
                                SEQUENCE LISTING

<110> Maxygen, Inc.
      Karrer, Erik E.
      Paidhungat, Madan M.
      Bass, Steven H.
      Neighbors, Margaret
      Punnonen, Juha
      Chapin, Steven J.
      Viswanathan, Sridhar 
      Larsen, Brent R.

<120> IMMUNOSUPPRESSIVE POLYPEPTIDES AND NUCLEIC ACIDS

<130> 0364.210WO

<150> US 60/984,631       
<151> 2007-11-01  

<150> US 61/051,215       
<151> 2008-05-07  

<160> 228

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-1

<400> 1
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 2
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-2

<400> 2
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Thr Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 3
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-3

<400> 3
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 4
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-4

<400> 4
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 5
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-5

<400> 5
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Ala Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 6
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-6

<400> 6
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Met Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 7
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-7

<400> 7
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 8
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-8

<400> 8
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Ser
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 9
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-9

<400> 9
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 10
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-11

<400> 10
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 11
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-12

<400> 11
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 12
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-14

<400> 12
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 13
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-15

<400> 13
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 14
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-16

<400> 14
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 15
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-17

<400> 15
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 16
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-19

<400> 16
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 17
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-20

<400> 17
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 18
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-21

<400> 18
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 19
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-22

<400> 19
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 20
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-23

<400> 20
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 21
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-24

<400> 21
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 22
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-25

<400> 22
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 23
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-26

<400> 23
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 24
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-27

<400> 24
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 25
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-28

<400> 25
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 26
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-29

<400> 26
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 27
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-30

<400> 27
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 28
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-31

<400> 28
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 29
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-32

<400> 29
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Lys Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 30
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-33

<400> 30
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Lys Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 31
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-34

<400> 31
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 32
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-39

<400> 32
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 33
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-50

<400> 33
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 34
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-52

<400> 34
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 35
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-53

<400> 35
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 36
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-54

<400> 36
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 37
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-55

<400> 37
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 38
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-56

<400> 38
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 39
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-57

<400> 39
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 40
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-58

<400> 40
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 41
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-59

<400> 41
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 42
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-60

<400> 42
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 43
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-61

<400> 43
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 44
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-62

<400> 44
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 45
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-63

<400> 45
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Ala Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 46
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-64

<400> 46
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Met Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 47
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-65

<400> 47
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 48
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-66

<400> 48
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 49
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-68

<400> 49
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 50
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-69

<400> 50
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 51
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-70

<400> 51
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 52
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-71

<400> 52
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 53
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-72

<400> 53
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 54
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-73

<400> 54
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 55
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-74

<400> 55
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 56
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-75

<400> 56
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 57
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-76

<400> 57
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 58
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D1

<400> 58
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro His Lys Ala Thr Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asn Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Phe Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 59
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D1T

<400> 59
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro His Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asn Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Phe Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 60
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D2

<400> 60
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro His Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asn Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Phe Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Met Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 61
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3

<400> 61
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 62
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D4

<400> 62
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ser Ser Pro Gly Lys Ala Asp Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Ser Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Glu Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Thr Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Val Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 63
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D5

<400> 63
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Thr Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Ala Thr Tyr Met Met Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 64
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D6

<400> 64
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Thr Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Met Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 65
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D20

<400> 65
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro His Lys Ala Thr Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Met Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 66
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D21

<400> 66
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ser Ala Pro His Asn Ala Asp Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Lys Ala Ser Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Glu Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Thr Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Val Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Phe Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 67
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D23

<400> 67
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ser Ala Pro His Asn Ala Ala Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Lys Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Leu Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 68
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D24

<400> 68
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Lys Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Leu Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 69
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D26

<400> 69
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ala Pro His Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Glu Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Val Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Leu Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 70
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D27

<400> 70
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Glu Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Val Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Leu Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 71
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D28

<400> 71
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ala Pro His Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Met Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Leu Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 72
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D29

<400> 72
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ala Pro His Lys Ala Asp Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Lys Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Met Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Leu Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 73
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D31

<400> 73
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ala Pro His Asn Ala Asp Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Val Glu Asp Glu Leu Thr Phe Leu Asp Lys Ser
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 74
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-12-IgG2 fusion protein (with C-terminal lysine)

<400> 74
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 75
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-14-IgG2 fusion protein (with C-terminal lysine)

<400> 75
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 76
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-17-IgG2 fusion protein (with C-terminal lysine)

<400> 76
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 77
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-20-IgG2 fusion protein (with C-terminal lysine)

<400> 77
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 78
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-27-IgG2 fusion protein (with C-terminal lysine)

<400> 78
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 79
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-29-IgG2 fusion protein (with C-terminal lysine)

<400> 79
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 80
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-1 coding sequence 

<400> 80
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 81
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-2 coding sequence

<400> 81
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccacc gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 82
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-3 coding sequence

<400> 82
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 83
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-4 coding sequence

<400> 83
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 84
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-5 coding sequence

<400> 84
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctgcg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 85
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-6 coding sequence

<400> 85
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga tgggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 86
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-7 coding sequence

<400> 86
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 87
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-8 coding sequence

<400> 87
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatt ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 88
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-9 coding sequence

<400> 88
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 89
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-11 coding sequence

<400> 89
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 90
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-12 coding sequence

<400> 90
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 91
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-14 coding sequence

<400> 91
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 92
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant

<400> 92
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 93
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-16 coding sequence

<400> 93
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 94
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-17 coding sequence

<400> 94
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 95
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-19 coding sequence

<400> 95
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 96
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-20 coding sequence

<400> 96
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 97
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-21 coding sequence

<400> 97
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 98
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-22 coding sequence

<400> 98
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 99
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-23 coding sequence

<400> 99
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 100
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-24 coding sequence

<400> 100
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 101
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-25 coding sequence

<400> 101
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 102
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-26 coding sequence

<400> 102
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 103
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-27 coding sequence

<400> 103
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 104
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-28 coding sequence

<400> 104
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
ctcgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 105
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-29 coding sequence

<400> 105
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 106
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-30 coding sequence

<400> 106
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 107
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-31 coding sequence

<400> 107
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 108
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-32 coding sequence

<400> 108
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgcaaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 109
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-33 coding sequence

<400> 109
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgcaaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 110
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-34 coding sequence

<400> 110
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 111
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-39 coding sequence

<400> 111
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 112
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-50 coding sequence

<400> 112
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 113
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-52 coding sequence

<400> 113
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 114
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-53 coding sequence

<400> 114
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacg agggtatagg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 115
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-54 coding sequence

<400> 115
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaaaatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 116
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-55 coding sequence

<400> 116
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaaaatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 117
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-56 coding sequence

<400> 117
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 118
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-57 coding sequence

<400> 118
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaagatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 119
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-58 coding sequence

<400> 119
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 120
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-59 coding sequence

<400> 120
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaaaatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 121
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-60 coding sequence

<400> 121
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 122
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-61 coding sequence

<400> 122
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 123
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-62 coding sequence

<400> 123
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 124
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-63 coding sequence

<400> 124
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctgcg acatacatga aggaaaatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 125
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-64 coding sequence

<400> 125
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga tggaaaatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 126
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-65 coding sequence

<400> 126
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacg agggtatagg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 127
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-66 coding sequence

<400> 127
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacg agggtatagg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 128
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-68 coding sequence

<400> 128
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacg agggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 129
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-69 coding sequence

<400> 129
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacg agggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 130
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-70 coding sequence

<400> 130
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacg agggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 131
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-71 coding sequence

<400> 131
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacg agggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 132
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-72 coding sequence

<400> 132
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacg agggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 133
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-73 coding sequence

<400> 133
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacg agggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 134
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-74 coding sequence

<400> 134
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga agggaaatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacg agggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 135
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-75 coding sequence

<400> 135
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaaaatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 136
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3-76 coding sequence

<400> 136
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg cgtcacccgg caaagccaac gaagttagag tcacagtgct ccgccaagcc 120
gatagtcaag ttacagaagt ctgcgctatg acatacatga aggaagatga actaaccttc 180
cttgacgatc ccatctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggtattgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 137
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D1 coding sequence

<400> 137
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg agtcacccca taaagccacc gaaattagag tcacagtgct ccgccaagcc 120
aacagtcaag tgacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
ctggacgatc ccttctgcac aggtaccttt tccggaaatc aggttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atctgtaaag tcgaactcat gtaccccccc 300
ccttactacc tgggtatagg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 138
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D1T coding sequence

<400> 138
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg agtcacccca taaagccaac gaaattagag tcacagtgct ccgccaagcc 120
aacagtcaag tgacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
ctggacgatc ccttctgcac aggtaccttt tccggaaatc aggttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atctgtaaag tcgaactcat gtaccccccc 300
ccttactacc tgggtatagg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 139
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D2 coding sequence

<400> 139
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg agtcacccca taaagccaac gaaattagag tcacagtgct ccgccaagcc 120
aacagtcaag tgacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
ctggacgatc ccttctgcac aggtaccttt tccggaaatc aggttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atctgtaaag tcgaactcat gtatcccccc 300
ccttactacc tgggtatggg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 140
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D3 coding sequence

<400> 140
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgccaagcc 120
ggaagtcaag ttacagaagt ctgcgctatg acatacatga agggagatga actaaccttc 180
cttgacgatc ccagctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgcggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactact tgggttttgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 141
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D4 coding sequence

<400> 141
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatata gctcacccgg gaaagccgac gaaattagag tcacagtgct ccgccaagcc 120
tctagtcaag tcacagaagt ctgcgctatg acatacatgg agggagatga actaaccttc 180
cttgacgacc ccacctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgtcgatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc tgggtttcgg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 142
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D5 coding sequence

<400> 142
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg catcccccgg aaaagccact gaagttagag tcacagtcct ccgccaggct 120
gattcacaag taacagaagt ctgcgctgca acatatatga tgggcgacga actcaccttc 180
ctcgacgacc caatttgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag caatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc ttggtatagg taatggcact cagatctacg tcattgaccc cgaaccctgc 360
cctgattctg at                                                     372

