                         SEQUENCE LISTING

<110>  SCHOFIELD, David
       DINOVO, Augustine
 
<120>  DIFFERENTIALLY FLUORESCENT YEAST BIOSENSORS FOR THE DETECTION AND
        BIODEGRADATION OF CHEMICAL AGENTS

<130>  068343.0140

<150>  US 60/976,602
<151>  2007-10-01

<160>  95    

<170>  PatentIn version 3.5

<210>  1
<211>  1020
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Yeast codon-optimized opd sequence

<400>  1
atgactatga taactaacag cggcgacagg ataaacacag tacgaggacc aattactata       60

tccgaagcag gattcacact gacccatgaa catatttgtg gatcgtcagc agggttttta      120

agagcttggc ccgagttctt tggctcaaga aaggcgttag cagagaaagc cgtaagggga      180

ttgcgaagag ctagagccgc cggtgttaga accattgttg acgtttctac attcgatatc      240

ggaagggacg tttctctact agcggaagtc tccagggctg cggacgtgca tattgttgct      300

gccactggtc tatggtttga tcctccattg tcaatgaggt tgagatcagt ggaggaactt      360

acgcaatttt tcttgcgtga gattcaatac ggtatagaag acacaggtat tagagcaggt      420

atcatcaaag tagctacaac cgggaaggct actccttttc aagaattagt tctaaaagcc      480

gcagccaggg catcccttgc taccggtgtt cctgtgacaa ctcatacggc tgcctctcag      540

cgtgatggcg aacaacaagc tgctatcttt gaaagcgaag gcttgtcgcc aagtagagtg      600

tgtattggac attctgatga tactgatgac ttgtcttact tgaccgcatt agctgccaga      660

ggttatttaa taggtttgga ccacattccc cacagtgcaa ttggtttgga ggataatgca      720

tcggctagtg ctctgttagg gattcgcagt tggcagacta gggctctgct aataaaggca      780

ttaattgacc aagggtatat gaaacagatc cttgtttcaa atgattggct tttcggtttt      840

tcatcttatg taactaatat catggatgta atggatcgcg tcaatccaga cggtatggct      900

ttcattccgt tacgtgtcat accgtttctg cgtgaaaaag gcgtccccca ggaaacgttg      960

gcaggtatta ctgttacaaa cccagccaga tttttaagcc ctacgctcag agcctcctga     1020


<210>  2
<211>  678
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Yeast codon-optimized DsRed express sequence

<400>  2
atggctagct ccgaggacgt tataaaagag ttcatgagat ttaaagtaag aatggagggc       60

agtgttaacg gtcacgaatt tgaaatagaa ggggaaggcg agggtagacc atatgaaggc      120

actcaaacag ccaaactgaa ggttactaag ggaggtccat tgccttttgc atgggatatt      180

ttgtcccctc aatttcaata cgggagtaaa gtgtatgtga aacaccccgc tgacatccct      240

gattataaga aactctcttt tccagaagga ttcaagtggg aaagagtcat gaacttcgaa      300

gacggaggtg tagtaacggt aacccaggat tcttctcttc aagatggtag ctttatctat      360

aaggttaaat tcattggagt caattttcca tcagacggtc ccgttatgca gaagaaaact      420

atgggatggg aagcctcgac agagaggttg tatccgcgtg atggggtgtt gaaaggcgaa      480

attcataagg ctttaaagtt aaaagacggt ggtcattatt tagttgaatt caaatctatt      540

tacatggcga aaaagcctgt ccaattacca ggttactact acgtggattc aaaactagat      600

attacctcac ataatgagga ttatacaatc gtcgaacaat acgaacgagc agaaggcagg      660

catcatcttt ttctatag                                                    678


<210>  3
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide derived from E. coli and S. cerevisiae

<400>  3
gtcgacagag tttacttgca ctaccacaat ttagatcaag taatcgtggg attcagcgtg       60

ggggcactga ctggatcact ttattttttt atagttggca tcataagaga gcttggttta      120

atcaattggt ttttaaaact acgtattgtc agattatttt acatgacaga ttcttacaat      180

ctggcgcctt tgacattgaa agagaattac gaggcgtatt ggaaaagaat aaatcagcga      240

tctttcaatg ataaatccaa aagggattag gttgaacatt tcatttttct tattttttca      300

tctattttag cgaggcgatc ttgagcgttt gtgtaactgg ataaaacttg gcaagctgga      360

gtagtcctta tttctcataa agaaagccga aagcctgctc tcctacagtt attaattaag      420

aacgctcgtt ttaaacagtt actgtgtaaa gcgacggtaa tcgaaaaagt atgtgacatc      480

gtgcaaaaga agataatcaa aaccatgcag aacgtagaat aaatgtgagt tctatcactt      540

gcggagataa taccctctga cgccgcgggg ccgattgttc cacggaggga aagaatcggc      600

cctcgggttt tacgagagcg ccgcgggtga actcactttt ttctcgagaa gaagtttcct      660

acggcagaat aagacccttt tccacggata atcagcggag cttctggaca taatagatgg      720

tttgtgaaaa agcttaagga aagccgcgga tttagcatgt cacccacact tagattacga      780

aagcaaggat tgcagaaaaa aagatgagat acagttttga gggcaatata taaaatatat      840

aaatatagca acattccctt ctctctgtta tgtccacaat acttctaaaa gttgtaacat      900

atatctatac ataaaaactt gtaacaaaca tataattaaa acaactagtg acaagacgga      960

agttataagt tatcttctct acagaaacaa tacgtaggat ccattaaaat gtctaaaggt     1020

gaagaattat tcactggtgt tgtcccaatt ttggttgaat tagatggtga tgttaatggt     1080

cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa attgacctta     1140

aaatttattt gtactactgg taaattgcca gttccatggc caaccttagt cactactttc     1200

ggttatggtg ttcaatgttt tgcgagatac ccagatcata tgaaacaaca tgactttttc     1260

aagtctgcca tgccagaagg ttatgttcaa gaaagaacta tttttttcaa agatgacggt     1320

aactacaaga ccagagctga agtcaagttt gaaggtgata ccttagttaa tagaatcgaa     1380

ttaaaaggta ttgattttaa agaagatggt aacattttag gtcacaaatt ggaatacaac     1440

tataactctc acaatgttta catcatggct gacaaacaaa agaatggtat caaagttaac     1500

ttcaaaatta gacacaacat tgaagatggt tctgttcaat tagctgacca ttatcaacaa     1560

aatactccaa ttggtgatgg tccagtcttg ttaccagaca accattactt atccactcaa     1620

tctgccttat ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt agaatttgtt     1680

actgctgctg gtattaccca tggtatggat gaattgtaca aataaactag t              1731


<210>  4
<211>  1714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide derived from E. coli and S. cerevisiae

<400>  4
gtcgactttc ttccttttct actatcttct cttttattat tatattatat tatcaataac       60

cctcatctct ctctctctct ctctctctct ctctctctct ctctctctct ctctctctct      120

ctctctctcc tttttttctt cttttagatt tttttgcttt tgtttaattt cattccagtt      180

tccccccccc aatattaata ttcgttccta gagcctatca cacctaatat ttcccgtata      240

cattatagta aatacctcaa gacacttatg atagattttt ttccttaact caaacctaat      300

tagctctatg cataaagaaa gttttgaata aggaaatccg catattcaaa ttatcaaaca      360

tgcacttcat ctgcaaaact tccaaaaact ttctgctcgt taatagtcaa tgtatggtaa      420

tgcgaatgag caggtacccc ttattataat gattaatact tacatcatag ttaataatct      480

tatctcaggg tacccataat ttcaaccatc cttagcttcc attaaaaaca caatgagttg      540

cgttactagc gaagcggctt atctgttaat tcttgcttgc aaacatctta gctgaaagtg      600

aaaaggcaca gccgcacctg ctgaatgctc aacgtttgta ataatccgcc tatttccgcg      660

gaatcaatag ggctcctagc aggccgccat caattttcag cgtgccgcat taaaattata      720

ttaccaagat ttccatttcc gcggctgatt cctatcaata ttaagtaatc aatcttttcc      780

tcgtgattct ttgtgatgct cattcacaga ggactaatta agacatgtag cacaatatat      840

tcatataaaa gttggtgcag ttaatgatta attgcattgt tttccttgtt tctttctgtt      900

atacctgtcg aattaaacat aaagtgtata tgaattttaa ggggcacaaa taacaaagga      960

ttatttatca cctttaatag gatccattaa aatgtctaaa ggtgaagaat tattcactgg     1020

tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc     1080

cggtgaaggt gaaggtgatg ctacttacgg taaattgacc ttaaaattta tttgtactac     1140

tggtaaattg ccagttccat ggccaacctt agtcactact ttcggttatg gtgttcaatg     1200

ttttgcgaga tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga     1260

aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc     1320

tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt     1380

taaagaagat ggtaacattt taggtcacaa attggaatac aactataact ctcacaatgt     1440

ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt aacttcaaaa ttagacacaa     1500

cattgaagat ggttctgttc aattagctga ccattatcaa caaaatactc caattggtga     1560

tggtccagtc ttgttaccag acaaccatta cttatccact caatctgcct tatccaaaga     1620

tccaaacgaa aagagagacc acatggtctt gttagaattt gttactgctg ctggtattac     1680

ccatggtatg gatgaattgt acaaataaac tagt                                 1714


<210>  5
<211>  1696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide derived from E. coli and S. cerevisiae

