                         SEQUENCE LISTING

<110>  CYTOMICS SYSTEMS
 
<120>  Procd de criblage d'agents modulants l'activit 
       ubiquitine-ligase de Mdm2 et moyens destins  la mise en oeuvre 
       dudit procd

<130>  V570FR - CYTOMICS

<160>  12    

<170>  PatentIn version 3.3

<210>  1
<211>  491
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Cys Asn Thr Asn Met Ser Val Pro Thr Asp Gly Ala Val Thr Thr 
1               5                   10                  15      


Ser Gln Ile Pro Ala Ser Glu Gln Glu Thr Leu Val Arg Pro Lys Pro 
            20                  25                  30          


Leu Leu Leu Lys Leu Leu Lys Ser Val Gly Ala Gln Lys Asp Thr Tyr 
        35                  40                  45              


Thr Met Lys Glu Val Leu Phe Tyr Leu Gly Gln Tyr Ile Met Thr Lys 
    50                  55                  60                  


Arg Leu Tyr Asp Glu Lys Gln Gln His Ile Val Tyr Cys Ser Asn Asp 
65                  70                  75                  80  


Leu Leu Gly Asp Leu Phe Gly Val Pro Ser Phe Ser Val Lys Glu His 
                85                  90                  95      


Arg Lys Ile Tyr Thr Met Ile Tyr Arg Asn Leu Val Val Val Asn Gln 
            100                 105                 110         


Gln Glu Ser Ser Asp Ser Gly Thr Ser Val Ser Glu Asn Arg Cys His 
        115                 120                 125             


Leu Glu Gly Gly Ser Asp Gln Lys Asp Leu Val Gln Glu Leu Gln Glu 
    130                 135                 140                 


Glu Lys Pro Ser Ser Ser His Leu Val Ser Arg Pro Ser Thr Ser Ser 
145                 150                 155                 160 


Arg Arg Arg Ala Ile Ser Glu Thr Glu Glu Asn Ser Asp Glu Leu Ser 
                165                 170                 175     


Gly Glu Arg Gln Arg Lys Arg His Lys Ser Asp Ser Ile Ser Leu Ser 
            180                 185                 190         


Phe Asp Glu Ser Leu Ala Leu Cys Val Ile Arg Glu Ile Cys Cys Glu 
        195                 200                 205             


Arg Ser Ser Ser Ser Glu Ser Thr Gly Thr Pro Ser Asn Pro Asp Leu 
    210                 215                 220                 


Asp Ala Gly Val Ser Glu His Ser Gly Asp Trp Leu Asp Gln Asp Ser 
225                 230                 235                 240 


Val Ser Asp Gln Phe Ser Val Glu Phe Glu Val Glu Ser Leu Asp Ser 
                245                 250                 255     


Glu Asp Tyr Ser Leu Ser Glu Glu Gly Gln Glu Leu Ser Asp Glu Asp 
            260                 265                 270         


Asp Glu Val Tyr Gln Val Thr Val Tyr Gln Ala Gly Glu Ser Asp Thr 
        275                 280                 285             


Asp Ser Phe Glu Glu Asp Pro Glu Ile Ser Leu Ala Asp Tyr Trp Lys 
    290                 295                 300                 


Cys Thr Ser Cys Asn Glu Met Asn Pro Pro Leu Pro Ser His Cys Asn 
305                 310                 315                 320 


Arg Cys Trp Ala Leu Arg Glu Asn Trp Leu Pro Glu Asp Lys Gly Lys 
                325                 330                 335     


Asp Lys Gly Glu Ile Ser Glu Lys Ala Lys Leu Glu Asn Ser Thr Gln 
            340                 345                 350         


Ala Glu Glu Gly Phe Asp Val Pro Asp Cys Lys Lys Thr Ile Val Asn 
        355                 360                 365             


Asp Ser Arg Glu Ser Cys Val Glu Glu Asn Asp Asp Lys Ile Thr Gln 
    370                 375                 380                 


Ala Ser Gln Ser Gln Glu Ser Glu Asp Tyr Ser Gln Pro Ser Thr Ser 
385                 390                 395                 400 


Ser Ser Ile Ile Tyr Ser Ser Gln Glu Asp Val Lys Glu Phe Glu Arg 
                405                 410                 415     


Glu Glu Thr Gln Asp Lys Glu Glu Ser Val Glu Ser Ser Leu Pro Leu 
            420                 425                 430         


