                               SEQUENCE LISTING

<110> DANISCO US INC., GENENCOR DIVISION 
 
<120> VARIANTS OF BACILLUS SP. TS-23 ALPHA-AMYLASE WITH ALTERED 
      PROPERTIES

<130> 48452-0031-00-WO

<140>
<141>

<150> 61/059,403
<151> 2008-06-06

<150> 61/026,056
<151> 2008-02-04

<150> 60/985,619
<151> 2007-11-05

<160> 18    

<170> PatentIn version 3.5

<210> 1
<211> 583
<212> PRT
<213> Bacillus sp.

<400> 1
Asn Thr Ala Pro Ile Asn Glu Thr Met Met Gln Tyr Phe Glu Trp Asp 
1               5                   10                  15      


Leu Pro Asn Asp Gly Thr Leu Trp Thr Lys Val Lys Asn Glu Ala Ala 
            20                  25                  30          


Asn Leu Ser Ser Leu Gly Ile Thr Ala Leu Trp Leu Pro Pro Ala Tyr 
        35                  40                  45              


Lys Gly Thr Ser Gln Ser Asp Val Gly Tyr Gly Val Tyr Asp Leu Tyr 
    50                  55                  60                  


Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Ile Arg Thr Lys Tyr Gly 
65                  70                  75                  80  


Thr Lys Thr Gln Tyr Ile Gln Ala Ile Gln Ala Ala Lys Ala Ala Gly 
                85                  90                  95      


Met Gln Val Tyr Ala Asp Val Val Phe Asn His Lys Ala Gly Ala Asp 
            100                 105                 110         


Gly Thr Glu Phe Val Asp Ala Val Glu Val Asp Pro Ser Asn Arg Asn 
        115                 120                 125             


Gln Glu Thr Ser Gly Thr Tyr Gln Ile Gln Ala Trp Thr Lys Phe Asp 
    130                 135                 140                 


Phe Pro Gly Arg Gly Asn Thr Tyr Ser Ser Phe Lys Trp Arg Trp Tyr 
145                 150                 155                 160 


His Phe Asp Gly Thr Asp Trp Asp Glu Ser Arg Lys Leu Asn Arg Ile 
                165                 170                 175     


Tyr Lys Phe Arg Ser Thr Gly Lys Ala Trp Asp Trp Glu Val Asp Thr 
            180                 185                 190         


Glu Asn Gly Asn Tyr Asp Tyr Leu Met Phe Ala Asp Leu Asp Met Asp 
        195                 200                 205             


His Pro Glu Val Val Thr Glu Leu Lys Asn Trp Gly Thr Trp Tyr Val 
    210                 215                 220                 


Asn Thr Thr Asn Ile Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile 
225                 230                 235                 240 


Lys Tyr Ser Phe Phe Pro Asp Trp Leu Thr Tyr Val Arg Asn Gln Thr 
                245                 250                 255     


Gly Lys Asn Leu Phe Ala Val Gly Glu Phe Trp Ser Tyr Asp Val Asn 
            260                 265                 270         


Lys Leu His Asn Tyr Ile Thr Lys Thr Asn Gly Ser Met Ser Leu Phe 
        275                 280                 285             


Asp Ala Pro Leu His Asn Asn Phe Tyr Thr Ala Ser Lys Ser Ser Gly 
    290                 295                 300                 


Tyr Phe Asp Met Arg Tyr Leu Leu Asn Asn Thr Leu Met Lys Asp Gln 
305                 310                 315                 320 


Pro Ser Leu Ala Val Thr Leu Val Asp Asn His Asp Thr Gln Pro Gly 
                325                 330                 335     


Gln Ser Leu Gln Ser Trp Val Glu Pro Trp Phe Lys Pro Leu Ala Tyr 
            340                 345                 350         


Ala Phe Ile Leu Thr Arg Gln Glu Gly Tyr Pro Cys Val Phe Tyr Gly 
        355                 360                 365             


Asp Tyr Tyr Gly Ile Pro Lys Tyr Asn Ile Pro Gly Leu Lys Ser Lys 
    370                 375                 380                 


Ile Asp Pro Leu Leu Ile Ala Arg Arg Asp Tyr Ala Tyr Gly Thr Gln 
385                 390                 395                 400 


Arg Asp Tyr Ile Asp His Gln Asp Ile Ile Gly Trp Thr Arg Glu Gly 
                405                 410                 415     


