                         SEQUENCE LISTING

<110>  Vellard, et al.
 
<120>  MANUFACTURE OF ACTIVE HIGHLY PHOSPHORYLATED HUMAN LYSOSOMAL 
       SULFATASE ENZYMES AND USES THEREOF

<130>  30610/43643

<150>  61/022,179
<151>  2008-01-18

<150>  61/099,373
<151>  2008-09-23

<150>  61/110,246
<151>  2008-10-31

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  1125                             
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human Sulfatase Modifying Factor 1 (SUMF1) Polynucleotide 
       Sequence

<400>  1
atggctgcgc ccgcactagg gctggtgtgt ggacgttgcc ctgagctggg tctcgtcctc       60

ttgctgctgc tgctctcgct gctgtgtgga gcggcaggga gccaggaggc cgggaccggt      120

gcgggcgcgg ggtcccttgc gggttcttgc ggctgcggca cgccccagcg gcctggcgcc      180

catggcagtt cggcagccgc tcaccgatac tcgcgggagg ctaacgctcc gggccccgta      240

cccggagagc ggcaactcgc gcactcaaag atggtcccca tccctgctgg agtatttaca      300

atgggcacag atgatcctca gataaagcag gatggggaag cacctgcgag gagagttact      360

attgatgcct tttacatgga tgcctatgaa gtcagtaata ctgaatttga gaagtttgtg      420

aactcaactg gctatttgac agaggctgag aagtttggcg actcctttgt ctttgaaggc      480

atgttgagtg agcaagtgaa gaccaatatt caacaggcag ttgcagctgc tccctggtgg      540

ttacctgtga aaggcgctaa ctggagacac ccagaagggc ctgactctac tattctgcac      600

aggccggatc atccagttct ccatgtgtcc tggaatgatg cggttgccta ctgcacttgg      660

gcagggaagc ggctgcccac ggaagctgag tgggaataca gctgtcgagg aggcctgcat      720

aatagacttt tcccctgggg caacaaactg cagcccaaag gccagcatta tgccaacatt      780

tggcagggcg agtttccggt gaccaacact ggtgaggatg gcttccaagg aactgcgcct      840

gttgatgcct tccctcccaa tggttatggc ttatacaaca tagtggggaa cgcatgggaa      900

tggacttcag actggtggac tgttcatcat tctgttgaag aaacgcttaa cccaaaaggt      960

cccccttctg ggaaagaccg agtgaagaaa ggtggatcct acatgtgcca taggtcttat     1020

tgttacaggt atcgctgtgc tgctcggagc cagaacacac ctgatagctc tgcttcgaat     1080

ctgggattcc gctgtgcagc cgaccgcctg cccaccatgg actga                     1125


<210>  2
<211>  374
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Human Sulfatase Modifying Factor 1 (SUMF1) Polypeptide Sequence

<400>  2

Met Ala Ala Pro Ala Leu Gly Leu Val Cys Gly Arg Cys Pro Glu Leu 
1               5                   10                  15      


Gly Leu Val Leu Leu Leu Leu Leu Leu Ser Leu Leu Cys Gly Ala Ala 
            20                  25                  30          


Gly Ser Gln Glu Ala Gly Thr Gly Ala Gly Ala Gly Ser Leu Ala Gly 
        35                  40                  45              


Ser Cys Gly Cys Gly Thr Pro Gln Arg Pro Gly Ala His Gly Ser Ser 
    50                  55                  60                  


Ala Ala Ala His Arg Tyr Ser Arg Glu Ala Asn Ala Pro Gly Pro Val 
65                  70                  75                  80  


Pro Gly Glu Arg Gln Leu Ala His Ser Lys Met Val Pro Ile Pro Ala 
                85                  90                  95      


Gly Val Phe Thr Met Gly Thr Asp Asp Pro Gln Ile Lys Gln Asp Gly 
            100                 105                 110         


Glu Ala Pro Ala Arg Arg Val Thr Ile Asp Ala Phe Tyr Met Asp Ala 
        115                 120                 125             


Tyr Glu Val Ser Asn Thr Glu Phe Glu Lys Phe Val Asn Ser Thr Gly 
    130                 135                 140                 


Tyr Leu Thr Glu Ala Glu Lys Phe Gly Asp Ser Phe Val Phe Glu Gly 
145                 150                 155                 160 


Met Leu Ser Glu Gln Val Lys Thr Asn Ile Gln Gln Ala Val Ala Ala 
                165                 170                 175     


Ala Pro Trp Trp Leu Pro Val Lys Gly Ala Asn Trp Arg His Pro Glu 
            180                 185                 190         


