                         SEQUENCE LISTING

<110>  Cadila Healthcare Limited
       Mendiretta, Sanjeev Kumar
       Patel, Pankaj Ramanbhai
       Bandyopadhyay, Sanjay
       Saraswat, Vibhor
       Singh, Arun Kumar
 
<120>  FUSION PROTEIN SYSTEMS SUITABLE FOR HIGH EXPRESSION OF PEPTIDES

<130>  ZRC-BT-007

<150>  1848/MUM/2002
<151>  2007-09-21

<160>  19    

<170>  PatentIn version 3.3

<210>  1
<211>  528
<212>  DNA
<213>  Human Placental RNA

<400>  1
atgactccat taggtccagc aagctccctg ccccagagct tcctgctcaa gtgcttagag     60

caagtgagga agatccaggg cgatggcgca gcgctccagg agaagctgtg tgccacctac    120

aagctgtgcc accccgagga gctggtgctg ctcggacact ctctgggcat cccctgggct    180

cccctgagca gctgccccag ccaggccctg cagctggcag gctgcttgag ccaactccat    240

agcggccttt tcctctacca ggggctcctg caggccctgg aagggatctc ccccgagttg    300

ggtcccacct tggacacact gcagctggac gtcgccgact ttgccaccac catctggcag    360

cagatggaag aactggggat ggcccctgcc ctgcagccca cccagggtgc catgccggcc    420

ttcgcctctg ctttccagcg ccgggcagga ggggtcctag ttgcctccca tctgcagagc    480

ttcctggagg tgtcgtaccg cgttctacgc caccttgccc agccctga                 528


<210>  2
<211>  175
<212>  PRT
<213>  Human Placental RNA

<400>  2

Met Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu 
            20                  25                  30          


Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
    50                  55                  60                  


Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His 
65                  70                  75                  80  


Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
            100                 105                 110         


Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala 
        115                 120                 125             


Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro 
                165                 170                 175 


<210>  3
<211>  23
<212>  DNA
<213>  Synthetic Oligonucleotide

<400>  3
gcgcatatga ctccattagg tcc                                             23


<210>  4
<211>  32
<212>  DNA
<213>  Synthetic Oligonucleotide

<400>  4
gcggatcctc agggctgggc aaggtggcgt ag                                   32


<210>  5
<211>  29
<212>  DNA
<213>  Synthetic Oligonucleotide

<400>  5
gcggatccgg gctgggcaag gtggcgtag                                       29


<210>  6
<211>  651
<212>  PRT
<213>  Human Placental RNA

<400>  6

Ala Thr Gly Ala Cys Thr Cys Cys Ala Thr Thr Ala Gly Gly Thr Cys 
1               5                   10                  15      


Cys Ala Gly Cys Ala Ala Gly Cys Thr Cys Cys Cys Thr Gly Cys Cys 
            20                  25                  30          


Cys Cys Ala Gly Ala Gly Cys Thr Thr Cys Cys Thr Gly Cys Thr Cys 
        35                  40                  45              


Ala Ala Gly Thr Gly Cys Thr Thr Ala Gly Ala Gly Cys Ala Ala Gly 
    50                  55                  60                  


Thr Gly Ala Gly Gly Ala Ala Gly Ala Thr Cys Cys Ala Gly Gly Gly 
65                  70                  75                  80  


Cys Gly Ala Thr Gly Gly Cys Gly Cys Ala Gly Cys Gly Cys Thr Cys 
                85                  90                  95      


Cys Ala Gly Gly Ala Gly Ala Ala Gly Cys Thr Gly Thr Gly Thr Gly 
            100                 105                 110         


Cys Cys Ala Cys Cys Thr Ala Cys Ala Ala Gly Cys Thr Gly Thr Gly 
        115                 120                 125             


Cys Cys Ala Cys Cys Cys Cys Gly Ala Gly Gly Ala Gly Cys Thr Gly 
    130                 135                 140                 


Gly Thr Gly Cys Thr Gly Cys Thr Cys Gly Gly Ala Cys Ala Cys Thr 
145                 150                 155                 160 


Cys Thr Cys Thr Gly Gly Gly Cys Ala Thr Cys Cys Cys Cys Thr Gly 
                165                 170                 175     


Gly Gly Cys Thr Cys Cys Cys Cys Thr Gly Ala Gly Cys Ala Gly Cys 
            180                 185                 190         


Thr Gly Cys Cys Cys Cys Ala Gly Cys Cys Ala Gly Gly Cys Cys Cys 
        195                 200                 205             


Thr Gly Cys Ala Gly Cys Thr Gly Gly Cys Ala Gly Gly Cys Thr Gly 
    210                 215                 220                 


Cys Thr Thr Gly Ala Gly Cys Cys Ala Ala Cys Thr Cys Cys Ala Thr 
225                 230                 235                 240 


Ala Gly Cys Gly Gly Cys Cys Thr Thr Thr Thr Cys Cys Thr Cys Thr 
                245                 250                 255     