Ile Asp Thr Lys Pro Asn Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly 
            420                 425                 430         


Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Lys Lys His Ala Gly Lys 
        435                 440                 445             


Val Phe Tyr Asp Leu Thr Gly Asn Arg Ser Asp Thr Val Thr Ile Asn 
    450                 455                 460                 


Ala Asp Gly Trp Gly Glu Phe Lys Val Asn Gly Gly Ser Val Ser Ile 
465                 470                 475                 480 


Trp Val Ala Lys Thr Ser Asn Val Thr Phe Thr Val Asn Asn Ala Thr 
                485                 490                 495     


Thr Thr Ser Gly Gln Asn Val Tyr Val Val Ala Asn Ile Pro Glu Leu 
            500                 505                 510         


Gly Asn Trp Asn Thr Ala Asn Ala Ile Lys Met Asn Pro Ser Ser Tyr 
        515                 520                 525             


Pro Thr Trp Lys Ala Thr Ile Ala Leu Pro Gln Gly Lys Ala Ile Glu 
    530                 535                 540                 


Phe Lys Phe Ile Lys Lys Asp Gln Ala Gly Asn Val Ile Trp Glu Ser 
545                 550                 555                 560 


Thr Ser Asn Arg Thr Tyr Thr Val Pro Phe Ser Ser Thr Gly Ser Tyr 
                565                 570                 575     


Thr Ala Ser Trp Asn Val Pro 
            580             


<210> 2
<211> 484
<212> PRT
<213> Bacillus sp.

<400> 2
Asn Thr Ala Pro Ile Asn Glu Thr Met Met Gln Tyr Phe Glu Trp Asp 
1               5                   10                  15      


Leu Pro Asn Asp Gly Thr Leu Trp Thr Lys Val Lys Asn Glu Ala Ala 
            20                  25                  30          


Asn Leu Ser Ser Leu Gly Ile Thr Ala Leu Trp Leu Pro Pro Ala Tyr 
        35                  40                  45              


Lys Gly Thr Ser Gln Ser Asp Val Gly Tyr Gly Val Tyr Asp Leu Tyr 
    50                  55                  60                  


Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Ile Arg Thr Lys Tyr Gly 
65                  70                  75                  80  


Thr Lys Thr Gln Tyr Ile Gln Ala Ile Gln Ala Ala Lys Ala Ala Gly 
                85                  90                  95      


Met Gln Val Tyr Ala Asp Val Val Phe Asn His Lys Ala Gly Ala Asp 
            100                 105                 110         


Gly Thr Glu Phe Val Asp Ala Val Glu Val Asp Pro Ser Asn Arg Asn 
        115                 120                 125             


Gln Glu Thr Ser Gly Thr Tyr Gln Ile Gln Ala Trp Thr Lys Phe Asp 
    130                 135                 140                 


Phe Pro Gly Arg Gly Asn Thr Tyr Ser Ser Phe Lys Trp Arg Trp Tyr 
145                 150                 155                 160 


His Phe Asp Gly Thr Asp Trp Asp Glu Ser Arg Lys Leu Asn Arg Ile 
                165                 170                 175     


Tyr Lys Phe Arg Ser Thr Gly Lys Ala Trp Asp Trp Glu Val Asp Thr 
            180                 185                 190         


Glu Asn Gly Asn Tyr Asp Tyr Leu Met Phe Ala Asp Leu Asp Met Asp 
        195                 200                 205             


His Pro Glu Val Val Thr Glu Leu Lys Asn Trp Gly Thr Trp Tyr Val 
    210                 215                 220                 


Asn Thr Thr Asn Ile Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile 
225                 230                 235                 240 


Lys Tyr Ser Phe Phe Pro Asp Trp Leu Thr Tyr Val Arg Asn Gln Thr 
                245                 250                 255     


Gly Lys Asn Leu Phe Ala Val Gly Glu Phe Trp Ser Tyr Asp Val Asn 
            260                 265                 270         


Lys Leu His Asn Tyr Ile Thr Lys Thr Asn Gly Ser Met Ser Leu Phe 
        275                 280                 285             


Asp Ala Pro Leu His Asn Asn Phe Tyr Thr Ala Ser Lys Ser Ser Gly 
    290                 295                 300                 


Tyr Phe Asp Met Arg Tyr Leu Leu Asn Asn Thr Leu Met Lys Asp Gln 
305                 310                 315                 320 


