Asp 
                165                 170                 175     

Glu Thr Asp Lys Lys Glu Arg Gly Gln Met Ile Lys Phe Phe Asn Trp 
            180                 185                 190         

Phe Phe Phe Phe Ile Asn Val Gly Ser Leu Gly Ala Val Thr Val Leu 
        195                 200                 205             

Val Tyr Ile Gln Asp Asn Leu Gly Arg Glu Tyr Gly Tyr Gly Ile Cys 
    210                 215                 220                 

Ala Cys Ala Ile Val Ile Gly Leu Val Ile Phe Leu Ser Gly Thr Arg 
225                 230                 235                 240 

Lys Tyr Arg Phe Lys Lys Leu Val Gly Ser Pro Leu Thr Gln Ile Ala 
                245                 250                 255     

Ser Val Phe Val Ala Ala Trp Asn Lys Arg His Met Asp Leu Pro Ser 
            260                 265                 270         

Asp Ser Thr Leu Leu Tyr Asn Ile Asp Asp Ile Pro Gly Asp Gly Asn 
        275                 280                 285             

Lys Lys Ala Lys Gln Arg Leu Pro His Ser Lys Glu Phe Arg Phe Leu 
    290                 295                 300                 

Asp Lys Ala Ala Ile Lys Val Gln Asp Thr Glu Ser Ala Gly Ile Thr 
305                 310                 315                 320 

Val Val Asn Lys Trp Asn Leu Ser Thr Leu Thr Asp Val Glu Glu Val 
                325                 330                 335     

Lys Leu Val Val Arg Met Leu Pro Thr Trp Ala Thr Thr Ile Met Phe 
            340                 345                 350         

Trp Thr Val Tyr Ala Gln Met Thr Thr Phe Ser Val Ser Gln Ala Thr 
        355                 360                 365             

Thr Met Asp Arg His Ile Gly Asn Phe Glu Ile Pro Pro Ala Ser Leu 
    370                 375                 380                 

Thr Leu Phe Phe Val Gly Ser Ile Leu Leu Thr Cys Ile Phe Tyr Asp 
385                 390                 395                 400 

Arg Ala Val Val Pro Val Cys Arg Arg Val Leu Asn Asn Pro His Gly 
                405                 410                 415     

Thr Thr Pro Leu Gln Arg Ile Ala Val Gly Leu Ile Leu Ser Ile Ile 
            420                 425                 430         

Ala Met Val Ala Ala Ala Leu Thr Glu Val Lys Arg Leu Asn Val Ala 
        435                 440                 445             

His Leu His Gly Leu Thr Asn Asp Ala Asn Ala Lys Val Pro Leu Ser 
    450                 455                 460                 

Val Phe Trp Leu Val Pro Gln Phe Leu Leu Val Gly Ala Gly Glu Ala 
465                 470                 475                 480 

Phe Thr Tyr Ile Gly Gln Leu Asp Phe Phe Leu Arg Glu Cys Pro Lys 
                485                 490                 495     

Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Ser Thr Leu Ser Leu 
            500                 505                 510         

Gly Phe Phe Val Ser Ser Ile Leu Val Thr Ile Val His Lys Val Thr 
        515                 520                 525             

Val Lys Asn Pro Trp Leu Ala Asp Asn Leu Asn Gln Gly Arg Leu Tyr 
    530                 535                 540                 

Asp Phe Tyr Trp Leu Leu Ala Thr Leu Ser Val Leu Asn Leu Met Val 
545                 550                 555                 560 

Phe Leu Phe Ile Ser Arg Arg Tyr Val Tyr Lys Glu Lys Arg Leu Ala 
                565                 570                 575     

Glu Cys Gly Ile Glu Met Glu Asp Ser Glu Pro Ala Cys His 
            580                 585                 590 


<210> 29
<211> 594
<212> PRT
<213> Nicotiana plumbaginifolia

<220>
<221> misc_feature
<223> Public GI ID no. 9581819 

<220>
<221> misc_feature
<222> (100)..(513)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1470.8 for HMM based on sequences of FIGURE 12 

<400> 29
Met Ala Leu Pro Glu Thr Gln Gln Asp Ser Lys Ala Leu Pro Asp Ala 
1               5                   10                  15      

Trp Asp Tyr Lys Gly Arg Pro Ala Leu Arg Ser Ser Ser Gly Gly Trp 
            20                  25                  30          

Ala Ser Gly Ala Met Ile Leu Gly Val Glu Ala Val Glu Arg Leu Thr 
        35                  40                  45              

Thr Leu Gly Ile Ala Val Asn Leu Val Thr Tyr Leu Thr Gly Thr Met 
    50                  55                  60                  

His Leu Gly Asn Ala Thr Ala Ala Asn Asn Val Thr Asn Phe Leu Gly 
65                  70                  75                  80  

Thr Ser Phe Met Leu Thr Leu Leu Gly Gly Phe Val Ala Asp Thr Phe 
                85                  90                  95      

Leu Gly Arg Tyr Leu Thr Ile Gly Ile Phe Ala Thr Val Gln Ala Met 
            100                 105                 110         

Gly Val Thr Ile Leu Thr Ile Ser Thr Ile Ile Pro Ser Leu Arg Pro 
        115                 120                 125             

Pro Lys Cys Glu Gln Val Gly Ser Ser Ser Cys Ile Pro Ala Asn Ser 
    130                 135                 140                 

Lys Gln Leu Met Val Leu Tyr Ile Ala Leu Tyr Met Thr Ala Leu Gly 
145                 150                 155                 160 

Thr Gly Gly Leu Lys Ser Ser Val Ser Gly Phe Gly Thr Asp Gln Phe 
                165                 170                 175     

Asp Asp Gly Asp Lys Lys Glu Lys Gly Gln Met Ile Lys Phe Phe Asp 
            180                 185                 190         

Trp Phe Phe Phe Phe Ile Asn Val Gly Ser Leu Gly Ala Val Thr Val 
        195                 200                 205             

Leu Val Tyr Ile Gln Asp Asn Leu Gly Arg Glu Trp Gly Tyr Gly Ile 
    210                 215                 220                 

Cys Ala Cys Ala Ile Val Ile Gly Leu Val Val Phe Leu Ser Gly Thr 
225                 230                 235                 240 

Arg Lys Tyr Arg Phe Lys Lys Leu Val Gly Ser Pro Leu Thr Gln Ile 
                245                 250                 255     

Ala Ser Val Ile Val Ala Ala Trp Lys Lys Thr His Leu Glu Leu Pro 
            260                 265                 270         

Ser Asn Ser Ser Leu Leu Phe Glu Ile Asp Asn Ile Phe Gly Glu Gly 
        275                 280                 285             

Asn Lys Lys Ser Lys Gln Lys Leu Pro His Ser Lys Glu Tyr Arg Phe 
    290                 295                 300                 

Leu Asp Lys Ala Ala Ile Lys Glu Asp Asn Asp Leu Glu Ser Asn Gly 
305                 310                 315                 320 

Thr Lys Val Val Ile Asn Lys Trp Lys Leu Ala Thr Leu Thr Asp Val 
                325                 330                 335     

Glu Glu Val Lys Leu Leu Ile Arg Met Leu Pro Thr Trp Ala Thr Thr 
            340                 345                 350         

Ile Met Phe Trp Thr Val Tyr Ala Gln Met Thr Thr Phe Ser Val Ser 
        355                 360                 365             

Lys Ala Thr Thr Met Asp Arg His Ile Gly Thr Phe Glu Ile Pro Pro 
    370                 375                 380                 

Ala Ser Leu Thr Val Phe Phe Val Gly Ser Ile Leu Leu Thr Val Ile 
385                 390                 395                 400 

Phe Tyr Asp Arg Ile Ile Val Pro Ile Cys Arg Arg Phe Met Asn Lys 
                405                 410                 415     

Pro His Gly Leu Thr Pro Leu His Arg Ile Phe Thr Gly Leu Val Leu 
            420                 425                 430         

Ser Ile Leu Ala Met Ile Ala Ala Ala Leu Thr Glu Val Lys Arg Leu 
        435                 440                 445             

Lys Val Ala His Leu His Gly Leu Thr Asn Asp Ala Asn Ala Thr Ile 
    450                 455                 460                 

Pro Leu Thr Val Phe Trp Leu Val Pro Gln Phe Leu Leu Val Gly Ala 
465                 470                 475                 480 

Gly Glu Ala Phe Thr Tyr Ile Gly Gln Leu Asp Phe Phe Leu Arg Glu 
                485                 490                 495     

Cys Pro Lys Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Ser Thr 
            500                 505                 510         

Leu Ala Leu Gly Phe Phe Val Ser Ser Ile Leu Val Thr Ile Val His 
        515                 520                 525             

Val Val Thr Gly Thr Lys Asn Pro Trp Leu Ala Asp Asn Leu Asn Gln 
    530                 535                 540                 

Gly Arg Leu Tyr Asp Phe Tyr Trp Leu Leu Ala Ile Leu Ser Val Phe 
545                 550                 555                 560 

Asn Leu Met Phe Phe Leu Tyr Ser Ser Arg Lys Tyr Val Tyr Lys Glu 
                565                 570                 575     

Lys Arg Leu Ala Glu Met Gly Ile Glu Leu Glu Asp Asp Gly Pro Val 
            580                 585                 590         

Cys His 
        


<210> 30
<211> 577
<212> PRT
<213> Arabidopsis thaliana

<220>
<221> misc_feature
<223> Public GI ID no. 20466668 

<220>
<221> misc_feature
<222> (96)..(494)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1571.1 for HMM based on sequences of FIGURE 12 

<400> 30
Met Glu Ser Lys Gly Ser Trp Thr Val Ala Asp Ala Val Asp Tyr Lys 
1               5                   10                  15      

Gly Arg Pro Ala Asp Lys Ser Lys Thr Gly Gly Trp Ile Thr Ala Ala 
            20                  25                  30          

Leu Ile Leu Gly Ile Glu Val Val Glu Arg Leu Ser Thr Met Gly Ile 
        35                  40                  45              

Ala Val Asn Leu Val Thr Tyr Leu Met Glu Thr Met His Leu Pro Ser 
    50                  55                  60                  

Ser Thr Ser Ala Asn Ile Val Thr Asp Phe Met Gly Thr Ser Phe Leu 
65                  70                  75                  80  

Leu Cys Leu Leu Gly Gly Phe Leu Ala Asp Ser Phe Leu Gly Arg Phe 
                85                  90                  95      

Lys Thr Ile Gly Ile Phe Ser Thr Ile Gln Ala Leu Gly Thr Gly Ala 
            100                 105                 110         

Leu Ala Val Ala Thr Lys Leu Pro Glu Leu Arg Pro Pro Thr Cys His 
        115                 120                 125             

His Gly Glu Ala Cys Ile Pro Ala Thr Ala Phe Gln Met Thr Ile Leu 
    130                 135                 140                 

Tyr Val Ser Leu Tyr Leu Ile Ala Leu Gly Thr Gly Gly Leu Lys Ser 
145                 150                 155                 160 

Ser Ile Ser Gly Phe Gly Ser Asp Gln Phe Asp Asp Lys Asp Pro Lys 
                165                 170                 175     

Glu Lys Ala His Met Ala Phe Phe Phe Asn Arg Phe Phe Phe Phe Ile 
            180                 185                 190         

Ser Met Gly Thr Leu Leu Ala Val Thr Val Leu Val Tyr Met Gln Asp 
        195                 200                 205             

Glu Val Gly Arg Ser Trp Ala Tyr Gly Ile Cys Thr Val Ser Met Ala 
    210                 215                 220                 

Ile Ala Ile Val Ile Phe Leu Cys Gly Thr Lys Arg Tyr Arg Tyr Lys 
225                 230                 235                 240 

Lys Ser Gln Gly Ser Pro Val Val Gln Ile Phe Gln Val Ile Ala Ala 
                245                 250                 255     

Ala Phe Arg Lys Arg Lys Met Glu Leu Pro Gln Ser Ile Val Tyr Leu 
            260                 265                 270         

Tyr Glu Asp Asn Pro Glu Gly Ile Arg Ile Glu His Thr Asp Gln Phe 
        275                 280                 285             

His Leu Leu Asp Lys Ala Ala Ile Val Ala Glu Gly Asp Phe Glu Gln 
    290                 295                 300                 

Thr Leu Asp Gly Val Ala Ile Pro Asn Pro Trp Lys Leu Ser Ser Val 
305                 310                 315                 320 

Thr Lys Val Glu Glu Val Lys Met Met Val Arg Leu Leu Pro Ile Trp 
                325                 330                 335     

Ala Thr Thr Ile Ile Phe Trp Thr Thr Tyr Ala Gln Met Ile Thr Phe 
            340                 345                 350         

Ser Val Glu Gln Ala Ser Thr Met Arg Arg Asn Ile Gly Ser Phe Lys 
        355                 360                 365             

Ile Pro Ala Gly Ser Leu Thr Val Phe Phe Val Ala Ala Ile Leu Ile 
    370                 375                 380                 

Thr Leu Ala Val Tyr Asp Arg Ala Ile Met Pro Phe Trp Lys Lys Trp 
385                 390                 395                 400 

Lys Gly Lys Pro Gly Phe Ser Ser Leu Gln Arg Ile Ala Ile Gly Leu 
                405                 410                 415     

Val Leu Ser Thr Ala Gly Met Ala Ala Ala Ala Leu Val Glu Gln Lys 
            420                 425                 430         

Arg Leu Cys Val Ala Lys Ser Ser Ser Gln Lys Thr Leu Pro Ile Ser 
        435                 440                 445             

Val Phe Leu Leu Val Pro Gln Phe Phe Leu Val Gly Ala Gly Glu Ala 
    450                 455                 460                 

Phe Ile Tyr Thr Gly Gln Leu Asp Phe Phe Ile Thr Gln Ser Pro Lys 
465                 470                 475                 480 

Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Thr Thr Leu Ser Leu 
                485                 490                 495     

Gly Phe Phe Val Ser Ser Phe Leu Val Ser Ile Val Lys Arg Val Thr 
            500                 505                 510         

Ser Thr Ser Thr Asp Val Gly Trp Leu Ala Asp Asn Ile Asn His Gly 
        515                 520                 525             

Arg Leu Asp Tyr Phe Tyr Trp Leu Leu Val Ile Leu Ser Gly Ile Asn 
    530                 535                 540                 

Phe Val Val Tyr Ile Ile Cys Ala Leu Trp Phe Lys Pro Thr Lys Gly 
545                 550                 555                 560 

Lys Asp Ser Val Glu Lys Glu Asn Gly Lys Gly Phe Ser Val Glu Asp 
                565                 570                 575     

Cys 
    


<210> 31
<211> 590
<212> PRT
<213> Nicotiana tabacum

<220>
<221> misc_feature
<223> Public GI ID no. 28273094 

<220>
<221> misc_feature
<222> (100)..(510)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1579.1 for HMM based on sequences of FIGURE 12 

<400> 31
Met Ala Leu Pro Glu Thr Gln Gln Asp Thr Lys Thr Leu Pro Asp Ala 
1               5                   10                  15      

Trp Asp Tyr Lys Gly Arg Pro Ala Val Arg Ser Ser Ser Gly Gly Trp 
            20                  25                  30          

Ser Ser Ala Ala Met Ile Leu Gly Ile Glu Ala Val Glu Arg Leu Thr 
        35                  40                  45              

Thr Leu Gly Ile Ala Val Asn Leu Val Thr Tyr Leu Thr Gly Thr Met 
    50                  55                  60                  

His Leu Gly Asn Ala Ser Ser Ala Asn Asn Val Thr Asn Phe Leu Gly 
65                  70                  75                  80  

Thr Ser Phe Met Leu Thr Leu Leu Gly Gly Phe Val Ala Asp Thr Phe 
                85                  90                  95      

Leu Gly Arg Tyr Leu Thr Ile Gly Ile Phe Thr Thr Ile Gln Ala Met 
            100                 105                 110         

Gly Val Thr Ile Leu Thr Ile Ser Thr Ile Ile Pro Ser Leu Arg Pro 
        115                 120                 125             

Pro Lys Cys Ser Pro Gly Ser Ser Thr Cys Ile Pro Ala Ser Ser Lys 
    130                 135                 140                 

Gln Leu Met Val Leu Tyr Ile Ala Leu Tyr Met Thr Ala Leu Gly Thr 
145                 150                 155                 160 

Gly Gly Leu Lys Ser Ser Val Ser Gly Phe Gly Ser Asp Gln Phe Asp 
                165                 170                 175     

Glu Thr Asp Lys Lys Glu Arg Gly Gln Met Ile Lys Phe Phe Asn Trp 
            180                 185                 190         

Phe Phe Phe Phe Ile Asn Val Gly Ser Leu Gly Ala Val Thr Val Leu 
        195                 200                 205             

Val Tyr Ile Gln Asp Asn Leu Gly Arg Glu Tyr Gly Tyr Gly Ile Cys 
    210                 215                 220                 

Ala Cys Ala Ile Val Ile Gly Leu Val Ile Phe Leu Ser Gly Thr Arg 
225                 230                 235                 240 

Lys Tyr Arg Phe Lys Lys Leu Val Gly Ser Pro Leu Thr Gln Ile Ala 
                245                 250                 255     

Ser Val Phe Val Ala Ala Trp Asn Lys Arg His Met Asp Leu Pro Ser 
            260                 265                 270         

Asp Ser Tyr Leu Leu Tyr Asn Ile Asp Asp Ile Pro Gly Asp Gly Asn 
        275                 280                 285             