<210> 143
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D6 coding sequence

<400> 143
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg catcccccgg aaaagccact gaaattagag tcacagtcct ccgccaggct 120
gattcacaag taacagaagt ctgcgctatg acatatatga tgggcgacga actcaccttc 180
ctcgacgacc caatttgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag caatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc ttggtatagg taatggcact cagatctacg tcattgaccc cgaaccctgc 360
cctgattctg at                                                     372

<210> 144
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D20 coding sequence

<400> 144
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg agtcccccca caaagccact gaaattagag tcacagtgct ccgccaggct 120
gattcacaag taacagaagt ctgcgctatg acatatatga tggaggacga actcaccttc 180
ctcgacgacc caatttgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag caatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc ttggtatagg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 145
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D21 coding sequence

<400> 145
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatt ccgcccccca caacgccgac gaaatcagag tcacagtcct ccgcaaagct 120
tcttcacaag taacagaagt ctgcgctatg acatatatgg aagaagatga actcaccttc 180
ctcgacgacc ccacctgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag ccgtcgatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc tgggtttcgg taatggcact cagatctacg tcattgaccc cgaaccctgc 360
cctgattctg at                                                     372

<210> 146
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D23 coding sequence

<400> 146
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatt ccgcccccca caacgccgcc gaaatcagag tcacagtcct ccgcaaagct 120
ggttcacaag taacagaagt ctgcgctatg acatatatga aagaagacga actcaccttc 180
ctcgacgacc cgtcctgcac aggtaccttc tccggaaatc aagttaatct tactattcaa 240
ggacttcgag cagccgatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc ttggtctagg taatggcact cagatctacg tcattgaccc cgaaccctgc 360
cctgattctg at                                                     372

<210> 147
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D24 coding sequence

<400> 147
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtg 60
tgtgaatatg agtcacccgg caaagccaac gaaattagag tcacagtgct ccgcaaagct 120
ggttcacaag taacagaagt ctgcgctatg acatatatga aagaagacga actcaccttc 180
ctcgacgacc cgtcctgcac aggtaccttc tccggaaatc aagttaatct tactattcaa 240
ggacttcgag cagccgatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc ttggtctagg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 148
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D26 coding sequence

<400> 148
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg aggcccccca caaagccaac gaagttagag tcacagtcct ccgcgaagct 120
ggttcacaag taacagaagt ctgcgctatg acatatatgg tcgaggacga actcaccttc 180
ctcgacgacc cgtcctgcac aggtaccttc tccggaaatc aagttaatct tactattcaa 240
ggacttcgag cagccgatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc ttggtctagg taatggcact cagatctacg tcattgaccc cgaaccctgc 360
cctgattctg at                                                     372

<210> 149
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D27 coding sequence

<400> 149
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg agtcacccgg caaagccaac gaagttagag tcacagtcct ccgcgaagct 120
ggttcacaag taacagaagt ctgcgctatg acatatatgg tcgaggacga actcaccttc 180
ctcgacgacc cgtcctgcac aggtaccttc tccggaaatc aagttaatct tactattcaa 240
ggacttcgag cagccgatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc ttggtctagg taatggcact cagatctacg tcattgaccc cgaaccctgc 360
cctgattctg at                                                     372

<210> 150
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D28 coding sequence

<400> 150
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg aggcccccca caaagccaac gaagttagag tcacagtcct ccgccaagct 120
ggatcacaag taacagaagt ctgcgctatg acatatatga tggaggacga actcaccttc 180
ctcgacgacc cgtcctgcac aggtaccttc tccggaaatc aagttaatct tactattcaa 240
ggacttcgag cagccgatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc ttggtctagg taatggcact cagatctacg tcattgaccc cgaaccctgc 360
cctgattctg at                                                     372

<210> 151
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D29 coding sequence

<400> 151
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg aggcccccca caaagccgat gaaattagag tcacagtcct ccgcaaggct 120
gattcacaag taacagaagt ctgcgctatg acatatatga tggaggacga actcaccttc 180
ctcgacgacc cgtcctgcac aggtaccttc tccggaaatc aagttaatct tactattcaa 240
ggacttcgag caatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc ttggtctagg taatggcact cagatctacg tcattgaccc cgaaccctgc 360
cctgattctg at                                                     372

<210> 152
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> CTLA-4 ECD mutant D31 coding sequence

<400> 152
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg aggcccccca caacgccgat gaaatcagag tcacagtcct ccgccaagct 120
ggttcacaag taacagaagt ctgcgctatg acatatatgg tcgaggacga actcaccttc 180
ctcgacaagt caatttgcac aggtaccttt tccggaaatc aagttaatct tactattcaa 240
ggacttcgag caatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacc ttggtatagg taatggcact cagatctacg tcattgaccc cgaaccctgc 360
cctgattctg at                                                     372

<210> 153
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-12-IgG2 fusion protein coding sequence

<400> 153
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gagtcacccg gcaaagccaa cgaaattaga gtcacagtgc tccgccaagc cggaagtcaa 240
gttacagaag tctgcgctat gacatacatg aagggagatg aactaacctt ccttgacgat 300
cccagctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccgcggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
ttgggtattg gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 154
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-14-IgG2 fusion protein coding sequence

<400> 154
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gagtcacccg gcaaagccaa cgaaattaga gtcacagtgc tccgccaagc cgatagtcaa 240
gttacagaag tctgcgctat gacatacatg aagggagatg aactaacctt ccttgacgat 300
cccagctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccgcggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
ttgggtattg gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 155
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-17-IgG2 fusion protein coding sequence

<400> 155
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gagtcacccg gcaaagccaa cgaaattaga gtcacagtgc tccgccaagc cgatagtcaa 240
gttacagaag tctgcgctat gacatacatg aagggagatg aactaacctt ccttgacgat 300
cccagctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
ttgggtattg gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 156
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-20-IgG2 fusion protein coding sequence

<400> 156
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gagtcacccg gcaaagccaa cgaaattaga gtcacagtgc tccgccaagc cgatagtcaa 240
gttacagaag tctgcgctat gacatacatg aagggaaatg aactaacctt ccttgacgat 300
cccagctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
ttgggtattg gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 157
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-27-IgG2 fusion protein coding sequence

<400> 157
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gagtcacccg gcaaagccaa cgaagttaga gtcacagtgc tccgccaagc cgatagtcaa 240
gttacagaag tctgcgctat gacatacatg aagggaaatg aactaacctt ccttgacgat 300
cccagctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
ttgggtattg gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 158
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-29-IgG2 fusion protein coding sequence

<400> 158
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gcgtcacccg gcaaagccaa cgaaattaga gtcacagtgc tccgccaagc cgatagtcaa 240
gttacagaag tctgcgctat gacatacatg aagggagatg aactaacctt ccttgacgat 300
cccagctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
ttgggtattg gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 159
<211> 124
<212> PRT
<213> Homo sapiens

<220> 
<223> wild-type human CTLA-4 ECD

<400> 159
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Thr Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr Phe Leu Asp Asp Ser
    50                  55                  60                  
Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 160
<211> 223
<212> PRT
<213> Homo Sapiens

<220> 
<223> wild-type human full-length CTLA-4 

<400> 160
Met Ala Cys Leu Gly Phe Gln Arg His Lys Ala Gln Leu Asn Leu Ala
 1               5                  10                  15      
Thr Arg Thr Trp Pro Cys Thr Leu Leu Phe Phe Leu Leu Phe Ile Pro
            20                  25                  30          
Val Phe Cys Lys Ala Met His Val Ala Gln Pro Ala Val Val Leu Ala
        35                  40                  45              
Ser Ser Arg Gly Ile Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly
    50                  55                  60                  
Lys Ala Thr Glu Val Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln
65                  70                  75                  80  
Val Thr Glu Val Cys Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr
                85                  90                  95      
Phe Leu Asp Asp Ser Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val
            100                 105                 110         
Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile
        115                 120                 125             
Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly
    130                 135                 140                 
Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser
145                 150                 155                 160 
Asp Phe Leu Leu Trp Ile Leu Ala Ala Val Ser Ser Gly Leu Phe Phe
                165                 170                 175     
Tyr Ser Phe Leu Leu Thr Ala Val Ser Leu Ser Lys Met Leu Lys Lys
            180                 185                 190         
Arg Ser Pro Leu Thr Thr Gly Val Tyr Val Lys Met Pro Pro Thr Glu
        195                 200                 205             
Pro Glu Cys Glu Lys Gln Phe Gln Pro Tyr Phe Ile Pro Ile Asn
    210                 215                 220             


<210> 161
<211> 389
<212> PRT
<213> Artificial Sequence

<220> 
<223> predicted WT human CTLA4-IgG2 fusion protein

<400> 161
Met Ala Cys Leu Gly Phe Gln Arg His Lys Ala Gln Leu Asn Leu Ala
 1               5                  10                  15      
Thr Arg Thr Trp Pro Cys Thr Leu Leu Phe Phe Leu Leu Phe Ile Pro
            20                  25                  30          
Val Phe Cys Lys Ala Met His Val Ala Gln Pro Ala Val Val Leu Ala
        35                  40                  45              
Ser Ser Arg Gly Ile Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly
    50                  55                  60                  
Lys Ala Thr Glu Val Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln
65                  70                  75                  80  
Val Thr Glu Val Cys Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr
                85                  90                  95      
Phe Leu Asp Asp Ser Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val
            100                 105                 110         
Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile
        115                 120                 125             
Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly
    130                 135                 140                 
Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser
145                 150                 155                 160 
Asp Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro
                165                 170                 175     
Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
            180                 185                 190         
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
        195                 200                 205             
Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
    210                 215                 220                 
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
225                 230                 235                 240 
Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu
                245                 250                 255     
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala
            260                 265                 270         
Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro
        275                 280                 285             
Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln
    290                 295                 300                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
305                 310                 315                 320 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                325                 330                 335     
Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
            340                 345                 350         
Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
        355                 360                 365             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    370                 375                 380                 
Leu Ser Pro Gly Lys
385                 


<210> 162
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> WT human mature CTLA4-IgG2 fusion protein

<400> 162
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Thr Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr Phe Leu Asp Asp Ser
    50                  55                  60                  
Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 163
<211> 1167
<212> DNA
<213> Artificial Sequence

<220> 
<223> WT human CTLA4-IgG2 fusion protein coding sequence

<400> 163
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt ttgtgaatat 180
gcatcccccg gaaaagccac tgaagttaga gtcacagtcc tccgccaggc tgattcacaa 240
gtaacagaag tctgcgctgc aacatatatg atgggcaacg aactcacctt cctcgacgac 300
tcaatttgca caggtacctc ctccggaaat caagttaatc ttactattca aggacttcga 360
gcaatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
cttggtatag gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaa                                     1167

<210> 164
<211> 357
<212> PRT
<213> Artificial Sequence

<220> 
<223> Orencia(R) (Bristol-Myers Squibb Co.)

