                         SEQUENCE LISTING

<110>  Scarab Genomics LLC
 
<120>  Lac Expression System

<130>  051159.0700.01PC00

<150>  US 60/991,608
<151>  2007-11-30

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Binding site

<400>  1
aattccacat gtggaattgt gagcggatca caatttgtgg                             40


<210>  2
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Binding site

<400>  2
aattccacat gtggaattgt gagcgctcac aattccacat gtgg                        44


<210>  3
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Binding site

<400>  3
tgtgtggaat tgtgagcgct cacaattcca caca                                   34


<210>  4
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Binding site

<400>  4
aattgtgagc gctcacaatt ccacaca                                           27


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Binding site

<400>  5
aattgtgagc gctcacaatt                                                   20


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Binding site

<400>  6
attgtgagcg ctcacaat                                                     18


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Binding site

<400>  7
aattgtaagc gcttacaatt                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Binding site

<400>  8
aattgttagc gctaacaatt                                                   20


<210>  9
<211>  360
<212>  PRT
<213>  Escherichia coli

<400>  9

Met Lys Pro Val Thr Leu Tyr Asp Val Ala Glu Tyr Ala Gly Val Ser 
1               5                   10                  15      


Tyr Gln Thr Val Ser Arg Val Val Asn Gln Ala Ser His Val Ser Ala 
            20                  25                  30          


Lys Thr Arg Glu Lys Val Glu Ala Ala Met Ala Glu Leu Asn Tyr Ile 
        35                  40                  45              


Pro Asn Arg Val Ala Gln Gln Leu Ala Gly Lys Gln Ser Leu Leu Ile 
    50                  55                  60                  


Gly Val Ala Thr Ser Ser Leu Ala Leu His Ala Pro Ser Gln Ile Val 
65                  70                  75                  80  


Ala Ala Ile Lys Ser Arg Ala Asp Gln Leu Gly Ala Ser Val Val Val 
                85                  90                  95      


Ser Met Val Glu Arg Ser Gly Val Glu Ala Cys Lys Ala Ala Val His 
            100                 105                 110         


Asn Leu Leu Ala Gln Arg Val Ser Gly Leu Ile Ile Asn Tyr Pro Leu 
        115                 120                 125             


Asp Asp Gln Asp Ala Ile Ala Val Glu Ala Ala Cys Thr Asn Val Pro 
    130                 135                 140                 


Ala Leu Phe Leu Asp Val Ser Asp Gln Thr Pro Ile Asn Ser Ile Ile 
145                 150                 155                 160 


Phe Ser His Glu Asp Gly Thr Arg Leu Gly Val Glu His Leu Val Ala 
                165                 170                 175     


Leu Gly His Gln Gln Ile Ala Leu Leu Ala Gly Pro Leu Ser Ser Val 
            180                 185                 190         


Ser Ala Arg Leu Arg Leu Ala Gly Trp His Lys Tyr Leu Thr Arg Asn 
        195                 200                 205             


Gln Ile Gln Pro Ile Ala Glu Arg Glu Gly Asp Trp Ser Ala Met Ser 
    210                 215                 220                 


Gly Phe Gln Gln Thr Met Gln Met Leu Asn Glu Gly Ile Val Pro Thr 
225                 230                 235                 240 


Ala Met Leu Val Ala Asn Asp Gln Met Ala Leu Gly Ala Met Arg Ala 
                245                 250                 255     


Ile Thr Glu Ser Gly Leu Arg Val Gly Ala Asp Ile Ser Val Val Gly 
            260                 265                 270         


Tyr Asp Asp Thr Glu Asp Ser Ser Cys Tyr Ile Pro Pro Ser Thr Thr 
        275                 280                 285             


Ile Lys Gln Asp Phe Arg Leu Leu Gly Gln Thr Ser Val Asp Arg Leu 
    290                 295                 300                 


Leu Gln Leu Ser Gln Gly Gln Ala Val Lys Gly Asn Gln Leu Leu Pro 
305                 310                 315                 320 


Val Ser Leu Val Lys Arg Lys Thr Thr Leu Ala Pro Asn Thr Gln Thr 
                325                 330                 335     


Ala Ser Pro Arg Ala Leu Ala Asp Ser Leu Met Gln Leu Ala Arg Gln 
            340                 345                 350         


