                         SEQUENCE LISTING

<110>  Carnegie Mellon University
 
<120>  LINKED PEPTIDE FLUOROGENIC BIOSENSORS

<130>  080750PCT

<150>  61/005,122
<151>  2007-12-03

<160>  58    

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PNNL yeast scFv library linker sequence

<400>  1

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  2
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker DNA Sequence

<400>  2
ggtggcggtg gcagcggcgg tggtggttcc ggaggcggcg gttct                       45


<210>  3
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin Short Recognition Sequence

<400>  3

Arg Lys Lys Arg Ser 
1               5   


<210>  4
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Furin Short Recognition Sequence DNA

<400>  4
agaaagaaga gatct                                                        15


<210>  5
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin Long Recognition Sequence

<400>  5

Asn Ser Arg Lys Lys Arg Ser Thr Ser Ala 
1               5                   10  


<210>  6
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Furin Long Recognition Sequence DNA

<400>  6
aactcgagaa agaagagatc tacctccgct                                        30


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TEV Protease Recognition Sequence

<400>  7

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEV Protease Recognition Sequence DNA

<400>  8
gaaaacctat acttccaagg t                                                 21


<210>  9
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRV-3C Protease Recognition Sequence

<400>  9

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


<210>  10
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HRV-3C Protease Recognition Sequence DNA

<400>  10
ttggaagttt tgttccaagg tcca                                              24


<210>  11
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Caspase 1 Protease Recognition Sequence

<400>  11

Tyr Val Ala Asp 
1               


<210>  12
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Caspase 1 Protease Recognition Sequence DNA

<400>  12
tacgttgctg ac                                                           12


<210>  13
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Caspase 3 Protease Recognition Sequence

<400>  13

Asp Glu Val Asp 
1               


<210>  14
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Caspase 3 Protease Recognition Sequence DNA

<400>  14
gacgaagttg ac                                                           12


<210>  15
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MMP25 Recognition Sequence

<400>  15

Val Met Arg Leu Val Val 
1               5       


<210>  16
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MMP25 Recognition Sequence DNA

<400>  16
gtcatgagat tggtcgtc                                                     18


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PKA Kemptide Recognition Sequence

<400>  17

Leu Leu Arg Arg Ala Ser Leu Gly Pro 
1               5                   


<210>  18
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PKA Kemptide DNA

<400>  18
ttgttgagaa gagctagttt gggtcca                                           27


<210>  19
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H3-K56 histone acetyltransferase recognition sequence

<400>  19

Ile Arg Arg Phe Gln Lys Ser Thr Asp Leu Leu 
1               5                   10      


<210>  20
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H3-K56 HAT Recognition Sequence DNA

<400>  20
atcagaagat tccaaaagtc caccgacttg ttg                                    33


<210>  21
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  21

Gln Val Gln Leu Val Glu Ser Glu Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Leu Leu Asp Thr Thr Met Val Thr Gly Tyr Tyr Phe Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Ile Leu Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asn Phe 
    130                 135                 140                 


Met Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln Ser Val 
145                 150                 155                 160 


Thr Ile Ser Cys Ser Gly Ser Gly Ser Asn Ile Gly Asn Asn Lys Val 
                165                 170                 175     


Asn Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile Tyr 
            180                 185                 190         


Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln Ser Glu 
    210                 215                 220                 


Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser Leu Asn Gly 
225                 230                 235                 240 


Tyr Val Phe Gly Thr Gly Thr Lys Leu Thr Val Leu 
                245                 250         


<210>  22
<211>  1221
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  22
cattttcaat taagatgcag ttacttcgct gtttttcaat attttctgtt attgcttcag       60

ttttagcaca ggaactgaca actatatgcg agcaaatccc ctcaccaact ttagaatcga      120

cgccgtactc tttgtcaacg actactattt tggccaacgg gaaggcaatg caaggagttt      180

ttgaatatta caaatcagta acgtttgtca gtaattgcgg ttctcacccc tcaacaacta      240

gcaaaggcag ccccataaac acacagtatg tttttaagga caatagctcg acgattgaag      300

gtagataccc atacgacgtt ccagactacg ctctgcaggc tagtggtggt ggtggttctg      360

gtggtggtgg ttctggtggt ggtggttctg ctagccaggt gcagctggtg gaatctgagg      420

ctgaggtgaa gaagcctggg tcctcggtga aggtctcctg caaggcttct ggaggcacct      480

tcagcagcta tgctatcagc tgggtgcgac aggcccctgg acaagggctt gagtggatgg      540

gagggatcat ccctatcttt ggtacagcaa actacgcaca gaagttccag ggcagagtca      600

cgattaccgc ggacgaatcc acgagcacag cctacatgga gctgagcagc ctgagatctg      660

aggacacggc cgtgtattac tgtgtcttgt tggatacaac tatggttacg ggatactact      720

ttgactactg gggccaggga accctggtca ccgtctcctc aggaattcta ggatccggtg      780

gcggtggcag cggcggtggt ggttccggag gcggcggttc taattttatg ctgactcagc      840

ccccctcagc gtctgggacc cccgggcaga gcgtcaccat ctcttgttct ggaagcggct      900

cgaacatcgg aaacaataaa gtaaactggt accagcagct cccaggaacg gcccccaaac      960

tcctcatcta tagtaataat cagcggccct caggggtccc tgaccgattc tctggctcca     1020

agtctggcac ctcagcctcc ctggccatca gtgggctcca gtctgaggat gaggctgatt     1080

attactgtgc agcatgggat gacagcctga atggttatgt cttcggaact gggaccaagc     1140

tcaccgtcct atccggaatt ctagaacaaa agcttatttc tgaagaagac ttgtaatagc     1200

tcggcggccg catcgagatc t                                               1221


<210>  23
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  23

Gln Val Gln Leu Val Glu Ser Glu Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Glu Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Arg Ile Asp Gly Asp Gly Ser Ser Thr Asn Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ala Arg Tyr Phe Gly Ser Val Ser Pro Tyr Gly Met Asp Val 
            100                 105                 110         


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Ile Leu Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
    130                 135                 140                 


Ile Arg Val Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly Asp 
145                 150                 155                 160 


Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Gly Ile Ala Thr Trp Leu 
                165                 170                 175     


Gly Trp Tyr Gln Gln Lys Pro Gly Lys Pro Pro Gln Leu Leu Ile Tyr 
            180                 185                 190         


Ser Ala Ser Thr Leu Gln Thr Gly Val Pro Ser Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu 
    210                 215                 220                 


Asp Val Ala Thr Tyr Tyr Cys Gln Glu Gly Ser Thr Phe Pro Leu Thr 
225                 230                 235                 240 


Phe Gly Gly Gly Thr Lys Val Asp Ile Lys Ser Gly Ile Leu Glu Gln 
                245                 250                 255     


