                         SEQUENCE LISTING

<110>  Cervin, Marguerite A.
       Kensch, Oliver
       Kettling, Ulrich
       Kim, Steve
       Leuthner, Birgitta
       Miasnikov, Andrei
       Ward, Michael
       Pellengahr, Klaus
 
<120>  Variant Buttiauxella sp. Phytases Having Altered Properties

<130>  30939WO2

<140>  Unassigned
<141>  2009-02-05

<150>  US 12/027,127
<151>  2008-02-06

<160>  14    

<170>  PatentIn version 3.4

<210>  1
<211>  446
<212>  PRT
<213>  Buttiauxella sp.

<400>  1
Met Thr Ile Ser Ala Phe Asn Arg Lys Lys Leu Thr Leu His Pro Gly 
1               5                   10                  15      
Leu Phe Val Ala Leu Ser Ala Ile Phe Ser Leu Gly Ser Thr Ala Tyr 
            20                  25                  30          
Ala Asn Asp Thr Pro Ala Ser Gly Tyr Gln Val Glu Lys Val Val Ile 
        35                  40                  45              
Leu Ser Arg His Gly Val Arg Ala Pro Thr Lys Met Thr Gln Thr Met 
    50                  55                  60                  
Arg Asp Val Thr Pro Asn Thr Trp Pro Glu Trp Pro Val Lys Leu Gly 
65                  70                  75                  80  
Tyr Ile Thr Pro Arg Gly Glu His Leu Ile Ser Leu Met Gly Gly Phe 
                85                  90                  95      
Tyr Arg Gln Lys Phe Gln Gln Gln Gly Ile Leu Ser Gln Gly Ser Cys 
            100                 105                 110         
Pro Thr Pro Asn Ser Ile Tyr Val Trp Ala Asp Val Asp Gln Arg Thr 
        115                 120                 125             
Leu Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Glu Cys His 
    130                 135                 140                 
Leu Thr Ile His His Gln Gln Asp Ile Lys Lys Ala Asp Pro Leu Phe 
145                 150                 155                 160 
His Pro Val Lys Ala Gly Thr Cys Ser Met Asp Lys Thr Gln Val Gln 
                165                 170                 175     
Gln Ala Val Glu Lys Glu Ala Gln Thr Pro Ile Asp Asn Leu Asn Gln 
            180                 185                 190         
His Tyr Ile Pro Phe Leu Ala Leu Met Asn Thr Thr Leu Asn Phe Ser 
        195                 200                 205             
Thr Ser Ala Trp Cys Gln Lys His Ser Ala Asp Lys Ser Cys Asp Leu 
    210                 215                 220                 
Gly Leu Ser Met Pro Ser Lys Leu Ser Ile Lys Asp Asn Gly Asn Lys 
225                 230                 235                 240 
Val Ala Leu Asp Gly Ala Ile Gly Leu Ser Ser Thr Leu Ala Glu Ile 
                245                 250                 255     
Phe Leu Leu Glu Tyr Ala Gln Gly Met Pro Gln Ala Ala Trp Gly Asn 
            260                 265                 270         
Ile His Ser Glu Gln Glu Trp Ala Ser Leu Leu Lys Leu His Asn Val 
        275                 280                 285             
Gln Phe Asp Leu Met Ala Arg Thr Pro Tyr Ile Ala Arg His Asn Gly 
    290                 295                 300                 
Thr Pro Leu Leu Gln Ala Ile Ser Asn Ala Leu Asn Pro Asn Ala Thr 
305                 310                 315                 320 
Glu Ser Lys Leu Pro Asp Ile Ser Pro Asp Asn Lys Ile Leu Phe Ile 
                325                 330                 335     
Ala Gly His Asp Thr Asn Ile Ala Asn Ile Ala Gly Met Leu Asn Met 
            340                 345                 350         
Arg Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Ala 
        355                 360                 365             
Leu Val Phe Glu Arg Leu Ala Asp Lys Ser Gly Lys Gln Tyr Val Ser 
    370                 375                 380                 
Val Ser Met Val Tyr Gln Thr Leu Glu Gln Leu Arg Ser Gln Thr Pro 
385                 390                 395                 400 
Leu Ser Leu Asn Gln Pro Ala Gly Ser Val Gln Leu Lys Ile Pro Gly 
                405                 410                 415     
Cys Asn Asp Gln Thr Ala Glu Gly Tyr Cys Pro Leu Ser Thr Phe Thr 
            420                 425                 430         
Arg Val Val Ser Gln Ser Val Glu Pro Gly Cys Gln Leu Gln 
        435                 440                 445     


<210>  2
<211>  413
<212>  PRT
<213>  Buttiauxella sp.