Val Ser Arg Leu Glu Ser Gly Gln 
        355                 360 


<210>  10
<211>  10
<212>  PRT
<213>  Escherichia coli

<400>  10

Tyr Gln Thr Val Ser Arg Val Val Asn Gln 
1               5                   10  


<210>  11
<211>  10
<212>  PRT
<213>  Escherichia coli

<400>  11

His Gln Thr Val Ser Arg Val Val Asn Gln 
1               5                   10  


<210>  12
<211>  10
<212>  PRT
<213>  Escherichia coli

<400>  12

Tyr Met Thr Val Ser Arg Val Val Asn Gln 
1               5                   10  


<210>  13
<211>  10
<212>  PRT
<213>  Escherichia coli

<400>  13

Ala Gln Thr Val Ser Arg Val Val Asn Gln 
1               5                   10  


<210>  14
<211>  10
<212>  PRT
<213>  Escherichia coli

<400>  14

Val Ala Thr Val Ser Arg Val Val Asn Gln 
1               5                   10  


<210>  15
<211>  10
<212>  PRT
<213>  Escherichia coli

<400>  15

Ala Ala Thr Val Ser Arg Val Val Asn Gln 
1               5                   10  


<210>  16
<211>  10
<212>  PRT
<213>  Escherichia coli

<400>  16

Gln Met Thr Val Ser Arg Val Val Asn Gln 
1               5                   10  


<210>  17
<211>  55
<212>  PRT
<213>  Escherichia coli

<400>  17

Cys Gly Gly Cys Thr Cys Gly Thr Ala Thr Gly Thr Thr Gly Thr Gly 
1               5                   10                  15      


Thr Gly Gly Ala Ala Thr Thr Gly Thr Gly Ala Gly Cys Gly Gly Ala 
            20                  25                  30          


Thr Ala Ala Cys Ala Ala Thr Thr Thr Cys Ala Cys Ala Cys Ala Gly 
        35                  40                  45              


Gly Ala Ala Ala Cys Ala Gly 
    50                  55  


<210>  18
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Binding site

<400>  18

Thr Gly Thr Gly Gly Ala Ala Thr Thr Gly Thr Gly Ala Gly Cys Gly 
1               5                   10                  15      


Cys Thr Cys Ala Cys Ala Ala Thr Thr Cys Cys Ala Cys Ala 
            20                  25                  30  


<210>  19
<211>  360
<212>  PRT
<213>  Escherichia coli

<400>  19

Met Lys Pro Val Thr Leu Tyr Asp Val Ala Glu Tyr Ala Gly Val Ser 
1               5                   10                  15      


Tyr Gln Thr Val Ser Arg Val Val Asn Gln Ala Ser His Val Ser Ala 
            20                  25                  30          


Lys Thr Arg Glu Lys Val Glu Ala Ala Met Ala Glu Leu Asn Tyr Ile 
        35                  40                  45              


Pro Asn Arg Val Ala Gln Gln Leu Ala Gly Lys Gln Ser Leu Leu Ile 
    50                  55                  60                  


Gly Val Ala Thr Ser Ser Leu Ala Leu His Ala Pro Ser Gln Ile Val 
65                  70                  75                  80  


Ala Ala Ile Lys Ser Arg Ala Asp Gln Leu Gly Ala Ser Val Val Val 
                85                  90                  95      


Ser Met Val Glu Arg Ser Gly Val Glu Ala Cys Lys Ala Ala Val His 
            100                 105                 110         


Asn Leu Leu Ala Gln Arg Val Ser Gly Leu Ile Ile Asn Tyr Pro Leu 
        115                 120                 125             


Asp Asp Gln Asp Ala Ile Ala Val Glu Ala Ala Cys Thr Asn Val Pro 
    130                 135                 140                 


Ala Leu Phe Leu Asp Val Ser Asp Gln Thr Pro Ile Asn Ser Ile Ile 
145                 150                 155                 160 


Phe Ser His Glu Asp Gly Thr Arg Leu Gly Val Glu His Leu Val Ala 
                165                 170                 175     


Leu Gly His Gln Gln Ile Ala Leu Leu Ala Gly Pro Leu Ser Ser Val 
            180                 185                 190         


Ser Ala Arg Leu Arg Leu Ala Gly Trp His Lys Tyr Leu Thr Arg Asn 
        195                 200                 205             


Gln Ile Gln Pro Ile Ala Glu Arg Glu Gly Asp Trp Ser Ala Met Ser 
    210                 215                 220                 


Gly Phe Gln Gln Thr Met Gln Met Leu Asn Glu Gly Ile Val Pro Thr 
225                 230                 235                 240 


Ala Met Leu Val Ala Asn Asp Gln Met Ala Leu Gly Ala Met Arg Ala 
                245                 250                 255     


Ile Thr Glu Ser Gly Leu Arg Val Gly Ala Asp Ile Ser Val Val Gly 
            260                 265                 270         


Tyr Asp Asp Thr Glu Asp Ser Ser Cys Tyr Ile Pro Pro Ser Thr Thr 
        275                 280                 285             