Gly Pro Asp Ser Thr Ile Leu His Arg Pro Asp His Pro Val Leu His 
        195                 200                 205             


Val Ser Trp Asn Asp Ala Val Ala Tyr Cys Thr Trp Ala Gly Lys Arg 
    210                 215                 220                 


Leu Pro Thr Glu Ala Glu Trp Glu Tyr Ser Cys Arg Gly Gly Leu His 
225                 230                 235                 240 


Asn Arg Leu Phe Pro Trp Gly Asn Lys Leu Gln Pro Lys Gly Gln His 
                245                 250                 255     


Tyr Ala Asn Ile Trp Gln Gly Glu Phe Pro Val Thr Asn Thr Gly Glu 
            260                 265                 270         


Asp Gly Phe Gln Gly Thr Ala Pro Val Asp Ala Phe Pro Pro Asn Gly 
        275                 280                 285             


Tyr Gly Leu Tyr Asn Ile Val Gly Asn Ala Trp Glu Trp Thr Ser Asp 
    290                 295                 300                 


Trp Trp Thr Val His His Ser Val Glu Glu Thr Leu Asn Pro Lys Gly 
305                 310                 315                 320 


Pro Pro Ser Gly Lys Asp Arg Val Lys Lys Gly Gly Ser Tyr Met Cys 
                325                 330                 335     


His Arg Ser Tyr Cys Tyr Arg Tyr Arg Cys Ala Ala Arg Ser Gln Asn 
            340                 345                 350         


Thr Pro Asp Ser Ser Ala Ser Asn Leu Gly Phe Arg Cys Ala Ala Asp 
        355                 360                 365             


Arg Leu Pro Thr Met Asp 
    370                 


<210>  3
<211>  1569
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human N-Acetylgalactosamine-6_Sulfatase (GALNS) Polynucleotide 
       Sequence

<400>  3
atggcggcgg ttgtcgcggc gacgaggtgg tggcagctgt tgctggtgct cagcgccgcg       60

gggatggggg cctcgggcgc cccgcagccc cccaacatcc tgctcctgct catggacgac      120

atgggatggg gtgacctcgg ggtgtatgga gagccctcca gagagacccc gaatttggac      180

cggatggctg cagaagggct gcttttccca aacttctatt ctgccaaccc tctgtgctcg      240

ccatcgaggg cggcactgct cacaggacgg ctacccatcc gcaatggctt ctacaccacc      300

aacgcccatg ccagaaacgc ctacacaccg caggagattg tgggcggcat cccagactcg      360

gagcagctcc tgccggagct tctgaagaag gccggctacg tcagcaagat tgtcggcaag      420

tggcatctgg gtcacaggcc ccagttccac cccctgaagc acggatttga tgagtggttt      480

ggatccccca actgccactt tggaccttat gacaacaagg ccaggcccaa catccctgtg      540

tacagggact gggagatggt tggcagatat tatgaagaat ttcctattaa tctgaagacg      600

ggggaagcca acctcaccca gatctacctg caggaagccc tggacttcat taagagacag      660

gcacggcacc accccttttt cctctactgg gctgtcgacg ccacgcacgc acccgtctat      720

gcctccaaac ccttcttggg caccagtcag cgagggcggt atggagacgc cgtccgggag      780

attgatgaca gcattgggaa gatactggag ctcctccaag acctgcacgt cgcggacaac      840

accttcgtct tcttcacgtc ggacaacggc gctgccctca tttccgcccc cgaacaaggt      900

ggcagcaacg gcccctttct gtgtgggaag cagaccacgt ttgaaggagg gatgagggag      960

cctgccctcg catggtggcc agggcacgtc actgcaggcc aggtgagcca ccagctgggc     1020

agcatcatgg acctcttcac caccagcctg gcccttgcgg gcctgacgcc gcccagcgac     1080

agggccattg atggcctcaa cctcctcccc accctcctgc agggccggct gatggacagg     1140

cctatcttct attaccgtgg cgacacgctg atggcggcca ccctcgggca gcacaaggct     1200

cacttctgga cctggaccaa ctcctgggag aacttcagac agggcattga tttctgccct     1260

gggcagaacg tttcaggggt cacaactcac aatctggaag accacacgaa gctgcccctg     1320

atcttccacc tgggacggga cccaggggag aggttccccc tcagctttgc cagcgccgag     1380

taccaggagg ccctcagcag gatcacctcg gtcgtccagc agcaccagga ggccttggtc     1440

cccgcgcagc cccagctcaa cgtgtgcaac tgggcggtca tgaactgggc acctccgggc     1500

tgtgaaaagt tagggaagtg tctgacacct ccagaatcca ttcccaagaa gtgcctctgg     1560

tcccactag                                                             1569


<210>  4
<211>  522
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Human N-acetylgalactosamine-6-Sulfatase (GALNS) Polypeptide 
       Sequence