Lys Leu Ile Ser Glu Glu Asp Leu 
            260                 


<210>  24
<211>  881
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  24
ctctgcaggc tagtggtggt ggtggttctg gtggtggtgg ttctgctagc caggtgcagc       60

tggtggagtc tgagggaggc ttggtacagc ctggagggtc cctgagactc tcctgtgcag      120

cctctggatt caccttcagt agttatgaaa tgaactgggt ccgccaggct ccaggtaagg      180

ggctggagtg ggtctcacgt attgatggtg atgggagcag cacaaactac gcggactccg      240

tgaagggccg attcaccatc tccagagaca acgccaagag cacgctgtat ctgcaaatga      300

atagtctgag agccgaggac acggctgtgt attactgtac aagggccaga tactttggtt      360

cggtgagccc ctacggtatg gacgtctggg gccaagggac cacggtcacc gtctcctcag      420

gaattctagg atccggtggc ggtggcagcg gcggtggtgg ttccggaggc ggcggttctg      480

acatccgggt gacccagtct ccttcttccg tgtctgcatc tgtgggtgac agagtcacca      540

tcagttgtcg ggcgagtcag gggattgcca cctggttagg ctggtatcag cagaagccag      600

ggaaaccccc tcagctcctt atctattctg catccacttt gcaaactggg gtcccatcaa      660

ggttcagcgg cagtggatct gggacagatt tcactcttac catcagcagc ctgcagccgg      720

aggatgttgc aacttactat tgtcaagagg gtagcacttt ccctctcact ttcggcggag      780

ggaccaaagt ggatatcaaa tccggaattc tagaacaaaa gcttatttct gaagaagact      840

tgtaatagct cggcggccgc atcgagatct gataacaaca g                          881


<210>  25
<211>  268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  25

Gln Val Gln Leu Gln Gln Trp Asp Ala Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Asp Ser Val Ser Ser Asn 
            20                  25                  30          


Ser Ala Ala Trp Asn Trp Ile Arg Gln Ser Pro Ser Arg Gly Leu Glu 
        35                  40                  45              


Trp Pro Gly Arg Thr Tyr Tyr Arg Ser Lys Trp Gln Asn Asn Tyr Ala 
    50                  55                  60                  


Leu Ser Val Gln Gly Arg Ile Thr Ile Asn Pro Asp Thr Ser Asn Asn 
65                  70                  75                  80  


Gln Phe Ser Leu Gln Leu Asp Ser Met Thr Pro Glu Asp Thr Gly Val 
                85                  90                  95      


Tyr Tyr Cys Thr Arg Gly Gly Gly Ser Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Gly Ser Ala Ser Ala Pro Thr Gly Ile 
        115                 120                 125             


Leu Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Ser Tyr Glu Leu Thr Gln Pro Pro Ser Val Ser Val Ser Pro 
145                 150                 155                 160 


Gly Gln Thr Ala Thr Ile Thr Cys Ser Gly Asp Glu Met Gly Asp Lys 
                165                 170                 175     


Tyr Ala Tyr Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val 
            180                 185                 190         


Ile Tyr Lys Asp Ser Glu Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser 
        195                 200                 205             


Gly Ser Ser Ser Gly Thr Thr Val Thr Leu Thr Ile Ser Gly Val Gln 
    210                 215                 220                 


Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Gln Ser Ala Asp Ser Ser Gly 
225                 230                 235                 240 


Thr Ser Val Val Phe Gly Gly Gly Thr Lys Val Thr Val Leu Ser Gly 
                245                 250                 255     


Ile Leu Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
            260                 265             


<210>  26
<211>  908
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  26
ctctgcaggc tagtggtggt ggtggttctg gtggtggtgg ttctggtggt ggtggttctg       60

ctagccaggt gcagctacag cagtgggacg caggactggt gaagccctcg cagaccctct      120

cactcacctg tgccatctcc ggggacagtg tctctagcaa cagtgctgct tggaactgga      180

tcaggcagtc cccatcgaga ggtcttgagt ggccgggaag gacatactac aggtccaagt      240

ggcaaaacaa ttatgcactc tctgtgcaag gtcgaataac catcaaccca gacacatcca      300

acaaccaatt ctccctgcag ctggactcta tgactcccga ggacacgggt gtatattact      360

gtacaagggg cggcgggtcc ttagactact ggggccaggg aaccctggtc accgtctcct      420

cagggagtgc atccgcccca accggaattc taggatccgg tggcggtggc agcggcggtg      480

gtggttccgg aggcggcggt tcttcctatg agctgacaca gccaccctca gtgtccgtgt      540

ccccaggaca gacagccacc atcacctgct ctggagatga aatgggggat aaatatgctt      600

attggtacca gcagaagcca ggccaggccc ctgtgctggt gatatataaa gacagtgaga      660

ggccctcagg gatccctgag cgattctctg gctccagctc agggacaaca gtcaccttga      720

ccatcagtgg agtccaggca gaagacgagg ctgactatta ctgtcaatca gcagacagca      780

gtggtacttc tgtggtattc ggcggaggga ccaaggtcac cgtcctatcc ggaattctag      840

aacaaaagct tatttctgaa gaagacttgt aatagctcgg cggccgcatc gagatctgat      900

aacaacag                                                               908


<210>  27
<211>  279
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  27

Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Arg Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Asp Ile Ser Gly Asp Ser Val Ser Ser Asn 
            20                  25                  30          


Ser Val Ala Trp Asn Trp Ile Arg Gln Ser Pro Ser Arg Gly Leu Glu 
        35                  40                  45              


Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys Trp Ile Asn Glu Tyr Gly 
    50                  55                  60                  


Pro Phe Val Arg Ser Arg Ile Thr Ile Asn Pro Asp Thr Ser Lys Asn 
65                  70                  75                  80  


Gln Phe Ser Leu Gln Leu Asn Ser Val Thr Pro Glu Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Thr Met Ala Asn Ser Gly Tyr Asp Arg Ser Ser Gly 
            100                 105                 110         


His Asn Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val 
        115                 120                 125             


Ser Ser Gly Ser Ala Ser Ala Pro Thr Gly Ile Leu Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Tyr Glu 
145                 150                 155                 160 


Leu Thr Gln Pro Pro Ser Val Ser Val Ser Pro Gly Gln Thr Ala Arg 
                165                 170                 175     


Ile Thr Cys Ser Gly Asp Ala Leu Pro Lys Gln Tyr Thr Tyr Trp Tyr 
            180                 185                 190         


Gln Gln Lys Ala Gly Gln Ala Pro Val Leu Val Ile Tyr Lys Asp Thr 
        195                 200                 205             


Glu Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Thr Ser Ser Gly 
    210                 215                 220                 


Thr Thr Val Thr Leu Thr Ile Ser Gly Val Gln Ala Glu Asp Glu Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Gln Ser Ala Asp Ser Ser Gly Ser Tyr Val Phe Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Val Thr Val Leu Ser Gly Ile Leu Glu Gln Lys 
            260                 265                 270         


Leu Ile Ser Glu Glu Asp Leu 
        275                 


<210>  28
<211>  935
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  28
cgctctgcag gctagtggtg gtggtggttc tggtggtggt ggttctggtg gtggtggttc       60

tgctagccag gtacagctgc agcagtcagg tccaggacgg gtgaagccct cgcagaccct      120

ctcactcacc tgtgacatct ccggggacag tgtctctagc aacagtgttg cttggaactg      180

gatcaggcag tccccatcga gaggccttga gtggctggga aggacatact acaggtccaa      240

gtggattaat gaatatggac catttgtaag aagtcgaata accatcaacc cagacacatc      300

caagaatcag ttctccctgc agttgaactc tgtgactccc gaggacacgg ctgtctatta      360

ctgtgcaaca atggcgaata gtggctacga tcggtcctct ggtcacaact acggaatgga      420

cgtctggggc caagggacca cggtcaccgt ctcctcaggg agtgcatccg ccccaaccgg      480

aattctagga tccggtggcg gtggcagcgg cggtggtggt tccggaggcg gcggttcttc      540

ctatgagttg actcagccac cctcggtgtc agtgtcccca ggacagacgg ccaggatcac      600

ctgctctgga gatgcattgc caaagcaata tacttattgg taccagcaga aggcaggcca      660

ggcccctgtc ttggtgatat ataaagacac tgagaggccc tcagggatcc ctgagcgatt      720

ctctggtacc agttcaggga caacagtcac attgaccatc agtggagtcc aggcagaaga      780

cgaggctgac tattactgtc aatcagcaga cagcagtggt tcctatgttt tcttcggcgg      840

agggaccaag gtgaccgtcc tatccggaat tctagaacaa aagcttattt ctgaagaaga      900

cttgtaatag ctcggcggcc gcatcgagat ctgat                                 935


<210>  29
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  29

Gln Ala Val Val Thr Gln Glu Pro Ser Val Thr Val Ser Pro Gly Gly 
1               5                   10                  15      


