                         SEQUENCE LISTING

<110>  University of Pennsylvania
       Smith, Douglas
       Stewart, William
       Johnson, Victoria
 
<120>  Neprilysin Gene Polymorphism and Amyloid b Plaques in Traumatic 
       Brain Injury

<130>  046483-5130-00-WO 431489

<150>  61/007,729
<151>  2007-12-14

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  5643
<212>  DNA
<213>  Homo sapiens

<400>  1
gagagcgccg agacgcgcgg ggcgcggaga tgtgcaagtg gcgaagcttg accgagagca       60

ggctggagca gccgcccaac tcctggcgcg ggatctgctg aggggtcacg attttaggtg      120

atgggcaagt cagaaagtca gatggatata actgatatca acactccaaa gccaaagaag      180

aaacagcgat ggactccact ggagatcagc ctctcggtcc ttgtcctgct cctcaccatc      240

atagctgtga caatgatcgc actctatgca acctacgatg atggtatttg caagtcatca      300

gactgcataa aatcagctgc tcgactgatc caaaacatgg atgccaccac tgagccttgt      360

acagactttt tcaaatatgc ttgcggaggc tggttgaaac gtaatgtcat tcccgagacc      420

agctcccgtt acggcaactt tgacatttta agagatgaac tagaagtcgt tttgaaagat      480

gtccttcaag aacccaaaac tgaagatata gtagcagtgc agaaagcaaa agcattgtac      540

aggtcttgta taaatgaatc tgctattgat agcagaggtg gagaacctct actcaaactg      600

ttaccagaca tatatgggtg gccagtagca acagaaaact gggagcaaaa atatggtgct      660

tcttggacag ctgaaaaagc tattgcacaa ctgaattcta aatatgggaa aaaagtcctt      720

attaatttgt ttgttggcac tgatgataag aattctgtga atcatgtaat tcatattgac      780

caacctcgac ttggcctccc ttctagagat tactatgaat gcactggaat ctataaagag      840

gcttgtacag catatgtgga ttttatgatt tctgtggcca gattgattcg tcaggaagaa      900

agattgccca tcgatgaaaa ccagcttgct ttggaaatga ataaagttat ggaattggaa      960

aaagaaattg ccaatgctac ggctaaacct gaagatcgaa atgatccaat gcttctgtat     1020

aacaagatga cattggccca gatccaaaat aacttttcac tagagatcaa tgggaagcca     1080

ttcagctggt tgaatttcac aaatgaaatc atgtcaactg tgaatattag tattacaaat     1140

gaggaagatg tggttgttta tgctccagaa tatttaacca aacttaagcc cattcttacc     1200

aaatattctg ccagagatct tcaaaattta atgtcctgga gattcataat ggatcttgta     1260

agcagcctca gccgaaccta caaggagtcc agaaatgctt tccgcaaggc cctttatggt     1320

acaacctcag aaacagcaac ttggagacgt tgtgcaaact atgtcaatgg gaatatggaa     1380

aatgctgtgg ggaggcttta tgtggaagca gcatttgctg gagagagtaa acatgtggtc     1440

gaggatttga ttgcacagat ccgagaagtt tttattcaga ctttagatga cctcacttgg     1500

atggatgccg agacaaaaaa gagagctgaa gaaaaggcct tagcaattaa agaaaggatc     1560

ggctatcctg atgacattgt ttcaaatgat aacaaactga ataatgagta cctcgagttg     1620

aactacaaag aagatgaata cttcgagaac ataattcaaa atttgaaatt cagccaaagt     1680

aaacaactga agaagctccg agaaaaggtg gacaaagatg agtggataag tggagcagct     1740

gtagtcaatg cattttactc ttcaggaaga aatcagatag tcttcccagc cggcattctg     1800

cagcccccct tctttagtgc ccagcagtcc aactcattga actatggggg catcggcatg     1860

gtcataggac acgaaatcac ccatggcttc gatgacaatg gcagaaactt taacaaagat     1920

ggagacctcg ttgactggtg gactcaacag tctgcaagta actttaagga gcaatcccag     1980

tgcatggtgt atcagtatgg aaacttttcc tgggacctgg caggtggaca gcaccttaat     2040

ggaattaata cactgggaga aaacattgct gataatggag gtcttggtca agcatacaga     2100

gcctatcaga attatattaa aaagaatggc gaagaaaaat tacttcctgg acttgaccta     2160

aatcacaaac aactattttt cttgaacttt gcacaggtgt ggtgtggaac ctataggcca     2220

gagtatgcgg ttaactccat taaaacagat gtgcacagtc caggcaattt caggattatt     2280

gggactttgc agaactctgc agagttttca gaagcctttc actgccgcaa gaattcatac     2340