<400>  2
Asn Asp Thr Pro Ala Ser Gly Tyr Gln Val Glu Lys Val Val Ile Leu 
1               5                   10                  15      
Ser Arg His Gly Val Arg Ala Pro Thr Lys Met Thr Gln Thr Met Arg 
            20                  25                  30          
Asp Val Thr Pro Asn Thr Trp Pro Glu Trp Pro Val Lys Leu Gly Tyr 
        35                  40                  45              
Ile Thr Pro Arg Gly Glu His Leu Ile Ser Leu Met Gly Gly Phe Tyr 
    50                  55                  60                  
Arg Gln Lys Phe Gln Gln Gln Gly Ile Leu Ser Gln Gly Ser Cys Pro 
65                  70                  75                  80  
Thr Pro Asn Ser Ile Tyr Val Trp Ala Asp Val Asp Gln Arg Thr Leu 
                85                  90                  95      
Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Glu Cys His Leu 
            100                 105                 110         
Thr Ile His His Gln Gln Asp Ile Lys Lys Ala Asp Pro Leu Phe His 
        115                 120                 125             
Pro Val Lys Ala Gly Thr Cys Ser Met Asp Lys Thr Gln Val Gln Gln 
    130                 135                 140                 
Ala Val Glu Lys Glu Ala Gln Thr Pro Ile Asp Asn Leu Asn Gln His 
145                 150                 155                 160 
Tyr Ile Pro Phe Leu Ala Leu Met Asn Thr Thr Leu Asn Phe Ser Thr 
                165                 170                 175     
Ser Ala Trp Cys Gln Lys His Ser Ala Asp Lys Ser Cys Asp Leu Gly 
            180                 185                 190         
Leu Ser Met Pro Ser Lys Leu Ser Ile Lys Asp Asn Gly Asn Lys Val 
        195                 200                 205             
Ala Leu Asp Gly Ala Ile Gly Leu Ser Ser Thr Leu Ala Glu Ile Phe 
    210                 215                 220                 
Leu Leu Glu Tyr Ala Gln Gly Met Pro Gln Ala Ala Trp Gly Asn Ile 
225                 230                 235                 240 
His Ser Glu Gln Glu Trp Ala Ser Leu Leu Lys Leu His Asn Val Gln 
                245                 250                 255     
Phe Asp Leu Met Ala Arg Thr Pro Tyr Ile Ala Arg His Asn Gly Thr 
            260                 265                 270         
Pro Leu Leu Gln Ala Ile Ser Asn Ala Leu Asn Pro Asn Ala Thr Glu 
        275                 280                 285             
Ser Lys Leu Pro Asp Ile Ser Pro Asp Asn Lys Ile Leu Phe Ile Ala 
    290                 295                 300                 
Gly His Asp Thr Asn Ile Ala Asn Ile Ala Gly Met Leu Asn Met Arg 
305                 310                 315                 320 
Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Ala Leu 
                325                 330                 335     
Val Phe Glu Arg Leu Ala Asp Lys Ser Gly Lys Gln Tyr Val Ser Val 
            340                 345                 350         
Ser Met Val Tyr Gln Thr Leu Glu Gln Leu Arg Ser Gln Thr Pro Leu 
        355                 360                 365             
Ser Leu Asn Gln Pro Ala Gly Ser Val Gln Leu Lys Ile Pro Gly Cys 
    370                 375                 380                 
Asn Asp Gln Thr Ala Glu Gly Tyr Cys Pro Leu Ser Thr Phe Thr Arg 
385                 390                 395                 400 
Val Val Ser Gln Ser Val Glu Pro Gly Cys Gln Leu Gln 
                405                 410             

<210>  3
<211>  413
<212>  PRT
<213>  Buttiauxella sp.