<400>  5
gtcgacggct gtatgtttat gattgctcga atcacgtttt tcttgttttt tcgtcaagaa       60

ttccagtcaa gttttccacc accttgaccc ttaaagcatc gacttttgtg ctcttgaatg      120

tgtttctaag aatacttgta aaggacaccc tctaatttcg tgtgcacttt tcacatatta      180

tcaagacaat cgttcctgta ctcagatgca ctgttactgt aaagactact atacaacaag      240

cgaaaaatga tgttcgaaaa cctttatttc tattttgaaa ggcatgtgtc tcgaggtcct      300

tgctttattg tgggtggtca tgccattctg taaaccttac ggtactgctc cgtctatatc      360

tttgaggttg ttatttcccc acaaatatgc gtttctaacc gaatattcat tcagtcggac      420

cggacaatag ctcttaactg cgtttaccgg agtaaatatc gtaagaattt gcatgcggtg      480

aaatacaggg aaaataagaa attacaccct aatacaaaaa gaaaactaag tttcacaata      540

cgtaaggata ttttagtggg gagaatattt cggagaataa agtttccaac tccgcggtgt      600

gaacaaccgc tcagcacgca gcgttattct cgagaaaagt ggccctgaaa taaggaaata      660

aagttactaa tgttttttcg ctgtacgata tcaaatgtga cgaagtaggc accccacgct      720

ataaattggc tactaaagtt tatgtcagta cttgggatcg ttgaaatact cggataaatt      780

atgttcctta tttttcatgg ttttcgtcat accacagttt accccagaat gagaaaggat      840

ctccttttga aataaaaagt acttaagggc aatgatattg agttgctaga cgtttggtta      900

gacgcctgtt ttgaaataaa aaagctgtct caaattaatc gagcaagcac agatcaaaca      960

agatacaaac aaagcttttc aacgtaatat ttactatcgg atccaaaagt atggctagct     1020

ccgaggacgt tataaaagag ttcatgagat ttaaagtaag aatggagggc agtgttaacg     1080

gtcacgaatt tgaaatagaa ggggaaggcg agggtagacc atatgaaggc actcaaacag     1140

ccaaactgaa ggttactaag ggaggtccat tgccttttgc atgggatatt ttgtcccctc     1200

aatttcaata cgggagtaaa gtgtatgtga aacaccccgc tgacatccct gattataaga     1260

aactctcttt tccagaagga ttcaagtggg aaagagtcat gaacttcgaa gacggaggtg     1320

tagtaacggt aacccaggat tcttctcttc aagatggtag ctttatctat aaggttaaat     1380

tcattggagt caattttcca tcagacggtc ccgttatgca gaagaaaact atgggatggg     1440

aagcctcgac agagaggttg tatccgcgtg atggggtgtt gaaaggcgaa attcataagg     1500

ctttaaagtt aaaagacggt ggtcattatt tagttgaatt caaatctatt tacatggcga     1560

aaaagcctgt ccaattacca ggttactact acgtggattc aaaactagat attacctcac     1620

ataatgagga ttatacaatc gtcgaacaat acgaacgagc agaaggcagg catcatcttt     1680

ttctataggc ggccgc                                                     1696


<210>  6
<211>  1706
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide derived from E. coli and S. cerevisiae

<400>  6
gtcgactttc actcaaccac ctccaaaaaa taacaggttc atctaaagta aaagacttta       60

acttgctctt agtttccaaa ttaaatatct gcacgatagt accatttgct ctaacggaaa      120

taaccatctg agatggatgc atgatagcag aatcaccgcc catattcttc cttgtcactt      180

cattgccttt ggccaaatcc acgattgcaa cagagtttgt accgtccttt gtttctctaa      240

cagtgacgaa gtggtcactc tcgaaagtag ttgatctgaa gtcaaggaat tgaggggaaa      300

ttcctaagga catcagatcg accaattcgg taaattcaat gggtaggtca ctcattggtt      360

agaactttcg tgataattta tttttatagt tgaatatctt ctttctctct caactctgat      420

ccggattgtc gaggtttcaa taagttactc tgaacaacta atcaaaatat ctccttattt      480

ctgtagattc cttcagttcc actttttact tttcttaatt ctctttgtat ttattcctag      540

cgacgaaaaa tgcgagatct cgaccaaaaa aagggggtag ggtaataaaa ttaaccctat      600

tattttttaa ctttaaaacc tataatgtgc taatatttta ttataaacct cctttttttg      660

cgttcaaacc ctgacacatt ttaagcccta tatttacggt attagttgat taaactccga      720

agcgaaagga attcggtcat tagcggctaa tagccgttgg ggtaaatcac ctacaagcaa      780

gtacacaaga gaacgttggc gttgttaagt caaagcacta atacattggg gctttaagag      840

tgtttataaa ggtctaacct gtaaaaatta tttaaacaac ttgaacaggc cttaaagttt      900

tcctcattcc gctcatcatc actaatattg ctctccgttt ttgaatacac acttgacact      960

aataagtatc acagaaaaaa agaaaatata ataaattagt attgcgatgg atccaaaagt     1020

atggctagct ccgaggacgt tataaaagag ttcatgagat ttaaagtaag aatggagggc     1080

agtgttaacg gtcacgaatt tgaaatagaa ggggaaggcg agggtagacc atatgaaggc     1140

actcaaacag ccaaactgaa ggttactaag ggaggtccat tgccttttgc atgggatatt     1200

ttgtcccctc aatttcaata cgggagtaaa gtgtatgtga aacaccccgc tgacatccct     1260

gattataaga aactctcttt tccagaagga ttcaagtggg aaagagtcat gaacttcgaa     1320

gacggaggtg tagtaacggt aacccaggat tcttctcttc aagatggtag ctttatctat     1380

aaggttaaat tcattggagt caattttcca tcagacggtc ccgttatgca gaagaaaact     1440

atgggatggg aagcctcgac agagaggttg tatccgcgtg atggggtgtt gaaaggcgaa     1500

attcataagg ctttaaagtt aaaagacggt ggtcattatt tagttgaatt caaatctatt     1560

tacatggcga aaaagcctgt ccaattacca ggttactact acgtggattc aaaactagat     1620

attacctcac ataatgagga ttatacaatc gtcgaacaat acgaacgagc agaaggcagg     1680

catcatcttt ttctataggc ggccgc                                          1706


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YGR035C 

<400>  7
gcgtgccata gtttcttcc                                                    19


<210>  8
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YGR035C 

<400>  8
tcgtccctga gacttactc                                                    19


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene SPS100

<400>  9
agtaccgtgg catcatggac                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene SPS100

<400>  10
gcagaaattg caccttgtgg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YOR186W 

<400>  11
tttaagtccg ccatacgagc                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YOR186W 

<400>  12
ctgcctacat cgccaatcac                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene RTA1

<400>  13
aatggctact ctgctgttcg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene RTA1

<400>  14
ctgcctacat cgccaatcac                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YLR346C 

<400>  15
tgtcccatcg ggttagtttc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YLR346C 

<400>  16
tgggcagcct tgagtaaatc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene MET28

<400>  17
aagcaggaag ggtatgaacg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene MET28

<400>  18
cagcagcaat gatagcaagc                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YLL056C 

<400>  19
cagcagcaat gatagcaagc                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YLL056C 

<400>  20
cggtcaattt cgcagcactg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene POX1

<400>  21
aagctgctct ggttaccgtg                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene POX1

<400>  22
atacccatgt cctccgcaag                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene HXT9

<400>  23
aagttgtggc ctcaaggaag                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene HXT9

<400>  24
tgttgcgatt gccattcctc                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YGR287C 

<400>  25
acttggcaat ccagtgatcc                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YGR287C 

<400>  26
tcggaggcat gttgcatttc                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YHL012W 

<400>  27
ttgccttctt tccgaacagt                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YHL012W 

<400>  28
gcccaagtcg attgaaggta                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene ACT1

<400>  29
ttccagcctt ctacgtttcc                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene ACT1

<400>  30
acgtgagtaa caccatcacc                                                   20


