
                                SEQUENCE LISTING

<110> Pioneer Hi-Bred International, Inc.

<120> Novel Bacillus Thuringiensis Gene with Coleopteran Activity
  

<130> 2659-PCT

<150> 61/020,169
<151> 2008-01-10

<160> 2

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 3468
<212> DNA
<213> Bacillus thuringiensis

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> GS080

<400> 1
atgaatagaa atgaatataa taaaaaagaa gtaaagccct cttcatcgaa tctttctccc 60
aaatacccac tgatgaattg tcttgatacg aaaagtcaaa atgttaatta taaagatagt 120
ttgaatgtta taaaaggcga ttatcaagag ttagatccat caacgtcgac aagagcagct 180
tctgatgcga ttactgctgc ccttagtatt acctcaacaa tgttaggggc attaggtcac 240
ccaattactg gtgcgcttct aggcgttttt aatacactga caggattgtt gtggccaggc 300
gggggagatc tggagattaa aacgctgctg atggacgaac aggatcacgg ctatgcgctg 360
acgggtgatg cgctttctca ggcagcgatt gcggcggcaa accgttcgca catgccttac 420
agtaagtcgc caagcggtgt cgcgctggaa tgtaaagacg gtcgtatttt cagtggcagc 480
tacgctgaaa acgccgcatt caacccgact ctgccaccgt tgcagggagc gttaattctg 540
ttgaatctca agggttatga ttacccggat atcacaatat tcggggaaga atggggattc 600
cctgaaacag acattaattt ttattataaa tcagaatttt tagaacatat agaggaatac 660
actgattatg ttgtcaaatg gtataaagaa gggttaaatg aattacagaa gtcatcgtct 720
acagattggg ttgcatataa tcgttatcga agagaaatga ctatattggc actggatatt 780
attgcactct ttccggcata tgatggattt ttatatccat tggagacaaa cactgagttg 840
acaagagaag tgtatatgga ccctgaaggt ggcggaccaa ctaactggcg tcaataccca 900
atctctttca cgcaaataga aaatttaatt cgtccgcctc atttatttac ttggctaaat 960
tccatacaaa ttgaaactga caaggaatta actgcggacg attattatgt atgtatgtgg 1020
gcaggtagtt tgctaggctt gcattataca aattcgtcaa gtgttttcac agtgaaaaat 1080
ggcagtggtg actataaaaa gctttatgat ctctcaggga aagatgttta tcaaacgtta 1140
ttatacgcag gttcgagtca tgaagctggc tttagcaaag tcactgattt tgttggaaac 1200
gaaggtggga cttttaactt agtatctgag agtggtacca caagctcttt ttcttctgca 1260
ctttgcggaa tatcaaacac acatcttata gattcagtaa cggtattacc tccggatata 1320
tcggacccat ctcaaagtct ttcaaaggat tatactcata gactatctta tgttactaac 1380
gtgtcctatt atagaaatca gtctgttgat tttgatcgag tagagctacc catattaggt 1440
tggacccata gaagtgtagg ttttgataat cgaatttatc catataaaat tacgcaggtt 1500
ccagctgtga aagctaataa actggataat agtgccatga cagctgtaga agggccaaat 1560
