                         SEQUENCE LISTING

<110>  Chang, Yung-Fu
 
<120>  COMPOSITIONS FOR ELICITING AN IMMUNE RESPONSE AGAINST 
       MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS

<130>  1258_4222PCT

<150>  US 61/094,552
<151>  2008-09-05

<150>  US 60/979,822
<151>  2007-10-13

<160>  33    

<170>  PatentIn version 3.4

<210>  1
<211>  788
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Map74F protein

<400>  1

Leu Asn Gln Ser Val Ser Ala Thr Asp Thr Leu Thr Gly Ala Gln Glu 
1               5                   10                  15      


Asn Leu Gly Gly Leu Ile Gln Ala Asp Ala Pro Ile Lys Pro Gly Asp 
            20                  25                  30          


Ser Gly Gly Pro Met Val Asn Ser Ala Gly Gln Val Ile Gly Val Asp 
        35                  40                  45              


Thr Ala Ala Thr Asp Ser Tyr Lys Met Ser Gly Gly Gln Gly Phe Ala 
    50                  55                  60                  


Ile Pro Ile Gly Arg Ala Met Ala Val Ala Asn Gln Ile Arg Ser Gly 
65                  70                  75                  80  


Ala Gly Ser Asn Thr Val His Ile Gly Pro Thr Ala Phe Leu Gly Leu 
                85                  90                  95      


Gly Val Thr Asp Asn Asn Gly Asn Gly Ala Arg Val Gln Arg Val Val 
            100                 105                 110         


Asn Thr Gly Pro Ala Ala Ala Ala Gly Ile Ala Pro Gly Asp Val Ile 
        115                 120                 125             


Thr Gly Val Asp Thr Val Pro Ile Asn Gly Ala Thr Ser Met Thr Glu 
    130                 135                 140                 


Val Leu Val Pro His His Pro Gly Asp Thr Ile Ala Val His Phe Arg 
145                 150                 155                 160 


Ser Val Asp Gly Gly Glu Arg Thr Ala Asn Ile Thr Leu Ala Glu Gly 
                165                 170                 175     


Pro Pro Ala Met Phe Tyr Gly Ala Phe Pro Pro Glu Phe Asn Ser Gly 
            180                 185                 190         


Arg Met Tyr Ser Gly Pro Gly Ala Gly Ser Phe Val Ala Ala Ala Thr 
        195                 200                 205             


Ala Trp Gln Asn Leu Ala Ala Glu Leu Gln Ser Ala Ala Ala Ser Tyr 
    210                 215                 220                 


Ser Thr Val Leu Ser Gly Leu Thr Ala Gly Pro Trp Val Gly Pro Ser 
225                 230                 235                 240 


Ser Leu Ala Met Ala Ser Ala Ala Ala Pro Tyr Val Ala Trp Met Gln 
                245                 250                 255     


Gln Thr Ala Ala Gln Ala Ala Glu Thr Ala Ala Gln Ala Thr Ala Ala 
            260                 265                 270         


Ala Thr Ala Tyr Glu Thr Ala Phe Ala Ala His Val Pro Pro Ala Val 
        275                 280                 285             


Ile Thr Glu Asn Arg Ala Leu Leu Ala Gln Leu Val Ala Thr Asn Ile 
    290                 295                 300                 


Phe Gly Gln Asn Thr Ala Ala Ile Ala Ala Asn Glu Ala Gln Tyr Gly 
305                 310                 315                 320 


Glu Phe Trp Ala Gln Asp Ala Thr Ala Met Asp Thr Tyr Phe Ala Ala 
                325                 330                 335     


Ser Ala Thr Ala Ala Asn Lys Leu Thr Glu Phe Gly Pro Ala Pro Lys 
            340                 345                 350         


Thr Thr Asn Glu Ala Ala Gln Pro Met Gln Ala Ala Ala Val Ser Ser 
        355                 360                 365             


