                         SEQUENCE LISTING

<110>  The Henry M. Jackson Foundation For The Advancement Of 
       Military Medicine
 
<120>  HIV-1 Envelope Glycoprotein Oligomer and Methods of Use

<130>  044508-5021-WO

<150>  US 60/987,287
<151>  2007-11-12

<160>  13    

<170>  PatentIn version 3.4

<210>  1
<211>  2079
<212>  DNA
<213>  Human immunodeficiency virus

<400>  1
atgcgcgtga aggggatccg ccgcaactac cagcactggt ggggctgggg caccatgctg     60

ctgggcctgc tgatgatctg cagcgccacc gagaagctgt gggtgaccgt gtactacggc    120

gtgcccgtgt ggaaggaggc caccaccacc ctgttctgcg ccagcgacgc caaggcctac    180

gacaccgagg cccacaacgt gtgggccacc cacgcctgcg tgcccaccga ccccaacccc    240

caggaggtgg agctggtgaa cgtgaccgag aacttcaaca tgtggaagaa caacatggtg    300

gagcagatgc acgaggacat catcagcctg tgggaccaga gcctgaagcc ctgcgtgaag    360

ctgacccccc tgtgcgtgac cctgaactgc accgacctgc gcaacaccac caacaccaac    420

aacagcaccg acaacaacaa cagcaacagc gagggcacca tcaagggcgg cgagatgaag    480

aactgcagct tcaacatcgc cacctccatc ggcgacaaga tgcagaagga gtacgccctg    540

ctgtacaagc tggacatcga gcccatcgac aacgacaaca ccagctaccg cctgatcagc    600

tgcaacacca gcgtgatcac ccaggcctgc cccaagatca gcttcgagcc catccccatc    660

cactactgcg cccccgccgg cttcgccatc ctgaagtgca acgacaagaa gttcagcggc    720

aagggcagct gcaagaacgt gagcaccgtg cagtgcaccc acggcatccg ccccgtggtg    780

agcacccagc tgctgctgaa cggcagcctg gccgaggagg aggtggtgat ccgcagcgag    840

aacttcacca acaacgccaa gaccatcatc gtgcagctgc gcgagcccgt gaagatcaac    900

tgcagccgcc ccaacaacaa cacccgcaag agcatcccca tgggccccgg ccgcgccttc    960

tacaccaccg gccagatcat cggcgacatc cgccaggccc actgcaacat cagcaagacc   1020

aactggacca acgccctgaa gcaggtggtg gagaagctgg gcgagcagtt caacaagacc   1080

aagatcgtgt tcaccaacag cagcggcggc gacccggaga tcgtgaccca cagcttcaac   1140

tgcgccggcg agttcttcta ctgcaacacc acccagctgt tcgacagcat ctggaacagc   1200

gagaacggca cctggaacat cacccgcggc ctgaacaaca ccggccgcaa cgacaccatc   1260

accctgccct gccgcatcaa gcagatcatc aaccgctggc aggaagtggg caaggccatg   1320

tacgcccctc ccatcaaggg caacatcagc tgcagcagca acatcaccgg cctgctgctg   1380

acccgcgacg gcggcaagga cgacaacagc cgcgacggca acgagacctt ccgccccggc   1440

ggcggcgaca tgcgcgacaa ctggcgcagc gagctgtaca agtacaaggt ggtgaagatc   1500

gagcccctgg gcgtggcccc caccaaggcc aagcgacgcg tggtgcagag cgaggagagc   1560

gctgtgggcc tgggcgctat gttcatcggc ttcctgggcg ctgctggcag caccatgggc   1620

gctgctagcg tgaccctgac cgtgcaggct cggcagctgc tcagcggcat cgtgcagcag   1680

cagagcaacc tgctccgggc tatcgaagcc cagcagcacc tgctgcagct caccgtgtgg   1740

ggcatcaaac agctgcaggc tcggatcctg gctgtggagc ggtacctgaa agatcagcag   1800

ctgctcggca tctggggctg cagcggcaaa ctgatctgca ccaccaccgt gccctggaac   1860

gccagctgga gcaagaacaa gaccctggag gccatctgga acaacatgac ctggatgcag   1920

tgggacaagg agatcgacaa ctacaccagc ctgatctaca gcctgatcga ggaaagccag   1980

atccagcagg agaagaacga acaggaactg ctggagctgg acaagtgggc caacctgtgg   2040

aactggttcg acatcagcaa ctggctgtgg tacatcaag                          2079


<210>  2
<211>  693
<212>  PRT
<213>  Human immunodeficiency virus

<400>  2

Met Arg Val Lys Gly Ile Arg Arg Asn Tyr Gln His Trp Trp Gly Trp 
1               5                   10                  15      


Gly Thr Met Leu Leu Gly Leu Leu Met Ile Cys Ser Ala Thr Glu Lys 
            20                  25                  30          


Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr 
        35                  40                  45              


Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu Ala 
    50                  55                  60                  


His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro 
65                  70                  75                  80  