<400>  3
Asn Asp Thr Pro Ala Ser Gly Tyr Gln Val Glu Lys Val Val Ile Leu 
1               5                   10                  15      
Ser Arg His Gly Val Arg Ala Pro Thr Lys Met Thr Gln Thr Met Arg 
            20                  25                  30          
Asp Val Thr Pro Asn Thr Trp Pro Glu Trp Pro Val Lys Leu Gly Tyr 
        35                  40                  45              
Ile Thr Pro Arg Gly Glu His Leu Ile Ser Leu Met Gly Gly Phe Tyr 
    50                  55                  60                  
Arg Gln Lys Phe Gln Gln Gln Gly Ile Leu Ser Gln Gly Ser Cys Pro 
65                  70                  75                  80  
Thr Pro Asn Ser Ile Tyr Val Trp Thr Asp Val Ala Gln Arg Thr Leu 
                85                  90                  95      
Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Gln Cys Gly Leu 
            100                 105                 110         
Thr Ile His His Gln Gln Asn Leu Glu Lys Ala Asp Pro Leu Phe His 
        115                 120                 125             
Pro Val Lys Ala Gly Ile Cys Ser Met Asp Lys Thr Gln Val Gln Gln 
    130                 135                 140                 
Ala Val Glu Lys Glu Ala Gln Thr Pro Ile Asp Asn Leu Asn Gln His 
145                 150                 155                 160 
Tyr Ile Pro Ser Leu Ala Leu Met Asn Thr Thr Leu Asn Phe Ser Lys 
                165                 170                 175     
Ser Pro Trp Cys Gln Lys His Ser Ala Asp Lys Ser Cys Asp Leu Gly 
            180                 185                 190         
Leu Ser Met Pro Ser Lys Leu Ser Ile Lys Asp Asn Gly Asn Glu Val 
        195                 200                 205             
Ser Leu Asp Gly Ala Ile Gly Leu Ser Ser Thr Leu Ala Glu Ile Phe 
    210                 215                 220                 
Leu Leu Glu Tyr Ala Gln Gly Met Pro Gln Ala Ala Trp Gly Asn Ile 
225                 230                 235                 240 
His Ser Glu Gln Glu Trp Ala Leu Leu Leu Lys Leu His Asn Val Tyr 
                245                 250                 255     
Phe Asp Leu Met Glu Arg Thr Pro Tyr Ile Ala Arg His Lys Gly Thr 
            260                 265                 270         
Pro Leu Leu Gln Ala Ile Ser Asn Ala Leu Asn Pro Asn Ala Thr Glu 
        275                 280                 285             
Ser Lys Leu Pro Asp Ile Ser Pro Asp Asn Lys Ile Leu Phe Ile Ala 
    290                 295                 300                 
Gly His Asp Thr Asn Ile Ala Asn Ile Ala Gly Met Leu Asn Met Arg 
305                 310                 315                 320 
Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Ala Leu 
                325                 330                 335     
Val Phe Glu Arg Leu Ala Asp Lys Ser Gly Lys Gln Tyr Val Ser Val 
            340                 345                 350         
Ser Met Val Tyr Gln Thr Leu Glu Gln Leu Arg Ser Gln Thr Pro Leu 
        355                 360                 365             
Ser Leu Asn Gln Pro Ala Gly Ser Val Gln Leu Lys Ile Pro Gly Cys 
    370                 375                 380                 
Asn Asp Gln Thr Ala Glu Gly Tyr Cys Pro Leu Ser Thr Phe Thr Arg 
385                 390                 395                 400 
Val Val Ser Gln Ser Val Glu Pro Gly Cys Gln Leu Gln 
                405                 410             