<210>  31
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YGR035C 

<400>  31
ttaagtcgac agagtttact tgcactacc                                         29


<210>  32
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YGR035C 

<400>  32
ttaagtcgac ggagataata ccctctg                                           27


<210>  33
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YGR035C 

<400>  33
aattggatcc tacgtattgt ttctgtagag                                        30


<210>  34
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YLR346C 

<400>  34
aattgtcgac tttcttcctt ttctactatc                                        30


<210>  35
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YLR346C 

<400>  35
ttaagtcgac aaccatcctt agcttcc                                           27


<210>  36
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YLR346C 

<400>  36
ttatggatcc tattaaaggt gataaataat cc                                     32


<210>  37
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene POX1

<400>  37
aattgtcgac tttcactcaa ccacctcc                                          28


<210>  38
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene POX1

<400>  38
tatagtcgac tagattcctt cagttccac                                         29


<210>  39
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene POX1

<400>  39
ttaaggatcc atcgcaatac taatttatta tat                                    33


<210>  40
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene HXT9

<400>  40
ttaagtcgac acttgggttc tgtttctgg                                         29


<210>  41
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene HXT9

<400>  41
attagtcgac atacggagcg taaacaacc                                         29


<210>  42
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene HXT9

<400>  42
atatggatcc gatattgggt aagatacgaa                                        30


<210>  43
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YLR287C 

<400>  43
ttaagtcgac ggctgtatgt ttatgattgc                                        30


<210>  44
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YLR287C 

<400>  44
ttaagtcgac gctcttaact gcgtttacc                                         29


<210>  45
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YLR287C 

<400>  45
ttaaggatcc gatagtaaat attacgttga                                        30


<210>  46
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YHL012W 

<400>  46
tattgtcgac gcctcttctt tcattttagc                                        30


<210>  47
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YHL012W 

<400>  47
attagtcgac attagatgct ttcgagatgc                                        30


<210>  48
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer directed to gene YHL012W 

<400>  48
taatggatcc tgatcttgta tatactatgt ag                                     32


<210>  49
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 

<400>  49
gagttagtgt taagggcggc cgcccgg                                           27


<210>  50
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 

<400>  50
gcaagtcagc gcggtggtga gcagcag                                           27


<210>  51
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 

<400>  51
gacgtgatgg atagcgtgaa ccccgac                                           27


<210>  52
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 

<400>  52
gccctcctgg gcaaccgttc gtggcaaac                                         29


<210>  53
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 

<400>  53
cgcaaagctc tagtggaaaa ggctgtg                                           27


<210>  54
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n is a, c, g, or t

<400>  54
gaccacatcc cgnnnagtgc gattggtc                                          28


<210>  55
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n is a, c, g, or t

<400>  55
gaccggcttg tggnnngacc cgccactttc                                        30


<210>  56
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  n is a, c, g, or t

<400>  56
tgtcgacttt cgatnnnggt cgcgacgtc                                         29


<210>  57
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (12)..(14)
<223>  n is a, c, g, or t

<400>  57
cgaccggctt gnnnttcgac ccgcc                                             25


<210>  58
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  n is a, c, g, or t

<400>  58
tcatcggtct agacnnnatc ccgtggagtg                                        30


<210>  59
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (16)..(18)
<223>  n is a, c, g, or t

<400>  59
actcacgagc acatcnnngg cagctcggca g                                      31


<210>  60
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  n is a, c, g, or t

<400>  60
cgagtgcatc agccnnnctg ggcaaccgtt c                                      31


<210>  61
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n is a, c, g, or t

<400>  61
tcgaatgact ggnnnttcgg gttttcg                                           27


<210>  62
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n is a, c, g, or t

<400>  62
gggttttcga gcnnngtcac caacatc                                           27


<210>  63
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  n is a, c, g, or t

<400>  63
catcatggac gtgnnngata gcgtgaacc                                         29


<210>  64
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n is a, c, g, or t

<400>  64
gagcacatct gcnnnagctc ggcagg                                            26


<210>  65
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (11)..(13)
<223>  n is a, c, g, or t

<400>  65
gctgttcggg nnntcgagct atgtc                                             25


<210>  66
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  n is a, c, g, or t

<400>  66
tgttcgggtt ttcgnnntat gtcaccaaca tc                                     32


<210>  67
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Opd mutagenesis oligonucleotide 


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n is a, c, g, or t

<400>  67
cacatctgcg tcnnntcggc aggattc                                           27


<210>  68
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized wild-type OPH Enzyme 

<400>  68

Met Ile Thr Asn Ser Gly Asp Arg Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Thr Ile Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Ala Glu Lys Ala Val Arg Gly Leu Arg Arg Ala Arg Ala 
    50                  55                  60                  


Ala Gly Val Arg Thr Ile Val Asp Val Ser Thr Phe Asp Ile Gly Arg 
65                  70                  75                  80  


Asp Val Ser Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Phe Asp Pro Pro Leu Ser Met Arg Leu 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln Tyr 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Lys Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ala Ala 
                165                 170                 175     


Ser Gln Arg Asp Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Ala Leu Ala Ala Arg Gly Tyr Leu Ile Gly Leu 
    210                 215                 220                 


Asp His Ile Pro His Ser Ala Ile Gly Leu Glu Asp Asn Ala Ser Ala 
225                 230                 235                 240 


Ser Ala Leu Leu Gly Ile Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Gln Gly Tyr Met Lys Gln Ile Leu Val Ser Asn 
            260                 265                 270         


Asp Trp Leu Phe Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Arg Val Asn Pro Asp Gly Met Ala Phe Ile Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Gln Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Ile Thr Val Thr Asn Pro Ala Arg Phe Leu Ser Pro Thr Leu Arg Ala 
                325                 330                 335     


Ser Glx 
        


<210>  69
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized variant OPH enzyme 

<400>  69

Met Ile Thr Asn Ser Gly Asp Arg Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Thr Ile Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Val Glu Lys Ala Val Arg Gly Leu Arg Arg Ala Arg Ala 
    50                  55                  60                  


Ala Gly Val Arg Thr Ile Val Asp Val Ser Thr Phe Asp Ile Gly Arg 
65                  70                  75                  80  


Asp Val Ser Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Phe Asp Pro Pro Leu Ser Met Arg Leu 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln Tyr 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Arg Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ala Ala 
                165                 170                 175     


Ser Gln Arg Gly Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Ala Leu Ala Ala Arg Gly Tyr Leu Ile Gly Leu 
    210                 215                 220                 


Asp His Ile Pro His Ser Ala Ile Gly Leu Glu Asp Asn Ala Ser Ala 
225                 230                 235                 240 


Ser Ala Leu Leu Gly Asn Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Gln Gly Tyr Met Lys Gln Ile Leu Val Ser Asn 
            260                 265                 270         


Asp Trp Leu Phe Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Ser Val Asn Pro Asp Gly Met Ala Phe Ile Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Gln Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Ile Thr Val Thr Asn Pro Ala Arg Phe Leu Ser Pro Thr Leu Arg Ala 
                325                 330                 335     


Ser Glx 
        


<210>  70
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized variant OPH enzyme 

<400>  70

Met Ile Thr Asn Ser Gly Asp Arg Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Thr Ile Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Val Glu Lys Ala Val Arg Gly Leu Arg Arg Ala Arg Ala 
    50                  55                  60                  


Ala Gly Val Arg Thr Ile Val Asp Val Ser Thr Phe Asp Ile Gly Arg 
65                  70                  75                  80  


Asp Val Ser Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Phe Asp Pro Pro Leu Ser Met Arg Leu 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln Tyr 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Arg Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ala Ala 
                165                 170                 175     


Ser Gln Arg Gly Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Ala Leu Ala Ala Arg Gly Tyr Leu Ile Gly Leu 
    210                 215                 220                 


Asp His Ile Pro Trp Ser Ala Ile Gly Leu Glu Asp Asn Ala Ser Ala 
225                 230                 235                 240 


Ser Ala Leu Leu Gly Asn Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Gln Gly Tyr Met Lys Gln Ile Leu Val Ser Asn 
            260                 265                 270         


Asp Trp Leu Phe Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Ser Val Asn Pro Asp Gly Met Ala Phe Ile Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Gln Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Ile Thr Val Thr Asn Pro Ala Arg Phe Leu Ser Pro Thr Leu Arg Ala 
                325                 330                 335     


Ser Glx 
        


<210>  71
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized variant OPH enzyme 

<400>  71

Met Ile Thr Asn Ser Gly Asp Arg Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Thr Ile Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Val Glu Lys Ala Val Arg Gly Leu Arg Arg Ala Arg Ala 
    50                  55                  60                  


Ala Gly Val Arg Thr Ile Val Asp Val Ser Thr Phe Asp Ile Gly Arg 
65                  70                  75                  80  


