                         SEQUENCE LISTING

<110>  Emory University
 
<120>  Immunogenic compositions and methods of use thereof

<130>  050508-2730

<140>  TBD
<141>  2008-12-17

<150>  60/014,130
<151>  2007-12-17

<150>  60/014,130
<151>  2008-07-08

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer

<400>  1
ggttctagaa tgaaattctt agtc                                              24


<210>  2
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer

<400>  2
gtgggatcct ttcatgttga tcgg                                              24


<210>  3
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer

<400>  3
gcaggatcca tggcacaagt cat                                               23


<210>  4
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer

<400>  4
cgcgaattca cgcagtaaag agag                                              24


<210>  5
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer

<400>  5
gctagaattc cagattctgg cgatc                                             25


<210>  6
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer

<400>  6
gctagggccc ttatcagatg catattct                                          28


<210>  7
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer

<400>  7
gctcgtcgac atgaaattct tag                                               23


<210>  8
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer

<400>  8
gctactcgag ttatcagatg catattc                                           27


<210>  9
<211>  1695
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of modified flagellin adjuvunctal polypeptide
       with melltinin signal peptide and influenza hemagglutinin A 
       transmembrane-cytoplasmic tail

<400>  9
atgaaattct tagtcaacgt tgcccttgtt tttatggtcg tgtacatttc ttacatctat       60

gcggacccga tcaacatgac cggatccatg gcacaagtca ttaatacaaa cagcctgtcg      120

ctgttgaccc agaataacct gaacaaatcc cagtccgctc tgggcaccgc tatcgagcgt      180

ctgtcttccg gtctgcgtat caacagcgcg aaagacgatg cggcaggtca ggcgattgct      240

aaccgtttta ccgcgaacat caaaggtctg actcaggctt cccgtaacgc taacgacggt      300

atctccattg cgcagaccac tgaaggcgcg ctgaacgaaa tcaacaacaa cctgcagcgt      360

gtgcgtgaac tggcggttca gtctgctaac agcaccaact cccagtctga cctcgactcc      420

atccaggctg aaatcaccca gcgcctgaac gaaatcgacc gtgtatccgg ccagactcag      480

ttcaacggcg tgaaagtcct ggcgcaggac aacaccctga ccatccaggt tggtgccaac      540

gacggtgaaa ctatcgatat cgatctgaag cagatcaact ctcagaccct gggtctggat      600

acgctgaatg tgcaacaaaa atataaggtc agcgatacgg ctgcaactgt tacaggatat      660

gccgatacaa cgattgcttt agacaatagt acttttaaag cctcggctac tggtcttggt      720

ggtactgacc agaaaattga tggcgattta aaatttgatg atacgactgg aaaatattac      780

gccaaagtta ccgttacggg gggaactggt aaagatggct attatgaagt ttccgttgat      840

aagacgaacg gtgaggagac tcttgctggc ggtgcgactt ccccgcttac aggtggacta      900

cctgcgacag caactgagga tgtgaaaaat gtacaagttg caaatgctga tttgacagag      960

gctaaagccg cattgacagc agcaggtgtt accggcacag catctgttgt taagatgtct     1020

tatactgata ataacggtaa aactattgat ggtggtttag cagttaaggt aggcgatgat     1080

tactattctg caactcaaaa taaagatggt tccataagta ttaatactac gaaatacact     1140

gcagatgacg gtacatccaa aactgcacta aacaaactgg gtgacgcaga cggcaaaacc     1200

gaagttgttt ctattggtgg taaaacttac gctgcaagta aagccgaagg tcacaacttt     1260

aaagcacagc ctgatctggc ggaagcggct gctacaacca ccgaaaaccc gctgcagaaa     1320

attgatgctg ctttggcaca ggttgacacg ttacgttctg acctgggtgc ggtacagaac     1380

cgtttcaact ccgctattac caacctgggc aacaccgtaa acaacctgac ttctgcccgt     1440

agccgtatcg aagattccga ctacgcgacc gaagtttcca acatgtctcg cgcgcagatt     1500

ctgcagcagg ccggtacctc cgttctggcg caggcgaacc aggttccgca aaacgtcctc     1560

tctttactgc gtgaattcca gattctggcg atctactcaa ctgtcgccag ttcactggtg     1620

cttttggtct ccctgggggc aatcagtttc tggatgtgtt ctaatggatc tttgcagtgc     1680

agaatatgca tctga                                                      1695


<210>  10
<211>  564
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of modified flagellin adjuvunctal polypeptide
       with melltinin signal peptide and influenza hemagglutinin A 
       transmembrane-cytoplasmic tail

<400>  10

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala Asp Pro Ile Asn Met Thr Gly Ser Met Ala Gln 
            20                  25                  30          


Val Ile Asn Thr Asn Ser Leu Ser Leu Leu Thr Gln Asn Asn Leu Asn 
        35                  40                  45              


Lys Ser Gln Ser Ala Leu Gly Thr Ala Ile Glu Arg Leu Ser Ser Gly 
    50                  55                  60                  


Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Gln Ala Ile Ala 
65                  70                  75                  80  


Asn Arg Phe Thr Ala Asn Ile Lys Gly Leu Thr Gln Ala Ser Arg Asn 
                85                  90                  95      