Ala Cys Cys Ala Gly Gly Gly Gly Cys Thr Cys Cys Thr Gly Cys Ala 
            260                 265                 270         


Gly Gly Cys Cys Cys Thr Gly Gly Ala Ala Gly Gly Gly Ala Thr Cys 
        275                 280                 285             


Thr Cys Cys Cys Cys Cys Gly Ala Gly Thr Thr Gly Gly Gly Thr Cys 
    290                 295                 300                 


Cys Cys Ala Cys Cys Thr Thr Gly Gly Ala Cys Ala Cys Ala Cys Thr 
305                 310                 315                 320 


Gly Cys Ala Gly Cys Thr Gly Gly Ala Cys Gly Thr Cys Gly Cys Cys 
                325                 330                 335     


Gly Ala Cys Thr Thr Thr Gly Cys Cys Ala Cys Cys Ala Cys Cys Ala 
            340                 345                 350         


Thr Cys Thr Gly Gly Cys Ala Gly Cys Ala Gly Ala Thr Gly Gly Ala 
        355                 360                 365             


Ala Gly Ala Ala Cys Thr Gly Gly Gly Gly Ala Thr Gly Gly Cys Cys 
    370                 375                 380                 


Cys Cys Thr Gly Cys Cys Cys Thr Gly Cys Ala Gly Cys Cys Cys Ala 
385                 390                 395                 400 


Cys Cys Cys Ala Gly Gly Gly Thr Gly Cys Cys Ala Thr Gly Cys Cys 
                405                 410                 415     


Gly Gly Cys Cys Thr Thr Cys Gly Cys Cys Thr Cys Thr Gly Cys Thr 
            420                 425                 430         


Thr Thr Cys Cys Ala Gly Cys Gly Cys Cys Gly Gly Gly Cys Ala Gly 
        435                 440                 445             


Gly Ala Gly Gly Gly Gly Thr Cys Cys Thr Ala Gly Thr Thr Gly Cys 
    450                 455                 460                 


Cys Thr Cys Cys Cys Ala Thr Cys Thr Gly Cys Ala Gly Ala Gly Cys 
465                 470                 475                 480 


Thr Thr Cys Cys Thr Gly Gly Ala Gly Gly Thr Gly Thr Cys Gly Thr 
                485                 490                 495     


Ala Cys Cys Gly Cys Gly Thr Thr Cys Thr Ala Cys Gly Cys Cys Ala 
            500                 505                 510         


Cys Cys Thr Thr Gly Cys Cys Cys Ala Gly Cys Cys Cys Gly Gly Ala 
        515                 520                 525             


Thr Cys Cys Gly Ala Thr Gly Ala Thr Gly Ala Thr Gly Ala Thr Ala 
    530                 535                 540                 


Ala Ala Thr Cys Gly Gly Thr Gly Thr Cys Gly Gly Ala Ala Ala Thr 
545                 550                 555                 560 


Ala Cys Ala Gly Cys Thr Thr Ala Thr Gly Cys Ala Thr Ala Ala Cys 
                565                 570                 575     


Cys Thr Gly Gly Gly Ala Ala Ala Ala Cys Ala Thr Cys Thr Gly Ala 
            580                 585                 590         


Ala Cys Thr Cys Gly Ala Thr Gly Gly Ala Gly Ala Gly Ala Gly Thr 
        595                 600                 605             


Ala Gly Ala Ala Thr Gly Gly Cys Thr Gly Cys Gly Thr Ala Ala Gly 
    610                 615                 620                 


Ala Ala Gly Cys Thr Gly Cys Ala Gly Gly Ala Thr Gly Thr Gly Cys 
625                 630                 635                 640 


Ala Cys Ala Ala Thr Thr Thr Thr Thr Ala Gly 
                645                 650     


<210>  7
<211>  216
<212>  PRT
<213>  Human Placental RNA

<400>  7

Met Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu 
            20                  25                  30          


Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
    50                  55                  60                  


Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His 
65                  70                  75                  80  


Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
            100                 105                 110         


Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala 
        115                 120                 125             


Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Gly 
                165                 170                 175     


Ser Asp Asp Asp Asp Lys Ser Val Ser Glu Ile Gln Leu Met His Asn 
            180                 185                 190         


Leu Gly Lys His Leu Asn Ser Met Glu Arg Val Glu Trp Leu Arg Lys 
        195                 200                 205             


Lys Leu Gln Asp Val His Asn Phe 
    210                 215     


<210>  8
<211>  104
<212>  DNA
<213>  Synthetic Oligonucleotide

<400>  8
cggtgtcgga aatacagctt atgcataacc tgggaaaaca tctgaactcg atggagagag     60

tagaatggct gcgtaagaag ctgcaggatg tgcacaattt ttag                     104


<210>  9
<211>  104
<212>  DNA
<213>  Synthetic Oligonucleotide

<400>  9
ctaaaaattg tgcacatcct gcagcttctt acgcagccat tctactctct ccatcgagtt     60

cagatgtttt cccaggttat gcataagctg tatttccgac accg                     104


<210>  10
<211>  42
<212>  DNA
<213>  Synthetic Oligonucleotide

<400>  10
gcggatccga tgatgatgat aaatcggtgt cggaaataca gc                        42