atgaatccag aaaagaagtg ccgggtttgg tgatcttcaa aagaagcatt gcagcccttg     2400

gctagacttg ccaacaccac agaaatgggg aattctctaa tcgaaagaaa atgggcccta     2460

ggggtcactg tactgacttg agggtgatta acagagaggg caccatcaca atacagataa     2520

cattaggttg tcctagaaag ggtgtggagg gaggaagggg gtctaaggtc tatcaagtca     2580

atcatttctc actgtgtaca taatgcttaa tttctaaaga taatattact gtttatttct     2640

gtttctcata tggtctacca gtttgctgat gtccctagaa aacaatgcaa aacctttgag     2700

gtagaccagg atttctaatc aaaagggaaa agaagatgtt gaagaataca gttaggcacc     2760

agaagaacag taggtgacac tatagtttaa aacacattgc ctaactacta gtttttactt     2820

ttatttgcaa catttacagt ccttcaaaat ccttccaaag aattcttata cacattgggg     2880

ccttggagct tacatagttt taaactcatt tttgccatac atcagttatt cattctgtga     2940

tcatttattt taagcactct taaagcaaaa aatgaatgtc taaaattgtt ttttgttgta     3000

cctgctttga ctgatgctga gattcttcag gcttcctgca attttctaag caatttcttg     3060

ctctatctct caaaacttgg tatttttcag agatttatat aaatgtaaaa ataataattt     3120

ttatatttaa ttattaacta catttatgag taactattat tataggtaat caatgaatat     3180

tgaagtttca gcttaaaata aacagttgtg aaccaagatc tataaagcga tatacagatg     3240

aaaatttgag actatttaaa cttataaatc atattgatga aaagatttaa gcacaaactt     3300

tagggtaaaa attgccattg gacagttgtc tagagatata tatacttgtg gttttcaaat     3360

tggactttca aaattaaatc tgtccctgag agtgtctctg ataaaagggc aaatctgcac     3420

ctatgtagct ctgcatctcc tgtcttttca ggtttgtcat cagatggaaa tattttgata     3480

ataaattgaa attgtgaact cattgctccc taagactgtg acaactgtct aactttagaa     3540

gtgcatttct gaatagaaat gggaggcctc tgatggacct tctagaatta taagtcacaa     3600

agagttctgg aaaagaactg tttactgctt gataggaatt catcttttga ggcttctgtt     3660

cctctctttt cctgttgtat tgactatttt cgttcattac ttgattaaga ttttacaaaa     3720

gaggagcact tccaaaattc ttatttttcc taacaaaaga tgaaagcagg gaatttctat     3780

ctaaatgatg agtattagtt ccctgtctct tgaaaaatgc ccatttgcct ttaaaaaaaa     3840

aagttacaga aatactataa catatgtaca taaattgcat aaagcataag tatacagttc     3900

aataaactta actttaactg aacaatggcc ctgtagccag cacctgtaag aaacagagca     3960

gtaccagcgc tctaaaagca cctccttgtc actttattac tcccagaaca acaactatcc     4020

tgacttctaa tatcattcac tagctttgcc tggttttgtc ttttatgcag atagaatcaa     4080

tcagtatgta ttcttttgtg cctggcttct ttctctcagc cttacatttg tgagattcct     4140

ctgtattgtg ctgattgtgg atcttttcat tctcattgca gaataatgtt ctattgtggg     4200

acttattaca atttgttcat cctattgttg atgggcactt gagaactttc cattttggcg     4260

ctattacaaa tagtgcaact atgaatgtac tgcatgttac catcttactt gagcctttaa     4320

tggacttatt tcttcaaatc cttccaaaaa ttattataag cattgaaatt atagtttcaa     4380

gccaactgtg gataccctta ccctttcctc ctttatcaca accaccgtta caagtatact     4440

tatatttccc taaaatacat ttaaaactta cctaagtgac atttgtagtt ggagtaatag     4500

gagcttccag ctctaataaa acagctgtct ctaacttatt ttatttccat catgtcagag     4560

caggtgaaga gccagaagtg aagagtgact agtacaaatt ataaaaagcc actagactct     4620

tcactgttag ctttttaaaa cattaggctc ccatccctat ggaggaacaa ctctccagtg     4680

cctggatccc ctctgtctac aaatataaga ttttctgggc ctaaaggata gatcaaagtc     4740

aaaaatagca atgcctccct atccctcaca catccagaca tcatgaattt tacatggtac     4800

tcttgttgag ttctgtagag ccttctgatg tctctaaagc actaccgatt ctttggagtt     4860

gtcacatcag ataagacata tctctaattc catccataaa tccagttcta ctatggctga     4920

gttctggtca aagaaagaaa gtttagaagc tgagacacaa agggttggga gctgatgaaa     4980