tttacaggtg gagccctagt taaggtaaac agcactggtg gtggtagata tagtttgaaa 1620
ttttctgtta aggcagatcc aaataactta tcgcaaaaat attatataag attacgatat 1680
gcaggtgaat ggcgtttcaa tcaaagtaat tatagaagtt ttactattag tattaacggc 1740
aataaaattg agggtaaagg atttttaagt aatactagat tggaaaccga tgaagttcct 1800
acgcttttta atgactttgg gtggaccagt attaaatcta cctttacttt tccaaaatcg 1860
gaatgtgaaa taagtttgga tttttatgat gagaattcaa ctattggacc agttgggcca 1920
gtattcattg atagaattga ggttgtccca gcggatgata attacggaga aaaagaaaat 1980
ttagaaaaag cacagaaagc cgtgaatgcc ttgtttacag cgggaagaca tgcactccaa 2040
acaggtgtga cagattacaa agtggaccag gtttcaattt tagtggattg tgtatcagga 2100
gagttatatc ccaatgagaa acgcaactac aaaatcttat cgatagtcaa atacgcaaaa 2160
cgtttgagct attcccgtaa tttacttcta gatccaacat tcgattctat taattcatct 2220
gaggagaatg gttggtatgg aagtaatggt attgtgattg gaaatgggga ttttgtattc 2280
aaaggtaact atttaatttt ttcaggtacc aatgatacac aatatccaac atatctctac 2340
caaaaaatag atgaatccaa actcaaagaa tatacacgct ataaactgaa aggttttatc 2400
gaaagtagtc aggatttaga agcttatgtg attcgctatg atgcaaaaca tagaacattg 2460
gatgtttctg ataatctatt accagatatt ctccctgaga atacatgtgg agaaccaaat 2520
cgctgcgcgg cacaacaata cctggatgaa aatccaagtt cagaatgtag ttcgatgcaa 2580
gatggaattt tgtctgattc gcattcattt tctcttaata tagatatagg ttctattaat 2640
cacaatgaga atttaggaat ttgggtgttg tttaaaattt cgacactaga aggatatgcg 2700
aaatttggaa atctagaagt gattgaagat ggcccagtta ttggagaagc attagcccgt 2760
gtgaaacgcc aagaaacgaa gtggagaaac aagttagccc aactgacaac ggaaacacaa 2820
gcgatttata cacgagcaaa acaagcgctg gataatcttt ttgcgaatgc acaagactct 2880
cacttaaaaa tagatgttac atttgcggaa attgcggctg caagaaagat tgtccaatca 2940
atacgcgaag cgtatatgtc atggttatct gttgttccag gtgtaaatca ccctattttt 3000
acagagttaa gtgagcgagt acaacgagca tttcaattat atgatgtacg aaatgttgtg 3060
cgtaatggtc gattcctcaa tggcttatcc gattggattg taacatctga cgtaaaggta 3120
caagaagaaa atgggaataa cgtattagtt cttaacaatt gggatgcaca agtattacaa 3180
aacgtaaaac tctatcaaga ccgtgggtat atcttacgtg taacagcgcg caagatagga 3240
attggggaag gatatataac gattacggat gaagaagggc atacagttca attgagattt 3300
actgcatgtg aagtgattga tgcatctaat gcgtttatat ccggttatat tacaaaagaa 3360
ctggaattct tcccagatac agagaaagtg catatagaaa taggcgaaac agaaggaata 3420
ttcctggtag aaagtataga gttatttttg atggaagagc tatgttaa              3468