<400> 164
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Thr Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr Phe Leu Asp Asp Ser
    50                  55                  60                  
Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Gln Glu Pro Lys
        115                 120                 125             
Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu
    130                 135                 140                 
Leu Gly Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
145                 150                 155                 160 
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
                165                 170                 175     
Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
            180                 185                 190         
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
        195                 200                 205             
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
    210                 215                 220                 
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
225                 230                 235                 240 
Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
                245                 250                 255     
Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln
            260                 265                 270         
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
        275                 280                 285             
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
    290                 295                 300                 
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
305                 310                 315                 320 
Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
                325                 330                 335     
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
            340                 345                 350         
Leu Ser Pro Gly Lys
        355         


<210> 165
<211> 394
<212> PRT
<213> Artificial Sequence

<220> 
<223> predicted LEA29Y-Ig fusion protein 

<400> 165
Met Ala Cys Leu Gly Phe Gln Arg His Lys Ala Gln Leu Asn Leu Ala
 1               5                  10                  15      
Thr Arg Thr Trp Pro Cys Thr Leu Leu Phe Phe Leu Leu Phe Ile Pro
            20                  25                  30          
Val Phe Cys Lys Ala Met His Val Ala Gln Pro Ala Val Val Leu Ala
        35                  40                  45              
Ser Ser Arg Gly Ile Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly
    50                  55                  60                  
Lys Tyr Thr Glu Val Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln
65                  70                  75                  80  
Val Thr Glu Val Cys Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr
                85                  90                  95      
Phe Leu Asp Asp Ser Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val
            100                 105                 110         
Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile
        115                 120                 125             
Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly
    130                 135                 140                 
Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser
145                 150                 155                 160 
Asp Gln Glu Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser
                165                 170                 175     
Pro Ala Pro Glu Leu Leu Gly Gly Ser Ser Val Phe Leu Phe Pro Pro
            180                 185                 190         
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
        195                 200                 205             
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
    210                 215                 220                 
Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
225                 230                 235                 240 
Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
                245                 250                 255     
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
            260                 265                 270         
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
        275                 280                 285             
Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
    290                 295                 300                 
Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
305                 310                 315                 320 
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
                325                 330                 335     
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
            340                 345                 350         
Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
        355                 360                 365             
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
    370                 375                 380                 
Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
385                 390                 


<210> 166
<211> 357
<212> PRT
<213> Artificial Sequence

<220> 
<223> mature LEA29Y-Ig fusion protein

<400> 166
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Tyr Thr Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr Phe Leu Asp Asp Ser
    50                  55                  60                  
Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Gln Glu Pro Lys
        115                 120                 125             
Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu
    130                 135                 140                 
Leu Gly Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
145                 150                 155                 160 
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
                165                 170                 175     
Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
            180                 185                 190         
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
        195                 200                 205             
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
    210                 215                 220                 
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
225                 230                 235                 240 
Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
                245                 250                 255     
Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln
            260                 265                 270         
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
        275                 280                 285             
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
    290                 295                 300                 
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
305                 310                 315                 320 
Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
                325                 330                 335     
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
            340                 345                 350         
Leu Ser Pro Gly Lys
        355         


<210> 167
<211> 1182
<212> DNA
<213> Artificial Sequence

<220> 
<223> LEA29Y-Ig fusion protein coding sequence

<400> 167
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc atcccagttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt ttgtgaatat 180
gcatcccccg gaaaatatac tgaagttaga gtcacagtcc tccgccaggc tgattcacaa 240
gtaacagaag tctgcgctgc aacatatatg atgggcaacg aactcacctt cctcgacgac 300
tcaatttgca caggtacctc ctccggaaat caagttaatc ttactattca aggacttcga 360
gcaatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
gagggtatag gtaatggcac tcagatctac gtcattgacc ccgaaccctg ccctgattct 480
gatcaggagc ccaaatcttc tgacaaaact cacacatccc caccgtcccc agcacctgaa 540
ctcctgggtg gatcgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc 600
tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc 660
aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag 720
gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg 780
ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag 840
aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac cctgccccca 900
tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctat 960
cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc 1020
acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct caccgtggac 1080
aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga ggctctgcac 1140
aaccactaca cgcagaagag cctctccctg tctccgggta aa                    1182

<210> 168
<211> 124
<212> PRT
<213> Artificial Sequence

<220> 
<223> LEA29Y ECD

<400> 168
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Tyr Thr Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr Phe Leu Asp Asp Ser
    50                  55                  60                  
Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
        115                 120                 


<210> 169
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> LEA29Y ECD coding sequence

<400> 169
atgcacgtcg ctcaaccagc cgtcgtactc gcgtcctcta gaggtatcgc ttccttcgtt 60
tgtgaatatg catcccccgg aaaatatact gaagttagag tcacagtcct ccgccaggct 120
gattcacaag taacagaagt ctgcgctgca acatatatga tgggcaacga actcaccttc 180
ctcgacgact caatttgcac aggtacctcc tccggaaatc aagttaatct tactattcaa 240
ggacttcgag caatggatac cggactctat atttgtaaag tcgaactcat gtaccccccc 300
ccttactacg agggtatagg taatggcact cagatctacg tcatcgatcc cgaaccctgc 360
cctgattctg at                                                     372

<210> 170
<211> 476
<212> PRT
<213> Artificial Sequence

<220> 
<223> Predicted WT human CD80-human IgG1 fusion protein

<400> 170
Met Gly His Thr Arg Arg Gln Gly Thr Ser Pro Ser Lys Cys Pro Tyr
 1               5                  10                  15      
Leu Asn Phe Phe Gln Leu Leu Val Leu Ala Gly Leu Ser His Phe Cys
            20                  25                  30          
Ser Gly Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu
        35                  40                  45              
Ser Cys Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile
    50                  55                  60                  
Tyr Trp Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp
65                  70                  75                  80  
Met Asn Ile Trp Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr
                85                  90                  95      
Asn Asn Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly
            100                 105                 110         
Thr Tyr Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg
        115                 120                 125             
Glu His Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr
    130                 135                 140                 
Pro Ser Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile
145                 150                 155                 160 
Ile Cys Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu
                165                 170                 175     
Glu Asn Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ser Gln Asp
            180                 185                 190         
Pro Glu Thr Glu Leu Tyr Ala Val Ser Ser Lys Leu Asp Phe Asn Met
        195                 200                 205             
Thr Thr Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg
    210                 215                 220                 
Val Asn Gln Thr Phe Asn Trp Asn Thr Thr Lys Gln Glu His Phe Pro
225                 230                 235                 240 
Asp Asn Gly Val Thr Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro
                245                 250                 255     
Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe
            260                 265                 270         
Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val
        275                 280                 285             
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe
    290                 295                 300                 
Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro
305                 310                 315                 320 
Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr
                325                 330                 335     
Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val
            340                 345                 350         
Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala
        355                 360                 365             
Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg
    370                 375                 380                 
Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly
385                 390                 395                 400 
Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro
                405                 410                 415     
Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser
            420                 425                 430         
Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln
        435                 440                 445             
Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His
    450                 455                 460                 
Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
465                 470                 475     


<210> 171
<211> 442
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mature WT human CD80-human IgG1 fusion protein 

<400> 171
Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu Ser Cys
 1               5                  10                  15      
Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile Tyr Trp
            20                  25                  30          
Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp Met Asn
        35                  40                  45              
Ile Trp Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr Asn Asn
    50                  55                  60                  
Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly Thr Tyr
65                  70                  75                  80  
Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg Glu His
                85                  90                  95      
Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr Pro Ser
            100                 105                 110         
Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile Ile Cys
        115                 120                 125             
Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu Glu Asn
    130                 135                 140                 
Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ser Gln Asp Pro Glu
145                 150                 155                 160 
Thr Glu Leu Tyr Ala Val Ser Ser Lys Leu Asp Phe Asn Met Thr Thr
                165                 170                 175     
Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg Val Asn
            180                 185                 190         
Gln Thr Phe Asn Trp Asn Thr Thr Lys Gln Glu His Phe Pro Asp Asn
        195                 200                 205             
Gly Val Thr Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
    210                 215                 220                 
Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro
225                 230                 235                 240 
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
                245                 250                 255     
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
            260                 265                 270         
Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
        275                 280                 285             
Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
    290                 295                 300                 
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
305                 310                 315                 320 
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
                325                 330                 335     
Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
            340                 345                 350         
Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
        355                 360                 365             
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
    370                 375                 380                 
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
385                 390                 395                 400 
Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
                405                 410                 415     
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
            420                 425                 430         
Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440         