Asp Val Ser Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Asp Asp Pro Pro Leu Ser Met Arg Leu 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln Tyr 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Arg Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ala Ala 
                165                 170                 175     


Ser Gln Arg Gly Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Ala Leu Ala Ala Arg Gly Tyr Leu Ile Gly Leu 
    210                 215                 220                 


Asp His Ile Pro Trp Ser Ala Ile Gly Leu Glu Asp Asn Ala Ser Ala 
225                 230                 235                 240 


Ser Ala Leu Leu Gly Asn Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Gln Gly Tyr Met Lys Gln Ile Leu Val Ser Asn 
            260                 265                 270         


Asp Trp Leu Phe Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Ser Val Asn Pro Asp Gly Met Ala Phe Ile Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Gln Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Ile Thr Val Thr Asn Pro Ala Arg Phe Leu Ser Pro Thr Leu Arg Ala 
                325                 330                 335     


Ser Glx 
        


<210>  72
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized variant OPH enzyme 

<400>  72

Met Ile Thr Asn Ser Gly Asp Arg Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Thr Ile Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Val Glu Lys Ala Val Arg Gly Leu Arg Arg Ala Arg Ala 
    50                  55                  60                  


Ala Gly Val Arg Thr Ile Val Asp Val Ser Thr Phe Asp Ile Gly Arg 
65                  70                  75                  80  


Asp Val Ser Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Cys Asp Pro Pro Leu Ser Met Arg Leu 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln Tyr 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Arg Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ala Ala 
                165                 170                 175     


Ser Gln Arg Gly Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Ala Leu Ala Ala Arg Gly Tyr Leu Ile Gly Leu 
    210                 215                 220                 


Asp His Ile Pro Trp Ser Ala Ile Gly Leu Glu Asp Asn Ala Ser Ala 
225                 230                 235                 240 


Ser Ala Leu Leu Gly Asn Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Gln Gly Tyr Met Lys Gln Ile Leu Val Ser Asn 
            260                 265                 270         


Asp Trp Leu Phe Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Ser Val Asn Pro Asp Gly Met Ala Phe Ile Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Gln Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Ile Thr Val Thr Asn Pro Ala Arg Phe Leu Ser Pro Thr Leu Arg Ala 
                325                 330                 335     


Ser Glx 
        


<210>  73
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized variant OPH enzyme 

<400>  73

Met Ile Thr Asn Ser Gly Asp Arg Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Thr Ile Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Val Glu Lys Ala Val Arg Gly Leu Arg Arg Ala Arg Ala 
    50                  55                  60                  


Ala Gly Val Arg Thr Ile Val Asp Val Ser Thr Phe Asp Val Gly Arg 
65                  70                  75                  80  


Asp Val Ser Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Asp Asp Pro Pro Leu Ser Met Arg Leu 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln Tyr 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Arg Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ala Ala 
                165                 170                 175     


Ser Gln Arg Gly Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Ala Leu Ala Ala Arg Gly Tyr Leu Ile Gly Leu 
    210                 215                 220                 


Asp His Ile Pro Trp Ser Ala Ile Gly Leu Glu Asp Asn Ala Ser Ala 
225                 230                 235                 240 


Ser Ala Leu Leu Gly Asn Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Gln Gly Tyr Met Lys Gln Ile Leu Val Ser Asn 
            260                 265                 270         


Asp Trp Leu Phe Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Ser Val Asn Pro Asp Gly Met Ala Phe Ile Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Gln Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Ile Thr Val Thr Asn Pro Ala Arg Phe Leu Ser Pro Thr Leu Arg Ala 
                325                 330                 335     


Ser Glx 
        


<210>  74
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized variant OPH enzyme 

<400>  74

Met Ile Thr Asn Ser Gly Asp Arg Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Thr Ile Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Val 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Val Glu Lys Ala Val Arg Gly Leu Arg Arg Ala Arg Ala 
    50                  55                  60                  


Ala Gly Val Arg Thr Ile Val Asp Val Ser Thr Phe Asp Val Gly Arg 
65                  70                  75                  80  


Asp Val Ser Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Asp Asp Pro Pro Leu Ser Met Arg Leu 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln Tyr 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Arg Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ala Ala 
                165                 170                 175     


Ser Gln Arg Gly Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Ala Leu Ala Ala Arg Gly Tyr Leu Ile Gly Leu 
    210                 215                 220                 


Asp His Ile Pro Trp Ser Ala Ile Gly Leu Glu Asp Asn Ala Ser Ala 
225                 230                 235                 240 


Ser Ala Leu Leu Gly Asn Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Gln Gly Tyr Met Lys Gln Ile Leu Val Ser Asn 
            260                 265                 270         


Asp Trp Leu Phe Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Ser Val Asn Pro Asp Gly Met Ala Phe Ile Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Gln Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Ile Thr Val Thr Asn Pro Ala Arg Phe Leu Ser Pro Thr Leu Arg Ala 
                325                 330                 335     


Ser Glx 
        


<210>  75
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized variant OPH enzyme 

<400>  75

Met Ile Thr Asn Ser Gly Asp Arg Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Thr Ile Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Val 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Val Glu Lys Ala Val Arg Gly Leu Arg Arg Ala Arg Ala 
    50                  55                  60                  


Ala Gly Val Arg Thr Ile Val Asp Val Ser Thr Phe Asp Val Gly Arg 
65                  70                  75                  80  


Asp Val Ser Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Asp Asp Pro Pro Leu Ser Met Arg Leu 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln Tyr 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Arg Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ala Ala 
                165                 170                 175     


Ser Gln Arg Gly Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Ala Leu Ala Ala Arg Gly Tyr Leu Ile Gly Leu 
    210                 215                 220                 


Asp His Ile Pro Trp Ser Ala Ile Gly Leu Glu Asp Asn Ala Ser Ala 
225                 230                 235                 240 


Ser Ala Leu Leu Gly Asn Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Gln Gly Tyr Met Lys Gln Ile Leu Val Ser Asn 
            260                 265                 270         


Asp Trp Leu Phe Gly Val Ser Ser Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Ser Val Asn Pro Asp Gly Met Ala Phe Ile Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Gln Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Ile Thr Val Thr Asn Pro Ala Arg Phe Leu Ser Pro Thr Leu Arg Ala 
                325                 330                 335     


Ser Glx 
        


<210>  76
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized variant OPH enzyme 

<400>  76

Met Ile Thr Asn Ser Gly Asp Arg Ile Asn Thr Val Arg Gly Pro Ile 
1               5                   10                  15      


Thr Ile Ser Glu Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly 
            20                  25                  30          


Ser Ser Ala Gly Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg 
        35                  40                  45              


Lys Ala Leu Val Glu Lys Ala Val Arg Gly Leu Arg Arg Ala Arg Ala 
    50                  55                  60                  


Ala Gly Val Arg Thr Ile Val Asp Val Ser Thr Phe Asp Val Gly Arg 
65                  70                  75                  80  


Asp Val Ser Leu Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile 
                85                  90                  95      


Val Ala Ala Thr Gly Leu Trp Asp Asp Pro Pro Leu Ser Met Arg Leu 
            100                 105                 110         


Arg Ser Val Glu Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln Tyr 
        115                 120                 125             


Gly Ile Glu Asp Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr 
    130                 135                 140                 


Thr Gly Lys Ala Thr Pro Phe Gln Glu Leu Val Leu Arg Ala Ala Ala 
145                 150                 155                 160 


Arg Ala Ser Leu Ala Thr Gly Val Pro Val Thr Thr His Thr Ala Ala 
                165                 170                 175     


Ser Gln Arg Gly Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly 
            180                 185                 190         


Leu Ser Pro Ser Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp 
        195                 200                 205             


Leu Ser Tyr Leu Thr Ala Leu Ala Ala Arg Gly Tyr Leu Ile Gly Leu 
    210                 215                 220                 


Asp His Ile Pro Trp Ser Ala Ile Gly Leu Glu Asp Asn Ala Ser Ala 
225                 230                 235                 240 


Ser Ala Leu Leu Gly Asn Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile 
                245                 250                 255     


Lys Ala Leu Ile Asp Gln Gly Tyr Met Lys Gln Ile Leu Val Ser Asn 
            260                 265                 270         


Asp Trp Leu Phe Gly Phe Ser Leu Tyr Val Thr Asn Ile Met Asp Val 
        275                 280                 285             


Met Asp Ser Val Asn Pro Asp Gly Met Ala Phe Ile Pro Leu Arg Val 
    290                 295                 300                 


Ile Pro Phe Leu Arg Glu Lys Gly Val Pro Gln Glu Thr Leu Ala Gly 
305                 310                 315                 320 


Ile Thr Val Thr Asn Pro Ala Arg Phe Leu Ser Pro Thr Leu Arg Ala 
                325                 330                 335     


Ser Glx 
        


<210>  77
<211>  1014
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized codon-optimized S308L gene for 
       expression in S. cerevisiae 