Lys Lys Ala Lys Gln Arg Leu Pro His Ser Lys Glu Phe Arg Phe Leu 
    290                 295                 300                 

Asp Lys Ala Ala Ile Lys Val Gln Asp Pro Glu Ser Ala Gly Ile Thr 
305                 310                 315                 320 

Val Val Asn Lys Trp Asn Leu Ser Thr Leu Thr Asp Val Glu Glu Val 
                325                 330                 335     

Lys Leu Val Val Arg Met Leu Pro Thr Trp Ala Thr Thr Ile Met Phe 
            340                 345                 350         

Trp Thr Val Tyr Ala Gln Met Thr Thr Phe Ser Val Ser Gln Ala Thr 
        355                 360                 365             

Thr Met Asp Arg His Ile Gly Asn Phe Glu Ile Pro Pro Ala Ser Leu 
    370                 375                 380                 

Thr Leu Phe Phe Val Gly Ser Ile Leu Leu Thr Cys Ile Phe Tyr Asp 
385                 390                 395                 400 

Arg Ala Val Val Pro Val Cys Arg Arg Val Leu Asn Asn Pro His Gly 
                405                 410                 415     

Thr Thr Pro Leu Gln Arg Ile Ala Val Gly Leu Ile Leu Ser Ile Ile 
            420                 425                 430         

Ala Met Val Ala Ala Ala Leu Thr Glu Val Lys Arg Leu Asn Val Ala 
        435                 440                 445             

His Leu His Gly Leu Thr Asn Asp Ala Asn Ala Lys Val Pro Leu Ser 
    450                 455                 460                 

Val Phe Trp Leu Val Pro Gln Phe Leu Leu Val Gly Ala Gly Glu Ala 
465                 470                 475                 480 

Phe Thr Tyr Ile Gly Gln Leu Asp Phe Phe Leu Arg Glu Cys Pro Lys 
                485                 490                 495     

Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Ser Thr Leu Ser Leu 
            500                 505                 510         

Gly Phe Phe Phe Ser Ser Ile Leu Val Thr Ile Val His Lys Val Thr 
        515                 520                 525             

Val Lys Asn Pro Trp Leu Ala Asp Asn Leu Asn Gln Gly Arg Leu Tyr 
    530                 535                 540                 

Asp Phe Tyr Trp Leu Leu Ala Thr Leu Ser Val Leu Asn Leu Met Ile 
545                 550                 555                 560 

Phe Leu Phe Ile Ser Arg Arg Tyr Val Tyr Lys Glu Lys Arg Leu Ala 
                565                 570                 575     

Glu Cys Gly Ile Glu Met Glu Asp Ser Glu Pro Ala Cys His 
            580                 585                 590 


<210> 32
<211> 590
<212> PRT
<213> Nicotiana tabacum

<220>
<221> misc_feature
<223> Public GI ID no. 28273096 

<220>
<221> misc_feature
<222> (100)..(510)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1585.9 for HMM based on sequences of FIGURE 12 

<400> 32
Met Ala Leu Pro Glu Thr Gln Gln Asp Thr Lys Thr Leu Pro Asp Ala 
1               5                   10                  15      

Trp Asp Tyr Lys Gly Arg Pro Ala Val Arg Ser Ser Ser Gly Gly Trp 
            20                  25                  30          

Ser Ser Ala Ala Met Ile Leu Gly Ile Glu Ala Val Glu Arg Leu Thr 
        35                  40                  45              

Thr Leu Gly Ile Ala Val Asn Leu Val Thr Tyr Leu Thr Gly Thr Met 
    50                  55                  60                  

His Leu Gly Asn Ala Ser Ser Ala Asn Asn Val Thr Asn Phe Leu Gly 
65                  70                  75                  80  

Thr Ser Phe Met Leu Thr Leu Leu Gly Gly Phe Val Ala Asp Thr Phe 
                85                  90                  95      

Leu Gly Arg Tyr Leu Thr Ile Gly Ile Phe Thr Thr Ile Gln Ala Met 
            100                 105                 110         

Gly Val Thr Ile Leu Thr Ile Ser Thr Ile Ile Pro Ser Leu Arg Pro 
        115                 120                 125             

Pro Lys Cys Ser Pro Gly Ser Ser Thr Cys Ile Pro Ala Ser Ser Lys 
    130                 135                 140                 

Gln Leu Met Val Leu Tyr Ile Ala Leu Tyr Met Thr Ala Leu Gly Thr 
145                 150                 155                 160 

Gly Gly Leu Lys Ser Ser Val Ser Gly Phe Gly Ser Asp Gln Phe Asp 
                165                 170                 175     

Glu Thr Asp Lys Lys Glu Arg Gly Gln Met Ile Lys Phe Phe Asn Trp 
            180                 185                 190         

Phe Phe Phe Phe Ile Asn Val Gly Ser Leu Gly Ala Val Thr Val Leu 
        195                 200                 205             

Val Tyr Ile Gln Asp Asn Leu Gly Arg Glu Tyr Gly Tyr Gly Ile Cys 
    210                 215                 220                 

Ala Cys Ala Ile Val Ile Gly Leu Val Ile Phe Leu Ser Gly Thr Arg 
225                 230                 235                 240 

Lys Tyr Arg Phe Lys Lys Leu Val Gly Ser Pro Leu Thr Gln Ile Ala 
                245                 250                 255     

Ser Val Phe Val Ala Ala Trp Asn Lys Arg His Met Glu Leu Pro Ser 
            260                 265                 270         

Asp Ser Ser Leu Leu Tyr Asn Ile Asp Asp Ile Pro Gly Asp Gly Asn 
        275                 280                 285             

Lys Lys Ala Lys Gln Arg Leu Pro His Ser Lys Glu Phe Arg Phe Leu 
    290                 295                 300                 

Asp Lys Ala Ala Ile Lys Val Gln Asp Pro Glu Ser Ala Gly Ile Thr 
305                 310                 315                 320 

Val Val Asn Lys Trp Asn Leu Ser Thr Leu Thr Asp Val Glu Glu Val 
                325                 330                 335     

Lys Leu Val Val Arg Met Leu Pro Thr Trp Ala Thr Thr Ile Met Phe 
            340                 345                 350         

Trp Thr Val Tyr Ala Gln Met Thr Thr Phe Ser Val Ser Gln Ala Thr 
        355                 360                 365             

Thr Met Asp Arg His Ile Gly Asn Phe Glu Ile Pro Pro Ala Ser Leu 
    370                 375                 380                 

Thr Leu Phe Phe Val Gly Ser Ile Leu Leu Thr Cys Ile Phe Tyr Asp 
385                 390                 395                 400 

Arg Ala Val Val Pro Val Cys Arg Arg Val Leu Asn Asn Pro His Gly 
                405                 410                 415     

Thr Ser Pro Leu Gln Arg Ile Ala Val Gly Leu Ile Leu Ser Ile Ile 
            420                 425                 430         

Ala Met Ile Ala Ala Ala Leu Thr Glu Val Lys Arg Leu Asn Val Ala 
        435                 440                 445             

His Leu His Gly Leu Thr Asn Asp Ala Asn Ala Lys Val Pro Leu Ser 
    450                 455                 460                 

Val Phe Trp Leu Val Pro Gln Phe Leu Leu Val Gly Ala Gly Glu Ala 
465                 470                 475                 480 

Phe Thr Tyr Ile Gly Gln Leu Asp Phe Phe Leu Arg Glu Cys Pro Lys 
                485                 490                 495     

Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Ser Thr Leu Ser Leu 
            500                 505                 510         

Gly Phe Phe Phe Ser Ser Ile Leu Val Thr Ile Val His Lys Val Thr 
        515                 520                 525             

Gly Lys Asn Pro Trp Leu Ala Asp Asn Leu Asn Gln Gly Arg Leu Tyr 
    530                 535                 540                 

Asp Phe Tyr Trp Leu Leu Ala Thr Leu Ser Val Leu Asn Leu Met Ile 
545                 550                 555                 560 

Phe Leu Phe Ile Ser Arg Arg Tyr Val Tyr Lys Glu Lys Arg Leu Ala 
                565                 570                 575     

Glu Cys Gly Ile Glu Met Glu Asp Ser Glu Pro Ala Cys His 
            580                 585                 590 


<210> 33
<211> 594
<212> PRT
<213> Nicotiana tabacum

<220>
<221> misc_feature
<223> Public GI ID no. 28273100 

<220>
<221> misc_feature
<222> (100)..(513)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1479.8 for HMM based on sequences of FIGURE 12 

<400> 33
Met Ala Leu Pro Glu Thr Gln Gln Asp Ser Lys Ala Leu Pro Asp Ala 
1               5                   10                  15      

Trp Asp Tyr Lys Gly Arg Pro Ala Leu Arg Ser Ser Ser Gly Gly Trp 
            20                  25                  30          

Ala Ser Gly Ala Met Ile Leu Gly Val Glu Ala Val Glu Arg Leu Thr 
        35                  40                  45              

Thr Leu Gly Ile Ala Val Asn Leu Val Thr Tyr Leu Thr Gly Thr Met 
    50                  55                  60                  

His Leu Gly Asn Ala Ser Ser Ala Asn Asn Val Thr Asn Phe Leu Gly 
65                  70                  75                  80  

Thr Ser Phe Met Leu Thr Leu Leu Gly Gly Phe Val Ala Asp Thr Phe 
                85                  90                  95      

Leu Gly Arg Tyr Leu Thr Ile Gly Ile Phe Ala Thr Val Gln Ala Met 
            100                 105                 110         

Gly Val Thr Ile Leu Thr Ile Ser Thr Ile Ile Pro Ser Leu Arg Pro 
        115                 120                 125             

Pro Lys Cys Glu Gln Val Gly Ser Ser Ser Cys Ile Pro Ala Asn Ser 
    130                 135                 140                 

Lys Gln Leu Met Val Leu Tyr Ile Ala Leu Tyr Met Thr Ala Leu Gly 
145                 150                 155                 160 

Thr Gly Gly Leu Lys Ser Ser Val Ser Gly Phe Gly Thr Asp Gln Phe 
                165                 170                 175     

Asp Asp Ser Asp Lys Lys Glu Lys Gly Gln Met Ile Lys Phe Phe Asp 
            180                 185                 190         

Trp Phe Phe Phe Phe Ile Asn Val Gly Ser Leu Gly Ala Val Thr Val 
        195                 200                 205             

Leu Val Tyr Ile Gln Asp Asn Leu Gly Arg Glu Trp Gly Tyr Gly Ile 
    210                 215                 220                 

Cys Ala Cys Ala Ile Val Ile Ala Leu Val Val Phe Leu Phe Gly Thr 
225                 230                 235                 240 

Arg Lys Tyr Arg Phe Lys Lys Leu Gly Gly Ser Pro Leu Thr Gln Ile 
                245                 250                 255     

Ala Ser Val Ile Val Ala Ala Trp Lys Lys Arg His Leu Glu Leu Pro 
            260                 265                 270         

Ser Asp Ser Ser Leu Leu Phe Glu Ile Asp Asp Ile Phe Gly Glu Gly 
        275                 280                 285             

Asn Lys Lys Ser Lys Gln Lys Leu Pro His Ser Lys Glu Tyr Arg Phe 
    290                 295                 300                 

Leu Asp Lys Ala Ala Ile Lys Glu Asp Asp Asp Leu Glu Ser Asn Gly 
305                 310                 315                 320 

Thr Asn Val Val Ile Asn Lys Trp Lys Leu Ala Thr Leu Thr Asp Val 
                325                 330                 335     

Glu Glu Val Lys Ile Leu Ile Arg Met Leu Pro Thr Trp Ala Thr Thr 
            340                 345                 350         

Ile Met Phe Trp Thr Val Tyr Ala Gln Met Thr Thr Phe Ser Val Ser 
        355                 360                 365             

Gln Ala Thr Thr Met Asp Arg His Ile Gly Thr Phe Glu Ile Pro Pro 
    370                 375                 380                 

Ala Thr Leu Thr Val Phe Phe Val Gly Ser Ile Leu Leu Thr Val Ile 
385                 390                 395                 400 

Phe Tyr Asp Arg Ile Ile Val Pro Ile Cys Arg Arg Phe Met Asn Lys 
                405                 410                 415     

Pro His Gly Leu Thr Pro Leu Gln Arg Ile Phe Thr Gly Leu Val Leu 
            420                 425                 430         

Ser Ile Leu Ala Met Ile Ala Ala Ala Leu Thr Glu Val Lys Arg Leu 
        435                 440                 445             

Lys Val Ala His Leu His Gly Leu Thr Asn Asp Ala Asn Ala Thr Ile 
    450                 455                 460                 

Pro Leu Thr Val Phe Trp Leu Val Pro Gln Phe Leu Leu Val Gly Ala 
465                 470                 475                 480 

Gly Glu Ala Phe Thr Tyr Ile Gly Gln Leu Asp Phe Phe Leu Arg Glu 
                485                 490                 495     

Cys Pro Lys Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Ser Thr 
            500                 505                 510         

Leu Ala Leu Gly Phe Phe Phe Ser Ser Ile Leu Val Thr Ile Val His 
        515                 520                 525             

Val Val Thr Gly Thr Thr Asn Pro Trp Leu Ala Asp Asn Leu Asn Gln 
    530                 535                 540                 

Gly Arg Leu Tyr Asp Phe Tyr Trp Leu Leu Ala Ile Leu Ser Val Phe 
545                 550                 555                 560 

Asn Leu Met Phe Phe Leu Tyr Ser Ser Arg Lys Tyr Val Tyr Lys Glu 
                565                 570                 575     

Lys Arg Leu Ala Glu Met Gly Ile Glu Leu Glu Asp Asp Gly Pro Val 
            580                 585                 590         

Cys His 
        


<210> 34
<211> 583
<212> PRT
<213> Alnus glutinosa

<220>
<221> misc_feature
<223> Public GI ID no. 31075168 

<220>
<221> misc_feature
<222> (97)..(503)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1513.2 for HMM based on sequences of FIGURE 12 

<400> 34
Met Glu Glu Ser Lys Lys Ser Cys Phe Ile Ser Asp Arg Val Asp Tyr 
1               5                   10                  15      

Lys Gly Asp Pro Ala Asp Arg Asn Thr Thr Gly Gly Trp Val Pro Ala 
            20                  25                  30          

Ala Leu Ile Val Gly Ile Glu Val Ser Glu Arg Leu Ser Thr Met Gly 
        35                  40                  45              

Ile Ile Val Asn Leu Val Thr Tyr Leu Lys Gly Thr Met His Gln Ser 
    50                  55                  60                  

Ser Thr Thr Ser Ser Asn Ser Thr Ser Asn Val Ala Gly Thr Ser Phe 
65                  70                  75                  80  

Leu Leu Cys Leu Leu Gly Gly Ile Leu Ala Asp Ser Phe Leu Gly Arg 
                85                  90                  95      

Tyr Trp Thr Ile Ala Ile Phe Ser Val Ile His Ala Leu Gly Thr Cys 
            100                 105                 110         

Met Leu Ala Ile Ser Ala Ala Leu Pro Glu Leu Arg Pro Ser Glu Cys 
        115                 120                 125             

Asn Leu His His Asp Phe Pro Leu Lys Cys Glu Glu Pro Ser Ser Phe 
    130                 135                 140                 

Gln Met Gly Ile Leu Tyr Thr Ala Leu Tyr Ser Met Ala Leu Gly Val 
145                 150                 155                 160 

Gly Gly Ile Lys Ser Ser Val Ser Gly Phe Gly Thr Glu Gln Phe Asp 
                165                 170                 175     

Gln Asn Asp Glu Lys Glu Met Ser Glu Met Ala His Phe Phe Asn Arg 
            180                 185                 190         

Phe Tyr Leu Ile Ile Ser Phe Gly Thr Leu Leu Ala Val Thr Val Leu 
        195                 200                 205             

Val Tyr Val Gln Asp Gln Val Gly Arg Ser Trp Gly Tyr Gly Ile Cys 
    210                 215                 220                 

Ser Ala Ser Met Phe Leu Ser Ile Val Ala Phe Leu Val Gly Thr Lys 
225                 230                 235                 240 

Arg Tyr Arg Tyr Arg Glu Cys Thr Gly Ser Pro Ile Ile Gln Ile Leu 
                245                 250                 255     

Gln Val Ile Val Ala Ala Ile Arg Lys Arg Lys Ala Glu Leu Pro Thr 
            260                 265                 270         

Asp Leu Ser Phe Leu Tyr Asp Lys Ala Ser Asp Lys Glu Ser Thr Ile 
        275                 280                 285             

Ser His Thr Asp Lys Tyr Ser Cys Leu Asp Lys Gly Ala Ile Ile Thr 
    290                 295                 300                 

Gly Met Asp Arg Gly Ala Asp Gly Leu Thr Ile Ser Asn Pro Trp Gly 
305                 310                 315                 320 

Leu Cys Thr Val Thr Glu Val Glu Glu Val Lys Met Leu Ile Arg Ile 
                325                 330                 335     

Leu Pro Ile Met Ala Ser Thr Ile Ile Phe Trp Thr Ile Arg Ala Gln 
            340                 345                 350         

Leu Leu Ser Phe Ser Val Gln Gln Ala Ala Thr Met Glu Arg Thr Ile 
        355                 360                 365             

Glu Asn Phe Pro Ile Pro Pro Ala Ser Phe Asn Gly Phe Phe Val Gly 
    370                 375                 380                 

Ser Thr Ile Ile Thr Leu Ile Met Tyr Asp Arg Leu Leu Pro Phe Leu 
385                 390                 395                 400 