Thr Val Ile Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ser Gly 
            20                  25                  30          


His Tyr Ala Asn Trp Phe Gln Gln Lys Pro Gly Gln Ala Pro Arg Ala 
        35                  40                  45              


Leu Ile Phe Glu Thr Asp Lys Lys Tyr Ser Trp Thr Pro Gly Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Leu Gly Ala Lys Ala Ala Leu Thr Ile Ser Asp Ala 
65                  70                  75                  80  


Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Leu Leu Ser Asp Val Asp 
                85                  90                  95      


Gly Tyr Leu Phe Gly Gly Gly Thr Gln Leu Thr Val Leu Ser Gly Ile 
            100                 105                 110         


Leu Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
        115                 120             


<210>  30
<211>  651
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  30
ggctagtggt ggtggtggtt ctggtggtgg tggttctgct agcactggca gctttgactc       60

ctggggccag ggaaccctgg tcaccgtctc ctcaggaatt ctaggatccg gtggcggtgg      120

cagcggcggt ggtggttccg gaggcggcgg ttctcaggct gtggtgactc aggagccgtc      180

agtgactgtg tccccaggag ggacagtcat tctcacttgt ggctccagca ctggagctgt      240

caccagtggt cattatgcca actggttcca gcagaagcct ggccaagccc ccagggcact      300

tatatttgaa accgacaaga aatattcctg gacccctggc cgattctcag gctccctcct      360

tggggccaag gctgccctga ccatctcgga tgcgcagcct gaagatgagg ctgagtatta      420

ctgtttgctc tccgacgttg acggttatct gttcggagga ggcacccagc tgaccgtcct      480

ctccggaatt ctagaacaaa agcttatttc tgaagaagac ttgtaatagc tcggcggccg      540

catcgagatc tgataacaac agtgtagatg taacaaaatc gactttgttc ccactgtact      600

tttagctcgt acaaaataca atatactttt catttctccg taaacaacat g               651


<210>  31
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  31

Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Arg Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Asp Ser Val Pro Lys Asn 
            20                  25                  30          


Gly Ala Ser Trp Asn Trp Ile Arg Leu Ser Pro Ser Arg Gly Leu Glu 
        35                  40                  45              


Trp Leu Gly Arg Thr His Tyr Ser Ser Arg Trp Tyr His Asp Tyr Ala 
    50                  55                  60                  


Phe Phe Val Lys Ser Arg Ile Thr Ile Asn Val Asp Thr Ser Glu Thr 
65                  70                  75                  80  


Gln Val Ser Leu Gln Leu Asp Ser Val Thr Pro Asp Asp Thr Gly Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Ser Gln Arg Arg Gly Trp Phe Asp Leu Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Gln Glu Phe 
        115                 120                 


<210>  32
<211>  853
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  32
ggtggtggtg gttctggtgg tggtggttct gctagccagg tacagctgca gcagtcaggt       60

ccaggactgg tgaggccctc gcagaccctc tcactcacct gtgccatctc cggggacagt      120

gtccctaaga acggtgcatc ttggaactgg atcaggctgt caccatcgcg aggccttgag      180

tggctgggaa ggactcacta cagttccagg tggtatcatg attatgcatt ctttgtgaag      240

agtcgaataa ccatcaacgt agatacatcc gagacccaag tcagtctgca gctggactct      300

gtgactcccg acgacacggg tgtttattac tgtgcaagag aatctcaacg taggggatgg      360

ttcgacctct ggggccaggg aaccctggtc accgtctccc aggaattcta ggatccggtg      420

gcggtggcag cggcggtggt ggttccggag gcggcggttc taattttatg ctgactcagc      480

cccactctgt gtcggagtct ccggggagga cggttacctt ctcctgcacc cgcagcagtg      540

gcggcattgc cagcaactat gtgcagtggt accaacagcg cccgggcagt acccccacca      600

ctgtgatcta tggggatagc caaagaccct ctggagtccc tgatcggttc tctggctcca      660

tcgacagctc ctccaattct gcctccctca ccatctcagg gctgaaggct gaggacgagg      720

ctgactacta ctgtcagtcc tctgatggta gctcttgggt gttcggcgga ggcacccagc      780

tgaccgtcct ctccggaatt ctagaacaaa agcttatttc tgaagaagac ttgtaatagc      840

tcggcggcgg cat                                                         853


<210>  33
<211>  156
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  33

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Ala Ser Ile Ser Ser Ser 
            20                  25                  30          


Ile Thr Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Pro 
        35                  40                  45              


Glu Trp Ile Gly Ser Met Tyr Tyr Ser Gly Arg Thr Tyr Tyr Asn Pro 
    50                  55                  60                  


Ala Leu Lys Ser Arg Val Thr Ile Ser Pro Asp Lys Ser Lys Asn Gln 
65                  70                  75                  80  


Phe Phe Phe Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Gly Pro Thr His Tyr Tyr Asp Asn Ser Gly 
            100                 105                 110         


Pro Ile Pro Ser Asp Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Ile Leu Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Leu Gln Glu Phe 
145                 150                 155     


<210>  34
<211>  781
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  34
actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt       60

gaaggtagat acccatacga cgttccagac tacgctctgc aggctagtgg tggtggtggt      120

tctggtggtg gtggttctgg tggtggtggt tctgctagcc aggtgcagct gcaggagtcg      180

ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt ctctggtgcc      240

tccatcagca gtagtcatta ctactggggc tggatccgcc agcccccagg gaaggggcct      300

gagtggattg ggagtatgta ttatagtggg agaacgtact acaacccggc cctcaagagt      360

cgagtcacca tatcaccaga caagtcgaag aaccagttct tcttgaagtt gacctctgta      420

accgccgcgg acacggccgt gtattactgt gcgagggagg gacccacaca ttactatgat      480

aatagtggtc caataccttc ggatgagtat ttccagcact ggggccaggg taccctggtc      540

actgtctcct caggaattct aggatccggt ggcggtggca gcggcggtgg tggttccgga      600

ggcggcgggc tgcaggaatt ctagaacaaa agcttatttc tgaagaagac ttgtaatagc      660

tcggcggccg catcgagatc tgataacaac agtgtagatg taacaaaatc gactttgttc      720

ccactgtact tttagctcgt acaaaataca atatactttt catttctccg taaacaacat      780

g                                                                      781


<210>  35
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  35

Gln Val Gln Leu Val Glu Ser Glu Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Thr Ile Pro Ile Phe Gly Thr Ala Asp Tyr Ala Gln Glu Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Thr Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Gly Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Leu Leu Gly Thr Thr Met Val Thr Gly His Tyr Phe Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Ile Leu Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asn Phe 
    130                 135                 140                 