<210> 172
<211> 1428
<212> DNA
<213> Artificial Sequence

<220> 
<223> WT human CD80-human IgG1 fusion protein coding sequence

<400> 172
atgggccaca cacggaggca gggaacatca ccatccaagt gtccatacct caatttcttt 60
cagctcttgg tgctggctgg tctttctcac ttctgttcag gtgttatcca cgtgaccaag 120
gaagtgaaag aagtggcaac gctgtcctgt ggtcacaatg tttctgttga agagctggca 180
caaactcgca tctactggca aaaggagaag aaaatggtgc tgactatgat gtctggggac 240
atgaatatat ggcccgagta caagaaccgg accatctttg atatcactaa taacctctcc 300
atcgtgatcc tggctctgcg cccatctgac gagggcacat acgagtgtgt tgttctgaag 360
tatgaaaaag acgctttcaa gcgggaacac ctggctgaag tgacgttatc agtcaaagct 420
gacttcccta cacctagtat atctgacttt gaaattccaa cttctaatat tagaaggata 480
atttgctcaa cctctggagg ttttcctgag cctcacctct cctggctgga aaatggagaa 540
gaattaaatg ccatcaacac aacagtttcc caagatcctg aaactgagct ctatgctgtt 600
agcagcaaac tggatttcaa tatgacaacc aaccacagct tcatgtgtct catcaagtat 660
ggacatttaa gagtgaatca gaccttcaac tggaatacaa cgaagcaaga gcattttcct 720
gataacgggg tgaccccaaa gagctgcgac aaaactcaca catgcccacc gtgcccagca 780
cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc 840
atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct 900
gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg 960
cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag 1020
gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc 1080
atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg 1140
cccccatccc gggatgagct gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc 1200
ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac 1260
aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag caagctcacc 1320
gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggct 1380
ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaa              1428

<210> 173
<211> 726
<212> DNA
<213> Artificial Sequence

<220> 
<223> Coding sequence of WT human CD80 signal peptide and WT human CD80 ECD

<400> 173
atgggccaca cacggaggca gggaacatca ccatccaagt gtccatacct caatttcttt 60
cagctcttgg tgctggctgg tctttctcac ttctgttcag gtgttatcca cgtgaccaag 120
gaagtgaaag aagtggcaac gctgtcctgt ggtcacaatg tttctgttga agagctggca 180
caaactcgca tctactggca aaaggagaag aaaatggtgc tgactatgat gtctggggac 240
atgaatatat ggcccgagta caagaaccgg accatctttg atatcactaa taacctctcc 300
atcgtgatcc tggctctgcg cccatctgac gagggcacat acgagtgtgt tgttctgaag 360
tatgaaaaag acgctttcaa gcgggaacac ctggctgaag tgacgttatc agtcaaagct 420
gacttcccta cacctagtat atctgacttt gaaattccaa cttctaatat tagaaggata 480
atttgctcaa cctctggagg ttttcctgag cctcacctct cctggctgga aaatggagaa 540
gaattaaatg ccatcaacac aacagtttcc caagatcctg aaactgagct ctatgctgtt 600
agcagcaaac tggatttcaa tatgacaacc aaccacagct tcatgtgtct catcaagtat 660
ggacatttaa gagtgaatca gaccttcaac tggaatacaa cgaagcaaga gcattttcct 720
gataac                                                            726

<210> 174
<211> 208
<212> PRT
<213> Homo Sapiens

<220> 
<223> WT human CD80 ECD

<400> 174
Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu Ser Cys
 1               5                  10                  15      
Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile Tyr Trp
            20                  25                  30          
Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp Met Asn
        35                  40                  45              
Ile Trp Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr Asn Asn
    50                  55                  60                  
Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly Thr Tyr
65                  70                  75                  80  
Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg Glu His
                85                  90                  95      
Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr Pro Ser
            100                 105                 110         
Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile Ile Cys
        115                 120                 125             
Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu Glu Asn
    130                 135                 140                 
Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ser Gln Asp Pro Glu
145                 150                 155                 160 
Thr Glu Leu Tyr Ala Val Ser Ser Lys Leu Asp Phe Asn Met Thr Thr
                165                 170                 175     
Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg Val Asn
            180                 185                 190         
Gln Thr Phe Asn Trp Asn Thr Thr Lys Gln Glu His Phe Pro Asp Asn
        195                 200                 205             


<210> 175
<211> 329
<212> PRT
<213> Homo Sapiens

<220> 
<223> Predicted full-length WT human CD86

<400> 175
Met Asp Pro Gln Cys Thr Met Gly Leu Ser Asn Ile Leu Phe Val Met
 1               5                  10                  15      
Ala Phe Leu Leu Ser Gly Ala Ala Pro Leu Lys Ile Gln Ala Tyr Phe
            20                  25                  30          
Asn Glu Thr Ala Asp Leu Pro Cys Gln Phe Ala Asn Ser Gln Asn Gln
        35                  40                  45              
Ser Leu Ser Glu Leu Val Val Phe Trp Gln Asp Gln Glu Asn Leu Val
    50                  55                  60                  
Leu Asn Glu Val Tyr Leu Gly Lys Glu Lys Phe Asp Ser Val His Ser
65                  70                  75                  80  
Lys Tyr Met Gly Arg Thr Ser Phe Asp Ser Asp Ser Trp Thr Leu Arg
                85                  90                  95      
Leu His Asn Leu Gln Ile Lys Asp Lys Gly Leu Tyr Gln Cys Ile Ile
            100                 105                 110         
His His Lys Lys Pro Thr Gly Met Ile Arg Ile His Gln Met Asn Ser
        115                 120                 125             
Glu Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Val Pro Ile
    130                 135                 140                 
Ser Asn Ile Thr Glu Asn Val Tyr Ile Asn Leu Thr Cys Ser Ser Ile
145                 150                 155                 160 
His Gly Tyr Pro Glu Pro Lys Lys Met Ser Val Leu Leu Arg Thr Lys
                165                 170                 175     
Asn Ser Thr Ile Glu Tyr Asp Gly Ile Met Gln Lys Ser Gln Asp Asn
            180                 185                 190         
Val Thr Glu Leu Tyr Asp Val Ser Ile Ser Leu Ser Val Ser Phe Pro
        195                 200                 205             
Asp Val Thr Ser Asn Met Thr Ile Phe Cys Ile Leu Glu Thr Asp Lys
    210                 215                 220                 
Thr Arg Leu Leu Ser Ser Pro Phe Ser Ile Glu Leu Glu Asp Pro Gln
225                 230                 235                 240 
Pro Pro Pro Asp His Ile Pro Trp Ile Thr Ala Val Leu Pro Thr Val
                245                 250                 255     
Ile Ile Cys Val Met Val Phe Cys Leu Ile Leu Trp Lys Trp Lys Lys
            260                 265                 270         
Lys Lys Arg Pro Arg Asn Ser Tyr Lys Cys Gly Thr Asn Thr Met Glu
        275                 280                 285             
Arg Glu Glu Ser Glu Gln Thr Lys Lys Arg Glu Lys Ile His Ile Pro
    290                 295                 300                 
Glu Arg Ser Asp Glu Ala Gln Arg Val Phe Lys Ser Ser Lys Thr Ser
305                 310                 315                 320 
Ser Cys Asp Lys Ser Asp Thr Cys Phe
                325                 


<210> 176
<211> 987
<212> DNA
<213> Artificial Sequence

<220> 
<223> full-length WT human CD86 coding sequence

<400> 176
atggatcccc agtgcactat gggactgagt aacattctct ttgtgatggc cttcctgctc 60
tctggtgctg ctcctctgaa gattcaagct tatttcaatg agactgcaga cctgccatgc 120
caatttgcaa actctcaaaa ccaaagcctg agtgagctag tagtattttg gcaggaccag 180
gaaaacttgg ttctgaatga ggtatactta ggcaaagaga aatttgacag tgttcattcc 240
aagtatatgg gccgcacaag ttttgattcg gacagttgga ccctgagact tcacaatctt 300
cagatcaagg acaagggctt gtatcaatgt atcatccatc acaaaaagcc cacaggaatg 360
attcgcatcc accagatgaa ttctgaactg tcagtgcttg ctaacttcag tcaacctgaa 420
atagtaccaa tttctaatat aacagaaaat gtgtacataa atttgacctg ctcatctata 480
cacggttacc cagaacctaa gaagatgagt gttttgctaa gaaccaagaa ttcaactatc 540
gagtatgatg gtattatgca gaaatctcaa gataatgtca cagaactgta cgacgtttcc 600
atcagcttgt ctgtttcatt ccctgatgtt acgagcaata tgaccatctt ctgtattctg 660
gaaactgaca agacgcggct tttatcttca cctttctcta tagagcttga ggaccctcag 720
cctcccccag accacattcc ttggattaca gctgtacttc caacagttat tatatgtgtg 780
atggttttct gtctaattct atggaaatgg aagaagaaga agcggcctcg caactcttat 840
aaatgtggaa ccaacacaat ggagagggaa gagagtgaac agaccaagaa aagagaaaaa 900
atccatatac ctgaaagatc tgatgaagcc cagcgtgttt ttaaaagttc gaagacatct 960
tcatgcgaca aaagtgatac atgtttt                                     987