<210> 2
<211> 1155
<212> PRT
<213> Bacillus thuringiensis

<220> 
<221> PEPTIDE        
<222> (0)...(0)
<223> GS080

<400> 2
Met Asn Arg Asn Glu Tyr Asn Lys Lys Glu Val Lys Pro Ser Ser Ser
 1               5                  10                  15      
Asn Leu Ser Pro Lys Tyr Pro Leu Met Asn Cys Leu Asp Thr Lys Ser
            20                  25                  30          
Gln Asn Val Asn Tyr Lys Asp Ser Leu Asn Val Ile Lys Gly Asp Tyr
        35                  40                  45              
Gln Glu Leu Asp Pro Ser Thr Ser Thr Arg Ala Ala Ser Asp Ala Ile
    50                  55                  60                  
Thr Ala Ala Leu Ser Ile Thr Ser Thr Met Leu Gly Ala Leu Gly His
65                  70                  75                  80  
Pro Ile Thr Gly Ala Leu Leu Gly Val Phe Asn Thr Leu Thr Gly Leu
                85                  90                  95      
Leu Trp Pro Gly Gly Gly Asp Leu Glu Ile Lys Thr Leu Leu Met Asp
            100                 105                 110         
Glu Gln Asp His Gly Tyr Ala Leu Thr Gly Asp Ala Leu Ser Gln Ala
        115                 120                 125             
Ala Ile Ala Ala Ala Asn Arg Ser His Met Pro Tyr Ser Lys Ser Pro
    130                 135                 140                 
Ser Gly Val Ala Leu Glu Cys Lys Asp Gly Arg Ile Phe Ser Gly Ser
145                 150                 155                 160 
Tyr Ala Glu Asn Ala Ala Phe Asn Pro Thr Leu Pro Pro Leu Gln Gly
                165                 170                 175     
Ala Leu Ile Leu Leu Asn Leu Lys Gly Tyr Asp Tyr Pro Asp Ile Thr
            180                 185                 190         
Ile Phe Gly Glu Glu Trp Gly Phe Pro Glu Thr Asp Ile Asn Phe Tyr
        195                 200                 205             
Tyr Lys Ser Glu Phe Leu Glu His Ile Glu Glu Tyr Thr Asp Tyr Val
    210                 215                 220                 
Val Lys Trp Tyr Lys Glu Gly Leu Asn Glu Leu Gln Lys Ser Ser Ser
225                 230                 235                 240 
Thr Asp Trp Val Ala Tyr Asn Arg Tyr Arg Arg Glu Met Thr Ile Leu
                245                 250                 255     
Ala Leu Asp Ile Ile Ala Leu Phe Pro Ala Tyr Asp Gly Phe Leu Tyr
            260                 265                 270         
Pro Leu Glu Thr Asn Thr Glu Leu Thr Arg Glu Val Tyr Met Asp Pro
        275                 280                 285             
Glu Gly Gly Gly Pro Thr Asn Trp Arg Gln Tyr Pro Ile Ser Phe Thr
    290                 295                 300                 
Gln Ile Glu Asn Leu Ile Arg Pro Pro His Leu Phe Thr Trp Leu Asn
305                 310                 315                 320 
Ser Ile Gln Ile Glu Thr Asp Lys Glu Leu Thr Ala Asp Asp Tyr Tyr
                325                 330                 335     
Val Cys Met Trp Ala Gly Ser Leu Leu Gly Leu His Tyr Thr Asn Ser
            340                 345                 350         
Ser Ser Val Phe Thr Val Lys Asn Gly Ser Gly Asp Tyr Lys Lys Leu
        355                 360                 365             
Tyr Asp Leu Ser Gly Lys Asp Val Tyr Gln Thr Leu Leu Tyr Ala Gly
    370                 375                 380                 
Ser Ser His Glu Ala Gly Phe Ser Lys Val Thr Asp Phe Val Gly Asn
385                 390                 395                 400 
Glu Gly Gly Thr Phe Asn Leu Val Ser Glu Ser Gly Thr Thr Ser Ser
                405                 410                 415     
Phe Ser Ser Ala Leu Cys Gly Ile Ser Asn Thr His Leu Ile Asp Ser
            420                 425                 430         
Val Thr Val Leu Pro Pro Asp Ile Ser Asp Pro Ser Gln Ser Leu Ser
        435                 440                 445             
Lys Asp Tyr Thr His Arg Leu Ser Tyr Val Thr Asn Val Ser Tyr Tyr
    450                 455                 460                 
Arg Asn Gln Ser Val Asp Phe Asp Arg Val Glu Leu Pro Ile Leu Gly
465                 470                 475                 480 
Trp Thr His Arg Ser Val Gly Phe Asp Asn Arg Ile Tyr Pro Tyr Lys
                485                 490                 495     
Ile Thr Gln Val Pro Ala Val Lys Ala Asn Lys Leu Asp Asn Ser Ala
            500                 505                 510         
Met Thr Ala Val Glu Gly Pro Asn Phe Thr Gly Gly Ala Leu Val Lys
        515                 520                 525             
Val Asn Ser Thr Gly Gly Gly Arg Tyr Ser Leu Lys Phe Ser Val Lys
    530                 535                 540                 
Ala Asp Pro Asn Asn Leu Ser Gln Lys Tyr Tyr Ile Arg Leu Arg Tyr
545                 550                 555                 560 
Ala Gly Glu Trp Arg Phe Asn Gln Ser Asn Tyr Arg Ser Phe Thr Ile