<400>  77
atgataacaa atagtgggga cagaattaac acagtacgtg gccctataac catctcagaa       60

gctggtttca ctttgacaca tgaacatatt tgtggctcta gtgctggctt tctaagggct      120

tggccagagt ttttcggaag cagaaaagct ctggttgaga aggctgtgcg tggtctacgt      180

cgtgcaagag cagctggtgt aaggactatt gttgacgtct ctactttcga tgtgggtaga      240

gatgtttccc tgttagccga ggtgtcaagg gctgctgatg tccacattgt tgctgcaacc      300

ggtttgtggg atgatccgcc tctatctatg agacttagat ccgtagaaga acttactcaa      360

tttttcttaa gggaaattca atacggaata gaggacacag gtattagagc aggtattatc      420

aaagtcgcca ccacggggaa ggcaacacca tttcaagaat tagttctaag agctgcggcg      480

agggcatctt tagctaccgg cgtcccggtt acaactcaca ctgccgcaag ccaaagagga      540

ggtgaacaac aggcagcaat ttttgaatcg gaggggttaa gtccatcgag agtctgcatt      600

ggtcattcag acgatacaga tgatttatcc tatttgactg ccttggctgc tagaggatac      660

ttgatcggac ttgatcatat cccatggtca gccattggat tagaagataa tgcctccgct      720

tctgctcttc taggtaacag aagctggcaa accagggcat tgttgatcaa ggcccttatt      780

gaccaaggtt atatgaaaca gatattagta tcgaatgact ggttgtttgg ttttagttta      840

tatgtgacga acataatgga tgtaatggat tctgttaatc ccgacggtat ggcgttcatt      900

ccattgagag tgatcccttt tctgagggaa aaaggcgttc ctcaggaaac attggccggt      960

ataacggtta ccaatcccgc acgtttctta tcaccaactc tgagggcgtc ttaa           1014


<210>  78
<211>  916
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized NEOr gene and upstream promoter 

<400>  78
atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc       60

ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg      120

catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt      180

cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc      240

agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact      300

gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt      360

gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca      420

ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat      480

gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg      540

catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga      600

agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga      660

cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa      720

tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga      780

catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt      840

cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct      900

tgacgagttc ttctga                                                      916


<210>  79
<211>  653
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized GAPDH promoter sequence 

<400>  79
cgagtttatc attatcaata ctcgccattt caaagaatac gtaaataatt aatagtagtg       60

attttcctaa ctttatttag tcaaaaaatt agccttttaa ttctgctgta acccgtacat      120

gccaaaatag ggggcgggtt acacagaata tataacatcg taggtgtctg ggtgaacagt      180

ttattcctgg catccactaa atataatgga gcccgctttt taagctggca tccagaaaaa      240

aaaagaatcc cagcaccaaa atattgtttt cttcaccaac catcagttca taggtccatt      300

ctcttagcgc aactacagag aacaggggca caaacaggca aaaaacgggc acaacctcaa      360

tggagtgatg caacctgcct ggagtaaatg atgacacaag gcaattgacc cacgcatgta      420

tctatctcat tttcttacac cttctattac cttctgctct ctctgatttg gaaaaagctg      480

aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct acttgactaa taagtatata      540

aagacggtag gtattgattg taattctgta aatctatttc ttaaacttct taaattctac      600

ttttatagtt agtctttttt ttagttttaa aacaccaaga acttagtttc gaa             653


<210>  80
<211>  190
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized TcycI transcriptional terminator 

<400>  80
atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt       60

ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct      120

gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg      180

acgctcgaag                                                             190


<210>  81
<211>  592
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized 5' flanking ribosomal sequence 

<400>  81
atgagagtag caaacgtaag tctaaaggtt gttttatagt agttaggatg tagaaaatgt       60

attccgatag gccattttac atttggaggg acggttgaaa gtggacagag gaaaaggtgc      120

ggaaatggct gattttgatt gtttatgttt tgtgtgatga ttttacattt ttgcatagta      180

ttaggtagtc agatgaaaga tgaatagaca taggagtaag aaaacataga atagttaccg      240

ttattggtag gagtgtggtg gggtggtata gtccgcattg ggatgttact ttcctgttat      300

ggcatggatt tccctttagg gtctctgaag cgtatttccg tcaccgaaaa aggcagaaaa      360

agggaaactg aagggaggat agtagtaaag tttgaatggt ggtagtgtaa tgtatgatat      420

ccgttggttt tggtttcggt tgtgaaaagt tttttggtat gatattttgc aagtagcata      480

tatttcttgt gtgagaaagg tatattttgt atgttttgta tgttcccgcg cgtttccgta      540

ttttccgctt ccgcttccgc agtaaaaaat agtgaggaac tgggttaccc gg              592


<210>  82
<211>  663
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized 3' flanking ribosomal sequence 

<400>  82
ggcacctgtc actttggaaa aaaaatatac gctaagattt ttggagaata gcttaaattg       60

aagtttttct cggcgagaaa tacgtagtta aggcagagcg acagagaggg caaaagaaaa      120

taaaagtaag attttagttt gtaatgggag ggggggttta gtcatggagt acaagtgtga      180

ggaaaagtag ttgggaggta cttcatgcga aagcagttga agacaagttc gaaaagagtt      240

tggaaacgaa ttcgagtagg cttgtcgttc gttatgtttt tgtaaatggc ctcgtcaaac      300

ggtggagaga gtcgctaggt gatcgtcaga tctgcctagt ctctatacag cgtgtttaat      360

tgacatgggt tgatgcgtat tgagagatac aatttgggaa gaaattccca gagtgtgttt      420

cttttgcgtt taacctgaac agtctcatcg tgggcatctt gcgattccat tggtgagcag      480

cgaaggattt ggtggattac tagctaatag caatctattt caaagaattc aaacttgggg      540

gaatgccttg ttgaatagcc ggtcgcaaga ctgtgattct tcaagtgtaa cctcctctca      600

aatcagcgat atcaaacgta ccattccgtg aaacaccggg gtatctgttt ggtggaacct      660

gat                                                                    663


<210>  83
<211>  365
<212>  PRT
<213>  Flavobacterium sp.


<220>
<221>  misc_feature
<223>  Flavobacterium sp. parathion hydrolase gene

<400>  83

Met Gln Thr Arg Arg Val Val Leu Lys Ser Ala Ala Ala Ala Gly Thr 
1               5                   10                  15      


Leu Leu Gly Gly Leu Ala Gly Cys Ala Ser Val Ala Gly Ser Ile Gly 
            20                  25                  30          


Thr Gly Asp Arg Ile Asn Thr Val Arg Gly Pro Ile Thr Ile Ser Glu 
        35                  40                  45              


Ala Gly Phe Thr Leu Thr His Glu His Ile Cys Gly Ser Ser Ala Gly 
    50                  55                  60                  


Phe Leu Arg Ala Trp Pro Glu Phe Phe Gly Ser Arg Lys Ala Leu Ala 
65                  70                  75                  80  


Glu Lys Ala Val Arg Gly Leu Arg Arg Ala Arg Ala Ala Gly Val Arg 
                85                  90                  95      


Thr Ile Val Asp Val Ser Thr Phe Asp Ile Gly Arg Asp Val Ser Leu 
            100                 105                 110         


Leu Ala Glu Val Ser Arg Ala Ala Asp Val His Ile Val Ala Ala Thr 
        115                 120                 125             


Gly Leu Trp Phe Asp Pro Pro Leu Ser Met Arg Leu Arg Ser Val Glu 
    130                 135                 140                 


Glu Leu Thr Gln Phe Phe Leu Arg Glu Ile Gln Tyr Gly Ile Glu Asp 
145                 150                 155                 160 


Thr Gly Ile Arg Ala Gly Ile Ile Lys Val Ala Thr Thr Gly Lys Ala 
                165                 170                 175     


Thr Pro Phe Gln Glu Leu Val Leu Lys Ala Ala Ala Arg Ala Ser Leu 
            180                 185                 190         


Ala Thr Gly Val Pro Val Thr Thr His Thr Ala Ala Ser Gln Arg Asp 
        195                 200                 205             


Gly Glu Gln Gln Ala Ala Ile Phe Glu Ser Glu Gly Leu Ser Pro Ser 
    210                 215                 220                 


Arg Val Cys Ile Gly His Ser Asp Asp Thr Asp Asp Leu Ser Tyr Leu 
225                 230                 235                 240 


Thr Ala Leu Ala Ala Arg Gly Tyr Leu Ile Gly Leu Asp His Ile Pro 
                245                 250                 255     


His Ser Ala Ile Gly Leu Glu Asp Asn Ala Ser Ala Ser Ala Leu Leu 
            260                 265                 270         


Gly Ile Arg Ser Trp Gln Thr Arg Ala Leu Leu Ile Lys Ala Leu Ile 
        275                 280                 285             


Asp Gln Gly Tyr Met Lys Gln Ile Leu Val Ser Asn Asp Trp Leu Phe 
    290                 295                 300                 


Gly Phe Ser Ser Tyr Val Thr Asn Ile Met Asp Val Met Asp Arg Val 
305                 310                 315                 320 


Asn Pro Asp Gly Met Ala Phe Ile Pro Leu Arg Val Ile Pro Phe Leu 
                325                 330                 335     


Arg Glu Lys Gly Val Pro Gln Glu Thr Leu Ala Gly Ile Thr Val Thr 
            340                 345                 350         


Asn Pro Ala Arg Phe Leu Ser Pro Thr Leu Arg Ala Ser 
        355                 360                 365 


<210>  84
<211>  1693
<212>  DNA
<213>  Flavobacterium sp.