Pro Ser Leu Ala Val Thr Leu Val Asp Asn His Asp Thr Gln Pro Gly 
                325                 330                 335     


Gln Ser Leu Gln Ser Trp Val Glu Pro Trp Phe Lys Pro Leu Ala Tyr 
            340                 345                 350         


Ala Phe Ile Leu Thr Arg Gln Glu Gly Tyr Pro Cys Val Phe Tyr Gly 
        355                 360                 365             


Asp Tyr Tyr Gly Ile Pro Lys Tyr Asn Ile Pro Gly Leu Lys Ser Lys 
    370                 375                 380                 


Ile Asp Pro Leu Leu Ile Ala Arg Arg Asp Tyr Ala Tyr Gly Thr Gln 
385                 390                 395                 400 


Arg Asp Tyr Ile Asp His Gln Asp Ile Ile Gly Trp Thr Arg Glu Gly 
                405                 410                 415     


Ile Asp Thr Lys Pro Asn Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly 
            420                 425                 430         


Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Lys Lys His Ala Gly Lys 
        435                 440                 445             


Val Phe Tyr Asp Leu Thr Gly Asn Arg Ser Asp Thr Val Thr Ile Asn 
    450                 455                 460                 


Ala Asp Gly Trp Gly Glu Phe Lys Val Asn Gly Gly Ser Val Ser Ile 
465                 470                 475                 480 


Trp Val Ala Lys 
                


<210> 3
<211> 1752
<212> DNA
<213> Bacillus sp.

<400> 3
aatacggcgc cgatcaacga aacgatgatg cagtattttg aatgggatct gccgaatgat     60

ggaacgctgt ggacgaaagt caaaaacgaa gcggcgaatc ttagcagcct gggaatcaca    120

gcactttggc ttccgccggc atataaagga acgagccaaa gcgatgtcgg ctatggcgtc    180

tatgatctgt atgacctggg cgaatttaac caaaaaggca cgatccggac gaaatatggc    240

acgaaaacac agtatatcca agcgatccag gcagcaaaag cagcaggcat gcaagtctat    300

gccgacgtcg tctttaatca taaagcggga gcggatggca cagaatttgt cgatgccgtc    360

gaagttgatc cgagcaacag aaaccaagaa acgagcggca cgtatcaaat ccaagcgtgg    420

acgaaatttg attttccggg cagaggcaat acgtatagca gctttaaatg gcgctggtat    480

cattttgacg gcacggattg ggatgaaagc agaaaactga accggatcta taaatttcgg    540

agcacgggca aagcatggga ttgggaagtc gatacggaaa acggcaacta tgactatctg    600

atgtttgccg atctggatat ggatcatccg gaagtcgtca cggaactgaa aaattggggc    660

acgtggtatg ttaatacgac gaacatcgat ggctttagac tggatgccgt caaacatatc    720

aaatatagct tttttccgga ctggctgacg tatgtcagaa accagacggg caaaaacctt    780

tttgccgtcg gcgaattttg gagctatgac gtcaacaaac ttcataacta tatcacgaaa    840

acgaacggca gcatgagcct ttttgatgcc ccgcttcata acaactttta tacggcgagc    900

aaaagctcag gctattttga tatgagatat ctgctgaaca acacgctgat gaaagatcaa    960

ccgagcctgg cagtcacact ggtcgataac catgatacac aaccgggcca aagccttcaa   1020

agctgggtcg aaccgtggtt taaaccgctg gcgtatgcct ttatcctgac gagacaagaa   1080

gggtatcctt gcgtctttta tggcgactat tatggcatcc cgaaatataa tatcccgggc   1140

ctgaaaagca aaatcgatcc gctgctgatc gccagacggg attatgccta tggcacacag   1200

cgggattata tcgaccatca ggacatcatc ggctggacaa gagaaggcat cgatacgaaa   1260

ccgaatagcg gactggcagc actgattaca gatggaccgg gcggaagcaa atggatgtat   1320

gtcggcaaaa aacatgccgg caaagtcttt tatgatctga cgggcaacag aagcgatacg   1380

gtcacgatca atgctgatgg ctggggagaa tttaaagtca atggcggcag cgtttcaatc   1440

tgggtcgcca aaacgagcaa tgtcacgttt acggtcaaca atgccacgac aacgagcggc   1500

caaaatgtct atgtcgtcgc caatatcccg gaactgggca attggaatac ggcgaacgca   1560

atcaaaatga acccgagcag ctatccgaca tggaaagcga caatcgctct gccgcaagga   1620

aaagcgatcg aatttaaatt tatcaaaaaa gaccaggcgg gcaatgttat ttgggaaagc   1680

acgagcaata gaacgtatac ggtcccgttt agcagcacag gaagctatac agcgagctgg   1740

aatgttccgt ga                                                       1752


<210> 4
<211> 1455
<212> DNA
<213> Bacillus sp.