Ile Lys Gln Asp Phe Arg Leu Leu Gly Gln Thr Ser Val Asp Arg Leu 
    290                 295                 300                 


Leu Gln Leu Ser Gln Gly Gln Ala Val Lys Gly Asn Gln Leu Leu Pro 
305                 310                 315                 320 


Val Ser Leu Val Lys Arg Lys Thr Thr Leu Ala Pro Asn Thr Gln Thr 
                325                 330                 335     


Ala Ser Pro Arg Ala Leu Ala Asp Ser Leu Met Gln Leu Ala Arg Gln 
            340                 345                 350         


Val Ser Arg Leu Glu Ser Gly Gln 
        355                 360 


<210>  20
<211>  353
<212>  PRT
<213>  Escherichia coli

<400>  20

Met Ala Ile Asp Glu Asn Lys Gln Lys Ala Leu Ala Ala Ala Leu Gly 
1               5                   10                  15      


Gln Ile Glu Lys Gln Phe Gly Lys Gly Ser Ile Met Arg Leu Gly Glu 
            20                  25                  30          


Asp Arg Ser Met Asp Val Glu Thr Ile Ser Thr Gly Ser Leu Ser Leu 
        35                  40                  45              


Asp Ile Ala Leu Gly Ala Gly Gly Leu Pro Met Gly Arg Ile Val Glu 
    50                  55                  60                  


Ile Tyr Gly Pro Glu Ser Ser Gly Lys Thr Thr Leu Thr Leu Gln Val 
65                  70                  75                  80  


Ile Ala Ala Ala Gln Arg Glu Gly Lys Thr Cys Ala Phe Ile Asp Ala 
                85                  90                  95      


Glu His Ala Leu Asp Pro Ile Tyr Ala Arg Lys Leu Gly Val Asp Ile 
            100                 105                 110         


Asp Asn Leu Leu Cys Ser Gln Pro Asp Thr Gly Glu Gln Ala Leu Glu 
        115                 120                 125             


Ile Cys Asp Ala Leu Ala Arg Ser Gly Ala Val Asp Val Ile Val Val 
    130                 135                 140                 


Asp Ser Val Ala Ala Leu Thr Pro Lys Ala Glu Ile Glu Gly Glu Ile 
145                 150                 155                 160 


Gly Asp Ser His Met Gly Leu Ala Ala Arg Met Met Ser Gln Ala Met 
                165                 170                 175     


Arg Lys Leu Ala Gly Asn Leu Lys Gln Ser Asn Thr Leu Leu Ile Phe 
            180                 185                 190         


Ile Asn Gln Ile Arg Met Lys Ile Gly Val Met Phe Gly Asn Pro Glu 
        195                 200                 205             


Thr Thr Thr Gly Gly Asn Ala Leu Lys Phe Tyr Ala Ser Val Arg Leu 
    210                 215                 220                 


Asp Ile Arg Arg Ile Gly Ala Val Lys Glu Gly Glu Asn Val Val Gly 
225                 230                 235                 240 


Ser Glu Thr Arg Val Lys Val Val Lys Asn Lys Ile Ala Ala Pro Phe 
                245                 250                 255     


Lys Gln Ala Glu Phe Gln Ile Leu Tyr Gly Glu Gly Ile Asn Phe Tyr 
            260                 265                 270         


Gly Glu Leu Val Asp Leu Gly Val Lys Glu Lys Leu Ile Glu Lys Ala 
        275                 280                 285             


Gly Ala Trp Tyr Ser Tyr Lys Gly Glu Lys Ile Gly Gln Gly Lys Ala 
    290                 295                 300                 


Asn Ala Thr Ala Trp Leu Lys Asp Asn Pro Glu Thr Ala Lys Glu Ile 
305                 310                 315                 320 


Glu Lys Lys Val Arg Glu Leu Leu Leu Ser Asn Pro Asn Ser Thr Pro 
                325                 330                 335     


Asp Phe Ser Val Asp Asp Ser Glu Gly Val Ala Glu Thr Asn Glu Asp 
            340                 345                 350         