<220>
<221>  misc_feature
<223>  Flavobacterium sp. parathion hydrolase gene

<400>  84
ggatccgagc ggcttaccgg cgccctgctc gaccggttga cccaccatgt ccatatcctc       60

gagatgaacg ggggcagcta tcggcttgcc agcagccgga aacggcaaaa gcacagccac      120

cacgcacagc aagaggaggt gcccccccca tgacctgatc agaaaacccc tcatctgctg      180

tgctgaacgg ccttcgctac gctccagacc gttcagcaca gcagatgaaa gcaccgcctc      240

gacaagaggc tttttgttca atccaactgg tacactctta caccggaatc ttgcacaatt      300

ttaccccggc attgacatct gacgcgtcaa cagtaaaaga aacaaccggt tcagatctgc      360

agcctgactc ggcaccagtc gctgcaagca gagtcgtaag caatcgcaag ggggcagcat      420

gcaaacgaga agggttgtgc tcaagtctgc ggccgccgca ggaactctgc tcggcggcct      480

ggctgggtgc gcgagcgtgg ctggatcgat cggcacaggc gatcggatca ataccgtgcg      540

cggtcctatc acaatctctg aagcgggttt cacactgact cacgagcaca tctgcggcag      600

ctcggcagga ttcttgcgtg cttggccaga gttcttcggt agccgcaaag ctctagcgga      660

aaaggctgtg agaggattgc gccgcgccag agcggctggc gtgcgaacga ttgtcgatgt      720

gtcgactttc gatatcggtc gcgacgtcag tttattggcc gaggtttcgc gggctgccga      780

cgttcatatc gtggcggcga ccggcttgtg gttcgacccg ccactttcga tgcgattgag      840

gagtgtagag gaactcacac agttcttcct gcgtgagatt caatatggca tcgaagacac      900

cggaattagg gcgggcatta tcaaggtcgc gaccacaggc aaggcgaccc cctttcagga      960

gttagtgtta aaggcggccg cccgggccag cttggccacc ggtgttccgg taaccactca     1020

cacggcagca agtcagcgcg atggtgagca gcaggccgcc atttttgagt ccgaaggctt     1080

gagcccctca cgggtttgta ttggtcacag cgatgatact gacgatttga gctatctcac     1140

cgccctcgct gcgcgcggat acctcatcgg tctagaccac atcccgcaca gtgcgattgg     1200

tctagaagat aatgcgagtg catcagccct cctgggcatc cgttcgtggc aaacacgggc     1260

tctcttgatc aaggcgctca tcgaccaagg ctacatgaaa caaatcctcg tttcgaatga     1320

ctggctgttc gggttttcga gctatgtcac caacatcatg gacgtgatgg atcgcgtgaa     1380

ccccgacggg atggccttca ttccactgag agtgatccca ttcctacgag agaagggcgt     1440

cccacaggaa acgctggcag gcatcactgt gactaacccg gcgcggttct tgtcaccgac     1500

cttgcgggcg tcatgacgcc atctggatcc ttccagccag cggccactat tccccgtcaa     1560

gataccgaac gatgaagtcg cgcatcgatc gataggcatc ttcaatttga tcagggctgc     1620

cacctccaaa gccgtggcca cccctgtcga tagtcttgag gacgtagggc acaccgtgct     1680

tttcgaactg cag                                                        1693


<210>  85
<211>  351
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  Accession No. YGR035C  Chr 7

<400>  85
atgttactca caccagccaa gactacaaga acggaagact cagcaaactc aacagatgac       60

agcagtaaaa gcagtaattc attcatgcgt gccatagttt cttcccttat ggttaagccc      120

ataacatctc tgactaatac agtcacatgc agacaaagtt cccatcacaa tagctctcca      180

agtaaaatca caagatatga tttaatcaaa gctgctgctg aaaatgactt gaaaaggagt      240

aagtctcagg gacgagaaaa atccagaaga aactctaatc gtagaaataa tgaagaaatt      300

tttgtggcca atactgcttc agaaatccaa cggactaaaa gtagcatata g               351


<210>  86
<211>  981
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  Accession No. YHR139C  Chr 8

<400>  86
atgaaattca catcagtgct agcatttttt cttgcaactt taacagcttc tgcaacacca       60

ctttacaaga ggcagaacgt tacttctggc ggcggtacgg tccccgtgat catcacgggt      120

ggacctgctg tatctggtag ccagtcaaac gttactacca caacgctatt caactctact      180

tccaccttaa acatcactca actttaccaa attgctactc aagttaatca gactttacaa      240

agcgaatcgt cttccggcat tataatcgtc acaaactggc gttccattga aactttgagt      300

ttcttctgct caatcgtttt taatacatca aagaccattg tcattactga gaatttctta      360

tggggtgtac caatattgag tagttcggac gcggaaggta gaggcacttt ggttgccggt      420

agagataaag ttgtgtattc tggtgttttc cctccttaca ctgttcctgt gggcgttttg      480

tctggtcaga agaatgttca atggttcttt gatgcctgtg agccaactct gattgccagc      540

aactccacaa tcagaactca atactcgaat tttacgagcg cacaaatatc ttctaatgcc      600

tcctccggga ccaatacatc ctcatcatcc tccagtccat tggttccaat tatatacgaa      660

gaaggatatt ctcaaagctt aattcaatca ctaagttcta gtatccaagg tttggttgtt      720

gtcagctctg gtacaagtca caacagtacc gtggcatcat ggacatcggt cgacttccct      780

gtcgtttatg caagtgatgg atccagtggt catgatggtt caggaattgg gttcataagt      840

aacacaagta ttccacaagg tgcaatttct gctgggtact tgtcaccaat ccaagcgcaa      900

actttgttgt ctattgctat ccataatcaa gtcacaagta gtagcgagtt acaacaaatc      960

ttccctgtta gtcaacaata a                                                981


<210>  87
<211>  435
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  Accession No. YOR186W  Chr 15

<400>  87
atgactttgg cttttacgac cttcgctatt tccaagataa acaattcgtc cacgaatgaa       60

gattctaaag tcatgatact gtgcgatgag catcaccctt tcgagaaggg ttactttaag      120

tccgccatac gagccttcgg caacagcatt aagctaggat taatgggtaa ctcgcgacca      180

gaagacgcag cgtccatatt ccaagataaa aatattcctc acgatctgac gacggaagaa      240

ttcaggttgc agctggtctg tatggctttt tcctggttca tatttggtct cttcatcgcc      300

tgcttactac tttgtatcac gcttgtgcta acatcgcgat atcctggaga aaatgaaaac      360

aaagccacgg aagttgtgcc atccagtaat attgacgacg aagaaaagca attatcgctt      420

tccgacatga tttag                                                       435


<210>  88
<211>  954
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  Accession No. YGR213C  Chr 7

<400>  88
atggcaaaag acggcttcga gttatataga tacactccgg aattaggtgc gagtatactt       60

tttacggtac tttttgcagt atcaggggta gcatttgtaa ttttactatt ccattattct      120

gtgaaaagta aaaggagagt tggctctctt atgaagtccc aacccgtatt gcggtattac      180

ggtacggtaa acttagcagg agcgtacatt ccctttatat ttgggtgttt tgttgagtgt      240

gtgggctttg cgtttagatg taaatcaagc aaagacacca cactactaaa cccctatatt      300

attcaaacgg ttttcttgct ggtgtctcca acattatatg ctgccagtat ttacatgatt      360

tttggtagaa tggctactct gctgttcgca gaaaacttaa tgatcatgcc cgctagattc      420

aatactacaa tatttgtgat tggcgatgta ggcagtcttc tattacaagc aattggtggt      480

gctatgatgt ctaaagtgac aagtgcatct tctggttctc atctcgttac agctggtttg      540

ttcattcaaa ttgctttttt tggtctcttc attataaatg aggttttgtt cattttcaag      600

atgagcaaaa aaccaaccaa tgtatcggta aggtatggta gttggaaata cctaaacatt      660

gctttattgg tgaacagttt cctgatttta attaggtcga tcgtcagagc tgttgaattt      720

attcagggtt atgacggaga aattgcctcg cacgagtggt atctttatat ttttgatggt      780

ttaccaatgt ttttactcgt attaattttt attgtggcgt tcccactaat taacattttt      840

agaatacatg aagagtctat tcaagcgcaa caatcagcaa gatttgacgg caccgactat      900

cctgacgttg aggtgacgtc tatcgaagaa gatctcgctt caaagagcga ataa            954


<210>  89
<211>  306
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  Accession No. YLR346C  Chr 12