<400> 4
aatacggcgc cgatcaacga aacgatgatg cagtattttg aatgggatct gccgaatgat     60

ggaacgctgt ggacgaaagt caaaaacgaa gcggcgaatc ttagcagcct gggaatcaca    120

gcactttggc ttccgccggc atataaagga acgagccaaa gcgatgtcgg ctatggcgtc    180

tatgatctgt atgacctggg cgaatttaac caaaaaggca cgatccggac gaaatatggc    240

acgaaaacac agtatatcca agcgatccag gcagcaaaag cagcaggcat gcaagtctat    300

gccgacgtcg tctttaatca taaagcggga gcggatggca cagaatttgt cgatgccgtc    360

gaagttgatc cgagcaacag aaaccaagaa acgagcggca cgtatcaaat ccaagcgtgg    420

acgaaatttg attttccggg cagaggcaat acgtatagca gctttaaatg gcgctggtat    480

cattttgacg gcacggattg ggatgaaagc agaaaactga accggatcta taaatttcgg    540

agcacgggca aagcatggga ttgggaagtc gatacggaaa acggcaacta tgactatctg    600

atgtttgccg atctggatat ggatcatccg gaagtcgtca cggaactgaa aaattggggc    660

acgtggtatg ttaatacgac gaacatcgat ggctttagac tggatgccgt caaacatatc    720

aaatatagct tttttccgga ctggctgacg tatgtcagaa accagacggg caaaaacctt    780

tttgccgtcg gcgaattttg gagctatgac gtcaacaaac ttcataacta tatcacgaaa    840

acgaacggca gcatgagcct ttttgatgcc ccgcttcata acaactttta tacggcgagc    900

aaaagctcag gctattttga tatgagatat ctgctgaaca acacgctgat gaaagatcaa    960

ccgagcctgg cagtcacact ggtcgataac catgatacac aaccgggcca aagccttcaa   1020

agctgggtcg aaccgtggtt taaaccgctg gcgtatgcct ttatcctgac gagacaagaa   1080

gggtatcctt gcgtctttta tggcgactat tatggcatcc cgaaatataa tatcccgggc   1140

ctgaaaagca aaatcgatcc gctgctgatc gccagacggg attatgccta tggcacacag   1200

cgggattata tcgaccatca ggacatcatc ggctggacaa gagaaggcat cgatacgaaa   1260

ccgaatagcg gactggcagc actgattaca gatggaccgg gcggaagcaa atggatgtat   1320

gtcggcaaaa aacatgccgg caaagtcttt tatgatctga cgggcaacag aagcgatacg   1380

gtcacgatca atgctgatgg ctggggagaa tttaaagtca atggcggcag cgtttcaatc   1440

tgggtcgcca aatga                                                    1455


<210> 5
<211> 482
<212> PRT
<213> Bacillus sp.

<400> 5
Asn Thr Ala Pro Ile Asn Glu Thr Met Met Gln Tyr Phe Glu Trp Asp 
1               5                   10                  15      


Leu Pro Asn Asp Gly Thr Leu Trp Thr Lys Val Lys Asn Glu Ala Ala 
            20                  25                  30          


Asn Leu Ser Ser Leu Gly Ile Thr Ala Leu Trp Leu Pro Pro Ala Tyr 
        35                  40                  45              


Lys Gly Thr Ser Gln Ser Asp Val Gly Tyr Gly Val Tyr Asp Leu Tyr 
    50                  55                  60                  


Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Ile Arg Thr Lys Tyr Gly 
65                  70                  75                  80  


Thr Lys Thr Gln Tyr Ile Gln Ala Ile Gln Ala Ala Lys Ala Ala Gly 
                85                  90                  95      


Met Gln Val Tyr Ala Asp Val Val Phe Asn His Lys Ala Gly Ala Asp 
            100                 105                 110         


