                         SEQUENCE LISTING

<110>  Cujec, Thomas P.
       Ho, Lillian
       Phuong, Trung
 
<120>  Anti-TNFRI Polypeptides and Their Uses

<130>  AMBX-0144.00PCT

<150>  60/981,467
<151>  2007-10-19

<160>  45    

<170>  PatentIn version 3.4

<210>  1
<211>  77
<212>  DNA
<213>  Methanococcus jannaschii

<400>  1
ccggcggtag ttcagcaggg cagaacggcg gactctaaat ccgcatggcg ctggttcaaa     60

tccggcccgc cggacca                                                    77


<210>  2
<211>  88
<212>  DNA
<213>  Artificial

<220>
<223>  An optimized amber supressor tRNA

<400>  2
cccagggtag ccaagctcgg ccaacggcga cggactctaa atccgttctc gtaggagttc     60

gagggttcga atcccttccc tgggacca                                        88


<210>  3
<211>  89
<212>  DNA
<213>  Artificial

<220>
<223>  An optimized AGGA frameshift supressor tRNA

<400>  3
gcgagggtag ccaagctcgg ccaacggcga cggacttcct aatccgttct cgtaggagtt     60

cgagggttcg aatccctccc ctcgcacca                                       89


<210>  4
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       p-azido-L-phenylalanine

<400>  4

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Tyr Tyr 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  5
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       p-benzoyl-L-phenylalanine

<400>  5

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Ser His 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  6
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  6

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Pro Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ala Ile Tyr 
145                 150                 155                 160 


Leu Ala Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  7
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  7

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Pro Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ile Pro Tyr 
145                 150                 155                 160 


Leu Pro Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  8
<211>  305
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       propargyl-phenylalanine

<400>  8

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Lys Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Ala Ile Tyr 
145                 150                 155                 160 


Leu Ala Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile His 
                165                 170                 175     


Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His Asn 
            180                 185                 190         


Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser Lys 
        195                 200                 205             


Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala Lys 
    210                 215                 220                 


Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro Ile 
225                 230                 235                 240 


Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys Arg 
                245                 250                 255     


Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu Leu 
            260                 265                 270         


Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys Asn 
        275                 280                 285             


Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys Arg 
    290                 295                 300                 


Leu 
305 


<210>  9
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       p-azido-phenylalanine

<400>  9

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Asn Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Leu His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  10
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  10

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Leu His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  11
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       p-azido-phenylalanine

<400>  11

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Val His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  12
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  12

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Thr 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Ser Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Pro Ser His 
145                 150                 155                 160 


Tyr Gln Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  13
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-acetyl-phenylalanine

<400>  13

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Cys His 
145                 150                 155                 160 


Tyr Arg Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  14
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of p-acetyl 
       -phenylalanine

<400>  14

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Leu 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Thr His 
145                 150                 155                 160 


Tyr Arg Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  15
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-acetyl-phenylalanine

<400>  15

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Glu Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Gly Gly His 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ile Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  16
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase  for the incorporation of 
       p-azido-phenylalanine

<400>  16

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Ala 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Arg Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Val Ile His 
145                 150                 155                 160 


Tyr Asp Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  17
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  Aminoacyl tRNA synthetase for the incorporation of 
       p-azido-phenylalanine

<400>  17

Met Asp Glu Phe Glu Met Ile Lys Arg Asn Thr Ser Glu Ile Ile Ser 
1               5                   10                  15      


Glu Glu Glu Leu Arg Glu Val Leu Lys Lys Asp Glu Lys Ser Ala Gly 
            20                  25                  30          


Ile Gly Phe Glu Pro Ser Gly Lys Ile His Leu Gly His Tyr Leu Gln 
        35                  40                  45              


Ile Lys Lys Met Ile Asp Leu Gln Asn Ala Gly Phe Asp Ile Ile Ile 
    50                  55                  60                  


Leu Leu Ala Asp Leu His Ala Tyr Leu Asn Gln Lys Gly Glu Leu Asp 
65                  70                  75                  80  


Glu Ile Arg Lys Ile Gly Asp Tyr Asn Lys Lys Val Phe Glu Ala Met 
                85                  90                  95      


Gly Leu Lys Ala Lys Tyr Val Tyr Gly Ser Thr Phe Gln Leu Asp Lys 
            100                 105                 110         