Gln Glu Val Glu Leu Val Asn Val Thr Glu Asn Phe Asn Met Trp Lys 
                85                  90                  95      


Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp 
            100                 105                 110         


Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu 
        115                 120                 125             


Asn Cys Thr Asp Leu Arg Asn Thr Thr Asn Thr Asn Asn Ser Thr Asp 
    130                 135                 140                 


Asn Asn Asn Ser Asn Ser Glu Gly Thr Ile Lys Gly Gly Glu Met Lys 
145                 150                 155                 160 


Asn Cys Ser Phe Asn Ile Ala Thr Ser Ile Gly Asp Lys Met Gln Lys 
                165                 170                 175     


Glu Tyr Ala Leu Leu Tyr Lys Leu Asp Ile Glu Pro Ile Asp Asn Asp 
            180                 185                 190         


Asn Thr Ser Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln 
        195                 200                 205             


Ala Cys Pro Lys Ile Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala 
    210                 215                 220                 


Pro Ala Gly Phe Ala Ile Leu Lys Cys Asn Asp Lys Lys Phe Ser Gly 
225                 230                 235                 240 


Lys Gly Ser Cys Lys Asn Val Ser Thr Val Gln Cys Thr His Gly Ile 
                245                 250                 255     


Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu 
            260                 265                 270         


Glu Glu Val Val Ile Arg Ser Glu Asn Phe Thr Asn Asn Ala Lys Thr 
        275                 280                 285             


Ile Ile Val Gln Leu Arg Glu Pro Val Lys Ile Asn Cys Ser Arg Pro 
    290                 295                 300                 


Asn Asn Asn Thr Arg Lys Ser Ile Pro Met Gly Pro Gly Arg Ala Phe 
305                 310                 315                 320 


Tyr Thr Thr Gly Gln Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn 
                325                 330                 335     


Ile Ser Lys Thr Asn Trp Thr Asn Ala Leu Lys Gln Val Val Glu Lys 
            340                 345                 350         


Leu Gly Glu Gln Phe Asn Lys Thr Lys Ile Val Phe Thr Asn Ser Ser 
        355                 360                 365             


Gly Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Ala Gly Glu 
    370                 375                 380                 


Phe Phe Tyr Cys Asn Thr Thr Gln Leu Phe Asp Ser Ile Trp Asn Ser 
385                 390                 395                 400 


Glu Asn Gly Thr Trp Asn Ile Thr Arg Gly Leu Asn Asn Thr Gly Arg 
                405                 410                 415     


Asn Asp Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Arg 
            420                 425                 430         


Trp Gln Glu Val Gly Lys Ala Met Tyr Ala Pro Pro Ile Lys Gly Asn 
        435                 440                 445             


Ile Ser Cys Ser Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly 
    450                 455                 460                 


Gly Lys Asp Asp Asn Ser Arg Asp Gly Asn Glu Thr Phe Arg Pro Gly 
465                 470                 475                 480 


Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys 
                485                 490                 495     


Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg 
            500                 505                 510         


Arg Val Val Gln Ser Glu Glu Ser Ala Val Gly Leu Gly Ala Met Phe 
        515                 520                 525             


