                         SEQUENCE LISTING

<110>  University of Alabama
 
<120>  HIGH-THROUGHPUT REVERSION MUTATION METHODS FOR IDENTIFYING 
       CYTIDINE DEAMINASE INHIBITORS

<130>  222104-2110

<140>  60/977,101
<141>  2008-10-03

<150>  60/977,101
<151>  2007-10-03

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  558
<212>  DNA
<213>  Gaussia princeps

<400>  1
atgggagtca aagttctgtt tgccctgatc tgcatcgctg tggccgaggc caagcccacc       60

gagaacaacg aagacttcaa catcgtggcc gtggccagca acttcgcgac cacggatctc      120

gatgctgacc gcgggaagtt gcccggcaag aagctgccgc tggaggtgct caaagagatg      180

gaagccaatg cccggaaagc tggctgcacc aggggctgtc tgatctgcct gtcccacatc      240

aagtgcacgc ccaagatgaa gaagttcatc ccaggacgct gccacaccta tgaaggcgac      300

aaagagtccg cacagggcgg cataggcgag gcgatcgtcg acattcctga gattcctggg      360

ttcaaggact tggagcccat ggagcagttc atcgcacagg tcgatctgtg tgtggactgc      420

acaactggct gcctcaaagg gcttgccaac gtgcagtgtt ctgacctgct caagaagtgg      480

ctgccgcaac gctgtgcgac ctttgccagc aagatccagg gccaggtgga caagatcaag      540

ggggccggtg gtgactaa                                                    558


<210>  2
<211>  185
<212>  PRT
<213>  Gaussia princeps

<400>  2

Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 
1               5                   10                  15      


Ala Lys Pro Thr Glu Asn Asn Glu Asp Phe Asn Ile Val Ala Val Ala 
            20                  25                  30          


Ser Asn Phe Ala Thr Thr Asp Leu Asp Ala Asp Arg Gly Lys Leu Pro 
        35                  40                  45              


Gly Lys Lys Leu Pro Leu Glu Val Leu Lys Glu Met Glu Ala Asn Ala 
    50                  55                  60                  


Arg Lys Ala Gly Cys Thr Arg Gly Cys Leu Ile Cys Leu Ser His Ile 
65                  70                  75                  80  


Lys Cys Thr Pro Lys Met Lys Lys Phe Ile Pro Gly Arg Cys His Thr 
                85                  90                  95      


Tyr Glu Gly Asp Lys Glu Ser Ala Gln Gly Gly Ile Gly Glu Ala Ile 
            100                 105                 110         


Val Asp Ile Pro Glu Ile Pro Gly Phe Lys Asp Leu Glu Pro Met Glu 
        115                 120                 125             


Gln Phe Ile Ala Gln Val Asp Leu Cys Val Asp Cys Thr Thr Gly Cys 
    130                 135                 140                 


Leu Lys Gly Leu Ala Asn Val Gln Cys Ser Asp Leu Leu Lys Lys Trp 
145                 150                 155                 160 


Leu Pro Gln Arg Cys Ala Thr Phe Ala Ser Lys Ile Gln Gly Gln Val 
                165                 170                 175     


Asp Lys Ile Lys Gly Ala Gly Gly Asp 
            180                 185 


<210>  3
<211>  558
<212>  DNA
<213>  Gaussia princeps

<400>  3
acgggagtca aagttctgtt tgccctgatc tgcatcgctg tggccgaggc caagcccacc       60

gagaacaacg aagacttcaa catcgtggcc gtggccagca acttcgcgac cacggatctc      120

gatgctgacc gcgggaagtt gcccggcaag aagctgccgc tggaggtgct caaagagatg      180

gaagccaatg cccggaaagc tggctgcacc aggggctgtc tgatctgcct gtcccacatc      240

aagtgcacgc ccaagatgaa gaagttcatc ccaggacgct gccacaccta tgaaggcgac      300

aaagagtccg cacagggcgg cataggcgag gcgatcgtcg acattcctga gattcctggg      360

ttcaaggact tggagcccat ggagcagttc atcgcacagg tcgatctgtg tgtggactgc      420

acaactggct gcctcaaagg gcttgccaac gtgcagtgtt ctgacctgct caagaagtgg      480

ctgccgcaac gctgtgcgac ctttgccagc aagatccagg gccaggtgga caagatcaag      540

ggggccggtg gtgactaa                                                    558


<210>  4
<211>  558
<212>  DNA
<213>  Gaussia princeps

<400>  4
atgggagtca aagttctgtt tgccctgatc tgcatcgctg tggccgaggc caagcccacc       60

gagaacaacg aagacttcaa catcgtggcc gtggccagca acttcgcgac cacggatctc      120

gatgctgacc gcgggaagtt gcccggcaag aagctgccgc tggaggtgct caaagagatg      180

gaagccaatg cccggaaagc tggctgcacc aggggctgtc tgatctgcct gtcccacatc      240

aagtgcacgc ccaagatgaa gaagttcatc ccaggacgct gccacaccta ggaaggcgac      300

aaagagtccg cacagggcgg cataggcgag gcgatcgtcg acattcctga gattcctggg      360

ttcaaggact tggagcccat ggagcagttc atcgcacagg tcgatctgtg tgtggactgc      420

acaactggct gcctcaaagg gcttgccaac gtgcagtgtt ctgacctgct caagaagtgg      480

ctgccgcaac gctgtgcgac ctttgccagc aagatccagg gccaggtgga caagatcaag      540

ggggccggtg gtgactaa                                                    558


<210>  5
<211>  597
<212>  DNA
<213>  Homo sapiens

<400>  5
atggacagcc tcttgatgaa ccggaggaag tttctttacc aattcaaaaa tgtccgctgg       60