Phe 
    


<210>  21
<211>  1391
<212>  DNA
<213>  Escherichia coli

<400>  21
agagaagcct gtcggcaccg tctggtttgc ttttgccact gcccgcggtg aaggcattac       60

ccggcgggat gcttcagcgg cgaccgtgat gcggtgcgtc gtcaggctac tgcgtatgca      120

ttgcagacct tgtggcaaca atttctacaa aacacttgat actgtatgag catacagtat      180

aattgcttca acagaacata ttgactatcc ggtattaccc ggcatgacag gagtaaaaat      240

ggctatcgac gaaaacaaac agaaagcgtt ggcggcagca ctgggccaga ttgagaaaca      300

atttggtaaa ggctccatca tgcgcctggg tgaagaccgt tccatggatg tggaaaccat      360

ctctaccggt tcgctttcac tggatatcgc gcttggggca ggtggtctgc cgatgggccg      420

tatcgtcgaa atctacggac cggaatcttc cggtaaaacc acgctgacgc tgcaggtgat      480

cgccgcagcg cagcgtgaag gtaaaacctg tgcgtttatc gatgctgaac acgcgctgga      540

cccaatctac gcacgtaaac tgggcgtcga tatcgacaac ctgctgtgct cccagccgga      600

caccggcgag caggcactgg aaatctgtga cgccctggcg cgttctggcg cagtagacgt      660

tatcgtcgtt gactccgtgg cggcactgac gccgaaagcg gaaatcgaag gcgaaatcgg      720

cgactctcac atgggccttg cggcacgtat gatgagccag gcgatgcgta agctggcggg      780

taacctgaag cagtccaaca cgctgctgat cttcatcaac cagatccgta tgaaaattgg      840

tgtgatgttc ggtaacccgg aaaccactac cggtggtaac gcgctgaaat tctacgcctc      900

tgttcgtctc gacatccgtc gtatcggcgc ggtgaaagag ggcgaaaacg tggtgggtag      960

cgaaacccgc gtgaaagtgg tgaagaacaa aatcgctgcg ccgtttaaac aggctgaatt     1020

ccagatcctc tacggcgaag gtatcaactt ctacggcgaa ctggttgacc tgggcgtaaa     1080

agagaagctg atcgagaaag caggcgcgtg gtacagctac aaaggtgaga agatcggtca     1140

gggtaaagcg aatgcgactg cctggctgaa agataacccg gaaaccgcga aagagatcga     1200

gaagaaagta cgtgagttgc tgctgagcaa cccgaactca acgccggatt tctctgtaga     1260

tgatagcgaa ggcgtagcag aaactaacga agatttttaa tcgtcttgtt tgatacacaa     1320

gggtcgcatc tgcggccctt ttgctttttt aagttgtaag gatatgccat gacagaatca     1380

acatcccgtc g                                                          1391


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  22
tgggtcacca gcaatcgcgc tg                                                22


<210>  23
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  23
catagctgtt tcctgtggaa ttgtgagcgc tcacaattcc acacaacata c                51


<210>  24
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  24
gctcacaatt ccacaggaaa cagctatgaa cacgattaac atcgctaaga ac               52


<210>  25
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  25
cagacatggc ctgcccggtt attattattt ttgacaccag accattacgc gaacgcgaag       60

tccgac                                                                  66


<210>  26
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  26
taatagggat aacagggtaa ttggtctggt gtcaaaaata ataataac                    48


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  27
aggatgagtg cacagccaga gc                                                22


<210>  28
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  28
accaattacc ctgttatccc tattagaaaa actcatcgag catcaaatg                   49


<210>  29
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  29
aataaccggg caggccatgt ctgcccgtag ggataacagg gtaatcagta atacaagggg       60

tgttatgag                                                               69


<210>  30
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  30
tgagctagct ctcactcgca atcaaattca gcc                                    33


<210>  31
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  31
tgacctaggt ggtgaacatg atgccgacaa tcg                                    33


<210>  32
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Binding site

<400>  32
gctcacaatt ccaca                                                        15


<210>  33
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  33
gaggcgtggt cttcgtggca taa                                               23


<210>  34
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  34
aaacaccgca tggaaaatca acaa                                              24


<210>  35
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  35
tctctgacca gacacccatc aac                                               23


<210>  36
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  36
tgattaatta atgctaagct gctggctgct gagg                                   34


<210>  37
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  37
tcctgtgtga aattgttacg cgaacgcgaa gtccgactct aag                         43


<210>  38
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  38
tcggacttcg cgttcgcgta acaatttcac acaggaaaca gctatgacc                   49


<210>  39
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  39
attattattt ttgacaccag accaactggt gaatggtagc gaccggcgct c                51


<210>  40
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  40
accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggcca tg               52


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  41
gtttgacggg tcttgctctg g                                                 21


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  42
tggcagaaga gggcgcatcc                                                   20


<210>  43
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  43
tctctgacca gacacccatc aac                                               23


<210>  44
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  44
acgcaaggaa gcagaacgga gaat                                              24


<210>  45
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  45
gctcgcaagt ctcgccgtat cag                                               23


<210>  46
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  46
tctgaacagt tccagtgcca gc                                                22