                565                 570                 575     
Ser Ile Asn Gly Asn Lys Ile Glu Gly Lys Gly Phe Leu Ser Asn Thr
            580                 585                 590         
Arg Leu Glu Thr Asp Glu Val Pro Thr Leu Phe Asn Asp Phe Gly Trp
        595                 600                 605             
Thr Ser Ile Lys Ser Thr Phe Thr Phe Pro Lys Ser Glu Cys Glu Ile
    610                 615                 620                 
Ser Leu Asp Phe Tyr Asp Glu Asn Ser Thr Ile Gly Pro Val Gly Pro
625                 630                 635                 640 
Val Phe Ile Asp Arg Ile Glu Val Val Pro Ala Asp Asp Asn Tyr Gly
                645                 650                 655     
Glu Lys Glu Asn Leu Glu Lys Ala Gln Lys Ala Val Asn Ala Leu Phe
            660                 665                 670         
Thr Ala Gly Arg His Ala Leu Gln Thr Gly Val Thr Asp Tyr Lys Val
        675                 680                 685             
Asp Gln Val Ser Ile Leu Val Asp Cys Val Ser Gly Glu Leu Tyr Pro
    690                 695                 700                 
Asn Glu Lys Arg Asn Tyr Lys Ile Leu Ser Ile Val Lys Tyr Ala Lys
705                 710                 715                 720 
Arg Leu Ser Tyr Ser Arg Asn Leu Leu Leu Asp Pro Thr Phe Asp Ser
                725                 730                 735     
Ile Asn Ser Ser Glu Glu Asn Gly Trp Tyr Gly Ser Asn Gly Ile Val
            740                 745                 750         
Ile Gly Asn Gly Asp Phe Val Phe Lys Gly Asn Tyr Leu Ile Phe Ser
        755                 760                 765             
Gly Thr Asn Asp Thr Gln Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp
    770                 775                 780                 
Glu Ser Lys Leu Lys Glu Tyr Thr Arg Tyr Lys Leu Lys Gly Phe Ile
785                 790                 795                 800 
Glu Ser Ser Gln Asp Leu Glu Ala Tyr Val Ile Arg Tyr Asp Ala Lys
                805                 810                 815     
His Arg Thr Leu Asp Val Ser Asp Asn Leu Leu Pro Asp Ile Leu Pro
            820                 825                 830         
Glu Asn Thr Cys Gly Glu Pro Asn Arg Cys Ala Ala Gln Gln Tyr Leu
        835                 840                 845             
Asp Glu Asn Pro Ser Ser Glu Cys Ser Ser Met Gln Asp Gly Ile Leu
    850                 855                 860                 
Ser Asp Ser His Ser Phe Ser Leu Asn Ile Asp Ile Gly Ser Ile Asn
865                 870                 875                 880 
His Asn Glu Asn Leu Gly Ile Trp Val Leu Phe Lys Ile Ser Thr Leu
                885                 890                 895     
Glu Gly Tyr Ala Lys Phe Gly Asn Leu Glu Val Ile Glu Asp Gly Pro
            900                 905                 910         
Val Ile Gly Glu Ala Leu Ala Arg Val Lys Arg Gln Glu Thr Lys Trp
        915                 920                 925             
Arg Asn Lys Leu Ala Gln Leu Thr Thr Glu Thr Gln Ala Ile Tyr Thr
    930                 935                 940                 
Arg Ala Lys Gln Ala Leu Asp Asn Leu Phe Ala Asn Ala Gln Asp Ser
945                 950                 955                 960 
His Leu Lys Ile Asp Val Thr Phe Ala Glu Ile Ala Ala Ala Arg Lys
                965                 970                 975     
Ile Val Gln Ser Ile Arg Glu Ala Tyr Met Ser Trp Leu Ser Val Val
            980                 985                 990         
Pro Gly Val Asn His Pro Ile Phe Thr Glu Leu Ser Glu Arg Val Gln
        995                 1000                1005            
Arg Ala Phe Gln Leu Tyr Asp Val Arg Asn Val Val Arg Asn Gly Arg
    1010                1015                1020                
Phe Leu Asn Gly Leu Ser Asp Trp Ile Val Thr Ser Asp Val Lys Val
1025                1030                1035               1040 
Gln Glu Glu Asn Gly Asn Asn Val Leu Val Leu Asn Asn Trp Asp Ala
                1045                1050                1055    
Gln Val Leu Gln Asn Val Lys Leu Tyr Gln Asp Arg Gly Tyr Ile Leu
            1060                1065                1070        
Arg Val Thr Ala Arg Lys Ile Gly Ile Gly Glu Gly Tyr Ile Thr Ile
        1075                1080                1085            
Thr Asp Glu Glu Gly His Thr Val Gln Leu Arg Phe Thr Ala Cys Glu
    1090                1095                1100                
Val Ile Asp Ala Ser Asn Ala Phe Ile Ser Gly Tyr Ile Thr Lys Glu
1105                1110                1115               1120 
Leu Glu Phe Phe Pro Asp Thr Glu Lys Val His Ile Glu Ile Gly Glu
                1125                1130                1135    
Thr Glu Gly Ile Phe Leu Val Glu Ser Ile Glu Leu Phe Leu Met Glu
            1140                1145                1150        
Glu Leu Cys
        1155