Asn Ala Ile Glu Pro Cys Val Ile Cys Gln Gly Arg Pro Lys Asn Gly 
        435                 440                 445             


Cys Ile Val His Gly Lys Thr Gly His Leu Met Ala Cys Phe Thr Cys 
    450                 455                 460                 


Ala Lys Lys Leu Lys Lys Arg Asn Lys Pro Cys Pro Val Cys Arg Gln 
465                 470                 475                 480 


Pro Ile Gln Met Ile Val Leu Thr Tyr Phe Pro 
                485                 490     


<210>  2
<211>  719
<212>  PRT
<213>  squence artificielle : code une protine de fusion

<400>  2

Met Val Ser Val Ile Lys Pro Glu Met Lys Ile Lys Leu Cys Met Arg 
1               5                   10                  15      


Gly Thr Val Asn Gly His Asn Phe Val Ile Glu Gly Glu Gly Lys Gly 
            20                  25                  30          


Asn Pro Tyr Glu Gly Thr Gln Ile Leu Asp Leu Asn Val Thr Glu Gly 
        35                  40                  45              


Ala Pro Leu Pro Phe Ala Tyr Asp Ile Leu Thr Thr Val Phe Gln Tyr 
    50                  55                  60                  


Gly Asn Arg Ala Phe Thr Lys Tyr Pro Ala Asp Ile Gln Asp Tyr Phe 
65                  70                  75                  80  


Lys Gln Thr Phe Pro Glu Gly Tyr His Trp Glu Arg Ser Met Thr Tyr 
                85                  90                  95      


Glu Asp Gln Gly Ile Cys Thr Ala Thr Ser Asn Ile Ser Met Arg Gly 
            100                 105                 110         


Asp Cys Phe Phe Tyr Asp Ile Arg Phe Asp Gly Thr Asn Phe Pro Pro 
        115                 120                 125             


Asn Gly Pro Val Met Gln Lys Lys Thr Leu Lys Trp Glu Pro Ser Thr 
    130                 135                 140                 


Glu Lys Met Tyr Val Glu Asp Gly Val Leu Lys Gly Asp Val Asn Met 
145                 150                 155                 160 


Arg Leu Leu Leu Glu Gly Gly Gly His Tyr Arg Cys Asp Phe Lys Thr 
                165                 170                 175     


Thr Tyr Lys Ala Lys Lys Glu Val Arg Leu Pro Asp Ala His Lys Ile 
            180                 185                 190         


Asp His Arg Ile Glu Ile Leu Lys His Asp Lys Asp Tyr Asn Lys Val 
        195                 200                 205             


Lys Leu Tyr Glu Asn Ala Val Ala Arg Tyr Ser Met Leu Pro Ser Gln 
    210                 215                 220                 


Ala Lys Glu Phe Met Cys Asn Thr Asn Met Ser Val Pro Thr Asp Gly 
225                 230                 235                 240 


Ala Val Thr Thr Ser Gln Ile Pro Ala Ser Glu Gln Glu Thr Leu Val 
                245                 250                 255     


Arg Pro Lys Pro Leu Leu Leu Lys Leu Leu Lys Ser Val Gly Ala Gln 
            260                 265                 270         


Lys Asp Thr Tyr Thr Met Lys Glu Val Leu Phe Tyr Leu Gly Gln Tyr 
        275                 280                 285             


Ile Met Thr Lys Arg Leu Tyr Asp Glu Lys Gln Gln His Ile Val Tyr 
    290                 295                 300                 


Cys Ser Asn Asp Leu Leu Gly Asp Leu Phe Gly Val Pro Ser Phe Ser 
305                 310                 315                 320 


Val Lys Glu His Arg Lys Ile Tyr Thr Met Ile Tyr Arg Asn Leu Val 
                325                 330                 335     


Val Val Asn Gln Gln Glu Ser Ser Asp Ser Gly Thr Ser Val Ser Glu 
            340                 345                 350         


Asn Arg Cys His Leu Glu Gly Gly Ser Asp Gln Lys Asp Leu Val Gln 
        355                 360                 365             


Glu Leu Gln Glu Glu Lys Pro Ser Ser Ser His Leu Val Ser Arg Pro 
    370                 375                 380                 