<400>  4

Met Ala Ala Val Val Ala Ala Thr Arg Trp Trp Gln Leu Leu Leu Val 
1               5                   10                  15      


Leu Ser Ala Ala Gly Met Gly Ala Ser Gly Ala Pro Gln Pro Pro Asn 
            20                  25                  30          


Ile Leu Leu Leu Leu Met Asp Asp Met Gly Trp Gly Asp Leu Gly Val 
        35                  40                  45              


Tyr Gly Glu Pro Ser Arg Glu Thr Pro Asn Leu Asp Arg Met Ala Ala 
    50                  55                  60                  


Glu Gly Leu Leu Phe Pro Asn Phe Tyr Ser Ala Asn Pro Leu Cys Ser 
65                  70                  75                  80  


Pro Ser Arg Ala Ala Leu Leu Thr Gly Arg Leu Pro Ile Arg Asn Gly 
                85                  90                  95      


Phe Tyr Thr Thr Asn Ala His Ala Arg Asn Ala Tyr Thr Pro Gln Glu 
            100                 105                 110         


Ile Val Gly Gly Ile Pro Asp Ser Glu Gln Leu Leu Pro Glu Leu Leu 
        115                 120                 125             


Lys Lys Ala Gly Tyr Val Ser Lys Ile Val Gly Lys Trp His Leu Gly 
    130                 135                 140                 


His Arg Pro Gln Phe His Pro Leu Lys His Gly Phe Asp Glu Trp Phe 
145                 150                 155                 160 


Gly Ser Pro Asn Cys His Phe Gly Pro Tyr Asp Asn Lys Ala Arg Pro 
                165                 170                 175     


Asn Ile Pro Val Tyr Arg Asp Trp Glu Met Val Gly Arg Tyr Tyr Glu 
            180                 185                 190         


Glu Phe Pro Ile Asn Leu Lys Thr Gly Glu Ala Asn Leu Thr Gln Ile 
        195                 200                 205             


Tyr Leu Gln Glu Ala Leu Asp Phe Ile Lys Arg Gln Ala Arg His His 
    210                 215                 220                 


Pro Phe Phe Leu Tyr Trp Ala Val Asp Ala Thr His Ala Pro Val Tyr 
225                 230                 235                 240 


Ala Ser Lys Pro Phe Leu Gly Thr Ser Gln Arg Gly Arg Tyr Gly Asp 
                245                 250                 255     


Ala Val Arg Glu Ile Asp Asp Ser Ile Gly Lys Ile Leu Glu Leu Leu 
            260                 265                 270         


Gln Asp Leu His Val Ala Asp Asn Thr Phe Val Phe Phe Thr Ser Asp 
        275                 280                 285             


Asn Gly Ala Ala Leu Ile Ser Ala Pro Glu Gln Gly Gly Ser Asn Gly 
    290                 295                 300                 


Pro Phe Leu Cys Gly Lys Gln Thr Thr Phe Glu Gly Gly Met Arg Glu 
305                 310                 315                 320 


Pro Ala Leu Ala Trp Trp Pro Gly His Val Thr Ala Gly Gln Val Ser 
                325                 330                 335     


His Gln Leu Gly Ser Ile Met Asp Leu Phe Thr Thr Ser Leu Ala Leu 
            340                 345                 350         


Ala Gly Leu Thr Pro Pro Ser Asp Arg Ala Ile Asp Gly Leu Asn Leu 
        355                 360                 365             


Leu Pro Thr Leu Leu Gln Gly Arg Leu Met Asp Arg Pro Ile Phe Tyr 
    370                 375                 380                 


Tyr Arg Gly Asp Thr Leu Met Ala Ala Thr Leu Gly Gln His Lys Ala 
385                 390                 395                 400 


His Phe Trp Thr Trp Thr Asn Ser Trp Glu Asn Phe Arg Gln Gly Ile 
                405                 410                 415     


Asp Phe Cys Pro Gly Gln Asn Val Ser Gly Val Thr Thr His Asn Leu 
            420                 425                 430         


Glu Asp His Thr Lys Leu Pro Leu Ile Phe His Leu Gly Arg Asp Pro 
        435                 440                 445             


Gly Glu Arg Phe Pro Leu Ser Phe Ala Ser Ala Glu Tyr Gln Glu Ala 
    450                 455                 460                 


Leu Ser Arg Ile Thr Ser Val Val Gln Gln His Gln Glu Ala Leu Val 
465                 470                 475                 480 


