
                                SEQUENCE LISTING

<110> Pioneer Hi-Bred International, Inc.

<120> Novel Bacillus Thuringiensis Gene with
  Coleopteran Activity

<130> 2572-PCT

<150> 61/020,161
<151> 2008-01-10

<160> 2

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 2190
<212> DNA
<213> Bacillus thuringiensis

<220> 
<221> misc_feature   
<222> (0)...(0)
<223> GS006

<400> 1
atgaatagaa atgaatataa taaaaaagaa gtaaagccct cttcatcgaa tctttctccc 60
aaatacccac tgatgaattg tcttgatacg aaaagtcaaa atgttaatta taaagatagt 120
ttgaatgtta taaaaggcga ttatcaagag ttagatccat caacgtcgac aagagcagct 180
tctgatgcga ttactgctgc ccttagtatt acctcaacaa tgttaggggc attaggtcac 240
ccaattactg gtgcgcttct aggcgttttt aatacactga caggattgtt gtggccaggc 300
gggggagatc ttacatggga agaattgttg gcggaaggtg aagaaatcat taataaaact 360
ataactgaaa gtataaaaaa tgatgctttg ctgcgattag aggaagcacg caaacaggta 420
gatgagtatt ctggggcttt aaatgattgg atattaaatc ctggtgtacg tattagtgct 480
gcaacaatga ggagtatttt tataactact gtacagcaat tagcagtaca gatggtatat 540
tttgaaaggg acaattatca aatccctctg ttaccagtat atgcacaagc tgcaaatcta 600
cacttagttg tattgaaaga tatcacaata ttcggggaag aatggggatt ccctgaaaca 660
gacattaatt tttattataa atcagaattt ttagaacata tagaggaata cactgattat 720
gttgtcaaat ggtataaaga agggttaaat gaattacaga agtcatcgtc tacagattgg 780
gttgcatata atcgttatcg aagagaaatg actatattgg cactggatat tattgcactc 840
ttcccggcat atgatggatt tttatatcca ttggagacaa acactgagtt gacaagagaa 900
gtgtatatgg accctgaagg tggcggacca actaactggc gtcaataccc aatctctttc 960
acgcaaatag aaaatttaat tcgtccgcct catttattta cttggctaaa ttccatacaa 1020
attgaaactg acaaggaatt aactgcggac gattattatg tatgtatgtg ggcaggtagt 1080
ttgctaggct tgcattatac aaattcgtca agtgttttca cagtgaaaaa tggcagtggt 1140
gactataaaa agctttatga tctctcaggg aaagatgttt atcaaacgtt attatacgca 1200
ggttcgagtc atgaagctgg ctttagcaaa gtcactgatt ttgttggaaa cgaaggtggg 1260
acttttaact tagtatctga gagtggtacc acaagctctt tttcttctgc actttgcgga 1320
atatcaaaca cacatcttat agattcagta acggtattac ctccggatat atcggaccca 1380
tctcaaagtc tttcaaagga ttatactcat agactatctt atgttactaa cgtgtcctat 1440
tatagaaatc agtctgttga ttttgatcga gtagagctac ccatattagg ttggacccat 1500
agaagtgtag gttttgataa tcgaatttat ccatataaaa ttacgcaggt tccagctgtg 1560
aaagctaata aactggataa tagtgctatg acagttgtag aagggccaaa ttttacaggt 1620
ggagccctag ttaaggtaaa cagcactggt ggtggtagat atagtttgaa attttctgtt 1680
aaggcagatc caaataactt atcgcaaaaa tattatataa gattacgata tgcaggtgaa 1740
tggcgtttca atcaaagtaa ttatagaagt tttactatta gtattaacgg caataaaatt 1800
gagggtaaag gatttttaag taatactaga ttggaaaccg atgaagttcc tacgcttttt 1860
aatgactttg ggtggaccag tattaaatct acctttactt ttccaaaatc ggaatgtgaa 1920
ataagtttgg atttttatga tgagaattca actattggac cagttgggcc agtattcatt 1980
gatagaattg aggttgtccc agcggatgat aattacggag aaaaagaaaa tttagaaaaa 2040
gcacagaaag ccgtgaatgc cttgtttaca gcgggaagac atgcactcca aacaggtgtg 2100
acagattaca aagtggacca ggtttcaatt ttagtggatt gtgtatcagg agagttatat 2160
cccaatgaga aacgcgaact acaaaatcta                                  2190

