                         SEQUENCE LISTING

<110>  UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
       BOSE, HIMANGSHU S.
 
<120>  REGULATION OF ALDOSTERONE SYNTHESIS FOR CONTROLLING HYPERTENSION

<130>  UF.618XC1 PCT

<150>  US 60/989,534
<151>  2007-11-21

<160>  5     

<170>  PatentIn version 3.3

<210>  1
<211>  524
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CYP11-Beta-2 with pause sequence insertions

<400>  1

Met Ala Leu Arg Val Thr Ala Asp Val Trp Leu Ala Arg Pro Trp Gln 
1               5                   10                  15      


Cys Leu His Arg Thr Arg Ala Leu Met Ala Leu Arg Val Thr Ala Asp 
            20                  25                  30          


Val Trp Leu Ala Arg Pro Trp Gln Cys Leu His Arg Thr Arg Ala Leu 
        35                  40                  45              


Gly Thr Thr Ala Thr Leu Ala Pro Lys Thr Leu Lys Pro Phe Glu Ala 
    50                  55                  60                  


Ile Pro Gln Tyr Ser Arg Asn Lys Trp Leu Lys Met Ile Gln Ile Leu 
65                  70                  75                  80  


Arg Glu Gln Gly Gln Glu Asn Leu His Leu Glu Met His Gln Ala Phe 
                85                  90                  95      


Gln Glu Leu Gly Pro Ile Phe Arg His Ser Ala Gly Gly Ala Gln Ile 
            100                 105                 110         


Val Ser Val Met Leu Pro Glu Asp Ala Glu Lys Leu His Gln Val Glu 
        115                 120                 125             


Ser Ile Leu Pro Arg Arg Met His Leu Glu Pro Trp Val Ala His Arg 
    130                 135                 140                 


Glu Leu Arg Gly Leu Arg Arg Gly Val Phe Leu Leu Asn Gly Ala Glu 
145                 150                 155                 160 


Trp Arg Phe Asn Arg Leu Lys Leu Asn Pro Asn Val Leu Ser Pro Lys 
                165                 170                 175     


Ala Val Gln Asn Phe Val Pro Met Val Asp Glu Val Ala Arg Asp Phe 
            180                 185                 190         


Leu Glu Ala Leu Lys Lys Lys Val Arg Gln Asn Ala Arg Gly Ser Leu 
        195                 200                 205             


Thr Met Asp Val Gln Gln Ser Leu Phe Asn Tyr Thr Ile Glu Ala Ser 
    210                 215                 220                 


Asn Phe Ala Leu Phe Gly Glu Arg Leu Gly Leu Leu Gly His Asp Leu 
225                 230                 235                 240 


Asn Pro Gly Ser Leu Lys Phe Ile His Ala Leu His Ser Met Phe Lys 
                245                 250                 255     


Ser Thr Thr Gln Leu Leu Phe Leu Pro Arg Ser Leu Thr Arg Trp Thr 
            260                 265                 270         


Ser Thr Gln Val Trp Lys Glu His Phe Asp Ala Trp Asp Val Ile Ser 
        275                 280                 285             


Glu Tyr Ala Asn Arg Cys Ile Trp Lys Val His Gln Glu Leu Arg Leu 
    290                 295                 300                 


Gly Ser Ser Gln Thr Tyr Ser Gly Ile Val Ala Ala Leu Ile Thr Gln 
305                 310                 315                 320 


Gly Ala Leu Pro Leu Asp Ala Ile Lys Ala Asn Ser Met Glu Leu Thr 
                325                 330                 335     


Ala Gly Ser Val Asp Thr Thr Ala Ile Pro Leu Val Met Thr Leu Phe 
            340                 345                 350         


Glu Leu Ala Arg Asn Pro Asp Val Gln Gln Ala Leu Arg Gln Glu Thr 
        355                 360                 365             


Leu Ala Ala Glu Ala Ser Ile Ala Ala Asn Pro Gln Lys Ala Met Ser 
    370                 375                 380                 


Asp Leu Pro Leu Leu Arg Ala Ala Leu Lys Glu Thr Leu Arg Leu Tyr 
385                 390                 395                 400 


Pro Val Gly Gly Phe Leu Glu Arg Ile Leu Asn Ser Asp Leu Val Leu 
                405                 410                 415     


Gln Asn Tyr His Val Pro Ala Gly Thr Leu Val Leu Leu Tyr Leu Tyr 
            420                 425                 430         


Ser Met Gly Arg Asn Pro Ala Val Phe Pro Arg Pro Glu Arg Tyr Met 
        435                 440                 445             


Pro Gln Arg Trp Leu Glu Arg Lys Arg Ser Phe Gln His Leu Ala Phe 
    450                 455                 460                 


Gly Phe Gly Val Arg Gln Cys Leu Gly Arg Arg Leu Ala Glu Val Glu 
465                 470                 475                 480 


Met Leu Leu Leu Leu His His Met Leu Lys Thr Phe Gln Val Glu Thr 
                485                 490                 495     


