
                                SEQUENCE LISTING

<110> CENTOCOR, INC.
      JACOBS, STEVEN

<120> ALTERNATIVE SCAFFOLD PROTEIN FUSIONS
      PHAGE DISPLAY VIA FUSION TO pIX OF M13 PHAGE
  

<130> CEN5209PCT

<140> TO BE ASSIGNED      
<141> 2008-12-19  

<150> 61/014778           
<151> 2007-12-19  

<160> 59

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> An EGFR binding peptide PHPEP190.

<400> 1
Asp Pro Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Leu Gln
 1               5                  10                  


<210> 2
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the Ti27 F:G loop segment.

<400> 2
Pro Leu Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Tyr Ala
 1               5                  10                  


<210> 3
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the Ti27 F:G loop segment.

<400> 3
Pro Leu Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Leu Met
 1               5                  10                  


<210> 4
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the Ti27 F:G loop segment.

<400> 4
Ala Asn Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Ala His
 1               5                  10                  


<210> 5
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the Ti27 F:G loop segment.

<400> 5
Ser Phe Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Gln Asn
 1               5                  10                  


<210> 6
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the Ti27 F:G loop segment.

<400> 6
Glu Gly Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Met Ser
 1               5                  10                  


<210> 7
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 7
Pro Leu Cys Thr Trp Glu Val Trp Gly Arg Glu Cys His Thr
 1               5                  10                  


<210> 8
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 8
Thr Ser Cys Thr Trp Glu Val Trp Gly Arg Glu Cys His Met
 1               5                  10                  


<210> 9
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 9
Asn Ser Cys Thr Trp Glu Val Trp Gly Arg Glu Cys His Val
 1               5                  10                  


<210> 10
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 10
Asp Ser Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Thr Leu
 1               5                  10                  


<210> 11
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 11
Asp Ser Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Ile Leu
 1               5                  10                  


<210> 12
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 12
Asp Thr Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Ser Ser
 1               5                  10                  


<210> 13
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 13
Asp Ile Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Phe Gly
 1               5                  10                  


<210> 14
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 14
Asp Leu Cys Thr Trp Glu Val Trp Gly Arg Glu Cys His Ala
 1               5                  10                  


<210> 15
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 15
Gly Gly Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Tyr Gln
 1               5                  10                  


<210> 16
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 16
Ser Val Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Asn Met
 1               5                  10                  


<210> 17
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 17
Val Val Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Ser Gln
 1               5                  10                  


<210> 18
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 18
Thr Thr Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Tyr Ser
 1               5                  10                  


<210> 19
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 19
His Ala Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Phe Gly
 1               5                  10                  


<210> 20
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 20
His Phe Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Gln Ser
 1               5                  10                  


<210> 21
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 21
Ser His Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Asn Leu
 1               5                  10                  


<210> 22
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 22
Asn Val Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Met Asn
 1               5                  10                  


<210> 23
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular EGFR-specific polypeptide inserted into
      the TeFN3 F:G loop segment.

<400> 23
Asn Asn Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Asn Trp
 1               5                  10                  


<210> 24
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular polypeptide inserted into the scaffold
      F:G loop segment.

<400> 24
Arg Arg Gly Asp Met Gly Ser
 1               5          


<210> 25
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular polypeptide inserted into the scaffold
      F:G loop segment.

<400> 25
Asp Pro Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Leu Gln
 1               5                  10                  


<210> 26
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular polypeptide inserted into the scaffold
      F:G loop segment.

<220> 
<221> unsure
<222> (1)(2)(3)(12)(13)(14)
<223> Wherein Xaa represents the stop codons of the TeFN3 construct. 


<400> 26
Xaa Xaa Xaa Thr Trp Glu Val Trp Gly Arg Glu Xaa Xaa Xaa
 1               5                  10                  


<210> 27
<211> 2
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular polypeptide inserted into the scaffold
      F:G loop segment.

<400> 27
Ala Gly
 1      


<210> 28
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular polypeptide inserted into the scaffold
      F:G loop segment.

<400> 28
Asp Pro Cys Thr Trp Glu Val Trp Gly Arg Glu Cys Leu Gln
 1               5                  10                  


<210> 29
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      particular polypeptide inserted into the scaffold
      F:G loop segment.