Ile Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Val 
    530                 535                 540                 


Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
545                 550                 555                 560 


Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
                565                 570                 575     


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val 
            580                 585                 590         


Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser 
        595                 600                 605             


Gly Lys Leu Ile Cys Thr Thr Thr Val Pro Trp Asn Ala Ser Trp Ser 
    610                 615                 620                 


Lys Asn Lys Thr Leu Glu Ala Ile Trp Asn Asn Met Thr Trp Met Gln 
625                 630                 635                 640 


Trp Asp Lys Glu Ile Asp Asn Tyr Thr Ser Leu Ile Tyr Ser Leu Ile 
                645                 650                 655     


Glu Glu Ser Gln Ile Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu 
            660                 665                 670         


Leu Asp Lys Trp Ala Asn Leu Trp Asn Trp Phe Asp Ile Ser Asn Trp 
        675                 680                 685             


Leu Trp Tyr Ile Lys 
    690             


<210>  3
<211>  2214
<212>  DNA
<213>  Artificial

<220>
<223>  Fusion of truncated HIV with GCN4 coiled coil

<400>  3
atgcgcgtga aggggatccg ccgcaactac cagcactggt ggggctgggg caccatgctg     60

ctgggcctgc tgatgatctg cagcgccacc gagaagctgt gggtgaccgt gtactacggc    120

gtgcccgtgt ggaaggaggc caccaccacc ctgttctgcg ccagcgacgc caaggcctac    180

gacaccgagg cccacaacgt gtgggccacc cacgcctgcg tgcccaccga ccccaacccc    240

caggaggtgg agctggtgaa cgtgaccgag aacttcaaca tgtggaagaa caacatggtg    300

gagcagatgc acgaggacat catcagcctg tgggaccaga gcctgaagcc ctgcgtgaag    360

ctgacccccc tgtgcgtgac cctgaactgc accgacctgc gcaacaccac caacaccaac    420

aacagcaccg acaacaacaa cagcaacagc gagggcacca tcaagggcgg cgagatgaag    480

aactgcagct tcaacatcgc cacctccatc ggcgacaaga tgcagaagga gtacgccctg    540

ctgtacaagc tggacatcga gcccatcgac aacgacaaca ccagctaccg cctgatcagc    600

tgcaacacca gcgtgatcac ccaggcctgc cccaagatca gcttcgagcc catccccatc    660

cactactgcg cccccgccgg cttcgccatc ctgaagtgca acgacaagaa gttcagcggc    720

aagggcagct gcaagaacgt gagcaccgtg cagtgcaccc acggcatccg ccccgtggtg    780

agcacccagc tgctgctgaa cggcagcctg gccgaggagg aggtggtgat ccgcagcgag    840

aacttcacca acaacgccaa gaccatcatc gtgcagctgc gcgagcccgt gaagatcaac    900

tgcagccgcc ccaacaacaa cacccgcaag agcatcccca tgggccccgg ccgcgccttc    960

tacaccaccg gccagatcat cggcgacatc cgccaggccc actgcaacat cagcaagacc   1020

aactggacca acgccctgaa gcaggtggtg gagaagctgg gcgagcagtt caacaagacc   1080

aagatcgtgt tcaccaacag cagcggcggc gacccggaga tcgtgaccca cagcttcaac   1140

tgcgccggcg agttcttcta ctgcaacacc acccagctgt tcgacagcat ctggaacagc   1200

gagaacggca cctggaacat cacccgcggc ctgaacaaca ccggccgcaa cgacaccatc   1260

accctgccct gccgcatcaa gcagatcatc aaccgctggc aggaagtggg caaggccatg   1320

tacgcccctc ccatcaaggg caacatcagc tgcagcagca acatcaccgg cctgctgctg   1380

acccgcgacg gcggcaagga cgacaacagc cgcgacggca acgagacctt ccgccccggc   1440

ggcggcgaca tgcgcgacaa ctggcgcagc gagctgtaca agtacaaggt ggtgaagatc   1500

gagcccctgg gcgtggcccc caccaaggcc aagcgacgcg tggtgcagag cgaggagagc   1560

gctgtgggcc tgggcgctat gttcatcggc ttcctgggcg ctgctggcag caccatgggc   1620

gctgctagcg tgaccctgac cgtgcaggct cggcagctgc tcagcggcat cgtgcagcag   1680

cagagcaacc tgctccgggc tatcgaagcc cagcagcacc tgctgcagct caccgtgtgg   1740

ggcatcaaac agctgcaggc tcggatcctg gctgtggagc ggtacctgaa agatcagcag   1800