Pro Ala Gln Pro Gln Leu Asn Val Cys Asn Trp Ala Val Met Asn Trp 
                485                 490                 495     


Ala Pro Pro Gly Cys Glu Lys Leu Gly Lys Cys Leu Thr Pro Pro Glu 
            500                 505                 510         


Ser Ile Pro Lys Lys Cys Leu Trp Ser His 
        515                 520         


<210>  5
<211>  446
<212>  PRT
<213>  Homo sapiens

<400>  5

Ala Pro Gln Pro Pro Asn Ile Leu Leu Leu Leu Met Asp Asp Met Gly 
1               5                   10                  15      


Trp Gly Asp Leu Gly Val Tyr Gly Glu Pro Ser Arg Glu Thr Pro Asn 
            20                  25                  30          


Leu Asp Arg Met Ala Ala Glu Gly Leu Leu Phe Pro Asn Phe Tyr Ser 
        35                  40                  45              


Ala Asn Pro Leu Cys Ser Pro Ser Arg Ala Ala Leu Leu Thr Gly Arg 
    50                  55                  60                  


Leu Pro Ile Arg Asn Gly Phe Tyr Thr Thr Asn Ala His Ala Arg Asn 
65                  70                  75                  80  


Ala Tyr Thr Pro Gln Glu Ile Val Gly Gly Ile Pro Asp Ser Glu Gln 
                85                  90                  95      


Leu Leu Pro Glu Asn Ile Pro Val Tyr Arg Asp Trp Glu Met Val Gly 
            100                 105                 110         


Arg Tyr Tyr Glu Glu Phe Pro Ile Asn Leu Lys Thr Gly Glu Ala Asn 
        115                 120                 125             


Leu Thr Gln Ile Tyr Leu Gln Glu Ala Leu Asp Phe Ile Lys Arg Gln 
    130                 135                 140                 


Ala Arg His His Pro Phe Phe Leu Tyr Trp Ala Val Asp Ala Thr His 
145                 150                 155                 160 


Ala Arg Val Tyr Ala Ser Lys Pro Phe Leu Gly Thr Ser Gln Arg Gly 
                165                 170                 175     


Arg Tyr Gly Asp Ala Val Arg Glu Ile Asp Asp Ser Ile Gly Lys Ile 
            180                 185                 190         


Leu Glu Leu Leu Gln Asp Leu His Val Ala Asp Asn Thr Phe Val Phe 
        195                 200                 205             


Phe Thr Ser Asp Asn Gly Ala Ala Leu Ile Ser Ala Pro Glu Gln Gly 
    210                 215                 220                 


Gly Ser Asn Gly Pro Phe Leu Cys Gly Lys Gln Thr Thr Phe Glu Gly 
225                 230                 235                 240 


Gly Met Arg Glu Pro Ala Leu Ala Trp Trp Pro Gly His Val Thr Ala 
                245                 250                 255     


Gly Gln Val Ser His Gln Leu Gly Ser Ile Met Asp Leu Phe Thr Thr 
            260                 265                 270         


Ser Leu Ala Leu Ala Gly Leu Thr Pro Pro Ser Asp Arg Ala Ile Asp 
        275                 280                 285             


Gly Leu Asn Leu Leu Pro Thr Leu Leu Gln Gly Arg Leu Met Asp Arg 
    290                 295                 300                 


Pro Ile Phe Tyr Tyr Arg Gly Asp Thr Leu Met Ala Ala Thr Leu Gly 
305                 310                 315                 320 


Gln His Lys Ala His Phe Trp Thr Trp Thr Asn Ser Trp Glu Asn Phe 
                325                 330                 335     


Arg Gln Gly Ile Asp Phe Cys Pro Gly Gln Asn Val Ser Gly Val Thr 
            340                 345                 350         


Thr His Asn Leu Glu Asp His Thr Lys Leu Pro Leu Ile Phe His Leu 
        355                 360                 365             


Gly Arg Asp Pro Gly Glu Arg Phe Pro Leu Ser Phe Ala Ser Ala Glu 
    370                 375                 380                 


Tyr Gln Glu Ala Leu Ser Arg Ile Thr Ser Val Val Gln Gln His Gln 
385                 390                 395                 400 


Glu Ala Leu Val Pro Ala Gln Pro Gln Leu Asn Val Cys Asn Trp Ala 
                405                 410                 415     


Val Met Asn Trp Ala Pro Pro Gly Cys Glu Lys Leu Gly Lys Cys Leu 
            420                 425                 430         


Thr Pro Pro Glu Ser Ile Pro Lys Lys Cys Leu Trp Ser His 
        435                 440                 445     