<210> 177
<211> 477
<212> PRT
<213> Artificial Sequence

<220> 
<223> Predicted WT human CD86-human IgG1 fusion protein 

<400> 177
Met Asp Pro Gln Cys Thr Met Gly Leu Ser Asn Ile Leu Phe Val Met
 1               5                  10                  15      
Ala Phe Leu Leu Ser Gly Ala Ala Pro Leu Lys Ile Gln Ala Tyr Phe
            20                  25                  30          
Asn Glu Thr Ala Asp Leu Pro Cys Gln Phe Ala Asn Ser Gln Asn Gln
        35                  40                  45              
Ser Leu Ser Glu Leu Val Val Phe Trp Gln Asp Gln Glu Asn Leu Val
    50                  55                  60                  
Leu Asn Glu Val Tyr Leu Gly Lys Glu Lys Phe Asp Ser Val His Ser
65                  70                  75                  80  
Lys Tyr Met Gly Arg Thr Ser Phe Asp Ser Asp Ser Trp Thr Leu Arg
                85                  90                  95      
Leu His Asn Leu Gln Ile Lys Asp Lys Gly Leu Tyr Gln Cys Ile Ile
            100                 105                 110         
His His Lys Lys Pro Thr Gly Met Ile Arg Ile His Gln Met Asn Ser
        115                 120                 125             
Glu Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Val Pro Ile
    130                 135                 140                 
Ser Asn Ile Thr Glu Asn Val Tyr Ile Asn Leu Thr Cys Ser Ser Ile
145                 150                 155                 160 
His Gly Tyr Pro Glu Pro Lys Lys Met Ser Val Leu Leu Arg Thr Lys
                165                 170                 175     
Asn Ser Thr Ile Glu Tyr Asp Gly Ile Met Gln Lys Ser Gln Asp Asn
            180                 185                 190         
Val Thr Glu Leu Tyr Asp Val Ser Ile Ser Leu Ser Val Ser Phe Pro
        195                 200                 205             
Asp Val Thr Ser Asn Met Thr Ile Phe Cys Ile Leu Glu Thr Asp Lys
    210                 215                 220                 
Thr Arg Leu Leu Ser Ser Pro Phe Ser Ile Glu Leu Glu Asp Pro Gln
225                 230                 235                 240 
Pro Pro Pro Gly Val Thr Pro Lys Ser Cys Asp Lys Thr His Thr Cys
                245                 250                 255     
Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu
            260                 265                 270         
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu
        275                 280                 285             
Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys
    290                 295                 300                 
Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys
305                 310                 315                 320 
Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu
                325                 330                 335     
Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys
            340                 345                 350         
Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys
        355                 360                 365             
Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser
    370                 375                 380                 
Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
385                 390                 395                 400 
Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln
                405                 410                 415     
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly
            420                 425                 430         
Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln
        435                 440                 445             
Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn
    450                 455                 460                 
His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
465                 470                 475         


<210> 178
<211> 454
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mature WT human CD86-human IgG1 fusion protein 

<400> 178
Ala Pro Leu Lys Ile Gln Ala Tyr Phe Asn Glu Thr Ala Asp Leu Pro
 1               5                  10                  15      
Cys Gln Phe Ala Asn Ser Gln Asn Gln Ser Leu Ser Glu Leu Val Val
            20                  25                  30          
Phe Trp Gln Asp Gln Glu Asn Leu Val Leu Asn Glu Val Tyr Leu Gly
        35                  40                  45              
Lys Glu Lys Phe Asp Ser Val His Ser Lys Tyr Met Gly Arg Thr Ser
    50                  55                  60                  
Phe Asp Ser Asp Ser Trp Thr Leu Arg Leu His Asn Leu Gln Ile Lys
65                  70                  75                  80  
Asp Lys Gly Leu Tyr Gln Cys Ile Ile His His Lys Lys Pro Thr Gly
                85                  90                  95      
Met Ile Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala Asn
            100                 105                 110         
Phe Ser Gln Pro Glu Ile Val Pro Ile Ser Asn Ile Thr Glu Asn Val
        115                 120                 125             
Tyr Ile Asn Leu Thr Cys Ser Ser Ile His Gly Tyr Pro Glu Pro Lys
    130                 135                 140                 
Lys Met Ser Val Leu Leu Arg Thr Lys Asn Ser Thr Ile Glu Tyr Asp
145                 150                 155                 160 
Gly Ile Met Gln Lys Ser Gln Asp Asn Val Thr Glu Leu Tyr Asp Val
                165                 170                 175     
Ser Ile Ser Leu Ser Val Ser Phe Pro Asp Val Thr Ser Asn Met Thr
            180                 185                 190         
Ile Phe Cys Ile Leu Glu Thr Asp Lys Thr Arg Leu Leu Ser Ser Pro
        195                 200                 205             
Phe Ser Ile Glu Leu Glu Asp Pro Gln Pro Pro Pro Gly Val Thr Pro
    210                 215                 220                 
Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu
225                 230                 235                 240 
Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
                245                 250                 255     
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
            260                 265                 270         
Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly
        275                 280                 285             
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
    290                 295                 300                 
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp
305                 310                 315                 320 
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro
                325                 330                 335     
Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
            340                 345                 350         
Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn
        355                 360                 365             
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile
    370                 375                 380                 
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr
385                 390                 395                 400 
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys
                405                 410                 415     
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys
            420                 425                 430         
Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
        435                 440                 445             
Ser Leu Ser Pro Gly Lys
    450                 


<210> 179
<211> 1431
<212> DNA
<213> Artificial Sequence

<220> 
<223> WT human CD86-human IgG1 fusion protein coding sequence

<400> 179
atggatcccc agtgcactat gggactgagt aacattctct ttgtgatggc cttcctgctc 60
tctggtgctg ctcctctgaa gattcaagct tatttcaatg agactgcaga cctgccatgc 120
caatttgcaa actctcaaaa ccaaagcctg agtgagctag tagtattttg gcaggaccag 180
gaaaacttgg ttctgaatga ggtatactta ggcaaagaga aatttgacag tgttcattcc 240
aagtatatgg gccgcacaag ttttgattcg gacagttgga ccctgagact tcacaatctt 300
cagatcaagg acaagggctt gtatcaatgt atcatccatc acaaaaagcc cacaggaatg 360
attcgcatcc accagatgaa ttctgaactg tcagtgcttg ctaacttcag tcaacctgaa 420
atagtaccaa tttctaatat aacagaaaat gtgtacataa atttgacctg ctcatctata 480
cacggctacc cagaacctaa gaagatgagt gttttgctaa gaaccaagaa ttcaactatc 540
gagtatgatg gtattatgca gaaatctcaa gataatgtca cagaactgta cgacgtttcc 600
atcagcttgt ctgtttcatt ccctgatgtt acgagcaata tgaccatctt ctgtattctg 660
gaaactgaca agacgcggct tttatcttca cctttctcta tagagcttga ggaccctcag 720
cctcccccag gggtgacccc aaagagctgc gacaaaactc acacatgccc accgtgccca 780
gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc caaggacacc 840
ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac 900
cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag 960
ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac 1020
caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc 1080
cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca ggtgtacacc 1140
ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg cctggtcaaa 1200
ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac 1260
tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc 1320
accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt gatgcatgag 1380
gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa a          1431

<210> 180
<211> 220
<212> PRT
<213> Homo Sapiens

<220> 
<223> WT human CD86 ECD 

<400> 180
Ala Pro Leu Lys Ile Gln Ala Tyr Phe Asn Glu Thr Ala Asp Leu Pro
 1               5                  10                  15      
Cys Gln Phe Ala Asn Ser Gln Asn Gln Ser Leu Ser Glu Leu Val Val
            20                  25                  30          
Phe Trp Gln Asp Gln Glu Asn Leu Val Leu Asn Glu Val Tyr Leu Gly
        35                  40                  45              
Lys Glu Lys Phe Asp Ser Val His Ser Lys Tyr Met Gly Arg Thr Ser
    50                  55                  60                  
Phe Asp Ser Asp Ser Trp Thr Leu Arg Leu His Asn Leu Gln Ile Lys
65                  70                  75                  80  
Asp Lys Gly Leu Tyr Gln Cys Ile Ile His His Lys Lys Pro Thr Gly
                85                  90                  95      
Met Ile Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala Asn
            100                 105                 110         
Phe Ser Gln Pro Glu Ile Val Pro Ile Ser Asn Ile Thr Glu Asn Val
        115                 120                 125             
Tyr Ile Asn Leu Thr Cys Ser Ser Ile His Gly Tyr Pro Glu Pro Lys
    130                 135                 140                 
Lys Met Ser Val Leu Leu Arg Thr Lys Asn Ser Thr Ile Glu Tyr Asp
145                 150                 155                 160 
Gly Ile Met Gln Lys Ser Gln Asp Asn Val Thr Glu Leu Tyr Asp Val
                165                 170                 175     
Ser Ile Ser Leu Ser Val Ser Phe Pro Asp Val Thr Ser Asn Met Thr
            180                 185                 190         
Ile Phe Cys Ile Leu Glu Thr Asp Lys Thr Arg Leu Leu Ser Ser Pro
        195                 200                 205             
Phe Ser Ile Glu Leu Glu Asp Pro Gln Pro Pro Pro
    210                 215                 220 


<210> 181
<211> 105
<212> DNA
<213> Artificial Sequence

<220> 
<223> WT human CTLA-4 signal peptide coding sequence

<400> 181
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgt                 105

<210> 182
<211> 35
<212> PRT
<213> Homo Sapiens

<220> 
<223> WT human CTLA-4 signal peptide

<400> 182
Met Ala Cys Leu Gly Phe Gln Arg His Lys Ala Gln Leu Asn Leu Ala
 1               5                  10                  15      
Thr Arg Thr Trp Pro Cys Thr Leu Leu Phe Phe Leu Leu Phe Ile Pro
            20                  25                  30          
Val Phe Cys
        35  


<210> 183
<211> 684
<212> DNA
<213> Artificial Sequence

<220> 
<223>  Coding sequence of human IgG2 Fc polypeptide (with C-terminal lysine) 

<400> 183
gagcgcaaat gttgtgtcga gtgcccaccg tgcccagcac cacctgtggc aggaccgtca 60
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 120
acgtgcgtgg tggtggacgt gagccacgaa gaccccgagg tccagttcaa ctggtacgtg 180
gacggcgtgg aggtgcataa tgccaagaca aagccacggg aggagcagtt caacagcacg 240
ttccgtgtgg tcagcgtcct caccgttgtg caccaggact ggctgaacgg caaggagtac 300
aagtgcaagg tctccaacaa aggcctccca gcccccatcg agaaaaccat ctccaaaacc 360
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc 420
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct accccagcga catcgccgtg 480
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacacctcc catgctggac 540
tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag 600
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 660
agcctctccc tgtctccggg taaa                                        684