ctgctcggca tctggggctg cagcggcaaa ctgatctgca ccaccaccgt gccctggaac   1860

gccagctgga gcaagaacaa gaccctggag gccatctgga acaacatgac ctggatgcag   1920

tgggacaagg agatcgacaa ctacaccagc ctgatctaca gcctgatcga ggaaagccag   1980

atccagcagg agaagaacga acaggaactg ctggagctgg acaagtgggc caacctgtgg   2040

aactggttcg acatcagcaa ctggctgtgg tacatcaaga agcttatgaa gcagatcgag   2100

gacaagatcg aggagatcct gagcaagatc taccacatcg agaacgagat cgccaggatc   2160

aagaagctga tcggcgaggc ccctggcggc atcgagggca ggaagcttta ataa         2214


<210>  4
<211>  736
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion of truncated HIV with GCN4 coiled coil

<400>  4

Met Arg Val Lys Gly Ile Arg Arg Asn Tyr Gln His Trp Trp Gly Trp 
1               5                   10                  15      


Gly Thr Met Leu Leu Gly Leu Leu Met Ile Cys Ser Ala Thr Glu Lys 
            20                  25                  30          


Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr 
        35                  40                  45              


Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu Ala 
    50                  55                  60                  


His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro 
65                  70                  75                  80  


Gln Glu Val Glu Leu Val Asn Val Thr Glu Asn Phe Asn Met Trp Lys 
                85                  90                  95      


Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp 
            100                 105                 110         


Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu 
        115                 120                 125             


Asn Cys Thr Asp Leu Arg Asn Thr Thr Asn Thr Asn Asn Ser Thr Asp 
    130                 135                 140                 


Asn Asn Asn Ser Asn Ser Glu Gly Thr Ile Lys Gly Gly Glu Met Lys 
145                 150                 155                 160 


Asn Cys Ser Phe Asn Ile Ala Thr Ser Ile Gly Asp Lys Met Gln Lys 
                165                 170                 175     


Glu Tyr Ala Leu Leu Tyr Lys Leu Asp Ile Glu Pro Ile Asp Asn Asp 
            180                 185                 190         


Asn Thr Ser Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln 
        195                 200                 205             


Ala Cys Pro Lys Ile Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala 
    210                 215                 220                 


Pro Ala Gly Phe Ala Ile Leu Lys Cys Asn Asp Lys Lys Phe Ser Gly 
225                 230                 235                 240 


Lys Gly Ser Cys Lys Asn Val Ser Thr Val Gln Cys Thr His Gly Ile 
                245                 250                 255     


Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu 
            260                 265                 270         


Glu Glu Val Val Ile Arg Ser Glu Asn Phe Thr Asn Asn Ala Lys Thr 
        275                 280                 285             


Ile Ile Val Gln Leu Arg Glu Pro Val Lys Ile Asn Cys Ser Arg Pro 
    290                 295                 300                 


Asn Asn Asn Thr Arg Lys Ser Ile Pro Met Gly Pro Gly Arg Ala Phe 
305                 310                 315                 320 


Tyr Thr Thr Gly Gln Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn 
                325                 330                 335     


Ile Ser Lys Thr Asn Trp Thr Asn Ala Leu Lys Gln Val Val Glu Lys 
            340                 345                 350         


Leu Gly Glu Gln Phe Asn Lys Thr Lys Ile Val Phe Thr Asn Ser Ser 
        355                 360                 365             