Arg Gln Ser Ser Asn Gly Val Gln Gly Phe Thr Asn Leu Gln Lys Ile 
                405                 410                 415     

Gly Ile Ala Ile Phe Leu Ser Ile Leu Gly Met Ala Ala Ala Ser Phe 
            420                 425                 430         

Ala Glu Met Lys Arg Leu Glu Val Val Arg Ala Asn Arg Gly Ser Thr 
        435                 440                 445             

Ser Thr Ser Ser Thr Leu Pro Ile Thr Ala Phe Tyr Leu Leu Pro Gln 
    450                 455                 460                 

Phe Val Leu Val Gly Ile Gly Asp Gly Phe Met Tyr Thr Gly Gln Leu 
465                 470                 475                 480 

Asp Phe Phe Ile Thr Glu Ser Pro Lys Gly Met Lys Ala Ile Ser Thr 
                485                 490                 495     

Gly Leu Phe Leu Thr Thr Asn Ala Leu Gly Phe Phe Gly Ser Ser Ile 
            500                 505                 510         

Leu Val Thr Ile Ile Thr Lys Val Thr Gly Glu Glu Val Gly His Gly 
        515                 520                 525             

Trp Leu Leu Ser Arg Ile Asn Asp Ser Arg Leu Asp Phe Phe Tyr Ala 
    530                 535                 540                 

Leu Leu Ala Val Leu Gly Phe Ile Asn Leu Val Phe Tyr Leu Val Leu 
545                 550                 555                 560 

Ala Ser Trp Tyr Lys Pro Ser Pro Val Glu Asp Ala His Gln Lys Ile 
                565                 570                 575     

Asn Gly Lys Glu Glu Lys Val 
            580             


<210> 35
<211> 596
<212> PRT
<213> Prunus persica

<220>
<221> misc_feature
<223> Public GI ID no. 33411520 

<220>
<221> misc_feature
<222> (102)..(514)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1604.6 for HMM based on sequences of FIGURE 12 

<400> 35
Met Ser Asn Cys Thr Leu Pro Glu Thr Gln Glu Lys Leu Thr Leu Pro 
1               5                   10                  15      

Asp Ala Trp Asp Phe Lys Gly Arg Pro Ala Glu Arg Ser Lys Thr Gly 
            20                  25                  30          

Gly Trp Thr Ala Ala Ala Met Ile Leu Gly Gly Glu Ala Cys Glu Arg 
        35                  40                  45              

Leu Thr Thr Leu Gly Ile Ala Val Asn Leu Val Thr Tyr Leu Thr Gly 
    50                  55                  60                  

Thr Met His Leu Gly Asn Ala Thr Ser Ala Asn Thr Val Thr Asn Phe 
65                  70                  75                  80  

Leu Gly Thr Ser Phe Met Leu Cys Leu Leu Gly Gly Phe Val Ala Asp 
                85                  90                  95      

Thr Phe Leu Gly Arg Tyr Leu Thr Ile Ala Ile Phe Ala Thr Phe Gln 
            100                 105                 110         

Ala Met Gly Val Thr Ile Leu Thr Ile Ser Thr Thr Ile Pro Ser Leu 
        115                 120                 125             

Arg Pro Pro Lys Cys Thr Ser Asp Thr Ser Thr Pro Cys Ile Pro Ala 
    130                 135                 140                 

Ser Gly Lys Gln Leu Met Val Leu Tyr Ile Ala Leu Tyr Leu Thr Ala 
145                 150                 155                 160 

Leu Gly Thr Gly Gly Leu Lys Ser Ser Val Ser Gly Phe Gly Ser Asp 
                165                 170                 175     

Gln Phe Asp Glu Ser Asp Lys Gln Glu Arg Arg Gln Met Thr Asn Phe 
            180                 185                 190         

Phe Asn Trp Phe Phe Phe Phe Ile Ser Ile Gly Ser Leu Ala Ala Val 
        195                 200                 205             

Thr Val Leu Val Tyr Ile Gln Asp Asn Leu Gly Arg Gln Trp Gly Tyr 
    210                 215                 220                 

Gly Ile Cys Val Cys Ala Ile Val Leu Gly Leu Ile Val Phe Leu Ser 
225                 230                 235                 240 

Gly Thr Arg Arg Tyr Arg Phe Lys Lys Leu Val Gly Ser Pro Leu Thr 
                245                 250                 255     

Gln Ile Ser Gly Val Cys Val Ala Ala Trp Arg Lys Arg Asn Met Glu 
            260                 265                 270         

Leu Pro Ser Asp Met Ser Phe Leu Tyr Asn Val Asp Asp Ile Asp Asp 
        275                 280                 285             

Gly Leu Lys Lys Lys Lys Lys Gln Lys Leu Pro His Ser Lys Gln Phe 
    290                 295                 300                 

Arg Phe Leu Asp Lys Ala Ala Ile Lys Glu Pro Lys Thr Thr Ser Gly 
305                 310                 315                 320 

Thr Ala Met Ile Ile Asn Lys Trp Ser Leu Ser Thr Leu Thr Asp Val 
                325                 330                 335     

Glu Glu Val Lys Leu Ile Ile Arg Met Leu Pro Ile Trp Ala Thr Thr 
            340                 345                 350         

Ile Met Phe Trp Thr Val Tyr Ala Gln Met Thr Thr Phe Ser Val Ser 
        355                 360                 365             

Gln Ala Thr Ser Met Asp Arg His Ile Gly Lys Ser Phe Gln Ile Pro 
    370                 375                 380                 

Pro Ala Ser Leu Thr Ala Phe Phe Val Gly Ser Ile Leu Leu Thr Val 
385                 390                 395                 400 

Pro Val Tyr Asp Arg Leu Ile Val Pro Met Ala Arg Lys Ala Leu Glu 
                405                 410                 415     

Asn Pro Gln Gly Leu Thr Pro Leu Gln Arg Met Gly Val Gly Leu Val 
            420                 425                 430         

Phe Ser Ile Phe Ala Met Val Ala Ala Ala Leu Thr Glu Val Lys Arg 
        435                 440                 445             

Leu Asn Ile Ala Arg Ser His Gly Leu Thr Asp Asn Pro Thr Ala Glu 
    450                 455                 460                 

Ile Pro Leu Ser Val Phe Trp Leu Val Pro Gln Phe Phe Phe Val Gly 
465                 470                 475                 480 

Ser Gly Glu Ala Phe Thr Tyr Ile Gly Gln Leu Asp Phe Phe Leu Arg 
                485                 490                 495     

Glu Cys Pro Lys Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Ser 
            500                 505                 510         

Thr Leu Ser Leu Gly Phe Phe Phe Ser Ser Leu Leu Val Thr Ile Val 
        515                 520                 525             

His Lys Thr Thr Gly His Asn Lys Pro Trp Leu Ala Asp Asn Leu Asn 
    530                 535                 540                 

Gln Gly Lys Leu Tyr Asp Phe Tyr Trp Leu Leu Ala Leu Leu Ser Ala 
545                 550                 555                 560 

Leu Asn Leu Val Ile Tyr Leu Phe Cys Ala Asn Trp Tyr Val Tyr Lys 
                565                 570                 575     

Asp Lys Arg Leu Ala Glu Glu Gly Ile Glu Leu Glu Glu Pro Glu Ile 
            580                 585                 590         

Cys Ala His Ala 
        595     


<210> 36
<211> 594
<212> PRT
<213> Prunus persica

<220>
<221> misc_feature
<223> Public GI ID no. 48675345 

<220>
<221> misc_feature
<222> (102)..(514)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1597 for HMM based on sequences of FIGURE 12 

<400> 36
Met Ser Asn Cys Thr Leu Pro Glu Thr Gln Glu Lys Leu Thr Leu Pro 
1               5                   10                  15      

Asp Ala Trp Asp Phe Lys Gly Arg Pro Ala Glu Arg Ser Lys Thr Gly 
            20                  25                  30          

Gly Trp Thr Ala Ala Ala Met Ile Leu Gly Gly Glu Ala Cys Glu Arg 
        35                  40                  45              

Leu Thr Thr Leu Gly Ile Ala Val Asn Leu Val Thr Tyr Leu Thr Gly 
    50                  55                  60                  

Thr Met His Leu Gly Asn Ala Thr Ser Ala Asn Thr Val Thr Asn Phe 
65                  70                  75                  80  

Leu Gly Thr Ser Phe Met Leu Cys Leu Leu Gly Gly Phe Val Ala Asp 
                85                  90                  95      

Thr Phe Leu Gly Arg Tyr Leu Thr Ile Ala Ile Phe Ala Thr Phe Gln 
            100                 105                 110         

Ala Met Gly Val Thr Ile Leu Thr Ile Ser Thr Thr Ile Pro Ser Leu 
        115                 120                 125             

Arg Pro Pro Lys Cys Thr Ser Asp Thr Ser Thr Pro Cys Ile Pro Ala 
    130                 135                 140                 

Ser Gly Lys Gln Leu Met Val Leu Tyr Ile Ala Leu Tyr Leu Thr Ala 
145                 150                 155                 160 

Leu Gly Thr Gly Gly Leu Lys Ser Ser Val Ser Gly Phe Gly Ser Asp 
                165                 170                 175     

Gln Phe Asp Glu Ser Asp Lys Gln Glu Arg Arg Gln Met Thr Asn Phe 
            180                 185                 190         

Phe Asn Trp Phe Phe Phe Phe Ile Ser Ile Gly Ser Leu Ala Ala Val 
        195                 200                 205             

Thr Val Leu Val Tyr Ile Gln Asp Asn Leu Gly Arg Gln Trp Gly Tyr 
    210                 215                 220                 

Gly Ile Cys Val Cys Ala Ile Val Leu Gly Leu Ile Val Phe Leu Ser 
225                 230                 235                 240 

Gly Thr Arg Arg Tyr Arg Phe Lys Lys Leu Val Gly Ser Pro Leu Thr 
                245                 250                 255     

Gln Ile Ser Gly Val Cys Val Ala Ala Trp Arg Lys Arg Asn Met Glu 
            260                 265                 270         

Leu Pro Ser Asp Met Ser Phe Leu Tyr Asn Val Asp Asp Ile Asp Asp 
        275                 280                 285             

Gly Leu Lys Lys Lys Lys Lys Gln Lys Leu Pro His Ser Lys Gln Phe 
    290                 295                 300                 

Arg Phe Leu Asp Lys Ala Ala Ile Lys Glu Pro Lys Thr Thr Ser Gly 
305                 310                 315                 320 

Thr Ala Met Ile Ile Asn Lys Trp Ser Leu Ser Thr Leu Thr Asp Val 
                325                 330                 335     

Glu Glu Val Lys Leu Ile Ile Arg Met Leu Pro Ile Trp Ala Thr Thr 
            340                 345                 350         

Ile Met Phe Trp Thr Val Tyr Ala Gln Met Thr Thr Phe Ser Val Ser 
        355                 360                 365             

Gln Ala Thr Ser Met Asp Arg His Ile Gly Lys Ser Phe Gln Ile Pro 
    370                 375                 380                 

Pro Ala Ser Leu Thr Ala Phe Phe Val Gly Ser Ile Leu Leu Thr Val 
385                 390                 395                 400 

Pro Val Tyr Asp Arg Leu Ile Val Pro Met Ala Arg Lys Ala Leu Glu 
                405                 410                 415     

Asn Pro Gln Gly Leu Thr Pro Leu Gln Arg Met Gly Val Gly Leu Val 
            420                 425                 430         

Phe Ser Ile Phe Ala Met Val Ala Ala Ala Leu Thr Glu Val Lys Arg 
        435                 440                 445             

Leu Asn Ile Ala Arg Ser His Gly Leu Thr Asp Asn Pro Thr Ala Glu 
    450                 455                 460                 

Ile Pro Leu Ser Val Phe Trp Leu Val Pro Gln Phe Phe Phe Val Gly 
465                 470                 475                 480 

Ser Gly Glu Ala Phe Thr Tyr Ile Gly Gln Leu Asp Phe Phe Leu Arg 
                485                 490                 495     

Glu Cys Pro Lys Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Ser 
            500                 505                 510         

Thr Leu Ser Leu Gly Phe Phe Phe Ser Ser Leu Leu Val Thr Ile Val 
        515                 520                 525             

His Lys Thr Thr Gly His Asn Lys Pro Trp Leu Ala Asp Asn Leu Asn 
    530                 535                 540                 

Gln Gly Lys Leu Tyr Asp Phe Tyr Trp Leu Leu Ala Leu Leu Ser Ala 
545                 550                 555                 560 

Leu Asn Leu Val Ile Tyr Leu Phe Cys Ala Asn Trp Tyr Val Tyr Lys 
                565                 570                 575     

Asp Lys Arg Leu Ala Glu Glu Gly Ile Glu Leu Glu Glu Pro Glu Ile 
            580                 585                 590         

Cys Ala 
        


<210> 37
<211> 587
<212> PRT
<213> Oryza sativa subsp. japonica

<220>
<221> misc_feature
<223> Public GI ID no. 57899246 

<220>
<221> misc_feature
<222> (110)..(508)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1496.9 for HMM based on sequences of FIGURE 12 

<400> 37
Met Val Arg Glu Val Glu Lys Lys Thr Ser Ala Trp Ser Gly Gly Gly 
1               5                   10                  15      

Gly Gly Gly Gly Asn Met Val Arg Asp Ala Val Asp Tyr Arg Gly Cys 
            20                  25                  30          

Pro Ala Asp Arg Ser Ala Thr Gly Gly Trp Val Ala Ala Ala Leu Val 
        35                  40                  45              

Leu Gly Ile Glu Leu Cys Glu Arg Leu Ser Thr Met Gly Ile Ala Val 
    50                  55                  60                  

Asn Leu Val Thr Tyr Leu Thr Gly Thr Met His Leu Pro Ser Ala Ala 
65                  70                  75                  80  

Ala Ala Asn Val Val Thr Asp Phe Met Gly Thr Ser Phe Leu Leu Cys 
                85                  90                  95      

Leu Leu Gly Gly Phe Leu Ala Asp Ser Phe Leu Gly Arg Tyr Leu Thr 
            100                 105                 110         

Ile Ala Val Phe Ala Leu Val Gln Ser Ile Gly Thr Ala Leu Leu Ala 
        115                 120                 125             

Ala Ser Thr Leu Val Thr His Leu Arg Pro Pro Pro Gly Glu Gln Pro 
    130                 135                 140                 

Thr Pro Val Gln Met Ala Val Leu Tyr Ala Cys Leu Tyr Leu Ile Ala 
145                 150                 155                 160 

Leu Gly Thr Gly Gly Leu Lys Ser Ser Val Ser Gly Phe Gly Thr Asp 
                165                 170                 175     

Gln Phe Asp Asp Arg Asp Gly Arg Glu Arg Ala Ala Met Gly Leu Phe 
            180                 185                 190         

Phe Asn Arg Phe Phe Phe Phe Ile Ser Leu Gly Thr Leu Leu Ala Val 
        195                 200                 205             

Thr Val Leu Val Tyr Val Gln Asp His Val Gly Arg Ser Trp Ala Tyr 
    210                 215                 220                 

Gly Ile Cys Ser Ala Ala Met Leu Ala Ala Ile Ala Val Phe Leu Ser 
225                 230                 235                 240 

Gly Thr Arg Arg Tyr Arg Tyr Lys Arg Ser Ser Gly Ser Pro Ile Val 
                245                 250                 255     

His Ile Leu Gln Val Leu Val Ala Ala Ala Arg Lys Arg Gly Val Val 
            260                 265                 270         

Lys Arg Pro Pro Thr Ala Ala Glu Leu Tyr Glu Asp Asp Arg Pro Glu 
        275                 280                 285             

His Ala Arg Ile Ala His Thr Ala Gln Phe Pro Cys Leu Asp Met Ala 
    290                 295                 300                 

Ala Val Val Ala Gly Glu Glu Asp Asn Glu Val Ala Gly Pro Gly Gly 
305                 310                 315                 320 

Pro Ala Ala Pro Asn Pro Trp Lys Leu Cys Ser Val Ser Arg Val Glu 
                325                 330                 335     

Glu Val Lys Met Val Ala Arg Leu Met Pro Val Trp Ala Thr Thr Ile 
            340                 345                 350         

Leu Phe Trp Thr Ile Tyr Ala Gln Met Ile Thr Phe Ser Val Glu Gln 
        355                 360                 365             

Ala Thr Thr Met Asp Arg Arg Val Gly Ala Gly Phe Glu Ile Pro Ala 
    370                 375                 380                 

Ala Ser Leu Thr Val Phe Phe Val Gly Ala Ile Met Leu Thr Leu Ala 
385                 390                 395                 400 

Val Tyr Asp Arg Val Phe Ile Pro Leu Cys Arg Val Leu Thr Gly Lys 
                405                 410                 415     

Gln Gly Phe Thr Asn Leu Glu Lys Ile Gly Ile Gly Leu Ala Leu Ser 
            420                 425                 430         

Ile Leu Gly Met Ala Ala Ala Ala Leu Cys Glu Lys Lys Arg Leu Ala 
        435                 440                 445             

Val Ala Val Ala Ala Thr Thr Gly Asn Ser Thr Pro Ile Ser Val Phe 
    450                 455                 460                 

Leu Leu Thr Pro Gln Phe Leu Leu Val Gly Ala Gly Glu Ala Phe Ile 
465                 470                 475                 480 

Tyr Thr Gly Gln Leu Asp Phe Phe Ile Thr Arg Ser Pro Lys Gly Met 
                485                 490                 495     

Lys Thr Met Ser Thr Gly Leu Phe Leu Thr Thr Leu Ser Leu Gly Phe 
            500                 505                 510         