<400>  89
atgcaatcga tcagtaattg tcccatcggg ttagtttcaa aaaacacaat caattcagct       60

tccactattg cagagtgggt agcatgtcca tggaaatata tcaacgttgt tggttcaggc      120

agatatgtga gcaataaacc tgataaaatt accagatatg atttactcaa ggctgcccag      180

gaagcggaaa tgcaggagtt gcttacaaga aatgatatga aaggtagaca taaacgtaat      240

aagaaaagta agatagcatt ggagactata gcggaagaaa actcttcaac tgaaagcctt      300

ttttaa                                                                 306


<210>  90
<211>  564
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  Accession No. YIR017C  Chr 9

<400>  90
atgagtgcga aacaagggtg ggaaaagaaa tcaacaaaca ttgacattgc aagcaggaag       60

ggtatgaacg tgaacaatct ttcggaacat ctacagaacc tgatatcaag cgattcagag      120

ctgggctcgc gcttgctatc attgctgctg gttagcagtg gcaacgcgga ggagctcatt      180

agcatgataa ataatggcca ggatgtctcg cagtttaaga agctccgcga gccgagaaag      240

gggaaggttg ccgcaacaac agcagttgtt gtaaaagaag aggaagctcc cgtgagcact      300

tctaacgaac tggataagat taagcaagag cggagaagaa agaacacaga ggcgtcgcag      360

cggttccgca ttcgcaagaa acaaaagaat tttgagaaca tgaataagct gcagaactta      420

aacacgcaaa tcaacaaact tcgcgatcgt atagagcaat tgaacaagga aaatgagttt      480

tggaaagcaa agctcaatga cattaacgag attaagtctt tgaaattgtt gaatgacatt      540

aagagacgga acatgggcag gtag                                             564


<210>  91
<211>  897
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  Accession No. YLL056C  Chr 12

<400>  91
atgaaagtat ttataactgg tgcttctggc ttcattggtt ctgccgtatt atctgaacta       60

atatcctcgg gtcatgaagt tgttggcttg gcaaggtcag acgaggctgc tgcaaagatt      120

aagtcgattg atcctgctgc aaaaatcctt cgcggtgatc tcaaggatct ggaaattttg      180

aaaaaaggtg ccactgaatc tgatggcgtt attcatttag gatttgtgca tgattttaaa      240

aattttgaac agtgctgcga aattgaccgt caggccactg tagctatgct agagtcactt      300

aaaggttcta acaagccttt cttatatacg aatggtacac tttctttgcg accaaacaaa      360

gttgctaacg aacaggatgg tattgatgaa gattccaaaa ttttgcgtgc tgtcactgaa      420

caggttgcct tgagttacaa agacaaaggt gtttcagcaa gaattgtcag actcccattc      480

tcggttcatg gcaaggggga caaggctttt gtaccaatat taatgaatat tgccaaagct      540

gccggaaaat ctggctatgt cggacaaggc acaaacgctt gggcggctgt acatcgtttg      600

gatacggctc ctctgttcag acttgtttta gagaaaggaa aaacaggaca agtgtatcat      660

tgcgttggtg aacaaggtat accattcaaa gatattgcgc gtgtgattgg agaaattttg      720

aatgttcccg tggcctctat ccctgttgat gacgcggaaa gtcattttgg cttcctcact      780

tgttttgtca ctagagatgg cccagtttca agcgaaggta ccagaaaaga gctgggatgg      840

cagccacaac aaatcggtct tcttgaagat atccgtgcga actatagctt aaactga         897


<210>  92
<211>  2247
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  Accession No. YGL205W  Chr 7

<400>  92
atgacgagac gtactactat taatcccgat tcggtggttc tgaatcctca aaaatttatc       60

cagaaagaaa gggcggattc gaaaatcaaa gttgaccaag ttaacacatt tttagagtca      120

tccccggaga ggagaactct gacgcacgcc ttaatagacc aaatagtgaa tgatcctata      180

ttgaaaactg atacggacta ttacgatgct aaaaaaatgc aagagagaga aattactgcc      240

aaaaaaatag ctaggcttgc tagttatatg gagcacgata tcaaaacagt gcgcaaacac      300

tttcgcgaca ctgacctgat gaaagagttg caagcaaatg atccagacaa agcttcgcct      360

ttaacaaaca aagacctttt tatattcgat aagagattgt cacttgtagc aaatattgat      420

cctcaattgg gtacgcgcgt gggtgtacac ttggggctat ttggtaattg tatcaagggc      480

aatggtactg atgagcaaat ccggtattgg ttgcaggaga gaggtgccac tttgatgaaa      540

ggtatatatg gctgttttgc aatgactgag ttaggacatg gttccaatgt tgcccagctg      600

cagactaggg ctgtgtacga taagcaaaat gatacttttg taattgatac acctgatcta      660

actgccacca aatggtggat tggtggggct gcccattctg ccacgcacgc tgccgtgtac      720

gccagattga tcgttgaagg taaagactac ggtgtaaaaa cattcgttgt tcctctgaga      780

gacccttcga ctttccaact gttagctggt gtttccatag gggatattgg agcgaagatg      840

ggtcgtgacg gtattgataa tggctggatc cagttcagaa acgtagttat ccctagagaa      900

tttatgctaa gtagatttac caaagttgtc cgttctccag atggttcagt caccgtcaaa      960

actgagccac aattggatca aatttctggt tatagtgcat tgttaagtgg tagagttaac     1020

atggtcatgg attcatttag gtttggctcc aaatttgcta ctattgctgt acgttacgcg     1080

gttggtcgtc agcaattcgc acctagaaag ggattgtctg aaacacaatt aatcgactat     1140

ccccttcacc aatatcgtgt tttaccacaa ttgtgtgttc catatttggt gtcacctgta     1200

gcttttaagt taatggacaa ctattattcc actttggacg agttatacaa cgcttcctca     1260

tctgcataca aagctgctct ggttaccgtg agtaaaaagt tgaagaattt atttattgat     1320

agcgccagct tgaaagccac caatacttgg ttaattgcta cactgattga tgagttgaga     1380

cagacttgcg gaggacatgg gtattcacag tataacggat ttggtaaagg ctatgacgac     1440

tgggtggttc agtgcacatg ggagggtgat aataatgttt tatctttaac ttcagcaaaa     1500

tcaatattga aaaaatttat cgattcagcc acaaagggta gatttgacaa cacactggat     1560

gtggactcat tctcttactt aaaacctcag tacataggat ctgtggtttc tggagaaata     1620

aagagtggtt taaaggagtt gggtgattat actgaaattt ggtctatcac cttaatcaaa     1680

ttactggcac atattggtac tttagttgaa aaatcaagaa gtattgatag cgtttctaag     1740

cttttagtct tagtatccaa atttcatgcc ttgcgctgca tgttgaaaac ctattacgac     1800

aagttaaact ctcgtgattc acatatttcc gatgaaatta caaaggaatc tatgtggaat     1860

gtttataagt tattttcctt gtattttatt gacaagcatt ccggagaatt ccaacaattc     1920

aagatcttca ctcctgatca gatctctaaa gttgtgcagc cacaactatt ggctcttttg     1980

ccaattgtga ggaaagactg tataggtctg acagactcct ttgaattacc tgacgcgatg     2040

ttaaattctc ctataggtta ctttgatggc gatatctatc acaattactt caatgaagtt     2100

tgccgcaata atccagtgga ggcagatggg gcagggaagc cttcttatca tgcgctgttg     2160

agcagcatgc tcggtagagg tttcgaattt gaccaaaagt taggtggtgc agctaatgcg     2220

gaaattttat cgaaaataaa caagtga                                         2247


<210>  93
<211>  1704
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  Accession No. YJL219W  Chr 10