Gly Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Ala Gly Glu 
    370                 375                 380                 


Phe Phe Tyr Cys Asn Thr Thr Gln Leu Phe Asp Ser Ile Trp Asn Ser 
385                 390                 395                 400 


Glu Asn Gly Thr Trp Asn Ile Thr Arg Gly Leu Asn Asn Thr Gly Arg 
                405                 410                 415     


Asn Asp Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Arg 
            420                 425                 430         


Trp Gln Glu Val Gly Lys Ala Met Tyr Ala Pro Pro Ile Lys Gly Asn 
        435                 440                 445             


Ile Ser Cys Ser Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly 
    450                 455                 460                 


Gly Lys Asp Asp Asn Ser Arg Asp Gly Asn Glu Thr Phe Arg Pro Gly 
465                 470                 475                 480 


Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys 
                485                 490                 495     


Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg 
            500                 505                 510         


Arg Val Val Gln Ser Glu Glu Ser Ala Val Gly Leu Gly Ala Met Phe 
        515                 520                 525             


Ile Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Val 
    530                 535                 540                 


Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
545                 550                 555                 560 


Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln 
                565                 570                 575     


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Ile Leu Ala Val 
            580                 585                 590         


Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser 
        595                 600                 605             


Gly Lys Leu Ile Cys Thr Thr Thr Val Pro Trp Asn Ala Ser Trp Ser 
    610                 615                 620                 


Lys Asn Lys Thr Leu Glu Ala Ile Trp Asn Asn Met Thr Trp Met Gln 
625                 630                 635                 640 


Trp Asp Lys Glu Ile Asp Asn Tyr Thr Ser Leu Ile Tyr Ser Leu Ile 
                645                 650                 655     


Glu Glu Ser Gln Ile Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu 
            660                 665                 670         


Leu Asp Lys Trp Ala Asn Leu Trp Asn Trp Phe Asp Ile Ser Asn Trp 
        675                 680                 685             


Leu Trp Tyr Ile Lys Lys Leu Met Lys Gln Ile Glu Asp Lys Ile Glu 
    690                 695                 700                 


Glu Ile Leu Ser Lys Ile Tyr His Ile Glu Asn Glu Ile Ala Arg Ile 
705                 710                 715                 720 


Lys Lys Leu Ile Gly Glu Ala Pro Gly Gly Ile Glu Gly Arg Lys Leu 
                725                 730                 735     


<210>  5
<211>  123
<212>  DNA
<213>  Artificial

<220>
<223>  GCN4 coiled coil

<400>  5
atgaagcaga tcgaggacaa gatcgaggag atcctgagca agatctacca catcgagaac     60

gagatcgcca ggatcaagaa gctgatcggc gaggcccctg gcggcatcga gggcaggaag    120

ctt                                                                  123


<210>  6
<211>  41
<212>  PRT
<213>  Artificial

<220>
<223>  GCN4 coiled coil

<400>  6

Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Leu Ser Lys Ile Tyr 
1               5                   10                  15      


His Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys Leu Ile Gly Glu Ala 
            20                  25                  30          


Pro Gly Gly Ile Glu Gly Arg Lys Leu 
        35                  40      


<210>  7
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  7
ctgtggtaca tcaagaagct ttaataatct agaggg                               36


<210>  8
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer

<400>  8
ccctctagat tattaaagct tcttgatgta ccacag                               36


<210>  9
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide linker

<400>  9

Gly Gly Ile Asp Gly Ile Leu Gln Ile Ser Ser Ser Gly Gly Ala 
1               5                   10                  15  


<210>  10
<211>  26
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide linker

<400>  10

Gly Gly Ile Asp Gly Ile Leu Gln Ile Ser Gly Ser Gly Ser Gly Gly 
1               5                   10                  15      


Ser Gly Gln Gly Ser Ser Ser Gly Gly Ala 
            20                  25      


<210>  11
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide linker

<400>  11

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
1               5                   10                  15  


<210>  12
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide linker

<400>  12

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly 
            20  


<210>  13
<211>  25
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide linker

<400>  13

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25  