<220>
<221> unsure
<222> (1)(2)(3)(12)(13)(14)
<223> Wherein Xaa represents the stop codons of the Ti27 construct.


<400> 29

Xaa Xaa Xaa Thr Trp Glu Val Trp Gly Arg Glu Xaa Xaa Xaa
 1               5                  10                  


<210> 30
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is a linker sequence between
      the C-terminus of the scaffold protein and the
      N-terminus of pIX.

<400> 30
Glu Asp Ser Gly Gly Ser Gly Gly
 1               5              


<210> 31
<211> 55
<212> PRT
<213> Homo sapiens

<400> 31
Met Ala Val Phe Asn Ser Asp Ser Glu Cys Pro Leu Ser His Asp Gly
 1               5                  10                  15      
Tyr Cys Leu His Asp Gly Val Cys Met Tyr Ile Glu Ala Leu Asp Lys
            20                  25                  30          
Tyr Ala Cys Asn Cys Val Val Gly Tyr Ile Gly Glu Arg Cys Gln Tyr
        35                  40                  45              
Arg Asp Leu Lys Trp Trp Glu
    50                  55  


<210> 32
<211> 101
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 


<400> 32
Leu Ile Glu Val Glu Lys Pro Leu Tyr Gly Val Glu Val Phe Val Gly
 1               5                  10                  15      
Glu Thr Ala His Phe Glu Ile Glu Leu Ser Glu Pro Asp Val His Gly
            20                  25                  30          
Gln Trp Lys Leu Lys Gly Gln Pro Leu Thr Ala Ser Pro Asp Cys Glu
        35                  40                  45              
Ile Ile Glu Asp Gly Lys Lys His Ile Leu Ile Leu His Asn Cys Gln
    50                  55                  60                  
Leu Gly Met Thr Gly Glu Val Ser Phe Gln Ala Pro Leu Cys Thr Trp
65                  70                  75                  80  
Glu Val Trp Gly Arg Glu Cys Tyr Ala Ala Lys Ser Ala Ala Asn Leu
                85                  90                  95      
Lys Val Lys Glu Leu
            100     


<210> 33
<211> 101
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 


<400> 33
Leu Ile Glu Val Glu Lys Pro Leu Tyr Gly Val Glu Val Phe Val Gly
 1               5                  10                  15      
Glu Thr Ala His Phe Glu Ile Glu Leu Ser Glu Pro Asp Val His Gly
            20                  25                  30          
Gln Trp Lys Leu Lys Gly Gln Pro Leu Thr Ala Ser Pro Asp Cys Glu
        35                  40                  45              
Ile Ile Glu Asp Gly Lys Lys His Ile Leu Ile Leu His Asn Cys Gln
    50                  55                  60                  
Leu Gly Met Thr Gly Glu Val Ser Phe Gln Ala Pro Leu Cys Thr Trp
65                  70                  75                  80  
Glu Val Trp Gly Arg Glu Cys Leu Met Ala Lys Ser Ala Ala Asn Leu
                85                  90                  95      
Lys Val Lys Glu Leu
            100     


<210> 34
<211> 101
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 


<400> 34
Leu Ile Glu Val Glu Lys Pro Leu Tyr Gly Val Glu Val Phe Val Gly
 1               5                  10                  15      
Glu Thr Ala His Phe Glu Ile Glu Leu Ser Glu Pro Asp Val His Gly
            20                  25                  30          
Gln Trp Lys Leu Lys Gly Gln Pro Leu Thr Ala Ser Pro Asp Cys Glu
        35                  40                  45              
Ile Ile Glu Asp Gly Lys Lys His Ile Leu Ile Leu His Asn Cys Gln
    50                  55                  60                  
Leu Gly Met Thr Gly Glu Val Ser Phe Gln Ala Ala Asn Cys Thr Trp
65                  70                  75                  80  
Glu Val Trp Gly Arg Glu Cys Ala His Ala Lys Ser Ala Ala Asn Leu
                85                  90                  95      
Lys Val Lys Glu Leu
            100     