Leu Arg Gln Glu Asp Val Gln Met Ala Tyr Arg Phe Val Leu Met Pro 
            500                 505                 510         


Ser Ser Ser Pro Val Leu Thr Phe Arg Pro Ile Ser 
        515                 520                 


<210>  2
<211>  24
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pause sequence

<400>  2

Met Ala Leu Arg Val Thr Ala Asp Val Trp Leu Ala Arg Pro Trp Gln 
1               5                   10                  15      


Cys Leu His Arg Thr Arg Ala Leu 
            20                  


<210>  3
<211>  48
<212>  PRT
<213>  Artificial sequence

<220>
<223>  leader peptide

<400>  3

Met Ala Leu Arg Val Thr Ala Asp Val Trp Leu Ala Arg Pro Trp Gln 
1               5                   10                  15      


Cys Leu His Arg Thr Arg Ala Leu Met Ala Leu Arg Val Thr Ala Asp 
            20                  25                  30          


Val Trp Leu Ala Arg Pro Trp Gln Cys Leu His Arg Thr Arg Ala Leu 
        35                  40                  45              


<210>  4
<211>  482
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CYP11-Beta-2

<400>  4

Met Arg Thr Arg Ala Leu Gly Thr Thr Ala Thr Leu Ala Pro Lys Thr 
1               5                   10                  15      


Leu Lys Pro Phe Glu Ala Ile Pro Gln Tyr Ser Arg Asn Lys Trp Leu 
            20                  25                  30          


Lys Met Ile Gln Ile Leu Arg Glu Gln Gly Gln Glu Asn Leu His Leu 
        35                  40                  45              


Glu Met His Gln Ala Phe Gln Glu Leu Gly Pro Ile Phe Arg His Ser 
    50                  55                  60                  


Ala Gly Gly Ala Gln Ile Val Ser Val Met Leu Pro Glu Asp Ala Glu 
65                  70                  75                  80  


Lys Leu His Gln Val Glu Ser Ile Leu Pro Arg Arg Met His Leu Glu 
                85                  90                  95      


Pro Trp Val Ala His Arg Glu Leu Arg Gly Leu Arg Arg Gly Val Phe 
            100                 105                 110         


Leu Leu Asn Gly Ala Glu Trp Arg Phe Asn Arg Leu Lys Leu Asn Pro 
        115                 120                 125             


Asn Val Leu Ser Pro Lys Ala Val Gln Asn Phe Val Pro Met Val Asp 
    130                 135                 140                 


Glu Val Ala Arg Asp Phe Leu Glu Ala Leu Lys Lys Lys Val Arg Gln 
145                 150                 155                 160 


Asn Ala Arg Gly Ser Leu Thr Met Asp Val Gln Gln Ser Leu Phe Asn 
                165                 170                 175     


Tyr Thr Ile Glu Ala Ser Asn Phe Ala Leu Phe Gly Glu Arg Leu Gly 
            180                 185                 190         


Leu Leu Gly His Asp Leu Asn Pro Gly Ser Leu Lys Phe Ile His Ala 
        195                 200                 205             


Leu His Ser Met Phe Lys Ser Thr Thr Gln Leu Leu Phe Leu Pro Arg 
    210                 215                 220                 


Ser Leu Thr Arg Trp Thr Ser Thr Gln Val Trp Lys Glu His Phe Asp 
225                 230                 235                 240 


Ala Trp Asp Val Ile Ser Glu Tyr Ala Asn Arg Cys Ile Trp Lys Val 
                245                 250                 255     


His Gln Glu Leu Arg Leu Gly Ser Ser Gln Thr Tyr Ser Gly Ile Val 
            260                 265                 270         


Ala Ala Leu Ile Thr Gln Gly Ala Leu Pro Leu Asp Ala Ile Lys Ala 
        275                 280                 285             


Asn Ser Met Glu Leu Thr Ala Gly Ser Val Asp Thr Thr Ala Ile Pro 
    290                 295                 300                 


Leu Val Met Thr Leu Phe Glu Leu Ala Arg Asn Pro Asp Val Gln Gln 
305                 310                 315                 320 


Ala Leu Arg Gln Glu Thr Leu Ala Ala Glu Ala Ser Ile Ala Ala Asn 
                325                 330                 335     


Pro Gln Lys Ala Met Ser Asp Leu Pro Leu Leu Arg Ala Ala Leu Lys 
            340                 345                 350         


Glu Thr Leu Arg Leu Tyr Pro Val Gly Gly Phe Leu Glu Arg Ile Leu 
        355                 360                 365             


Asn Ser Asp Leu Val Leu Gln Asn Tyr His Val Pro Ala Gly Thr Leu 
    370                 375                 380                 


Val Leu Leu Tyr Leu Tyr Ser Met Gly Arg Asn Pro Ala Val Phe Pro 
385                 390                 395                 400 


Arg Pro Glu Arg Tyr Met Pro Gln Arg Trp Leu Glu Arg Lys Arg Ser 
                405                 410                 415     