Met Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln Ser Val 
145                 150                 155                 160 


Thr Ile Ser Cys Ser Gly Ser Gly Ser Asn Ile Gly Asn Asn Lys Val 
                165                 170                 175     


Asn Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile Tyr 
            180                 185                 190         


Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln Ser Glu 
    210                 215                 220                 


Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Gly Leu Ser Gly 
225                 230                 235                 240 


Tyr Val Phe Gly Thr Gly Thr Lys Leu Thr Val Leu 
                245                 250         


<210>  36
<211>  845
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  36
ggttctggtg gtggtggttc tgctagccag gtgcagctgg tggaatctga ggctgaggtg       60

aagaagcctg ggtcctcggt gaaggtctcc tgcaaggcct ctggaggcac cttcagcagc      120

tatgctatca gctgggtgcg gcaggcccct ggacaagggc ttgagtggat gggagggacc      180

atccctatct ttggtacagc agactacgca caggagttcc agggcagagt cacgattacc      240

acggacgaat ccacgagcac agcctacatg gagctgagcg gcctgagatc tgaggacacg      300

gccgtgtatt actgtgtttt gttgggtaca actatggtta cgggacacta ctttgactac      360

tggggccagg gaaccctggt caccgtctcc tcaggaattc taggatccgg tggcggtggc      420

agcggcggtg gtggttccgg aggcggcggt tctaatttta tgctgactca gcccccctca      480

gcgtctggga cccccgggca gagcgtcacc atctcttgtt ctggaagcgg ctcgaacatc      540

ggaaacaata aagtaaactg gtaccagcag ctcccaggaa cggcccccaa actcctcatc      600

tatagtaata atcagcggcc ctcaggggtc cctgaccgat tctctggctc caagtctggc      660

acctcagcct ccctggccat cagtgggctc cagtctgagg atgaggctga ttattactgt      720

gcagcatggg atgacggtct gagtggttat gtcttcggaa ctgggaccaa gcttaccgtc      780

ctgtccggat ccgaacaaaa gcttatttct gaagaggact tgtaatagct cggcggccgc      840

atcga                                                                  845


<210>  37
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  37

Gln Val Gln Leu Val Glu Ser Glu Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Thr Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Leu Leu Gly Thr Thr Met Val Thr Gly Tyr Tyr Phe Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Ile Leu Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asn Phe 
    130                 135                 140                 


Thr Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln Ser Val 
145                 150                 155                 160 


Thr Ile Ser Cys Ser Gly Ser Gly Ser Asn Ile Gly Asn Asn Lys Val 
                165                 170                 175     


Asn Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile Tyr 
            180                 185                 190         


Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln Ser Glu 
    210                 215                 220                 


Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser Leu Asn Gly 
225                 230                 235                 240 


Tyr Val Phe Gly Thr Gly Thr Lys Leu Thr Val Leu 
                245                 250         


<210>  38
<211>  864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  38
gctctgcagg ctagtggtgg tggtggttct ggtggtggtg gttctggtgg tggtggttct       60

gctagccagg tgcagctggt ggaatctgag gctgaggtga agaagcctgg gtcctcggtg      120

aaggtctcct gcaaggcttc tggaggcacc ttcagcagct atgctatcag ctgggtgcga      180

caggcccctg gacaagggct cgagtggatg ggagggacca tccctatctt tggtacagca      240

aactacgcac agaagttcca gggcagagtc acgattaccg cggacgagtc cacgagcaca      300

gcctacatgg agctgagcag cctgagatct gaggacacgg ccgtgtatta ctgtgtcttg      360

ttgggtacaa ctatggttac gggatactac tttgactact ggggccaggg aaccctggtc      420

accgtctcct caggaattct aggatccggt ggcggtggca gcggcggtgg tggttccgga      480

ggcggcggtt ctaattttac gctgactcag cccccctcag cgtctgggac ccccgggcag      540

agcgtcacca tctcttgttc tggaagcggc tcgaacatcg gaaacaataa agtaaactgg      600

taccagcagc tcccaggaac ggcccccaaa ctcctcatct atagtaataa tcagcggccc      660

tcaggggtcc ctgaccgatt ctctggctcc aagtctggca cctcagcctc cctggccatc      720

agtgggctcc agtctgagga tgaggctgat tattactgtg cagcatggga tgacagcctg      780

aatggttatg tcttcggaac tgggaccaag ctcaccgtcc tatccggaat tctagaacaa      840

aagcttattt ctgaagaaga cttg                                             864


<210>  39
<211>  250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  39

Gln Val Gln Leu Gln Gln Gly Gly Ala Gly Leu Leu Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Gly Val Tyr Gly Gly Ser Phe Ser Gly Tyr 
            20                  25                  30          


Tyr Trp Ser Trp Ile Arg Gln Ser Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn His Ser Gly Ser Ala Asn Tyr Asn Pro Ser Val Lys 
    50                  55                  60                  


Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser Leu 
65                  70                  75                  80  


Gln Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asp Arg Ala Val Leu Thr Gly Glu Gly Trp Tyr Phe Asp Leu Trp 
            100                 105                 110         


Gly Arg Gly Thr Leu Val Thr Val Ser Ser Gly Ile Leu Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Tyr 
    130                 135                 140                 


Glu Leu Thr Gln Pro Pro Ser Val Ser Val Ser Pro Gly Gln Thr Ala 
145                 150                 155                 160 


Ser Ile Thr Cys Ser Gly Asp Lys Leu Gly Asp Lys Tyr Thr Cys Trp 
                165                 170                 175     


Tyr Gln Gln Lys Pro Gly Gln Ser Pro Val Leu Val Leu Tyr Glu Asp 
            180                 185                 190         


Thr Lys Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Ser Asn Ser 
        195                 200                 205             


Gly Asn Thr Ala Thr Leu Thr Ile Ser Arg Val Glu Ala Gly Asp Glu 
    210                 215                 220                 


Ala Asp Tyr Tyr Cys Gln Leu Trp Asp Ser Ser Ser Asp His Tyr Val 
225                 230                 235                 240 


Phe Gly Ser Gly Thr Lys Leu Thr Val Leu 
                245                 250 


<210>  40
<211>  835
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scFv sequence

<400>  40
ctggtggtgg tggttctgct agccaggtgc agctacagca ggggggcgca ggactgttga       60

agccttcgga gaccctgtcc ctcacgtgcg gtgtctatgg tgggtctttc agtggttact      120

attggagctg gattcgccag tccccaggaa aggggctgga atggattggg gaaatcaatc      180

atagtggaag cgccaactac aacccgtccg tcaagagtcg tgtcaccata tcagtagaca      240

cgtccaagaa tcagttctcc ctgcagttga gctctgtgac cgctgcggac acggccgtgt      300

actactgtgc gagagatagg gcggtgttaa cgggggaggg ctggtacttc gatctctggg      360

gccgtggtac cctggtcacc gtctcctcag gaattctagg atccggtggc ggtggcagcg      420

gcggtggtgg ttccggaggc ggcggttctt cctatgagct gacacagcca ccctcagtgt      480

ccgtgtcccc aggacagaca gccagcatca cctgctctgg agataaattg ggggataaat      540

atacttgttg gtatcagcag aagccaggcc agtcccctgt actggtcctc tatgaagata      600

ccaagcggcc ctcagggatc cctgagcgat tctctggctc caactctggg aacacggcca      660

ccctgaccat cagcagggtc gaagccgggg atgaggccga ctattactgt cagctgtggg      720

atagtagtag tgatcattat gtcttcggaa gtgggaccaa gctgaccgtc ctatccggaa      780

ttctagaaca aaagcttatt tcagaagaag acttgtaata gctcggcggc cgcat           835


<210>  41
<211>  270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - furin (short) biosensor (protein)