Gly Thr Glu Phe Val Asp Ala Val Glu Val Asp Pro Ser Asn Arg Asn 
        115                 120                 125             


Gln Glu Thr Ser Gly Thr Tyr Gln Ile Gln Ala Trp Thr Lys Phe Asp 
    130                 135                 140                 


Phe Pro Gly Arg Gly Asn Thr Tyr Ser Ser Phe Lys Trp Arg Trp Tyr 
145                 150                 155                 160 


His Phe Asp Gly Thr Asp Trp Asp Glu Ser Arg Lys Leu Asn Arg Ile 
                165                 170                 175     


Tyr Lys Phe Thr Gly Lys Ala Trp Asp Trp Glu Val Asp Thr Glu Asn 
            180                 185                 190         


Gly Asn Tyr Asp Tyr Leu Met Phe Ala Asp Leu Asp Met Asp His Pro 
        195                 200                 205             


Glu Val Val Thr Glu Leu Lys Asn Trp Gly Thr Trp Tyr Val Asn Thr 
    210                 215                 220                 


Thr Asn Ile Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys Tyr 
225                 230                 235                 240 


Ser Phe Phe Pro Asp Trp Leu Thr Tyr Val Arg Asn Gln Thr Gly Lys 
                245                 250                 255     


Asn Leu Phe Ala Val Gly Glu Phe Trp Ser Tyr Asp Val Asn Lys Leu 
            260                 265                 270         


His Asn Tyr Ile Thr Lys Thr Asn Gly Ser Met Ser Leu Phe Asp Ala 
        275                 280                 285             


Pro Leu His Asn Asn Phe Tyr Thr Ala Ser Lys Ser Ser Gly Tyr Phe 
    290                 295                 300                 


Asp Met Arg Tyr Leu Leu Asn Asn Thr Leu Met Lys Asp Gln Pro Ser 
305                 310                 315                 320 


Leu Ala Val Thr Leu Val Asp Asn His Asp Thr Gln Pro Gly Gln Ser 
                325                 330                 335     


Leu Gln Ser Trp Val Glu Pro Trp Phe Lys Pro Leu Ala Tyr Ala Phe 
            340                 345                 350         


Ile Leu Thr Arg Gln Glu Gly Tyr Pro Cys Val Phe Tyr Gly Asp Tyr 
        355                 360                 365             


Tyr Gly Ile Pro Lys Tyr Asn Ile Pro Gly Leu Lys Ser Lys Ile Asp 
    370                 375                 380                 


Pro Leu Leu Ile Ala Arg Arg Asp Tyr Ala Tyr Gly Thr Gln Arg Asp 
385                 390                 395                 400 


Tyr Ile Asp His Gln Asp Ile Ile Gly Trp Thr Arg Glu Gly Ile Asp 
                405                 410                 415     


Thr Lys Pro Asn Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro Gly 
            420                 425                 430         


Gly Ser Lys Trp Met Tyr Val Gly Lys Lys His Ala Gly Lys Val Phe 
        435                 440                 445             


Tyr Asp Leu Thr Gly Asn Arg Ser Asp Thr Val Thr Ile Asn Ala Asp 
    450                 455                 460                 


Gly Trp Gly Glu Phe Lys Val Asn Gly Gly Ser Val Ser Ile Trp Val 
465                 470                 475                 480 


Ala Lys 
        


<210> 6
<211> 1977
<212> DNA
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      polynucleotide"