<210>  11
<211>  34
<212>  DNA
<213>  Synthetic Oligonucleotide

<400>  11
ttattgctca gctcaaaaat tgtgcacatc ttgg                                 34


<210>  12
<211>  795
<212>  DNA
<213>  Human Placental RNA

<400>  12
atgactccat taggtccagc aagctccctg ccccagagct tcctgctcaa gtgcttagag     60

caagtgagga agatccaggg cgatggcgca gcgctccagg agaagctgtg tgccacctac    120

aagctgtgcc accccgagga gctggtgctg ctcggacact ctctgggcat cccctgggct    180

cccctgagca gctgccccag ccaggccctg cagctggcag gctgcttgag ccaactccat    240

agcggccttt tcctctacca ggggctcctg caggccctgg aagggatctc ccccgagttg    300

ggtcccacct tggacacact gcagctggac gtcgccgact ttgccaccac catctggcag    360

cagatggaag aactggggat ggcccctgcc ctgcagccca cccagggtgc catgccggcc    420

ttcgcctctg ctttccagcg ccgggcagga ggggtcctag ttgcctccca tctgcagagc    480

ttcctggagg tgtcgtaccg cgttctacgc caccttgccc agcccggatc catgtttgtg    540

aaccaacacc tgtgcggctc acacctggtg gaagctctct acctagtgtg cggggaacga    600

ggcttcttct acacacccaa gacccgccgg gaggcagagg acctgcaggt ggggcaggtg    660

gagctgggcg ggggccctgg tgcaggcagc ctgcagccct tggccctgga ggggtccctg    720

cagaagcgtg gcattgtgga acaatgctgt accagcatct gctccctcta ccagctggag    780

aactactgca actag                                                     795


<210>  13
<211>  264
<212>  PRT
<213>  Human Placental RNA

<400>  13

Met Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu 
            20                  25                  30          


Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
    50                  55                  60                  


Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His 
65                  70                  75                  80  


Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
            100                 105                 110         


Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala 
        115                 120                 125             


Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Gly 
                165                 170                 175     


Ser Met Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala 
            180                 185                 190         


Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
        195                 200                 205             


Arg Arg Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly 
    210                 215                 220                 


Gly Pro Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu 
225                 230                 235                 240 


Gln Lys Arg Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu 
                245                 250                 255     


Tyr Gln Leu Glu Asn Tyr Cys Asn 
            260                 


<210>  14
<211>  32
<212>  DNA
<213>  Synthetic Oligonucleotide

<400>  14
gcggatccat gtttgtgaac cagcatctgt gc                                   32


<210>  15
<211>  36
<212>  DNA
<213>  Synthetic Oligonucleotide

<400>  15
ttattgctca gcttagttgc aatagttttc cagctg                               36


<210>  16
<211>  579
<212>  DNA
<213>  Human Placental RNA

<400>  16
atgactccat taggtccagc aagctccctg ccccagagct tcctgctcaa gtgcttagag     60

caagtgagga agatccaggg cgatggcgca gcgctccagg agaagctgtg tgccacctac    120

aagctgtgcc accccgagga gctggtgctg ctcggacact ctctgggcat cccctgggct    180

cccctgagca gctgccccag ccaggccctg cagctggcag gctgcttgag ccaactccat    240

agcggccttt tcctctacca ggggctcctg caggccctgg aagggatctc ccccgagttg    300

ggtcccacct tggacacact gcagctggac gtcgccgact ttgccaccac catctggcag    360

cagatggaag aactggggat ggcccctgcc ctgcagccca cccagggtgc catgccggcc    420

ttcgcctctg ctttccagcg ccgggcagga ggggtcctag ttgcctccca tctgcagagc    480

ttcctggagg tgtcgtaccg cgttctacgc caccttgccc agcccggatc cgatgatgat    540

gataaagatc gcgtgtatat tcatccgttt catctgtag                           579


<210>  17
<211>  192
<212>  PRT
<213>  Human Placental RNA

<400>  17

Met Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu 
            20                  25                  30          


Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
    50                  55                  60                  


Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His 
65                  70                  75                  80  


Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
            100                 105                 110         


Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala 
        115                 120                 125             


Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro Gly 
                165                 170                 175     


Ser Asp Asp Asp Asp Lys Asp Arg Val Tyr Ile His Pro Phe His Leu 
            180                 185                 190         


<210>  18
<211>  38
<212>  DNA
<213>  Synthetic Oligonucleotide

<400>  18
cccctctaga aataattttg tttaacttta agaaggag                             38


<210>  19
<211>  66
<212>  DNA
<213>  Synthetic Oligonucleotide

<400>  19
cgccgagtcg tcacagatga aacggatgaa tatacacgcg atctttatca tcatcatcgg     60

atccgc                                                                66