<210> 184
<211> 228
<212> PRT
<213> Homo Sapiens

<220> 
<223> Human IgG2 Fc polypeptide (with C-terminal lysine)

<400> 184
Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val
 1               5                  10                  15      
Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu
            20                  25                  30          
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser
        35                  40                  45              
His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu
    50                  55                  60                  
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr
65                  70                  75                  80  
Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn
                85                  90                  95      
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro
            100                 105                 110         
Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln
        115                 120                 125             
Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val
    130                 135                 140                 
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val
145                 150                 155                 160 
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
                165                 170                 175     
Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
            180                 185                 190         
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val
        195                 200                 205             
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
    210                 215                 220                 
Ser Pro Gly Lys
225             


<210> 185
<211> 231
<212> PRT
<213> Homo Sapiens

<220> 
<223> Human IgG1 Fc polypeptide

<400> 185
Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro
 1               5                  10                  15      
Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys
            20                  25                  30          
Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val
        35                  40                  45              
Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp
    50                  55                  60                  
Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr
65                  70                  75                  80  
Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
                85                  90                  95      
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
            100                 105                 110         
Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg
        115                 120                 125             
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys
    130                 135                 140                 
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
145                 150                 155                 160 
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
                165                 170                 175     
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
            180                 185                 190         
Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser
        195                 200                 205             
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
    210                 215                 220                 
Leu Ser Leu Ser Pro Gly Lys
225                 230     


<210> 186
<211> 232
<212> PRT
<213> Artificial Sequence

<220> 
<223> Mutant IgG1 Fc polypeptide

<400> 186
Glu Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala
 1               5                  10                  15      
Pro Glu Leu Leu Gly Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro
            20                  25                  30          
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val
        35                  40                  45              
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val
    50                  55                  60                  
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln
65                  70                  75                  80  
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln
                85                  90                  95      
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala
            100                 105                 110         
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro
        115                 120                 125             
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr
    130                 135                 140                 
Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser
145                 150                 155                 160 
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr
                165                 170                 175     
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr
            180                 185                 190         
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe
        195                 200                 205             
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys
    210                 215                 220                 
Ser Leu Ser Leu Ser Pro Gly Lys
225                 230         


<210> 187
<211> 59
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 187
ctattgctac ggccgctatg gccmtkcacg tcgctcaacc agccgtcgta ctcgcgtcc  59

<210> 188
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 188
gtgatggtga tggtgtgcgg ccgcatcaga                                  30

<210> 189
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 189
aagctgtcac cggtggatcg atcccgaacc ctgccctgat tctgatgagc gcaaatgttg 60
tgtcgagtgc ccaccgt                                                77

<210> 190
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 190
cagaattcat tatttacccg gagacaggga gaggctcttc tg                    42

<210> 191
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 191
ggaataccgg ttttttgtaa agccatgcac gtcgctcaac cagccgtcgt actc       54

<210> 192
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 192
ggcactcaga tctacgtcat cgatcccgaa                                  30

<210> 193
<211> 372
<212> DNA
<213> Artificial Sequence

<220> 
<223> human CTLA-4 ECD coding sequence

<400> 193
atgcacgtgg cccagcctgc tgtggtactg gccagcagcc gaggcatcgc cagctttgtg 60
tgtgagtatg catctccagg caaagccact gaggtccggg tgacagtgct tcggcaggct 120
gacagccagg tgactgaagt ctgtgcggca acctacatga tggggaatga gttgaccttc 180
ctagatgatt ccatctgcac gggcacctcc agtggaaatc aagtgaacct cactatccaa 240
ggactgaggg ccatggacac gggactctac atctgcaagg tggagctcat gtacccaccg 300
ccatactacc tgggcatagg caacggaacc cagatttatg taattgatcc agaaccgtgc 360
ccagattctg ac                                                     372

<210> 194
<211> 672
<212> DNA
<213> Artificial Sequence

<220> 
<223> full-length human CTLA-4 coding sequence

<400> 194
atggcttgcc ttggatttca gcggcacaag gctcagctga acctggctac caggacctgg 60
ccctgcactc tcctgttttt tcttctcttc atccctgtct tctgcaaagc aatgcacgtg 120
gcccagcctg ctgtggtact ggccagcagc cgaggcatcg ccagctttgt gtgtgagtat 180
gcatctccag gcaaagccac tgaggtccgg gtgacagtgc ttcggcaggc tgacagccag 240
gtgactgaag tctgtgcggc aacctacatg atggggaatg agttgacctt cctagatgat 300
tccatctgca cgggcacctc cagtggaaat caagtgaacc tcactatcca aggactgagg 360
gccatggaca cgggactcta catctgcaag gtggagctca tgtacccacc gccatactac 420
ctgggcatag gcaacggaac ccagatttat gtaattgatc cagaaccgtg cccagattct 480
gacttcctcc tctggatcct tgcagcagtt agttcggggt tgttttttta tagctttctc 540
ctcacagctg tttctttgag caaaatgcta aagaaaagaa gccctcttac aacaggggtc 600
tatgtgaaaa tgcccccaac agagccagaa tgtgaaaagc aatttcagcc ttattttatt 660
cccatcaatt ga                                                     672

<210> 195
<211> 288
<212> PRT
<213> Homo Sapiens

<220> 
<223> Predicted full-length human CD80 

<400> 195
Met Gly His Thr Arg Arg Gln Gly Thr Ser Pro Ser Lys Cys Pro Tyr
 1               5                  10                  15      
Leu Asn Phe Phe Gln Leu Leu Val Leu Ala Gly Leu Ser His Phe Cys
            20                  25                  30          
Ser Gly Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu
        35                  40                  45              
Ser Cys Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile
    50                  55                  60                  
Tyr Trp Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp
65                  70                  75                  80  
Met Asn Ile Trp Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr
                85                  90                  95      
Asn Asn Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly
            100                 105                 110         
Thr Tyr Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg
        115                 120                 125             
Glu His Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr
    130                 135                 140                 
Pro Ser Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile
145                 150                 155                 160 
Ile Cys Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu
                165                 170                 175     
Glu Asn Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ser Gln Asp
            180                 185                 190         
Pro Glu Thr Glu Leu Tyr Ala Val Ser Ser Lys Leu Asp Phe Asn Met
        195                 200                 205             
Thr Thr Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg
    210                 215                 220                 
Val Asn Gln Thr Phe Asn Trp Asn Thr Thr Lys Gln Glu His Phe Pro
225                 230                 235                 240 
Asp Asn Leu Leu Pro Ser Trp Ala Ile Thr Leu Ile Ser Val Asn Gly
                245                 250                 255     
Ile Phe Val Ile Cys Cys Leu Thr Tyr Cys Phe Ala Pro Arg Cys Arg
            260                 265                 270         
Glu Arg Arg Arg Asn Glu Arg Leu Arg Arg Glu Ser Val Arg Pro Val
        275                 280                 285             


<210> 196
<211> 867
<212> DNA
<213> Artificial Sequence

<220> 
<223> full-length human CD80 coding sequence

<400> 196
atgggccaca cacggaggca gggaacatca ccatccaagt gtccatacct caatttcttt 60
cagctcttgg tgctggctgg tctttctcac ttctgttcag gtgttatcca cgtgaccaag 120
gaagtgaaag aagtggcaac gctgtcctgt ggtcacaatg tttctgttga agagctggca 180
caaactcgca tctactggca aaaggagaag aaaatggtgc tgactatgat gtctggggac 240
atgaatatat ggcccgagta caagaaccgg accatctttg atatcactaa taacctctcc 300
attgtgatcc tggctctgcg cccatctgac gagggcacat acgagtgtgt tgttctgaag 360
tatgaaaaag acgctttcaa gcgggaacac ctggctgaag tgacgttatc agtcaaagct 420
gacttcccta cacctagtat atctgacttt gaaattccaa cttctaatat tagaaggata 480
atttgctcaa cctctggagg ttttccagag cctcacctct cctggttgga aaatggagaa 540
gaattaaatg ccatcaacac aacagtttcc caagatcctg aaactgagct ctatgctgtt 600
agcagcaaac tggatttcaa tatgacaacc aaccacagct tcatgtgtct catcaagtat 660
ggacatttaa gagtgaatca gaccttcaac tggaatacaa ccaagcaaga gcattttcct 720
gataacctgc tcccatcctg ggccattacc ttaatctcag taaatggaat ttttgtgata 780
tgctgcctga cctactgctt tgccccaaga tgcagagaga gaaggaggaa tgagagattg 840
agaagggaaa gtgtacgccc tgtataa                                     867

<210> 197
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-54-IgG2 fusion protein (with C-terminal lysine)

<400> 197
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 198
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-56-IgG2 fusion protein (with C-terminal lysine)

<400> 198
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 199
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-69-IgG2 fusion protein (with C-terminal lysine)

<400> 199
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 200
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-75-IgG2 fusion protein (with C-terminal lysine)

<400> 200
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 201
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-54-IgG2 fusion protein coding sequence

<400> 201
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gcgtcacccg gcaaagccaa cgaagttaga gtcacagtgc tccgccaagc cgatagtcaa 240
gttacagaag tctgcgctat gacatacatg aaggaaaatg aactaacctt ccttgacgat 300
cccatctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
ttgggtattg gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 202
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-56-IgG2 fusion protein coding sequence

<400> 202
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gcgtcacccg gcaaagccaa cgaagttaga gtcacagtgc tccgccaagc cgatagtcaa 240
gttacagaag tctgcgctat gacatacatg aaggaaaatg aactaacctt ccttgacgat 300
cccagctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
ttgggtattg gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 203
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-69-IgG2 fusion protein coding sequence