Asp Tyr Thr Leu Asn Val Tyr Arg Leu Ala Leu Lys Thr Thr Leu Lys 
        115                 120                 125             


Arg Ala Arg Arg Ser Met Glu Leu Ile Ala Arg Glu Asp Glu Asn Pro 
    130                 135                 140                 


Lys Val Ala Glu Val Ile Tyr Pro Ile Met Gln Val Asn Thr Tyr Tyr 
145                 150                 155                 160 


Tyr Leu Gly Val Asp Val Ala Val Gly Gly Met Glu Gln Arg Lys Ile 
                165                 170                 175     


His Met Leu Ala Arg Glu Leu Leu Pro Lys Lys Val Val Cys Ile His 
            180                 185                 190         


Asn Pro Val Leu Thr Gly Leu Asp Gly Glu Gly Lys Met Ser Ser Ser 
        195                 200                 205             


Lys Gly Asn Phe Ile Ala Val Asp Asp Ser Pro Glu Glu Ile Arg Ala 
    210                 215                 220                 


Lys Ile Lys Lys Ala Tyr Cys Pro Ala Gly Val Val Glu Gly Asn Pro 
225                 230                 235                 240 


Ile Met Glu Ile Ala Lys Tyr Phe Leu Glu Tyr Pro Leu Thr Ile Lys 
                245                 250                 255     


Arg Pro Glu Lys Phe Gly Gly Asp Leu Thr Val Asn Ser Tyr Glu Glu 
            260                 265                 270         


Leu Glu Ser Leu Phe Lys Asn Lys Glu Leu His Pro Met Asp Leu Lys 
        275                 280                 285             


Asn Ala Val Ala Glu Glu Leu Ile Lys Ile Leu Glu Pro Ile Arg Lys 
    290                 295                 300                 


Arg Leu 
305     


<210>  18
<211>  920
<212>  DNA
<213>  Artificial

<220>
<223>  Perimplasmic scFv-108 nucleotide sequence

<400>  18
accacaggtc tcccatgcga ttcagtacga cgctggcaac ggccgctacg gcactcttct     60

ttactgcaag tcaggtaagc gcgcaggttc agctgcagca gtctggagct gagctgatga    120

agcctggggc ctcagtgaag atatcctgca aggctactgg ctacacattc agtagttact    180

ggatagagtg ggtaaagcag aggcctggac atggccttga gtggattgga gagattttac    240

cgggaagtaa aaaaactaac tacaatgaga agttcaaggg aaaggccaca ttcactgcag    300

atacatcctc caacacagcc tacatgcaat ttagcagcct gacatctgag gactctgccg    360

tctattactg tgcaagatat tactatagga acgacgacta tggtatggac tactggggtc    420

aaggaacctc agtcaccgtc tcctcaggcg gcggcggctc cggtggtggt ggatctggtg    480

ggggcggcag cggcggtggc ggcagtgaaa tccacatgac acagactaca tcctccctgt    540

ctgcctctct gggagacaga gtcaccatca gttgcagtgc aagtcaggac atcaggaatt    600

atttaaactg gtatcagcag aaacctgatg gaactgttaa actcctgatc tattacacat    660

caactttaca ttcaggagtc ccatcaaggt tcagtggcag cgggtctggg acagattatt    720

ctctcaccat cagcaacctg gaacctgaag atattgccac ttattattgt cagcagtata    780

gtaagattcc gtacacgttc acagggggga ccaagctgga aataaaacgg gctgatgctg    840

cagaacaaaa actcatctca gaagaggatc tgaatagcgc cgtcgaccat catcatcacc    900

atcattaagg taccaaccaa                                                920


<210>  19
<211>  297
<212>  PRT
<213>  Artificial

<220>
<223>  Perimplasmic scFv-108 amino acid sequence

<400>  19

Met Arg Phe Ser Thr Thr Leu Ala Thr Ala Ala Thr Ala Leu Phe Phe 
1               5                   10                  15      


Thr Ala Ser Gln Val Ser Ala Gln Val Gln Leu Gln Gln Ser Gly Ala 
            20                  25                  30          


Glu Leu Met Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Thr 
        35                  40                  45              