<210> 35
<211> 101
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 


<400> 35
Leu Ile Glu Val Glu Lys Pro Leu Tyr Gly Val Glu Val Phe Val Gly
 1               5                  10                  15      
Glu Thr Ala His Phe Glu Ile Glu Leu Ser Glu Pro Asp Val His Gly
            20                  25                  30          
Gln Trp Lys Leu Lys Gly Gln Pro Leu Thr Ala Ser Pro Asp Cys Glu
        35                  40                  45              
Ile Ile Glu Asp Gly Lys Lys His Ile Leu Ile Leu His Asn Cys Gln
    50                  55                  60                  
Leu Gly Met Thr Gly Glu Val Ser Phe Gln Ala Ser Phe Cys Thr Trp
65                  70                  75                  80  
Glu Val Trp Gly Arg Glu Cys Gln Asn Ala Lys Ser Ala Ala Asn Leu
                85                  90                  95      
Lys Val Lys Glu Leu
            100     


<210> 36
<211> 101
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 36
Leu Ile Glu Val Glu Lys Pro Leu Tyr Gly Val Glu Val Phe Val Gly
 1               5                  10                  15      
Glu Thr Ala His Phe Glu Ile Glu Leu Ser Glu Pro Asp Val His Gly
            20                  25                  30          
Gln Trp Lys Leu Lys Gly Gln Pro Leu Thr Ala Ser Pro Asp Cys Glu
        35                  40                  45              
Ile Ile Glu Asp Gly Lys Lys His Ile Leu Ile Leu His Asn Cys Gln
    50                  55                  60                  
Leu Gly Met Thr Gly Glu Val Ser Phe Gln Ala Glu Gly Cys Thr Trp
65                  70                  75                  80  
Glu Val Trp Gly Arg Glu Cys Met Ser Ala Lys Ser Ala Ala Asn Leu
                85                  90                  95      
Lys Val Lys Glu Leu
            100     


<210> 37
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 37
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Pro Leu Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys His Thr Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 38
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 38
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Thr Ser Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys His Met Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 39
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 39

Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Asn Ser Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys His Val Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 40
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 40
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Asp Ser Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Thr Leu Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 41
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 41
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Asp Ser Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Ile Leu Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 42
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 42
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Asp Thr Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Ser Ser Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 43
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 43
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Asp Ile Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Phe Gly Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 44
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 44
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Asp Leu Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys His Ala Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 45
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 45
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Gly Gly Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Tyr Gln Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 46
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 46
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Ser Val Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Asn Met Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 47
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 47
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Val Val Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Ser Gln Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 48
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 48
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Thr Thr Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Tyr Ser Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 49
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 49
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser His Ala Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Phe Gly Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 50
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 50
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser His Phe Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Gln Ser Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 51
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 51
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Ser His Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Asn Leu Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 52
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 52
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Asn Val Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Met Asn Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 53
<211> 100
<212> PRT
<213> Artificial Sequence

<220> 
<223> Wherein the sequence is representative of the
      amino acid sequences for an EGFR-specific scaffolds. 

<400> 53
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Asn Asn Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Asn Trp Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 54
<211> 93
<212> PRT
<213> Artificial Sequence

<220>
<223> Wherein the amino acid sequence is representative of the
	amino acid sequences for a full-length scaffold.

<400> 54
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Arg Arg Gly Asp Met Gly
65                  70                  75                  80  
Ser Ser Asn Pro Ala Lys Glu Thr Phe Thr Thr Gly Leu
                85                  90              


<210> 55
<211> 100
<212> PRT
<213> Artificial Sequence

<220>
<223> Wherein the amino acid sequence is representative of the
	amino acid sequences for a full-length scaffold.


<400> 55
Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Asp Pro Cys Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Cys Leu Gln Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 56
<211> 100
<212> PRT
<213> Artificial Sequence

<220>
<223> Wherein the amino acid sequence is representative of the
	amino acid sequences for a truncated scaffold.