<210> 2
<211> 730
<212> PRT
<213> Bacillus thuringiensis

<220> 
<221> PEPTIDE        
<222> (0)...(0)
<223> GS006

<400> 2
Met Asn Arg Asn Glu Tyr Asn Lys Lys Glu Val Lys Pro Ser Ser Ser
 1               5                  10                  15      
Asn Leu Ser Pro Lys Tyr Pro Leu Met Asn Cys Leu Asp Thr Lys Ser
            20                  25                  30          
Gln Asn Val Asn Tyr Lys Asp Ser Leu Asn Val Ile Lys Gly Asp Tyr
        35                  40                  45              
Gln Glu Leu Asp Pro Ser Thr Ser Thr Arg Ala Ala Ser Asp Ala Ile
    50                  55                  60                  
Thr Ala Ala Leu Ser Ile Thr Ser Thr Met Leu Gly Ala Leu Gly His
65                  70                  75                  80  
Pro Ile Thr Gly Ala Leu Leu Gly Val Phe Asn Thr Leu Thr Gly Leu
                85                  90                  95      
Leu Trp Pro Gly Gly Gly Asp Leu Thr Trp Glu Glu Leu Leu Ala Glu
            100                 105                 110         
Gly Glu Glu Ile Ile Asn Lys Thr Ile Thr Glu Ser Ile Lys Asn Asp
        115                 120                 125             
Ala Leu Leu Arg Leu Glu Glu Ala Arg Lys Gln Val Asp Glu Tyr Ser
    130                 135                 140                 
Gly Ala Leu Asn Asp Trp Ile Leu Asn Pro Gly Val Arg Ile Ser Ala
145                 150                 155                 160 
Ala Thr Met Arg Ser Ile Phe Ile Thr Thr Val Gln Gln Leu Ala Val
                165                 170                 175     
Gln Met Val Tyr Phe Glu Arg Asp Asn Tyr Gln Ile Pro Leu Leu Pro
            180                 185                 190         
Val Tyr Ala Gln Ala Ala Asn Leu His Leu Val Val Leu Lys Asp Ile
        195                 200                 205             
Thr Ile Phe Gly Glu Glu Trp Gly Phe Pro Glu Thr Asp Ile Asn Phe
    210                 215                 220                 
Tyr Tyr Lys Ser Glu Phe Leu Glu His Ile Glu Glu Tyr Thr Asp Tyr
225                 230                 235                 240 
Val Val Lys Trp Tyr Lys Glu Gly Leu Asn Glu Leu Gln Lys Ser Ser
                245                 250                 255     
Ser Thr Asp Trp Val Ala Tyr Asn Arg Tyr Arg Arg Glu Met Thr Ile
            260                 265                 270         
Leu Ala Leu Asp Ile Ile Ala Leu Phe Pro Ala Tyr Asp Gly Phe Leu
        275                 280                 285             
Tyr Pro Leu Glu Thr Asn Thr Glu Leu Thr Arg Glu Val Tyr Met Asp
    290                 295                 300                 
Pro Glu Gly Gly Gly Pro Thr Asn Trp Arg Gln Tyr Pro Ile Ser Phe
305                 310                 315                 320 
Thr Gln Ile Glu Asn Leu Ile Arg Pro Pro His Leu Phe Thr Trp Leu
                325                 330                 335     
Asn Ser Ile Gln Ile Glu Thr Asp Lys Glu Leu Thr Ala Asp Asp Tyr
            340                 345                 350         
Tyr Val Cys Met Trp Ala Gly Ser Leu Leu Gly Leu His Tyr Thr Asn
        355                 360                 365             
Ser Ser Ser Val Phe Thr Val Lys Asn Gly Ser Gly Asp Tyr Lys Lys
    370                 375                 380                 
Leu Tyr Asp Leu Ser Gly Lys Asp Val Tyr Gln Thr Leu Leu Tyr Ala
385                 390                 395                 400 
Gly Ser Ser His Glu Ala Gly Phe Ser Lys Val Thr Asp Phe Val Gly
                405                 410                 415     
Asn Glu Gly Gly Thr Phe Asn Leu Val Ser Glu Ser Gly Thr Thr Ser
            420                 425                 430         
Ser Phe Ser Ser Ala Leu Cys Gly Ile Ser Asn Thr His Leu Ile Asp
        435                 440                 445             
Ser Val Thr Val Leu Pro Pro Asp Ile Ser Asp Pro Ser Gln Ser Leu
    450                 455                 460                 
Ser Lys Asp Tyr Thr His Arg Leu Ser Tyr Val Thr Asn Val Ser Tyr
465                 470                 475                 480 
Tyr Arg Asn Gln Ser Val Asp Phe Asp Arg Val Glu Leu Pro Ile Leu
                485                 490                 495     
Gly Trp Thr His Arg Ser Val Gly Phe Asp Asn Arg Ile Tyr Pro Tyr
            500                 505                 510         
Lys Ile Thr Gln Val Pro Ala Val Lys Ala Asn Lys Leu Asp Asn Ser
        515                 520                 525             
Ala Met Thr Val Val Glu Gly Pro Asn Phe Thr Gly Gly Ala Leu Val
    530                 535                 540                 
Lys Val Asn Ser Thr Gly Gly Gly Arg Tyr Ser Leu Lys Phe Ser Val
545                 550                 555                 560 
Lys Ala Asp Pro Asn Asn Leu Ser Gln Lys Tyr Tyr Ile Arg Leu Arg
                565                 570                 575     
Tyr Ala Gly Glu Trp Arg Phe Asn Gln Ser Asn Tyr Arg Ser Phe Thr
            580                 585                 590         
Ile Ser Ile Asn Gly Asn Lys Ile Glu Gly Lys Gly Phe Leu Ser Asn
        595                 600                 605             
Thr Arg Leu Glu Thr Asp Glu Val Pro Thr Leu Phe Asn Asp Phe Gly
    610                 615                 620                 
Trp Thr Ser Ile Lys Ser Thr Phe Thr Phe Pro Lys Ser Glu Cys Glu
625                 630                 635                 640 
Ile Ser Leu Asp Phe Tyr Asp Glu Asn Ser Thr Ile Gly Pro Val Gly
                645                 650                 655     
Pro Val Phe Ile Asp Arg Ile Glu Val Val Pro Ala Asp Asp Asn Tyr
            660                 665                 670         
Gly Glu Lys Glu Asn Leu Glu Lys Ala Gln Lys Ala Val Asn Ala Leu
        675                 680                 685             
Phe Thr Ala Gly Arg His Ala Leu Gln Thr Gly Val Thr Asp Tyr Lys
    690                 695                 700                 
Val Asp Gln Val Ser Ile Leu Val Asp Cys Val Ser Gly Glu Leu Tyr
705                 710                 715                 720 
Pro Asn Glu Lys Arg Glu Leu Gln Asn Leu
                725                 730 