<400>  41

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Ala Ser Ile Ser Ser Ser 
            20                  25                  30          


Ile Thr Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Pro 
        35                  40                  45              


Glu Trp Ile Gly Ser Met Tyr Tyr Ser Gly Arg Thr Tyr Tyr Asn Pro 
    50                  55                  60                  


Ala Leu Lys Ser Arg Val Thr Ile Ser Pro Asp Lys Ser Lys Asn Gln 
65                  70                  75                  80  


Phe Phe Phe Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Gly Pro Thr His Tyr Tyr Asp Asn Ser Gly 
            100                 105                 110         


Pro Ile Pro Ser Asp Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Ile Leu Gly Ser Arg Lys Lys Arg Ser Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Asn Phe Met Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln 
                165                 170                 175     


Ser Val Thr Ile Ser Cys Ser Gly Ser Gly Ser Asn Ile Gly Asn Asn 
            180                 185                 190         


Lys Val Asn Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        195                 200                 205             


Ile Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser 
    210                 215                 220                 


Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln 
225                 230                 235                 240 


Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser Leu 
                245                 250                 255     


Asn Gly Tyr Val Phe Gly Thr Gly Thr Lys Leu Thr Val Leu 
            260                 265                 270 


<210>  42
<211>  1033
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - furin (short) biosensor (DNA)

<400>  42
actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt       60

gaaggtagat acccatacga cgttccagac tacgctctgc aggctagtgg tggtggtggt      120

tctggtggtg gtggttctgg tggtggtggt tctgctagcc aggtgcagct gcaggagtcg      180

ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt ctctggtgcc      240

tccatcagca gtagtcatta ctactggggc tggatccgcc agcccccagg gaaggggcct      300

gagtggattg ggagtatgta ttatagtggg agaacgtact acaacccggc cctcaagagt      360

cgagtcacca tatcaccaga caagtcgaag aaccagttct tcttgaagtt gacctctgta      420

accgccgcgg acacggccgt gtattactgt gcgagggagg gacccacaca ttactatgat      480

aatagtggtc caataccttc ggatgagtat ttccagcact ggggccaggg taccctggtc      540

actgtctcct caggaattct aggatcgaga aagaagagat ctggatccgg tggcggtggc      600

agcggcggtg gtggttccgg aggcggcggt tctaatttta tgctgactca gcccccctca      660

gcgtctggga cccccgggca gagcgtcacc atctcttgtt ctggaagcgg ctcgaacatc      720

ggaaacaata aagtaaactg gtaccagcag ctcccaggaa cggcccccaa actcctcatc      780

tatagtaata atcagcggcc ctcaggggtc cctgaccgat tctctggctc caagtctggc      840

acctcagcct ccctggccat cagtgggctc cagtctgagg atgaggctga ttattactgt      900

gcagcatggg atgacagcct gaatggttat gtcttcggaa ctgggaccaa gctcaccgtc      960

ctatccggaa ttctagaaca aaagcttatt tctgaagaag acttgtaata gctcggcggc     1020

cgcatcgaga tct                                                        1033


<210>  43
<211>  275
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - furin (long) biosensor (protein)

<400>  43

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Ala Ser Ile Ser Ser Ser 
            20                  25                  30          


Ile Thr Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Pro 
        35                  40                  45              


Glu Trp Ile Gly Ser Met Tyr Tyr Ser Gly Arg Thr Tyr Tyr Asn Pro 
    50                  55                  60                  


Ala Leu Lys Ser Arg Val Thr Ile Ser Pro Asp Lys Ser Lys Asn Gln 
65                  70                  75                  80  


Phe Phe Phe Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Gly Pro Thr His Tyr Tyr Asp Asn Ser Gly 
            100                 105                 110         


Pro Ile Pro Ser Asp Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Ile Leu Gly Ser Asn Ser Arg Lys Lys Arg 
    130                 135                 140                 


Ser Thr Ser Ala Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Asn Phe Met Leu Thr Gln Pro Pro Ser Ala Ser 
                165                 170                 175     


Gly Thr Pro Gly Gln Ser Val Thr Ile Ser Cys Ser Gly Ser Gly Ser 
            180                 185                 190         


Asn Ile Gly Asn Asn Lys Val Asn Trp Tyr Gln Gln Leu Pro Gly Thr 
        195                 200                 205             


Ala Pro Lys Leu Leu Ile Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val 
    210                 215                 220                 


Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala 
225                 230                 235                 240 


Ile Ser Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala 
                245                 250                 255     


Trp Asp Asp Ser Leu Asn Gly Tyr Val Phe Gly Thr Gly Thr Lys Leu 
            260                 265                 270         


Thr Val Leu 
        275 


<210>  44
<211>  1048
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - furin (long) biosensor (DNA)

<400>  44
actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt       60

gaaggtagat acccatacga cgttccagac tacgctctgc aggctagtgg tggtggtggt      120

tctggtggtg gtggttctgg tggtggtggt tctgctagcc aggtgcagct gcaggagtcg      180

ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt ctctggtgcc      240

tccatcagca gtagtcatta ctactggggc tggatccgcc agcccccagg gaaggggcct      300

gagtggattg ggagtatgta ttatagtggg agaacgtact acaacccggc cctcaagagt      360

cgagtcacca tatcaccaga caagtcgaag aaccagttct tcttgaagtt gacctctgta      420

accgccgcgg acacggccgt gtattactgt gcgagggagg gacccacaca ttactatgat      480

aatagtggtc caataccttc ggatgagtat ttccagcact ggggccaggg taccctggtc      540

actgtctcct caggaattct aggatcgaac tcgagaaaga agagatctac ctccgctgga      600

tccggtggcg gtggcagcgg cggtggtggt tccggaggcg gcggttctaa ttttatgctg      660

actcagcccc cctcagcgtc tgggaccccc gggcagagcg tcaccatctc ttgttctgga      720

agcggctcga acatcggaaa caataaagta aactggtacc agcagctccc aggaacggcc      780

cccaaactcc tcatctatag taataatcag cggccctcag gggtccctga ccgattctct      840

ggctccaagt ctggcacctc agcctccctg gccatcagtg ggctccagtc tgaggatgag      900

gctgattatt actgtgcagc atgggatgac agcctgaatg gttatgtctt cggaactggg      960

accaagctca ccgtcctatc cggaattcta gaacaaaagc ttatttctga agaagacttg     1020

taatagctcg gcggccgcat cgagatct                                        1048


<210>  45
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - TEV protease biosensor (protein)

<400>  45

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Ala Ser Ile Ser Ser Ser 
            20                  25                  30          


Ile Thr Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Pro 
        35                  40                  45              


Glu Trp Ile Gly Ser Met Tyr Tyr Ser Gly Arg Thr Tyr Tyr Asn Pro 
    50                  55                  60                  


Ala Leu Lys Ser Arg Val Thr Ile Ser Pro Asp Lys Ser Lys Asn Gln 
65                  70                  75                  80  