<400>  93
atgtccggtg ttaataatac atccgcaaat gatttatcca ctaccgagtc taactctaac       60

tcagtagcaa atgcaccatc tgtaaaaact gagcataatg actctaaaaa ctccctcaac      120

ctggatgcca ctgaaccacc tattgactta cctcaaaaac ccctctctgc atataccacc      180

gtcgcaatcc tgtgtttgat gattgcattt ggcggcttca tctttggttg ggataccggt      240

accatttctg gttttgttaa cctttctgat ttcatcagaa ggttcggtca aaaaaatgac      300

aagggaacct actacttatc gaaagtaaga atgggtttga tcgtctcaat attcaacatt      360

ggctgcgcca taggcggaat tgtcttgtca aaagtcggtg atatatatgg tcgtcgtatt      420

ggattgatta cagttactgc catttacgtt gtaggcatcc taatccaaat aacttccata      480

aacaagtggt accaatactt cattggaaga attatttctg gcctaggagt gggaggcatt      540

gctgtccttt ccccaatgtt gatatctgaa gttgctccca aacaaatcag aggaaccctg      600

gtccaattgt accagctgat gtgtacgatg ggtatttttc taggatactg taccaattac      660

ggtaccaaga actatcacaa cgccactcaa tggagagtcg gccttggtct ttgctttgcc      720

tggactacat tcatggttag tggaatgatg tttgtaccag aatcaccacg ttacctgatt      780

gaggttggta aagatgagga agcgaaacgt tcactttcga aatccaacaa agtctcagtc      840

gacgatccag ccttgttagc agaatatgac actataaagg cgggaatcga acttgaaaag      900

ctggcaggta acgcatcatg gtctgaacta ctctccacta aaacaaaggt ctttcagcgt      960

gttctcatgg gagtgatgat ccaatcgctg cagcaattaa ccggtgataa ctacttcttt     1020

tactacggca ccaccatctt caaatctgtc ggtctaaagg actcctttca gacttcgatc     1080

attatcggtg tggttaattt tttctcttca ttcatagcgg tatacaccat tgagaggttt     1140

ggacgccgta cgtgtctatt gtggggtgct gcttctatgc tatgctgctt tgctgtgttt     1200

gcctccgtcg gtgtgacaaa gttgtggcct caaggaagca gtcaccaaga cattacttct     1260

cagggcgccg gtaactgtat gattgtgttt actatgttct tcattttttc gttcgccacc     1320

acttgggcag gcggctgtta cgttattgtc tcagagacgt ttcctcttag ggtcaaatca     1380

agaggaatgg caatcgcaac agctgcaaac tggatgtggg gtttcctgat tagtttcttt     1440

accccattca ttaccggggc aatcaacttt tactacggtt atgtattctt aggctgtctg     1500

gtttttgcat acttttatgt ctttttcttt gtcccagaaa caaaaggcct gacgctggag     1560

gaggtgaata ctatgtggct ggaaggtgtg ccagcatgga aatcagcctc atgggtgcca     1620

ccagaaagaa gaaccgcaga ttacgatgct gacgccatag accatgacga tagaccaatc     1680

tacaagaggt tcttttccag ctaa                                            1704


<210>  94
<211>  1770
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  Accession No. YGR287C  Chr 7

<400>  94
atgactattt cttctgcaca tccagagaca gaaccaaagt ggtggaaaga ggccacgttc       60

tatcaaattt acccagcaag tttcaaagac tctaatgacg atggctgggg tgacatgaaa      120

gggattgcct ccaagctgga gtacatcaaa gagcttggtg ccgatgccat ttggatctca      180

ccattctacg actcgccaca agatgatatg ggttacgata ttgccaacta cgaaaaggtc      240

tggccaacat atggtacgaa tgaagactgc tttgccttga tcgaaaagac acataagctt      300

ggtatgaaat ttatcaccga cttggtcatc aatcactgtt ccagcgaaca tgaatggttc      360

aaagagagca gatcctcgaa gactaatcca aagcgtgact ggttcttctg gagacctcct      420

aaaggttatg acgccgaagg caagccaatt cctccaaaca attggaaatc ctattttggt      480

ggttccgcat ggaccttcga tgaaaagaca caagaattct acttgcgttt gttttgctcc      540

actcaacctg atttgaattg ggagaatgaa gactgtagaa aggcaatcta cgaaagtgcc      600

gttggatact ggttagacca tggtgtagac ggctttagaa ttgatgtcgg aagtttgtac      660

tccaaagttg taggtttacc agatgctcct gttgttgaca aaaactcgac ttggcaatcc      720

agtgatccat acacattgaa tggaccacgt attcacgagt tccatcaaga aatgaatcaa      780

ttcatcagaa acagagtgaa ggatggcagg gagattatga cagttggtga aatgcaacat      840

gcctccgacg aaactaagag actttatacg agtgcttcaa gacacgaact tagtgagtta      900

tttaactttt cccacactga tgtggggact tcacctttgt tccgttacaa cttggtccca      960

tttgaactga aggattggaa gattgccctt gctgagctgt tcaggtacat taatggtaca     1020

gattgttggt caacaatcta tctggaaaat cacgaccaac ctcgttcaat tacgagattt     1080

ggtgacgatt ctcccaagaa ccgtgttatt tctggtaagt tactctctgt gttgctaagt     1140

gccttgaccg gtactctata tgtgtatcag ggacaagagc ttggccaaat caatttcaag     1200

aactggcctg ttgaaaagta cgaggatgtc gaaatcagaa acaactacaa tgccattaaa     1260

gaagagcatg gggaaaactc agaggagatg aaaaagtttt tagaagccat tgcccttatc     1320

tccagggacc atgctagaac acctatgcaa tggtctcgtg aggagccaaa tgctggtttt     1380

tctggtccta gtgctaaacc atggttttac ttgaacgact ctttcagaga aggcattaac     1440

gtcgaagatg aaatcaagga tcccaactcg gttttgaact tctggaagga ggccttgaag     1500

tttagaaagg cgcataaaga cattactgtg tacggatacg atttcgagtt tattgattta     1560

gacaataaga agttgtttag cttcacaaag aagtacaaca ataaaacatt gtttgcggct     1620

ttgaacttta gctctgatgc gacagatttc aagattccaa atgatgattc atcgttcaag     1680

ttagagtttg gaaactatcc aaagaaggag gtagatgcct cttccagaac attgaagcca     1740

tgggaaggaa gaatatatat cagcgaatga                                      1770


<210>  95
<211>  1482
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  misc_feature
<223>  Accession No. YHL012W  Chr 8

<400>  95
atgacggttt tctctggagt aaataaaata gagtttgaag gtactttcga aggtattgga       60

aaggatgttg ttatgtcaca aatgataagg gccttgcaga aacattttcc ttcgatacgt      120

gacaagaatt atgagtttag cctatttttg cacatttttc aaagatatgt cttagaaaat      180

acttcaatca cccatgatct agtttgtgac aaaattagat taccgataat tgatgaagtg      240

gttgaactcg atgatattaa aaattacggt ttgttagagg gtaaactatt atcaaagtta      300

gctattttaa aattaaccgg aaaggctaat ccaatcattg gtaaagaaag ccctttattc      360

gaagtaaaaa atgggatgtc ttcattggat gtaatagttc gacaaacgca aaatttgaat      420

gtacgatata attcagacgt tcctcttata tttatgacct cgcttgaaac tgaatctcaa      480

gtatcaaact tccttgaaga gcattattct tctagcaaag tgagatggaa aaccgtagtt      540

cagtcaagct ttccacaaat tgataaagat agattattac ctatcgattt acaaataaat      600

tctcatgaaa atgatttttg gtatccttgc gggactggta acctaactga cactttgtat      660

ttttcaggag aattggacaa attaattgct caaggaaaag agattttatt tgtttcaaat      720

gttgacaatt tgggcgctac aggagatttg aacattttaa atttcatcat aaatgaaaaa      780

atcgagtatc ttgttgaagt agttgaaagg actgcaaatg tttcaaacac tggagtgttg      840

gcaacatata aaggtaaact acgatcggtt tattacaact gcttatccaa tgaaagtgca      900

agcacgtgtc gaattgtcaa cactaataac atttggattg atttgaaaaa gctaaaggtg      960

ctaatagagt ctaatagttt aaatttgcca attcattcta gtgaaagtaa aataacacac     1020

aaaaatgaag agatagagtg tcttcaattt aagacacagt tggtcgattg tattgccttc     1080

tttccgaaca gtcgagtatt gaaagtatca agagacagat ttttacccct aagaacatgt     1140

aaggacttgt ttttgttgaa atcaacatta tatgatctcg attcaaatgg aacatttaac     1200

ctctaccctc tgaaatttgg attattacct tcaatcgact tgggcgatga gtttgcaact     1260

tatgagactt ttaagatagg agtaccagat attcctaata ttctggaatt agagcattta     1320

actgtgatgg gaaatgtgtt ttttggtcgt aatataactc tgaaagggac ggtcattata     1380

atatgtgatg aaaatgatgt aattactgtt cccgatggct ctattttgga aaatgtaacg     1440

atttggcata aatctcagct ggaggatatg aatggatatt aa                        1482