Phe Phe Ser Ser Val Leu Val Ser Leu Val Lys Gly Ala Thr Thr Trp 
        515                 520                 525             

Leu Gly Asp Thr Ile Asp Arg Ser Arg Leu Asp Tyr Phe Tyr Trp Leu 
    530                 535                 540                 

Leu Ala Val Leu Ser Val Leu Asn Leu Ala Ala Tyr Leu Val Cys Ala 
545                 550                 555                 560 

Lys Trp Ala Ala Thr Ala Ala Ala Thr Ser Arg Glu Gln Gln Gln Gln 
                565                 570                 575     

His Thr Ala Val Ala Asp Ala Asp Glu Lys Cys 
            580                 585         


<210> 38
<211> 566
<212> PRT
<213> Oryza sativa subsp. japonica

<220>
<221> misc_feature
<223> Public GI ID no. 115437568 

<220>
<221> misc_feature
<222> (89)..(487)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> The sequence is a functional homolog of the NRT 1.4 
      Variant at SEQ ID NO. 17 

<220>
<221> misc_feature
<223> Bit score of 1482.5 for HMM based on sequences of FIGURE 12 

<400> 38
Met Val Arg Asp Ala Val Asp Tyr Arg Gly Cys Pro Ala Asp Arg Ser 
1               5                   10                  15      

Ala Thr Gly Gly Trp Val Ala Ala Ala Leu Val Leu Gly Ile Glu Leu 
            20                  25                  30          

Cys Glu Arg Leu Ser Thr Met Gly Ile Ala Val Asn Leu Val Thr Tyr 
        35                  40                  45              

Leu Thr Gly Thr Met His Leu Pro Ser Ala Ala Ala Ala Asn Val Val 
    50                  55                  60                  

Thr Asp Phe Met Gly Thr Ser Phe Leu Leu Cys Leu Leu Gly Gly Phe 
65                  70                  75                  80  

Leu Ala Asp Ser Phe Leu Gly Arg Tyr Leu Thr Ile Ala Val Phe Ala 
                85                  90                  95      

Leu Val Gln Ser Ile Gly Thr Ala Leu Leu Ala Ala Ser Thr Leu Val 
            100                 105                 110         

Thr His Leu Arg Pro Pro Pro Gly Glu Gln Pro Thr Pro Val Gln Met 
        115                 120                 125             

Ala Val Leu Tyr Ala Cys Leu Tyr Leu Ile Ala Leu Gly Thr Gly Gly 
    130                 135                 140                 

Leu Lys Ser Ser Val Ser Gly Phe Gly Thr Asp Gln Phe Asp Asp Arg 
145                 150                 155                 160 

Asp Gly Arg Glu Arg Ala Ala Met Gly Leu Phe Phe Asn Arg Phe Phe 
                165                 170                 175     

Phe Phe Ile Ser Leu Gly Thr Leu Leu Ala Val Thr Val Leu Val Tyr 
            180                 185                 190         

Val Gln Asp His Val Gly Arg Ser Trp Ala Tyr Gly Ile Cys Ser Ala 
        195                 200                 205             

Ala Met Leu Ala Ala Ile Ala Val Phe Leu Ser Gly Thr Arg Arg Tyr 
    210                 215                 220                 

Arg Tyr Lys Arg Ser Ser Gly Ser Pro Ile Val His Ile Leu Gln Val 
225                 230                 235                 240 

Leu Val Ala Ala Ala Arg Lys Arg Gly Val Val Lys Arg Pro Pro Thr 
                245                 250                 255     

Ala Ala Glu Leu Tyr Glu Asp Asp Arg Pro Glu His Ala Arg Ile Ala 
            260                 265                 270         

His Thr Ala Gln Phe Pro Cys Leu Asp Met Ala Ala Val Val Ala Gly 
        275                 280                 285             

Glu Glu Asp Asn Glu Val Ala Gly Pro Gly Gly Pro Ala Ala Pro Asn 
    290                 295                 300                 

Pro Trp Lys Leu Cys Ser Val Ser Arg Val Glu Glu Val Lys Met Val 
305                 310                 315                 320 

Ala Arg Leu Met Pro Val Trp Ala Thr Thr Ile Leu Phe Trp Thr Ile 
                325                 330                 335     

Tyr Ala Gln Met Ile Thr Phe Ser Val Glu Gln Ala Thr Thr Met Asp 
            340                 345                 350         

Arg Arg Val Gly Ala Gly Phe Glu Ile Pro Ala Ala Ser Leu Thr Val 
        355                 360                 365             

Phe Phe Val Gly Ala Ile Met Leu Thr Leu Ala Val Tyr Asp Arg Val 
    370                 375                 380                 

Phe Ile Pro Leu Cys Arg Val Leu Thr Gly Lys Gln Gly Phe Thr Asn 
385                 390                 395                 400 

Leu Glu Lys Ile Gly Ile Gly Leu Ala Leu Ser Ile Leu Gly Met Ala 
                405                 410                 415     

Ala Ala Ala Leu Cys Glu Lys Lys Arg Leu Ala Val Ala Val Ala Ala 
            420                 425                 430         

Thr Thr Gly Asn Ser Thr Pro Ile Ser Val Phe Leu Leu Thr Pro Gln 
        435                 440                 445             

Phe Leu Leu Val Gly Ala Gly Glu Ala Phe Ile Tyr Thr Gly Gln Leu 
    450                 455                 460                 

Asp Phe Phe Ile Thr Arg Ser Pro Lys Gly Met Lys Thr Met Ser Thr 
465                 470                 475                 480 

Gly Leu Phe Leu Thr Thr Leu Ser Leu Gly Phe Phe Phe Ser Ser Val 
                485                 490                 495     

Leu Val Ser Leu Val Lys Gly Ala Thr Thr Trp Leu Gly Asp Thr Ile 
            500                 505                 510         

Asp Arg Ser Arg Leu Asp Tyr Phe Tyr Trp Leu Leu Ala Val Leu Ser 
        515                 520                 525             

Val Leu Asn Leu Ala Ala Tyr Leu Val Cys Ala Lys Trp Ala Ala Thr 
    530                 535                 540                 

Ala Ala Ala Thr Ser Arg Glu Gln Gln Gln Gln His Thr Ala Val Ala 
545                 550                 555                 560 

Asp Ala Asp Glu Lys Cys 
                565     


<210> 39
<211> 1764
<212> DNA
<213> Populus balsamifera subsp. trichocarpa

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 8785142 

<220>
<221> misc_feature
<223> Encodes the peptide sequence at SEQ ID NO. 40 

<400> 39
atggttttag caacaagcca cagtgggaaa gatggtgcgc acgctgatcc tgcagtagac  60    

tttcgaggaa atcctgtgga caagtccaga actggaggat ggcttgctgc ggggctcatt  120   

ttggggaccg aactttcaga gagaatatgc gtcatgggca tatcaatgaa tttagtgaca  180   

tacctagtag gagagctgca tatttcatca gcaaaatctg caaccatagt caccaatttc  240   

atgggtactc tcaaccttct cggcctccta ggaggtttct tggcagatgc taagcttggt  300   

cggtacttgg cagttgctat cttcgcatcc ataactgctt tggggctcag tctattaacc  360   

atggccacaa ccattcctac catgagaccc ccacagtgcg atgactacag gaaacagcat  420   

tatcagtgta tcgaggccag tggaaatcaa ctggctctgc tatatgcagc actttatacc  480   

acagcacttg gtggtggtgg aattaagtct aatgtctcag gttttggatc tgatcagttc  540   

gatcaaacag atcccaagga ggagaaggcg atgcatttct tcttcaatag gttctatttt  600   

tgcattagca tcgggtcatt gtttgcggtt atagtgcttg tatacataca agataacgtg  660   

ggaagagggt ggggatatgg aatttcagcg gggacgatgg tgatcgcagt agcagtattg  720   

ctctgtggga caccgtggta ccgtttcaag aagcctcgag gtagcccatt aactaccata  780   

tggagggtat cactcttggc atggaagaag aggaggcacc cttatccttc ccatcccagc  840   

cttctgaatg attatgacag tgccacagtt ccccacacag agaaattcaa gtatcttgac  900   

aaggctgcaa tcatggatcc cacctccagc gcacaaaaaa atgacccttg gatagtatcg  960   

acagttacgg aagttgaaga agtgaagatg gtactaaagc tcatcccaat ttggtctaca  1020  

tgtatcctct tttggacagt ctactctcaa atgactactt tcaccattga acaagcgacc  1080  

ttcatgaaca gaaaagttgg ctccttggtt attccttcag gttcatactc cacctttctc  1140  

tttatcacca ttctcctctt tacatcccta aatgaaagac tcttcattcc cctggctcga  1200  

aagctcaccc gcaatgcaca aggactgaca agccttcaaa ggattggaat tggactcttt  1260  

ctttcaatac tagctatggt ggctgctgct atcattgaga aagaaagaag ggaaactgtt  1320  

attcaacaaa atacaatgat aagtgccttt tggctagtcc ctcaatattt cctagtgggt  1380  

gccggggagg ctttcgttta tgttggacaa cttgagtttt tcatcagaga ggcaccagaa  1440  

aggatgaaat caatgagcac tgggcttttc cttagcagca tctcaatggg tttctttgtt  1500  

agcagtttgt tggtgtcttt agttgacaaa gctaccaaga aaatctggct caggagcaac  1560  

ttgaataaag ggaggttaaa taacttctat tggatgcttg cagtgctagg actgttgaac  1620  

ctattcattt tcctagtttt tgcaagggag catcagtaca aagtccagca gtatatcaag  1680  

cccaagaact ctgaagagaa agagcttaaa actgcaaatg atttgactgt tttagagatg  1740  

gtgaaggagg aggaaatgat ttaa                                         1764  


<210> 40
<211> 587
<212> PRT
<213> Populus balsamifera subsp. trichocarpa

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 8785142 

<220>
<221> misc_feature
<222> (1)..(587)
<223> Bit score of 1401.8 for hmm based on sequences of FIGURE 11. 

<220>
<221> misc_feature
<222> (102)..(494)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Functional Homolog Of SEQ ID NO. 2 

<400> 40
Met Val Leu Ala Thr Ser His Ser Gly Lys Asp Gly Ala His Ala Asp 
1               5                   10                  15      

Pro Ala Val Asp Phe Arg Gly Asn Pro Val Asp Lys Ser Arg Thr Gly 
            20                  25                  30          

Gly Trp Leu Ala Ala Gly Leu Ile Leu Gly Thr Glu Leu Ser Glu Arg 
        35                  40                  45              

Ile Cys Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly 
    50                  55                  60                  

Glu Leu His Ile Ser Ser Ala Lys Ser Ala Thr Ile Val Thr Asn Phe 
65                  70                  75                  80  

Met Gly Thr Leu Asn Leu Leu Gly Leu Leu Gly Gly Phe Leu Ala Asp 
                85                  90                  95      

Ala Lys Leu Gly Arg Tyr Leu Ala Val Ala Ile Phe Ala Ser Ile Thr 
            100                 105                 110         

Ala Leu Gly Leu Ser Leu Leu Thr Met Ala Thr Thr Ile Pro Thr Met 
        115                 120                 125             

Arg Pro Pro Gln Cys Asp Asp Tyr Arg Lys Gln His Tyr Gln Cys Ile 
    130                 135                 140                 

Glu Ala Ser Gly Asn Gln Leu Ala Leu Leu Tyr Ala Ala Leu Tyr Thr 
145                 150                 155                 160 

Thr Ala Leu Gly Gly Gly Gly Ile Lys Ser Asn Val Ser Gly Phe Gly 
                165                 170                 175     

Ser Asp Gln Phe Asp Gln Thr Asp Pro Lys Glu Glu Lys Ala Met His 
            180                 185                 190         

Phe Phe Phe Asn Arg Phe Tyr Phe Cys Ile Ser Ile Gly Ser Leu Phe 
        195                 200                 205             

Ala Val Ile Val Leu Val Tyr Ile Gln Asp Asn Val Gly Arg Gly Trp 
    210                 215                 220                 

Gly Tyr Gly Ile Ser Ala Gly Thr Met Val Ile Ala Val Ala Val Leu 
225                 230                 235                 240 

Leu Cys Gly Thr Pro Trp Tyr Arg Phe Lys Lys Pro Arg Gly Ser Pro 
                245                 250                 255     

Leu Thr Thr Ile Trp Arg Val Ser Leu Leu Ala Trp Lys Lys Arg Arg 
            260                 265                 270         

His Pro Tyr Pro Ser His Pro Ser Leu Leu Asn Asp Tyr Asp Ser Ala 
        275                 280                 285             

Thr Val Pro His Thr Glu Lys Phe Lys Tyr Leu Asp Lys Ala Ala Ile 
    290                 295                 300                 

Met Asp Pro Thr Ser Ser Ala Gln Lys Asn Asp Pro Trp Ile Val Ser 
305                 310                 315                 320 

Thr Val Thr Glu Val Glu Glu Val Lys Met Val Leu Lys Leu Ile Pro 
                325                 330                 335     

Ile Trp Ser Thr Cys Ile Leu Phe Trp Thr Val Tyr Ser Gln Met Thr 
            340                 345                 350         

Thr Phe Thr Ile Glu Gln Ala Thr Phe Met Asn Arg Lys Val Gly Ser 
        355                 360                 365             

Leu Val Ile Pro Ser Gly Ser Tyr Ser Thr Phe Leu Phe Ile Thr Ile 
    370                 375                 380                 

Leu Leu Phe Thr Ser Leu Asn Glu Arg Leu Phe Ile Pro Leu Ala Arg 
385                 390                 395                 400 

Lys Leu Thr Arg Asn Ala Gln Gly Leu Thr Ser Leu Gln Arg Ile Gly 
                405                 410                 415     

Ile Gly Leu Phe Leu Ser Ile Leu Ala Met Val Ala Ala Ala Ile Ile 
            420                 425                 430         

Glu Lys Glu Arg Arg Glu Thr Val Ile Gln Gln Asn Thr Met Ile Ser 
        435                 440                 445             

Ala Phe Trp Leu Val Pro Gln Tyr Phe Leu Val Gly Ala Gly Glu Ala 
    450                 455                 460                 

Phe Val Tyr Val Gly Gln Leu Glu Phe Phe Ile Arg Glu Ala Pro Glu 
465                 470                 475                 480 

Arg Met Lys Ser Met Ser Thr Gly Leu Phe Leu Ser Ser Ile Ser Met 
                485                 490                 495     

Gly Phe Phe Val Ser Ser Leu Leu Val Ser Leu Val Asp Lys Ala Thr 
            500                 505                 510         

Lys Lys Ile Trp Leu Arg Ser Asn Leu Asn Lys Gly Arg Leu Asn Asn 
        515                 520                 525             

Phe Tyr Trp Met Leu Ala Val Leu Gly Leu Leu Asn Leu Phe Ile Phe 
    530                 535                 540                 

Leu Val Phe Ala Arg Glu His Gln Tyr Lys Val Gln Gln Tyr Ile Lys 
545                 550                 555                 560 

Pro Lys Asn Ser Glu Glu Lys Glu Leu Lys Thr Ala Asn Asp Leu Thr 
                565                 570                 575     

Val Leu Glu Met Val Lys Glu Glu Glu Met Ile 
            580                 585         


<210> 41
<211> 1779
<212> DNA
<213> Solanum lycopersicum

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 8457650 

<220>
<221> misc_feature
<223> Encodes the peptide sequence at SEQ ID NO. 42 

<400> 41
atggttttag ttcataacca tgctgaaaaa gatggttcaa atgatggagg tctagtcgat  60    

tttcgtggaa atccagttga taaatcgcgt acaggaggat ggcttggtgc agggcttatt  120   

ttaggtacag agttatcaga aaggatatgt gttatgggga tatcgatgaa tttggtgact  180   

tatttggttg gagatttaca tcttccatct tctgattctg ccaacattgt tactaatttc  240   

atgggaacac ttaatcttct tgcacttctt ggtggtttct tggctgatgc taaactcgga  300   

cgttatgcca ctattgcaat ctttggttgc atagccgctg tgggagtaac actgttgaca  360   

cttgcaacat cgatcccaag tatgaagcct cccgtgtgtg actcaagatc gaaaggtcat  420   

tgcattgaag ccagcgggca gcagcttgct ctcctctacg cagcattata caccatatcg  480   

cttggttgtg gggggataaa gtctagtgtg tctggttttg gatcagacca gtttgattca  540   

tcgaatccta aagagaataa ggccatgata tacttcttta acaggttcta cttctgcatt  600   

agccttggat ctttgtttgc tgtgactgtg ctagtgtata tacaagacaa tgttggaagg  660   

gggtggggtt atggtatatc agctggaaca atggtactag ctgtggctgt tttgctttgc  720   

ggaacatcgt tgtatagatt taagaagcca gagggaagtc ctttgactat tatatggcga  780   

gttttgatat tggcttggcg aaagaggaaa atttctcacc cttctgatcc tggattcttg  840   

aatgaatatc ataacgctaa agtaccacat acgaaaatgc taagatgttt agacaaggca  900   

gcgattattg atgattatgc agttgcagat gaaaacagaa tcagttcatg gatagtatca  960   

acggttagtc aagtcgaaga agtgaaaatg gttcttaagt tgattccgat atggtctaca  1020  

tgtatcctct tttggacggt ctactctcaa atgaacacgt tcagtatcga gcaagctacc  1080  

ttcatgaatc gaaatgttgg caaatttggc atcccagcag gctcattttc cttctttctc  1140  

ttcatttcta tactcttgtt tacttcccta aacgaaagag tcaccgtacc aatagctaga  1200  

aggatcacgg gcaatagaca aggactcaca agccttcaaa gagttggaat tggacttata  1260  

ctatctattg ttggtatggt agctgcagct gttgtagaga aacaacgaag ggaaaacgcg  1320  

atacaccatg attataacat aagtgcattt tggttagtcc ctcagttttt catcgttggt  1380  

gctggtgaag cttttgccta cgttggacaa ctcgagttct tcatcagaga agcaccagaa  1440  

ggaatgaaat caatgagcac ggggctattc ctcagcacgc tttcaatggg attttttatg  1500  

agtagcttgt tagtgtcgat tgtacataag gtaacaaacg gaagctggct taaaaacaac  1560  

ttgaacaacg gtaaactaga ctacttctac tggatgttag cagtactcgg agtactcaat  1620  

tacttcgtgt tccttgtttt ctcaacgaga catcagtaca agacacaaca tcttagtact  1680  

actcttgaag actcggaaga agagctcaag aattggaacg atacgagcat tgacaacaca  1740  

caaaagaatc ctaatgatgc agagaaggaa caagtttaa                         1779  


<210> 42
<211> 592
<212> PRT
<213> Solanum lycopersicum

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 8457650 

<220>
<221> misc_feature
<222> (1)..(592)
<223> Bit score of 1260.8 for hmm based on sequences of FIGURE 11. 