Ala Asn Asp Gly Ile Ser Ile Ala Gln Thr Thr Glu Gly Ala Leu Asn 
            100                 105                 110         


Glu Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu Ala Val Gln Ser 
        115                 120                 125             


Ala Asn Ser Thr Asn Ser Gln Ser Asp Leu Asp Ser Ile Gln Ala Glu 
    130                 135                 140                 


Ile Thr Gln Arg Leu Asn Glu Ile Asp Arg Val Ser Gly Gln Thr Gln 
145                 150                 155                 160 


Phe Asn Gly Val Lys Val Leu Ala Gln Asp Asn Thr Leu Thr Ile Gln 
                165                 170                 175     


Val Gly Ala Asn Asp Gly Glu Thr Ile Asp Ile Asp Leu Lys Gln Ile 
            180                 185                 190         


Asn Ser Gln Thr Leu Gly Leu Asp Thr Leu Asn Val Gln Gln Lys Tyr 
        195                 200                 205             


Lys Val Ser Asp Thr Ala Ala Thr Val Thr Gly Tyr Ala Asp Thr Thr 
    210                 215                 220                 


Ile Ala Leu Asp Asn Ser Thr Phe Lys Ala Ser Ala Thr Gly Leu Gly 
225                 230                 235                 240 


Gly Thr Asp Gln Lys Ile Asp Gly Asp Leu Lys Phe Asp Asp Thr Thr 
                245                 250                 255     


Gly Lys Tyr Tyr Ala Lys Val Thr Val Thr Gly Gly Thr Gly Lys Asp 
            260                 265                 270         


Gly Tyr Tyr Glu Val Ser Val Asp Lys Thr Asn Gly Glu Glu Thr Leu 
        275                 280                 285             


Ala Gly Gly Ala Thr Ser Pro Leu Thr Gly Gly Leu Pro Ala Thr Ala 
    290                 295                 300                 


Thr Glu Asp Val Lys Asn Val Gln Val Ala Asn Ala Asp Leu Thr Glu 
305                 310                 315                 320 


Ala Lys Ala Ala Leu Thr Ala Ala Gly Val Thr Gly Thr Ala Ser Val 
                325                 330                 335     


Val Lys Met Ser Tyr Thr Asp Asn Asn Gly Lys Thr Ile Asp Gly Gly 
            340                 345                 350         


Leu Ala Val Lys Val Gly Asp Asp Tyr Tyr Ser Ala Thr Gln Asn Lys 
        355                 360                 365             


Asp Gly Ser Ile Ser Ile Asn Thr Thr Lys Tyr Thr Ala Asp Asp Gly 
    370                 375                 380                 


Thr Ser Lys Thr Ala Leu Asn Lys Leu Gly Asp Ala Asp Gly Lys Thr 
385                 390                 395                 400 


Glu Val Val Ser Ile Gly Gly Lys Thr Tyr Ala Ala Ser Lys Ala Glu 
                405                 410                 415     


Gly His Asn Phe Lys Ala Gln Pro Asp Leu Ala Glu Ala Ala Ala Thr 
            420                 425                 430         


Thr Thr Glu Asn Pro Leu Gln Lys Ile Asp Ala Ala Leu Ala Gln Val 
        435                 440                 445             


Asp Thr Leu Arg Ser Asp Leu Gly Ala Val Gln Asn Arg Phe Asn Ser 
    450                 455                 460                 


Ala Ile Thr Asn Leu Gly Asn Thr Val Asn Asn Leu Thr Ser Ala Arg 
465                 470                 475                 480 


Ser Arg Ile Glu Asp Ser Asp Tyr Ala Thr Glu Val Ser Asn Met Ser 
                485                 490                 495     


Arg Ala Gln Ile Leu Gln Gln Ala Gly Thr Ser Val Leu Ala Gln Ala 
            500                 505                 510         


Asn Gln Val Pro Gln Asn Val Leu Ser Leu Leu Arg Glu Phe Gln Ile 
        515                 520                 525             


Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu Val Ser 
    530                 535                 540                 


Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys 
545                 550                 555                 560 


Arg Ile Cys Ile 
                


<210>  11
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  MHC-1 HA peptide 1

<400>  11

Ile Tyr Ser Thr Val Ala Ser Ser Leu 
1               5                   


<210>  12
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  MHC-1 HA peptide 2

<400>  12

Leu Tyr Glu Lys Val Lys Ser Gln Leu 
1               5                   


<210>  13
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  MHC-II HA peptide 1

<400>  13

Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Glu 
1               5                   10      


<210>  14
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  MHC-II HA peptide 2

<400>  14

His Asn Thr Asn Gly Val Thr Ala Ala Cys Ser His 
1               5                   10          


<210>  15
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  MHC-II HA peptide 3

<400>  15

Cys Pro Lys Tyr Val Arg Ser Ala Lys Leu Arg Met 
1               5                   10          


<210>  16
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  MHC-II HA peptide 4

<400>  16

Lys Leu Lys Asn Ser Tyr Val Asn Lys Lys Gly Lys 
1               5                   10          


<210>  17
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  MHC-II HA peptide 5

<400>  17

Asn Ala Tyr Val Ser Val Val Thr Ser Lys Tyr Asn Arg Arg Phe 
1               5                   10                  15  


<210>  18
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Flexible linker sequence

<400>  18

Gly Ala Pro Val Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Trp 
1               5                   10                  15      