Phe Gln His Leu Ala Phe Gly Phe Gly Val Arg Gln Cys Leu Gly Arg 
            420                 425                 430         


Arg Leu Ala Glu Val Glu Met Leu Leu Leu Leu His His Met Leu Lys 
        435                 440                 445             


Thr Phe Gln Val Glu Thr Leu Arg Gln Glu Asp Val Gln Met Ala Tyr 
    450                 455                 460                 


Arg Phe Val Leu Met Pro Ser Ser Ser Pro Val Leu Thr Phe Arg Pro 
465                 470                 475                 480 


Ile Ser 
        


<210>  5
<211>  477
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CYP11-Beta-2

<400>  5

Met Gly Thr Thr Ala Thr Leu Ala Pro Lys Thr Leu Lys Pro Phe Glu 
1               5                   10                  15      


Ala Ile Pro Gln Tyr Ser Arg Asn Lys Trp Leu Lys Met Ile Gln Ile 
            20                  25                  30          


Leu Arg Glu Gln Gly Gln Glu Asn Leu His Leu Glu Met His Gln Ala 
        35                  40                  45              


Phe Gln Glu Leu Gly Pro Ile Phe Arg His Ser Ala Gly Gly Ala Gln 
    50                  55                  60                  


Ile Val Ser Val Met Leu Pro Glu Asp Ala Glu Lys Leu His Gln Val 
65                  70                  75                  80  


Glu Ser Ile Leu Pro Arg Arg Met His Leu Glu Pro Trp Val Ala His 
                85                  90                  95      


Arg Glu Leu Arg Gly Leu Arg Arg Gly Val Phe Leu Leu Asn Gly Ala 
            100                 105                 110         


Glu Trp Arg Phe Asn Arg Leu Lys Leu Asn Pro Asn Val Leu Ser Pro 
        115                 120                 125             


Lys Ala Val Gln Asn Phe Val Pro Met Val Asp Glu Val Ala Arg Asp 
    130                 135                 140                 


Phe Leu Glu Ala Leu Lys Lys Lys Val Arg Gln Asn Ala Arg Gly Ser 
145                 150                 155                 160 


Leu Thr Met Asp Val Gln Gln Ser Leu Phe Asn Tyr Thr Ile Glu Ala 
                165                 170                 175     


Ser Asn Phe Ala Leu Phe Gly Glu Arg Leu Gly Leu Leu Gly His Asp 
            180                 185                 190         


Leu Asn Pro Gly Ser Leu Lys Phe Ile His Ala Leu His Ser Met Phe 
        195                 200                 205             


Lys Ser Thr Thr Gln Leu Leu Phe Leu Pro Arg Ser Leu Thr Arg Trp 
    210                 215                 220                 


Thr Ser Thr Gln Val Trp Lys Glu His Phe Asp Ala Trp Asp Val Ile 
225                 230                 235                 240 


Ser Glu Tyr Ala Asn Arg Cys Ile Trp Lys Val His Gln Glu Leu Arg 
                245                 250                 255     


Leu Gly Ser Ser Gln Thr Tyr Ser Gly Ile Val Ala Ala Leu Ile Thr 
            260                 265                 270         


Gln Gly Ala Leu Pro Leu Asp Ala Ile Lys Ala Asn Ser Met Glu Leu 
        275                 280                 285             


Thr Ala Gly Ser Val Asp Thr Thr Ala Ile Pro Leu Val Met Thr Leu 
    290                 295                 300                 


Phe Glu Leu Ala Arg Asn Pro Asp Val Gln Gln Ala Leu Arg Gln Glu 
305                 310                 315                 320 


Thr Leu Ala Ala Glu Ala Ser Ile Ala Ala Asn Pro Gln Lys Ala Met 
                325                 330                 335     


Ser Asp Leu Pro Leu Leu Arg Ala Ala Leu Lys Glu Thr Leu Arg Leu 
            340                 345                 350         


Tyr Pro Val Gly Gly Phe Leu Glu Arg Ile Leu Asn Ser Asp Leu Val 
        355                 360                 365             


Leu Gln Asn Tyr His Val Pro Ala Gly Thr Leu Val Leu Leu Tyr Leu 
    370                 375                 380                 


Tyr Ser Met Gly Arg Asn Pro Ala Val Phe Pro Arg Pro Glu Arg Tyr 
385                 390                 395                 400 


Met Pro Gln Arg Trp Leu Glu Arg Lys Arg Ser Phe Gln His Leu Ala 
                405                 410                 415     


Phe Gly Phe Gly Val Arg Gln Cys Leu Gly Arg Arg Leu Ala Glu Val 
            420                 425                 430         


Glu Met Leu Leu Leu Leu His His Met Leu Lys Thr Phe Gln Val Glu 
        435                 440                 445             


Thr Leu Arg Gln Glu Asp Val Gln Met Ala Tyr Arg Phe Val Leu Met 
    450                 455                 460                 


Pro Ser Ser Ser Pro Val Leu Thr Phe Arg Pro Ile Ser 
465                 470                 475         


