                              Sequence Listing

<110> GENENTECH, INC.
      Lebreton, Benedicte Andree
      O'Connor, Deborah Ann
      Safta, Aurelia
      Sharma, Mandakini

<120> ANTIBODY PURIFICATION BY CATION EXCHANGE CHROMATOGRAPHY

<130> P2280R1 WO


<150> US 60/983,825
<151> 2007-10-30

<160> 20

<210> 1
<211> 432
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 1
 Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly
   1               5                  10                  15

 Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr
                  20                  25                  30

 Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly Arg Gly Leu
                  35                  40                  45

 Glu Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr
                  50                  55                  60

 Asn Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser
                  65                  70                  75

 Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp
                  80                  85                  90

 Ser Ala Val Tyr Tyr Cys Ala Arg Ser Thr Tyr Tyr Gly Gly Asp
                  95                 100                 105

 Trp Tyr Phe Asn Val Trp Gly Ala Gly Thr Thr Val Thr Val Ser
                 110                 115                 120

 Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser
                 125                 130                 135

 Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val
                 140                 145                 150

 Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
                 155                 160                 165

 Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser
                 170                 175                 180

 Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
                 185                 190                 195

 Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro
                 200                 205                 210

 Ser Asn Thr Lys Val Asp Lys Lys Ala Glu Pro Lys Ser Cys Asp
                 215                 220                 225

 Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly
                 230                 235                 240

 Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu
                 245                 250                 255

 Met Ile Ser Arg Thr Pro Glu Val Thr Asp Gly Val Glu Val His
                 260                 265                 270

 Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
                 275                 280                 285

 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn
                 290                 295                 300

 Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
                 305                 310                 315

 Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
                 320                 325                 330

 Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys
                 335                 340                 345

 Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser
                 350                 355                 360

 Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn
                 365                 370                 375

 Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
                 380                 385                 390

 Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly
                 395                 400                 405

 Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His
                 410                 415                 420

 Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
                 425                 430        

<210> 2
<211> 213
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 2
 Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser Ala Ser Pro
   1               5                  10                  15

 Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser
                  20                  25                  30

 Tyr Ile His Trp Phe Gln Gln Lys Pro Gly Ser Ser Pro Lys Pro
                  35                  40                  45

 Trp Ile Tyr Ala Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg
                  50                  55                  60

 Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser
                  65                  70                  75

 Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp
                  80                  85                  90

 Thr Ser Asn Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile
                  95                 100                 105

 Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser
                 110                 115                 120

 Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu
                 125                 130                 135

 Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp
                 140                 145                 150

 Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln
                 155                 160                 165

 Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu
                 170                 175                 180

 Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val
                 185                 190                 195

 Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg
                 200                 205                 210

 Gly Glu Cys
            

<210> 3
<211> 121
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 3
 Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly
   1               5                  10                  15

 Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr
                  20                  25                  30

 Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly Arg Gly Leu
                  35                  40                  45

 Glu Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr
                  50                  55                  60

 Asn Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser
                  65                  70                  75

 Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp
                  80                  85                  90

 Ser Ala Val Tyr Tyr Cys Ala Arg Ser Thr Tyr Tyr Gly Gly Asp
                  95                 100                 105

 Trp Tyr Phe Asn Val Trp Gly Ala Gly Thr Thr Val Thr Val Ser
                 110                 115                 120

 Ala
    

<210> 4
<211> 106
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 4
 Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser Ala Ser Pro
   1               5                  10                  15

 Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser
                  20                  25                  30

 Tyr Ile His Trp Phe Gln Gln Lys Pro Gly Ser Ser Pro Lys Pro
                  35                  40                  45

 Trp Ile Tyr Ala Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg
                  50                  55                  60

 Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser
                  65                  70                  75

 Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp
                  80                  85                  90

 Thr Ser Asn Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile
                  95                 100                 105

 Lys
    

<210> 5
<211> 5
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 5
 Ser Tyr Asn Met His
                   5

<210> 6
<211> 17
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 6
 Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe
   1               5                  10                  15

 Lys Gly
        

<210> 7
<211> 12
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 7
 Ser Thr Tyr Tyr Gly Gly Asp Trp Tyr Phe Asn Val
                   5                  10        