<220>
<221> misc_feature
<222> (102)..(494)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Functional Homolog Of SEQ ID NO. 2 

<400> 42
Met Val Leu Val His Asn His Ala Glu Lys Asp Gly Ser Asn Asp Gly 
1               5                   10                  15      

Gly Leu Val Asp Phe Arg Gly Asn Pro Val Asp Lys Ser Arg Thr Gly 
            20                  25                  30          

Gly Trp Leu Gly Ala Gly Leu Ile Leu Gly Thr Glu Leu Ser Glu Arg 
        35                  40                  45              

Ile Cys Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly 
    50                  55                  60                  

Asp Leu His Leu Pro Ser Ser Asp Ser Ala Asn Ile Val Thr Asn Phe 
65                  70                  75                  80  

Met Gly Thr Leu Asn Leu Leu Ala Leu Leu Gly Gly Phe Leu Ala Asp 
                85                  90                  95      

Ala Lys Leu Gly Arg Tyr Ala Thr Ile Ala Ile Phe Gly Cys Ile Ala 
            100                 105                 110         

Ala Val Gly Val Thr Leu Leu Thr Leu Ala Thr Ser Ile Pro Ser Met 
        115                 120                 125             

Lys Pro Pro Val Cys Asp Ser Arg Ser Lys Gly His Cys Ile Glu Ala 
    130                 135                 140                 

Ser Gly Gln Gln Leu Ala Leu Leu Tyr Ala Ala Leu Tyr Thr Ile Ser 
145                 150                 155                 160 

Leu Gly Cys Gly Gly Ile Lys Ser Ser Val Ser Gly Phe Gly Ser Asp 
                165                 170                 175     

Gln Phe Asp Ser Ser Asn Pro Lys Glu Asn Lys Ala Met Ile Tyr Phe 
            180                 185                 190         

Phe Asn Arg Phe Tyr Phe Cys Ile Ser Leu Gly Ser Leu Phe Ala Val 
        195                 200                 205             

Thr Val Leu Val Tyr Ile Gln Asp Asn Val Gly Arg Gly Trp Gly Tyr 
    210                 215                 220                 

Gly Ile Ser Ala Gly Thr Met Val Leu Ala Val Ala Val Leu Leu Cys 
225                 230                 235                 240 

Gly Thr Ser Leu Tyr Arg Phe Lys Lys Pro Glu Gly Ser Pro Leu Thr 
                245                 250                 255     

Ile Ile Trp Arg Val Leu Ile Leu Ala Trp Arg Lys Arg Lys Ile Ser 
            260                 265                 270         

His Pro Ser Asp Pro Gly Phe Leu Asn Glu Tyr His Asn Ala Lys Val 
        275                 280                 285             

Pro His Thr Lys Met Leu Arg Cys Leu Asp Lys Ala Ala Ile Ile Asp 
    290                 295                 300                 

Asp Tyr Ala Val Ala Asp Glu Asn Arg Ile Ser Ser Trp Ile Val Ser 
305                 310                 315                 320 

Thr Val Ser Gln Val Glu Glu Val Lys Met Val Leu Lys Leu Ile Pro 
                325                 330                 335     

Ile Trp Ser Thr Cys Ile Leu Phe Trp Thr Val Tyr Ser Gln Met Asn 
            340                 345                 350         

Thr Phe Ser Ile Glu Gln Ala Thr Phe Met Asn Arg Asn Val Gly Lys 
        355                 360                 365             

Phe Gly Ile Pro Ala Gly Ser Phe Ser Phe Phe Leu Phe Ile Ser Ile 
    370                 375                 380                 

Leu Leu Phe Thr Ser Leu Asn Glu Arg Val Thr Val Pro Ile Ala Arg 
385                 390                 395                 400 

Arg Ile Thr Gly Asn Arg Gln Gly Leu Thr Ser Leu Gln Arg Val Gly 
                405                 410                 415     

Ile Gly Leu Ile Leu Ser Ile Val Gly Met Val Ala Ala Ala Val Val 
            420                 425                 430         

Glu Lys Gln Arg Arg Glu Asn Ala Ile His His Asp Tyr Asn Ile Ser 
        435                 440                 445             

Ala Phe Trp Leu Val Pro Gln Phe Phe Ile Val Gly Ala Gly Glu Ala 
    450                 455                 460                 

Phe Ala Tyr Val Gly Gln Leu Glu Phe Phe Ile Arg Glu Ala Pro Glu 
465                 470                 475                 480 

Gly Met Lys Ser Met Ser Thr Gly Leu Phe Leu Ser Thr Leu Ser Met 
                485                 490                 495     

Gly Phe Phe Met Ser Ser Leu Leu Val Ser Ile Val His Lys Val Thr 
            500                 505                 510         

Asn Gly Ser Trp Leu Lys Asn Asn Leu Asn Asn Gly Lys Leu Asp Tyr 
        515                 520                 525             

Phe Tyr Trp Met Leu Ala Val Leu Gly Val Leu Asn Tyr Phe Val Phe 
    530                 535                 540                 

Leu Val Phe Ser Thr Arg His Gln Tyr Lys Thr Gln His Leu Ser Thr 
545                 550                 555                 560 

Thr Leu Glu Asp Ser Glu Glu Glu Leu Lys Asn Trp Asn Asp Thr Ser 
                565                 570                 575     

Ile Asp Asn Thr Gln Lys Asn Pro Asn Asp Ala Glu Lys Glu Gln Val 
            580                 585                 590         


<210> 43
<211> 1809
<212> DNA
<213> Solanum lycopersicum

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 8457852 

<220>
<221> misc_feature
<223> Encodes the peptide sequence at SEQ ID NO. 44 

<400> 43
atggttttgg ttgataacca tgctggaaaa gatggtgcag aagatggtaa tatggttgat  60    

tttcgaggaa atccggtgga taagtctagg acagggggat ggctagctgc aggacttatc  120   

ctaggaactg agctatcaga aagggtatgt gttatgggga tttcgatgaa tttagtgacg  180   

tacttagttg gagatttaca tcttccatcc tccaaatctg ccaacattgt caccaatttc  240   

atggggacac ttaatcttct tggtcttcta ggtggtttct tggcagatgc taaactcgga  300   

cgttatctga ctgttggaat ctttgcttca attgctgctg tgtgttttaa tagacgagag  360   

cttgtgtcta accagggggt tacgcttttg acattggcga catccattcc aggcatgaag  420   

ccgcctgaat gtaacccaag aaaaagtggt cactgcattg aagccagtgg ccagcagctt  480   

gctcttctct atacggcgct ttacatccta gctcttggtg gtggtggaat taagtcaaat  540   

gtctccgggt ttggttcaga ccaatttgac tcatcagatc ctaaggagaa caagtccatg  600   

atatacttct tcaacagatt ctatttctgc ataagccttg gttctctgtt tgcagtgact  660   

gtgctggtgt acttacaaga caatgtagga agaggatggg gatatgggat atcagcaggc  720   

acaatggtcc tcggggtcgc tgtattgatt ggtggaacga cgttgtatcg attcaagaag  780   

cctcaaggaa gtcctttgac tatcatatgg agggttctgc ttttagcttg gaggaagaga  840   

aagcttagtt acccttctga tactggcttc ttgaatgaat atcacaatgc caaagtccca  900   

catacacata tgttgaggtg tcttgacaag gcagccattc ttgatgactc tgcagctgca  960   

aatgagaata gcaagaatcg ttggatagtt tcaacagtta cagaagtcga agaagtgaaa  1020  

atggtgctca aattgattcc catatggtcc acatgcatac ttttttggac agtatactct  1080  

cagatgaata ccttcaccat tgaacaagct accttcatga accggaatgt tggaaacttt  1140  

gctgtccctg caggttcctt atccgtgttt ctctttatta gcatacttct gtttacttcc  1200  

ataaacgaaa gggtcacagt tcgtattgcc agaaaaatca ctcacaacag ccaaggaatc  1260  

acaagccttc agagagttgg aattggacta ctactctcta ttgttggtat ggtagcttca  1320  

gctctggtag aaaaacgacg aagggaacat gccatccatc ataacttcaa gataagcgcg  1380  

ttttggttag tgcctcaatt cttcattgta ggtgctgggg aagcttttgc ctatgtagga  1440  

cagctagagt ttttcatcag ggaggcacca gaagggatga aatctatgag cacaggccta  1500  

tttctcagca cactctcgat gggatatttc gtgagtagtt tgctagtatt cgttgtacag  1560  

aaagcaacaa aaggaagatg gcttaaaagc aatttaaaca aaggaaaact ggatttattc  1620  

tactggttgc tagcagttct cggagtaatt aatttcttga ttttcattgc attttcaatg  1680  

aaacaccaat acaaggtgca gaaacttagc agtattgagg attctgcaga agagctcggg  1740  

agttggaagg atttgaccct cgacaacaag gaaaagaaac tcgaagcaga cgagaaggtg  1800  

gaagcttaa                                                          1809  


<210> 44
<211> 602
<212> PRT
<213> Solanum lycopersicum

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 8457852 

<220>
<221> misc_feature
<222> (1)..(602)
<223> Bit score of 1235.7 for hmm based on sequences of FIGURE 11. 

<220>
<221> misc_feature
<222> (102)..(505)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Functional Homolog Of SEQ ID NO. 2 

<400> 44
Met Val Leu Val Asp Asn His Ala Gly Lys Asp Gly Ala Glu Asp Gly 
1               5                   10                  15      

Asn Met Val Asp Phe Arg Gly Asn Pro Val Asp Lys Ser Arg Thr Gly 
            20                  25                  30          

Gly Trp Leu Ala Ala Gly Leu Ile Leu Gly Thr Glu Leu Ser Glu Arg 
        35                  40                  45              

Val Cys Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly 
    50                  55                  60                  

Asp Leu His Leu Pro Ser Ser Lys Ser Ala Asn Ile Val Thr Asn Phe 
65                  70                  75                  80  

Met Gly Thr Leu Asn Leu Leu Gly Leu Leu Gly Gly Phe Leu Ala Asp 
                85                  90                  95      

Ala Lys Leu Gly Arg Tyr Leu Thr Val Gly Ile Phe Ala Ser Ile Ala 
            100                 105                 110         

Ala Val Cys Phe Asn Arg Arg Glu Leu Val Ser Asn Gln Gly Val Thr 
        115                 120                 125             

Leu Leu Thr Leu Ala Thr Ser Ile Pro Gly Met Lys Pro Pro Glu Cys 
    130                 135                 140                 

Asn Pro Arg Lys Ser Gly His Cys Ile Glu Ala Ser Gly Gln Gln Leu 
145                 150                 155                 160 

Ala Leu Leu Tyr Thr Ala Leu Tyr Ile Leu Ala Leu Gly Gly Gly Gly 
                165                 170                 175     

Ile Lys Ser Asn Val Ser Gly Phe Gly Ser Asp Gln Phe Asp Ser Ser 
            180                 185                 190         

Asp Pro Lys Glu Asn Lys Ser Met Ile Tyr Phe Phe Asn Arg Phe Tyr 
        195                 200                 205             

Phe Cys Ile Ser Leu Gly Ser Leu Phe Ala Val Thr Val Leu Val Tyr 
    210                 215                 220                 

Leu Gln Asp Asn Val Gly Arg Gly Trp Gly Tyr Gly Ile Ser Ala Gly 
225                 230                 235                 240 

Thr Met Val Leu Gly Val Ala Val Leu Ile Gly Gly Thr Thr Leu Tyr 
                245                 250                 255     

Arg Phe Lys Lys Pro Gln Gly Ser Pro Leu Thr Ile Ile Trp Arg Val 
            260                 265                 270         

Leu Leu Leu Ala Trp Arg Lys Arg Lys Leu Ser Tyr Pro Ser Asp Thr 
        275                 280                 285             

Gly Phe Leu Asn Glu Tyr His Asn Ala Lys Val Pro His Thr His Met 
    290                 295                 300                 

Leu Arg Cys Leu Asp Lys Ala Ala Ile Leu Asp Asp Ser Ala Ala Ala 
305                 310                 315                 320 

Asn Glu Asn Ser Lys Asn Arg Trp Ile Val Ser Thr Val Thr Glu Val 
                325                 330                 335     

Glu Glu Val Lys Met Val Leu Lys Leu Ile Pro Ile Trp Ser Thr Cys 
            340                 345                 350         

Ile Leu Phe Trp Thr Val Tyr Ser Gln Met Asn Thr Phe Thr Ile Glu 
        355                 360                 365             

Gln Ala Thr Phe Met Asn Arg Asn Val Gly Asn Phe Ala Val Pro Ala 
    370                 375                 380                 

Gly Ser Leu Ser Val Phe Leu Phe Ile Ser Ile Leu Leu Phe Thr Ser 
385                 390                 395                 400 

Ile Asn Glu Arg Val Thr Val Arg Ile Ala Arg Lys Ile Thr His Asn 
                405                 410                 415     

Ser Gln Gly Ile Thr Ser Leu Gln Arg Val Gly Ile Gly Leu Leu Leu 
            420                 425                 430         

Ser Ile Val Gly Met Val Ala Ser Ala Leu Val Glu Lys Arg Arg Arg 
        435                 440                 445             

Glu His Ala Ile His His Asn Phe Lys Ile Ser Ala Phe Trp Leu Val 
    450                 455                 460                 

Pro Gln Phe Phe Ile Val Gly Ala Gly Glu Ala Phe Ala Tyr Val Gly 
465                 470                 475                 480 

Gln Leu Glu Phe Phe Ile Arg Glu Ala Pro Glu Gly Met Lys Ser Met 
                485                 490                 495     

Ser Thr Gly Leu Phe Leu Ser Thr Leu Ser Met Gly Tyr Phe Val Ser 
            500                 505                 510         

Ser Leu Leu Val Phe Val Val Gln Lys Ala Thr Lys Gly Arg Trp Leu 
        515                 520                 525             

Lys Ser Asn Leu Asn Lys Gly Lys Leu Asp Leu Phe Tyr Trp Leu Leu 
    530                 535                 540                 

Ala Val Leu Gly Val Ile Asn Phe Leu Ile Phe Ile Ala Phe Ser Met 
545                 550                 555                 560 

Lys His Gln Tyr Lys Val Gln Lys Leu Ser Ser Ile Glu Asp Ser Ala 
                565                 570                 575     

Glu Glu Leu Gly Ser Trp Lys Asp Leu Thr Leu Asp Asn Lys Glu Lys 
            580                 585                 590         

Lys Leu Glu Ala Asp Glu Lys Val Glu Ala 
        595                 600         


<210> 45
<211> 1794
<212> DNA
<213> Sorghum bicolor

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 8679906 

<220>
<221> misc_feature
<223> Encodes the peptide sequence at SEQ ID NO. 46 

<400> 45
atggtttccg ccggggttca tggtggcggc ggcgacgggc aggaggcggt ggacttccga  60    

ggcaacccgg tggacaagtc gaggaccgga gggtggctgg gcgccgggct gatcctgggc  120   

acggagctgg cggagcgcgt gtgcgtcatg ggcatctcca tgaacctggt cacgtacctc  180   

gtcggcgagc ttcacctctc caactccaag tccgccaacg tcgtcaccaa cttcatgggc  240   

acgctcaacc tcctcgccct cgtcggcggc ttcctcgccg acgccaagct cggccgctac  300   

ctcaccatcg ccatctccgc caccgtcgcc gccaccggcg tgagcttgct gacggtggac  360   

acgacggtgc cgagcatgcg gccgccggcg tgcgcgaacg cccgcgggcc gcgcgcgcac  420   

caggactgcg tgccggcgac cggcgggcag ctggcgctgc tgtacgcggc gctgtacacg  480   

gtcgcggcgg gcgccggcgg gctcaaggcg aacgtgtccg ggttcgggtc ggaccagttc  540   

gacgcggggg acccgcggga ggagcgcgcc atggtgttct tcttcaaccg cttctacttc  600   

tgcgtcagcc tgggctccct gttcgcggtc accgtgctgg tgtacgtgca ggacaacgtg  660   

ggcaggtgct ggggctacgg cgtctccgcc gtcgccatgc tgctcgccgt cgccgtgctc  720   

gtcgccggca cgcccaggta ccggtaccgc cgcccgcagg gaagcccgct cacggtcatc  780   

ggccgggtgc tcgccaccgc gtggaggaag aggaggttga cgctaccggc ggacgccgcc  840   

gagctccacg ggttcgccgc cgccaaggtc gcccatacgg acaggctcag gtgccttgac  900   

aaggcggcga tcgtggaggc cgacctgtcc gcgccggcgg ggaagcagca gcagcaggcg  960   

agcgcgccgg cgtcgacggt gacggaggtg gaggaggtga agatggtggt gaagctgctg  1020  

cccatctggt ccacgtgcat cctcttctgg acggtctact cccagatgac caccttctcg  1080  

gtggagcagg ccacgcgcat ggaccgccac ctccgcccgg gctcctcctt cgccgtcccg  1140  

gcgggctccc tctccgtgtt cctcttcatc tccatcctgc tcttcacctc cctcaacgag  1200  

cgcctcctgg tgccgctcgc cgcgcgcctc acgggccgcc cgcaggggct cacctcgctg  1260  

cagcgggtcg ggacggggct cgcgctctcc gtcgccgcca tggccgtctc ggcgctcgtc  1320  

gagaagaagc ggcgcgacgc gtccaatggc cccggccacg tcgccatcag cgccttctgg  1380  

ctcgtcccgc agttcttcct cgtcggcgcc ggcgaggcgt tcgcgtacgt ggggcagctg  1440  

gagttcttca tccgggaggc gcccgagcgg atgaagtcca tgagcaccgg cctgttcctc  1500  

gtcacgctct ccatgggctt cttcctcagc agcttcctcg tcttcgccgt cgacgccgtc  1560  

accggcggcg cgtggatccg gaacaacctc gaccgcggaa ggcttgacct cttctactgg  1620  

atgctcgccg tgctcggggt cgcaaacttc gccgtcttca tcgtcttcgc caggcggcac  1680  

cagtacaagg ccagcaacct gccggcggcg gtggcgcccg acggcgccgc caggaagaag  1740  

gagacggacg acttcgtcgc cgtggcggag gcagtcgaag gaatggacgt gtag        1794  


<210> 46
<211> 597
<212> PRT
<213> Sorghum bicolor

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 8679906 

<220>
<221> misc_feature
<222> (1)..(597)
<223> Bit score of 1428.1 for hmm based on sequences of FIGURE 11. 