Gly Tyr Thr Phe Ser Ser Tyr Trp Ile Glu Trp Val Lys Gln Arg Pro 
    50                  55                  60                  


Gly His Gly Leu Glu Trp Ile Gly Glu Ile Leu Pro Gly Ser Lys Lys 
65                  70                  75                  80  


Thr Asn Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Phe Thr Ala Asp 
                85                  90                  95      


Thr Ser Ser Asn Thr Ala Tyr Met Gln Phe Ser Ser Leu Thr Ser Glu 
            100                 105                 110         


Asp Ser Ala Val Tyr Tyr Cys Ala Arg Tyr Tyr Tyr Arg Asn Asp Asp 
        115                 120                 125             


Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
145                 150                 155                 160 


Gly Gly Gly Ser Glu Ile His Met Thr Gln Thr Thr Ser Ser Leu Ser 
                165                 170                 175     


Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln Asp 
            180                 185                 190         


Ile Arg Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val 
        195                 200                 205             


Lys Leu Leu Ile Tyr Tyr Thr Ser Thr Leu His Ser Gly Val Pro Ser 
    210                 215                 220                 


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser 
225                 230                 235                 240 


Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser 
                245                 250                 255     


Lys Ile Pro Tyr Thr Phe Thr Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            260                 265                 270         


Ala Asp Ala Ala Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Ser 
        275                 280                 285             


Ala Val Asp His His His His His His 
    290                 295         


<210>  20
<211>  779
<212>  DNA
<213>  Artificial

<220>
<223>  Cytoplasmic scFv nucleotide sequence

<400>  20
ccatgggcca ccaccaccac caccaccagg ttcagctgca gcagtctgga gctgagctga     60

tgaagcctgg ggcctcagtg aagatatcct gcaaggctac tggctacaca ttcagtagtt    120

actggataga gtgggtaaag cagaggcctg gacatggcct tgagtggatt ggagagattt    180

taccgggaag taaaaaaact aactacaatg agaagttcaa gggaaaggcc acattcactg    240

cagatacatc ctccaacaca gcctacatgc aatttagcag cctgacatct gaggactctg    300

ccgtctatta ctgtgcaaga tattactata ggaacgacga ctatggtatg gactactggg    360

gtcaaggaac ctcagtcacc gtctcctcag gcggcggcgg ctccggtggt ggtggatctg    420

gtgggggcgg cagcggcggt ggcggcagtg aaatccacat gacacagact acatcctccc    480

tgtctgcctc tctgggagac agagtcacca tcagttgcag tgcaagtcag gacatcagga    540

attatttaaa ctggtatcag cagaaacctg atggaactgt taaactcctg atctattaca    600

catcaacttt acattcagga gtcccatcaa ggttcagtgg cagcgggtct gggacagatt    660

attctctcac catcagcaac ctggaacctg aagatattgc cacttattat tgtcagcagt    720

atagtaagat tccgtacacg ttcacagggg ggaccaagct ggaaatataa aggtaccaa     779


<210>  21
<211>  255
<212>  PRT
<213>  Artificial

<220>
<223>  Cytoplasmic scFv amino acid sequence

<400>  21

Met Gly His His His His His His Gln Val Gln Leu Gln Gln Ser Gly 
1               5                   10                  15      


Ala Glu Leu Met Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala 
            20                  25                  30          


Thr Gly Tyr Thr Phe Ser Ser Tyr Trp Ile Glu Trp Val Lys Gln Arg 
        35                  40                  45              


Pro Gly His Gly Leu Glu Trp Ile Gly Glu Ile Leu Pro Gly Ser Lys 
    50                  55                  60                  


Lys Thr Asn Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Phe Thr Ala 
65                  70                  75                  80  


Asp Thr Ser Ser Asn Thr Ala Tyr Met Gln Phe Ser Ser Leu Thr Ser 
                85                  90                  95      


Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Tyr Tyr Tyr Arg Asn Asp 
            100                 105                 110         


Asp Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Glu Ile His Met Thr Gln Thr Thr Ser Ser Leu 
145                 150                 155                 160 


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln 
                165                 170                 175     


Asp Ile Arg Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
            180                 185                 190         


Val Lys Leu Leu Ile Tyr Tyr Thr Ser Thr Leu His Ser Gly Val Pro 
        195                 200                 205             