Ala Ala Ser Thr Pro Ala Ala Asn Val Ala Gln Thr Ala Ala Ser Ala 
    370                 375                 380                 


Ala Ser Thr Thr Leu Pro Tyr Ser Gly Pro Phe Ser Gly Pro Ala Asn 
385                 390                 395                 400 


Leu Ala Tyr Leu Tyr Gln Thr Phe Met Thr Asn Leu Phe Asn Thr Val 
                405                 410                 415     


Pro Gly Gly Ala Ser Phe Tyr Thr Ser Met Tyr Asn Ala Val Lys Val 
            420                 425                 430         


Pro Leu Gly Leu Thr Thr Gln Phe Asn Asp Val Gly Leu Leu Val Asn 
        435                 440                 445             


Phe Pro Leu Ser Gln Trp Leu Lys Phe Ala Pro Pro Ile Gly Tyr Gly 
    450                 455                 460                 


Ala Leu Pro Lys Asp Ala Leu Gly Ala Gly Leu Gly Ala Leu Gly Phe 
465                 470                 475                 480 


Gly Arg Gly Thr Leu Tyr Ser Ala Ile Ser Pro Val Ala Gly Met Gly 
                485                 490                 495     


Asn Ala Gly Thr Leu Val Gly Lys Leu Ser Ile Pro Pro Ser Trp Ala 
            500                 505                 510         


Thr Ala Thr Pro Ser Ile Arg Thr Val Ala Ala Ala Leu Ser Ala Ala 
        515                 520                 525             


Gly Ala Glu Ala Val Pro Ala Ala Ala Leu Gly Glu Gly Ser Leu Phe 
    530                 535                 540                 


Ser Ser Met Gly Leu Ala Gly Met Leu Gly Ser Ala Val Gly Ser Gly 
545                 550                 555                 560 


Gly Pro Thr Met Val Arg Gly Gly Val Arg Asn Arg Met Thr Ala Ile 
                565                 570                 575     


Lys Asp Leu Lys Asp Lys Gln Ser Pro Glu Gln Leu Lys Arg Leu Val 
            580                 585                 590         


Ala Gln Ile Ser Glu Gln Pro Glu Ser Val Gln His His Asn Val Asp 
        595                 600                 605             


Gln Glu Asn Leu Asp Ala Leu Leu Glu Gln Leu Ala Lys Lys Pro Gly 
    610                 615                 620                 


Ile His Ala Val His Leu Lys Lys Gly Asp Lys Ser Lys Val Leu Gly 
625                 630                 635                 640 


Leu Ala Pro Ala Ser Ala Ala Pro Ser Gly Leu Ala Leu Asp Arg Phe 
                645                 650                 655     


Ala Asp Arg Pro Leu Ala Pro Ile Asp Pro Ser Ala Met Val Gly Gln 
            660                 665                 670         


Val Gly Pro Gln Val Val Asn Ile Asp Thr Lys Phe Gly Tyr Asn Asn 
        675                 680                 685             


Ala Val Gly Ala Gly Thr Gly Ile Val Ile Asp Pro Asn Gly Val Val 
    690                 695                 700                 


Leu Thr Asn Asn His Val Ile Ser Gly Ala Thr Glu Ile Ser Ala Phe 
705                 710                 715                 720 


Asp Val Gly Asn Gly Gln Thr Tyr Ala Val Asp Val Val Gly Tyr Asp 
                725                 730                 735     


Arg Thr Gln Asp Ile Ala Val Leu Gln Leu Arg Gly Ala Ala Gly Leu 
            740                 745                 750         


Pro Thr Ala Thr Ile Gly Gly Glu Ala Thr Val Gly Glu Pro Ile Val 
        755                 760                 765             


Ala Leu Gly Asn Val Gly Gly Gln Gly Gly Thr Pro Asn Ala Val Ala 
    770                 775                 780                 


Gly Lys Val Ala 
785             


<210>  2
<211>  361
<212>  PRT
<213>  MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS

<400>  2

Met Ser Lys Ser His His His Arg Ser Val Trp Trp Ser Trp Leu Val 
1               5                   10                  15      


Gly Val Leu Thr Val Val Gly Leu Gly Leu Gly Leu Gly Ser Gly Val 
            20                  25                  30          


Gly Leu Ala Pro Ala Ser Ala Ala Pro Ser Gly Leu Ala Leu Asp Arg 
        35                  40                  45              


Phe Ala Asp Arg Pro Leu Ala Pro Ile Asp Pro Ser Ala Met Val Gly 
    50                  55                  60                  


Gln Val Gly Pro Gln Val Val Asn Ile Asp Thr Lys Phe Gly Tyr Asn 
65                  70                  75                  80  


Asn Ala Val Gly Ala Gly Thr Gly Ile Val Ile Asp Pro Asn Gly Val 
                85                  90                  95      


Val Leu Thr Asn Asn His Val Ile Ser Gly Ala Thr Glu Ile Ser Ala 
            100                 105                 110         


Phe Asp Val Gly Asn Gly Gln Thr Tyr Ala Val Asp Val Val Gly Tyr 
        115                 120                 125             


Asp Arg Thr Gln Asp Ile Ala Val Leu Gln Leu Arg Gly Ala Ala Gly 
    130                 135                 140                 


Leu Pro Thr Ala Thr Ile Gly Gly Glu Ala Thr Val Gly Glu Pro Ile 
145                 150                 155                 160 


Val Ala Leu Gly Asn Val Gly Gly Gln Gly Gly Thr Pro Asn Ala Val 
                165                 170                 175     


Ala Gly Lys Val Val Ala Leu Asn Gln Ser Val Ser Ala Thr Asp Thr 
            180                 185                 190         


Leu Thr Gly Ala Gln Glu Asn Leu Gly Gly Leu Ile Gln Ala Asp Ala 
        195                 200                 205             


Pro Ile Lys Pro Gly Asp Ser Gly Gly Pro Met Val Asn Ser Ala Gly 
    210                 215                 220                 


Gln Val Ile Gly Val Asp Thr Ala Ala Thr Asp Ser Tyr Lys Met Ser 
225                 230                 235                 240 


Gly Gly Gln Gly Phe Ala Ile Pro Ile Gly Arg Ala Met Ala Val Ala 
                245                 250                 255     


Asn Gln Ile Arg Ser Gly Ala Gly Ser Asn Thr Val His Ile Gly Pro 
            260                 265                 270         


Thr Ala Phe Leu Gly Leu Gly Val Thr Asp Asn Asn Gly Asn Gly Ala 
        275                 280                 285             


Arg Val Gln Arg Val Val Asn Thr Gly Pro Ala Ala Ala Ala Gly Ile 
    290                 295                 300                 


Ala Pro Gly Asp Val Ile Thr Gly Val Asp Thr Val Pro Ile Asn Gly 
305                 310                 315                 320 


Ala Thr Ser Met Thr Glu Val Leu Val Pro His His Pro Gly Asp Thr 
                325                 330                 335     


Ile Ala Val His Phe Arg Ser Val Asp Gly Gly Glu Arg Thr Ala Asn 
            340                 345                 350         


Ile Thr Leu Ala Glu Gly Pro Pro Ala 
        355                 360     


<210>  3
<211>  467
<212>  PRT
<213>  MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS

<400>  3

Met Phe Tyr Gly Ala Phe Pro Pro Glu Phe Asn Ser Gly Arg Met Tyr 
1               5                   10                  15      


Ser Gly Pro Gly Ala Gly Ser Phe Val Ala Ala Ala Thr Ala Trp Gln 
            20                  25                  30          


Asn Leu Ala Ala Glu Leu Gln Ser Ala Ala Ala Ser Tyr Ser Thr Val 
        35                  40                  45              