<210>  4
<211>  419
<212>  PRT
<213>  Artificial

<220>
<223>  modified protein

<400>  4
His His His His His His Asn Asp Thr Pro Ala Ser Gly Tyr Gln Val 
1               5                   10                  15      
Glu Lys Val Val Ile Leu Ser Arg His Gly Val Arg Ala Pro Thr Lys 
            20                  25                  30          
Met Thr Gln Thr Met Arg Asp Val Thr Pro Asn Thr Trp Pro Glu Trp 
        35                  40                  45              
Pro Val Lys Leu Gly Tyr Ile Thr Pro Arg Gly Glu His Leu Ile Ser 
    50                  55                  60                  
Leu Met Gly Gly Phe Tyr Arg Gln Lys Phe Gln Gln Gln Gly Ile Leu 
65                  70                  75                  80  
Ser Gln Gly Ser Cys Pro Thr Pro Asn Ser Ile Tyr Val Trp Thr Asp 
                85                  90                  95      
Val Asp Gln Arg Thr Leu Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu 
            100                 105                 110         
Ala Pro Gln Cys Gly Leu Thr Ile His His Gln Gln Asn Leu Glu Lys 
        115                 120                 125             
Ala Asp Pro Leu Phe His Pro Val Lys Ala Gly Ile Cys Ser Met Asp 
    130                 135                 140                 
Lys Thr Gln Val Gln Gln Ala Val Glu Lys Glu Ala Gln Thr Pro Ile 
145                 150                 155                 160 
Asp Asn Leu Asn Gln His Tyr Ile Pro Ser Leu Ala Leu Met Asn Thr 
                165                 170                 175     
Thr Leu Asn Phe Ser Lys Ser Pro Trp Cys Gln Lys His Ser Ala Asp 
            180                 185                 190         
Lys Ser Cys Asp Leu Gly Leu Ser Met Pro Ser Lys Leu Ser Ile Lys 
        195                 200                 205             
Asp Asn Gly Asn Glu Val Ser Leu Asp Gly Ala Ile Gly Leu Ser Ser 
    210                 215                 220                 
Thr Leu Ala Glu Ile Phe Leu Leu Glu Tyr Ala Gln Gly Met Pro Gln 
225                 230                 235                 240 
Ala Ala Trp Gly Asn Ile His Ser Glu Gln Glu Trp Ala Leu Leu Leu 
                245                 250                 255     
Lys Leu His Asn Val Tyr Phe Asp Leu Met Glu Arg Thr Pro Tyr Ile 
            260                 265                 270         
Ala Arg His Lys Gly Thr Pro Leu Leu Gln Ala Ile Ser Asn Ala Leu 
        275                 280                 285             
Asn Pro Asn Ala Thr Glu Ser Lys Leu Pro Asp Ile Ser Pro Asp Asn 
    290                 295                 300                 
Lys Ile Leu Phe Ile Ala Gly His Asp Thr Asn Ile Ala Asn Ile Ala 
305                 310                 315                 320 
Gly Met Leu Asn Met Arg Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr 
                325                 330                 335     
Pro Pro Gly Gly Ala Leu Val Phe Glu Arg Leu Ala Asp Lys Ser Gly 
            340                 345                 350         
Lys Gln Tyr Val Ser Val Ser Met Val Tyr Gln Thr Leu Glu Gln Leu 
        355                 360                 365             
Arg Ser Gln Thr Pro Leu Ser Leu Asn Gln Pro Ala Gly Ser Val Gln 
    370                 375                 380                 
Leu Lys Ile Pro Gly Cys Asn Asp Gln Thr Ala Glu Gly Tyr Cys Pro 
385                 390                 395                 400 
Leu Ser Thr Phe Thr Arg Val Val Ser Gln Ser Val Glu Pro Gly Cys 
                405                 410                 415     
Gln Leu Gln 
            

<210>  5
<211>  1534
<212>  DNA
<213>  Buttiauxella sp.

<400>  5
tttcacatag caaacaacaa cgagacgaac tcgacgttac cgctttgctt ctggagtata     60
tttatcagac tcaaacaccc caaagaaaag aggctgtaaa tgacgatctc tgcgtttaac    120
cgcaaaaaac tgacgcttca ccctggtctg ttcgtagcac tgagcgccat attttcatta    180
ggctctacgg cctatgccaa cgacactccc gcttcaggct accaggttga gaaagtggta    240
atactcagcc gccacggggt gcgagcacca accaaaatga cacagaccat gcgcgacgta    300
acacctaata cctggcccga atggccagta aaattgggtt atatcacgcc acgcggtgag    360
catctgatta gcctgatggg cgggttttat cgccagaagt ttcaacaaca gggcatttta    420
tcgcagggca gttgccccac accaaactca atttatgtct gggcagacgt tgatcagcgc    480
acgcttaaaa ctggcgaagc tttcctggca gggcttgctc cggaatgtca tttaactatt    540
caccaccagc aggacatcaa aaaagccgat ccgctgttcc atccggtgaa agcgggcacc    600
tgttcaatgg ataaaactca ggtccaacag gccgttgaaa aagaagctca aacccccatt    660
gataatctga atcagcacta tattcccttt ctggccttga tgaatacgac cctcaacttt    720
tcgacgtcgg cctggtgtca gaaacacagc gcggataaaa gctgtgattt agggctatcc    780
atgccgagca agctgtcgat aaaagataat ggcaacaaag tcgctctcga cggggccatt    840
ggcctttcgt ctacgcttgc tgaaattttc ctgctggaat atgcgcaagg gatgccgcaa    900
gcggcgtggg ggaatattca ttcagagcaa gagtgggcgt cgctactgaa actgcataac    960
gtccagtttg atttgatggc acgcacgcct tatatcgcca gacataacgg cacgccttta   1020
ttgcaggcca tcagcaacgc gctgaacccg aatgccaccg aaagcaaact gcctgatatc   1080
tcacctgaca ataagatcct gtttattgcc ggacacgata ccaatattgc caatatcgca   1140
ggcatgctca acatgcgctg gacgctacct gggcaacccg ataacacccc tccgggcggc   1200
gctttagtct ttgagcgttt ggccgataag tcagggaaac aatatgttag cgtgagcatg   1260
gtgtatcaga ctctcgagca gttgcgctcc caaacaccac ttagccttaa tcaacctgcg   1320
ggaagcgtac agctaaaaat tcctggctgt aacgatcaga cggctgaagg atactgcccg   1380
ctgtcgacgt tcactcgcgt ggttagccaa agcgtggaac caggctgcca gctacagtaa   1440
atatcagaca aaaaaaatgc cgctcgcgat taagcgaacg gcattacttc ctagcttccc   1500
agctcggatt agcatggcga gagccgaaaa actt                               1534