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
    210                 215                 220                 


Ser Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr 
225                 230                 235                 240 


Ser Lys Ile Pro Tyr Thr Phe Thr Gly Gly Thr Lys Leu Glu Ile 
                245                 250                 255 


<210>  22
<211>  1578
<212>  DNA
<213>  Artificial

<220>
<223>  Fab-108 nucleotide sequence

<400>  22
accaccggtc tcccatgaaa aaactgctgt tcgcgattcc gctggtggtg ccgttctata     60

gccatagcac catggagctc gaaatccaca tgacacagac tacatcctcc ctgtctgcct    120

ctctgggaga cagagtcacc atcagttgca gtgcaagtca ggacatcagg aattatttaa    180

actggtatca gcagaaacct gatggaactg ttaaactcct gatctattac acatcaactt    240

tacattcagg agtcccatca aggttcagtg gcagcgggtc tgggacagat tattctctca    300

ccatcagcaa cctggaacct gaagatattg ccacttatta ttgtcagcag tatagtaaga    360

ttccgtacac gttcacaggg gggaccaagc tggaaataaa acgggctgat gctgcaaagc    420

ttgagatcaa acgaactgtg gctgcaccat ctgtcttcat cttcccgcca tctgatgagc    480

agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat cccagagagg    540

ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag gagagtgtca    600

cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg ctgagcaaag    660

cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc ctgtcctcgc    720

ccgtcacaaa gagcttcaac aggggagagt gttaagctgg ggatcctcta gaggttgagg    780

tgattttatg aaaaagaata tcgcatttct tcttgcatct atgttcgttt tttctattgc    840

tacaaacgcg tacgctcagg ttcagctgct cgagcaggtt cagctgcagc agtctggagc    900

tgagctgatg aagcctgggg cctcagtgaa gatatcctgc aaggctactg gctacacatt    960

cagtagttac tggatagagt gggtaaagca gaggcctgga catggccttg agtggattgg   1020

agagatttta ccgggaagta aaaaaactaa ctacaatgag aagttcaagg gaaaggccac   1080

attcactgca gatacatcct ccaacacagc ctacatgcaa tttagcagcc tgacatctga   1140

ggactctgcc gtctattact gtgcaagata ttactatagg aacgacgact atggtatgga   1200

ctactggggt caaggaacct cagtcaccgt ctcctcagcg ggcccatcgg tcttccccct   1260

ggcaccctcc tccaagagca cctctggggg cacagcggcc ctgggctgcc tggtcaagga   1320

ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc gccctgacca gcggcgtgca   1380

caccttcccg gctgtcctac agtcctcagg actctactcc ctcagcagcg tggtgactgt   1440

gccctctagc agcttgggca cccagaccta catctgcaac gtgaatcaca agcccagcaa   1500

caccaaggtg gacaagaaag ttgagcccaa atcttgtgac aaacatcatc atcatcatca   1560

ttagggtacc accataac                                                 1578


<210>  23
<211>  246
<212>  PRT
<213>  Artificial

<220>
<223>  Translated protein sequence of VL Kappa chain of Fab 108

<400>  23

Met Lys Lys Leu Leu Phe Ala Ile Pro Leu Val Val Pro Phe Tyr Ser 
1               5                   10                  15      


His Ser Thr Met Glu Leu Glu Ile His Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser 
        35                  40                  45              


Gln Asp Ile Arg Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr Tyr Thr Ser Thr Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln 
            100                 105                 110         


Tyr Ser Lys Ile Pro Tyr Thr Phe Thr Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Lys Arg Ala Asp Ala Ala Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 
    130                 135                 140                 


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
145                 150                 155                 160 


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
                165                 170                 175     


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
            180                 185                 190         


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
        195                 200                 205             


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
    210                 215                 220                 


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
225                 230                 235                 240 


Phe Asn Arg Gly Glu Cys 
                245     


<210>  24
<211>  235
<212>  PRT
<213>  Artificial

<220>
<223>  translated protein sequence of VH-CH1 chain of Fab108

<400>  24

Gln Val Gln Leu Leu Glu Gln Val Gln Leu Gln Gln Ser Gly Ala Glu 
1               5                   10                  15      