<400> 6
aaaattcgga atatttatac aatatcatat gtttcacatt gaaaggggag gagaatcatg     60

aaacaacaaa aacggcttta cgcccgattg ctgacgctgt tatttgcgct catcttcttg    120

ctgcctcatt ctgcagcttc agcaaatacg gcgccgatca acgaaacgat gatgcagtat    180

tttgaatggg atctgccgaa tgatggaacg ctgtggacga aagtcaaaaa cgaagcggcg    240

aatcttagca gcctgggaat cacagcactt tggcttccgc cggcatataa aggaacgagc    300

caaagcgatg tcggctatgg cgtctatgat ctgtatgacc tgggcgaatt taaccaaaaa    360

ggcacgatcc ggacgaaata tggcacgaaa acacagtata tccaagcgat ccaggcagca    420

aaagcagcag gcatgcaagt ctatgccgac gtcgtcttta atcataaagc gggagcggat    480

ggcacagaat ttgtcgatgc cgtcgaagtt gatccgagca acagaaacca agaaacgagc    540

ggcacgtatc aaatccaagc gtggacgaaa tttgattttc cgggcagagg caatacgtat    600

agcagcttta aatggcgctg gtatcatttt gacggcacgg attgggatga aagcagaaaa    660

ctgaaccgga tctataaatt tcggagcacg ggcaaagcat gggattggga agtcgatacg    720

gaaaacggca actatgacta tctgatgttt gccgatctgg atatggatca tccggaagtc    780

gtcacggaac tgaaaaattg gggcacgtgg tatgttaata cgacgaacat cgatggcttt    840

agactggatg ccgtcaaaca tatcaaatat agcttttttc cggactggct gacgtatgtc    900

agaaaccaga cgggcaaaaa cctttttgcc gtcggcgaat tttggagcta tgacgtcaac    960

aaacttcata actatatcac gaaaacgaac ggcagcatga gcctttttga tgccccgctt   1020

cataacaact tttatacggc gagcaaaagc tcaggctatt ttgatatgag atatctgctg   1080

aacaacacgc tgatgaaaga tcaaccgagc ctggcagtca cactggtcga taaccatgat   1140

acacaaccgg gccaaagcct tcaaagctgg gtcgaaccgt ggtttaaacc gctggcgtat   1200

gcctttatcc tgacgagaca agaagggtat ccttgcgtct tttatggcga ctattatggc   1260

atcccgaaat ataatatccc gggcctgaaa agcaaaatcg atccgctgct gatcgccaga   1320

cgggattatg cctatggcac acagcgggat tatatcgacc atcaggacat catcggctgg   1380

acaagagaag gcatcgatac gaaaccgaat agcggactgg cagcactgat tacagatgga   1440

ccgggcggaa gcaaatggat gtatgtcggc aaaaaacatg ccggcaaagt cttttatgat   1500

ctgacgggca acagaagcga tacggtcacg atcaatgctg atggctgggg agaatttaaa   1560

gtcaatggcg gcagcgtttc aatctgggtc gccaaaacga gcaatgtcac gtttacggtc   1620

aacaatgcca cgacaacgag cggccaaaat gtctatgtcg tcgccaatat cccggaactg   1680

ggcaattgga atacggcgaa cgcaatcaaa atgaacccga gcagctatcc gacatggaaa   1740

gcgacaatcg ctctgccgca aggaaaagcg atcgaattta aatttatcaa aaaagaccag   1800

gcgggcaatg ttatttggga aagcacgagc aatagaacgt atacggtccc gtttagcagc   1860

acaggaagct atacagcgag ctggaatgtt ccgtgagtta acagaggacg gatttcctga   1920

aggaaatccg tttttttatt ttaagcttgg agacaaggta aaggataaaa cctcgag      1977


<210> 7
<211> 484
<212> PRT
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      polypeptide"

<400> 7
Asn Thr Ala Pro Ile Asn Glu Thr Met Met Gln Tyr Phe Glu Trp Asp 
1               5                   10                  15      


Leu Pro Asn Asp Gly Thr Leu Trp Thr Lys Val Lys Asn Glu Ala Ala 
            20                  25                  30          


Asn Leu Ser Ser Leu Gly Ile Thr Ala Leu Trp Leu Pro Pro Ala Tyr 
        35                  40                  45              


Lys Gly Thr Ser Gln Ser Asp Val Gly Tyr Gly Val Tyr Asp Leu Tyr 
    50                  55                  60                  


Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Ile Arg Thr Lys Tyr Gly 
65                  70                  75                  80  


Thr Lys Thr Gln Tyr Ile Gln Ala Ile Gln Ala Ala Lys Ala Ala Gly 
                85                  90                  95      


Met Gln Val Tyr Ala Asp Val Val Phe Asn His Lys Ala Gly Ala Asp 
            100                 105                 110         


Gly Thr Glu Phe Val Asp Ala Val Glu Val Asp Pro Ser Asn Arg Asn 
        115                 120                 125             


Gln Glu Thr Ser Gly Thr Tyr Gln Ile Gln Ala Trp Thr Lys Phe Asp 
    130                 135                 140                 


Phe Pro Gly Arg Gly Asn Thr Tyr Ser Ser Phe Lys Trp Arg Trp Tyr 
145                 150                 155                 160 