<210> 8
<211> 10
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 8
 Arg Ala Ser Ser Ser Val Ser Tyr Ile His
                   5                  10

<210> 9
<211> 7
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 9
 Ala Thr Ser Asn Leu Ala Ser
                   5        

<210> 10
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 10
 Gln Gln Trp Thr Ser Asn Pro Pro Thr
                   5                

<210> 11
<211> 453
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 11
 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
   1               5                  10                  15

 Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Phe Thr
                  20                  25                  30

 Asn Tyr Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
                  35                  40                  45

 Glu Trp Val Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr
                  50                  55                  60

 Ala Ala Asp Phe Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser
                  65                  70                  75

 Lys Ser Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp
                  80                  85                  90

 Thr Ala Val Tyr Tyr Cys Ala Lys Tyr Pro His Tyr Tyr Gly Ser
                  95                 100                 105

 Ser His Trp Tyr Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr
                 110                 115                 120

 Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
                 125                 130                 135

 Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys
                 140                 145                 150

 Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
                 155                 160                 165

 Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu
                 170                 175                 180

 Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro
                 185                 190                 195

 Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His
                 200                 205                 210

 Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser
                 215                 220                 225

 Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu
                 230                 235                 240

 Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
                 245                 250                 255

 Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val
                 260                 265                 270

 Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val
                 275                 280                 285

 Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu
                 290                 295                 300

 Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
                 305                 310                 315

 His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
                 320                 325                 330

 Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala
                 335                 340                 345

 Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser
                 350                 355                 360

 Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
                 365                 370                 375

 Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn
                 380                 385                 390

 Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
                 395                 400                 405

 Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys
                 410                 415                 420

 Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His
                 425                 430                 435

 Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
                 440                 445                 450

 Pro Gly Lys
            

<210> 12
<211> 214
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 12
 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
   1               5                  10                  15

 Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser
                  20                  25                  30

 Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys
                  35                  40                  45

 Val Leu Ile Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser
                  50                  55                  60

 Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile
                  65                  70                  75

 Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln
                  80                  85                  90

 Tyr Ser Thr Val Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu
                  95                 100                 105

 Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro
                 110                 115                 120

 Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu
                 125                 130                 135

 Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val
                 140                 145                 150

 Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu
                 155                 160                 165

 Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr
                 170                 175                 180

 Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu
                 185                 190                 195

 Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn
                 200                 205                 210

 Arg Gly Glu Cys
                

<210> 13
<211> 123
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 13
 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly
   1               5                  10                  15

 Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Phe Thr
                  20                  25                  30

 Asn Tyr Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu
                  35                  40                  45

 Glu Trp Val Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr
                  50                  55                  60

 Ala Ala Asp Phe Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser
                  65                  70                  75

 Lys Ser Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp
                  80                  85                  90

 Thr Ala Val Tyr Tyr Cys Ala Lys Tyr Pro His Tyr Tyr Gly Ser
                  95                 100                 105

 Ser His Trp Tyr Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr
                 110                 115                 120

 Val Ser Ser
            

<210> 14
<211> 108
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 14
 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
   1               5                  10                  15

 Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser
                  20                  25                  30

 Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys
                  35                  40                  45

 Val Leu Ile Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser
                  50                  55                  60

 Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile
                  65                  70                  75

 Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln
                  80                  85                  90

 Tyr Ser Thr Val Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu
                  95                 100                 105

 Ile Lys Arg
            

<210> 15
<211> 10
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 15
 Gly Tyr Thr Phe Thr Asn Tyr Gly Met Asn
                   5                  10

<210> 16
<211> 17
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 16
 Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Ala Asp Phe
   1               5                  10                  15

 Lys Arg
        

<210> 17
<211> 13
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 17
 Pro His Tyr Tyr Gly Ser Ser His Trp Tyr Phe Asp Val
                   5                  10            

<210> 18
<211> 11
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 18
 Ser Ala Ser Gln Asp Ile Ser Asn Tyr Leu Asn
                   5                  10    

<210> 19
<211> 7
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 19
 Phe Thr Ser Ser Leu His Ser
                   5        

<210> 20
<211> 9
<212> PRT
<213> Artificial sequence

<220>
<223> sequence is synthesized

<400> 20
 Gln Gln Tyr Ser Thr Val Pro Trp Thr
                   5                