Leu Met Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Thr Gly 
            20                  25                  30          


Tyr Thr Phe Ser Ser Tyr Trp Ile Glu Trp Val Lys Gln Arg Pro Gly 
        35                  40                  45              


His Gly Leu Glu Trp Ile Gly Glu Ile Leu Pro Gly Ser Lys Lys Thr 
    50                  55                  60                  


Asn Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Phe Thr Ala Asp Thr 
65                  70                  75                  80  


Ser Ser Asn Thr Ala Tyr Met Gln Phe Ser Ser Leu Thr Ser Glu Asp 
                85                  90                  95      


Ser Ala Val Tyr Tyr Cys Ala Arg Tyr Tyr Tyr Arg Asn Asp Asp Tyr 
            100                 105                 110         


Gly Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala 
        115                 120                 125             


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
    130                 135                 140                 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
145                 150                 155                 160 


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
                165                 170                 175     


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
            180                 185                 190         


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
        195                 200                 205             


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
    210                 215                 220                 


Lys Ser Cys Asp Lys His His His His His His 
225                 230                 235 


<210>  25
<211>  863
<212>  DNA
<213>  Artificial

<220>
<223>  Periplasmic scFv-4D5 (C-terminal His) nucleotide sequence

<400>  25
accacaggtc tcccatgcga ttcagtacga cgctggcaac ggccgctacg gcactcttct     60

ttactgcaag tcaggtaagc gcggaagttc aactggttga atctggtggc ggtctggtgc    120

aaccaggtgg ttctctgcgt ctgtcttgcg ccgcgtccgg tttcaacatt aaagacacct    180

acattcactg ggtgcgccag gctccgggta agggtctgga atgggtggcc cgcatttatc    240

caacgaatgg ctacacccgc tacgcggatt ccgtgaaagg ccgtttcacg attagcgctg    300

ataccagcaa gaacaccgct tatctgcaaa tgaacagcct gcgtgccgaa gacaccgccg    360

tgtactattg tagccgttgg ggcggtgatg gcttttacgc gatggactac tggggccagg    420

gtacgctggt cacggtttct tctggcggcg gcggctccgg tggtggtgga tctggtgggg    480

gcggcagcgg cggtggcggc agtgatattc aaatgactca atctccttcc tctctgtctg    540

caagcgttgg tgatcgtgtg accatcacct gtcgcgcatc ccaggatgta aataccgcag    600

tagcgtggta tcagcagaag ccgggtaaag caccaaaact gctgatttat tccgcgagct    660

ttctgtatag cggcgtacca agccgtttta gcggttctcg tagcggtact gattttaccc    720

tgaccatctc ctctctgcag cctgaggatt ttgcgaccta ttattgccag cagcattata    780

cgacgccgcc tacgtttggc cagggtacta aggtggaaat taaaagcggc catcatcatc    840

accatcatta aggtacccaa cca                                            863


<210>  26
<211>  278
<212>  PRT
<213>  Artificial

<220>
<223>  Periplasmic scFv-4D5 (C-terminal His) amino acid sequence

<400>  26

Met Arg Phe Ser Thr Thr Leu Ala Thr Ala Ala Thr Ala Leu Phe Phe 
1               5                   10                  15      


Thr Ala Ser Gln Val Ser Ala Glu Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Asn Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr 
65                  70                  75                  80  


Thr Arg Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp 
                85                  90                  95      


Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
            100                 105                 110         


Asp Thr Ala Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr 
        115                 120                 125             


Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
145                 150                 155                 160 


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
                165                 170                 175     


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val 
            180                 185                 190         


Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys 
        195                 200                 205             


Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg 
    210                 215                 220                 


Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
225                 230                 235                 240 


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr 
                245                 250                 255     


Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Gly 
            260                 265                 270         