Phe Phe Phe Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Gly Pro Thr His Tyr Tyr Asp Asn Ser Gly 
            100                 105                 110         


Pro Ile Pro Ser Asp Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Ile Leu Gly Ser Glu Asn Leu Tyr Phe Glu 
    130                 135                 140                 


Gly Arg Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Asn Phe Met Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro 
                165                 170                 175     


Gly Gln Ser Val Thr Ile Ser Cys Ser Gly Ser Gly Ser Asn Ile Gly 
            180                 185                 190         


Asn Asn Lys Val Asn Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys 
        195                 200                 205             


Leu Leu Ile Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg 
    210                 215                 220                 


Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly 
225                 230                 235                 240 


Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp 
                245                 250                 255     


Ser Leu Asn Gly Tyr Val Phe Gly Thr Gly Thr Lys Leu Thr Val Leu 
            260                 265                 270         


<210>  46
<211>  1039
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - TEV protease biosensor (DNA)

<400>  46
actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt       60

gaaggtagat acccatacga cgttccagac tacgctctgc aggctagtgg tggtggtggt      120

tctggtggtg gtggttctgg tggtggtggt tctgctagcc aggtgcagct gcaggagtcg      180

ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt ctctggtgcc      240

tccatcagca gtagtcatta ctactggggc tggatccgcc agcccccagg gaaggggcct      300

gagtggattg ggagtatgta ttatagtggg agaacgtact acaacccggc cctcaagagt      360

cgagtcacca tatcaccaga caagtcgaag aaccagttct tcttgaagtt gacctctgta      420

accgccgcgg acacggccgt gtattactgt gcgagggagg gacccacaca ttactatgat      480

aatagtggtc caataccttc ggatgagtat ttccagcact ggggccaggg taccctggtc      540

actgtctcct caggaattct aggatctgaa aacctatact tccaaggtcg atccggtggc      600

ggtggcagcg gcggtggtgg ttccggaggc ggcggttcta attttatgct gactcagccc      660

ccctcagcgt ctgggacccc cgggcagagc gtcaccatct cttgttctgg aagcggctcg      720

aacatcggaa acaataaagt aaactggtac cagcagctcc caggaacggc ccccaaactc      780

ctcatctata gtaataatca gcggccctca ggggtccctg accgattctc tggctccaag      840

tctggcacct cagcctccct ggccatcagt gggctccagt ctgaggatga ggctgattat      900

tactgtgcag catgggatga cagcctgaat ggttatgtct tcggaactgg gaccaagctc      960

accgtcctat ccggaattct agaacaaaag cttatttctg aagaagactt gtaatagctc     1020

ggcggccgca tcgagatct                                                  1039


<210>  47
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - HRV-3C protease biosensor (protein)

<400>  47

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Ala Ser Ile Ser Ser Ser 
            20                  25                  30          


Ile Thr Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Pro 
        35                  40                  45              


Glu Trp Ile Gly Ser Met Tyr Tyr Ser Gly Arg Thr Tyr Tyr Asn Pro 
    50                  55                  60                  


Ala Leu Lys Ser Arg Val Thr Ile Ser Pro Asp Lys Ser Lys Asn Gln 
65                  70                  75                  80  


Phe Phe Phe Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Gly Pro Thr His Tyr Tyr Asp Asn Ser Gly 
            100                 105                 110         


Pro Ile Pro Ser Asp Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Ile Leu Gly Ser Leu Glu Val Leu Phe Gln 
    130                 135                 140                 


Gly Pro Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
145                 150                 155                 160 


Gly Gly Ser Asn Phe Met Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr 
                165                 170                 175     


Pro Gly Gln Ser Val Thr Ile Ser Cys Ser Gly Ser Gly Ser Asn Ile 
            180                 185                 190         


Gly Asn Asn Lys Val Asn Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro 
        195                 200                 205             


Lys Leu Leu Ile Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp 
    210                 215                 220                 


Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser 
225                 230                 235                 240 


Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp 
                245                 250                 255     


Asp Ser Leu Asn Gly Tyr Val Phe Gly Thr Gly Thr Lys Leu Thr Val 
            260                 265                 270         


Leu 
    


<210>  48
<211>  1042
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - HRV-3C protease biosensor (DNA)

<400>  48
actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt       60

gaaggtagat acccatacga cgttccagac tacgctctgc aggctagtgg tggtggtggt      120

tctggtggtg gtggttctgg tggtggtggt tctgctagcc aggtgcagct gcaggagtcg      180

ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt ctctggtgcc      240

tccatcagca gtagtcatta ctactggggc tggatccgcc agcccccagg gaaggggcct      300

gagtggattg ggagtatgta ttatagtggg agaacgtact acaacccggc cctcaagagt      360

cgagtcacca tatcaccaga caagtcgaag aaccagttct tcttgaagtt gacctctgta      420

accgccgcgg acacggccgt gtattactgt gcgagggagg gacccacaca ttactatgat      480

aatagtggtc caataccttc ggatgagtat ttccagcact ggggccaggg taccctggtc      540

actgtctcct caggaattct aggatctttg gaagttttgt tccaaggtcc aggatccggt      600

ggcggtggca gcggcggtgg tggttccgga ggcggcggtt ctaattttat gctgactcag      660

cccccctcag cgtctgggac ccccgggcag agcgtcacca tctcttgttc tggaagcggc      720

tcgaacatcg gaaacaataa agtaaactgg taccagcagc tcccaggaac ggcccccaaa      780

ctcctcatct atagtaataa tcagcggccc tcaggggtcc ctgaccgatt ctctggctcc      840

aagtctggca cctcagcctc cctggccatc agtgggctcc agtctgagga tgaggctgat      900

tattactgtg cagcatggga tgacagcctg aatggttatg tcttcggaac tgggaccaag      960

ctcaccgtcc tatccggaat tctagaacaa aagcttattt ctgaagaaga cttgtaatag     1020

ctcggcggcc gcatcgagat ct                                              1042


<210>  49
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - Caspase 1 biosensor (protein)

<400>  49

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Ala Ser Ile Ser Ser Ser 
            20                  25                  30          


Ile Thr Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Pro 
        35                  40                  45              


Glu Trp Ile Gly Ser Met Tyr Tyr Ser Gly Arg Thr Tyr Tyr Asn Pro 
    50                  55                  60                  


Ala Leu Lys Ser Arg Val Thr Ile Ser Pro Asp Lys Ser Lys Asn Gln 
65                  70                  75                  80  


Phe Phe Phe Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Gly Pro Thr His Tyr Tyr Asp Asn Ser Gly 
            100                 105                 110         


Pro Ile Pro Ser Asp Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Ile Leu Gly Ser Tyr Val Ala Asp Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asn 
145                 150                 155                 160 


Phe Met Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln Ser 
                165                 170                 175     


Val Thr Ile Ser Cys Ser Gly Ser Gly Ser Asn Ile Gly Asn Asn Lys 
            180                 185                 190         


Val Asn Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly 
    210                 215                 220                 


Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln Ser 
225                 230                 235                 240 


Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser Leu Asn 
                245                 250                 255     


Gly Tyr Val Phe Gly Thr Gly Thr Lys Leu Thr Val Leu 
            260                 265                 


<210>  50
<211>  1030
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - Caspase 1 biosensor (DNA)