Ser Thr Ser Ser Arg Arg Arg Ala Ile Ser Glu Thr Glu Glu Asn Ser 
385                 390                 395                 400 


Asp Glu Leu Ser Gly Glu Arg Gln Arg Lys Arg His Lys Ser Asp Ser 
                405                 410                 415     


Ile Ser Leu Ser Phe Asp Glu Ser Leu Ala Leu Cys Val Ile Arg Glu 
            420                 425                 430         


Ile Cys Cys Glu Arg Ser Ser Ser Ser Glu Ser Thr Gly Thr Pro Ser 
        435                 440                 445             


Asn Pro Asp Leu Asp Ala Gly Val Ser Glu His Ser Gly Asp Trp Leu 
    450                 455                 460                 


Asp Gln Asp Ser Val Ser Asp Gln Phe Ser Val Glu Phe Glu Val Glu 
465                 470                 475                 480 


Ser Leu Asp Ser Glu Asp Tyr Ser Leu Ser Glu Glu Gly Gln Glu Leu 
                485                 490                 495     


Ser Asp Glu Asp Asp Glu Val Tyr Gln Val Thr Val Tyr Gln Ala Gly 
            500                 505                 510         


Glu Ser Asp Thr Asp Ser Phe Glu Glu Asp Pro Glu Ile Ser Leu Ala 
        515                 520                 525             


Asp Tyr Trp Lys Cys Thr Ser Cys Asn Glu Met Asn Pro Pro Leu Pro 
    530                 535                 540                 


Ser His Cys Asn Arg Cys Trp Ala Leu Arg Glu Asn Trp Leu Pro Glu 
545                 550                 555                 560 


Asp Lys Gly Lys Asp Lys Gly Glu Ile Ser Glu Lys Ala Lys Leu Glu 
                565                 570                 575     


Asn Ser Thr Gln Ala Glu Glu Gly Phe Asp Val Pro Asp Cys Lys Lys 
            580                 585                 590         


Thr Ile Val Asn Asp Ser Arg Glu Ser Cys Val Glu Glu Asn Asp Asp 
        595                 600                 605             


Lys Ile Thr Gln Ala Ser Gln Ser Gln Glu Ser Glu Asp Tyr Ser Gln 
    610                 615                 620                 


Pro Ser Thr Ser Ser Ser Ile Ile Tyr Ser Ser Gln Glu Asp Val Lys 
625                 630                 635                 640 


Glu Phe Glu Arg Glu Glu Thr Gln Asp Lys Glu Glu Ser Val Glu Ser 
                645                 650                 655     


Ser Leu Pro Leu Asn Ala Ile Glu Pro Cys Val Ile Cys Gln Gly Arg 
            660                 665                 670         


Pro Lys Asn Gly Cys Ile Val His Gly Lys Thr Gly His Leu Met Ala 
        675                 680                 685             


Cys Phe Thr Cys Ala Lys Lys Leu Lys Lys Arg Asn Lys Pro Cys Pro 
    690                 695                 700                 


Val Cys Arg Gln Pro Ile Gln Met Ile Val Leu Thr Tyr Phe Pro 
705                 710                 715                 


<210>  3
<211>  2160
<212>  DNA
<213>  squence artificielle : protine de fusion

<400>  3
atggtttcag ttattaagcc agaaatgaag attaagttgt gtatgagagg tacagttaat     60