ctcacaaatg atggtaggaa gaagctctcg acaatacccg ttggcaagga gtctgcctcc     5040

atgctgcagt gttcgagtgg attgtaggtg caagatggaa aggattgtag gtgcaagctg     5100

tccagagaaa agagtccttg ttccagccct attctgccac tcctgacagg gtgaccttgg     5160

gtatttgcaa tattcctttg ggcctctgct tctctcacct aaaaaaagag aattagatta     5220

tattggtggt tctcagcaag agaaggagta tgtgtccaat gctgccttcc catgaatctg     5280

tctcccagtt atgaatcagt gggcaggata aactgaaaac tcccatttac gtgtctgaat     5340

cgagtgagac aaaattttag tccaaataac aagtaccaaa gttttatcaa gtttgggtct     5400

gtgctgctgt tactgttaac catttaagtg gggcaaaacc ttgctaattt tctcaaaagc     5460

atttatcatt cttgttgcca cagctggagc tctcaaacta aaagacattt gttattttgg     5520

aaagaagaaa gactctattc tcaaagtttc ctaatcagaa atttttatca gtttccagtc     5580

tcaaaaatac aaaataaaaa caaacgtttt taatactatt gcttttatgc ctagtcaact     5640

ctg                                                                   5643


<210>  2
<211>  750
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Gly Lys Ser Glu Ser Gln Met Asp Ile Thr Asp Ile Asn Thr Pro 
1               5                   10                  15      


Lys Pro Lys Lys Lys Gln Arg Trp Thr Pro Leu Glu Ile Ser Leu Ser 
            20                  25                  30          


Val Leu Val Leu Leu Leu Thr Ile Ile Ala Val Thr Met Ile Ala Leu 
        35                  40                  45              


Tyr Ala Thr Tyr Asp Asp Gly Ile Cys Lys Ser Ser Asp Cys Ile Lys 
    50                  55                  60                  


Ser Ala Ala Arg Leu Ile Gln Asn Met Asp Ala Thr Thr Glu Pro Cys 
65                  70                  75                  80  


Thr Asp Phe Phe Lys Tyr Ala Cys Gly Gly Trp Leu Lys Arg Asn Val 
                85                  90                  95      


Ile Pro Glu Thr Ser Ser Arg Tyr Gly Asn Phe Asp Ile Leu Arg Asp 
            100                 105                 110         


Glu Leu Glu Val Val Leu Lys Asp Val Leu Gln Glu Pro Lys Thr Glu 
        115                 120                 125             


Asp Ile Val Ala Val Gln Lys Ala Lys Ala Leu Tyr Arg Ser Cys Ile 
    130                 135                 140                 


Asn Glu Ser Ala Ile Asp Ser Arg Gly Gly Glu Pro Leu Leu Lys Leu 
145                 150                 155                 160 


Leu Pro Asp Ile Tyr Gly Trp Pro Val Ala Thr Glu Asn Trp Glu Gln 
                165                 170                 175     


Lys Tyr Gly Ala Ser Trp Thr Ala Glu Lys Ala Ile Ala Gln Leu Asn 
            180                 185                 190         


Ser Lys Tyr Gly Lys Lys Val Leu Ile Asn Leu Phe Val Gly Thr Asp 
        195                 200                 205             


Asp Lys Asn Ser Val Asn His Val Ile His Ile Asp Gln Pro Arg Leu 
    210                 215                 220                 


Gly Leu Pro Ser Arg Asp Tyr Tyr Glu Cys Thr Gly Ile Tyr Lys Glu 
225                 230                 235                 240 


Ala Cys Thr Ala Tyr Val Asp Phe Met Ile Ser Val Ala Arg Leu Ile 
                245                 250                 255     


Arg Gln Glu Glu Arg Leu Pro Ile Asp Glu Asn Gln Leu Ala Leu Glu 
            260                 265                 270         


Met Asn Lys Val Met Glu Leu Glu Lys Glu Ile Ala Asn Ala Thr Ala 
        275                 280                 285             