His Phe Asp Gly Thr Asp Trp Asp Glu Ser Arg Lys Leu Asn Arg Ile 
                165                 170                 175     


Tyr Lys Phe Arg Ser Thr Gly Lys Ala Trp Asp Trp Glu Val Asp Thr 
            180                 185                 190         


Glu Asn Gly Asn Tyr Asp Tyr Leu Met Phe Ala Asp Leu Asp Met Asp 
        195                 200                 205             


His Pro Glu Val Val Thr Glu Leu Lys Asn Trp Gly Thr Trp Tyr Val 
    210                 215                 220                 


Asn Thr Thr Asn Ile Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile 
225                 230                 235                 240 


Lys Tyr Ser Phe Phe Pro Asp Trp Leu Thr Tyr Val Arg Asn Gln Thr 
                245                 250                 255     


Gly Lys Asn Leu Phe Ala Val Gly Glu Phe Trp Ser Tyr Asp Val Asn 
            260                 265                 270         


Lys Leu His Asn Tyr Ile Thr Lys Thr Asn Gly Ser Met Ser Leu Phe 
        275                 280                 285             


Asp Ala Pro Leu His Asn Asn Phe Tyr Thr Ala Ser Lys Ser Ser Gly 
    290                 295                 300                 


Tyr Phe Asp Met Arg Tyr Leu Leu Asn Asn Thr Leu Met Lys Asp Gln 
305                 310                 315                 320 


Pro Ser Leu Ala Val Thr Leu Val Asp Asn His Asp Thr Gln Pro Gly 
                325                 330                 335     


Gln Ser Leu Gln Ser Trp Val Glu Pro Trp Phe Lys Pro Leu Ala Tyr 
            340                 345                 350         


Ala Phe Ile Leu Thr Arg Gln Glu Gly Tyr Pro Cys Val Phe Tyr Gly 
        355                 360                 365             


Asp Tyr Tyr Gly Ile Pro Lys Tyr Asn Ile Pro Gly Leu Lys Ser Lys 
    370                 375                 380                 


Ile Asp Pro Leu Leu Ile Ala Arg Arg Asp Tyr Ala Tyr Gly Thr Gln 
385                 390                 395                 400 


Arg Asp Tyr Ile Asp His Gln Asp Ile Ile Gly Trp Thr Arg Glu Gly 
                405                 410                 415     


Ile Asp Thr Lys Pro Asn Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly 
            420                 425                 430         


Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Lys Lys His Ala Gly Lys 
        435                 440                 445             


Val Phe Tyr Asp Leu Thr Gly Asn Arg Ser Asp Thr Val Thr Ile Asn 
    450                 455                 460                 


Ala Asp Gly Trp Gly Glu Phe Lys Val Asn Gly Gly Ser Val Ser Ile 
465                 470                 475                 480 


Trp Val Ala Lys 
                


<210> 8
<211> 29
<212> DNA
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      primer"

<400> 8
ctcattctgc agcttcagca aatacggcg                                       29


<210> 9
<211> 35
<212> DNA
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      primer"

<400> 9
ctctgttaac tcatttggcg acccagattg aaacg                                35


<210> 10
<211> 31
<212> DNA
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      primer"

<400> 10
ctataaattt acgggcaaag catgggattg g                                    31


<210> 11
<211> 33
<212> DNA
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      primer"

<400> 11
tgctttgccc gtaaatttat agatccggtt cag                                  33


<210> 12
<211> 28
<212> DNA
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      primer"

<400> 12
ctatgactat ctgctgtttg ccgatctg                                        28


<210> 13
<211> 28
<212> DNA
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      primer"

<400> 13
cagatcggca aacagcagat agtcatag                                        28


<210> 14
<211> 58
<212> DNA
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      primer"

<400> 14
gcatgggatt gggaagtcga tacggaaaac ggcaactatg actatctgct gtttgccg       58


<210> 15
<211> 52
<212> DNA
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      primer"

<400> 15
cgtatcgact tcccaatccc atgctttgcc cgtaaattta tagatccggt tc             52


<210> 16
<211> 87
<212> DNA
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      oligonucleotide"

<400> 16
atgaaacaac aaaaacggct ttacgcccga ttgctgacgc tgttatttgc gctcatcttc     60

ttgctgcctc attctgcagc ttcagca                                         87


<210> 17
<211> 29
<212> PRT
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      peptide"