<400> 203
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gagtcacccg gcaaagccaa cgaaattaga gtcacagtgc tccgccaagc cgatagtcaa 240
gttacagaag tctgcgctat gacatacatg aagggagatg aactaacctt ccttgacgat 300
cccagctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
gagggtatag gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 204
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-75-IgG2 fusion protein coding sequence

<400> 204
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gagtcacccg gcaaagccaa cgaagttaga gtcacagtgc tccgccaagc cgatagtcaa 240
gttacagaag tctgcgctat gacatacatg aaggaaaatg aactaacctt ccttgacgat 300
cccagctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
ttgggtattg gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 205
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-12-IgG2 fusion protein (without C-terminal lysine)

<400> 205
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 206
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-14-IgG2 fusion protein (without C-terminal lysine)

<400> 206
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Ala Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 207
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-17-IgG2 fusion protein (without C-terminal lysine)

<400> 207
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 208
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-20-IgG2 fusion protein (without C-terminal lysine)

<400> 208
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 209
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-27-IgG2 fusion protein (without C-terminal lysine)

<400> 209
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 210
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-29-IgG2 fusion protein (without C-terminal lysine)

<400> 210
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 211
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-54-IgG2 fusion protein (without C-terminal lysine)

<400> 211
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 212
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-56-IgG2 fusion protein (without C-terminal lysine)

<400> 212
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 213
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-69-IgG2 fusion protein (without C-terminal lysine)

<400> 213
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 214
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-75-IgG2 fusion protein (without C-terminal lysine)

<400> 214
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Glu Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asn Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 215
<211> 111
<212> DNA
<213> Artificial Sequence

<220> 
<223> Coding sequence of WT hCTLA-4 signal peptide sequence comprising amino acid residues 1-37 of full-length hCTLA-4 protein

<400> 215
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc c          111

<210> 216
<211> 37
<212> PRT
<213> Homo sapiens

<220> 
<223> WT hCTLA-4 signal peptide sequence comprising amino acid residues 1-37 of full-length hCTLA-4 protein

<400> 216
Met Ala Cys Leu Gly Phe Gln Arg His Lys Ala Gln Leu Asn Leu Ala
 1               5                  10                  15      
Thr Arg Thr Trp Pro Cys Thr Leu Leu Phe Phe Leu Leu Phe Ile Pro
            20                  25                  30          
Val Phe Cys Lys Ala
        35          


<210> 217
<211> 684
<212> DNA
<213> Artificial Sequence

<220> 
<223> Coding sequence of human IgG2 Fc polypeptide 

<400> 217
gagcgcaaat gttgtgtcga gtgcccaccg tgcccagcac cacctgtggc aggaccgtca 60
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 120
acgtgcgtgg tggtggacgt gagccacgaa gaccccgagg tccagttcaa ctggtacgtg 180
gacggcgtgg aggtgcataa tgccaagaca aagccacggg aggagcagtt caacagcacg 240
ttccgtgtgg tcagcgtcct caccgttgtg caccaggact ggctgaacgg caaggagtac 300
aagtgcaagg tctccaacaa aggcctccca gcccccatcg agaaaaccat ctccaaaacc 360
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc 420
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct accccagcga catcgccgtg 480
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacacctcc catgctggac 540
tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag 600
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 660
agcctctccc tgtctccggg taaa                                        684

<210> 218
<211> 227
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human IgG2 Fc polypeptide (without C-terminal lysine)

<400> 218
Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val
 1               5                  10                  15      
Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu
            20                  25                  30          
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser
        35                  40                  45              
His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu
    50                  55                  60                  
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr
65                  70                  75                  80  
Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn
                85                  90                  95      
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro
            100                 105                 110         
Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln
        115                 120                 125             
Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val
    130                 135                 140                 
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val
145                 150                 155                 160 
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
                165                 170                 175     
Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
            180                 185                 190         
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val
        195                 200                 205             
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
    210                 215                 220                 
Ser Pro Gly
225         


<210> 219
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-71-IgG2 fusion protein (without C-terminal lysine)

<400> 219
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 220
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-71-IgG2 fusion protein (with C-terminal lysine)

<400> 220
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Ile
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Gly Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ser Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Glu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 221
<211> 351
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-76-IgG2 fusion protein (without C-terminal lysine)

<400> 221
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
            340                 345                 350     


<210> 222
<211> 352
<212> PRT
<213> Artificial Sequence

<220> 
<223> D3-76-IgG2 fusion protein (with C-terminal lysine)

<400> 222
Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile
 1               5                  10                  15      
Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Asn Glu Val
            20                  25                  30          
Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys
        35                  40                  45              
Ala Met Thr Tyr Met Lys Glu Asp Glu Leu Thr Phe Leu Asp Asp Pro
    50                  55                  60                  
Ile Cys Thr Gly Thr Phe Ser Gly Asn Gln Val Asn Leu Thr Ile Gln
65                  70                  75                  80  
Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu
                85                  90                  95      
Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile
            100                 105                 110         
Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Glu Arg Lys Cys
        115                 120                 125             
Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser
    130                 135                 140                 
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
145                 150                 155                 160 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
                165                 170                 175     
Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
            180                 185                 190         
Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val
        195                 200                 205             
Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
    210                 215                 220                 
Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr
225                 230                 235                 240 
Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
                245                 250                 255     
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
            260                 265                 270         
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
        275                 280                 285             
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp
    290                 295                 300                 
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
305                 310                 315                 320 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
                325                 330                 335     
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            340                 345                 350         


<210> 223
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-71-IgG2 fusion protein coding sequence

<400> 223
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gcgtcacccg gcaaagccaa cgaaattaga gtcacagtgc tccgccaagc cgatagtcaa 240
gttacagaag tctgcgctat gacatacatg aagggagatg aactaacctt ccttgacgat 300
cccagctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
gagggtatag gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 224
<211> 1170
<212> DNA
<213> Artificial Sequence

<220> 
<223> D3-76-IgG2 fusion protein coding sequence

<400> 224
atggcttgtc ttggatttca acgccacaaa gctcaactca atctcgctac ccgaacatgg 60
ccttgtactc ttctcttttt tctcctcttc ataccggttt tttgtaaagc catgcacgtc 120
gctcaaccag ccgtcgtact cgcgtcctct agaggtatcg cttccttcgt gtgtgaatat 180
gcgtcacccg gcaaagccaa cgaagttaga gtcacagtgc tccgccaagc cgatagtcaa 240
gttacagaag tctgcgctat gacatacatg aaggaagatg aactaacctt ccttgacgat 300
cccatctgca caggtacctt ttccggaaat caagttaatc ttactattca aggacttcga 360
gccatggata ccggactcta tatttgtaaa gtcgaactca tgtacccccc cccttactac 420
ttgggtattg gtaatggcac tcagatctac gtcatcgatc ccgaaccctg ccctgattct 480
gatgagcgca aatgttgtgt cgagtgccca ccgtgcccag caccacctgt ggcaggaccg 540
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 600
gtcacgtgcg tggtggtgga cgtgagccac gaagaccccg aggtccagtt caactggtac 660
gtggacggcg tggaggtgca taatgccaag acaaagccac gggaggagca gttcaacagc 720
acgttccgtg tggtcagcgt cctcaccgtt gtgcaccagg actggctgaa cggcaaggag 780
tacaagtgca aggtctccaa caaaggcctc ccagccccca tcgagaaaac catctccaaa 840
accaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 900
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc 960
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacacc tcccatgctg 1020
gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag 1080
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1140
aagagcctct ccctgtctcc gggtaaataa                                  1170

<210> 225
<211> 485
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human CD86-murine Ig fusion protein

<400> 225
Met Asp Pro Gln Cys Thr Met Gly Leu Ser Asn Ile Leu Phe Val Met
 1               5                  10                  15      
Ala Phe Leu Leu Ser Gly Ala Ala Pro Leu Lys Ile Gln Ala Tyr Phe
            20                  25                  30          
Asn Glu Thr Ala Asp Leu Pro Cys Gln Phe Ala Asn Ser Gln Asn Gln
        35                  40                  45              
Ser Leu Ser Glu Leu Val Val Phe Trp Gln Asp Gln Glu Asn Leu Val
    50                  55                  60                  
Leu Asn Glu Val Tyr Leu Gly Lys Glu Lys Phe Asp Ser Val His Ser
65                  70                  75                  80  
Lys Tyr Met Gly Arg Thr Ser Phe Asp Ser Asp Ser Trp Thr Leu Arg
                85                  90                  95      
Leu His Asn Leu Gln Ile Lys Asp Lys Gly Leu Tyr Gln Cys Ile Ile
            100                 105                 110         
His His Lys Lys Pro Thr Gly Met Ile Arg Ile His Gln Met Asn Ser
        115                 120                 125             
Glu Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Val Pro Ile
    130                 135                 140                 
Ser Asn Ile Thr Glu Asn Val Tyr Ile Asn Leu Thr Cys Ser Ser Ile
145                 150                 155                 160 
His Gly Tyr Pro Glu Pro Lys Lys Met Ser Val Leu Leu Arg Thr Lys
                165                 170                 175     
Asn Ser Thr Ile Glu Tyr Asp Gly Ile Met Gln Lys Ser Gln Asp Asn
            180                 185                 190         
Val Thr Glu Leu Tyr Asp Val Ser Ile Ser Leu Ser Val Ser Phe Pro
        195                 200                 205             
Asp Val Thr Ser Asn Met Thr Ile Phe Cys Ile Leu Glu Thr Asp Lys
    210                 215                 220                 
Thr Arg Leu Leu Ser Ser Pro Phe Ser Ile Glu Leu Glu Asp Pro Gln
225                 230                 235                 240 
Pro Pro Pro Gly Val Thr Pro Arg Val Pro Ile Thr Gln Asn Pro Cys
                245                 250                 255     
Pro Pro Leu Lys Glu Cys Pro Pro Cys Ala Ala Pro Asp Leu Leu Gly
            260                 265                 270         
Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met
        275                 280                 285             
Ile Ser Leu Ser Pro Met Val Thr Cys Val Val Val Asp Val Ser Glu
    290                 295                 300                 
Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val
305                 310                 315                 320 
His Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu
                325                 330                 335     
Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly
            340                 345                 350         
Lys Glu Phe Lys Cys Lys Val Asn Asn Arg Ala Leu Pro Ser Pro Ile
        355                 360                 365             
Glu Lys Thr Ile Ser Lys Pro Arg Gly Pro Val Arg Ala Pro Gln Val
    370                 375                 380                 
Tyr Val Leu Pro Pro Pro Ala Glu Glu Met Thr Lys Lys Glu Phe Ser
385                 390                 395                 400 
Leu Thr Cys Met Ile Thr Gly Phe Leu Pro Ala Glu Ile Ala Val Asp
                405                 410                 415     
Trp Thr Ser Asn Gly Arg Thr Glu Gln Asn Tyr Lys Asn Thr Ala Thr
            420                 425                 430         
Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val
        435                 440                 445             
Gln Lys Ser Thr Trp Glu Arg Gly Ser Leu Phe Ala Cys Ser Val Val
    450                 455                 460                 
His Glu Gly Leu His Asn His Leu Thr Thr Lys Thr Ile Ser Arg Ser
465                 470                 475                 480 
Leu Gly Lys Ser Gly
                485 