<210>  6
<211>  413
<212>  PRT
<213>  Buttiauxella sp.

<400>  6
Asn Asp Thr Pro Ala Ser Gly Tyr Gln Val Glu Lys Val Val Ile Leu 
1               5                   10                  15      
Ser Arg His Gly Val Arg Ala Pro Thr Lys Met Thr Gln Thr Met Arg 
            20                  25                  30          
Asp Val Thr Pro Asn Thr Trp Pro Glu Trp Pro Val Lys Leu Gly Tyr 
        35                  40                  45              
Ile Thr Pro Arg Gly Glu His Leu Ile Ser Leu Met Gly Gly Phe Tyr 
    50                  55                  60                  
Arg Gln Lys Phe Gln Gln Gln Gly Ile Leu Ser Gln Gly Ser Cys Pro 
65                  70                  75                  80  
Thr Pro Asn Ser Ile Tyr Val Trp Thr Asp Val Asp Gln Arg Thr Leu 
                85                  90                  95      
Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Gln Cys Gly Leu 
            100                 105                 110         
Thr Ile His His Gln Gln Asn Leu Glu Lys Ala Asp Pro Leu Phe His 
        115                 120                 125             
Pro Val Lys Ala Gly Ile Cys Ser Met Asp Lys Thr Gln Val Gln Gln 
    130                 135                 140                 
Ala Val Glu Lys Glu Ala Gln Thr Pro Ile Asp Asn Leu Asn Gln His 
145                 150                 155                 160 
Tyr Ile Pro Ser Leu Ala Leu Met Asn Thr Thr Leu Asn Phe Ser Lys 
                165                 170                 175     
Ser Pro Trp Cys Gln Lys His Ser Ala Asp Lys Ser Cys Asp Leu Gly 
            180                 185                 190         
Leu Ser Met Pro Ser Lys Leu Ser Ile Lys Asp Asn Gly Asn Glu Val 
        195                 200                 205             
Ser Leu Asp Gly Ala Ile Gly Leu Ser Ser Thr Leu Ala Glu Ile Phe 
    210                 215                 220                 
Leu Leu Glu Tyr Ala Gln Gly Met Pro Gln Ala Ala Trp Gly Asn Ile 
225                 230                 235                 240 
His Ser Glu Gln Glu Trp Ala Leu Leu Leu Lys Leu His Asn Val Tyr 
                245                 250                 255     
Phe Asp Leu Met Glu Arg Thr Pro Tyr Ile Ala Arg His Lys Gly Thr 
            260                 265                 270         
Pro Leu Leu Gln Ala Ile Ser Asn Ala Leu Asn Pro Asn Ala Thr Glu 
        275                 280                 285             
Ser Lys Leu Pro Asp Ile Ser Pro Asp Asn Lys Ile Leu Phe Ile Ala 
    290                 295                 300                 
Gly His Asp Thr Asn Ile Ala Asn Ile Ala Gly Met Leu Asn Met Arg 
305                 310                 315                 320 
Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Ala Leu 
                325                 330                 335     
Val Phe Glu Arg Leu Ala Asp Lys Ser Gly Lys Gln Tyr Val Ser Val 
            340                 345                 350         
Ser Met Val Tyr Gln Thr Leu Glu Gln Leu Arg Ser Gln Thr Pro Leu 
        355                 360                 365             
Ser Leu Asn Gln Pro Ala Gly Ser Val Gln Leu Lys Ile Pro Gly Cys 
    370                 375                 380                 
Asn Asp Gln Thr Ala Glu Gly Tyr Cys Pro Leu Ser Thr Phe Thr Arg 
385                 390                 395                 400 
Val Val Ser Gln Ser Val Glu Pro Gly Cys Gln Leu Gln 
                405                 410             