ggtcataatt tcgttattga aggtgaaggt aaaggtaatc catacgaagg tactcaaatt    120

ttggatttga atgttactga aggtgctcca ttgccatttg cttacgatat tttgactaca    180

gtttttcaat acggtaatag agcttttact aagtacccag cagatattca agattacttt    240

aagcaaactt ttccagaagg ttaccattgg gaaagatcaa tgacttacga agatcaagga    300

atttgtactg ctacttctaa tatttctatg agaggagatt gttttttcta cgatattaga    360

ttcgatggta ctaattttcc accaaatggt ccagttatgc aaaaaaagac tttgaagtgg    420

gaaccatcta cagaaaagat gtacgttgaa gatggtgttt tgaagggtga tgttaatatg    480

agattgttgt tggaaggtgg tggtcattac agatgtgatt ttaagactac ttacaaggct    540

aaaaaggaag ttagattgcc agatgctcat aagatagatc atagaataga aattttgaag    600

cacgataagg attacaacaa ggttaagtta tacgaaaatg ctgttgcaag atactcaatg    660

ttgccatctc aagctaagga attcatgtgc aataccaaca tgtctgtacc tactgatggt    720

gctgtaacca cctcacagat tccagcttcg gaacaagaga ccctggttag accaaagcca    780

ttgcttttga agttattaaa gtctgttggt gcacaaaaag acacttatac tatgaaagag    840

gttctttttt atcttggcca gtatattatg actaaacgat tatatgatga gaagcaacaa    900

catattgtat attgttcaaa tgatcttcta ggagatttgt ttggcgtgcc aagcttctct    960

gtgaaagagc acaggaaaat atataccatg atctacagga acttggtagt agtcaatcag   1020

caggaatcat cggactcagg tacatctgtg agtgagaaca ggtgtcacct tgaaggtggg   1080

agtgatcaaa aggaccttgt acaagagctt caggaagaga aaccttcatc ttcacatttg   1140

gtttctagac catctacctc atctagaagg agagcaatta gtgagacaga agaaaattca   1200

gatgaattat ctggtgaacg acaaagaaaa cgccacaaat ctgatagtat ttccctttcc   1260

tttgatgaaa gcctggctct gtgtgtaata agggagatat gttgtgaaag aagcagtagc   1320

agtgaatcta cagggacgcc atcgaatccg gatcttgatg ctggtgtaag tgaacattca   1380

ggtgattggt tggatcagga ttcagtttca gatcagttta gtgtagaatt tgaagttgaa   1440

tctctcgact cagaagatta tagccttagt gaagaaggac aagaactctc agatgaagat   1500

gatgaggtat atcaagttac tgtgtatcag gcaggggaga gtgatacaga ttcatttgaa   1560

gaagatcctg aaatttcctt agctgactat tggaaatgca cttcatgcaa tgaaatgaat   1620

cccccccttc catcacattg caacagatgt tgggcccttc gtgagaattg gcttcctgaa   1680

gataaaggga aagataaagg ggaaatctct gagaaagcca aactggaaaa ctcaacacaa   1740

gctgaagagg gctttgatgt tcctgattgt aaaaaaacta tagtgaatga ttccagagag   1800

tcatgtgttg aggaaaatga tgataaaatt acacaagctt cacaatcaca agaaagtgaa   1860

gactattctc agccatcaac ttctagtagc attatttata gcagccaaga agatgtgaaa   1920

gagtttgaaa gggaagaaac ccaagacaaa gaagagagtg tggaatctag tttgcccctt   1980

aatgccattg aaccttgtgt gatttgtcaa ggtcgaccta aaaatggttg cattgtccat   2040

ggcaaaacag gacatcttat ggcctgcttt acatgtgcaa agaagctaaa gaaaaggaat   2100

aagccctgcc cagtatgtag acaaccaatt caaatgattg tgctaactta tttcccctag   2160


<210>  4
<211>  9
<212>  PRT
<213>  squence artificielle : antigne HA

<400>  4

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  5
<211>  8
<212>  PRT
<213>  squence artificielle : anigne FLAG monomre

<400>  5

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  6
<211>  23
<212>  PRT
<213>  squence artificielle : anigne FLAG trimre

<400>  6

Met Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp 
1               5                   10                  15      


Tyr Lys Asp Asp Asp Asp Lys 
            20              


<210>  7
<211>  35
<212>  DNA
<213>  squence artificielle : amorce

<400>  7
gctgggtacc ttaataatca tattacatgg catta                                35


<210>  8
<211>  35
<212>  DNA
<213>  squence artificielle : amorce

<400>  8
cgacctcgag tatagttttt tctccttgac gttaa                                35


<210>  9
<211>  44
<212>  DNA
<213>  squence artificielle : amorce

<400>  9
ggcggcggcc gccaccgcgg tgggcgaatt tcttatgatt tatg                      44


<210>  10
<211>  30
<212>  DNA
<213>  squence artificielle : amorce

<400>  10
ggcggagctc tggaagaacg attacaacag                                      30


<210>  11
<211>  32
<212>  DNA
<213>  squence artificielle : amorce

<400>  11
gcgcgaattc atgtgcaata ccaacatgtc tg                                   32


<210>  12
<211>  37
<212>  DNA
<213>  squence artificielle : amorce

<400>  12
ggcggcggcc gcctagggga aataagttag cacaatc                              37