His His His His His His 
        275             


<210>  27
<211>  869
<212>  DNA
<213>  Artificial

<220>
<223>  Periplasmic scFv-4D5 (N-terminal His) nucleotide sequence

<400>  27
accacaggtc tcccatgcga ttcagtacga cgctggcaac ggccgctacg gcactcttct     60

ttactgcaag tcaggtaagc gcgggccacc accaccacca ccactctggt gaagttcaac    120

tggttgaatc tggtggcggt ctggtgcaac caggtggttc tctgcgtctg tcttgcgccg    180

cgtccggttt caacattaaa gacacctaca ttcactgggt gcgccaggct ccgggtaagg    240

gtctggaatg ggtggcccgc atttatccaa cgaatggcta cacccgctac gcggattccg    300

tgaaaggccg tttcacgatt agcgctgata ccagcaagaa caccgcttat ctgcaaatga    360

acagcctgcg tgccgaagac accgccgtgt actattgtag ccgttggggc ggtgatggct    420

tttacgcgat ggactactgg ggccagggta cgctggtcac ggtttcttct ggcggcggcg    480

gctccggtgg tggtggatct ggtgggggcg gcagcggcgg tggcggcagt gatattcaaa    540

tgactcaatc tccttcctct ctgtctgcaa gcgttggtga tcgtgtgacc atcacctgtc    600

gcgcatccca ggatgtaaat accgcagtag cgtggtatca gcagaagccg ggtaaagcac    660

caaaactgct gatttattcc gcgagctttc tgtatagcgg cgtaccaagc cgttttagcg    720

gttctcgtag cggtactgat tttaccctga ccatctcctc tctgcagcct gaggattttg    780

cgacctatta ttgccagcag cattatacga cgccgcctac gtttggccag ggtactaagg    840

tggaaattaa aagctaaggt acccaacca                                      869


<210>  28
<211>  280
<212>  PRT
<213>  Artificial

<220>
<223>  Periplasmic scFv-4D5 (N-terminal His) amino acid sequence

<400>  28

Met Arg Phe Ser Thr Thr Leu Ala Thr Ala Ala Thr Ala Leu Phe Phe 
1               5                   10                  15      


Thr Ala Ser Gln Val Ser Ala Gly His His His His His His Ser Gly 
            20                  25                  30          


Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
        35                  40                  45              


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
    50                  55                  60                  


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
65                  70                  75                  80  


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
                85                  90                  95      


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
            100                 105                 110         


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
        115                 120                 125             


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
    130                 135                 140                 


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met 
                165                 170                 175     


Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
            180                 185                 190         


Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala Val Ala Trp Tyr 
        195                 200                 205             


Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser 
    210                 215                 220                 


Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly 
225                 230                 235                 240 


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala 
                245                 250                 255     


Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro Thr Phe Gly Gln 
            260                 265                 270         


Gly Thr Lys Val Glu Ile Lys Ser 
        275                 280 


<210>  29
<211>  1538
<212>  DNA
<213>  Artificial

<220>
<223>  Fab-4D5 nucleotide sequence

<400>  29
atgaaaaaac tgctgttcgc gattccgctg gtggtgccgt tctatagcca tagcaccatg     60