Leu Ser Gly Leu Thr Ala Gly Pro Trp Val Gly Pro Ser Ser Leu Ala 
    50                  55                  60                  


Met Ala Ser Ala Ala Ala Pro Tyr Val Ala Trp Met Gln Gln Thr Ala 
65                  70                  75                  80  


Ala Gln Ala Ala Glu Thr Ala Ala Gln Ala Thr Ala Ala Ala Thr Ala 
                85                  90                  95      


Tyr Glu Thr Ala Phe Ala Ala His Val Pro Pro Ala Val Ile Thr Glu 
            100                 105                 110         


Asn Arg Ala Leu Leu Ala Gln Leu Val Ala Thr Asn Ile Phe Gly Gln 
        115                 120                 125             


Asn Thr Ala Ala Ile Ala Ala Asn Glu Ala Gln Tyr Gly Glu Phe Trp 
    130                 135                 140                 


Ala Gln Asp Ala Thr Ala Met Asp Thr Tyr Phe Ala Ala Ser Ala Thr 
145                 150                 155                 160 


Ala Ala Asn Lys Leu Thr Glu Phe Gly Pro Ala Pro Lys Thr Thr Asn 
                165                 170                 175     


Glu Ala Ala Gln Pro Met Gln Ala Ala Ala Val Ser Ser Ala Ala Ser 
            180                 185                 190         


Thr Pro Ala Ala Asn Val Ala Gln Thr Ala Ala Ser Ala Ala Ser Thr 
        195                 200                 205             


Thr Leu Pro Tyr Ser Gly Pro Phe Ser Gly Pro Ala Asn Leu Ala Tyr 
    210                 215                 220                 


Leu Tyr Gln Thr Phe Met Thr Asn Leu Phe Asn Thr Val Pro Gly Gly 
225                 230                 235                 240 


Ala Ser Phe Tyr Thr Ser Met Tyr Asn Ala Val Lys Val Pro Leu Gly 
                245                 250                 255     


Leu Thr Thr Gln Phe Asn Asp Val Gly Leu Leu Val Asn Phe Pro Leu 
            260                 265                 270         


Ser Gln Trp Leu Lys Phe Ala Pro Pro Ile Gly Tyr Gly Ala Leu Pro 
        275                 280                 285             


Lys Asp Ala Leu Gly Ala Gly Leu Gly Ala Leu Gly Phe Gly Arg Gly 
    290                 295                 300                 


Thr Leu Tyr Ser Ala Ile Ser Pro Val Ala Gly Met Gly Asn Ala Gly 
305                 310                 315                 320 


Thr Leu Val Gly Lys Leu Ser Ile Pro Pro Ser Trp Ala Thr Ala Thr 
                325                 330                 335     


Pro Ser Ile Arg Thr Val Ala Ala Ala Leu Ser Ala Ala Gly Ala Glu 
            340                 345                 350         


Ala Val Pro Ala Ala Ala Leu Gly Glu Gly Ser Leu Phe Ser Ser Met 
        355                 360                 365             


Gly Leu Ala Gly Met Leu Gly Ser Ala Val Gly Ser Gly Gly Pro Thr 
    370                 375                 380                 


Met Val Arg Gly Gly Val Arg Asn Arg Met Thr Ala Ile Lys Asp Leu 
385                 390                 395                 400 


Lys Asp Lys Gln Ser Pro Glu Gln Leu Lys Arg Leu Val Ala Gln Ile 
                405                 410                 415     


Ser Glu Gln Pro Glu Ser Val Gln His His Asn Val Asp Gln Glu Asn 
            420                 425                 430         


Leu Asp Ala Leu Leu Glu Gln Leu Ala Lys Lys Pro Gly Ile His Ala 
        435                 440                 445             


Val His Leu Lys Lys Gly Asp Lys Ser Lys Val Leu Pro Ala Asp Ala 
    450                 455                 460                 


Gln Leu Gly 
465         


<210>  4
<211>  44
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR amplification primer