<400>  50
actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt       60

gaaggtagat acccatacga cgttccagac tacgctctgc aggctagtgg tggtggtggt      120

tctggtggtg gtggttctgg tggtggtggt tctgctagcc aggtgcagct gcaggagtcg      180

ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt ctctggtgcc      240

tccatcagca gtagtcatta ctactggggc tggatccgcc agcccccagg gaaggggcct      300

gagtggattg ggagtatgta ttatagtggg agaacgtact acaacccggc cctcaagagt      360

cgagtcacca tatcaccaga caagtcgaag aaccagttct tcttgaagtt gacctctgta      420

accgccgcgg acacggccgt gtattactgt gcgagggagg gacccacaca ttactatgat      480

aatagtggtc caataccttc ggatgagtat ttccagcact ggggccaggg taccctggtc      540

actgtctcct caggaattct aggatcttac gttgctgacg gatccggtgg cggtggcagc      600

ggcggtggtg gttccggagg cggcggttct aattttatgc tgactcagcc cccctcagcg      660

tctgggaccc ccgggcagag cgtcaccatc tcttgttctg gaagcggctc gaacatcgga      720

aacaataaag taaactggta ccagcagctc ccaggaacgg cccccaaact cctcatctat      780

agtaataatc agcggccctc aggggtccct gaccgattct ctggctccaa gtctggcacc      840

tcagcctccc tggccatcag tgggctccag tctgaggatg aggctgatta ttactgtgca      900

gcatgggatg acagcctgaa tggttatgtc ttcggaactg ggaccaagct caccgtccta      960

tccggaattc tagaacaaaa gcttatttct gaagaagact tgtaatagct cggcggccgc     1020

atcgagatct                                                            1030


<210>  51
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - Caspase 3 biosensor (protein)

<400>  51

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Ala Ser Ile Ser Ser Ser 
            20                  25                  30          


Ile Thr Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Pro 
        35                  40                  45              


Glu Trp Ile Gly Ser Met Tyr Tyr Ser Gly Arg Thr Tyr Tyr Asn Pro 
    50                  55                  60                  


Ala Leu Lys Ser Arg Val Thr Ile Ser Pro Asp Lys Ser Lys Asn Gln 
65                  70                  75                  80  


Phe Phe Phe Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Gly Pro Thr His Tyr Tyr Asp Asn Ser Gly 
            100                 105                 110         


Pro Ile Pro Ser Asp Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Ile Leu Gly Ser Asp Glu Val Asp Arg Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asn 
145                 150                 155                 160 


Phe Met Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln Ser 
                165                 170                 175     


Val Thr Ile Ser Cys Ser Gly Ser Gly Ser Asn Ile Gly Asn Asn Lys 
            180                 185                 190         


Val Asn Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly 
    210                 215                 220                 


Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln Ser 
225                 230                 235                 240 


Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser Leu Asn 
                245                 250                 255     


Gly Tyr Val Phe Gly Thr Gly Thr Lys Leu Thr Val Leu 
            260                 265                 


<210>  52
<211>  1030
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - Caspase 3 biosensor (DNA)

<400>  52
actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt       60

gaaggtagat acccatacga cgttccagac tacgctctgc aggctagtgg tggtggtggt      120

tctggtggtg gtggttctgg tggtggtggt tctgctagcc aggtgcagct gcaggagtcg      180

ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt ctctggtgcc      240

tccatcagca gtagtcatta ctactggggc tggatccgcc agcccccagg gaaggggcct      300

gagtggattg ggagtatgta ttatagtggg agaacgtact acaacccggc cctcaagagt      360

cgagtcacca tatcaccaga caagtcgaag aaccagttct tcttgaagtt gacctctgta      420

accgccgcgg acacggccgt gtattactgt gcgagggagg gacccacaca ttactatgat      480

aatagtggtc caataccttc ggatgagtat ttccagcact ggggccaggg taccctggtc      540

actgtctcct caggaattct aggatctgac gaagttgacc gatccggtgg cggtggcagc      600

ggcggtggtg gttccggagg cggcggttct aattttatgc tgactcagcc cccctcagcg      660

tctgggaccc ccgggcagag cgtcaccatc tcttgttctg gaagcggctc gaacatcgga      720

aacaataaag taaactggta ccagcagctc ccaggaacgg cccccaaact cctcatctat      780

agtaataatc agcggccctc aggggtccct gaccgattct ctggctccaa gtctggcacc      840

tcagcctccc tggccatcag tgggctccag tctgaggatg aggctgatta ttactgtgca      900

gcatgggatg acagcctgaa tggttatgtc ttcggaactg ggaccaagct caccgtccta      960

tccggaattc tagaacaaaa gcttatttct gaagaagact tgtaatagct cggcggccgc     1020

atcgagatct                                                            1030


<210>  53
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - MMP25 biosensor (protein)

<400>  53

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Ala Ser Ile Ser Ser Ser 
            20                  25                  30          


Ile Thr Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Pro 
        35                  40                  45              


Glu Trp Ile Gly Ser Met Tyr Tyr Ser Gly Arg Thr Tyr Tyr Asn Pro 
    50                  55                  60                  


Ala Leu Lys Ser Arg Val Thr Ile Ser Pro Asp Lys Ser Lys Asn Gln 
65                  70                  75                  80  


Phe Phe Phe Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Gly Pro Thr His Tyr Tyr Asp Asn Ser Gly 
            100                 105                 110         


Pro Ile Pro Ser Asp Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Ile Leu Gly Ser Val Met Arg Leu Val Val 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Asn Phe Met Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly 
                165                 170                 175     


Gln Ser Val Thr Ile Ser Cys Ser Gly Ser Gly Ser Asn Ile Gly Asn 
            180                 185                 190         


Asn Lys Val Asn Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu 
        195                 200                 205             


Leu Ile Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe 
    210                 215                 220                 


Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu 
225                 230                 235                 240 


Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser 
                245                 250                 255     


Leu Asn Gly Tyr Val Phe Gly Thr Gly Thr Lys Leu Thr Val Leu 
            260                 265                 270     


<210>  54
<211>  1036
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - MMP25 biosensor (DNA)

<400>  54
actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt       60

gaaggtagat acccatacga cgttccagac tacgctctgc aggctagtgg tggtggtggt      120

tctggtggtg gtggttctgg tggtggtggt tctgctagcc aggtgcagct gcaggagtcg      180

ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt ctctggtgcc      240

tccatcagca gtagtcatta ctactggggc tggatccgcc agcccccagg gaaggggcct      300

gagtggattg ggagtatgta ttatagtggg agaacgtact acaacccggc cctcaagagt      360

cgagtcacca tatcaccaga caagtcgaag aaccagttct tcttgaagtt gacctctgta      420

accgccgcgg acacggccgt gtattactgt gcgagggagg gacccacaca ttactatgat      480

aatagtggtc caataccttc ggatgagtat ttccagcact ggggccaggg taccctggtc      540

actgtctcct caggaattct aggatcggtc atgagattgg tcgtcggatc cggtggcggt      600

ggcagcggcg gtggtggttc cggaggcggc ggttctaatt ttatgctgac tcagcccccc      660

tcagcgtctg ggacccccgg gcagagcgtc accatctctt gttctggaag cggctcgaac      720

atcggaaaca ataaagtaaa ctggtaccag cagctcccag gaacggcccc caaactcctc      780

atctatagta ataatcagcg gccctcaggg gtccctgacc gattctctgg ctccaagtct      840

ggcacctcag cctccctggc catcagtggg ctccagtctg aggatgaggc tgattattac      900

tgtgcagcat gggatgacag cctgaatggt tatgtcttcg gaactgggac caagctcacc      960

gtcctatccg gaattctaga acaaaagctt atttctgaag aagacttgta atagctcggc     1020

ggccgcatcg agatct                                                     1036


<210>  55
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - PKA biosensor (protein)