Lys Pro Glu Asp Arg Asn Asp Pro Met Leu Leu Tyr Asn Lys Met Thr 
    290                 295                 300                 


Leu Ala Gln Ile Gln Asn Asn Phe Ser Leu Glu Ile Asn Gly Lys Pro 
305                 310                 315                 320 


Phe Ser Trp Leu Asn Phe Thr Asn Glu Ile Met Ser Thr Val Asn Ile 
                325                 330                 335     


Ser Ile Thr Asn Glu Glu Asp Val Val Val Tyr Ala Pro Glu Tyr Leu 
            340                 345                 350         


Thr Lys Leu Lys Pro Ile Leu Thr Lys Tyr Ser Ala Arg Asp Leu Gln 
        355                 360                 365             


Asn Leu Met Ser Trp Arg Phe Ile Met Asp Leu Val Ser Ser Leu Ser 
    370                 375                 380                 


Arg Thr Tyr Lys Glu Ser Arg Asn Ala Phe Arg Lys Ala Leu Tyr Gly 
385                 390                 395                 400 


Thr Thr Ser Glu Thr Ala Thr Trp Arg Arg Cys Ala Asn Tyr Val Asn 
                405                 410                 415     


Gly Asn Met Glu Asn Ala Val Gly Arg Leu Tyr Val Glu Ala Ala Phe 
            420                 425                 430         


Ala Gly Glu Ser Lys His Val Val Glu Asp Leu Ile Ala Gln Ile Arg 
        435                 440                 445             


Glu Val Phe Ile Gln Thr Leu Asp Asp Leu Thr Trp Met Asp Ala Glu 
    450                 455                 460                 


Thr Lys Lys Arg Ala Glu Glu Lys Ala Leu Ala Ile Lys Glu Arg Ile 
465                 470                 475                 480 


Gly Tyr Pro Asp Asp Ile Val Ser Asn Asp Asn Lys Leu Asn Asn Glu 
                485                 490                 495     


Tyr Leu Glu Leu Asn Tyr Lys Glu Asp Glu Tyr Phe Glu Asn Ile Ile 
            500                 505                 510         


Gln Asn Leu Lys Phe Ser Gln Ser Lys Gln Leu Lys Lys Leu Arg Glu 
        515                 520                 525             


Lys Val Asp Lys Asp Glu Trp Ile Ser Gly Ala Ala Val Val Asn Ala 
    530                 535                 540                 


Phe Tyr Ser Ser Gly Arg Asn Gln Ile Val Phe Pro Ala Gly Ile Leu 
545                 550                 555                 560 


Gln Pro Pro Phe Phe Ser Ala Gln Gln Ser Asn Ser Leu Asn Tyr Gly 
                565                 570                 575     


Gly Ile Gly Met Val Ile Gly His Glu Ile Thr His Gly Phe Asp Asp 
            580                 585                 590         


Asn Gly Arg Asn Phe Asn Lys Asp Gly Asp Leu Val Asp Trp Trp Thr 
        595                 600                 605             


Gln Gln Ser Ala Ser Asn Phe Lys Glu Gln Ser Gln Cys Met Val Tyr 
    610                 615                 620                 


Gln Tyr Gly Asn Phe Ser Trp Asp Leu Ala Gly Gly Gln His Leu Asn 
625                 630                 635                 640 


Gly Ile Asn Thr Leu Gly Glu Asn Ile Ala Asp Asn Gly Gly Leu Gly 
                645                 650                 655     


Gln Ala Tyr Arg Ala Tyr Gln Asn Tyr Ile Lys Lys Asn Gly Glu Glu 
            660                 665                 670         


Lys Leu Leu Pro Gly Leu Asp Leu Asn His Lys Gln Leu Phe Phe Leu 
        675                 680                 685             


Asn Phe Ala Gln Val Trp Cys Gly Thr Tyr Arg Pro Glu Tyr Ala Val 
    690                 695                 700                 


Asn Ser Ile Lys Thr Asp Val His Ser Pro Gly Asn Phe Arg Ile Ile 
705                 710                 715                 720 


Gly Thr Leu Gln Asn Ser Ala Glu Phe Ser Glu Ala Phe His Cys Arg 
                725                 730                 735     


Lys Asn Ser Tyr Met Asn Pro Glu Lys Lys Cys Arg Val Trp 
            740                 745                 750 


<210>  3
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Chemically synthesized

<400>  3
tttcagtatg aattccgcag t                                                 21


<210>  4
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  Chemically synthesized

<400>  4
tgatcccttt cctcttttga at                                                22