<400>  4
tacatatgca tcatcatcat catcatctca accagagcgt ctcg                      44


<210>  5
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  5
tagaattcgg ccggcggccc ctccgcc                                         27


<210>  6
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  6
ctaatcgaat tcatgttcta tggggccttt c                                    31


<210>  7
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  7
taatcgatat ccaggacctt ggacttgtc                                       29


<210>  8
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  8
atgatatcgg gctggcgccg gcgtcc                                          26


<210>  9
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer

<400>  9
atctcgagtc acgcgacctt gccggc                                          26


<210>  10
<211>  23
<212>  DNA
<213>  Mouse

<400>  10
cctgagcagg atggagaatt aca                                             23


<210>  11
<211>  19
<212>  DNA
<213>  Mouse

<400>  11
tccagaacat gccgcagag                                                  19


<210>  12
<211>  25
<212>  DNA
<213>  mouse

<400>  12
cccaagcagg ccacagaatt gaaag                                           25


<210>  13
<211>  20
<212>  DNA
<213>  mouse

<400>  13
ggaagcacgg cagcagaata                                                 20


<210>  14
<211>  24
<212>  DNA
<213>  mouse

<400>  14
aacttgaggg agaagtagga atgg                                            24


<210>  15
<211>  24
<212>  DNA
<213>  mouse

<400>  15
catcatcaaa ccagacccgc ccaa                                            24


<210>  16
<211>  25
<212>  DNA
<213>  mouse

<400>  16
catcttctca aaattcgagt gacaa                                           25


<210>  17
<211>  23
<212>  DNA
<213>  mouse

<400>  17
tgggagtaga caaggtacaa ccc                                             23


<210>  18
<211>  26
<212>  DNA
<213>  mouse

<400>  18
cacgtcgtag caaaccacca agtgga                                          26


<210>  19
<211>  24
<212>  DNA
<213>  mouse

<400>  19
tcaagtggca tagatgtgga agaa                                            24


<210>  20
<211>  21
<212>  DNA
<213>  mouse

<400>  20
tggctctgca ggattttcat g                                               21


<210>  21
<211>  27
<212>  DNA
<213>  mouse

<400>  21
tcaccatcct tttgccagtt cctccag                                         27


<210>  22
<211>  19
<212>  DNA
<213>  mouse

<400>  22
tcaccaccat ggagaaggc                                                  19


<210>  23
<211>  20
<212>  DNA
<213>  mouse

<400>  23
gctaagcagt tggtggtgca                                                 20


<210>  24
<211>  25
<212>  DNA
<213>  mouse

<400>  24
atgcccccat gtttgtgatg ggtgt                                           25


<210>  25
<211>  21
<212>  DNA
<213>  Bovine

<400>  25
caaattccgg tggatgatct g                                               21


<210>  26
<211>  22
<212>  DNA
<213>  Bovine

<400>  26
gcgacaggtc attcatcacc tt                                              22


<210>  27
<211>  28
<212>  DNA
<213>  bovine

<400>  27
atccagcgca aagccataaa tgaactca                                        28


<210>  28
<211>  23
<212>  DNA
<213>  bovine

<400>  28
ccaggatggt gactcgacta gac                                             23


<210>  29
<211>  20
<212>  DNA
<213>  bovine

<400>  29
tggctctgct ctcccagaac                                                 20


<210>  30
<211>  28
<212>  DNA
<213>  bovine

<400>  30
ccgacataaa cctctgaaat ccgaccca                                        28


<210>  31
<211>  18
<212>  DNA
<213>  bovine

<400>  31
gcccctctga accccaaa                                                   18


<210>  32
<211>  23
<212>  DNA
<213>  bovine

<400>  32
gcaggagtgt tgaaagtctc gaa                                             23


<210>  33
<211>  27
<212>  DNA
<213>  bovine

<400>  33
ccaaccgtga gaagatgacc cagatca                                         27