<400> 56

Arg Leu Asp Ala Pro Ser Gln Ile Glu Val Lys Asp Val Thr Asp Thr
 1               5                  10                  15      
Thr Ala Leu Ile Thr Trp Phe Lys Pro Leu Ala Glu Ile Asp Gly Ile
            20                  25                  30          
Glu Leu Thr Tyr Gly Ile Lys Asp Val Pro Gly Asp Arg Thr Thr Ile
        35                  40                  45              
Asp Leu Thr Glu Asp Glu Asn Gln Tyr Ser Ile Gly Asn Leu Lys Pro
    50                  55                  60                  
Asp Thr Glu Tyr Glu Val Ser Leu Ile Ser Xaa Xaa Xaa Thr Trp Glu
65                  70                  75                  80  
Val Trp Gly Arg Glu Xaa Xaa Xaa Ser Asn Pro Ala Lys Glu Thr Phe
                85                  90                  95      
Thr Thr Gly Leu
            100 


<210> 57
<211> 89
<212> PRT
<213> Artificial Sequence

<220>
<223> Wherein the amino acid sequence is representative of the
	amino acid sequences for a full-length scaffold.


<400> 57
Leu Ile Glu Val Glu Lys Pro Leu Tyr Gly Val Glu Val Phe Val Gly
 1               5                  10                  15      
Glu Thr Ala His Phe Glu Ile Glu Leu Ser Glu Pro Asp Val His Gly
            20                  25                  30          
Gln Trp Lys Leu Lys Gly Gln Pro Leu Thr Ala Ser Pro Asp Cys Glu
        35                  40                  45              
Ile Ile Glu Asp Gly Lys Lys His Ile Leu Ile Leu His Asn Cys Gln
    50                  55                  60                  
Leu Gly Met Thr Gly Glu Val Ser Phe Gln Ala Ala Gly Ala Lys Ser
65                  70                  75                  80  
Ala Ala Asn Leu Lys Val Lys Glu Leu
                85                  


<210> 58
<211> 101
<212> PRT
<213> Artificial Sequence

<220>
<223> Wherein the amino acid sequence is representative of the
	amino acid sequences for a full-length scaffold.


<400> 58
Leu Ile Glu Val Glu Lys Pro Leu Tyr Gly Val Glu Val Phe Val Gly
 1               5                  10                  15      
Glu Thr Ala His Phe Glu Ile Glu Leu Ser Glu Pro Asp Val His Gly
            20                  25                  30          
Gln Trp Lys Leu Lys Gly Gln Pro Leu Thr Ala Ser Pro Asp Cys Glu
        35                  40                  45              
Ile Ile Glu Asp Gly Lys Lys His Ile Leu Ile Leu His Asn Cys Gln
    50                  55                  60                  
Leu Gly Met Thr Gly Glu Val Ser Phe Gln Ala Asp Pro Cys Thr Trp
65                  70                  75                  80  
Glu Val Trp Gly Arg Glu Cys Leu Gln Ala Lys Ser Ala Ala Asn Leu
                85                  90                  95      
Lys Val Lys Glu Leu
            100     


<210> 59
<211> 101
<212> PRT
<213> Artificial Sequence

<220>
<223> Wherein the amino acid sequence is representative of the
	amino acid sequences for a truncated scaffold.


<220>
<221> unsure
<222> (76)(77)(78)(87)(88)(89)
<223> Wherein Xaa represents the stop codons of the Ti27 construct.




<400> 59
Leu Ile Glu Val Glu Lys Pro Leu Tyr Gly Val Glu Val Phe Val Gly
 1               5                  10                  15      
Glu Thr Ala His Phe Glu Ile Glu Leu Ser Glu Pro Asp Val His Gly
            20                  25                  30          
Gln Trp Lys Leu Lys Gly Gln Pro Leu Thr Ala Ser Pro Asp Cys Glu
        35                  40                  45              
Ile Ile Glu Asp Gly Lys Lys His Ile Leu Ile Leu His Asn Cys Gln
    50                  55                  60                  
Leu Gly Met Thr Gly Glu Val Ser Phe Gln Ala Xaa Xaa Xaa Thr Trp
65                  70                  75                  80  
Glu Val Trp Gly Arg Glu Xaa Xaa Xaa Ala Lys Ser Ala Ala Asn Leu
                85                  90                  95      
Lys Val Lys Glu Leu
            100     