gctaagggtc ggcgtgagac ctacctgtgc tacgtagtga agaggcgtga cagtgctaca      120

tccttttcac tggactttgg ttatcttcgc aataagaacg gctgccacgt ggaattgctc      180

ttcctccgct acatctcgga ctgggaccta gaccctggcc gctgctaccg cgtcacctgg      240

ttcacctcct ggagcccctg ctacgactgt gcccgacatg tggccgactt tctgcgaggg      300

aaccccaacc tcagtctgag gatcttcacc gcgcgcctct acttctgtga ggaccgcaag      360

gctgagcccg aggggctgcg gcggctgcac cgcgccgggg tgcaaatagc catcatgacc      420

ttcaaagatt atttttactg ctggaatact tttgtagaaa accatgaaag aactttcaaa      480

gcctgggaag ggctgcatga aaattcagtt cgtctctcca gacagcttcg gcgcatcctt      540

ttgcccctgt atgaggttga tgacttacga gacgcatttc gtactttggg actttga         597


<210>  6
<211>  198
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Asp Ser Leu Leu Met Asn Arg Arg Lys Phe Leu Tyr Gln Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg Arg Glu Thr Tyr Leu Cys Tyr Val 
            20                  25                  30          


Val Lys Arg Arg Asp Ser Ala Thr Ser Phe Ser Leu Asp Phe Gly Tyr 
        35                  40                  45              


Leu Arg Asn Lys Asn Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr 
    50                  55                  60                  


Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp 
65                  70                  75                  80  


Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala Arg His Val Ala Asp 
                85                  90                  95      


Phe Leu Arg Gly Asn Pro Asn Leu Ser Leu Arg Ile Phe Thr Ala Arg 
            100                 105                 110         


Leu Tyr Phe Cys Glu Asp Arg Lys Ala Glu Pro Glu Gly Leu Arg Arg 
        115                 120                 125             


Leu His Arg Ala Gly Val Gln Ile Ala Ile Met Thr Phe Lys Asp Tyr 
    130                 135                 140                 


Phe Tyr Cys Trp Asn Thr Phe Val Glu Asn His Glu Arg Thr Phe Lys 
145                 150                 155                 160 


Ala Trp Glu Gly Leu His Glu Asn Ser Val Arg Leu Ser Arg Gln Leu 
                165                 170                 175     


Arg Arg Ile Leu Leu Pro Leu Tyr Glu Val Asp Asp Leu Arg Asp Ala 
            180                 185                 190         


Phe Arg Thr Leu Gly Leu 
        195             


<210>  7
<211>  5764
<212>  DNA
<213>  Artificial

<220>
<223>  pCMV-GLuc construct

<400>  7
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc      900

gagctcggat ccagccacca tgggagtcaa agttctgttt gccctgatct gcatcgctgt      960

ggccgaggcc aagcccaccg agaacaacga agacttcaac atcgtggccg tggccagcaa     1020

cttcgcgacc acggatctcg atgctgaccg cgggaagttg cccggcaaga agctgccgct     1080

ggaggtgctc aaagagatgg aagccaatgc ccggaaagct ggctgcacca ggggctgtct     1140

gatctgcctg tcccacatca agtgcacgcc caagatgaag aagttcatcc caggacgctg     1200

ccacacctac gaaggcgaca aagagtccgc acagggcggc ataggcgagg cgatcgtcga     1260

cattcctgag attcctgggt tcaaggactt ggagcccatg gagcagttca tcgcacaggt     1320

cgatctgtgt gtggactgca caactggctg cctcaaaggg cttgccaacg tgcagtgttc     1380

tgacctgctc aagaagtggc tgccgcaacg ctgtgcgacc tttgccagca agatccaggg     1440

ccaggtggac aagatcaagg gggccggtgg tgactaagcg gccgctcgag catgcatcta     1500

gaaataattc ttactgtcat gccaagtaag atgcttttct gtgctgcaat agcaggcatg     1560

ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg     1620

ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca     1680

gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct     1740

ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt     1800

tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac     1860

gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct     1920

ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt     1980

ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac     2040

aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc     2100

aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt     2160

gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt     2220

cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg     2280

cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct     2340

ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca     2400

aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat     2460

cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga     2520

ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc     2580

ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga     2640

atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg     2700

cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc     2760

cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg     2820

atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga     2880

aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc     2940

tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca     3000

tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg     3060

tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct     3120

atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg     3180

accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc     3240

gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac     3300

gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt     3360

cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga     3420

gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag     3480

catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa     3540

actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta     3600

atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat     3660

acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt     3720

aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta     3780

atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc     3840

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     3900

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     3960

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     4020

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     4080

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     4140

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     4200

tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     4260

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     4320

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     4380

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     4440

cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     4500

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     4560

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac     4620

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc     4680

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     4740

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc     4800

agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac     4860

gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc     4920

accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg     4980

tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag     5040

tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc     5100

acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac     5160

atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag     5220

aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac     5280

tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg     5340

agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc     5400

gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact     5460

ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg     5520

atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa     5580

tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt     5640

tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg     5700

tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga     5760

cgtc                                                                  5764


<210>  8
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer

<400>  8
ggaggtgctc aaagagatgg                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer

<400>  9
ttgaacccag gaatctcagg                                                   20


