
                                SEQUENCE LISTING

<110> Pioneer Hi-Bred International, inc.

<120> Novel Bacillus Thuringiensis Gene with
  Coleopteran Activity

<130> 2584-PCT

<150> 61/020,166
<151> 2008-01-10


<160> 2

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 2160
<212> DNA
<213> Bacillus thuringiensis

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> GS063

<400> 1
atggagaata atattcaaaa tcaatgcgta ccttataatt gtctaagcac ccctgagaaa 60
atactattag atgaggaaag aattgagact gggaatacat caatcgatct ttctttgtcg 120
cttgtgagcc ttcttttagg tgaattcgtc cctggtgcgt catttgtact aggtctaatt 180
gatataatat ggggatttgc aggtccctct caatgggacg catttctggt acagattgaa 240
cagttaattg acgaaagaat aggtcagttc gcaaggaatc aagcaatttc tagattagaa 300
gggctaagca atctctatca aatatacgca gaagatttta cacagtggga agcagatccc 360
gataatccag cattaagaga agagatgcgt actcaattca atgatatgaa cagtgctctt 420
acaaccgcta ttcctctttt ggcagttcaa aactatcaaa ttcctctttt atcagtatat 480
gttcaagctg caaatttaca tttatcagtt ttgagagatg tttcagtgtt tggacaaagt 540
tggggatttg atgcggcgac tattaatagc cgttataatg atttaactag gcttattagc 600
agctatacag atcatgtagt aagatggtat gatacaggat tagaccgttt gcgaggctct 660
acttaccaag actggtttag atacaaccga tttagaagag aattaacatt gactgcatta 720
gatatcgttg ctcttttccc aaactatgat atcaaaatgt atccaatcca acccgttagc 780
caactaacaa gggaagttta tacggaccca ttaattaatt tcaatccgca gttacagtct 840
gtagctcaat tgcctacttt taacgttatg gaaagtaacg caattagaaa ccctcattta 900
gttgacttct tgaataacct tagaattttt acagattggt ttagtgtcgg acggcactat 960
tattggggag gacatcgagt gatttccaaa cgtgtaggag gaagggagat aaccttccct 1020
atatatggaa gggaggcaaa gcaggaacct ccaagatcct ttacttttaa tggacctgtt 1080
tttaggacgt tatcaaatcc taccctaaga ccattacaac aacctgcacc agctcctcct 1140
tttaatttac gtggcttgga aggtgtaaaa ttttatacac ctacaaatac ctttacgtat 1200
cggggaagag gcccgcgtga ttctttaact gaattaccgc ctggagatac aagtgtacta 1260
cctcgcgaag gatatagtca ccggttatgt catgcaacat ttattcaaag atctggcaca 1320
ccttttttaa caacaggcgt agtcttttct tggacacatc gtagtgctga tgaaacgaat 1380
ataatttatc cagataagat tactcaaatt ccatgggtaa aggcgcatac ccttgaatcg 1440
ggggccactg ttattaaggg tcctggattt acaggagggg atattcttac tgttcttact 1500
agtcttggtt ccttgggcgc tttacgagta acttttacgg ggcaattacc acaaacatat 1560
aatatacgaa tccgatatgc ctcggtgcta aataaatatg gtacactcca tttttcacag 1620
ccacctgcat atgggctcac atttccaaaa actatggata tagatgaacc attaacatct 1680
cgctcgtttg cttttacaac tctttggaca ccaataacct ttacacgagc acaagaggaa 1740
tttaatctaa caatacaatc aggtgtttat atagatagaa ttgaatttgt tccggcagaa 1800
gtaacatttg aggcagacta tgacttggaa aaagcgcaaa aggcggtgaa tgctttgttt 1860
acttctaaaa accaaagggg gctaaaaaca gaggtgacgg attatcatat tgatcaagta 1920
tctaatttag tcgaatgttt atccgatgag ttttgcctag atgaaaagcg agaattacct 1980
gagaaagtca aacaggcgaa gcgactcagt gatgagcgaa acctacttca agattcaaaa 2040
tttaggggaa tcaacaggca accagacagc ggatggagag gaagtacgga tattaccatc 2100
caaggaggag atgacgtatt caaagagaat tacgtcacac taacaggtac ctttgatgag 2160