<210>  7
<211>  1274
<212>  DNA
<213>  Artificial

<220>
<223>  modified protein

<400>  7
actagtgtcg ccgtggagaa gcgcaacgac acccccgcca gcggctacca ggtcgagaag     60
gtcgtcatcc tcagccgcca cggcgtccgc gcccctacca agatgaccca gaccatgcgc    120
gacgtcaccc ccaacacctg gcccgagtgg cccgtcaagc tcggctacat cacccctcgc    180
ggcgagcacc tcatcagcct catgggcggc ttctaccgcc agaagttcca gcagcagggc    240
atcctcagcc agggctcgtg ccccaccccc aacagcatct acgtctggac cgacgtcgcc    300
cagcgcaccc tcaagaccgg cgaggccttc ctcgccggcc tcgcccccca gtgcggcctc    360
accatccacc accagcagaa cctcgagaag gccgaccccc tcttccaccc cgtcaaggcc    420
ggcatctgca gcatggacaa gacccaggtc cagcaggccg tcgagaagga ggcccagacc    480
cccatcgaca acctcaacca gcactacatc cccagcctcg ccctcatgaa caccaccctc    540
aacttcagca agagcccctg gtgccagaag cacagcgccg acaagagctg cgacctcggc    600
ctcagcatgc ccagcaagct cagcatcaag gacaacggca acgaggtctc cctcgacggc    660
gctatcggcc tcagctccac cctcgccgag atcttcctcc tcgagtacgc ccagggcatg    720
cctcaggccg cctggggcaa catccacagc gagcaggagt gggccctcct cctcaagctc    780
cacaacgtct acttcgacct catggagcgc accccctaca tcgcccgcca caagggcacc    840
cccctcctcc aggccatcag caacgccctc aaccccaacg ccaccgagag caagctcccc    900
gacatcagcc ccgacaacaa gatcctcttc atcgccggcc acgacaccaa catcgccaac    960
atcgccggca tgctcaacat gcgctggacc ctccccggcc agcccgacaa caccccccct   1020
ggcggcgctc tcgtctttga gcgcctcgcc gacaagtccg gcaagcagta cgtcagcgtc   1080
agcatggtct accagaccct cgagcagctc cgcagccaga cccccctcag cctcaaccag   1140
cctgccggca gcgtccagct caagatcccc ggctgcaacg accagaccgc cgagggctac   1200
tgccccctca gcaccttcac ccgcgtcgtc agccagagcg tcgagcccgg ctgccagctc   1260
cagtaaggcg cgcc                                                     1274

<210>  8
<211>  1256
<212>  DNA
<213>  Buttiauxella sp.