<400> 17
Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
1               5                   10                  15      


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ser Ala 
            20                  25                  


<210> 18
<211> 430
<212> PRT
<213> Artificial sequence

<220>
<221> source
<223> /note="Description of artificial sequence: Synthetic 
      polypeptide"

<400> 18
Met Asp Gln Ala Gly Lys Ser Pro Ala Gly Val Arg Tyr His Gly Gly 
1               5                   10                  15      


Asp Glu Ile Ile Leu Gln Gly Phe His Trp Asn Val Val Arg Glu Ala 
            20                  25                  30          


Pro Tyr Asn Trp Tyr Asn Ile Leu Arg Gln Gln Ala Ser Thr Ile Ala 
        35                  40                  45              


Ala Asp Gly Phe Ser Ala Ile Trp Met Pro Val Pro Trp Arg Asp Phe 
    50                  55                  60                  


Ser Ser Trp Thr Asp Gly Asp Lys Ser Gly Gly Gly Glu Gly Tyr Phe 
65                  70                  75                  80  


Trp His Asp Phe Asn Lys Asn Gly Arg Tyr Gly Ser Asp Ala Gln Leu 
                85                  90                  95      


Arg Gln Ala Ala Gly Ala Leu Gly Gly Ala Gly Val Lys Val Leu Tyr 
            100                 105                 110         


Asp Val Val Pro Asn His Met Asn Arg Phe Tyr Pro Asp Lys Glu Ile 
        115                 120                 125             


Asn Leu Pro Ala Gly Gln Arg Phe Trp Arg Asn Asp Cys Pro Asp Pro 
    130                 135                 140                 


Gly Asn Gly Pro Asn Asp Cys Asp Asp Gly Asp Arg Phe Leu Gly Gly 
145                 150                 155                 160 


Glu Ala Asp Leu Asn Thr Gly His Pro Gln Ile Tyr Gly Met Phe Arg 
                165                 170                 175     


Asp Glu Phe Thr Asn Leu Arg Ser Gly Tyr Gly Ala Gly Gly Phe Arg 
            180                 185                 190         


Phe Asp Phe Val Arg Gly Tyr Ala Pro Glu Arg Val Asp Ser Trp Met 
        195                 200                 205             


Ser Asp Ser Ala Asp Ser Ser Phe Cys Val Gly Glu Leu Trp Lys Glu 
    210                 215                 220                 


Pro Ser Glu Tyr Pro Pro Trp Asp Trp Arg Asn Thr Ala Ser Trp Gln 
225                 230                 235                 240 


Gln Ile Ile Lys Asp Trp Ser Asp Arg Ala Lys Cys Pro Val Phe Asp 
                245                 250                 255     


Phe Ala Leu Lys Glu Arg Met Gln Asn Gly Ser Val Ala Asp Trp Lys 
            260                 265                 270         


His Gly Leu Asn Gly Asn Pro Asp Pro Arg Trp Arg Glu Val Ala Val 
        275                 280                 285             


Thr Phe Val Asp Asn His Asp Thr Gly Tyr Ser Pro Gly Gln Asn Gly 
    290                 295                 300                 


Gly Gln His Lys Trp Pro Leu Gln Asp Gly Leu Ile Arg Gln Ala Tyr 
305                 310                 315                 320 


Ala Tyr Ile Leu Thr Ser Pro Gly Thr Pro Val Val Tyr Trp Pro His 
                325                 330                 335     


Met Tyr Asp Trp Gly Tyr Gly Asp Phe Ile Arg Gln Leu Ile Gln Val 
            340                 345                 350         


Arg Arg Thr Ala Gly Val Arg Ala Asp Ser Ala Ile Ser Phe His Ser 
        355                 360                 365             


Gly Tyr Ser Gly Leu Val Ala Thr Val Ser Gly Ser Gln Gln Thr Leu 
    370                 375                 380                 


Val Val Ala Leu Asn Ser Asp Leu Ala Asn Pro Gly Gln Val Ala Ser 
385                 390                 395                 400 


Gly Ser Phe Ser Glu Ala Val Asn Ala Ser Asn Gly Gln Val Arg Val 
                405                 410                 415     


Trp Arg Ser Gly Ser Gly Asp Gly Gly Gly Asn Asp Gly Gly 
            420                 425                 430 