<400>  55

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Ala Ser Ile Ser Ser Ser 
            20                  25                  30          


Ile Thr Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Pro 
        35                  40                  45              


Glu Trp Ile Gly Ser Met Tyr Tyr Ser Gly Arg Thr Tyr Tyr Asn Pro 
    50                  55                  60                  


Ala Leu Lys Ser Arg Val Thr Ile Ser Pro Asp Lys Ser Lys Asn Gln 
65                  70                  75                  80  


Phe Phe Phe Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Gly Pro Thr His Tyr Tyr Asp Asn Ser Gly 
            100                 105                 110         


Pro Ile Pro Ser Asp Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Ile Leu Gly Ser Leu Leu Arg Arg Ala Ser 
    130                 135                 140                 


Leu Gly Pro Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
145                 150                 155                 160 


Gly Gly Gly Ser Asn Phe Met Leu Thr Gln Pro Pro Ser Ala Ser Gly 
                165                 170                 175     


Thr Pro Gly Gln Ser Val Thr Ile Ser Cys Ser Gly Ser Gly Ser Asn 
            180                 185                 190         


Ile Gly Asn Asn Lys Val Asn Trp Tyr Gln Gln Leu Pro Gly Thr Ala 
        195                 200                 205             


Pro Lys Leu Leu Ile Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val Pro 
    210                 215                 220                 


Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile 
225                 230                 235                 240 


Ser Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp 
                245                 250                 255     


Asp Asp Ser Leu Asn Gly Tyr Val Phe Gly Thr Gly Thr Lys Leu Thr 
            260                 265                 270         


Val Leu 
        


<210>  56
<211>  1045
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - PKA biosensor (DNA)

<400>  56
actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt       60

gaaggtagat acccatacga cgttccagac tacgctctgc aggctagtgg tggtggtggt      120

tctggtggtg gtggttctgg tggtggtggt tctgctagcc aggtgcagct gcaggagtcg      180

ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt ctctggtgcc      240

tccatcagca gtagtcatta ctactggggc tggatccgcc agcccccagg gaaggggcct      300

gagtggattg ggagtatgta ttatagtggg agaacgtact acaacccggc cctcaagagt      360

cgagtcacca tatcaccaga caagtcgaag aaccagttct tcttgaagtt gacctctgta      420

accgccgcgg acacggccgt gtattactgt gcgagggagg gacccacaca ttactatgat      480

aatagtggtc caataccttc ggatgagtat ttccagcact ggggccaggg taccctggtc      540

actgtctcct caggaattct aggatcgttg ttgagaagag ctagtttggg tccaggatcc      600

ggtggcggtg gcagcggcgg tggtggttcc ggaggcggcg gttctaattt tatgctgact      660

cagcccccct cagcgtctgg gacccccggg cagagcgtca ccatctcttg ttctggaagc      720

ggctcgaaca tcggaaacaa taaagtaaac tggtaccagc agctcccagg aacggccccc      780

aaactcctca tctatagtaa taatcagcgg ccctcagggg tccctgaccg attctctggc      840

tccaagtctg gcacctcagc ctccctggcc atcagtgggc tccagtctga ggatgaggct      900

gattattact gtgcagcatg ggatgacagc ctgaatggtt atgtcttcgg aactgggacc      960

aagctcaccg tcctatccgg aattctagaa caaaagctta tttctgaaga agacttgtaa     1020

tagctcggcg gccgcatcga gatct                                           1045


<210>  57
<211>  276
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - H3-K56 HAT biosensor (protein)

<400>  57

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Ala Ser Ile Ser Ser Ser 
            20                  25                  30          


Ile Thr Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Pro 
        35                  40                  45              


Glu Trp Ile Gly Ser Met Tyr Tyr Ser Gly Arg Thr Tyr Tyr Asn Pro 
    50                  55                  60                  


Ala Leu Lys Ser Arg Val Thr Ile Ser Pro Asp Lys Ser Lys Asn Gln 
65                  70                  75                  80  


Phe Phe Phe Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Glu Gly Pro Thr His Tyr Tyr Asp Asn Ser Gly 
            100                 105                 110         


Pro Ile Pro Ser Asp Glu Tyr Phe Gln His Trp Gly Gln Gly Thr Leu 
        115                 120                 125             


Val Thr Val Ser Ser Gly Ile Leu Gly Ser Ile Arg Arg Phe Gln Lys 
    130                 135                 140                 


Ser Thr Asp Leu Leu Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Gly Gly Gly Gly Ser Asn Phe Met Leu Thr Gln Pro Pro Ser Ala 
                165                 170                 175     


Ser Gly Thr Pro Gly Gln Ser Val Thr Ile Ser Cys Ser Gly Ser Gly 
            180                 185                 190         


Ser Asn Ile Gly Asn Asn Lys Val Asn Trp Tyr Gln Gln Leu Pro Gly 
        195                 200                 205             


Thr Ala Pro Lys Leu Leu Ile Tyr Ser Asn Asn Gln Arg Pro Ser Gly 
    210                 215                 220                 


Val Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu 
225                 230                 235                 240 


Ala Ile Ser Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala 
                245                 250                 255     


Ala Trp Asp Asp Ser Leu Asn Gly Tyr Val Phe Gly Thr Gly Thr Lys 
            260                 265                 270         


Leu Thr Val Leu 
        275     


<210>  58
<211>  1051
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H6-MG (VH) / HL1-TO1 (VL) - H3-K56 HAT biosensor (DNA)

<400>  58
actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt       60

gaaggtagat acccatacga cgttccagac tacgctctgc aggctagtgg tggtggtggt      120

tctggtggtg gtggttctgg tggtggtggt tctgctagcc aggtgcagct gcaggagtcg      180

ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt ctctggtgcc      240

tccatcagca gtagtcatta ctactggggc tggatccgcc agcccccagg gaaggggcct      300

gagtggattg ggagtatgta ttatagtggg agaacgtact acaacccggc cctcaagagt      360

cgagtcacca tatcaccaga caagtcgaag aaccagttct tcttgaagtt gacctctgta      420

accgccgcgg acacggccgt gtattactgt gcgagggagg gacccacaca ttactatgat      480

aatagtggtc caataccttc ggatgagtat ttccagcact ggggccaggg taccctggtc      540

actgtctcct caggaattct aggatcgatc agaagattcc aaaagtccac cgacttgttg      600

ggatccggtg gcggtggcag cggcggtggt ggttccggag gcggcggttc taattttatg      660

ctgactcagc ccccctcagc gtctgggacc cccgggcaga gcgtcaccat ctcttgttct      720

ggaagcggct cgaacatcgg aaacaataaa gtaaactggt accagcagct cccaggaacg      780

gcccccaaac tcctcatcta tagtaataat cagcggccct caggggtccc tgaccgattc      840

tctggctcca agtctggcac ctcagcctcc ctggccatca gtgggctcca gtctgaggat      900

gaggctgatt attactgtgc agcatgggat gacagcctga atggttatgt cttcggaact      960

gggaccaagc tcaccgtcct atccggaatt ctagaacaaa agcttatttc tgaagaagac     1020

ttgtaatagc tcggcggccg catcgagatc t                                    1051