<210> 2
<211> 720
<212> PRT
<213> Bacillus thuringiensis

<220> 
<221> PEPTIDE        
<222> (0)...(0)
<223> GS063

<400> 2
Met Glu Asn Asn Ile Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser
 1               5                  10                  15      
Thr Pro Glu Lys Ile Leu Leu Asp Glu Glu Arg Ile Glu Thr Gly Asn
            20                  25                  30          
Thr Ser Ile Asp Leu Ser Leu Ser Leu Val Ser Leu Leu Leu Gly Glu
        35                  40                  45              
Phe Val Pro Gly Ala Ser Phe Val Leu Gly Leu Ile Asp Ile Ile Trp
    50                  55                  60                  
Gly Phe Ala Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile Glu
65                  70                  75                  80  
Gln Leu Ile Asp Glu Arg Ile Gly Gln Phe Ala Arg Asn Gln Ala Ile
                85                  90                  95      
Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu Asp
            100                 105                 110         
Phe Thr Gln Trp Glu Ala Asp Pro Asp Asn Pro Ala Leu Arg Glu Glu
        115                 120                 125             
Met Arg Thr Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala Ile
    130                 135                 140                 
Pro Leu Leu Ala Val Gln Asn Tyr Gln Ile Pro Leu Leu Ser Val Tyr
145                 150                 155                 160 
Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser Val
                165                 170                 175     
Phe Gly Gln Ser Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg Tyr
            180                 185                 190         
Asn Asp Leu Thr Arg Leu Ile Ser Ser Tyr Thr Asp His Val Val Arg
        195                 200                 205             
Trp Tyr Asp Thr Gly Leu Asp Arg Leu Arg Gly Ser Thr Tyr Gln Asp
    210                 215                 220                 
Trp Phe Arg Tyr Asn Arg Phe Arg Arg Glu Leu Thr Leu Thr Ala Leu
225                 230                 235                 240 
Asp Ile Val Ala Leu Phe Pro Asn Tyr Asp Ile Lys Met Tyr Pro Ile
                245                 250                 255     
Gln Pro Val Ser Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Leu Ile
            260                 265                 270         
Asn Phe Asn Pro Gln Leu Gln Ser Val Ala Gln Leu Pro Thr Phe Asn
        275                 280                 285             
Val Met Glu Ser Asn Ala Ile Arg Asn Pro His Leu Val Asp Phe Leu
    290                 295                 300                 
Asn Asn Leu Arg Ile Phe Thr Asp Trp Phe Ser Val Gly Arg His Tyr
305                 310                 315                 320 
Tyr Trp Gly Gly His Arg Val Ile Ser Lys Arg Val Gly Gly Arg Glu
                325                 330                 335     
Ile Thr Phe Pro Ile Tyr Gly Arg Glu Ala Lys Gln Glu Pro Pro Arg
            340                 345                 350         
Ser Phe Thr Phe Asn Gly Pro Val Phe Arg Thr Leu Ser Asn Pro Thr
        355                 360                 365             
Leu Arg Pro Leu Gln Gln Pro Ala Pro Ala Pro Pro Phe Asn Leu Arg
    370                 375                 380                 
Gly Leu Glu Gly Val Lys Phe Tyr Thr Pro Thr Asn Thr Phe Thr Tyr
385                 390                 395                 400 
Arg Gly Arg Gly Pro Arg Asp Ser Leu Thr Glu Leu Pro Pro Gly Asp
                405                 410                 415     
Thr Ser Val Leu Pro Arg Glu Gly Tyr Ser His Arg Leu Cys His Ala
            420                 425                 430         
Thr Phe Ile Gln Arg Ser Gly Thr Pro Phe Leu Thr Thr Gly Val Val
        435                 440                 445             
Phe Ser Trp Thr His Arg Ser Ala Asp Glu Thr Asn Ile Ile Tyr Pro
    450                 455                 460                 
Asp Lys Ile Thr Gln Ile Pro Trp Val Lys Ala His Thr Leu Glu Ser
465                 470                 475                 480 
Gly Ala Thr Val Ile Lys Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu
                485                 490                 495     
Thr Val Leu Thr Ser Leu Gly Ser Leu Gly Ala Leu Arg Val Thr Phe
            500                 505                 510         
Thr Gly Gln Leu Pro Gln Thr Tyr Asn Ile Arg Ile Arg Tyr Ala Ser
        515                 520                 525             
Val Leu Asn Lys Tyr Gly Thr Leu His Phe Ser Gln Pro Pro Ala Tyr
    530                 535                 540                 
Gly Leu Thr Phe Pro Lys Thr Met Asp Ile Asp Glu Pro Leu Thr Ser
545                 550                 555                 560 
Arg Ser Phe Ala Phe Thr Thr Leu Trp Thr Pro Ile Thr Phe Thr Arg
                565                 570                 575     
Ala Gln Glu Glu Phe Asn Leu Thr Ile Gln Ser Gly Val Tyr Ile Asp
            580                 585                 590         
Arg Ile Glu Phe Val Pro Ala Glu Val Thr Phe Glu Ala Asp Tyr Asp
        595                 600                 605             
Leu Glu Lys Ala Gln Lys Ala Val Asn Ala Leu Phe Thr Ser Lys Asn
    610                 615                 620                 
Gln Arg Gly Leu Lys Thr Glu Val Thr Asp Tyr His Ile Asp Gln Val
625                 630                 635                 640 
Ser Asn Leu Val Glu Cys Leu Ser Asp Glu Phe Cys Leu Asp Glu Lys
                645                 650                 655     
Arg Glu Leu Pro Glu Lys Val Lys Gln Ala Lys Arg Leu Ser Asp Glu
            660                 665                 670         
Arg Asn Leu Leu Gln Asp Ser Lys Phe Arg Gly Ile Asn Arg Gln Pro
        675                 680                 685             
Asp Ser Gly Trp Arg Gly Ser Thr Asp Ile Thr Ile Gln Gly Gly Asp
    690                 695                 700                 
Asp Val Phe Lys Glu Asn Tyr Val Thr Leu Thr Gly Thr Phe Asp Glu
705                 710                 715                 720 