<400>  8
actagtaacg acacccccgc cagcggctac caggtcgaga aggtcgtcat cctcagccgc     60
cacggagtcc gcgcccccac caagatgacc cagaccatgc gcgacgtcac ccccaacacc    120
tggcccgagt ggcccgtcaa gctcggctac atcacccccc gcggcgagca cctcatcagc    180
ctcatgggcg gcttctaccg ccagaagttc cagcagcagg gcatcctcag ccagggctcg    240
tgtcccaccc ccaacagcat ctatgtctgg gccgacgtcg accagcgcac cctcaagacc    300
ggcgaggcct tcctcgccgg cctcgccccc cagtgcggcc tcaccatcca ccaccagcag    360
aacctcgaga aggccgaccc cctcttccac cccgtcaagg ccggcacctg cagcatggac    420
aagacccagg tccagcaggc cgtcgagaag gaggcccaga cccccatcga caacctcaac    480
cagcactaca tccccttcct cgccctcatg aacaccaccc tcaacttcag caccagcgcc    540
tggtgccaga agcacagcgc cgacaagagc tgcgacctcg gcctcagcat gcccagcaag    600
ctcagcatca aggacaacgg caacaaggtc gccctcgacg gcgctatcgg cctcagctcc    660
accctcgccg agatcttcct cctcgagtac gcccagggca tgcctcaggc tgcctggggc    720
aacatccaca gcgagcagga gtgggccagc ctcctcaagc tccacaacgt ccagttcgac    780
ctcatggccc gcacccccta catcgcccgc cacaacggca cccccctcct ccaggccatc    840
agcaacgccc tcaaccccaa cgccaccgag agcaagctcc ccgacatcag ccccgacaac    900
aagatcctct tcatcgccgg ccacgacacc aacatcgcca acatcgccgg catgctcaac    960
atgcgctgga ccctccccgg ccagcccgac aacacccccc ccggcggcgc tctcgtcttt   1020
gagcgcctcg ccgacaagtc cggcaagcaa tatgtctctg tcagcatggt ctaccagacc   1080
ctcgagcagc tccgcagcca gacccccctc agcctcaacc agcccgccgg cagcgtccag   1140
ctcaagatcc ccggctgcaa cgaccagacc gccgagggct actgccccct cagcaccttc   1200
acccgcgtcg tcagccagag cgtcgagccc ggctgccagc tccagtaagg cgcgcc       1256

<210>  9
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  9
cactactagt gtcgctgtgg agaagcgcaa cgacaccccc gccag                     45

<210>  10
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic primer

<400>  10

Val Ala Val Glu Lys Arg 
1               5       

<210>  11
<211>  82
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  11
caccatgcag accttcggtg cttttctcgt ttccttcctc gccgccagcg gcctggccgc     60
ggccaacgac acccccgcca gc                                              82

<210>  12
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  12
ccttactgga gctggcag                                                   18

<210>  13
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic primer

<400>  13
gagttcggcg cgccttactg ga                                              22

<210>  14
<211>  1242
<212>  DNA
<213>  Artificial

<220>
<223>  modified protein

<400>  14
aacgacaccc ccgccagcgg ctaccaggtc gagaaggtcg tcatcctcag ccgccacggc     60
gtccgcgccc ctaccaagat gacccagacc atgcgcgacg tcacccccaa cacctggccc    120
gagtggcccg tcaagctcgg ctacatcacc cctcgcggcg agcacctcat cagcctcatg    180
ggcggcttct accgccagaa gttccagcag cagggcatcc tcagccaggg ctcgtgcccc    240
acccccaaca gcatctacgt ctggactgac gtcgcccagc gcaccctcaa gaccggcgag    300
gccttcctcg ccggcctcgc cccccagtgc ggcctcacca tccaccacca gcagaacctc    360
gagaaggccg accccctctt ccaccccgtc aaggccggca tctgcagcat ggacaagacc    420
caggtccagc aggccgtcga gaaggaggcc cagaccccca tcgacaacct caaccagcac    480
tacatcccca gcctcgccct catgaacacc accctcaact tcagcaagag cccctggtgc    540
cagaagcaca gcgccgacaa gagctgcgac ctcggcctca gcatgcccag caagctcagc    600
atcaaggaca acggcaacga ggtctccctc gacggcgcta tcggcctcag ctccaccctc    660
gccgagatct tcctcctcga gtacgcccag ggcatgcctc aggccgcctg gggcaacatc    720
cacagcgagc aggagtgggc cctcctcctc aagctccaca acgtctactt cgacctcatg    780
gagcgcaccc cctacatcgc ccgccacaag ggcacccccc tcctccaggc catcagcaac    840
gccctcaacc ccaacgccac cgagagcaag ctccccgaca tcagccccga caacaagatc    900
ctcttcatcg ccggccacga caccaacatc gccaacatcg ccggcatgct caacatgcgc    960
tggaccctcc ccggccagcc cgacaacacc ccccctggcg gcgctctcgt ctttgagcgc   1020
ctcgccgaca agtccggcaa gcagtacgtc agcgtcagca tggtctacca gaccctcgag   1080
cagctccgca gccagacccc cctcagcctc aaccagcctg ccggcagcgt ccagctcaag   1140
atccccggct gcaacgacca gaccgccgag ggctactgcc ccctcagcac cttcacccgc   1200
gtcgtcagcc agagcgtcga gcccggctgc cagctccagt aa                      1242