<220>
<221> misc_feature
<222> (100)..(503)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Functional Homolog Of SEQ ID NO. 2 

<400> 46
Met Val Ser Ala Gly Val His Gly Gly Gly Gly Asp Gly Gln Glu Ala 
1               5                   10                  15      

Val Asp Phe Arg Gly Asn Pro Val Asp Lys Ser Arg Thr Gly Gly Trp 
            20                  25                  30          

Leu Gly Ala Gly Leu Ile Leu Gly Thr Glu Leu Ala Glu Arg Val Cys 
        35                  40                  45              

Val Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly Glu Leu 
    50                  55                  60                  

His Leu Ser Asn Ser Lys Ser Ala Asn Val Val Thr Asn Phe Met Gly 
65                  70                  75                  80  

Thr Leu Asn Leu Leu Ala Leu Val Gly Gly Phe Leu Ala Asp Ala Lys 
                85                  90                  95      

Leu Gly Arg Tyr Leu Thr Ile Ala Ile Ser Ala Thr Val Ala Ala Thr 
            100                 105                 110         

Gly Val Ser Leu Leu Thr Val Asp Thr Thr Val Pro Ser Met Arg Pro 
        115                 120                 125             

Pro Ala Cys Ala Asn Ala Arg Gly Pro Arg Ala His Gln Asp Cys Val 
    130                 135                 140                 

Pro Ala Thr Gly Gly Gln Leu Ala Leu Leu Tyr Ala Ala Leu Tyr Thr 
145                 150                 155                 160 

Val Ala Ala Gly Ala Gly Gly Leu Lys Ala Asn Val Ser Gly Phe Gly 
                165                 170                 175     

Ser Asp Gln Phe Asp Ala Gly Asp Pro Arg Glu Glu Arg Ala Met Val 
            180                 185                 190         

Phe Phe Phe Asn Arg Phe Tyr Phe Cys Val Ser Leu Gly Ser Leu Phe 
        195                 200                 205             

Ala Val Thr Val Leu Val Tyr Val Gln Asp Asn Val Gly Arg Cys Trp 
    210                 215                 220                 

Gly Tyr Gly Val Ser Ala Val Ala Met Leu Leu Ala Val Ala Val Leu 
225                 230                 235                 240 

Val Ala Gly Thr Pro Arg Tyr Arg Tyr Arg Arg Pro Gln Gly Ser Pro 
                245                 250                 255     

Leu Thr Val Ile Gly Arg Val Leu Ala Thr Ala Trp Arg Lys Arg Arg 
            260                 265                 270         

Leu Thr Leu Pro Ala Asp Ala Ala Glu Leu His Gly Phe Ala Ala Ala 
        275                 280                 285             

Lys Val Ala His Thr Asp Arg Leu Arg Cys Leu Asp Lys Ala Ala Ile 
    290                 295                 300                 

Val Glu Ala Asp Leu Ser Ala Pro Ala Gly Lys Gln Gln Gln Gln Ala 
305                 310                 315                 320 

Ser Ala Pro Ala Ser Thr Val Thr Glu Val Glu Glu Val Lys Met Val 
                325                 330                 335     

Val Lys Leu Leu Pro Ile Trp Ser Thr Cys Ile Leu Phe Trp Thr Val 
            340                 345                 350         

Tyr Ser Gln Met Thr Thr Phe Ser Val Glu Gln Ala Thr Arg Met Asp 
        355                 360                 365             

Arg His Leu Arg Pro Gly Ser Ser Phe Ala Val Pro Ala Gly Ser Leu 
    370                 375                 380                 

Ser Val Phe Leu Phe Ile Ser Ile Leu Leu Phe Thr Ser Leu Asn Glu 
385                 390                 395                 400 

Arg Leu Leu Val Pro Leu Ala Ala Arg Leu Thr Gly Arg Pro Gln Gly 
                405                 410                 415     

Leu Thr Ser Leu Gln Arg Val Gly Thr Gly Leu Ala Leu Ser Val Ala 
            420                 425                 430         

Ala Met Ala Val Ser Ala Leu Val Glu Lys Lys Arg Arg Asp Ala Ser 
        435                 440                 445             

Asn Gly Pro Gly His Val Ala Ile Ser Ala Phe Trp Leu Val Pro Gln 
    450                 455                 460                 

Phe Phe Leu Val Gly Ala Gly Glu Ala Phe Ala Tyr Val Gly Gln Leu 
465                 470                 475                 480 

Glu Phe Phe Ile Arg Glu Ala Pro Glu Arg Met Lys Ser Met Ser Thr 
                485                 490                 495     

Gly Leu Phe Leu Val Thr Leu Ser Met Gly Phe Phe Leu Ser Ser Phe 
            500                 505                 510         

Leu Val Phe Ala Val Asp Ala Val Thr Gly Gly Ala Trp Ile Arg Asn 
        515                 520                 525             

Asn Leu Asp Arg Gly Arg Leu Asp Leu Phe Tyr Trp Met Leu Ala Val 
    530                 535                 540                 

Leu Gly Val Ala Asn Phe Ala Val Phe Ile Val Phe Ala Arg Arg His 
545                 550                 555                 560 

Gln Tyr Lys Ala Ser Asn Leu Pro Ala Ala Val Ala Pro Asp Gly Ala 
                565                 570                 575     

Ala Arg Lys Lys Glu Thr Asp Asp Phe Val Ala Val Ala Glu Ala Val 
            580                 585                 590         

Glu Gly Met Asp Val 
        595         


<210> 47
<211> 683
<212> PRT
<213> Oryza sativa subsp. japonica

<220>
<221> misc_feature
<223> Public GI ID no. 125582644 

<220>
<221> misc_feature
<222> (1)..(683)
<223> Bit score of 1420.5 for hmm based on sequences of FIGURE 11. 

<220>
<221> misc_feature
<222> (182)..(582)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Functional Homolog Of SEQ ID NO. 2 

<400> 47
Met Glu Asn Glu Trp Met His Ala Arg Leu Glu Lys Leu Arg Arg Ser 
1               5                   10                  15      

Gly Arg Gln Cys Ile Ser Ala Ala Glu Glu Asn Gly Asn Ile Asn Asn 
            20                  25                  30          

Ala Cys Leu Cys Cys Ile Cys Ala Cys Ser Arg Met His Val Ala Ala 
        35                  40                  45              

Gly Gly Gly His Gly Gly Gly Ala Asp Asp Gly Met Val Val Asp Tyr 
    50                  55                  60                  

Arg Gly Asn Pro Val Asp Lys Ser Lys Thr Gly Gly Trp Leu Gly Ala 
65                  70                  75                  80  

Gly Leu Ile Leu Gly Thr Glu Leu Ala Glu Arg Val Cys Val Met Gly 
                85                  90                  95      

Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly Asp Leu His Leu Ser 
            100                 105                 110         

Asn Ala Lys Ser Ala Asn Ile Val Thr Asn Phe Met Gly Thr Leu Asn 
        115                 120                 125             

Leu Leu Ala Leu Val Gly Gly Phe Leu Ala Asp Ala Lys Leu Gly Arg 
    130                 135                 140                 

Tyr Leu Thr Ile Ala Ile Ser Ala Thr Ile Ala Ala Thr Val Arg Ser 
145                 150                 155                 160 

Arg Ser Arg Thr His Arg Thr Thr His Leu Arg Ser Ser Ser Arg Gly 
                165                 170                 175     

Ala Gly Ile Ser Leu Arg Ser Ala Ala Met Gln Gly Val Ser Leu Leu 
            180                 185                 190         

Thr Val Asp Thr Met Val Pro Gly Met Arg Pro Pro Pro Cys Ala Asp 
        195                 200                 205             

Ala Arg Gly Ala Gly Ala His Leu Arg Cys Glu Pro Ala Arg Gly Gly 
    210                 215                 220                 

Gln Leu Ala Met Leu Tyr Ala Ala Leu Tyr Thr Val Ala Ala Gly Ala 
225                 230                 235                 240 

Gly Gly Leu Lys Ala Asn Val Ser Gly Phe Gly Ser Asp Gln Phe Asp 
                245                 250                 255     

Gly Gly Asp Pro Arg Glu Glu Arg Ala Met Val Phe Phe Phe Asn Arg 
            260                 265                 270         

Phe Tyr Phe Cys Ile Ser Leu Gly Ser Leu Phe Ala Val Thr Val Leu 
        275                 280                 285             

Val Tyr Val Gln Asp Asn Val Gly Arg Gly Trp Gly Tyr Gly Val Ser 
    290                 295                 300                 

Ala Val Ala Met Val Leu Ala Val Ala Val Leu Val Ala Gly Thr Pro 
305                 310                 315                 320 

Lys Tyr Arg Tyr Arg Arg Pro Glu Gly Ser Pro Leu Thr Val Ile Gly 
                325                 330                 335     

Arg Val Leu Ala Thr Ala Trp Arg Lys Arg Arg Leu Pro Leu Pro Ala 
            340                 345                 350         

Asp Ala Gly Glu Leu His Gly Tyr His Thr Ser Lys Val Ala Tyr Thr 
        355                 360                 365             

Asp Arg Leu Arg Cys Leu Asp Arg Ala Ala Ile Met Glu Ala Asp Leu 
    370                 375                 380                 

Ala Ala Ser Pro Ala Lys Thr Asn Gln Thr Ser Ala Ala Pro Ala Ala 
385                 390                 395                 400 

Thr Val Thr Glu Val Glu Glu Val Lys Met Val Val Lys Leu Leu Pro 
                405                 410                 415     

Ile Trp Ser Thr Cys Ile Leu Phe Trp Thr Val Tyr Ser Gln Met Thr 
            420                 425                 430         

Thr Phe Ser Val Glu Gln Ala Thr Arg Met Asp Arg His Leu Arg Pro 
        435                 440                 445             

Gly Ala Ala Pro Gly Gly Phe Ala Ile Pro Ala Gly Ser Leu Ser Val 
    450                 455                 460                 

Phe Leu Phe Leu Ser Ile Leu Leu Phe Thr Ser Leu Asn Glu Arg Val 
465                 470                 475                 480 

Leu Val Pro Ala Ala Arg Arg Leu Thr Arg Arg Pro Gln Gly Leu Thr 
                485                 490                 495     

Ser Leu Gln Arg Val Gly Ala Gly Leu Val Leu Ala Thr Val Ala Met 
            500                 505                 510         

Ala Thr Ser Ala Leu Val Glu Lys Lys Arg Arg Asp Ala Ala Asn Asp 
        515                 520                 525             

Gly Gly Gly Gly Gly Met Ile Ser Ala Phe Trp Leu Val Pro Gln Phe 
    530                 535                 540                 

Phe Leu Val Gly Ala Gly Glu Ala Phe Ala Tyr Val Gly Gln Leu Glu 
545                 550                 555                 560 

Phe Phe Ile Arg Glu Ala Pro Glu Arg Met Lys Ser Met Ser Thr Gly 
                565                 570                 575     

Leu Phe Leu Val Thr Leu Ser Met Gly Phe Phe Leu Ser Ser Phe Leu 
            580                 585                 590         

Val Phe Ala Val Asp Ala Ala Thr Arg Gly Ala Trp Ile Arg Asn Asp 
        595                 600                 605             

Leu Asp Ala Gly Arg Leu Asp Leu Phe Tyr Trp Met Leu Ala Val Leu 
    610                 615                 620                 

Gly Val Ala Asn Phe Ala Val Phe Leu Val Phe Ala Arg Arg His Glu 
625                 630                 635                 640 

Tyr Lys Gln Pro Ala Gly Thr Ala Ala Val Val Val Ala Pro Ala Ala 
                645                 650                 655     

Ala Lys Asp Gly Gly Ala Gly Glu Glu Lys Glu Met Asp Asp Phe Val 
            660                 665                 670         

Val Val Lys Glu Ala Val Glu Gly Met Asp Val 
        675                 680             


<210> 48
<211> 679
<212> PRT
<213> Vitis vinifera

<220>
<221> misc_feature
<223> Public GI ID no. 157348973 

<220>
<221> misc_feature
<222> (1)..(679)
<223> Bit score of 1315.4 for hmm based on sequences of FIGURE 11. 

<220>
<221> misc_feature
<222> (186)..(580)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Functional Homolog Of SEQ ID NO. 2 

<400> 48
Met Ile Ser Pro Lys Cys Met Thr Trp Tyr Lys Leu Tyr Asn His Ser 
1               5                   10                  15      

Asn Pro Asn Phe Cys Ala Thr Thr Arg Cys Gly Arg His Glu Met Pro 
            20                  25                  30          

Thr Asn Ile Arg Leu Leu Ser Tyr Phe Leu Pro Gln Ser Phe Thr Ser 
        35                  40                  45              

Val Tyr Ile Asn Tyr Asn Pro Ser Leu Val Asn Ser Thr Thr Asn His 
    50                  55                  60                  

Pro Ile Ser Ala Leu Leu Ser Leu Ser Leu Ser Leu Ser Leu Ser Pro 
65                  70                  75                  80  

Ser Pro Ser Ala Lys Met Val Leu Val Asp Ala His Ala Gly Lys Asp 
                85                  90                  95      

Gly Thr Asp Asp Gly Ala Leu Asp Phe Arg Gly Asn Pro Val Asp Lys 
            100                 105                 110         

Thr Arg Thr Gly Gly Trp Leu Gly Ala Gly Leu Ile Leu Gly Thr Glu 
        115                 120                 125             

Phe Ala Glu Arg Val Cys Val Met Gly Ile Ser Met Asn Leu Val Thr 
    130                 135                 140                 

Tyr Leu Val Gln Asn Leu His Ile Ser Ala Ala Lys Ser Ala Thr Ile 
145                 150                 155                 160 

Val Thr Asn Phe Met Gly Thr Leu Asn Leu Cys Gly Leu Leu Ala Gly 
                165                 170                 175     

Phe Leu Ala Asp Ala Lys Leu Gly Arg Tyr Leu Thr Val Ala Ile Phe 
            180                 185                 190         

Gly Ser Ile Thr Ala Leu Gly Val Ile Leu Leu Thr Val Ala Thr Thr 
        195                 200                 205             

Ile Pro Ser Met Arg Pro Pro Pro Cys Ser Asp Tyr Met Arg Gln His 
    210                 215                 220                 

His Gln Cys Ile Glu Ala Asn Gly Ser Gln Leu Ala Met Leu Tyr Ala 
225                 230                 235                 240 

Ala Leu Tyr Thr Ile Ala Leu Gly Gly Gly Gly Ile Lys Ser Asn Val 
                245                 250                 255     

Ser Gly Phe Gly Ser Asp Gln Phe Asp Asn Ser Asp Pro Lys Glu Glu 
            260                 265                 270         

Lys Ala Met Ile Phe Phe Phe Asn Arg Phe Tyr Phe Cys Val Ser Ile 
        275                 280                 285             

Gly Ser Leu Phe Ala Val Thr Val Leu Val Tyr Ile Gln Asp Asn Val 
    290                 295                 300                 

Gly Arg Gly Trp Gly Tyr Gly Ile Ser Ala Val Thr Met Val Ile Ala 
305                 310                 315                 320 

Val Thr Ile Leu Leu Gly Gly Thr Pro Phe Tyr Arg Phe Lys Met Pro 
                325                 330                 335     