<210> 226
<211> 484
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human CD80-murine Ig fusion protein

<400> 226
Met Gly His Thr Arg Arg Gln Gly Thr Ser Pro Ser Lys Cys Pro Tyr
 1               5                  10                  15      
Leu Asn Phe Phe Gln Leu Leu Val Leu Ala Gly Leu Ser His Phe Cys
            20                  25                  30          
Ser Gly Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu
        35                  40                  45              
Ser Cys Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile
    50                  55                  60                  
Tyr Trp Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp
65                  70                  75                  80  
Met Asn Ile Trp Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr
                85                  90                  95      
Asn Asn Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly
            100                 105                 110         
Thr Tyr Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg
        115                 120                 125             
Glu His Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr
    130                 135                 140                 
Pro Ser Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile
145                 150                 155                 160 
Ile Cys Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu
                165                 170                 175     
Glu Asn Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ser Gln Asp
            180                 185                 190         
Pro Glu Thr Glu Leu Tyr Ala Val Ser Ser Lys Leu Asp Phe Asn Met
        195                 200                 205             
Thr Thr Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg
    210                 215                 220                 
Val Asn Gln Thr Phe Asn Trp Asn Thr Thr Lys Gln Glu His Phe Pro
225                 230                 235                 240 
Asp Asn Gly Val Thr Pro Arg Val Pro Ile Thr Gln Asn Pro Cys Pro
                245                 250                 255     
Pro Leu Lys Glu Cys Pro Pro Cys Ala Ala Pro Asp Leu Leu Gly Gly
            260                 265                 270         
Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile
        275                 280                 285             
Ser Leu Ser Pro Met Val Thr Cys Val Val Val Asp Val Ser Glu Asp
    290                 295                 300                 
Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His
305                 310                 315                 320 
Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg
                325                 330                 335     
Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys
            340                 345                 350         
Glu Phe Lys Cys Lys Val Asn Asn Arg Ala Leu Pro Ser Pro Ile Glu
        355                 360                 365             
Lys Thr Ile Ser Lys Pro Arg Gly Pro Val Arg Ala Pro Gln Val Tyr
    370                 375                 380                 
Val Leu Pro Pro Pro Ala Glu Glu Met Thr Lys Lys Glu Phe Ser Leu
385                 390                 395                 400 
Thr Cys Met Ile Thr Gly Phe Leu Pro Ala Glu Ile Ala Val Asp Trp
                405                 410                 415     
Thr Ser Asn Gly Arg Thr Glu Gln Asn Tyr Lys Asn Thr Ala Thr Val
            420                 425                 430         
Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Gln
        435                 440                 445             
Lys Ser Thr Trp Glu Arg Gly Ser Leu Phe Ala Cys Ser Val Val His
    450                 455                 460                 
Glu Gly Leu His Asn His Leu Thr Thr Lys Thr Ile Ser Arg Ser Leu
465                 470                 475                 480 
Gly Lys Ser Gly
                


<210> 227
<211> 1458
<212> DNA
<213> Artificial Sequence

<220> 
<223> Human CD86-murine Ig fusion protein coding sequence

<400> 227
atggatcccc agtgcactat gggactgagt aacattctct ttgtgatggc cttcctgctc 60
tctggtgctg ctcctctgaa gattcaagct tatttcaatg agactgcaga cctgccatgc 120
caatttgcaa actctcaaaa ccaaagcctg agtgagctag tagtattttg gcaggaccag 180
gaaaacttgg ttctgaatga ggtatactta ggcaaagaga aatttgacag tgttcattcc 240
aagtatatgg gccgcacaag ttttgattcg gacagttgga ccctgagact tcacaatctt 300
cagatcaagg acaagggctt gtatcaatgt atcatccatc acaaaaagcc cacaggaatg 360
attcgcatcc accagatgaa ttctgaactg tcagtgcttg ctaacttcag tcaacctgaa 420
atagtaccaa tttctaatat aacagaaaat gtgtacataa atttgacctg ctcatctata 480
cacggctacc cagaacctaa gaagatgagt gttttgctaa gaaccaagaa ttcaactatc 540
gagtatgatg gtattatgca gaaatctcaa gataatgtca cagaactgta cgacgtttcc 600
atcagcttgt ctgtttcatt ccctgatgtt acgagcaata tgaccatctt ctgtattctg 660
gaaactgaca agacgcggct tttatcttca cctttctcta tagagcttga ggaccctcag 720
cctcccccag gggtgacccc cagagtgccc ataacacaga acccctgtcc tccactcaaa 780
gagtgtcccc catgcgcagc tccagacctc ttgggtggac catccgtctt catcttccct 840
ccaaagatca aggatgtact catgatctcc ctgagcccca tggtcacatg tgtggtggtg 900
gatgtgagcg aggatgaccc agacgtccag atcagctggt ttgtgaacaa cgtggaagta 960
cacacagctc agacacaaac ccatagagag gattacaaca gtactctccg ggtggtcagt 1020
gccctcccca tccagcacca ggactggatg agtggcaagg agttcaaatg caaggtcaac 1080
aacagagccc tcccatcccc catcgagaaa accatctcaa aacccagagg gccagtaaga 1140
gctccacagg tatatgtctt gcctccacca gcagaagaga tgactaagaa agagttcagt 1200
ctgacctgca tgatcacagg cttcttacct gccgaaattg ctgtggactg gaccagcaat 1260
gggcgtacag agcaaaacta caagaacacc gcaacagtcc tggactctga tggttcttac 1320
ttcatgtaca gcaagctcag agtacaaaag agcacttggg aaagaggaag tcttttcgcc 1380
tgctcagtgg tccacgaggg tctgcacaat caccttacga ctaagaccat ctcccggtct 1440
ctgggtaaat ccggataa                                               1458

<210> 228
<211> 1455
<212> DNA
<213> Artificial Sequence

<220> 
<223> Human CD80-murine Ig fusion protein coding sequence

<400> 228
atgggccaca cacggaggca gggaacatca ccatccaagt gtccatacct caatttcttt 60
cagctcttgg tgctggctgg tctttctcac ttctgttcag gtgttatcca cgtgaccaag 120
gaagtgaaag aagtggcaac gctgtcctgt ggtcacaatg tttctgttga agagctggca 180
caaactcgca tctactggca aaaggagaag aaaatggtgc tgactatgat gtctggggac 240
atgaatatat ggcccgagta caagaaccgg accatctttg atatcactaa taacctctcc 300
atcgtgatcc tggctctgcg cccatctgac gagggcacat acgagtgtgt tgttctgaag 360
tatgaaaaag acgctttcaa gcgggaacac ctggctgaag tgacgttatc agtcaaagct 420
gacttcccta cacctagtat atctgacttt gaaattccaa cttctaatat tagaaggata 480
atttgctcaa cctctggagg ttttcctgag cctcacctct cctggctgga aaatggagaa 540
gaattaaatg ccatcaacac aacagtttcc caagatcctg aaactgagct ctatgctgtt 600
agcagcaaac tggatttcaa tatgacaacc aaccacagct tcatgtgtct catcaagtat 660
ggacatttaa gagtgaatca gaccttcaac tggaatacaa ccaagcaaga gcattttcct 720
gataacgggg tgacccccag agtgcccata acacagaacc cctgtcctcc actcaaagag 780
tgtcccccat gcgcagctcc agacctcttg ggtggaccat ccgtcttcat cttccctcca 840
aagatcaagg atgtactcat gatctccctg agccccatgg tcacatgtgt ggtggtggat 900
gtgagcgagg atgacccaga cgtccagatc agctggtttg tgaacaacgt ggaagtacac 960
acagctcaga cacaaaccca tagagaggat tacaacagta ctctccgggt ggtcagtgcc 1020
ctccccatcc agcaccagga ctggatgagt ggcaaggagt tcaaatgcaa ggtcaacaac 1080
agagccctcc catcccccat cgagaaaacc atctcaaaac ccagagggcc agtaagagct 1140
ccacaggtat atgtcttgcc tccaccagca gaagagatga ctaagaaaga gttcagtctg 1200
acctgcatga tcacaggctt cttacctgcc gaaattgctg tggactggac cagcaatggg 1260
cgtacagagc aaaactacaa gaacaccgca acagtcctgg actctgatgg ttcttacttc 1320
atgtacagca agctcagagt acaaaagagc acttgggaaa gaggaagtct tttcgcctgc 1380
tcagtggtcc acgagggtct gcacaatcac cttacgacta agaccatctc ccggtctctg 1440
ggtaaatccg gataa                                                  1455