gagctcgata ttcaaatgac tcaatctcct tcctctctgt ctgcaagcgt tggtgatcgt    120

gtgaccatca cctgtcgcgc atcccaggat gtaaataccg cagtagcgtg gtatcagcag    180

aagccgggta aagcaccaaa actgctgatt tattccgcga gctttctgta tagcggcgta    240

ccaagccgtt ttagcggttc tcgtagcggt actgatttta ccctgaccat ctcctctctg    300

cagcctgagg attttgcgac ctattattgc cagcagcatt atacgacgcc gcctacgttt    360

ggccagggta ctaaggtgga aattaaaaag cttgagatca aacgaactgt ggctgcacca    420

tctgtcttca tcttcccgcc atctgatgag cagttgaaat ctggaactgc ctctgttgtg    480

tgcctgctga ataacttcta tcccagagag gccaaagtac agtggaaggt ggataacgcc    540

ctccaatcgg gtaactccca ggagagtgtc acagagcagg acagcaagga cagcacctac    600

agcctcagca gcaccctgac gctgagcaaa gcagactacg agaaacacaa agtctacgcc    660

tgcgaagtca cccatcaggg cctgtcctcg cccgtcacaa agagcttcaa caggggagag    720

tgttaagctg gggatcctct agaggttgag gtgattttat gaaaaagaat atcgcatttc    780

ttcttgcatc tatgttcgtt ttttctattg ctacaaacgc gtacgctcag gttcagctgc    840

tcgaggaagt tcaactggtt gaatctggtg gcggtctggt gcaaccaggt ggttctctgc    900

gtctgtcttg cgccgcgtcc ggtttcaaca ttaaagacac ctacattcac tgggtgcgcc    960

aggctccggg taagggtctg gaatgggtgg cccgcattta tccaacgaat ggctacaccc   1020

gctacgcgga ttccgtgaaa ggccgtttca cgattagcgc tgataccagc aagaacaccg   1080

cttatctgca aatgaacagc ctgcgtgccg aagacaccgc cgtgtactat tgtagccgtt   1140

ggggcggtga tggcttttac gcgatggact actggggcca gggtacgctg gtcacggttt   1200

cttctggggc ccaatcggtc ttccccctgg caccctcctc caagagcacc tctgggggca   1260

cagcggccct gggctgcctg gtcaaggact acttccccga accggtgacg gtgtcgtgga   1320

actcaggcgc cctgaccagc ggcgtgcaca ccttcccggc tgtcctacag tcctcaggac   1380

tctactccct cagcagcgtg gtgactgtgc cctctagcag cttgggcacc cagacctaca   1440

tctgcaacgt gaatcacaag cccagcaaca ccaaggtgga caagaaagtt gagcccaaat   1500

cttgtgacaa acatcatcat catcatcatt aaggtacc                           1538


<210>  30
<211>  219
<212>  PRT
<213>  Artificial

<220>
<223>  VL-kappa chain amino acid sequence

<400>  30

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  31
<211>  234
<212>  PRT
<213>  Artificial

<220>
<223>  VH-CH1 chain amino acid sequence

<400>  31

Gln Val Gln Leu Leu Glu Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
1               5                   10                  15      


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
            20                  25                  30          


Phe Asn Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly 
        35                  40                  45              


Lys Gly Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr 
    50                  55                  60                  


Arg Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr 
65                  70                  75                  80  


Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
                85                  90                  95      


Thr Ala Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala 
            100                 105                 110         


Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Ala 
        115                 120                 125             


Gln Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
    130                 135                 140                 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
145                 150                 155                 160 


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
                165                 170                 175     


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
            180                 185                 190         


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
        195                 200                 205             


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys 
    210                 215                 220                 


Ser Cys Asp Lys His His His His His His 
225                 230                 


<210>  32
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  forward primer LB7

<400>  32

Gly Ala Tyr Ala Thr Thr Cys Ala Gly Ala Thr Gly Ala Tyr Asp Cys 
1               5                   10                  15      


Ala Gly Thr Cys 
            20  


<210>  33
<211>  22
<212>  PRT
<213>  Artificial

<220>
<223>  primer mukappa2 hybridizing to the kappa constant region

<400>  33

Gly Gly Cys Ala Cys Cys Thr Cys Cys Ala Gly Ala Thr Gly Thr Thr 
1               5                   10                  15      


Ala Ala Cys Thr Gly Cys 
            20          


<210>  34
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  27.14 VH chain N-terminal sequence

<400>  34

Ile Gln Thr Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys 
1               5                   10                  15  


<210>  35
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer 27.14 VHF2

<400>  35
atccaaacrc agatccagtt gg                                              22


<210>  36
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  muIgG1-RT2 hybridizing to the mouse IgG2a

<400>  36
ttgtatctcc acacacaggg g                                               21


<210>  37
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer HB11

<400>  37
gaggtgcags kggtggagtc                                                 20


<210>  38
<211>  107
<212>  PRT
<213>  Artificial

<220>
<223>  Protein sequence of the mAb 27.14VL region

<400>  38

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln Asp Ile Asn Asn Tyr 
            20                  25                  30          


Leu Asn Trp Phe Gln Gln Arg Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Asn Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Thr Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Leu Gln Tyr Ser Asn Leu Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  39
<211>  122
<212>  PRT
<213>  Artificial

<220>
<223>  Protein sequence of the mAb 27.14VH region

<400>  39

Ile Gln Thr Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys 
1               5                   10                  15      


Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe 
            20                  25                  30          


Thr Asp Tyr Ser Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
        35                  40                  45              


Lys Trp Met Gly Trp Ile Lys Thr Ala Thr Gly Glu Pro Thr Tyr Ala 
    50                  55                  60                  


Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser 
65                  70                  75                  80  


Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Phe Cys Ser Ser Leu Met Val Thr Asp Trp Tyr Phe Asp Val Trp 
            100                 105                 110         


Gly Ala Gly Thr Thr Val Thr Val Ser Ser 
        115                 120         


<210>  40
<211>  321
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of the mAb 27.14VL region

<400>  40
gacattcaga tgactcagac tacatcctcc ctgtctgcct ctctgggaga cagagtcacc     60

atcagttgca gtgcaagtca ggacattaac aattatttaa actggtttca gcagagacca    120

gatggaactg ttaaactcct gatctactac acatcaaatt tacactcagg agtcccttca    180

aggttcagtg gcagtgggtc tgggacagat tattctctca ccatcagcac cctggaacct    240

gaagatattg ccacttacta ttgtctgcag tatagtaacc ttccgtggac gttcggtgga    300

ggcaccaagc tggaaatcaa a                                              321


<210>  41
<211>  366
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of the mAb 27.14VH region

<400>  41
atccaaacgc agatccagtt ggtgcagtct ggacctgagc tgaagaagcc tggagagaca     60

gtcaagatct cctgcaaggc ttctggttat tccttcacag actattcaat gcactgggtg    120

aggcaggctc caggaaaggg tttaaagtgg atgggctgga taaaaactgc gactggtgag    180

ccaacatatg cagatgactt caagggacgg tttgccttct ctttggaaac ctctgccagc    240

actgcctatt tgcagatcaa caacctcaaa aatgaggaca cggcaacata tttctgttct    300

agtcttatgg ttacggactg gtatttcgat gtctggggcg cagggaccac ggtcaccgtc    360

tcctca                                                               366


<210>  42
<211>  106
<212>  PRT
<213>  Artificial

<220>
<223>  sequence of the mAb 155.241 VL region

<400>  42

Asp Ile Leu Leu Thr Gln Ser Pro Ala Ile Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Val Ser Phe Ser Cys Arg Ala Ser Gln Ser Ile Gly Thr Ser 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Arg Arg Asn Gly Ser Pro Arg Leu Leu Ile 
        35                  40                  45              


Lys Tyr Val Ser Glu Ser Ile Ser Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn Ser Val Glu Ser 
65                  70                  75                  80  


Glu Asp Ile Ala Asp Tyr Tyr Cys Gln Gln Ser Ser Gly Trp Pro Thr 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105     


<210>  43
<211>  117
<212>  PRT
<213>  Artificial

<220>
<223>  sequence of the mAb 155.241 VH region

<400>  43

Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Arg Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Ser Phe 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Thr Pro Glu Arg Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Ser Asp Gly Ser Ser Phe Ile Tyr Tyr Ala Asp Thr Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Pro Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Thr Ser Leu Arg Ser Glu Asp Ser Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Asp Gly Tyr Pro Phe Asp Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Leu Thr Val Ser Ser 
        115         


<210>  44
<211>  321
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of the mAb 155.241 VL region

<400>  44
gatattttgc tgactcagtc tccagccatc ctgtctgtga gtccaggaga aagagtcagt     60

ttctcctgca gggccagtca gagcattggc acaagcatac actggtatca gcaaagaaga    120

aatggttctc caaggcttct cataaagtat gtttctgagt ctatctctgg gatcccttcc    180

aggtttagtg gcagtggatc agggacagat tttactctta gcatcaacag tgtggagtct    240

gaagatattg cagattatta ctgtcaacaa agtagtggct ggccaactac gttcggtgct    300

gggaccaagc tggagctgaa a                                              321


<210>  45
<211>  351
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of the mAb 155.241 VH region

<400>  45
gatgtgcaac tggtggagtc tgggggaggc ttagtgcagc ctggagggtc ccggaaactc     60

tcctgtgcag cctctggatt cactttcact agctttggaa tgcactgggt tcgtcagact    120

ccagagaggg ggctggagtg ggtcgcatac attagtgatg gcagtagttt catctactat    180

gcagacacag tgaagggccg attcaccatc tccagagaca atcccaagaa caccctgttc    240

ctgcaaatga ccagtctaag gtctgaggac tcggccatgt attactgtgc aagaggggat    300

ggttacccct ttgactactg gggccaaggc accactctca cagtctcctc a             351