Arg Gly Ser Pro Leu Thr Val Ile Trp Arg Val Ile Phe Leu Ala Trp 
            340                 345                 350         

Arg Lys Arg Asn Leu Pro Tyr Pro Ser His Pro Ser Leu Leu Asn Glu 
        355                 360                 365             

Tyr His Asn Ala Lys Val Ser His Thr Gln Arg Phe Arg Cys Leu Asp 
    370                 375                 380                 

Lys Ala Ala Ile Leu Glu Asp Tyr Tyr Ser Ala Ser Gly Asn Lys Asp 
385                 390                 395                 400 

Asn Pro Trp Ile Val Ser Thr Val Asn Gln Val Glu Glu Val Lys Met 
                405                 410                 415     

Val Ile Lys Leu Leu Pro Ile Trp Ser Thr Cys Ile Leu Phe Trp Thr 
            420                 425                 430         

Val Tyr Ser Gln Met Thr Thr Phe Thr Ile Glu Gln Ala Thr Phe Met 
        435                 440                 445             

Asn Arg Lys Leu Gly Ser Phe Val Val Pro Ser Gly Ser Phe Ser Val 
    450                 455                 460                 

Phe Leu Phe Ile Ser Ile Leu Leu Phe Thr Ser Leu Asn Glu Arg Ile 
465                 470                 475                 480 

Phe Val Pro Cys Ala Arg Lys Leu Thr His Asn Val Gln Gly Val Thr 
                485                 490                 495     

Ser Leu Gln Arg Val Gly Ile Gly Leu Ile Phe Ser Met Val Ala Met 
            500                 505                 510         

Val Thr Ala Ala Ile Val Glu Lys Gln Arg Arg Glu Ala Ala Val Gln 
        515                 520                 525             

His Lys Thr Asn Ile Ser Ala Phe Trp Leu Val Pro Gln Phe Phe Leu 
    530                 535                 540                 

Val Gly Ala Gly Glu Ala Phe Ala Tyr Val Gly Gln Leu Glu Phe Phe 
545                 550                 555                 560 

Ile Arg Glu Ala Pro Asp Arg Met Lys Ser Met Ser Thr Gly Leu Phe 
                565                 570                 575     

Leu Ser Thr Leu Ala Met Gly Phe Phe Val Ser Ser Leu Leu Val Ser 
            580                 585                 590         

Leu Val Asp Lys Val Thr Asn Met Arg Trp Leu Arg Ser Asn Leu Asn 
        595                 600                 605             

Arg Gly Lys Leu Asp Asn Phe Tyr Trp Met Leu Ala Val Leu Gly Val 
    610                 615                 620                 

Leu Asn Phe Leu Ala Phe Leu Val Phe Ala Met Arg His Gln Tyr Lys 
625                 630                 635                 640 

Thr Leu Gln Tyr Lys Ser Pro Asp Val Tyr Gly Glu Asn Glu Leu Lys 
                645                 650                 655     

Lys Gly Trp Asn Glu Gly Ile Thr Ser Glu Met Glu Lys Lys Glu Arg 
            660                 665                 670         

Val Glu Gly Lys Glu Glu Pro 
        675                 


<210> 49
<211> 545
<212> PRT
<213> Zea mays

<220>
<221> misc_feature
<223> Public GI ID no. 194704154 

<220>
<221> misc_feature
<222> (1)..(545)
<223> Bit score of 1272.2 for hmm based on sequences of FIGURE 11. 

<220>
<221> misc_feature
<222> (51)..(452)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Functional Homolog Of SEQ ID NO. 2 

<400> 49
Met Gly Ile Ser Met Asn Leu Val Thr Tyr Leu Val Gly Glu Leu His 
1               5                   10                  15      

Leu Ser Asn Ser Lys Ser Ala Thr Val Val Thr Asn Phe Met Gly Thr 
            20                  25                  30          

Leu Asn Leu Leu Ala Leu Val Gly Gly Phe Leu Ala Asp Ala Lys Leu 
        35                  40                  45              

Gly Arg Tyr Leu Thr Ile Ala Ile Ser Ala Thr Ile Ala Ala Thr Gly 
    50                  55                  60                  

Val Ser Leu Leu Thr Val Asp Thr Thr Val Pro Ser Met Arg Pro Pro 
65                  70                  75                  80  

Ala Cys Leu Asp Ala Arg Gly Pro Arg Ala His Glu Cys Val Pro Ala 
                85                  90                  95      

Arg Gly Gly Gln Leu Ala Leu Leu Tyr Ala Ala Leu Tyr Thr Val Ala 
            100                 105                 110         

Ala Gly Ala Gly Gly Leu Lys Ala Asn Val Ser Gly Phe Gly Ser Asp 
        115                 120                 125             

Gln Phe Asp Gly Arg Asp Pro Arg Glu Glu Arg Ala Met Val Phe Phe 
    130                 135                 140                 

Phe Asn Arg Phe Tyr Phe Cys Val Ser Leu Gly Ser Leu Phe Ala Val 
145                 150                 155                 160 

Thr Val Leu Val Tyr Val Gln Asp Asn Val Gly Arg Gly Trp Gly Tyr 
                165                 170                 175     

Gly Val Ser Ala Val Ala Met Ala Leu Ala Val Ala Val Leu Val Ala 
            180                 185                 190         

Gly Thr Pro Arg Tyr Arg Tyr Arg Arg Pro Gln Gly Ser Pro Leu Thr 
        195                 200                 205             

Ala Val Gly Arg Val Leu Ala Ala Ala Trp Arg Lys Arg Arg Leu Pro 
    210                 215                 220                 

Leu Pro Ala Asp Ala Ala Glu Leu His Gly Phe Ala Ala Ala Lys Val 
225                 230                 235                 240 

Ala His Thr Asp Arg Leu Arg Trp Leu Asp Lys Ala Ala Ile Val Glu 
                245                 250                 255     

Ala Glu Pro Ala Gly Lys Gln Arg Ala Ser Ala Ala Ala Ala Ser Thr 
            260                 265                 270         

Val Thr Glu Val Glu Glu Val Lys Met Val Ala Lys Leu Leu Pro Ile 
        275                 280                 285             

Trp Ser Thr Cys Ile Leu Phe Trp Thr Val Tyr Ser Gln Met Thr Thr 
    290                 295                 300                 

Phe Ser Val Glu Gln Ala Thr Arg Met Asp Arg His Leu Arg Pro Gly 
305                 310                 315                 320 

Ser Gly Ala Gly Gly Phe Ala Val Pro Ala Gly Ser Phe Ser Val Phe 
                325                 330                 335     

Leu Phe Leu Ser Ile Leu Leu Phe Thr Ser Leu Asn Glu Arg Leu Leu 
            340                 345                 350         

Val Pro Leu Ala Ala Arg Leu Thr Gly Arg Pro Gln Gly Leu Thr Ser 
        355                 360                 365             

Leu Gln Arg Val Gly Ala Gly Leu Ala Leu Ser Val Ala Ala Met Ala 
    370                 375                 380                 

Val Ser Ala Leu Val Glu Arg Lys Arg Arg Asp Ala Ala Asn Gly Pro 
385                 390                 395                 400 

Gly His Val Ala Val Ser Ala Phe Trp Leu Val Pro Gln Tyr Phe Leu 
                405                 410                 415     

Val Gly Ala Gly Glu Ala Phe Ala Tyr Val Gly Gln Leu Glu Phe Phe 
            420                 425                 430         

Ile Arg Glu Ala Pro Glu Arg Met Lys Ser Met Ser Thr Gly Leu Phe 
        435                 440                 445             

Leu Val Thr Leu Ser Met Gly Phe Phe Leu Ser Ser Leu Leu Val Phe 
    450                 455                 460                 

Ala Val Asp Ala Ala Thr Ala Gly Thr Trp Ile Arg Asn Asn Leu Asp 
465                 470                 475                 480 

Arg Gly Arg Leu Asp Leu Phe Tyr Trp Met Leu Ala Leu Leu Gly Val 
                485                 490                 495     

Ala Asn Phe Ala Val Phe Val Val Phe Ala Arg Arg His Gln Tyr Lys 
            500                 505                 510         

Ala Thr Ser Leu Pro Ala Ser Val Ala Pro Asp Gly Thr Gly His Lys 
        515                 520                 525             

Glu Met Asp Asp Phe Val Ala Val Thr Glu Ala Val Glu Gly Val Asp 
    530                 535                 540                 

Val 
545 


<210> 50
<211> 1866
<212> DNA
<213> Sorghum bicolor

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 8666376 

<220>
<221> misc_feature
<223> Encodes the peptide sequence at SEQ ID NO. 51 

<400> 50
atgagcaatg tccgtacagt atgcatatcc atggatcaac aagctaagga ggtttacaca  60    

gtaaaaagac agcaaaataa tggaaggtca gatcaggtcc tgcaggtgca ggagagggca  120   

gcatggggcg ccagcgacgg cggcggcaac ctggtgcagg atgccgtcga ctacaggggc  180   

tgcccggcgg acaagtccag cacgggcggc tgggtggcgg cggcgctcgc cctcgggatc  240   

gagctgtgcg agcgcctctc caccatgggc atcgccgtca acctggtcac ctacctcacg  300   

ggcaccatgc acctgcccag cgccgctgct gccaacgtgg tcaccgactt catgggcacc  360   

tccttcctcc tctgcttcct tggtggattc cttgccgact ccttcctcgg acgatacctc  420   

accatcgcca tcttcgccct cgtccaagcc atcggcacgg ggctgctggc agtgtccacg  480   

gaggtgaggc agctacggcc gccgccgtgc ggatccggcg cggctgggtc gtgcgagcag  540   

gccactgggc tccagatggg cgtcctgtac gtgtgtctct acctcatcgc gctcggcacc  600   

ggtgggctca agtccagcgt ctcgggcttc ggcaccgacc agttcgacga gcgcgacgca  660   

cgcgagcgcg ccgccatggg cctcttcttc aaccgcttct tgttcttcat cagcgtcggc  720   

acgctcctcg ccgtcaccgt cttcgtctac gtccaggacc acgtcggccg gagctgggcc  780   

tatggcatct gcgccggcgc catgctcgtc gccatcgccg tcttcctgtc aggcaccagg  840   

cggtaccgct acaagcgcag ctccgggagc cccatcgtgc acatcctcca ggtcctcgtc  900   

gctgccgccc ggaagcggaa cgtcgtcaag cagccgctca ccgcctccgc gctctacgag  960   

gaccgccccg agcacgcgcg catcccccac accgcccagt tcccgtgcct ggacagggcg  1020  

gcggtgatgg ccggcgacga cgacaacgag gtgggccgcg acgggcggcc gacgcccaac  1080  

ccgtggaagc tgtgcaacgt gtcccgcgtc gaggaggtta agatggtggc gcggctgatg  1140  

ccggtgtggg cgacgaccat cctgttctgg accatctacg cgcagatgat caccttctcc  1200  

gtggagcagg cgacgaccat ggaccggcgc atgggcggct tcgagatccc agccgcctcg  1260  

ctcaccgtct tcttcgtcgg cgctatcatg ctcaccctcg ccttctacga ccgcgtcttc  1320  

gttccgctct gccgcagctt catgaccggc cggcaggggt tcacaaactt ggagaagatc  1380  

ggcatcggcc tggtcctctc catcgtcggc atggccgccg cggcaatctg cgagaagaaa  1440  

cgcctcgccg tcgctgccac gagtcgcggc gcagggcatg ggtcgctgcc catcagcgtg  1500  

ttcatgctga cgccgcagtt cctgctggtg ggcgccggcg aggcgttcat ctacacgggg  1560  

cagctggact tcttcatcac gcggtcgccc aagagcatga agacgatgag cacgggcctc  1620  

ttcctcacca cgctctcgct cggcttcttc ctcagcagcg cgctcgtctc gctcgtcaag  1680  

agctgcacgc ggtggctcgg cgacaccatc aaccacagcc gcctcgacta cttctactgg  1740  

ctgctggccg tgctcggcgt tgtcaacctc gtcgcatacc tcgtctgcgc catgtgggca  1800  

acagcaccgg ccagcaggca ggcggagcag ccgcagtccg ccatggccgc cgacgagaaa  1860  

tgctag                                                             1866  


<210> 51
<211> 621
<212> PRT
<213> Sorghum bicolor

<220>
<221> misc_feature
<223> Ceres ANNOT ID no. 8666376 

<220>
<221> misc_feature
<222> (1)..(621)
<223> Bit score of 1512.3 for hmm based on sequences of FIGURE 12. 

<220>
<221> misc_feature
<222> (139)..(545)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Functional Homolog Of SEQ ID NO. 17 

<400> 51
Met Ser Asn Val Arg Thr Val Cys Ile Ser Met Asp Gln Gln Ala Lys 
1               5                   10                  15      

Glu Val Tyr Thr Val Lys Arg Gln Gln Asn Asn Gly Arg Ser Asp Gln 
            20                  25                  30          

Val Leu Gln Val Gln Glu Arg Ala Ala Trp Gly Ala Ser Asp Gly Gly 
        35                  40                  45              

Gly Asn Leu Val Gln Asp Ala Val Asp Tyr Arg Gly Cys Pro Ala Asp 
    50                  55                  60                  

Lys Ser Ser Thr Gly Gly Trp Val Ala Ala Ala Leu Ala Leu Gly Ile 
65                  70                  75                  80  

Glu Leu Cys Glu Arg Leu Ser Thr Met Gly Ile Ala Val Asn Leu Val 
                85                  90                  95      

Thr Tyr Leu Thr Gly Thr Met His Leu Pro Ser Ala Ala Ala Ala Asn 
            100                 105                 110         

Val Val Thr Asp Phe Met Gly Thr Ser Phe Leu Leu Cys Phe Leu Gly 
        115                 120                 125             

Gly Phe Leu Ala Asp Ser Phe Leu Gly Arg Tyr Leu Thr Ile Ala Ile 
    130                 135                 140                 

Phe Ala Leu Val Gln Ala Ile Gly Thr Gly Leu Leu Ala Val Ser Thr 
145                 150                 155                 160 

Glu Val Arg Gln Leu Arg Pro Pro Pro Cys Gly Ser Gly Ala Ala Gly 
                165                 170                 175     

Ser Cys Glu Gln Ala Thr Gly Leu Gln Met Gly Val Leu Tyr Val Cys 
            180                 185                 190         

Leu Tyr Leu Ile Ala Leu Gly Thr Gly Gly Leu Lys Ser Ser Val Ser 
        195                 200                 205             

Gly Phe Gly Thr Asp Gln Phe Asp Glu Arg Asp Ala Arg Glu Arg Ala 
    210                 215                 220                 

Ala Met Gly Leu Phe Phe Asn Arg Phe Leu Phe Phe Ile Ser Val Gly 
225                 230                 235                 240 

Thr Leu Leu Ala Val Thr Val Phe Val Tyr Val Gln Asp His Val Gly 
                245                 250                 255     

Arg Ser Trp Ala Tyr Gly Ile Cys Ala Gly Ala Met Leu Val Ala Ile 
            260                 265                 270         

Ala Val Phe Leu Ser Gly Thr Arg Arg Tyr Arg Tyr Lys Arg Ser Ser 
        275                 280                 285             

Gly Ser Pro Ile Val His Ile Leu Gln Val Leu Val Ala Ala Ala Arg 
    290                 295                 300                 

Lys Arg Asn Val Val Lys Gln Pro Leu Thr Ala Ser Ala Leu Tyr Glu 
305                 310                 315                 320 

Asp Arg Pro Glu His Ala Arg Ile Pro His Thr Ala Gln Phe Pro Cys 
                325                 330                 335     

Leu Asp Arg Ala Ala Val Met Ala Gly Asp Asp Asp Asn Glu Val Gly 
            340                 345                 350         

Arg Asp Gly Arg Pro Thr Pro Asn Pro Trp Lys Leu Cys Asn Val Ser 
        355                 360                 365             

Arg Val Glu Glu Val Lys Met Val Ala Arg Leu Met Pro Val Trp Ala 
    370                 375                 380                 

Thr Thr Ile Leu Phe Trp Thr Ile Tyr Ala Gln Met Ile Thr Phe Ser 
385                 390                 395                 400 

Val Glu Gln Ala Thr Thr Met Asp Arg Arg Met Gly Gly Phe Glu Ile 
                405                 410                 415     

Pro Ala Ala Ser Leu Thr Val Phe Phe Val Gly Ala Ile Met Leu Thr 
            420                 425                 430         

Leu Ala Phe Tyr Asp Arg Val Phe Val Pro Leu Cys Arg Ser Phe Met 
        435                 440                 445             

Thr Gly Arg Gln Gly Phe Thr Asn Leu Glu Lys Ile Gly Ile Gly Leu 
    450                 455                 460                 

Val Leu Ser Ile Val Gly Met Ala Ala Ala Ala Ile Cys Glu Lys Lys 
465                 470                 475                 480 

Arg Leu Ala Val Ala Ala Thr Ser Arg Gly Ala Gly His Gly Ser Leu 
                485                 490                 495     

Pro Ile Ser Val Phe Met Leu Thr Pro Gln Phe Leu Leu Val Gly Ala 
            500                 505                 510         

Gly Glu Ala Phe Ile Tyr Thr Gly Gln Leu Asp Phe Phe Ile Thr Arg 
        515                 520                 525             

Ser Pro Lys Ser Met Lys Thr Met Ser Thr Gly Leu Phe Leu Thr Thr 
    530                 535                 540                 

Leu Ser Leu Gly Phe Phe Leu Ser Ser Ala Leu Val Ser Leu Val Lys 
545                 550                 555                 560 

Ser Cys Thr Arg Trp Leu Gly Asp Thr Ile Asn His Ser Arg Leu Asp 
                565                 570                 575     

Tyr Phe Tyr Trp Leu Leu Ala Val Leu Gly Val Val Asn Leu Val Ala 
            580                 585                 590         

Tyr Leu Val Cys Ala Met Trp Ala Thr Ala Pro Ala Ser Arg Gln Ala 
        595                 600                 605             

Glu Gln Pro Gln Ser Ala Met Ala Ala Asp Glu Lys Cys 
    610                 615                 620     


<210> 52
<211> 566
<212> PRT
<213> Oryza sativa subsp. indica

<220>
<221> misc_feature
<223> Public GI ID no. 125526400 

<220>
<221> misc_feature
<222> (1)..(566)
<223> Bit score of 1476.3 for hmm based on sequences of FIGURE 12. 

<220>
<221> misc_feature
<222> (89)..(487)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Functional Homolog Of SEQ ID NO. 17 

<400> 52
Met Val Arg Asp Ala Val Asp Tyr Arg Gly Cys Pro Ala Asp Arg Ser 
1               5                   10                  15      

Ala Thr Gly Gly Trp Val Ala Ala Ala Leu Val Leu Gly Ile Glu Leu 
            20                  25                  30          

Cys Glu Arg Leu Ser Thr Met Gly Ile Ala Val Asn Leu Val Thr Tyr 
        35                  40                  45              

Leu Thr Gly Thr Met His Leu Pro Ser Ala Ala Ala Ala Asn Val Val 
    50                  55                  60                  

Thr Asp Phe Met Gly Thr Ser Phe Leu Leu Cys Leu Leu Gly Gly Phe 
65                  70                  75                  80  

Leu Ala Asp Ser Phe Leu Gly Arg Tyr Leu Thr Ile Ala Val Phe Ala 
                85                  90                  95      

Leu Val Gln Ser Ile Gly Thr Ala Leu Leu Ala Ala Ser Thr Leu Val 
            100                 105                 110         

Thr His Leu Arg Pro Pro Pro Gly Glu Gln Ala Thr Pro Val Gln Met 
        115                 120                 125             

Ala Val Leu Tyr Ala Cys Leu Tyr Leu Ile Ala Leu Gly Thr Gly Gly 
    130                 135                 140                 

Leu Lys Ser Ser Val Ser Gly Phe Gly Thr Asp Gln Phe Asp Asp Arg 
145                 150                 155                 160 

Asp Gly Arg Glu Arg Ala Asp Met Gly Leu Phe Phe Asn Arg Phe Phe 
                165                 170                 175     

Phe Phe Ile Ser Leu Gly Thr Leu Leu Ala Val Thr Val Leu Val Tyr 
            180                 185                 190         

Val Gln Asp His Val Gly Arg Ser Trp Ala Tyr Gly Ile Cys Ser Ala 
        195                 200                 205             

Ala Met Leu Ala Ala Ile Ala Val Phe Leu Ser Gly Thr Arg Arg Tyr 
    210                 215                 220                 

Arg Tyr Lys Arg Ser Ser Gly Ser Pro Ile Val His Ile Leu Gln Val 
225                 230                 235                 240 

Leu Val Ala Ala Ala Arg Lys Arg Gly Val Val Lys Arg Pro Pro Thr 
                245                 250                 255     

Ala Ala Glu Leu Tyr Glu Asp Asp Arg Pro Glu His Ala Arg Ile Ala 
            260                 265                 270         

His Thr Ala Gln Phe Pro Cys Leu Asp Met Ala Ala Val Val Ala Gly 
        275                 280                 285             

Glu Glu Asp Asn Glu Val Ala Gly Pro Gly Gly Pro Ala Ala Pro Asn 
    290                 295                 300                 

Pro Trp Lys Leu Cys Ser Val Ser Arg Val Glu Glu Val Lys Met Val 
305                 310                 315                 320 

Ala Arg Leu Met Pro Val Trp Ala Thr Thr Ile Leu Phe Trp Thr Ile 
                325                 330                 335     

Tyr Ala Gln Met Ile Thr Phe Ser Val Glu Gln Ala Thr Thr Met Asp 
            340                 345                 350         

Arg Arg Val Gly Ala Gly Phe Glu Ile Pro Ala Ala Ser Leu Thr Val 
        355                 360                 365             

Phe Phe Val Gly Ala Ile Met Leu Thr Leu Ala Val Tyr Asp Arg Val 
    370                 375                 380                 

Phe Ile Pro Leu Cys Arg Val Leu Thr Gly Lys Gln Gly Phe Thr Asn 
385                 390                 395                 400 

Leu Glu Lys Ile Gly Ile Gly Leu Ala Leu Ser Ile Leu Gly Met Ala 
                405                 410                 415     

Ala Ala Ala Leu Cys Glu Lys Lys Arg Leu Ala Val Ala Val Ala Ala 
            420                 425                 430         

Thr Thr Gly Asn Ser Thr Pro Ile Ser Val Phe Leu Leu Thr Pro Gln 
        435                 440                 445             

Phe Leu Leu Val Gly Ala Gly Glu Ala Phe Ile Tyr Thr Gly Gln Leu 
    450                 455                 460                 

Asp Phe Phe Ile Thr Arg Ser Pro Lys Gly Met Lys Thr Met Ser Thr 
465                 470                 475                 480 

Gly Leu Phe Leu Thr Thr Leu Ser Leu Gly Phe Phe Phe Ser Ser Val 
                485                 490                 495     

Leu Val Ser Leu Val Lys Gly Ala Thr Thr Trp Leu Gly Asp Thr Ile 
            500                 505                 510         

Asp Arg Ser Arg Leu Asp Tyr Phe Tyr Trp Leu Leu Ala Val Leu Ser 
        515                 520                 525             

Val Leu Asn Leu Ala Ala Tyr Leu Val Cys Ala Lys Trp Ala Ala Thr 
    530                 535                 540                 

Ala Ala Ala Thr Ser Arg Glu Gln Gln Gln Gln His Thr Ala Val Ala 
545                 550                 555                 560 

Asp Ala Asp Glu Lys Cys 
                565     


<210> 53
<211> 576
<212> PRT
<213> Vitis vinifera

<220>
<221> misc_feature
<223> Public GI ID no. 157342940 

<220>
<221> misc_feature
<222> (1)..(576)
<223> Bit score of 1449.7 for hmm based on sequences of FIGURE 12. 

<220>
<221> misc_feature
<222> (92)..(491)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Functional Homolog Of SEQ ID NO. 17 

<400> 53
Met Ser Trp Thr Val Ala Asp Ala Val Asp Tyr Lys Gly Phe Pro Ala 
1               5                   10                  15      

Asp Arg Ser Lys Thr Gly Gly Trp Val Pro Ala Ala Leu Ile Leu Gly 
            20                  25                  30          

Ile Glu Ile Cys Glu Arg Leu Ser Thr Met Gly Ile Ala Val Asn Leu 
        35                  40                  45              

Val Thr Tyr Leu Val Ser Thr Met His Leu Pro Ser Ala Thr Ser Ala 
    50                  55                  60                  

Asn Thr Val Thr Asp Phe Met Gly Thr Ser Phe Leu Leu Cys Leu Leu 
65                  70                  75                  80  

Gly Gly Phe Leu Ala Asp Ser Phe Leu Gly Arg Phe Arg Thr Ile Ala 
                85                  90                  95      

Ile Phe Ala Thr Val Gln Ala Met Gly Thr Gly Met Leu Ala Ile Ile 
            100                 105                 110         

Thr Arg Leu Pro Gln Leu Gln Pro Pro Pro Cys Lys Asn Thr Pro Thr 
        115                 120                 125             

Gln Thr Cys Lys Gln Ala Asn Gly Phe Gln Met Gly Ile Leu Tyr Leu 
    130                 135                 140                 

Ala Leu Tyr Leu Ile Ala Leu Gly Thr Gly Gly Leu Lys Ser Ser Val 
145                 150                 155                 160 

Ser Gly Phe Gly Thr Asp Gln Phe Asp Asp Lys Asp Glu Lys Glu Gln 
                165                 170                 175     

Ser Gln Met Ile Tyr Phe Phe Ser Arg Phe Phe Phe Phe Ile Ser Thr 
            180                 185                 190         

Gly Thr Leu Met Ala Val Thr Val Leu Val Tyr Ile Gln Asp Glu Val 
        195                 200                 205             

Ser Arg Ser Leu Gly Tyr Gly Ile Cys Cys Val Ser Met Leu Ile Ala 
    210                 215                 220                 

Ile Leu Val Tyr Phe Ser Gly Thr Lys Arg Tyr Arg Tyr Lys Lys Ser 
225                 230                 235                 240 

Val Gly Ser Pro Ile Val His Ile Ser Gln Val Ile Val Ala Ala Met 
                245                 250                 255     

Arg Lys Arg Lys Met Asp Leu Pro Phe Asn Ala Ser Leu Leu Tyr Glu 
            260                 265                 270         

Asp Ser Ala Glu Gly Ser Arg Ile Gln His Thr Asp Gln Phe Arg Cys 
        275                 280                 285             

Leu Asp Lys Ala Ala Ile Leu Ala Gly Glu Phe Asp Lys Thr Ala Ser 
    290                 295                 300                 

Ser Asp Pro Asn Pro Trp Lys Leu Cys Ser Val Thr Arg Val Glu Glu 
305                 310                 315                 320 

Val Lys Met Met Ala Arg Leu Leu Pro Ile Trp Ala Thr Thr Ile Ile 
                325                 330                 335     

Phe Trp Thr Thr Tyr Ala Gln Met Ile Thr Phe Ser Val Glu Gln Ala 
            340                 345                 350         

Ser Thr Met Glu Arg Ser Ile Gly Asn Phe Gln Ile Pro Ala Gly Ser 
        355                 360                 365             

Leu Thr Val Phe Phe Val Ala Ala Ile Leu Ile Thr Leu Ala Val Tyr 
    370                 375                 380                 

Asp Arg Phe Ile Met Pro Met Trp Lys Lys Trp Arg Gly Lys Pro Gly 
385                 390                 395                 400 

Phe Ser Ser Leu Gln Arg Ile Ala Leu Gly Leu Ile Leu Ser Ile Val 
                405                 410                 415     

Gly Met Thr Ala Ala Ala Leu Ala Glu Arg Arg Arg Leu Ser Val Ala 
            420                 425                 430         

Lys Ala Val Gly Asp Asn Thr Ala Thr Leu Pro Ile Ser Val Phe Leu 
        435                 440                 445             

Leu Ile Pro Gln Phe Phe Leu Val Gly Ser Gly Glu Ala Phe Ile Tyr 
    450                 455                 460                 

Thr Gly Gln Leu Asp Phe Phe Ile Thr Gln Ser Pro Lys Gly Met Lys 
465                 470                 475                 480 

Thr Met Ser Thr Gly Leu Phe Leu Ser Thr Leu Ser Leu Gly Phe Phe 
                485                 490                 495     

Val Ser Ser Phe Leu Val Ser Val Val Lys Arg Val Thr Gly Ser Arg 
            500                 505                 510         

Asp Gly Gln Gly Trp Leu Ala Asp Asn Ile Asn Tyr Gly Arg Leu Asp 
        515                 520                 525             

Cys Phe Tyr Gly Leu Leu Ala Ile Leu Ser Phe Ile Asn Phe Ala Val 
    530                 535                 540                 

Phe Leu Val Cys Ala Val Trp Tyr Lys Pro Gln Lys Pro Asn Asp Gly 
545                 550                 555                 560 

Met Gln Met Glu Thr Met Ala Asn Gly Ser Cys Pro Glu Glu Lys Cys 
                565                 570                 575     


<210> 54
<211> 594
<212> PRT
<213> Nicotiana tabacum

<220>
<221> misc_feature
<223> Public GI ID no. 28273098 

<220>
<221> misc_feature
<222> (1)..(594)
<223> Bit score of 1488.0 for hmm based on sequences of FIGURE 12. 

<220>
<221> misc_feature
<222> (100)..(513)
<223> Pfam Name: PTR2 
      Pfam Description: POT family 

<220>
<221> misc_feature
<223> Functional Homolog Of SEQ ID NO. 17 

<400> 54
Met Ala Leu Pro Glu Thr Gln Gln Asp Ser Lys Ala Leu Pro Asp Ala 
1               5                   10                  15      

Trp Asp Tyr Lys Gly Arg Pro Ala Leu Arg Ser Ser Ser Gly Gly Trp 
            20                  25                  30          

Ala Ser Gly Ala Met Ile Leu Gly Val Glu Ala Val Glu Arg Leu Thr 
        35                  40                  45              

Thr Leu Gly Ile Ala Val Asn Leu Val Thr Tyr Leu Thr Gly Thr Met 
    50                  55                  60                  

His Leu Gly Asn Ala Thr Ala Ala Asn Asn Val Thr Asn Phe Leu Gly 
65                  70                  75                  80  

Thr Ser Phe Met Leu Thr Leu Phe Gly Gly Phe Val Ala Asp Thr Phe 
                85                  90                  95      

Leu Gly Arg Tyr Leu Thr Ile Gly Ile Phe Ala Thr Val Gln Ala Met 
            100                 105                 110         

Gly Val Thr Ile Leu Thr Ile Ser Thr Ile Ile Pro Ser Leu Arg Pro 
        115                 120                 125             

Pro Lys Cys Glu Gln Val Gly Ser Ser Ser Cys Ile Pro Ala Asn Ser 
    130                 135                 140                 

Lys Gln Leu Met Val Leu Tyr Ile Ala Leu Tyr Met Thr Ala Leu Gly 
145                 150                 155                 160 

Thr Gly Gly Leu Lys Ser Ser Val Ser Gly Phe Gly Thr Asp Gln Phe 
                165                 170                 175     

Asp Asp Ala Asp Lys Lys Glu Lys Gly Gln Met Ile Lys Phe Phe Asp 
            180                 185                 190         

Trp Phe Phe Phe Phe Ile Asn Val Gly Ser Leu Gly Ala Val Thr Val 
        195                 200                 205             

Leu Val Tyr Ile Gln Asp Asn Leu Gly Arg Glu Trp Gly Tyr Gly Ile 
    210                 215                 220                 

Cys Ala Cys Ala Ile Val Ile Gly Leu Val Val Phe Leu Ser Gly Thr 
225                 230                 235                 240 

Arg Lys Tyr Arg Phe Lys Lys Leu Val Gly Ser Pro Leu Thr Gln Ile 
                245                 250                 255     

Ala Ser Val Ile Val Ala Ala Trp Lys Lys Arg His Leu Glu Leu Leu 
            260                 265                 270         

Ser Asp Ser Ser Leu Leu Phe Glu Ile Asp Asp Ile Phe Gly Glu Gly 
        275                 280                 285             

Asn Lys Lys Asn Lys Gln Lys Leu Pro His Ser Lys Glu Tyr Arg Phe 
    290                 295                 300                 

Leu Asp Lys Ala Ala Ile Lys Glu Asp His Asp Leu Glu Ser Asn Gly 
305                 310                 315                 320 

Thr Asn Val Val Ile Asn Lys Trp Lys Leu Ala Thr Leu Thr Asp Val 
                325                 330                 335     

Glu Glu Val Lys Leu Leu Ile Arg Met Leu Pro Thr Trp Ala Thr Thr 
            340                 345                 350         

Ile Met Phe Trp Thr Val Tyr Ala Gln Met Thr Thr Phe Ser Val Ser 
        355                 360                 365             

Gln Ala Thr Thr Met Asp Arg His Ile Gly Thr Phe Glu Ile Pro Pro 
    370                 375                 380                 

Ala Ser Leu Thr Val Phe Phe Val Gly Ser Ile Leu Leu Thr Val Ile 
385                 390                 395                 400 

Phe Tyr Asp Arg Val Ile Val Pro Ile Cys Arg Arg Phe Met Asn Lys 
                405                 410                 415     

Pro His Gly Leu Thr Pro Leu Gln Arg Ile Phe Thr Gly Leu Val Leu 
            420                 425                 430         

Ser Ile Leu Ala Met Ile Ala Ala Ala Leu Thr Glu Val Lys Arg Leu 
        435                 440                 445             

Lys Val Ala His Leu His Gly Leu Thr Asn Asp Ala Asn Ala Thr Ile 
    450                 455                 460                 

Pro Leu Ser Val Phe Trp Leu Val Pro Gln Phe Leu Leu Val Gly Ala 
465                 470                 475                 480 

Gly Glu Ala Phe Thr Tyr Ile Gly Gln Leu Asp Phe Phe Leu Arg Glu 
                485                 490                 495     

Cys Pro Lys Gly Met Lys Thr Met Ser Thr Gly Leu Phe Leu Ser Thr 
            500                 505                 510         

Leu Ala Leu Gly Phe Phe Phe Ser Ser Ile Leu Val Thr Ile Val His 
        515                 520                 525             

Val Val Thr Gly Thr Thr Asn Pro Trp Leu Ala Asp Asn Leu Asn Gln 
    530                 535                 540                 

Gly Arg Leu Tyr Asp Phe Tyr Trp Leu Leu Ala Ile Leu Ser Val Leu 
545                 550                 555                 560 

Asn Leu Met Phe Phe Leu Tyr Phe Ser Arg Lys Tyr Val Tyr Lys Glu 
                565                 570                 575     

Lys Arg Leu Ala Glu Met Gly Ile Glu Leu Glu Asp Asp Gly Pro Val 
            580                 585                 590         

Cys His 
        


